Fungi (e.g., Yeast, Mold, Etc.) Patents (Class 435/7.31)
  • Patent number: 10512223
    Abstract: A consumer product for supplementing CO2 is provided with delayed activation control and multiple chambers formed by folding and clamping or with multiple clamps. A bag, having a top and a bottom seal and a filter, contains organisms and food substrate, each prepared under sterile laboratory conditions using aseptic techniques. The bag may be selectively clamped at various locations on its exterior. The mycelial mass having mycelia and food, or mycelia separate from the food may be sealed or partially sealed away from the air exchange portal by clamps or folds. With selective chamber isolation, the organisms are manipulated to delay optimized carbon dioxide generation. Utilizing the present invention will extend the product shelf-life, delaying the expiration of the product for storage and shipping. The fungi are utilized on-demand by removal of the clamp and carbon dioxide flows from the product. Consumers will place the product near indoor plants.
    Type: Grant
    Filed: January 15, 2018
    Date of Patent: December 24, 2019
    Inventors: Glen Babcock, Wendy Babcock Garrett
  • Patent number: 10094793
    Abstract: Certain embodiments of the invention are directed to a photothermal immunoassay employing a thermometer or colorimetric detection method for sensitive quantitative readout based on the photothermal effect provided by a detection reagent.
    Type: Grant
    Filed: September 19, 2016
    Date of Patent: October 9, 2018
    Assignee: THE BOARD OF REGENTS OF THE UNIVERSITY OF TEXAS SYSTEM
    Inventors: XiuJun Li, Guanglei Fu
  • Patent number: 9745589
    Abstract: This invention is based, at least in part, on the discovery that Shn2 and Shn3 play an important role in skeletal remodeling and skeletal patterning. Accordingly, the present invention provides methods for identifying medulators of Shn2 activity and methods for modulating bone formation and mineralization and Shn2-associated disorders using agents that modulate Shn2 expression and/or activity, in addition to methods for modulating Shn2 and Shn3.
    Type: Grant
    Filed: January 13, 2011
    Date of Patent: August 29, 2017
    Assignee: Cornell University
    Inventors: Laurie H. Glimcher, Dallas C. Jones, Marc Wein
  • Patent number: 9551015
    Abstract: The present invention relates to a process for the production of one or more fermentation product from a sugar composition, comprising the following steps: a) fermentation of the sugar composition in the presence of a yeast belonging to the genera Saccharomyces, Kluyveromyces, Candida, Pichia, Schizosaccharomyces, Hansenula, Kloeckera, Schwanniomyces or Yarrowia,: and b) recovery of the fermentation product, wherein the yeast comprises the genes araA, araB and araD and the sugar composition comprises glucose, galactose and arabinose.
    Type: Grant
    Filed: July 6, 2010
    Date of Patent: January 24, 2017
    Assignee: DSM IP ASSETS B.V.
    Inventors: Paul Klaassen, Gijsberdina Pieternella Van Suylekom, Bianca Elisabeth Maria Gielesen, Nicolette Jasmijn Broers, Beate Wiedemann, Wilhelmus Theodorus Antonius Maria De Laat
  • Patent number: 9516879
    Abstract: Described is an antigen-binding protein, preferably comprising an amino acid sequence that comprises four framework regions and three complementarity-determining regions, wherein the antigen-binding protein is capable of binding a chitinous polysaccharide, and uses thereof.
    Type: Grant
    Filed: August 26, 2011
    Date of Patent: December 13, 2016
    Assignee: Agrosavfe N.V.
    Inventors: Peter Verheesen, Chris De Jonghe, Erik Jongedijk
  • Patent number: 9435806
    Abstract: An immunochromatographic test strip that achieves a shorter reaction completion time and excellent sensitivity is provided. The immunochromatographic test strip comprises: (1) a conjugate pad comprising a sample-supplying section supplying a sample possibly containing an analyte and a line-shaped conjugate section containing a conjugate in which an antibody or antigen immunologically reactive with the analyte is immobilized to a label on the downstream side relative to the sample-supplying section; and (2) an insoluble membrane support having at least one detecting section to which an antibody or antigen immunologically reactive with the analyte is immobilized, the lower surface of the sample-supplying section of the conjugate pad being not in contact with the upper surface of the insoluble membrane support, the lower surface of the conjugate section of the conjugate pad being in contact with the upper surface of the insoluble membrane support.
    Type: Grant
    Filed: September 29, 2011
    Date of Patent: September 6, 2016
    Assignee: SEKISUI MEDICAL CO., LTD.
    Inventors: Kumiko Yuki, Yuka Sasaki
  • Patent number: 9052323
    Abstract: An osmolyte composition comprising 4 M glycerol and 4M urea for stabilizing previously transient protein folding intermediates as long-lived stable forms. A method to search for other possible stabilizing osmolyte mixtures using a screening array is also provided. These additional osmolyte mixtures may complement or augment the successful 4M glycerol/4 M urea mixture.
    Type: Grant
    Filed: August 27, 2010
    Date of Patent: June 9, 2015
    Assignee: The University of Kansas
    Inventors: Mark T. Fisher, Hiroo Katayama
  • Publication number: 20150132777
    Abstract: The present invention provides a method of detecting dermatophyte, which does not require a complicated operation such as an enzyme treatment and a heat treatment. The present invention provides a method of detecting dermatophyte, including a step of extracting a dermatophyte component from a sample with a treatment liquid containing a non-ionic surfactant or a zwitterionic surfactant, and a kit for diagnosing dermatophyte infection, containing a treatment liquid comprising the above surfactant, and an antibody specifically recognizing a dermatophyte component, which are housed in separate containers.
    Type: Application
    Filed: January 16, 2015
    Publication date: May 14, 2015
    Inventor: Sakon NORIKI
  • Publication number: 20150132740
    Abstract: A concentration agent for microorganisms is provided that contains both lanthanum and carbonate. Additionally, articles that include the concentration agent and methods of concentrating a microorganism using the concentration agent are provided.
    Type: Application
    Filed: May 23, 2013
    Publication date: May 14, 2015
    Inventors: Manjiri T. Kshirsagar, Evan Koon Lun Yuuji Hajime, Andrew W. Rabins
  • Patent number: 9017956
    Abstract: Provided herein are uses of genes for HOG, Ras and cAMP signal transduction pathways to treat fungal infection. To regulate the HOG pathway of Cryptococcus neoformans, roles of SSK1, TCO2, SSK2, PBS2, HOG1, ENA1 and NHA1 genes were investigated to find that a biosynthesis level of ergosterol is increased when these genes are inhibited. When the genes are inhibited, a large amount of ergosterol is distributed on a fungal cell membrane. Accordingly, since there are many working points of an ergosterol-binding antifungal agent, an efficiency of the ergosterol-binding antifungal agent can be considerably improved. To regulate the Ras and cAMP pathways of Cryptococcus neoformans, roles of RAS1, RAS2, CDC24, GPA1, CAC1, ACA1, PKA1, HSP12 and HSP122 genes were investigated to find that a sensitivity to a polyene- or azole-based drug is increased when these genes are inhibited.
    Type: Grant
    Filed: April 15, 2013
    Date of Patent: April 28, 2015
    Assignee: Nutrex Technology Co., Ltd.
    Inventors: Yong-Sun Bahn, Young-Joon Ko, Shin-Ae Maeng, Kwang Woo Jung, Gyu Bum Kim
  • Publication number: 20150111784
    Abstract: In some cases, the described systems and methods include obtaining a cell sample containing multiple antibody-producing cells. In such cases, the cells can be tagged with a cross-linking reagent having a first portion configured to bind to a marker on the antibody-producing cells and a second portion configured to bind to an antigen of interest. In some instances, the tagged antibody-producing cells are exposed to the antigen of interest such that the antigen becomes bound to the cells. In some such instances, the antibody-producing cells are also allowed to produce an antibody, such that a portion of the antibody-producing cells produce an antigen-specific antibody that binds to the antigen of interest. To identify cells that produce the antigen-specific antibody, the tagged cells can be exposed to a labeled secondary antibody that is configured to bind to the antigen-specific antibody. Other implementations are also described.
    Type: Application
    Filed: December 26, 2014
    Publication date: April 23, 2015
    Inventor: Kevin T. Chapman
  • Publication number: 20150098905
    Abstract: The present invention relates to targeting agents and methods: of using the targeting agents to detect a fungal cell in a subject.
    Type: Application
    Filed: May 8, 2013
    Publication date: April 9, 2015
    Applicant: Rutgers, The State University of New Jersey
    Inventors: David S. Perlin, Guillermo Garcia-Effron, Arkady Mustaev
  • Publication number: 20150098951
    Abstract: The invention relates to modulation of fungal morphology between yeast-to-hyphal growth transition by controlling muramyl-L-alanine concentration and uses thereof.
    Type: Application
    Filed: October 17, 2014
    Publication date: April 9, 2015
    Applicant: Agency for Science, Technology and Research
    Inventors: Yue Wang, Xiao-Li Xu, Raymond Teck Ho Lee
  • Publication number: 20150072358
    Abstract: A hand-held measuring instrument is used for detecting hidden mold damage in interior spaces. The measuring instrument includes at least one collection unit configured to intake at least of one interior space sample, at least one buffer unit, at least one immunological test unit, an evaluation unit and a control panel. The at least one buffer unit has a first compartment and a second compartment.
    Type: Application
    Filed: September 5, 2014
    Publication date: March 12, 2015
    Inventor: Katrin Luckert
  • Publication number: 20150072880
    Abstract: A device for detecting at least one analyte generally comprises (i) a support having a chamber for receiving a biological fluid therein, wherein said chamber is an elongate chamber having a length axis; (ii) a carrier or agitator in said elongate chamber, said carrier or agitator having opposite end portions and a side portion, the carrier or agitator dimensioned to travel in said chamber along said length axis and/or permit said liquid sample to flow in the chamber therearound, either (or both) thereby agitating the liquid sample; and (iii) at least one anti-analyte antibody coupled to either the carrier and/or the chamber side wall. Methods of using the device are also described.
    Type: Application
    Filed: May 9, 2013
    Publication date: March 12, 2015
    Inventors: David A. Calderwood, Rodolfo R. Rodriguez
  • Publication number: 20150064112
    Abstract: The invention relates to compounds that specifically bind a T1R1/T1R3 or T1R2/T1R3 receptor or fragments or sub-units thereof. The present invention also relates to the use of hetero-oligomeric and chimeric taste receptors comprising T1R1/T1R3 and T1R2/T1R3 in assays to identify compounds that respectively respond to umami taste stimuli and sweet taste stimuli. Further, the invention relates to the constitutive of cell lines that stably or transiently co-express a combination of T1R1 and T1R3; or T1R2 and T1R3; under constitutive or inducible conditions. The use of these cells lines in cell-based assays to identify umami and sweet taste modulatory compounds is also provided, particularly high throughput screening assays that detect receptor activity by use of fluorometric imaging.
    Type: Application
    Filed: September 4, 2014
    Publication date: March 5, 2015
    Inventors: XIAODONG LI, LENA STASZEWSKI, HONG XU
  • Patent number: 8962264
    Abstract: The present invention provides a method of detecting dermatophyte, which does not require a complicated operation such as an enzyme treatment and a heat treatment. The present invention provides a method of detecting dermatophyte, including a step of extracting a dermatophyte component from a sample with a treatment liquid containing a non-ionic surfactant or a zwitterionic surfactant, and a kit for diagnosing dermatophyte infection, containing a treatment liquid comprising the above surfactant, and an antibody specifically recognizing a dermatophyte component, which are housed in separate containers.
    Type: Grant
    Filed: March 19, 2007
    Date of Patent: February 24, 2015
    Assignees: National University Corporation University of Fukui, JNC Corporation
    Inventor: Sakon Noriki
  • Publication number: 20150030661
    Abstract: The invention provides, inter alia, multilayer films of alternating layers of a glycosylated polymer (e.g. a mucin) and a lectin, as well as methods of making and using these films. The films can be adapted for, inter alia, delivery of a biologically active agent providing a non-toxic substrate to support cell growth, replication, and/or maintenance as well as detecting a microorganism and/or reducing microorganism adherence to a surface.
    Type: Application
    Filed: February 6, 2013
    Publication date: January 29, 2015
    Inventors: Katharina Ribbeck, Thomas Crouzier
  • Patent number: 8932825
    Abstract: The present invention relates to host cells having modified lipid-linked oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar transporters and mannosidases to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified lipid-linked oligosaccharides are created or selected. N-glycans made in the engineered host cells have a GlcNAcMan3GlcNAc2 core structure which may then be modified further by heterologous expression of one or more enzymes, e.g., glycosyl-transferases, sugar transporters and mannosidases, to yield human-like glycoproteins. For the production of therapeutic proteins, this method may be adapted to engineer cell lines in which any desired glycosylation structure may be obtained.
    Type: Grant
    Filed: December 24, 2002
    Date of Patent: January 13, 2015
    Assignee: GlycoFi Inc.
    Inventors: Stefan Wildt, Robert Gordon Miele, Juergen Hermann Nett, Robert C. Davidson
  • Publication number: 20150010903
    Abstract: The present invention relates to a method for the detection of a substance in an aqueous, physiological or chemical liquid using the evanescence field method, and to a diagnostic device for carrying out said method.
    Type: Application
    Filed: March 12, 2012
    Publication date: January 8, 2015
    Applicant: DAVOS DIAGNOSTICS AG
    Inventors: Manfred Schawaller, Claudio Rhyner, Cezmi Akdis, Max Wiki, Reto Crameri
  • Patent number: 8911959
    Abstract: Coccidioidomycosis (Valley Fever) is currently diagnosed by relying on infected individuals (humans, dogs, etc.) to generate antibodies against the fungus. Since the immune response against Valley Fever can be delayed or absent in many cases, methods that detect a polypeptide or peptide component of the Coccidioides sp. fungus in a bodily fluid such as blood are provided.
    Type: Grant
    Filed: September 10, 2012
    Date of Patent: December 16, 2014
    Assignees: Arizona Board of Regents, a body corporate of the State of Arizona Acting for and on behalf of Arizona State University, Mayo Foundation for Medical Education and Research
    Inventors: Douglas Lake, Kwasi Antwi, Janis E. Blair
  • Publication number: 20140357508
    Abstract: Aspects of the invention provide compositions and methods for displaying engineered polypeptides on a cell surface. According to aspects of the invention, immobilized polypeptides can be screened to identify one or more variants having one or more functional or structural properties of interest. Aspects of the invention provide composition and methods for producing engineered protein or protein variants having a functional or a structural property of interest.
    Type: Application
    Filed: March 26, 2014
    Publication date: December 4, 2014
    Inventors: James A. Rakestraw, Stan I. Letovsky, Shaun Lippow
  • Publication number: 20140357521
    Abstract: The disclosure relates cells or cellular systems that express both a membrane protein and a binding domain directed to the membrane protein. Also, methods are provided that use such cells or cellular systems to produce higher amounts of the membrane proteins. Further, the cells or cellular systems can be used as tools for the structural and functional characterization of membrane proteins, as well as for screening and drug discovery efforts targeting membrane proteins.
    Type: Application
    Filed: January 21, 2013
    Publication date: December 4, 2014
    Inventors: Jan Steyaert, Nico Callewaert, Toon Laeremans, Els Pardon, Kristof Vandewalle, Katrien Claes
  • Publication number: 20140342932
    Abstract: Systems for making, identifying, and selecting recombinant cells that express a ligand for the insulin receptor (IR) or insulin growth factor I (IGF-1) receptor are described. In general, libraries of recombinant cells are constructed that are capable of displaying a plurality of ligand molecules on the cell surface. Recombinant cells that display a ligand in a form accessible for binding to the IR and/or IGF-1 receptor can be detected and the recombinant cells displaying said ligands can be selected and isolated using cell sorting technologies. In particular aspects, the system is useful for constructing and screening libraries of recombinant cells that express and displaying insulin analogue precursors molecules to identify and select recombinant cells in the library that bind the IR and/or IGF-1 receptor with a desired affinity and/or avidity.
    Type: Application
    Filed: September 18, 2012
    Publication date: November 20, 2014
    Inventors: Ming-Tang Chen, Byung-Kwon Choi, Song Lin, Natarajan Sethuraman, Hussam Shaheen, Terrance Stadheim, Dongxing Zha
  • Publication number: 20140323340
    Abstract: The present invention relates to moving microorganisms to a surface, where they are grown in the presence and absence of antimicrobials, and by monitoring the growth of the microorganisms over time in the two conditions, their susceptibility to the antimicrobials can be determined. The microorganisms can be moved to the surface through electrophoresis, centrifugation or filtration. When the movement involves electrophoresis, the presence of oxidizing and reducing reagents lowers the voltage at which electrophoretic force can be generated and allows a broader range of means by which the target can be detected. Monitoring can comprise optical detection, and most conveniently includes the detection of individual microorganisms. The microorganisms can be stained in order to give information about their response to antimicrobials.
    Type: Application
    Filed: July 11, 2014
    Publication date: October 30, 2014
    Inventors: David A. Goldberg, David C. Howson, Steven W. Metzger, Daniel A. Buttry, Steven Scott Saavedra
  • Publication number: 20140323348
    Abstract: Disclosed are methods of promoting and/or enhancing G-Protein Coupled Receptor (GPCR) localization to the cell membrane and/or cell surface; methods of promoting and/or enhancing GPCR functional expression; and methods and assays for screening or identifying ligands (e.g., agonists or antagonists) that bind GPCRs. Also provided are vectors, recombinant cells, and stable cell lines for use in the methods and assays.
    Type: Application
    Filed: November 30, 2012
    Publication date: October 30, 2014
    Inventors: Hiroaki Matsunami, Yue Jiang, Ming-Shan Chien
  • Publication number: 20140315219
    Abstract: The present invention provides a rapid, highly sensitive and specific enzyme-linked immunosorbent assay (ELISA), referred to as N-Assay, a device, and a kit, for detection and identification of microorganisms in a sample, in thirty minutes or less, with little or no interference from non-target microorganisms. The present invention also provides for simultaneous determination of antimicrobial susceptibility of microorganism in the N-Assay.
    Type: Application
    Filed: April 18, 2014
    Publication date: October 23, 2014
    Inventors: Bret T. Barnhizer, Jonathan P. Faro
  • Patent number: 8865411
    Abstract: Compositions and methods are disclosed for identifying agents useful for the treatment of proteinopathies.
    Type: Grant
    Filed: March 26, 2010
    Date of Patent: October 21, 2014
    Assignees: National Institutes of Health (NIH), U.S. Dept. of Health and Human Services (DHHS), NIH Division of Extramural Inventions and Technology Resources (DEITR)
    Inventors: Aaron D. Gitler, Andrew Elden
  • Patent number: 8846326
    Abstract: The invention relates to compounds that specifically bind a T1R1/T1R3 or T1R2/T1R3 receptor or fragments or sub-units thereof. The present invention also relates to the use of hetero-oligomeric and chimeric taste receptors comprising T1R1/T1R3 and T1R2/T1R3 in assays to identify compounds that respectively respond to umami taste stimuli and sweet taste stimuli. Further, the invention relates to the constitutive of cell lines that stably or transiently co-express a combination of T1R1 and T1R3; or T1R2 and T1R3; under constitutive or inducible conditions. The use of these cells lines in cell-based assays to identify umami and sweet taste modulatory compounds is also provided, particularly high throughput screening assays that detect receptor activity by use of fluorometric imaging.
    Type: Grant
    Filed: February 19, 2013
    Date of Patent: September 30, 2014
    Assignee: Senomyx, Inc.
    Inventors: Xiaodong Li, Lena Staszewski, Hong Xu
  • Patent number: 8828737
    Abstract: Methods for using focused light scattering techniques for the optical sensing of biological particles suspended in a liquid medium are disclosed. The optical sensing enables one to characterize particles size and/or distribution in a given sample. This, in turn, allows one to identify the biological particles, determine their relative particle density, detect particle shedding, and identify particle aggregation. The methods are also useful in screening and optimizing drug candidates, evaluating the efficacy and dosage levels of such drugs, and in personalized medicine applications.
    Type: Grant
    Filed: July 14, 2009
    Date of Patent: September 9, 2014
    Assignee: Invitrox, Inc.
    Inventor: Don Gabriel
  • Publication number: 20140248605
    Abstract: The present invention includes a method of observing and evaluating bacterial infections within the lymph nodes of animals presented for harvest comprising: inoculating at one or more sites of an animal a known amount of a pathogen, wherein the one or more inoculation sites comprise lymph node drainage areas, and at one or more time points obtaining one or more lymph node biopsies to determine the extent of the pathogen in the lymph nodes.
    Type: Application
    Filed: March 4, 2014
    Publication date: September 4, 2014
    Applicant: Texas Tech University System
    Inventors: Guy Loneragan, Thomas Edrington
  • Patent number: 8815580
    Abstract: The present application relates to the field of glyco-engineering, more specifically to eukaryotic cells wherein both an endoglucosaminidase and a glycoprotein are present. These cells can be used to deglycosylate or partly deglycosylate the (exogenous) glycoprotein, in particular without the need for adding an extra enzyme. Methods are also provided for the application of these cells in protein production. According to one specific aspect, the eukaryotic cells and methods are glyco-engineered yeast cells in which additionally at least one exogenous enzyme needed for complex glycosylation is present, e.g. allowing easier separation of differentially glycosylated glycoproteins.
    Type: Grant
    Filed: August 10, 2009
    Date of Patent: August 26, 2014
    Assignees: VIB VZW, Universiteit Gent
    Inventors: Nico L. M. Callewaert, Karen De Pourcq, Steven Geysens, Leander Meuris
  • Publication number: 20140234831
    Abstract: Provided herein is a composition for detection of a microbial product in a sample comprising a polydiacetylene (PDA) and a packaging polymer, wherein the sample contacts the PDA and a change of PDA color indicates detection. Also provided herein are methods of making a packaging material comprising a PDA and methods of using the composition for detection of a microbial product in a sample.
    Type: Application
    Filed: September 28, 2012
    Publication date: August 21, 2014
    Inventor: Richard Awdeh
  • Patent number: 8809001
    Abstract: The subject invention provides methods of screening compounds or ligands that interact with human and/or non-human PAQR receptors or fungal osmotin receptors. These methods utilize a colorimetric assay to ascertain whether a compound binds to and activates a PAQR receptor or the osmotin receptor.
    Type: Grant
    Filed: September 23, 2008
    Date of Patent: August 19, 2014
    Assignee: University of Florida Research Foundation, Inc.
    Inventors: Thomas Lyons, Brian R. Kupchak, Jessica L. Smith, Ibon Garitaonandia
  • Patent number: 8802378
    Abstract: The invention relates to certain methods for the determination of an antigen content of a first antigen in a mixture comprising two or more antigens. The invention also relates to a potency test for an antigen in a combination vaccine. The method allows the determination of the antigen content in a mixture additionally comprising antibodies that are capable of binding with the antigen.
    Type: Grant
    Filed: February 5, 2013
    Date of Patent: August 12, 2014
    Assignees: Intervet Inc., Intervet International B.V.
    Inventors: Michelle Allen, Mark Garrett, Urs Peter Bruderer, Martinus Antonius Johannes Thijssen
  • Publication number: 20140186334
    Abstract: The present invention provides, in part, an antibody display system that simultaneously uses a secretion and a display mode. A bait complexed with a monovalent antibody fragment can be expressed on the surface of the host cell wherein the fragment may be assayed for antigen binding while full antibody is simultaneously secreted from the host cell. Methods of using the system for identifying antibodies that bind specifically to an antigen of interest are also provided. Polypeptides, polynucleotides and host cells useful for making the antibody display system are also provided along with methods of use thereof.
    Type: Application
    Filed: November 29, 2011
    Publication date: July 3, 2014
    Inventors: Dongxing Zha, Hussam Hisham Shaheen
  • Publication number: 20140178884
    Abstract: Disclosed herein are methods of diagnosing, monitoring and treating an Aspergillus species-associated condition, such as Aspergillus fumigatus (Af)-associated condition, including aspergillosis, such as invasive aspergillosis (IA). Methods for diagnosing and/or monitoring an Aspergillus species-associated condition, such as IA are provided. Also disclosed are point-of-care immunoassays that can be used to diagnose or monitor the efficacy of an Aspergillus-associated condition treatment. These immunoassays can also be used for rapid diagnosis of infection produced by an Aspergillus species, such as Af.
    Type: Application
    Filed: May 22, 2012
    Publication date: June 26, 2014
    Applicant: BOARD OF REGENTS OF THE NEVADA SYSTEM OF HIGHER EDUCATION, ON BEHALF OF THE UNIV. OF NEVADA, RENO
    Inventors: David Aucoin, Thomas R. Kozel, Sindy Chaves
  • Publication number: 20140178902
    Abstract: The present invention relates to a method for detecting infections caused by or associated with siderophore-secreting microorganisms and kits and components used therein for carrying out the method. In one aspect the invention relates to a method for detecting siderophores and/or detecting infections caused by or associated with siderophore-secreting microorganisms in a biological sample of a subject, the method comprising providing a solid support having either bound siderophore or a conjugate thereof, or bound anti-siderophore antibody; reacting the bound siderophore or a conjugate thereof, with an anti-siderophore antibody, and a biological sample of a subject; or reacting the bound anti-siderophore antibody with a siderophore or conjugate thereof, and a biological sample of a subject; and detecting and/or quantifying the presence of the siderophore in the biological sample.
    Type: Application
    Filed: May 16, 2012
    Publication date: June 26, 2014
    Applicant: NATIONAL UNIVERSITY OF IRELAND, MAYNOOTH
    Inventors: John Martin Doyle, Kieran Walshe, Natasha Gordon, Kevin Kavanagh, Lorna Gallagher
  • Publication number: 20140170639
    Abstract: The invention generally relates to methods for indicating whether an assay for isolating targets is properly isolating and detecting targets. Methods of the invention involve obtaining a sample suspected of a containing target, introducing a detectable marker into the sample, conducting an assay using magnet particles to isolate the detectable marker and the target if it is present in the sample, determining the presence or absence of the target; and confirming that the assay functioned properly by determining presence or absence of the detectable marker.
    Type: Application
    Filed: December 12, 2013
    Publication date: June 19, 2014
    Applicant: NANOMR, INC.
    Inventor: Meghan Norvell
  • Patent number: 8748159
    Abstract: A rapid method for the quantitation of various live cell types is described. This new cell fluorescence method correlates with other methods of enumerating cells such as the standard plate count, the methylene blue method and the slide viability technique. The method is particularly useful in several applications such as: a) quantitating bacteria in milk, yogurt, cheese, meat and other foods, b) quantitating yeast cells in brewing, fermentation and bread making, c) quantitating mammalian cells in research, food and clinical settings. The method is especially useful when both total and viable cell counts are required such as in the brewing industry. The method can also be employed to determine the metabolic activity of cells in a sample. The apparatus, device, and/or system used for cell quantitation is also disclosed.
    Type: Grant
    Filed: October 28, 2011
    Date of Patent: June 10, 2014
    Assignee: GenPrime, Inc.
    Inventors: James E. Fleming, Jason Buck Somes, Darby McLean, Jerad R. Holcomb
  • Patent number: 8735072
    Abstract: Methods of screening candidate agents to identify lead compounds for the development of therapeutic agents for the treatment of a neurodegenerative disease, such as Huntington's Disease and Parkinson's Disease and methods for identifying a mutation in, or changes in expression of, a gene associated with neurodegenerative disease, such as Huntington's Disease and Parkinson's Disease, are provided.
    Type: Grant
    Filed: March 13, 2012
    Date of Patent: May 27, 2014
    Assignees: Whitehead Institute for Biomedical Research, University of Washington
    Inventors: Paul J. Muchowski, Susan L. Lindquist, Tiago Outeiro
  • Patent number: 8648183
    Abstract: It is intended to provide base sequences participating in the regulation of the expression of a promoter. It is also intended to modify a promoter based on the base sequence data to give a modified promoter having a high expression activity. Namely, a modified promoter constructed by inserting a first DNA fragment containing CCAATNNNNNN (SEQ ID NO:1) (a first base sequence) and a second DNA fragment containing CGGNNNNNNNNNGG (SEQ ID NO:2) (a second base sequence) into a promoter functioning in a filamentous fungus, wherein N in the base sequence denotes A (adenine), G (guanine), C(cytosine), or T (thymine).
    Type: Grant
    Filed: February 28, 2003
    Date of Patent: February 11, 2014
    Assignee: Amano Enzyme Inc.
    Inventors: Kanako Suzuki, Norihiro Tsukagoshi
  • Publication number: 20140031292
    Abstract: The present invention provides a genetic method of tethering polypeptides to the yeast cell wall in a form accessible for binding to macromolecules. Combining this method with fluorescence-activated cell sorting provides a means of selecting proteins with increased or decreased affinity for another molecule, altered specificity, or conditional binding. As one embodiment, attaching an scFv antibody fragment to the Aga2p agglutinin effectively mimics the cell surface display of antibodies by B cells in the immune system for affinity maturation in vivo. As another embodiment, T cell receptor mutants can be isolated by this method that are efficiently displayed on the yeast cell surface, providing a means of altering T cell receptor binding affinity and specificity by library screening.
    Type: Application
    Filed: February 12, 2013
    Publication date: January 30, 2014
    Inventors: K. Dane WITTRUP, David M. KRANZ, Michele KIEKE, Eric T. BODER
  • Publication number: 20140011213
    Abstract: The present invention relates to nucleic acid molecules comprising a nucleic acid sequence encoding a membrane-bound biotin mimetic peptide (BMP) or biotin acceptor peptide (BAP). The invention also relates to a method for selection of high producer cells secreting a protein of interest.
    Type: Application
    Filed: December 19, 2011
    Publication date: January 9, 2014
    Applicant: MERCK SERONO S.A.
    Inventors: Daniel Helman, Mira Toister-Achituv, Meirav Bar-Shimon, Moshe Smolarsky
  • Publication number: 20140004538
    Abstract: The present invention concerns an in vitro method for diagnosing invasive candidiasis (IC) in a subject which comprises detecting the presence of a Candida glycan and detecting the presence of antibody directed against a protein selected from the group consisting of fructose bisphosphate aldolase (Fba1), enolase 1 (Eno1), heat shock protein 90 (Hsp90), hyphal wall protein (Hwp1), and mannoprotein 65 (Mp65) in a blood, plasma or serum sample of the subject. The invention also relates to a method of determining a suitable treatment regimen for a patient and to a kit for implanting the methods described herein.
    Type: Application
    Filed: March 18, 2013
    Publication date: January 2, 2014
    Inventors: Sebastien DAMIENS, Chantal FRADIN, Daniel POULAIN, Boualem SENDID, Marc Charles Victor TABOURET
  • Patent number: 8617832
    Abstract: The present invention features a method for identifying an agent that inhibits Candida albicans-mediated differentiation of keratinocytes. Agents identified by the screening assay of the invention find application in the prevention and treatment of candidiasis.
    Type: Grant
    Filed: August 13, 2010
    Date of Patent: December 31, 2013
    Assignee: Trustees of Dartmouth College
    Inventor: Paula Sundstrom
  • Publication number: 20130337437
    Abstract: A process for detecting cells from a sample, comprising the following steps: (a) applying the sample or a fraction of the sample in liquid phase in a direction of flow to a porous, generally two-dimensional support with pores having on the surface thereof at least one binding molecule specific for the cells to be detected or for fragments of such cells; (b) allowing the cells to be detected or of fragments of such cells to enter from the sample or sample fraction into the pores of said porous, generally two-dimensional support, said pores having such a mean pore size that the cells or fragments to be detected are able to enter into the pores; (c) retaining the cells or fragments to be detected by at least one binding molecule specific for the cells or fragments to be detected; (d) performing an optical read-out method on said substantially two-dimensional support by an imaging method, optionally after the cells or cell fragments to be detected have been labeled with a labeling agent; wherein (e) the optica
    Type: Application
    Filed: February 10, 2012
    Publication date: December 19, 2013
    Applicant: fzmb GmbH Forschungszentrum Fuer Medizintechnik Und Biotechnologie
    Inventors: Stephan Henze, Peter Miethe
  • Publication number: 20130336892
    Abstract: The invention relates to compounds that specifically bind a T1R1/T1R3 or T1R2/T1R3 receptor or fragments or sub-units thereof. The present invention also relates to the use of hetero-oligomeric and chimeric taste receptors comprising T1R1/T1R3 and T1R2/T1R3 in assays to identify compounds that respectively respond to umami taste stimuli and sweet taste stimuli. Further, the invention relates to the constitutive of cell lines that stably or transiently co-express a combination of T1R1 and T1R3; or T1R2 and T1R3; under constitutive or inducible conditions. The use of these cells lines in cell-based assays to identify umami and sweet taste modulatory compounds is also provided, particularly high throughput screening assays that detect receptor activity by use of fluorometric imaging.
    Type: Application
    Filed: February 19, 2013
    Publication date: December 19, 2013
    Applicant: SENOMYX, INC.
    Inventors: Xiaodong Li, Lena Staszewski, Hong Xu
  • Publication number: 20130316333
    Abstract: The present invention relates to a method for detecting at least one microorganism in a sample, including: cultivating the sample in a liquid medium in the presence of at least one specific ligand of the microorganism, and at least one scavenger having a lower affinity to the microorganism than the ligand, the binding of a compound to the ligand producing a first measurable signal and the binding of a compound to the scavenger producing a second measurable signal; determining the values of the first and second signals for at least one cultivation period; wherein it is deduced that the sample includes the microorganism when the values of the first signal and second signal are different for the same cultivation period.
    Type: Application
    Filed: November 30, 2011
    Publication date: November 28, 2013
    Applicant: Commissariat A L'Energie Atomique Et Aux Energies Alternatives
    Inventors: Yoann Roupioz, Roberto Calemczuk, Thierry Vernet, Thierry Livache, Sihem Bouguelia, Claire Durmort
  • Publication number: 20130309693
    Abstract: Polypeptides which can be activated to cause the formation of pores in a lipid membrane are disclosed. Also disclosed are polypeptide compositions for the detection of target microorganisms and methods of using said compositions.
    Type: Application
    Filed: July 1, 2013
    Publication date: November 21, 2013
    Applicant: 3M INNOVATIVE PROPERTIES COMPANY
    Inventors: STEPHEN B. ROSCOE, STEPHANIE J. MOELLER, YI HE, RICHARD B. ROSS