Drug Or Compound Screening Involving Gene Expression Patents (Class 435/6.13)
  • Patent number: 11001868
    Abstract: Methods for in vitro transcription and translation using a double-stranded concatemeric DNA in a eukaryotic cell-free expression system are provided. The method includes the steps of (a) contacting a double-stranded concatemeric DNA with a eukaryotic cell-free expression system, and (b) expressing a protein in vitro from the double-stranded concatemeric DNA in the eukaryotic cell-free expression system. The double-stranded concatemeric DNA includes a plurality of tandem repeat sequences. The plurality of tandem repeat sequences includes an expression sequence including a promoter, a cap-independent translation element (CITE), and an open reading frame. A final concentration of the double-stranded concatemeric DNA in the eukaryotic cell-free expression system is in a range from about 0.1 ng/?L to about 35 ng/?L. A RCA product DNA may be used as the double stranded concatemer DNA for the methods.
    Type: Grant
    Filed: August 11, 2017
    Date of Patent: May 11, 2021
    Assignee: Global Life Sciences Solutions Operations UK Ltd
    Inventors: Erik Leeming Kvam, John Richard Nelson, Wei Gao
  • Patent number: 11001881
    Abstract: This invention provides methods and systems for measuring the concentration of multiple nucleic acid sequences in a sample. The nucleic acid sequences in the sample are simultaneously amplified, for example, using polymerase chain reaction (PCR) in the presence of an array of nucleic acid probes. The amount of amplicon corresponding to the multiple nucleic acid sequences can be measured in real-time during or after each cycle using a real-time microarray. The measured amount of amplicon produced can be used to determine the original amount of the nucleic acid sequences in the sample. Also provided herein are biosensor arrays, systems and methods for affinity based assays that are able to simultaneously obtain high quality measurements of the binding characteristics of multiple analytes, and that are able to determine the amounts of those analytes in solution. The invention also provides a fully integrated bioarray for detecting real-time characteristics of affinity based assays.
    Type: Grant
    Filed: August 29, 2017
    Date of Patent: May 11, 2021
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Arjang Hassibi, Babak Hassibi, Haris Vikalo
  • Patent number: 10988740
    Abstract: Methods and compositions are provided for engineering microorganisms, which permit enhanced H2 production. The methods and compositions provided include novel chimeric gene constructs encoding H2-forming H2ase and maturation proteins, allowing for generation of H2 continuously in large quantities. In one illustrated embodiment, novel engineered algae are provided with increased levels of H2 production.
    Type: Grant
    Filed: November 14, 2014
    Date of Patent: April 27, 2021
    Inventor: Svetlana Oard
  • Patent number: 10982257
    Abstract: Method for studying constituents of individual molecular complexes by labelling the molecules belonging to the same complex with at least one set of molecular constructs, wherein each set member includes a Unique Identifying Sequence (UIS), which is a nucleic acid sequence unique for each set member, and at least one Common Tag Sequence (CTS), which is a nucleic acid sequence common to all set members, by: attaching the molecular construct to the complex by ligating or hybridizing the molecular construct to a nucleic acid molecule of the complex, or ligating or hybridizing the tag to a nucleic acid linked to an affinity binder that binds specifically to a constituent of the complex; labelling the molecules belonging to the same complex using the molecular construct tags as primers or templates in a nucleic acid polymerization reaction; and analyzing the composition of the complex by analyzing combinations of UISs and CTSs.
    Type: Grant
    Filed: March 23, 2020
    Date of Patent: April 20, 2021
    Assignee: VESICODE AB
    Inventor: Di Wu
  • Patent number: 10976313
    Abstract: Multiplex immunoassays utilize the differential affinities among the conjugation pairs between the capture ligands and target analytes are proposed. Window magnetic-assisted rapid aptamer selection (window-MARAS) methods for selecting aptamers with desirable affinity toward the target analytes and methods for generating reagents for multiplex immunoassays or multiplex detection in one assay by utilizing the selected aptamers as capture ligands in reagents are described and used to demonstrate the feasibility of multiplex immunoassays based on the differential affinity of conjugation pairs between the capture ligands and target analytes.
    Type: Grant
    Filed: November 22, 2018
    Date of Patent: April 13, 2021
    Inventors: Chin-Yih Hong, Herng-Er Horng
  • Patent number: 10961499
    Abstract: The present invention relates to a new method for the production of a molecule of interest by conversion of a source of carbon in a fermentative process comprising culturing a microorganism genetically modified for the production of molecule of interest, wherein said microorganism comprises functional genes coding PTS carbohydrate utilization system and wherein the expression of proteins regulated the expression of phosphoenolpyruvate synthase (PPS) is down-regulated. The present invention also relates to the genetically modified microorganism used in the method of the invention.
    Type: Grant
    Filed: July 6, 2017
    Date of Patent: March 30, 2021
    Assignee: METABOLIC EXPLORER
    Inventors: Gwénaëlle Corre, Céline Raynaud
  • Patent number: 10945427
    Abstract: The problem of the present invention is to provide a method for preserving mammalian cells over a long period of time using a solution for cell transplantation, capable of effectively suppressing cell death when the mammalian cells have been preserved, and the solution for cell transplantation. The present invention is characterized in that mammalian cells are preserved in a physiological aqueous solution for cell transplantation, comprising 2.0 to 6.0% (w/v) of trehalose, a derivative thereof, or a salt of trehalose or the derivative (a trehalose) and 4.0 to 7.0% (w/v) of dextran, a derivative thereof, or a salt of dextran or the derivative (a dextran). The effects of a trehalose and a dextran contained in the physiological aqueous solution for cell transplantation can suppress a decrease in the cell survival rate when mammalian cells are preserved for a long period of time (at least 14 days).
    Type: Grant
    Filed: June 18, 2014
    Date of Patent: March 16, 2021
    Assignee: OTSUKA PHARMACEUTICAL FACTORY, INC.
    Inventors: Masuhiro Nishimura, Tamaki Wada, Chikage Shirakawa, Masako Doi
  • Patent number: 10881332
    Abstract: The invention relates to carbon nanotube-containing composites as biosensors to detect the presence of target clinical markers, methods of their preparation and uses in the medical field. The invention is particularly suitable for the detection in patient biological specimens of bone markers and tissue markers. The biosensors of the invention include carbon nanotubes deposited on a substrate, gold nanoparticles deposited on the carbon nanotubes and, binder material and biomolecule deposited on the gold-coated carbon nanotubes. The biomolecule is selected to interact with the target clinical markers. The biosensor can be used as an in-situ or an ex-situ device to detect and measure the presence of the target clinical markers.
    Type: Grant
    Filed: August 17, 2017
    Date of Patent: January 5, 2021
    Assignee: UNIVERSITY OF PITTSBURGH—OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION
    Inventors: Prashant Nagesh Kumta, Madhumati Ramanathan, Mitali Shirish Patil
  • Patent number: 10858629
    Abstract: Disclosed is a new process for the production of recombinant proteins, by transient transfection of suspension-grown human embryonic kidney cells (293 cell line and its genetic variants) with an expression vector, using polyethylenimine (PEI) as a transfection reagent. In a preferred embodiment, the process uses 293E cells expressing the Epstein-Barr virus (EBV) EBNA 1 protein, in combination with an oriP-based episomal expression vector having an improved cytomegalovirus expression cassette comprising the CMV5 promoter. The process combines in a single step the cell growth, transfection and protein expression, is carried out without changing the culture medium, and allows to achieve high expression levels in a short period of time. The process may be carried out in a serum-free, low-protein culture medium, is easily scalable, compatible with continuous production processes, and fully adapted to high-throughput production of milligram quantities of recombinant proteins.
    Type: Grant
    Filed: August 27, 2019
    Date of Patent: December 8, 2020
    Assignee: National Research Council of Canada
    Inventors: Yves Durocher, Amine Kamen, Sylvie Perret, Phuong Pham
  • Patent number: 10858693
    Abstract: Biosensors and methods for localized surface plasmon resonance biosensing are disclosed. The biosensor can include a substrate having a substrate surface to which a plurality of localized surface plasmon resonance (LSPR) antennae are affixed. The LSPR antennae can be affixed via an affixation surface of the LSPR antenna. The LSPR antennae can have a functional surface opposite the affixation surface. Each functional surface can be functionalized by a plurality of single-stranded DNA.
    Type: Grant
    Filed: October 6, 2015
    Date of Patent: December 8, 2020
    Assignee: INDIANA UNIVERSITY RESEARCH & TECHNOLOGY CORPORATION
    Inventors: Rajesh Sardar, Murray Korc, Gayatri K. Joshi
  • Patent number: 10837001
    Abstract: Disclosed herein are methods of inducing and/or promoting cardiomyocyte maturation comprising: providing an immature cardiomyocyte; providing a three dimensional (3D) cardiac extracellular matrix (ECM) scaffold; and inducing and/or promoting cardiomyocyte cell maturation by seeding the immature cardiomyocyte in the 3D cardiac ECM scaffold and harvesting once the cardiomyocyte has reached maturity. Also disclosed herein are methods of treating a disease in a mammal comprising transplanting a mature cardiomyocyte into an ischemic heart, wherein the mature cardiomyocyte is generated comprising the steps of: providing an immature cardiomyocyte; providing a 3D cardiac ECM scaffold; and generating mature cardiomyocyte by seeding the immature cardiomyocyte in a 3D cardiac ECM scaffold or co-culturing the immature cardiomyocyte in the presence of endothelial cells or stromal cells; and harvesting once the cardiomyocyte has reached maturity.
    Type: Grant
    Filed: March 2, 2017
    Date of Patent: November 17, 2020
    Assignee: The Regents of The University of California
    Inventors: Ashley H. Fong, Christopher C. W. Hughes
  • Patent number: 10822662
    Abstract: A method for diagnosing a lymphoid proliferative disorder (such as lymphoma, T-cell lymphoma or leukemia) in a subject or determining the risk of a subject for a lymphoid proliferative disorder recurrence after transient remission, which includes high throughput T-Cell Receptor ? (TCR?) sequencing tests to identify dominant oncogenic clones in the subject suffering from the lymphoid proliferative disorder. Oligonucleotides primer compositions and kits associated with the above-described methods are also provided herein. Said subject includes living organisms, such as mammals (e.g., dogs, cats, pigs, cows, horses, goats, rabbits, humans), non-mammalian vertebrates, such as birds (e.g., chicken, ducks), fish (e.g., sharks), or frogs, and transgenic species thereof.
    Type: Grant
    Filed: March 5, 2018
    Date of Patent: November 3, 2020
    Assignee: Karkinos Precision Oncology LLC
    Inventor: Adam Scott Buntzman
  • Patent number: 10808080
    Abstract: A monomer is represented by Chemical Formula 1: wherein, in Chemical Formula 1, R1, R2, o, p, L1, A1, Ra, m, and n are the same as defined in the detailed description.
    Type: Grant
    Filed: July 30, 2018
    Date of Patent: October 20, 2020
    Assignee: SAMSUNG ELECTRONICS CO, LTD.
    Inventors: Dmitry Androsov, Changki Kim, Kalinina Fedosya, Hyunseok Choi
  • Patent number: 10774361
    Abstract: The invention relates to a method for identifying unknown microbes in a sample, wherein a mass spectrometric determination down to the taxonomic level of the genus or species is supplemented by a detailed determination of a lower taxonomic level or variety by means of infrared spectrometry, using restricted reference libraries of infrared spectra. These libraries can be genus-specific, containing only infrared spectra of microbes of one genus, or species-specific, containing only infrared spectra of microbes of one species. In so doing, a robust mass spectrometric identification of the species of unknown microbes is advantageously supplemented with a detailed analysis of the subspecies and varieties by means of infrared spectrometry, primarily in order to identify medically important varieties such as pathovars like EHEC and EPEC, and antibiotic-resistant microbes like MRSA.
    Type: Grant
    Filed: August 31, 2018
    Date of Patent: September 15, 2020
    Inventor: Markus Kostrzewa
  • Patent number: 10767207
    Abstract: Fungal glucoamylases from Aspergillus fumigatus—expressed in Trichoderma reesei host cells (AfGATR) are provided. Trichoderma reesei host cells express AfGATRs at higher, or at least comparable, levels to natively expressed AfGA Aspergillus fumigatus. AfGATRs, including AfGA1TR and AfGA2TR, exhibit high activity at elevated temperatures and at low pH, so AfGATRs can be used efficiently in a process of saccharification in the presence of alpha-amylase, such as Aspergillus kawachii alpha-amylase (AkAA). AfGATRs advantageously catalyze starch saccharification to an oligosaccharide composition significantly enriched in DP1 (i.e., glucose) compared to the products of saccharification catalyzed by Aspergillus niger glucoamylase (AnGA) or native AfGA expressed in Aspergillus fumigatus. AfGATRs such as AfGA1TR, AfGA2TR or a variant thereof can be used at a lower dosage than AnGA and natively expressed AfGAs to produce comparable levels of glucose.
    Type: Grant
    Filed: February 21, 2019
    Date of Patent: September 8, 2020
    Assignee: Danisco US INC
    Inventors: Jing Ge, Ling Hua, Sung Ho Lee, Jalsen Li, Jayarama K Shetty, Zhongmei Tang, Bo Zhang, Kun Zhong
  • Patent number: 10745693
    Abstract: Described herein is a method for identifying synthetic inducible promoters that have specified induction and/or repression for DNA binding proteins such as an allosteric transcription factor and an inducer molecule. The method includes an in vitro selection from an unselected polynucleotide library comprising a plurality of random degeneracies, and an in vivo selection to produce an induced promoter library. Produced is an induction table, which allows the selection of a promoter with specific induction and/or repression properties. Also included are biosensors containing the synthetic inducible promoters.
    Type: Grant
    Filed: April 4, 2018
    Date of Patent: August 18, 2020
    Assignee: WISCONSIN ALUMNI RESEARCH FOUNDATION
    Inventors: Srivatsan Raman, Aseem Zoe Ansari, Xiangyang Liu, Jose Arcadio Rodriguez-Martinez
  • Patent number: 10699800
    Abstract: Provided herein are methods, processes and apparatuses for non-invasive assessment of genetic variations that make use of decision analyses. The decision analyses sometimes include segmentation analyses and/or odds ratio analyses.
    Type: Grant
    Filed: May 23, 2014
    Date of Patent: June 30, 2020
    Assignee: Sequenom, Inc.
    Inventors: Chen Zhao, Zeljko Dzakula, Cosmin Deciu, Sung Kyun Kim, Amin R. Mazloom, Gregory Hannum, Mathias Ehrich
  • Patent number: 10697007
    Abstract: The methods described herein, referred to as PCR-Activated Sorting (PAS), allow nucleic acids contained in biological systems to be sorted based on their sequence as detected with nucleic acid amplification techniques, e.g., PCR. The nucleic acids can be free floating or contained within living or nonliving structures, including particles, viruses, and cells. The nucleic acids can include, e.g., DNA or RNA. Systems and devices for use in practicing methods of the invention are also provided.
    Type: Grant
    Filed: June 25, 2015
    Date of Patent: June 30, 2020
    Assignee: The Regents of the University of California
    Inventors: Adam R. Abate, Dennis Jay Eastburn, Adam R. Sciambi, Shaun Lim
  • Patent number: 10688221
    Abstract: This invention relates to methods for decellularising human liver tissue to produce human hepatic extracellular matrix (ECM) scaffolds, for example for use in therapy or disease modelling. The methods involve mechanically damaging cells in the tissue, for example by freeze thaw, and then subjecting the liver tissue to multiple cycles of osmotic stress, detergent treatment and protease and/or DNAase treatment to produce a decellularised human ECM scaffold.
    Type: Grant
    Filed: June 2, 2015
    Date of Patent: June 23, 2020
    Assignee: UCL BUSINESS PLC
    Inventors: Giuseppe Mazza, Massimo Malago, Paolo de Coppi, Massimo Pinzani
  • Patent number: 10676742
    Abstract: This invention relates to compounds, compositions, and methods useful for reducing transth:yretin (TTR) target RNA and protein levels via use of dsRNAs, e.g., Dicer substrate siRNA (DsiRNA) agents.
    Type: Grant
    Filed: October 23, 2018
    Date of Patent: June 9, 2020
    Assignee: Dicerna Pharmaceuticals, Inc.
    Inventors: Bob D. Brown, Henryk T. Dudek
  • Patent number: 10656021
    Abstract: A reflective detection device, includes: oscillation element oscillating a terahertz wave; exit part from which the terahertz wave exits; incident part to which the terahertz wave reflected from a detection target is incident; and detection element detecting the terahertz wave incident to the incident part, wherein the exit part and the incident part are disposed on one side in first direction and are spaced apart from each other in second direction with respect to the detection target, the terahertz wave exiting from the exit part travels to propagate from the exit part toward the incident part in the second direction along a direction toward the detection object in the first direction, and the terahertz wave incident to the incident part travels to propagate from the exit part toward the incident part in the second direction along a direction away from the detection target in the first direction.
    Type: Grant
    Filed: June 8, 2017
    Date of Patent: May 19, 2020
    Assignee: ROHM CO., LTD.
    Inventors: Hideyuki Kawakami, Jae-Young Kim, Toshikazu Mukai, Kazuisao Tsuruda
  • Patent number: 10633691
    Abstract: Method for studying constituents of individual molecular complexes by labelling the molecules belonging to the same complex with at least one set of molecular constructs, wherein each set member includes a Unique Identifying Sequence (UIS), which is a nucleic acid sequence unique for each set member, and at least one Common Tag Sequence (CTS), which is a nucleic acid sequence common to all set members, by: attaching the molecular construct to the complex by ligating or hybridizing the molecular construct to a nucleic acid molecule of the complex, or ligating or hybridizing the tag to a nucleic acid linked to an affinity binder that binds specifically to a constituent of the complex; labelling the molecules belonging to the same complex using the molecular construct tags as primers or templates in a nucleic acid polymerization reaction; and analyzing the composition of the complex by analyzing combinations of UISs and CTSs.
    Type: Grant
    Filed: September 30, 2014
    Date of Patent: April 28, 2020
    Inventor: Di Wu
  • Patent number: 10633647
    Abstract: A method for nucleic acid isolation comprising: receiving a binding moiety solution within a process chamber; mixing the binding moiety solution with a biological sample, within the process chamber, in order to produce a moiety-sample mixture; incubating the moiety-sample mixture during a time window, thereby producing a solution comprising a set of moiety-bound nucleic acid particles and a waste volume; separating the set of moiety-bound nucleic acid particles from the waste volume; washing the set of moiety-bound nucleic acid particles; and releasing a nucleic acid sample from the set of moiety-bound nucleic acid particles. The method preferably utilizes a binding moiety comprising at least one of poly(allylamine) and polypropylenimine tetramine dendrimer, both of which reversibly bind and unbind to nucleic acids based upon environmental pH.
    Type: Grant
    Filed: November 29, 2016
    Date of Patent: April 28, 2020
    Assignee: NeuMoDx Molecular, Inc.
    Inventors: Sundaresh Brahmasandra, Michelle Mastronardi, Elizabeth Craig, Maureen Carey
  • Patent number: 10627407
    Abstract: The presently disclosed subject matter provides methods for analyzing complex mixtures with ultra high resolution mass spectrometry. In particular, the presently disclosed subject matter provides methods for identifying and monitoring the presence of a compound within a complex mixture. In certain embodiments, the method includes providing a sample of the complex mixture; performing mass spectrometry on the sample of the complex mixture to obtain a mass spectrum; and identifying one or more peaks from the mass spectrum corresponding to the compound. In certain embodiments, the sample is obtained during production of the complex mixture, e.g., a pet food product. In certain embodiments, the sample is prepared by a single alcohol/water extraction step.
    Type: Grant
    Filed: March 11, 2016
    Date of Patent: April 21, 2020
    Assignee: MARS, INCORPORATED
    Inventors: James W. Marshall, Andrew J. Taylor
  • Patent number: 10611847
    Abstract: Disclosed are an ABCG2 monoclonal antibody having the effects for resisting against tumors and for reversing drug resistance of the tumors, and uses thereof in novel tumor resisting drugs. Also disclosed is a new antigen sequence, used for inducing generation of a monoclonal antibody or a polyclonal antibody for the ABCG2. In addition, disclosed is a hybridoma for generating the ABCG2 monoclonal antibody having the effects for resisting against tumors and for reversing drug resistance of the tumors.
    Type: Grant
    Filed: September 13, 2017
    Date of Patent: April 7, 2020
    Assignee: PEKING UNIVERSITY
    Inventor: Hongying Zhen
  • Patent number: 10585024
    Abstract: An apparatus for extracting a material from a liquid includes a concentration stage having a tangential flow filter, a first path from the tangential flow filter, and a second path from the tangential flow filter. Under this configuration, the concentration stage accepts an initial liquid volume. A first liquid not having material collected by the tangential flow filter is passed along the first path, and concentrated liquid having material therein, which is entrapped by the filter, is directed to the second path. The apparatus also includes an aerosolizing stage coupled to the concentration stage that converts the concentrated liquid into an aerosol and a drying stage that dries the aerosol such that material extracted from the aerosol onto a material substrate.
    Type: Grant
    Filed: August 20, 2019
    Date of Patent: March 10, 2020
    Assignee: BATTELLE MEMORIAL INSTITUTE
    Inventors: Andrew P. Bartko, Kurt Blohm, Ryan W. Daly, Aaron M. Deal, Scott M. Gibbons, Susana Lira-Gonzalez, Benjamin J. Richetti, Nathan Roehr, Theodore J. Ronningen, Christopher Scheitlin
  • Patent number: 10572811
    Abstract: Methods and systems for determining event probabilities and anomalous events are provided. In one implementation, a method includes: receiving source data, where the source data is configured as a plurality of events with associated timestamps; searching the source data, where the searching provides a search result including N events from the plurality of events, where N is an integer greater than one, where each event of the N events includes a plurality of field values, where at least one event of the N events can include one or more categorical field values and one or more numerical field values; and for an event of the N events, determining a probability of occurrence for each field value of the plurality of field values; and using probabilities determined for the plurality of field values, determining a probability of occurrence for the event.
    Type: Grant
    Filed: January 29, 2015
    Date of Patent: February 25, 2020
    Assignee: SPLUNK INC.
    Inventors: Nghi Nguyen, Jacob Leverich, Adam Oliner
  • Patent number: 10562997
    Abstract: A method of purifying a biological composition includes: disposing loose cationic ligand-functionalized staple fibers and a biological composition within a mixing volume of a vessel; agitating the biological composition and the loose cationic ligand-functionalized staple fibers while they are in intimate contact with each other within the mixing volume to provide modified fibers and a purified biological composition; and separating at least a portion of the purified biological composition from the modified fibers and any loose cationic ligand-functionalized staple fibers with which it is in contact. The loose cationic ligand-functionalized staple fibers have a modified surface layer comprising a grafted acrylic polymer comprising 10 to 100 percent by weight of a cationically-ionizable monomer unit. An article for purifying a biological composition includes: a vessel having a mixing volume disposed therein; and the loose cationic ligand-functionalized staple fibers disposed within the mixing volume.
    Type: Grant
    Filed: March 17, 2016
    Date of Patent: February 18, 2020
    Assignee: 3M Innovative Properties Company
    Inventors: Andrew W. Vail, Jerald K. Rasmussen, John J. Schmidt, Ali E. Ozcam, Jonathan F. Hester, Gregory M. Jellum
  • Patent number: 10544166
    Abstract: The present specification relates to a novel compound, and a color conversion film, a backlight unit and a display apparatus including the same.
    Type: Grant
    Filed: October 10, 2016
    Date of Patent: January 28, 2020
    Assignee: LG CHEM, LTD.
    Inventors: Hoyong Lee, Duy Hieu Le, Jiyeon Sung
  • Patent number: 10532330
    Abstract: Filtration membrane comprising polymeric nanofibers and/or microfibers attaching dendrimer component presenting reactive sites selective for chemicals to be filtered, and related nanofibers and microfibers, composite materials, compositions, methods and system.
    Type: Grant
    Filed: April 25, 2017
    Date of Patent: January 14, 2020
    Assignees: CALIFORNIA INSTITUTE OF TECHNOLOGY, KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY
    Inventors: Mamadou S. Diallo, William A. Goddard, III, Seong-Jik Park, Manki Cho
  • Patent number: 10527626
    Abstract: Provided herein, among other aspects, are methods and apparatuses for analyzing particles in a sample. In some aspects, the particles can be analytes, cells, nucleic acids, or proteins and contacted with a tag, partitioned into aliquots, detected by a ranking device, and isolated. The methods and apparatuses provided herein may include a microfluidic chip. In some aspects, the methods and apparatuses may be used to quantify rare particles in a sample, such as cancer cells and other rare cells for disease diagnosis, prognosis, or treatment.
    Type: Grant
    Filed: July 1, 2014
    Date of Patent: January 7, 2020
    Assignee: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION
    Inventors: Daniel T. Chiu, Mengxia Zhao, Wyatt Nelson, Perry G. Schiro
  • Patent number: 10508285
    Abstract: The present invention relates to a method for identifying a polynucleotide encoding a polypeptide which confers herbicide tolerance to a plant by measuring photosynthetic quantum yield wherein an increase in the electron transport rate of the samples of the transformed plant as compared to the sample of the control plant is indicative for a herbicide tolerance conferring activity of said candidate polypeptide.
    Type: Grant
    Filed: November 20, 2015
    Date of Patent: December 17, 2019
    Assignee: BASF SE
    Inventors: Stefan Tresch, Raphael Aponte, Jens Lerchl, Johannes Hutzler
  • Patent number: 10504614
    Abstract: Computerized analysis methods and systems to implement the computerized analysis methods are disclosed herein. Specifically, the present disclosure relates to systems and methods for determining an unknown characteristic of a sample.
    Type: Grant
    Filed: October 7, 2014
    Date of Patent: December 10, 2019
    Assignees: RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY, TRUSTEES OF BOSTON UNIVERSITY, MASSACHUSETTS INSTITUTE OF TECHNOLOGY
    Inventors: Catherine M. Grgicak, Desmond S. Lun, Muriel Medard, Harish Swaminathan
  • Patent number: 10480032
    Abstract: The present disclosure relates to a novel use of EI24, and provides a novel use of EI24 involved in the improvement of EGFR-TKI drug resistance by inhibiting IGF-1R signaling. According to the present disclosure, the presence or absence of resistance to anti-cancer drugs may be determined by detecting an expression level of EI24. In addition, resistance to anti-cancer drugs may be inhibited, delayed, or improved using EI24 or an activating agent thereof. Moreover, by regulating a pathway of EI24-mediated resistance to anti-cancer drugs, the efficacy of existing anti-cancer drugs may be enhanced, and resistance to anti-cancer drugs may be inhibited, delayed, or improved.
    Type: Grant
    Filed: March 14, 2017
    Date of Patent: November 19, 2019
    Assignee: University-Industry Foundation, Yonsei University
    Inventors: Han Woong Lee, Jung-Min Choi, Byoung Chul Cho, Yu-Ra Choi, Ji-Young Jang, Hye Ryun Kim
  • Patent number: 10465232
    Abstract: Methods for quantifying the efficiency of nucleic acid extraction from a sample comprising a mixture of species are provided. In some embodiments, the method comprises adding an initial amount of one or more spike-ins to the sample, extracting nucleic acids from the sample, quantifying the amount of the one or more spike-ins in the extracted nucleic acid sample, and comparing the initial amount of the one or more spike-ins to the quantified amount of the one or more spike-ins.
    Type: Grant
    Filed: October 7, 2016
    Date of Patent: November 5, 2019
    Assignee: Trace Genomics, Inc.
    Inventors: Diane Wu, Poornima Parameswaran, Scott Hickey
  • Patent number: 10457978
    Abstract: The invention concerns methods for detecting a nucleic acid of interest in a solution comprising (a) contacting a solution suspected of containing the nucleic acid of interest with a PNA capture probe and a PNA reporter probe; wherein (i) the PNA capture probe comprises at least two trans-cyclopentanes; (ii) the PNA reporter probe comprises at least six biotin groups; (iii) the PNA capture probe bound to a surface; and (iv) the PNA capture probe and the PNA reporter probe each comprise a nucleobase sequence that is complementary to different non-overlapping portions of the nucleic acid of interest; (b) detecting the presence of the PNA capture probe and the PNA reporter probe bound to the surface; wherein the nucleic acid of interest is detected when 1-1000 molecules of the nucleic acid of interest are present in the solution being tested.
    Type: Grant
    Filed: August 16, 2013
    Date of Patent: October 29, 2019
    Assignee: The United States of America, as represented by the Secretary, Department of Health and Human Services
    Inventors: Daniel H. Appella, Christopher Micklitsch, Bereket Yemane, Chao Zhao
  • Patent number: 10434804
    Abstract: The present teachings relate to various embodiments of a gas enclosure system that can have various components comprising a particle control system that can provide a low-particle zone proximal to a substrate. Various components of a particle control system can include a gas circulation and filtration system, a low-particle-generating motion system for moving a printhead assembly relative to a substrate, a service bundle housing exhaust system, and a printhead assembly exhaust system. In addition to maintaining substantially low levels for each species of various reactive species, including various reactive atmospheric gases, such as water vapor and oxygen, for various embodiments of a gas enclosure system that have a particle control system, an on-substrate particle specification can be readily met. Accordingly, processing of various substrates in an inert, low-particle gas environment according to systems and methods of the present teachings can have substantially lower manufacturing defects.
    Type: Grant
    Filed: May 12, 2014
    Date of Patent: October 8, 2019
    Assignee: Kateeva, Inc.
    Inventors: Justin Mauck, Alexander Sou-Kang Ko, Eliyahu Vronsky, Shandon Alderson, Alexey Stepanov
  • Patent number: 10436784
    Abstract: The present invention relates to methods and kits to assess an absorbed dose of ionizing radiation and/or the severity of tissue injury from radiation in a patient. The invention also relates to algorithms used to calculate an absorbed dose of radiation based on biomarker measurements of a plurality of biomarkers that are altered relative to a normal control in the event of radiation exposure.
    Type: Grant
    Filed: September 15, 2015
    Date of Patent: October 8, 2019
    Assignees: Meso Scale Technologies, LLC, The Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc.
    Inventors: William F Blakely, Eli N. Glezer, John Kenten, Sudeep Kumar, Anu Mathew, Natalia I. Ossetrova, George Sigal
  • Patent number: 10385394
    Abstract: The present invention relates to processes for characterizing and screening for the existence or predisposition to X-linked disorders associated with changes in X-chromosome inactivation. The present invention also relates to processes of reducing a disease phenotype associated with an X-linked disorder in a female subject.
    Type: Grant
    Filed: March 17, 2014
    Date of Patent: August 20, 2019
    Assignee: The Translational Genomics Research Institute
    Inventors: Szabolcs Szelinger, David W. Craig, Matthew Huentelman, Vinodh Narayanan
  • Patent number: 10260087
    Abstract: The present invention relates to methods for the identification of methylated cytosines in a population of double stranded DNA molecules. The invention also relates to adapters and kits for synthesizing said adapters as well as to double stranded DNA libraries obtained by the methods of the invention.
    Type: Grant
    Filed: January 7, 2015
    Date of Patent: April 16, 2019
    Assignees: Fundació Privada Institut De Medicina Predictiva I Personalitzada Del Cáncer
    Inventors: Llorenç Coll Mulet, Miguel A. Peinado Morales
  • Patent number: 10260103
    Abstract: The present invention provides dignostic assays for identifying thyroid cancer in a biological sample, including a fine needle aspirate, as well as related compositions and kits useful in practicing the methods of the invention.
    Type: Grant
    Filed: November 26, 2013
    Date of Patent: April 16, 2019
    Assignee: PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE
    Inventors: Hernan Eugenio Gonzalez Diaz, Sergio Vargas Salas, Jose Rodrigo Waldemar Martinez Solis
  • Patent number: 10240214
    Abstract: The present disclosure provides compositions (i.e., amplification primers and probes), methods, and kits that are particularly useful for detecting and/or quantifying nucleic acids present in a sample, such as those derived from HIV or a lentiviral vector.
    Type: Grant
    Filed: November 13, 2017
    Date of Patent: March 26, 2019
    Assignees: Calimmune, Inc., Calimmune Australia Pty Ltd, St. Vincent's Hospital Sydney Limited, Newsouth Innovations Pty Limited
    Inventors: Anthony Dominic Kelleher, Kazuo Suzuki, Geoffrey Phillip Symonds
  • Patent number: 10221381
    Abstract: Systems, devices, and methods for generating, culturing, and using magnetically-controlled three-dimensional (3D) tissues are described. A magnetically-enabled transwell system for immobilizing and supplying perfusion to a 3D mini-tissue includes a cell culture vessel with at least a first chamber and a second chamber, the mini-tissue being disposed in the first chamber, and a perfusion mechanism defining an elongate perfusion channel with a proximal end in the first chamber and a distal end in fluid communication with the second chamber, with a magnetic element configured to immobilize a magnetically-controlled 3D mini-tissue such that the proximal end is in fluid communication with basolateral space of the immobilized mini-tissue.
    Type: Grant
    Filed: June 30, 2015
    Date of Patent: March 5, 2019
    Assignee: Dana-Faber Cancer Institute, Inc.
    Inventors: Eugen Dhimolea, Constantine Mitsiades
  • Patent number: 10160974
    Abstract: Recombinant nucleic acid constructs for expression of heterologous cytokines or chemokines in C. albicans, and genetically modified C. albicans strains comprising the recombinant nucleic acid constructs, are described. The recombinant nucleic acid molecules include a C. albicans gene promoter sequence, a nucleic acid sequence encoding a C. albicans secreted protein signal sequence and a heterologous open reading frame (ORF) of a cytokine or chemokine gene. Also described are a method of expressing a heterologous cytokine or chemokine protein in a subject, and a method of treating or inhibiting the development of candidiasis in a subject. These methods include administering a genetically modified C. albicans containing a recombinant nucleic acid construct for expression of a heterologous cytokine or chemokine. Exemplary cytokines or chemokines include IL8, IL17A, CXCL1, CXCL2 and CXCL5.
    Type: Grant
    Filed: February 10, 2015
    Date of Patent: December 25, 2018
    Assignees: University of Pittsburgh—Of the Commonwealth System of Higher Education, Carnegie Mellon University
    Inventors: Sarah L. Gaffen, Mandy J. McGeachy, Anna R. Huppler, Aaron P. Mitchell
  • Patent number: 10150965
    Abstract: This invention relates to compounds, compositions, and methods useful for reducing transthyretin (TTR) target RNA and protein levels via use of dsRNAs, e.g., Dicer substrate siRNA (DsiRNA) agents.
    Type: Grant
    Filed: December 5, 2014
    Date of Patent: December 11, 2018
    Assignee: Dicerna Pharmaceuticals, Inc.
    Inventors: Bob D. Brown, Henryk T. Dudek
  • Patent number: 10138489
    Abstract: The invention provides genetically modified cyanobacterial cells that are capable of utilizing phosphite as a primary phosphorus source, and can out-compete contaminant organisms for certain forms of phosphorus more effectively.
    Type: Grant
    Filed: October 12, 2017
    Date of Patent: November 27, 2018
    Assignee: Algenol Biotech LLC
    Inventors: Paul Gordon Roessler, Charles Ryan Budinoff, Songhua Zhu, Kui Wang, Heike Enke, Christian Weissert, Frank Jochem, Ming-De Deng
  • Patent number: 10127353
    Abstract: According to various embodiments, aspects of the invention provide a highly efficient meta-analysis infrastructure for performing research queries across a large number of studies and experiments from diverse sequencing technologies as well as different biological and chemical assays, data types and organisms, as well as systems to build and add to such an infrastructure. The methods, systems and apparatuses described enable combining orthogonal types of data and available public knowledge to elucidate mechanisms governing normal development, disease progression, as well as susceptibility of individuals to disease or response to drug treatments.
    Type: Grant
    Filed: March 6, 2017
    Date of Patent: November 13, 2018
    Assignee: Nextbio
    Inventors: Ilya Kupershmidt, Qiaojuan Jane Su
  • Patent number: 10087456
    Abstract: Polynucleotide sequences encoding diacylglycerol acyltransferase genes and the use of these acyltransferases for increased seed storage lipid production and altered fatty acid profiles in oilseed plants are disclosed. Transgenic soybean seed having increased total fatty acid content of at least 20% and altered fatty acids when compared to the total fatty acid content of non-transgenic, null segregant soybean seed are described. Methods for increasing the total fatty acid content of a soybean seed by at least 20% include steps of transformation and selection.
    Type: Grant
    Filed: January 10, 2017
    Date of Patent: October 2, 2018
    Assignee: E I DU PONT DE NEMOURS AND COMPANY
    Inventors: Knut Meyer, Kevin L Stecca
  • Patent number: 10060912
    Abstract: Methods for proteomic screening on random protein-bead arrays by mass spec is described. Photocleavable mass tags are utilized to code a protein library (bait molecules) displayed on beads randomly arrayed in an array substrate. A library of probes (prey) can be mixed with the protein-bead array to query the array. Because mass spec can detect multiple mass tags, it is possible to rapidly identify all of the interactions resulting from this mixing.
    Type: Grant
    Filed: October 3, 2016
    Date of Patent: August 28, 2018
    Assignee: AmberGen, Inc.
    Inventors: Mark J Lim, Vladislav B Bergo, Kenneth J Rothschild
  • Patent number: 10051831
    Abstract: Methods of producing hybrid Agaricus bisporus mushrooms strains derived or descended from at least one wild mushroom strain and having the specified traits of either greatly diminished sporulation or an absence of sporulation, and of obtaining postmeiotic offspring of nonsporlating basidiomycete fungi including Agaricus bisporus, are disclosed.
    Type: Grant
    Filed: July 16, 2010
    Date of Patent: August 21, 2018
    Assignee: SYLVAN AMERICA, INC.
    Inventors: Richard W. Kerrigan, Anthony J. Velcko, Jr., Mark C. Spear, Mark P. Wach