Hollow Patents (Class 435/400)
  • Patent number: 10533148
    Abstract: A membrane photobioreactor for treating nitrogen and phosphorus that are out of limits in a biogas slurry and treating method thereof, relating to biogas slurry treatment. The membrane photobioreactor for treating nitrogen and phosphorus that are out of limits in a biogas slurry is provided with a biogas slurry storage tank, peristaltic pumps, a microalgae cultivating tank, an air pump, a membrane photobioreactor and a hollow fiber membrane.
    Type: Grant
    Filed: July 30, 2014
    Date of Patent: January 14, 2020
    Assignee: XIAMEN UNIVERSITY
    Inventors: Qingbiao Li, Xi Chen, Ning He, Yuanpeng Wang, Liang Shen, Haitao Wang
  • Patent number: 8975074
    Abstract: Bone cages are disclosed including devices for biocompatible implantation. The structures of bone are useful for providing living cells and tissues as well as biologically active molecules to subjects.
    Type: Grant
    Filed: October 15, 2007
    Date of Patent: March 10, 2015
    Assignee: The Invention Science Fund I, LLC
    Inventors: Ed Harlow, Edward K. Y. Jung, Robert Langer, Eric C. Leuthardt, Lowell L. Wood, Jr.
  • Publication number: 20150024492
    Abstract: Cell expansion systems and methods of use are provided. The cell expansion systems generally include a hollow fiber cell growth chamber, and first and second circulation loops (intracapillary loops and extracapillary loops) associated with the interior of the hollow fibers and exterior of the hollow fibers, respectively. Detachable flow circuits and methods of expanding cells are also provided.
    Type: Application
    Filed: July 21, 2014
    Publication date: January 22, 2015
    Applicant: TERUMO BCT, INC.
    Inventor: Glen Delbert ANTWILER
  • Patent number: 8796023
    Abstract: A nanostructure composed of a plurality of peptides, each peptide containing at least one aromatic amino acid, whereby one or more of these peptides is end-capping modified, is disclosed. The nanostructure can take a tubular, fibrillar, planar or spherical shape, and can encapsulate, entrap or be coated by other materials. Methods of preparing the nanostructure, and devices and methods utilizing same are also disclosed.
    Type: Grant
    Filed: July 26, 2010
    Date of Patent: August 5, 2014
    Assignee: Ramot at Tel-Aviv University Ltd.
    Inventors: Meital Reches, Ehud Gazit
  • Patent number: 8735143
    Abstract: The present invention provides a hepatic lobule-like bioreactor. The bioreactor includes a nanofiber scaffold enclosed within a housing. An intrahepatic fibrous vascular network, a bile capillary network, upper hepatic bile ducts, lower hepatic bile ducts, a common bile duct connecting the upper and the lower hepatic bile ducts, and collagen fibrous microchannels for hepatocytes surrounded by the bile capillary network are distributed throughout the nanofiber scaffold. Bile capillaries in the bile capillary network are provided with two or more inlet ports for biliary epithelial cells. The collagen fibrous microchannels for hepatocytes are provided with two or more inlet ports for hepatocytes. The intrahepatic vascular network is provided with a liquid inlet port and a liquid outlet port. These ports extend through the housing.
    Type: Grant
    Filed: March 21, 2011
    Date of Patent: May 27, 2014
    Assignee: Zhejiang University
    Inventors: Lanjuan Li, Chengbo Yu, Yiming Zhang, Xiaoping Pan, Weibo Du, Hongcui Cao, Guoliang Lv, Jianrong Huang
  • Patent number: 8696909
    Abstract: A bioartificial kidney equivalent and a process for producing the bioartificial kidney equivalent. The hybrid bioartificial kidney comprises human proximal and distal renal tubule cells grown on particular synthetic membranes.
    Type: Grant
    Filed: September 23, 2009
    Date of Patent: April 15, 2014
    Assignee: Gambro Lundia AB
    Inventors: David Luttropp, Bernd Krause, Markus Neubauer, Andrea Schnell, Helmut Geiger, Juergen Bereiter-Hahn, Patrick Baer, Doris Deppisch
  • Publication number: 20140087466
    Abstract: A fiber includes one or more layers of polymer surrounding a central lumen, and living animal cells disposed within the lumen and/or within at least one of the one or more layers, wherein the fiber has an outer diameter of between 5 and 8000 microns and wherein each individual layer of polymer has a thickness of between 0.1 and 250 microns. Also disclosed are model tissues including such fibers, and method of making such fibers. The fibers can serve as synthetic blood vessels, ducts, or nerves.
    Type: Application
    Filed: March 4, 2013
    Publication date: March 27, 2014
    Inventors: Frances S. Ligler, André A. Adams, Michael Daniele, David R. Mott, Peter B. Howell, JR., Stephanie Fertig, Aron Bobrowski
  • Patent number: 8541196
    Abstract: The present invention provides a device for activating a self-contained biological indicator. The biological indicator includes a casing, an ampule having a growth-promoting medium disposed therein and microorganisms. The ampule and microorganisms are disposed within the casing. The device is comprised of a first lever arm having a cavity formed therein. The cavity is dimensioned to receive a biological indicator. A second lever arm has a protrusion extending from a surface thereof and is moveable relative to the first lever arm to deform a casing of the biological indicator thereby fracturing an ampule in the casing and exposing microorganisms in the casing to a growth-promoting medium in the ampule of the biological indicator.
    Type: Grant
    Filed: April 4, 2012
    Date of Patent: September 24, 2013
    Assignee: American Sterilizer Company
    Inventors: Joseph T. Sestak, Peter J. Buczynski
  • Patent number: 8486699
    Abstract: Two cell lines, PICM-19H and PICM-19B, were derived from the bipotent ARS-PICM-19 pig liver stem cell line. The unipotent porcine stem cell line PICM-19H differentiates exclusively into hepatocytes and can be induced to express CYP450 enzymes. The growth rate and cell density in culture, morphological features, and hepatocyte detoxification functions, i.e., inducible CYP450 activity, ammonia clearance, and urea production of the PICM-19H cells were evaluated for their application in artificial liver devices. PICM-19H cells contain numerous mitochondria, Golgi apparatus, smooth and rough endoplasmic reticulum, vesicular bodies and occasional lipid vacuoles and display inducible CYP450 activity, clear ammonia, and produce urea in a glutamine-free medium. The data indicate that both cell lines, either together or alone, may be useful as the cellular substrate for an artificial liver device. The results demonstrate the potential for the use of PICM-19H cells in drug biotransformation and toxicity testing.
    Type: Grant
    Filed: May 23, 2008
    Date of Patent: July 16, 2013
    Assignee: The United States of America as Represented by the Secretary of Agriculture
    Inventors: Neil C. Talbot, Thomas J. Caperna, Ryan Willard
  • Patent number: 8354258
    Abstract: Diatom devices are disclosed including devices for biocompatible implantation. The diatom structures are useful for providing living cells and tissues as well as biologically active molecules to subjects.
    Type: Grant
    Filed: January 31, 2007
    Date of Patent: January 15, 2013
    Assignee: The Invention Science Fund I, LLC
    Inventors: Edward K. Y. Jung, Robert Langer, Eric C. Leuthardt
  • Publication number: 20120308531
    Abstract: The invention is directed to producing large numbers of cells using hollow fiber bioreactor technology. The cells are non-embryonic stem, non-germ cells that can be characterized by one or more of the following: extended replication in culture and markers of extended replication, such as telomerase, markers of pluripotentiality, and broad differentiation potential, without being transformed.
    Type: Application
    Filed: June 6, 2012
    Publication date: December 6, 2012
    Applicant: ReGenesys BVBA
    Inventors: Jozef Albert Martha Pinxteren, David Craeye
  • Patent number: 8304228
    Abstract: A manipulation tool is disclosed for producing cell material having multiple biological cells, which have a predefined geometrical arrangement. The tool includes a tool body, whose surface at least partially contacts the cell material, and a setting device for adjusting the tool body by a continuous expansion, so that geometrical properties of the surface change and an interior of the tool body is enlarged. The setting device is adapted to expand the tool body at an advance velocity in a range from 0.1 ?m/h to 1 mm/h.
    Type: Grant
    Filed: March 8, 2010
    Date of Patent: November 6, 2012
    Assignee: Fraunhofer-Gesellschaft zur Foerderung der Angewandten Forschung e.V.
    Inventors: Günter R. Fuhr, Heiko Zimmermann
  • Publication number: 20120276573
    Abstract: In accordance with the purpose(s) of the present disclosure, as embodied and broadly described herein, embodiments of the present disclosure, in one aspect, relate to methods of making a structure including nanotubes, a structure including nanotubes, methods of delivering a fluid to a cell, methods of removing a fluid to a cell, methods of accessing intracellular space, and the like.
    Type: Application
    Filed: April 25, 2012
    Publication date: November 1, 2012
    Applicant: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Jules J. VanDersarl, Alexander M. Xu, Nicholas A. Melosh
  • Patent number: 8222001
    Abstract: The invention relates to a process for the culturing of cells, preferably E1-immortalized HER cells, more preferably PER.C6 cells in a reactor in suspension in a cell culture medium, wherein the cells produce a biological substance, preferably an antibody, wherein at least one cell culture medium component is fed to the cell culture and wherein the cell culture comprising the cells, the biological substance and cell culture medium is circulated over a separation system and wherein the separation system separates the biological substance from substances having a lower molecular weight than the biological substance and wherein the biological substance is retained in or fed back into the reactor. Preferably part of the substances of lower molecular weight is continuously removed from the cell culture.
    Type: Grant
    Filed: February 13, 2012
    Date of Patent: July 17, 2012
    Assignee: DSM IP Assets B.V.
    Inventors: Gerben Meile Zijlstra, Robert Patrick Hoff, Jacob Schilder
  • Publication number: 20120145626
    Abstract: A device for culturing, storing and expanding adherent renal cells. The device permits the adhesion and proliferation of the cells and the surface characteristics of the membrane material used in said device further permit the cellular matrix components (EMC). A method for expanding adherent renal cells and methods of using particular membranes for culturing, storing and expanding adherent renal cells. The membranes comprise a copolymer of acrylonitrile and sodium methallyl sulfonate as well as polyethylenimine.
    Type: Application
    Filed: September 23, 2009
    Publication date: June 14, 2012
    Applicant: GAMBRO LUNDIA AB
    Inventors: David Luttropp, Markus Neubauer, Helmut Geiger, Juergen Bereiter-Hahn, Patrick Baer, Reinhold Deppisch, Doris Deppisch
  • Patent number: 8119368
    Abstract: The invention relates to a process for the culturing of cells, preferably E1-immortalized HER cells, more preferably PER.C6 cells in a reactor in suspension in a cell culture medium, wherein the cells produce a biological substance, preferably an antibody, wherein at least one cell culture medium component is fed to the cell culture and wherein the cell culture comprising the cells, the biological substance and cell culture medium is circulated over a separation system and wherein the separation system separates the biological substance from substances having a lower molecular weight than the biological substance and wherein the biological substance is retained in or fed back into the reactor. Preferably part of the substances of lower molecular weight is continuously removed from the cell culture.
    Type: Grant
    Filed: July 4, 2007
    Date of Patent: February 21, 2012
    Assignee: DSM IP Assets B.V.
    Inventors: Gerben Meile Zijlstra, Robert Patrick Hof, Jacob Schilder
  • Publication number: 20120040463
    Abstract: The present invention relates to a method for preparing fibers, notably of polysaccharide or collagen, by wet spinning under coagulation, said method notably comprising: a step for extruding a coagulable macromolecular assembly solution through a normal die; at least one partial coagulation cycle comprising a coagulation step on the one hand and a step for interrupting the coagulation on the other hand; and a step for receiving, notably by winding up, the obtained hollow fiber. The invention also relates to multi-membrane hollow fibers consisting of a same macromolecular assembly which may be spun by coagulation, notably a natural or modified natural polysaccharide in the physical hydrogel or partly dehydrated state. Said fibers including at least over their length two superposed coaxial membranes separated from each other by an inter-membrane space. The invention also relates to the spinning device for applying said method.
    Type: Application
    Filed: September 5, 2008
    Publication date: February 16, 2012
    Applicants: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, UNIVERSITE CLAUDE BERNARD LYON I
    Inventors: Alain Domard, Laurent David, Rocío Nohemí Rivas Araiza
  • Patent number: 8062873
    Abstract: A membrane supported bioreactor arrangement and method for anerobic conversion of gas into liquid products including membrane modules having hollow fibers, each of the hollow fibers formed from an asymmetric membrane wall having a porous outer layer defining biopores for retaining a porous biolayer about the outer surface of the membrane wall and a less permeable hydration layer around the hollow fiber lumen; a membrane vessel for retaining the membrane modules in a process gas for formation of the biolayer on the outer surface of the hollow fiber wall by interaction of microorganisms with a process gas and for the production of a liquid product, wherein the membrane vessel retains the membrane modules in a common horizontal plane; provides a seal between contents of the membrane tank and ambient atmosphere; and includes a liquid supply conduit for communicating the process liquid with the hollow fiber lumens of the hollow fibers.
    Type: Grant
    Filed: October 24, 2008
    Date of Patent: November 22, 2011
    Assignee: Coskata Energy
    Inventors: Shih-Perng Tsai, Rathin Datta, Rahul Basu, Seong-Hoon Yoon
  • Patent number: 8013113
    Abstract: The present invention provides clostridial toxin substrates useful in assaying for the protease activity of any clostridial toxin, including botulinum toxins of all serotypes as well as tetanus toxins. A clostridial toxin substrate of the invention contains a donor fluorophore; an acceptor having an absorbance spectrum overlapping the emission spectrum of the donor fluorophore; and a clostridial toxin recognition sequence that includes a cleavage site, where the cleavage site intervenes between the donor fluorophore and the acceptor and where, under the appropriate conditions, resonance energy transfer is exhibited between the donor fluorophore and the acceptor.
    Type: Grant
    Filed: November 17, 2009
    Date of Patent: September 6, 2011
    Assignee: Allergan, Inc.
    Inventors: Lance E. Steward, Ester Fernandez-Salas, Kei Roger Aoki
  • Publication number: 20110091926
    Abstract: A perfusable bioreactor is used for the production of human tissues, animal tissues or tissue equivalents. Their production is based on a structure cultivated in the interior, the interior being enclosed by an envelope and containing at least one inlet and one outlet for a liquid nutrient medium. Accordingly, the bioreactor can be connected to a unit for producing a perfusion pressure of the nutrient medium. This tissue replacement is particularly suitable for use in clinical/therapeutical applications.
    Type: Application
    Filed: March 23, 2009
    Publication date: April 21, 2011
    Applicant: NOVATISSUE GMBH
    Inventor: Bernhard Frerich
  • Patent number: 7874998
    Abstract: The present invention relates to filtration devices and related methods of use. In particular, the present invention relates to implantable filtration devices used, for example, for filtering impurities from a body fluid of a subject.
    Type: Grant
    Filed: November 6, 2006
    Date of Patent: January 25, 2011
    Assignee: The Regents of the University of Michigan
    Inventors: H. David Humes, David L. Brown
  • Patent number: 7709608
    Abstract: The present invention provides clostridial toxin substrates useful in assaying for the protease activity of any clostridial toxin, including botulinum toxins of all serotypes as well as tetanus toxins. A clostridial toxin substrate of the invention contains a donor fluorophore; an acceptor having an absorbance spectrum overlapping the emission spectrum of the donor fluorophore; and a clostridial toxin recognition sequence that includes a cleavage site, where the cleavage site intervenes between the donor fluorophore and the acceptor and where, under the appropriate conditions, resonance energy transfer is exhibited between the donor fluorophore and the acceptor.
    Type: Grant
    Filed: December 11, 2006
    Date of Patent: May 4, 2010
    Assignee: Allergan, Inc.
    Inventors: Lance E. Steward, Ester Fernandez-Salas, Kei Roger Aoki
  • Patent number: 7704741
    Abstract: A method for producing cell material (20) having multiple biological cells (21), which have a predefined geometrical arrangement, includes the steps of providing a manipulation tool (10) having a tool body (11), whose surface (12, 14) at least partially contacts the cell material (20), and adjusting the manipulation tool (10) using a change of geometrical properties of the surface (12, 14) in such a way that the geometrical arrangement of the cells (21) is changed. A manipulation tool for performing a method of this type is also described.
    Type: Grant
    Filed: December 2, 2003
    Date of Patent: April 27, 2010
    Assignee: Fraunhofer-Gesellschaft zur Förderung der Angewandten Forschung e.V.
    Inventors: Gunter R. Fuhr, Heiko Zimmermann
  • Patent number: 7695958
    Abstract: To provide a cell-filled device that is suitable for use in, for example, an implantable or circulation type hybrid artificial organ. In a cell-filled device including hollow fiber membranes whose hollow portions are filled with cells, the hollow fiber membranes have modified cross sections, and a cell aggregate provided in each of the hollow portions has cells formed into two or more layers in arbitrary directions, provided that the distance from an arbitrary point of the cell aggregate to the nearest inner wall of hollow fiber membrane is less than 75 ?m. This cell-filled device enables effective use of cells without the necrosis thereof. Further, according to the present invention, there is provided a method of manufacturing the cell-filled device.
    Type: Grant
    Filed: August 28, 2003
    Date of Patent: April 13, 2010
    Assignee: Asahi Kasei Kuraray Medical Co., Ltd.
    Inventors: Kazumori Funatsu, Kohji Nakazawa, Hiroshi Mizumoto, Junji Fukuda, Mikitomo Yasutake
  • Publication number: 20090136553
    Abstract: Provided are tubular structures of a biocompatible, triggerably-dissolvable material such as cellulose or a copolymer having an LCST below physiological temperatures. The structures may be embedded within a cell growth scaffold. The tubular structures are useful in growing 3-dimensional tissue structures because nutrients, cytokines or other cell growth and/or differentiation compounds, as well as drugs, such as antibiotics and steroids, can be administered over time, and the tubular structures can be dissolved non-invasively.
    Type: Application
    Filed: September 25, 2008
    Publication date: May 28, 2009
    Inventors: Jorg C. Gerlach, Jianjun Guan, Steven R. Little, Alan J. Russell, Jillian Tengood, William R. Wagner
  • Patent number: 7442546
    Abstract: A method of immunomodulation by contacting the bodily fluid of a patient with renal tubule cells outside of the kidney.
    Type: Grant
    Filed: February 25, 2003
    Date of Patent: October 28, 2008
    Assignee: The Regents of the University of Michigan
    Inventor: H. David Humes
  • Publication number: 20080248572
    Abstract: The present invention relates to treatment of polymeric bioreactor surfaces, to promote the proliferation of adherent cells.
    Type: Application
    Filed: March 5, 2008
    Publication date: October 9, 2008
    Applicant: GAMBRO BCT, INC.
    Inventor: Glen Delbert ANTWILER
  • Patent number: 7354773
    Abstract: A method and apparatus for preparing biological cell samples for intracellular analysis. The invention is based upon the recognition that many of the steps of the conventional methods for such sample preparation can be eliminated, leading to a process that readily lends itself to automation and the advantages associated therewith. The method of the invention comprises the steps of (a) cell-fixation, (b) permeabilization and (c) staining (or labeling) of intracellular molecules of interest by probes that are readily detectable by flow cytometric techniques, all without any intervening cell-washing (and re-suspension) steps. Rather, the single cell-washing step is effected after these three steps have been carried out. Preferably, the washing step is carried out by passing the fixed, permeabilized and stained cell sample through a semi-permeable membrane that serves to filter out (by transmission) interferants to waste while retaining the cells of interest.
    Type: Grant
    Filed: May 14, 2003
    Date of Patent: April 8, 2008
    Assignee: Beckman Coulter, Inc.
    Inventors: Oilda Rubio, Carlos Aparicio, John A. Maples, Julie Wilkinson, Cecilia Smith, Frank J. Lucas
  • Patent number: 7008634
    Abstract: Described are compositions with tethered growth effector molecules, and methods of using these compositions for growing cells and tissues. Growth effector molecules, including growth factors and extracellular matrix molecules, are flexibly tethered to a solid substrate. The compositions can be used either in vitro or in vivo to grow cells and tissues. By tethering the growth factors, they will not diffuse away from the desired location. By making the attachment flexible, the growth effector molecules can more naturally bind to cell surface receptors. A significant feature of these compositions and methods is that they enhance the biological response to the growth factors. The method also offers other advantages over the traditional methods, in which growth factors are delivered in soluble form: (1) the growth factor is localized to a desired target cell population; (2) significantly less growth factor is needed to exert a biologic response.
    Type: Grant
    Filed: March 3, 1995
    Date of Patent: March 7, 2006
    Assignee: Massachusetts Institute of Technology
    Inventors: Linda G. Cima, Edward W. Merrill, Philip R. Kuhl
  • Patent number: 6960427
    Abstract: The invention concerns a process for producing a three-dimensional bioartificial tissue having viable cells in or on a matrix, and by which cells and matrix can be cultivated into a tissue or a precursor of a tissue, a vascularized tissue of biological materials, obtained by this process, and an experimental reactor for scientific purposes and for producing clinically usable tissues and organs.
    Type: Grant
    Filed: February 28, 2002
    Date of Patent: November 1, 2005
    Assignee: Artiss GmbH
    Inventors: Axel Haverich, Theo Kofidis
  • Patent number: 6835566
    Abstract: A method for obtaining lineage committed human cells imbued with enhanced proliferative potential, biological function, or both, comprising culturing lineage committed human cells under physiologically acceptable liquid culture conditions, where the liquid culture medium is replaced at a rate and for a time sufficient to obtain the human lineage committed cells imbued with enhanced proliferative potential, biological function, or both; and isolating the cultured cells.
    Type: Grant
    Filed: June 29, 2001
    Date of Patent: December 28, 2004
    Assignee: Aastrom Biosciences, Inc.
    Inventors: Alan K. Smith, Douglas M. Smith, Ramkumar K. Mandalam
  • Publication number: 20040203147
    Abstract: A method and structure for growing living organic tissue in which a scaffold is provided on which cells of the tissue may be seeded and on which the tissue may be grown and a plurality of membrane capillaries are provided for conveying a nutrient fluid, the plurality of membrane capillaries being interspersed through the scaffold and having membranes which are permeable to nutrients and oxygen such that the tissue may be grown throughout the scaffold and whereby upon in vivo implantation a supporting nutrient tissue supply rapidly establishes.
    Type: Application
    Filed: June 14, 2004
    Publication date: October 14, 2004
    Inventors: James Tomlinson Triffitt, Zhidao Xia, Hua Ye, Zhangfeng Cui
  • Patent number: 6787342
    Abstract: This invention provides for a pharmaceutical or veterinary paste formulation comprising: an effective amount of a therapeutic agent; fumed silica; a viscosity modifier; a hydrophilic carrier; optionally, an absorbent; and optionally, a colorant, stabilizer, surfactant, or preservative. This invention also provides for methods of using these formulations for treating various disease states as well.
    Type: Grant
    Filed: February 16, 2000
    Date of Patent: September 7, 2004
    Assignee: Merial Limited
    Inventor: Jun Chen
  • Publication number: 20040048366
    Abstract: The present invention relates to a reactor module constructed of hollow fibers and cells as well as to reactors comprising this reactor module.
    Type: Application
    Filed: June 20, 2003
    Publication date: March 11, 2004
    Inventors: Norbert Stroh, Thomas Graeve
  • Patent number: 6680166
    Abstract: A cell culture unit is disclosed herewith comprising a shell having two ends with an elongated chamber there between. A liquid and a gas perfusion port are located on each end. Liquid perfusion fibers are connected to the liquid perfusion ports and gas perfusion fibers are connected to the gas perfusion ports. These fibers thereby define an extracapillary and intracapillary space. The cell culture unit further comprises means for communicating with the intracapillary space of the gas perfusion fibers means for communicating with the intracapillary space of the liquid perfusion fibers, and means for communicating with the extracapillary space.
    Type: Grant
    Filed: March 29, 1999
    Date of Patent: January 20, 2004
    Inventors: Claudy Jean Paul Mullon, Kerry Ann Gagnon, Christine Marie Tosone, Hugo Osvaldo Jauregui
  • Patent number: 6667172
    Abstract: A cell and tissue culture modeling device comprising a housing having an interior chamber, an inlet port in fluid communication with the internal chamber, an outlet port in fluid communication with the internal chamber, a plurality of hollow fibers disposed within the interior chamber and traversing the length of the housing between the inlet port and the outlet port. Each of the plurality of hollow fibers has an interior defining an intracapillary space and the interior chamber defines an extracapillary space unoccupied by the plurality of hollow fibers. To access the extracapillary space of the device, a portion of the housing is removable. The device can be used to conduct permeability, drug efficacy, and gene expression studies.
    Type: Grant
    Filed: September 19, 2001
    Date of Patent: December 23, 2003
    Assignee: The Cleveland Clinic Foundation
    Inventors: Damir Janigro, Mark S. McAllister
  • Patent number: 6653131
    Abstract: A method for treating systemic inflammatory response syndrome (SIRS) by contacting the bodily fluid of a patient with renal tubule cells outside of the kidney.
    Type: Grant
    Filed: August 30, 2001
    Date of Patent: November 25, 2003
    Assignee: The Regents of the University of Michigan
    Inventor: H. David Humes
  • Patent number: 6616912
    Abstract: A bi-component microporous hollow fiber membrane structure is provided for in vivo propagation of cells and use in testing of the effect of medical treatments on cells within the structure. The structure has an inner structure fabricated from a first bio-compatible polymer and an outer structure fabricated from a different polymer that has a lower tendency for cell adhesion than the inner structure polymer. In this way, the inner structure can be selected to optimize cell propagation and the outer structure can be fabricated from a polymer which optimizes the removal of the bi-component structure from its implanted location. The inner and outer structures may have a pore size between 10 and 1000 Angstroms and 100 and 2000 Angstroms, respectively, and be formed from polysulfone or polyether sulfone and polyvinyledene difuoride, respectively. The membrane structure can form macrocapsules containing media and living cells for implanting.
    Type: Grant
    Filed: January 5, 2001
    Date of Patent: September 9, 2003
    Assignee: Spectrum Laboratories, Inc.
    Inventors: Roy T. Eddleman, Jesus Martinez
  • Publication number: 20030054545
    Abstract: A cell and tissue culture modeling device comprising a housing having an interior chamber, an inlet port in fluid communication with the internal chamber, an outlet port in fluid communication with the internal chamber, a plurality of hollow fibers disposed within the interior chamber and traversing the length of the housing between the inlet port and the outlet port. Each of the plurality of hollow fibers has an interior defining an intracapillary space and the interior chamber defines an extracapillary space unoccupied by the plurality of hollow fibers. To access the extracapillary space of the device, a portion of the housing is removable. The device can be used to conduct permeability, drug efficacy, and gene expression studies.
    Type: Application
    Filed: September 19, 2001
    Publication date: March 20, 2003
    Inventors: Damir Janigro, Mark S. McAllister
  • Publication number: 20030017591
    Abstract: The present invention provides novel isothermal, single primer linear nucleic acid amplification methods. Methods for amplifying complementary DNA using a composite primer, primer extension, strand displacement, and optionally a termination sequence, are provided. Methods for amplifying sense RNA using a composite primer, primer extension, strand displacement, optionally template switching, a propromoter oligonucleotide and transcription are also provided. The invention further provides compositions and kits for practicing said methods, as well as methods which use the amplification products.
    Type: Application
    Filed: November 21, 2001
    Publication date: January 23, 2003
    Inventor: Nurith Kurn
  • Publication number: 20020168758
    Abstract: A bioreactor having an outer shell compartment containing many coaxial pairs of hollow microporous fibers. An annular compartment exists between an outer surface of the inner hollow microporous fiver and the inner surface of the outer hollow microporous fiber. The annular compartment preferably contains blood or plasma. The blood or plasma is, thus, exposed to two microporous hollow fibers which have fluids which can purify or otherwise affect the blood through the walls of two different microporous hollow fibers. An artificial liver or kidney can result when liver or kidney cells are placed in the shell compartment.
    Type: Application
    Filed: May 11, 2001
    Publication date: November 14, 2002
    Inventors: F. Jesus Martinez, Virginia Thanh Ta
  • Patent number: 6464998
    Abstract: The present invention provides cell compositions for in vivo implantation, and designed for the sustained and controlled delivery of therapeutic substances.
    Type: Grant
    Filed: July 11, 1996
    Date of Patent: October 15, 2002
    Assignee: Aventis Pharma S.A.
    Inventors: Yves Beuzard, Olivier Danos, Vincent Descamps, Jean-Michel Heard, Philippe Moullier, Nadia Naffakh, Michel Perricaudet, William Vainchenker
  • Publication number: 20020137205
    Abstract: The invention relates to the use of microscopic nematodes such as C. elegans in functional high throughput in vivo assays suitable for the detection of inhibitors or activators of intestinal lipid uptake.
    Type: Application
    Filed: May 21, 2001
    Publication date: September 26, 2002
    Inventors: Philippe Verwaerde, Cindy Anthonissen, Benoit Deprez, Beatrice Bonnet, Thierry Bogaert
  • Publication number: 20020127720
    Abstract: Biologically active composites containing calcium phosphate, more specifically beta-tri-calcium phosphate, and methods of preparing the same are disclosed herein. Also disclosed are methods of restoring an osseous void and complimentary kits for the disclosed composites.
    Type: Application
    Filed: October 23, 2001
    Publication date: September 12, 2002
    Inventors: Erik M. Erbe, Jeffrey G. Marx
  • Publication number: 20020086424
    Abstract: The present invention relates to biodetectors for detecting and quantifying molecules in liquid, gas, or matrices. More specifically, the present invention relates to biodetectors comprising a molecular switching mechanism to express a reporter gene upon interaction with target substances. The invention further relates to methods using such biodetectors for detecting and quantifying selected substances with high specificity and high sensitivity.
    Type: Application
    Filed: October 30, 1998
    Publication date: July 4, 2002
    Inventors: PAMELA R. CONTAG, CHRISTOPHER H. CONTAG, DAVID A. BENARON
  • Publication number: 20020076815
    Abstract: A bio-reactor for enhancing the biomass yield using a closed sterile vessel having a medium, said reactor comprising a central shaft, a support matrix oriented horizontally and dividing the vessel into an upper and a lower chamber, a means for stirring selected from turbine-2,4,6-impeller; marine blade impeller; helical blade impeller; mounted onto the central shaft and located at a predetermined distance from the support matrix so as to generate both radially and axially directed volumetric flow of the media; and at least two semi-circular sparges located both in the upper and lower compartments of the vessel at a predetermined distance from the support matrix.
    Type: Application
    Filed: December 15, 2000
    Publication date: June 20, 2002
    Applicant: COUNCIL OF SCIENTIFIC & INDUSTRIAL RESEARCH
    Inventors: Suchitra Banerjee, Arun Kumar Kukreja, Praveen Chandra Verma, Atul Prakash Kahol, Sushil Kumar
  • Patent number: 6379963
    Abstract: The invention concerns a process for producing a three-dimensional bioartificial tissue having viable cells in or on a matrix, and by which cells and matrix can be cultivated into a tissue or a precursor of a tissue, a vascularized tissue of biological materials, obtained by this process, and an experimental reactor for scientific purposes and for producing clinically usable tissues and organs.
    Type: Grant
    Filed: January 16, 2001
    Date of Patent: April 30, 2002
    Inventors: Axel Haverich, Theo Kofidis
  • Patent number: 6352859
    Abstract: The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.
    Type: Grant
    Filed: July 26, 2000
    Date of Patent: March 5, 2002
    Assignee: Maxygen, Inc.
    Inventors: Stephen delCardayre, Matthew Tobin, William P. C. Stemmer, Jon E. Ness, Jeremy Minshull, Phillip Patten, Venkiteswatan Subramanian, Linda Castle, Claus M. Krebber, Steve Bass
  • Patent number: 6306644
    Abstract: A process and device are disclosed for simultaneously cultivating different mammal cells, for separately obtaining different mammal cell products and for simulating organic interactions on the humoral plane. Essentially, the invention consists of arranging several culture vessels in a common supply circuit and or cultivating different mammal cells in separate vessels.
    Type: Grant
    Filed: March 12, 2001
    Date of Patent: October 23, 2001
    Inventors: Uwe Marx, Gert Hausdorf
  • Patent number: 6287863
    Abstract: Improved recombinant retrotransposon vectors for gene transfer are disclosed. The synthetic vectors are truncated so as to reduce or altogether eliminate homologous recombination with retroviral helper sequences found in helper cells used to propagate the vectors, making them safer for use in humans and providing more space for therapeutic genes. The vectors transmit foreign DNA efficiently, are stable, enable abundant RNA expression from the retrotransposon transcriptional promoter, and through their diversity permit many useful applications in therapeutics and transgenics. Methods are described for rescuing tissue-specifics promoters obtaining expression in primary cells, mapping the genome and other techniques of therapeutic and transgenic utility.
    Type: Grant
    Filed: November 9, 1995
    Date of Patent: September 11, 2001
    Assignee: Nature Technology Corporation
    Inventor: Clague P. Hodgson