Involving A Nucleic Acid Encoding An Enzyme Patents (Class 435/6.18)
  • Patent number: 11371048
    Abstract: The present invention provides expression vectors for use in an inducible coexpression system, capable of controlled induction of expression of each gene product.
    Type: Grant
    Filed: September 11, 2016
    Date of Patent: June 28, 2022
    Assignee: ABSCI LLC
    Inventors: Sean McClain, Mark Valasek, Philip Barish, Jeremy Minshull
  • Patent number: 11332747
    Abstract: Bispecific aptamers having a first end that specifically binds to a first tumor specific marker, tumor antigen, or viral protein and a second end that specifically binds to a second tumor specific marker, tumor antigen, or viral protein are provide. The bispecific aptamers can be used to treat cancer or virally infected cells. Generally, the bispecific aptamers bind to two surface proteins, preferably different proteins, on the same cell. In a preferred embodiment the bispecific aptamers bind to two different tumor markers, tumor antigens, tumor specific proteins and combinations thereof.
    Type: Grant
    Filed: February 20, 2018
    Date of Patent: May 17, 2022
    Assignee: AUGUSTA UNIVERSITY RESEARCH INSTITUTE, INC.
    Inventor: Hongyan Liu
  • Patent number: 11332779
    Abstract: Methods are provided for nucleic acid analysis wherein a target nucleic acid is mixed with a dsDNA binding dye to form a mixture. Optionally, an unlabeled probe is included in the mixture. A melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.
    Type: Grant
    Filed: April 9, 2020
    Date of Patent: May 17, 2022
    Assignees: BIOFIRE DEFENSE, LLC, UNIVERSITY OF UTAH RESEARCH FOUNDATION
    Inventors: Luming Zhou, Carl T. Wittwer, Philip Seth Bernard, Virginie Dujols
  • Patent number: 11326215
    Abstract: The present invention provides novel methods of diagnosing and determining treatment strategies for Lyme disease and other tick-borne illnesses.
    Type: Grant
    Filed: November 18, 2019
    Date of Patent: May 10, 2022
    Assignee: RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY
    Inventors: Nikhat Parveen, Salvatore A. E. Marras
  • Patent number: 11326193
    Abstract: The present invention relates to methods of providing a biomass residuum and compositions thereof. In particular examples, the biomass residuum includes one or more high value amino acids, even after removal of mixed alcohol components. In particular, the methods include implementing pre-treatment conditions and employing fermentation conditions including modified organisms.
    Type: Grant
    Filed: April 18, 2019
    Date of Patent: May 10, 2022
    Assignee: National Technology & Engineering Solutions of Sandia, LLC
    Inventors: Ryan Wesley Davis, Fang Liu
  • Patent number: 11321831
    Abstract: Systems and methods are provided for provided for automatic evaluation of a human embryo. An image of the embryo is obtained and provided to a neural network to generate a plurality of values representing the morphology of the embryo. The plurality of values representing the morphology of the embryo are evaluated at an expert system to provide an output class representing one of a current quality of the embryo, a future quality of the embryo, a likelihood that implantation of the embryo will be successful, and a likelihood that implantation of the embryo will result in a live birth.
    Type: Grant
    Filed: October 1, 2018
    Date of Patent: May 3, 2022
    Assignees: THE BRIGHAM AND WOMEN'S HOSPITAL, INC., THE GENERAL HOSPITAL CORPORATION
    Inventors: Hadi Shafiee, Charles Bormann, Manoj Kumar Kanakasabapathy, Prudhvi Thirumalaraju
  • Patent number: 11295546
    Abstract: Method of tracking a plurality of objects comprising: focusing an image of the objects on an imaging element using an optical system; capturing the image of the objects using an imaging element comprising a plurality of pixels; measuring at least one characteristic of the objects from the captured image using an image processor; wherein the field of view is set be the widest field of view for which the image processor is able to measure the at least one characteristic.
    Type: Grant
    Filed: March 7, 2019
    Date of Patent: April 5, 2022
    Assignees: Cambridge Enterprise Limited, Wren Therapeutics Limited
    Inventors: Michele Perni, Quentin Alexis Eric Peter, Pavan Kumar Challa, Tadas Kartanas, Pietro Sormanni, Julius Bier Kirkegaard, Michele Vendruscolo, Christopher Martin Dobson, Tuomas Pertti Jonathan Knowles
  • Patent number: 11286526
    Abstract: There is disclosed a composition of an aqueous solution comprising, consisting or consisting essentially of a flap endonuclease, a bulking agent and an organic buffer, wherein the aqueous solution has an inorganic salt concentration of 5 mM or less and wherein the composition is substantially free of glycerol.
    Type: Grant
    Filed: May 18, 2018
    Date of Patent: March 29, 2022
    Assignee: Gen-Probe Incorporated
    Inventors: Patrick Peterson, Tony Luu, Matthias Jost
  • Patent number: 11279969
    Abstract: The present disclosure relates to chemical compositions, kits, and apparatuses and methods for using these compositions, kits and apparatuses in various assays.
    Type: Grant
    Filed: September 4, 2019
    Date of Patent: March 22, 2022
    Assignee: NanoString Technologies, Inc.
    Inventors: Dwayne L. Dunaway, Elizabeth A. Manrao, Joseph M. Beechem, Rustem Khafizov, Sanghamithra Korukonda, Yi Deng, Dae Kim, Mark Gregory, Margaret Hoang
  • Patent number: 11268134
    Abstract: A sensor apparatus for testing a biological sample, a system making use thereof and a method for testing a biological sample with the sensor apparatus and system.
    Type: Grant
    Filed: September 26, 2018
    Date of Patent: March 8, 2022
    Assignee: BOEHRINGER INGELHEIM VETMEDICA GMBH
    Inventors: Christoph Weber, Matthias Griessner, Nele Jenne
  • Patent number: 11255837
    Abstract: Devices and methods for recording dynamics of cellular and/or biochemical processes, including a device including one or more dispersive elements configured to receive a pulsed laser beam with a spectrum of different wavelengths and disperse the spectrum of the pulsed laser beam; and one or more first elements configured to receive the dispersed spectrum of the pulsed laser beam, and generate a multiphoton excitation area in a biological sample by re-overlapping in time and space the dispersed spectrum of the pulsed laser beam on an area in the biological sample, wherein the device is configured to record at high speed changes of cellular and biochemical processes of a population of cells of the biological sample based on generation of the multiphoton excitation area in the biological sample.
    Type: Grant
    Filed: May 24, 2019
    Date of Patent: February 22, 2022
    Assignee: UNIVERSITY OF VIENNA
    Inventors: Alipasha Vaziri, Robert Prevedel
  • Patent number: 11242552
    Abstract: A nucleic acid integrated detection method and detection reagent tube are provided, separating a lysis solution, a cleaning solution and a reaction solution in a detection reagent tube by providing a plurality of separation plugs in an over-under arrangement and disposing a hydrophobic layer in liquid or solid phase on each separation plug; adding a sample into the lysis solution; extracting nucleic acid in the sample using magnetic nanobeads; and then driving the magnetic nanobeads carrying the nucleic acid to sequentially pass through each hydrophobic layer along a magnetic bead channel and into the cleaning solution and the reaction solution to realize a cleaning and amplification for the nucleic acid, and finally, detecting the nucleic acid of the sample by an external device using an optical detection method, thus realizing a plurality of steps of nucleic acid extraction, cleaning and amplification reactions in the same detection reagent tube.
    Type: Grant
    Filed: February 1, 2019
    Date of Patent: February 8, 2022
    Assignee: USTAR Biotechnologies (Hangzhou) Ltd.
    Inventors: Qimin You, Lin Hu, Chen Qi, Junwei Yu, Zhujun Yu, Sha Wang, Rongyu Jin, Daisang Wang, Sisi Chen, Junli He, Jing Chen, Huanxin Rao, Yanqiong Zhou, Fan Yang
  • Patent number: 11207380
    Abstract: The present invention provides compounds, compositions, and methods for detecting, diagnosing and treating cancers such as glioblastoma multiforme.
    Type: Grant
    Filed: April 19, 2018
    Date of Patent: December 28, 2021
    Assignee: Wake Forest University Health Sciences
    Inventors: Waldemar Debinski, Jill Wykosky, Denise Mazess Herpai
  • Patent number: 11208671
    Abstract: A recombinant cell and a method of producing itaconic acid using such recombinant cell. The recombinant cell is of the genus Methylorubrum and includes a first polynucleotide sequence as defined in SEQ ID NO: 1 or a homologue thereof operably linked to a regulatory sequence.
    Type: Grant
    Filed: July 12, 2019
    Date of Patent: December 28, 2021
    Assignee: City University of Hong Kong
    Inventors: Chee Kent Lim, Patrick Kwan Hon Lee
  • Patent number: 11183273
    Abstract: A method for simulating a biochemical environment utilizes a heterogeneous process model, which evaluates both a flux balance analysis and one or more detailed models each on a different but overlapping sets of molecules in the biochemical environment. The heterogeneous process model evaluates the flux balance analysis based on a stoichiometric matrix, a flux vector including initial internal exchange flux values, and an objective function. The heterogeneous process model evaluates the one or more detailed models based on an initial set of molecule concentrations and a plurality of detailed model parameters. The results are then used to update the exchange fluxes and molecules concentrations. The process is repeated thereby integrating the results of the flux balance analysis with the one or more detailed models.
    Type: Grant
    Filed: September 1, 2017
    Date of Patent: November 23, 2021
    Assignee: X Development LLC
    Inventors: Jason Thompson, Frank Russo
  • Patent number: 11164659
    Abstract: Disclosed herein are methods and systems for identifying targets for distinguishing cell types. In some embodiments, the method comprises: hierarchically clustering expression profiles of cells to generate a dendrogram with each leaf representing the expression profile of a different individual cell. The dendrogram can be pruned by eliminating invalid splits (and their children). The remaining leaves in the dendrogram can be merged, independent of their locations in the dendrogram, based on their distances to one another to generate clusters of expression profiles. The method can identify targets for distinguishing cell types based on targets that are expressed differentially in the clusters.
    Type: Grant
    Filed: November 7, 2017
    Date of Patent: November 2, 2021
    Assignee: Becton, Dickinson and Company
    Inventors: Jue Fan, Jesse Zhang, Jing Hu
  • Patent number: 11155860
    Abstract: The invention relates to a method of characterising a target polynucleotide using a single-stranded binding protein (SSB). The SSB is either an SSB comprising a carboxy-terminal (C-terminal) region which does not have a net negative charge or a modified SSB comprising one or more modifications in its C-terminal region which decreases the net negative charge of the C-terminal region.
    Type: Grant
    Filed: July 18, 2013
    Date of Patent: October 26, 2021
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: James White, Ruth Moysey, Mihaela Misca
  • Patent number: 11119096
    Abstract: A kit of parts and methods for determining the presence and quantity of one or more therapeutic monoclonal antibody drugs and/or therapeutic monoclonal antibody inhibitor drug antibodies in a biological sample, the method including providing a reaction liquid comprising the sample, a first conjugate with the target of the therapeutic monoclonal antibody and a first conjugated moiety and a second conjugate with the target of the therapeutic monoclonal antibody and a second conjugated moiety, followed by detecting the change in spectrophotometric signal when the complex between the therapeutic monoclonal antibody drug, the first conjugate and a the second conjugate forms.
    Type: Grant
    Filed: July 3, 2017
    Date of Patent: September 14, 2021
    Assignee: W. Health L.P.
    Inventors: Peter Warthoe, Henrik Rindel Gudbergsen
  • Patent number: 11091758
    Abstract: The disclosure provides for methods to form compact cross-linked polynucleotide/protein structures that can then be labeled using a barcoded oligonucleotide array in order to reconstruct physical linkage and/or genomic proximity (and phase) of polynucleotide fragments.
    Type: Grant
    Filed: December 11, 2014
    Date of Patent: August 17, 2021
    Assignee: The Regents of the University of California
    Inventors: Daniel Rokhsar, Richard E. Green, Jr.
  • Patent number: 11085931
    Abstract: The present invention relates to a kit of parts and methods for determining the presence and quantity of one or more TNF-? inhibitor drugs and/or anti-TNF-? inhibitor drug antibodies in one or more biological samples each comprising less than 200 ?l, the method comprising the steps of providing a reaction liquid comprising the sample, a first TNF-? conjugate comprising TNF-? and a first conjugated moiety and a second TNF-? conjugate comprising TNF-? and a second conjugated moiety, said second moiety being capable of generating or ameliorating a detectable signal in the presence of a molecular complex comprising a TNF-? inhibitor, followed by detecting the change in signal when the complex between the TNF-? inhibitor drug, the first TNF-? conjugate and a the second TNF-? conjugate forms.
    Type: Grant
    Filed: January 11, 2016
    Date of Patent: August 10, 2021
    Assignee: W. HEALTH L.P.
    Inventor: Peter Warthoe
  • Patent number: 11079389
    Abstract: The present invention provides a method for identifying a synthetic classifier including contacting at least a first and second samples derived from different groups of a cohort with a first plurality of peptides. The first plurality of peptides includes a first subset of peptides defining at least one naturally occurring amino acid sequence, and a second subset of peptides defining a plurality of variants of the first subset of peptides. The plurality of variants includes, for each one of the first subset of peptides, a variant peptide having at least one of a substitution, a deletion, an insertion, an extension, and a modification. The method further includes selecting at least one of the plurality of variants from the second subset of peptides, and defining a synthetic classifier including the at least one of the plurality of variants that distinguishes between samples derived from the first and second cohorts.
    Type: Grant
    Filed: June 3, 2020
    Date of Patent: August 3, 2021
    Assignee: Roche Sequencing Solutions, Inc.
    Inventors: Hanying Li, Ken Lo, Jigar Patel
  • Patent number: 11058767
    Abstract: The present disclosure relates to antisense oligonucleotides, which target CAMK2D mRNA in a cell, leading to reduced expression of CAMK2D protein. Reduction of CAMK2D protein expression is beneficial for the treatment of certain medical disorders, e.g., cardiovascular-related diseases or disorders.
    Type: Grant
    Filed: February 21, 2019
    Date of Patent: July 13, 2021
    Assignees: BRISTOL-MYERS SQUIBB COMPANY, ROCHE INNOVATION CENTER COPENHAGEN A/S
    Inventors: Richard E. Olson, Brian R. Anderson, Peter Hagedorn, Marianne Lerbech Jensen, Ivar M. McDonald, Stephen E. Mercer
  • Patent number: 11028380
    Abstract: The present invention provides a Cas9 platform to facilitate single-site nuclease gene editing precision within a human genome. For example, a Cas9 nuclease/DNA-targeting unit (Cas9-DTU) fusion protein precisely delivers a Cas9/sgRNA complex to a specific target site within the genome for subsequent sgRNA-dependent cleavage of an adjacent target sequence. Alternatively, attenuating Cas9 binding using mutations to the a protospacer adjacent motif (PAM) recognition domain makes Cas9 target site recognition dependent on the associated DTU, all while retaining Cas9's sgRNA-mediated DNA cleavage fidelity. Cas9-DTU fusion proteins have improved target site binding precision, greater nuclease activity, and a broader sequence targeting range than standard Cas9 systems. Existing Cas9 or sgRNA variants (e.g., truncated sgRNAs (tru-gRNAs), nickases and FokI fusions) are compatible with these improvements to further reduce off-target cleavage.
    Type: Grant
    Filed: December 19, 2018
    Date of Patent: June 8, 2021
    Assignee: University of Massachusetts
    Inventors: Scot Andrew Wolfe, Mehmet Fatih Bolukbasi, Ankit Gupta, Erik J Sontheimer, Nadia Amrani
  • Patent number: 11027502
    Abstract: Techniques for forming a nanopore in a lipid bilayer are described herein. In one example, an agitation stimulus level such as an electrical agitation stimulus is applied to a lipid bilayer wherein the agitation stimulus level tends to facilitate the formation of nanopores in the lipid bilayer. In some embodiments, a change in an electrical property of the lipid bilayer resulting from the formation of the nanopore in the lipid bilayer is detected, and a nanopore has formed in the lipid bilayer is determined based on the detected change in the lipid bilayer electrical property.
    Type: Grant
    Filed: November 2, 2018
    Date of Patent: June 8, 2021
    Assignee: Roche Sequencing Solutions, Inc.
    Inventors: Roger J. A. Chen, Randy Davis
  • Patent number: 11008359
    Abstract: The invention provides a nucleotide or nucleoside having a base attached to a detectable label via a cleavable linker, characterised in that the cleavable linker contains a moiety selected from the group comprising: Formula (I) (wherein X is selected from the group comprising O, S, NH and NQ wherein Q is a C1-10 substituted or unsubstituted alkyl group, Y is selected from the group comprising O, S, NH and N(allyl), T is hydrogen or a C1-10 substituted or unsubstituted alkyl group and * indicates where the moiety is connected to the remainder of the nucleotide or nucleoside).
    Type: Grant
    Filed: June 30, 2020
    Date of Patent: May 18, 2021
    Assignee: Illumina Cambridge Limited
    Inventors: Xiaohai Liu, John Milton, Silke Ruediger, Xiaolin Wu
  • Patent number: 11001890
    Abstract: Methods are disclosed for detecting peripheral mitochondrial DNA damage and dysfunction. Methods are also disclosed that utilize blood samples to detect a neurodegenerative disease, such as Parkinson's disease, and determine the efficacy of therapy.
    Type: Grant
    Filed: September 8, 2016
    Date of Patent: May 11, 2021
    Assignee: University of Pittsburgh—Of the Commonwealth System of Higher Education
    Inventors: Laurie H. Andolina, John T. Greenamyre, Sruti Shiva
  • Patent number: 11000581
    Abstract: This invention relates to the delivery of heterologous peptides or proteins such as therapeutic peptides, therapeutic proteins or antigens to mucosal sites using vesicles derived from the outer membrane of commensal bacteria, recombinant bacteria capable of producing such vesicles, and methods for the production of such vesicles. The invention further relates to an inducible expression system for use in recombinant bacteria.
    Type: Grant
    Filed: April 28, 2017
    Date of Patent: May 11, 2021
    Assignees: QUADRAM INSTITUTE BIOSCIENCE, UEA ENTERPRISES LIMITED
    Inventors: Regis Gabriel Stentz, Simon Richard Carding
  • Patent number: 10988764
    Abstract: The present disclosure provides compositions and methods for regulating gene expression via RNA-mediated silencing. The present disclosure also provides compositions and methods to optimize the processing of a dsRNA molecule into small RNA duplexes. The present disclosure further provides compositions and methods to improve the efficiency of a dsRNA molecule in producing desired small RNAs and promoting the silencing of a gene of interest.
    Type: Grant
    Filed: June 22, 2015
    Date of Patent: April 27, 2021
    Assignee: Monsanto Technology LLC
    Inventors: Alberto Iandolino, Juan Pedro Sanchez
  • Patent number: 10982290
    Abstract: Disclosed is a method for detecting and quantifying gut flora derived from human faeces, comprising acquiring a solid support containing human faeces; optionally amplifying nucleic acid from the human faeces; and detecting and quantifying the presence of gut flora of interest. Also disclosed is a method for assessing whether a person has type-2 diabetes as well as a kit for detecting and quantifying gut flora from human faeces, comprising a solid support for collecting human faeces; and primer pairs for amplifying 16S rRNA sequence from bacteria of interest.
    Type: Grant
    Filed: July 23, 2015
    Date of Patent: April 20, 2021
    Assignee: Global Life Sciences Solutions Operations UK Ltd
    Inventors: Jeffrey Kenneth Horton, Peter James Tatnell, Matthew Sam Morrison, Alan Stuart Pierce
  • Patent number: 10961557
    Abstract: A system for identifying at least one species, strain or type of bacteria in a sample including at least one a labeled bacteriophage which binds selectively to the species, strain or type of bacteria, the at least one labeled bacteriophage comprising a label attached thereto, the system further comprising a detection system adapted to detect the labeled bacteriophage bound to the species of bacteria.
    Type: Grant
    Filed: December 19, 2019
    Date of Patent: March 30, 2021
    Assignees: University of Pittsburgh Of the Commonwealth System of Higher Education, Duquesne University of the Holy Spirit
    Inventors: John Alston Kellum, John D. Hempel, Robert Hugh Edgar, John Andrew Viator
  • Patent number: 10934598
    Abstract: Nucleic acid oligomers specific for human parvovirus genomic DNA are disclosed. An assay for amplifying and detecting human parvovirus genotypes 1, 2 and 3 nucleic acid in biological specimens is disclosed. Compositions for amplifying and detecting the presence of human parvovirus genotypes 1, 2 and 3 genomic DNA in human biological specimens are disclosed.
    Type: Grant
    Filed: June 20, 2018
    Date of Patent: March 2, 2021
    Assignee: GEN-PROBE INCORPORATED
    Inventor: James M. Carrick
  • Patent number: 10907198
    Abstract: The present invention provides assays systems and methods for detection of chromosomal abnormalities and status of single loci associated with monogenic or polygenic traits in a sample containing nucleic acids from a maternal and a fetal source.
    Type: Grant
    Filed: October 12, 2018
    Date of Patent: February 2, 2021
    Assignee: Ariosa Diagnostics, Inc.
    Inventors: Andrew Sparks, Arnold Oliphant, Jacob Zahn, Ken Song, John Stuelpnagel
  • Patent number: 10900079
    Abstract: Compositions and methods for highly specific nucleic acid probes and primers are provided. The probe system comprises a complement strand and a protector stand that form a partially double-stranded probe. The reaction standard free energy of hybridization between the probe and target nucleic acid as determined by Expression 1 (?G°rxn=?G°t-TC??G°nh-PC+(?G°v-TC??G°h-PC)) is from about ?4 kcal/mol to about +4 kcal/mol. Alternatively, the reaction standard free energy of hybridization between the probe and target nucleic acid is determined by Expression 1 to be within 5 kcal/mol of the standard free energy as determined by Expression 2 (?R? ln(([P]0?[C]0)/[C]0)]), where the [P]0 term of Expression 2 equals the concentration of the protector strand and the [C]0 term of Expression 2 equals the concentration of the complement strand. In addition, a method for on-the-fly fine tuning of a reaction using the present probe is provided.
    Type: Grant
    Filed: June 6, 2016
    Date of Patent: January 26, 2021
    Assignee: William Marsh Rice University
    Inventors: David Yu Zhang, Juexiao Wang, Ruojia Wu
  • Patent number: 10894980
    Abstract: Methods are provided for nucleic acid amplification including contacting a double stranded nucleic acid with transposases bound to transposon DNA, wherein the transposon DNA includes a transposase binding site and an RNA polymerase promoter sequence, wherein the transposases/transposon DNA complex bind to target locations along the double stranded nucleic acid and cleave the double stranded nucleic acid into a plurality of double stranded fragments, with each double stranded fragment having the transposon DNA bound to each 5? end of the double stranded fragment, extending the double stranded fragments along the transposon DNA to make double stranded extension products having double stranded RNA polymerase promoter sequences at each end, contacting the double stranded extension products with an RNA polymerase to make a plurality of RNA transcripts of each double stranded extension product, reverse transcribing the RNA transcripts into single stranded copy DNA, forming complementary strands to the single strand
    Type: Grant
    Filed: July 15, 2016
    Date of Patent: January 19, 2021
    Assignee: President and Fellows of Harvard College
    Inventors: Chongyi Chen, Dong Xing, Xiaoliang Sunney Xie
  • Patent number: 10886006
    Abstract: The invention can be used to provide a more efficient and less error-prone method of detecting variants in DNA, such as SNPs and indels. The invention also provides a method for performing inexpensive multiplex assays. The invention also provides methods for detection of DNA sequences altered after cleavage by a targetable endonuclease, such as the CRISPR Cas9 protein from the bacterium Streptococcus pyogenes.
    Type: Grant
    Filed: April 13, 2017
    Date of Patent: January 5, 2021
    Assignee: INTEGRATED DNA TECHNOLOGIES, INC.
    Inventors: Joseph Dobosy, Caifu Chen, Mark Aaron Behlke, Garrett Richard Rettig
  • Patent number: 10876156
    Abstract: The present invention generally relates to microfluidics and, in particular, to systems and methods for determining cells using amplification. In one set of embodiments, cells are encapsulated within droplets and nucleic acids from the cells amplified within the droplets. The droplets may then be pooled together and the amplified nucleic acids can be determined using PCR or other suitable techniques. In some embodiments, techniques such as these can be used to detect relatively rare cells that may be present, e.g., if the droplets are amplified using conditions able to selectively amplify nucleic acids arising from the relatively rare cells.
    Type: Grant
    Filed: March 11, 2016
    Date of Patent: December 29, 2020
    Assignee: President and Fellows of Harvard College
    Inventors: David A. Weitz, Huidan Zhang
  • Patent number: 10858661
    Abstract: The present invention relates to a method for producing various metabolites from a gas-phase alkane compound using a Methylomonas sp. DH-1 strain, deposited under Accession Number KCTC18400P, or a transformant thereof. The method provided in the present invention enables more effective production of various metabolites from a gaseous alkane compound compared to the conventional method using methanotrophic bacteria, and thus, the method of the present invention can be widely used for production of a target material using a bioreactor.
    Type: Grant
    Filed: January 10, 2018
    Date of Patent: December 8, 2020
    Assignee: University-Industry Cooperation Group of Kyung Hee University
    Inventors: Eun Yeol Lee, In Yeub Hwang, So Hyeon Oh, Ok Kyung Lee, Young Chan Jeon, Thi Ngoc Diep Nguyen, Duc Anh Nguyen, Hye Jin Kim, Thi Thu Nguyen
  • Patent number: 10837065
    Abstract: Embodiments of present disclosure are directed to methods for amplifying nucleic acid, comprising two steps: a first step of preparing a reaction mixture comprising the target nucleic acid and a second step of processing the reaction mixture in a thermocycler. During a first phase of the processing step, the thermocycler may be configured to heat the reaction mixture to a first temperature and cool the reaction mixture to a second temperature repeatedly for a first plurality of cycles. During the first phase, fluorescence probes do not anneal to template strands and do not emit fluorescence signals. During a second phase of the processing step, the thermocycler may heat the reaction mixture to a third temperature and cool the reaction mixture to a fourth temperature repeatedly for a second plurality of cycles.
    Type: Grant
    Filed: August 12, 2016
    Date of Patent: November 17, 2020
    Assignee: TETRACORE, INC.
    Inventors: William M. Nelson, Kyle Armantrout, Tracy Calvin Fecteau
  • Patent number: 10835607
    Abstract: Described here is a method for reducing or preventing Pseudomonas aeruginosa biofilm formation in a human subject in need thereof, comprising administering to the human subject a first composition comprising (a) an antigen-binding polypeptide that binds Pf-family bacteriophage, or (b) a vaccine against Pf-family bacteriophage. Also described is an antigen-binding polypeptide that binds specifically to a CoaB protein of Pf-family bacteriophage or fragment thereof.
    Type: Grant
    Filed: January 30, 2019
    Date of Patent: November 17, 2020
    Assignee: INIMMUNE CORPORATION
    Inventors: Paul L. Bollyky, William Parks, Patrick Secor
  • Patent number: 10837967
    Abstract: Compounds of general formula I wherein Fc and Fc? may be the same or different and are substituted ferrocenyl moieties having at least one ring substituent selected from sulfur-containing groups, phosphorus-containing groups, iodo, chloro, silyl, fluoroalkyl groups containing two or more fluorine atoms, heteroaryl, substituted phenyl, and cyano, wherein if present as sole substituent the cyano group is located on the proximal cyclopentadienyl ring; X is a spacer, Y is a spacer, Z is a spacer; and R is a linker group. Compound I may be used to make labelled substrates, functionalised compounds for making labelled substrates and may be used as labels in an electrochemical assay.
    Type: Grant
    Filed: October 25, 2019
    Date of Patent: November 17, 2020
    Assignee: Binx Health Limited
    Inventors: Barrie Marsh, Christopher Frost, David Pearce
  • Patent number: 10830728
    Abstract: Compounds of general formula I are used as labels in an electrochemical assay: (I) in which: Fc and Fc? are substituted or unsubstituted ferrocenyl moieties, X is a C1 to C6 alkylene chain which is optionally interrupted by —O— or —NH—; Y is a C1 to C6 alkylene chain which is optionally interrupted by —O— or —NH—; Z is a C1 to C12 alkylene chain which may optionally be substituted and/or may optionally be interrupted by —O—, —S—, cycloalkyl, —CO—, —CON R1—, —NR1CO— or —NR1— in which R1 represents hydrogen or C1 to C4 alkyl; and R is a linker group. Compounds I are used to make labelled substrates, as well as functionalised compounds for making the labelled substrates.
    Type: Grant
    Filed: October 29, 2019
    Date of Patent: November 10, 2020
    Assignee: Binx Health Limited
    Inventors: Barrie Marsh, Jonathan Sharp, Stephen Flower, Christopher Frost
  • Patent number: 10760071
    Abstract: A system for continuous mutagenesis to facilitate directed evolution, the system including DNA polymerases carrying the novel K54E point mutation, and other point mutations including I709N, A759R, D424A (herein called K54E_LF Pol I) and this methods of use to produce and detect lines where mutagenesis is continuous and does not exhibit the usual decline in mutagenesis with sequential cloning.
    Type: Grant
    Filed: July 25, 2016
    Date of Patent: September 1, 2020
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventor: Manel Camps
  • Patent number: 10761090
    Abstract: The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.
    Type: Grant
    Filed: January 25, 2017
    Date of Patent: September 1, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Michael L. Samuels, Darren Roy Link
  • Patent number: 10752939
    Abstract: Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of cleavable probes that comprise a ribonucleotide position that is susceptible to endoribonuclease (e.g., RNase H) cleavage in the presence of target nucleic acid molecules. Probes of the embodiments may also comprise non-natural nucleotide linked to a reporter and/or quenching moiety.
    Type: Grant
    Filed: July 21, 2017
    Date of Patent: August 25, 2020
    Assignee: LUMINEX CORPORATION
    Inventors: Scott C. Johnson, Nicolas Arab, Doug Whitman
  • Patent number: 10731217
    Abstract: Methods and kits are provided for diagnosing, monitoring, or predicting preeclaimpsia in a pregnant woman, trisomy 18 and trisomy 21 in a fetus, as well as for detecting pregnancy in a woman, by quantitatively measuring in the maternal blood the amount of one or more RNA species derived from a set of genetic loci and comparing the amount of the RNA species with a standard control.
    Type: Grant
    Filed: May 21, 2018
    Date of Patent: August 4, 2020
    Assignee: The Chinese University of Hong Kong
    Inventors: Yuk-Ming Dennis Lo, Rossa Wai Kwun Chiu, Stephen Siu Chung Chim, Nancy Bo Yin Tsui
  • Patent number: 10732183
    Abstract: This invention provides methods for detecting the presence of a plurality of predetermined compounds in a sample using a plurality of tag moieties and at least one nanopore. This invention also provides methods for determining the quantity of each of a plurality of predetermined compounds in a sample using a plurality of tag moieties and at least one nanopore. This invention further provides methods for detecting interaction of at least two predetermined compounds using at least one tag moiety and at least one nanopore.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: August 4, 2020
    Assignee: The Trustees of Columbia University in the City of New York
    Inventors: Jingyue Ju, Shiv Kumar, Chuanjuan Tao, Sergey Kalachikov, James J. Russo
  • Patent number: 10719579
    Abstract: A method for validating quantification of biomarkers, the biomarkers being quantified using a quantification technique of a selected type, and the method including determining a plurality of biomarker values, each biomarker value being indicative of a value measured or derived from a measured value, for at least one corresponding biomarker of the biological subject and being at least partially indicative of a concentration of the biomarker in a sample taken from the subject, determining at least one control value by determining a combination of biomarker values, comparing each control value to a respective control reference and determining if the biomarker values are valid using results of the comparison.
    Type: Grant
    Filed: May 20, 2016
    Date of Patent: July 21, 2020
    Assignee: IMMUNEXPRESS PTY LTD
    Inventor: Leo Charles McHugh
  • Patent number: 10702865
    Abstract: Provided is a measurement container including a container body where a first region, a second region, and an intermediate region are continuously formed as an accommodation space accommodating a sample and an inlet is formed and a rotor which is arranged in the second region and rotated by an effect of magnetic force. The container body includes a wall portion which defines the accommodation space and at least a portion of which has a light transmitting property and a partitioning portion which partitions the intermediate region into one side and the other side in a second direction perpendicular to the first direction. The partitioning portion partitions the intermediate region into the one side and the other side so that a communication region which allows the one side and the other side to communicate with each other in the intermediate region remains.
    Type: Grant
    Filed: August 9, 2017
    Date of Patent: July 7, 2020
    Assignee: HAMAMATSU PHOTONICS K.K.
    Inventors: Kimiko Kazumura, Hiroshi Tsuchiya
  • Patent number: 10697973
    Abstract: The present invention provides a method for identifying a synthetic classifier including contacting at least a first and second samples derived from different groups of a cohort with a first plurality of peptides. The first plurality of peptides includes a first subset of peptides defining at least one naturally occurring amino acid sequence, and a second subset of peptides defining a plurality of variants of the first subset of peptides. The plurality of variants includes, for each one of the first subset of peptides, a variant peptide having at least one of a substitution, a deletion, an insertion, an extension, and a modification. The method further includes selecting at least one of the plurality of variants from the second subset of peptides, and defining a synthetic classifier including the at least one of the plurality of variants that distinguishes between samples derived from the first and second cohorts.
    Type: Grant
    Filed: September 29, 2017
    Date of Patent: June 30, 2020
    Assignee: Roche Sequencing Solutions, Inc.
    Inventors: Hanying Li, Ken Lo, Jigar Patel
  • Patent number: 10696988
    Abstract: A combination of an electrochemical device for delivering reducing equivalents to a cell, and engineered metabolic pathways within the cell capable of utilizing the electrochemically provided reducing equivalents is disclosed. Such a combination allows the production of commodity chemicals by fermentation to proceed with increased carbon efficiency.
    Type: Grant
    Filed: February 23, 2016
    Date of Patent: June 30, 2020
    Assignee: Biocheminsights, Inc.
    Inventors: David R. Dodds, William B. Armiger, Mattheos Koffas