Fungi (e.g., Yeast, Mold, Etc.) Patents (Class 435/7.31)
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Publication number: 20130336892Abstract: The invention relates to compounds that specifically bind a T1R1/T1R3 or T1R2/T1R3 receptor or fragments or sub-units thereof. The present invention also relates to the use of hetero-oligomeric and chimeric taste receptors comprising T1R1/T1R3 and T1R2/T1R3 in assays to identify compounds that respectively respond to umami taste stimuli and sweet taste stimuli. Further, the invention relates to the constitutive of cell lines that stably or transiently co-express a combination of T1R1 and T1R3; or T1R2 and T1R3; under constitutive or inducible conditions. The use of these cells lines in cell-based assays to identify umami and sweet taste modulatory compounds is also provided, particularly high throughput screening assays that detect receptor activity by use of fluorometric imaging.Type: ApplicationFiled: February 19, 2013Publication date: December 19, 2013Applicant: SENOMYX, INC.Inventors: Xiaodong Li, Lena Staszewski, Hong Xu
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Publication number: 20130337437Abstract: A process for detecting cells from a sample, comprising the following steps: (a) applying the sample or a fraction of the sample in liquid phase in a direction of flow to a porous, generally two-dimensional support with pores having on the surface thereof at least one binding molecule specific for the cells to be detected or for fragments of such cells; (b) allowing the cells to be detected or of fragments of such cells to enter from the sample or sample fraction into the pores of said porous, generally two-dimensional support, said pores having such a mean pore size that the cells or fragments to be detected are able to enter into the pores; (c) retaining the cells or fragments to be detected by at least one binding molecule specific for the cells or fragments to be detected; (d) performing an optical read-out method on said substantially two-dimensional support by an imaging method, optionally after the cells or cell fragments to be detected have been labeled with a labeling agent; wherein (e) the opticaType: ApplicationFiled: February 10, 2012Publication date: December 19, 2013Applicant: fzmb GmbH Forschungszentrum Fuer Medizintechnik Und BiotechnologieInventors: Stephan Henze, Peter Miethe
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Publication number: 20130316333Abstract: The present invention relates to a method for detecting at least one microorganism in a sample, including: cultivating the sample in a liquid medium in the presence of at least one specific ligand of the microorganism, and at least one scavenger having a lower affinity to the microorganism than the ligand, the binding of a compound to the ligand producing a first measurable signal and the binding of a compound to the scavenger producing a second measurable signal; determining the values of the first and second signals for at least one cultivation period; wherein it is deduced that the sample includes the microorganism when the values of the first signal and second signal are different for the same cultivation period.Type: ApplicationFiled: November 30, 2011Publication date: November 28, 2013Applicant: Commissariat A L'Energie Atomique Et Aux Energies AlternativesInventors: Yoann Roupioz, Roberto Calemczuk, Thierry Vernet, Thierry Livache, Sihem Bouguelia, Claire Durmort
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Publication number: 20130309693Abstract: Polypeptides which can be activated to cause the formation of pores in a lipid membrane are disclosed. Also disclosed are polypeptide compositions for the detection of target microorganisms and methods of using said compositions.Type: ApplicationFiled: July 1, 2013Publication date: November 21, 2013Applicant: 3M INNOVATIVE PROPERTIES COMPANYInventors: STEPHEN B. ROSCOE, STEPHANIE J. MOELLER, YI HE, RICHARD B. ROSS
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Patent number: 8574853Abstract: The present invention relates to monoclonal antibodies that bind or neutralize anthrax lethal factor (LF), edema factor (EF), and/or protective antigen (PA). The invention provides such antibodies, fragments of such antibodies retaining anthrax toxin-binding ability, fully human or humanized antibodies retaining anthrax toxin-binding ability, and pharmaceutical compositions including such antibodies. The invention further provides for isolated nucleic acids encoding the antibodies of the invention and host cells transformed therewith. Additionally, the invention provides for prophylactic, therapeutic, and diagnostic methods employing the antibodies and nucleic acids of the invention.Type: GrantFiled: December 2, 2011Date of Patent: November 5, 2013Assignee: The United States of America, as represented by the Secretary, Department of Health and Human ServicesInventors: Zhaochun Chen, Robert H. Purcell, Suzanne U. Emerson, Stephen H. Leppla, Mahtab Moayeri
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Patent number: 8546149Abstract: The invention relates to certain methods for the determination of an antigen content of a first antigen in a mixture comprising two or more antigens. The invention also relates to a potency test for an antigen in a combination vaccine. The method allows the determination of the antigen content in a mixture additionally comprising antibodies that are capable of binding with the antigen.Type: GrantFiled: September 3, 2010Date of Patent: October 1, 2013Assignees: Intervet Inc., Intervet International B.V.Inventors: Michelle Allen, Mark Garrett, Urs Peter Bruderer, Martinus Antonius Johannes Thijssen
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Patent number: 8541227Abstract: The inventions relates to compositions and method for determining the absolute counts of cells per unit volume of a sample. Such a method comprises: (a) providing a container containing (i) a predetermined quantity of microparticles; and (ii) a cell-binding agent; in which the microparticles are disposed in or on a matrix which adheres to at least one wall of the container such that substantially all the microparticles are thereby attached to the container; (b) adding a known volume of sample to the container; (c) determining the ratio of microparticles to cells by counting microparticles and cells in a volume of the sample; and (d) determining the absolute count of cells by multiplying the number of cells per microparticle by the concentration of microparticles in the sample.Type: GrantFiled: February 6, 2006Date of Patent: September 24, 2013Assignee: Dako Denmark A/SInventors: Nanna K Christensen, Jesper Laursen, Lars Winther
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Patent number: 8541242Abstract: The present invention provides assays and devices for detection of substances in liquid samples. The assays and devices utilize passive diffusion between a porous material and a porous membrane containing a specific binding pair member to enable detection of the substance of interest.Type: GrantFiled: June 29, 2011Date of Patent: September 24, 2013Assignee: Techlab, Inc.Inventors: James H. Boone, David M. Lyerly, Tracy D. Wilkins
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Patent number: 8535665Abstract: The present invention discloses a process for simple and rapid detection and identification of molecular mimicry or mimic antigens or molecules existing in/on humans, animals and plants. The molecular mimicry can be related to infections, autoimmune diseases, cancers, obesity and other disorders. Therefore, novel methods for the diagnosis, prevention, and treatment of infections, autoimmune diseases, cancers, obesity and other disorders obtainable based on these mimic antigens or molecules can be developed. Furthermore, the present invention also reveals a new functional mechanism of vaccine and passive immunity and novel vaccines obtainable based on the new mechanism.Type: GrantFiled: August 17, 2007Date of Patent: September 17, 2013Inventor: Huiru Wang
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Publication number: 20130216997Abstract: Disclosed are methods and systems for the isolation and detection of microbes from a sample. The use of binding agents for isolation of a microbe of interest from a sample are described. In certain embodiments, the methods use ribosome-based and/or bacteriophage-based amplification of the signal in detection of bacteria and other microorganisms. For example, embodiments of the present invention can achieve total amplification of at least 10,000 from a single infected cell.Type: ApplicationFiled: February 21, 2013Publication date: August 22, 2013Applicant: LABORATORY CORPORATION OF AMERICA HOLDINGSInventor: Laboratory Corporation of America Holdings
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Patent number: 8497090Abstract: The present invention relates to fungal strains capable of producing insecticide and a process for production of insecticide. It also relates to a method of cultivation of fungal strains and a fermentation medium for culturing the fungal strains.Type: GrantFiled: October 22, 2008Date of Patent: July 30, 2013Assignee: Indian Institute of ScienceInventors: Ram Rajasekharan, Chikkarasanahalli Shivegowda Vivekbabu
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Patent number: 8492098Abstract: Methods are described for detecting reaction components with affect a reaction. Biomolecules such as enzymes can be addressed at the single molecule level in order to discover function, detect binding partners or inhibitors, and/or measure rate constants.Type: GrantFiled: February 16, 2007Date of Patent: July 23, 2013Assignee: The Trustees of Tufts CollegeInventors: David R. Walt, David M. Rissin, Hans-Heiner Gorris
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Patent number: 8460879Abstract: Arrays of single molecules and methods of producing an array of single molecules are described. Arrays with defined volumes between 10 attoliters and 50 picoliters enable single molecule detection and quantitation.Type: GrantFiled: February 16, 2007Date of Patent: June 11, 2013Assignee: The Trustees of Tufts CollegeInventors: David R. Walt, David M. Rissin
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Patent number: 8460878Abstract: Arrays of single cells and methods of producing an array of single cells are described. Arrays with defined volumes between 10 attoliters and 50 picoliters enable single cell capture, detection and quantitation.Type: GrantFiled: February 16, 2007Date of Patent: June 11, 2013Assignee: The Trustees of Tufts CollegeInventors: David R. Walt, David M. Rissin
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Publication number: 20130108642Abstract: The invention provided Mucorales CotH polypeptides and encoding nucleic acid molecules. The Mucorales CotH polypeptides and encoding nucleic acids can be advantageously used to diagnose, treat or prevent fungal conditions, in particular mucormycosis.Type: ApplicationFiled: September 14, 2012Publication date: May 2, 2013Inventors: Ashraf S. Ibrahim, Mingfu Liu, Teklegiorgis Ghebremariam, Yue Fu, John E. Edwards, Scott Filler
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Publication number: 20130096281Abstract: Provided herein are methods and compositions for use in displaying a polypeptide (e.g., an antibody polypeptide or an antibody polypeptide fragment) on the surface of a yeast cell. Exemplary yeast that can be used in conjunction with various methods and compositions disclosed herein include those of the genus Yarrowia, e.g., Yarrowia lipolytica.Type: ApplicationFiled: January 21, 2011Publication date: April 18, 2013Applicant: OXYRANE UK LIMITEDInventors: Stefan Ryckaert, Guillaume Lerondel
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Patent number: 8372595Abstract: The present invention provides genetically engineered microbial strains, in particular genetically engineered yeast strains, that produce at least 0.5 mg per g CDW of a sphingoid base according to Formula I or a salt or ester thereof. The present invention provides a method to obtain genetically engineered microbial strains producing at least 0.5 mg per g CDW of a sphingoid base according to Formula I or a salt or ester thereof.Type: GrantFiled: May 11, 2007Date of Patent: February 12, 2013Assignee: Evonik Industries AGInventors: Steffen Schaffer, Marco Alexander Van Den Berg, Daniel Boergel, Thomas Hueller
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Publication number: 20130011862Abstract: The present invention relates to processes for the rapid detection, semi-quantification and quantification of live microorganisms in solutions or suspensions using immunomagnetic particles, without requiring pre-enrichment through culture of the microorganism. The invention also relates to kits for carrying out said processes and to the quantification of the microorganisms detected by means of automated biosensor equipment.Type: ApplicationFiled: November 14, 2011Publication date: January 10, 2013Applicant: BIOTICA, BIOQUIMICA ANALITICA, S.L.Inventors: Guillermo RODRIGUEZ ALBALAT, Maria Luisa JIMENEZ BONO, Daniel CANOS MARTI
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Patent number: 8343726Abstract: The present invention provides assays and devices for detection of substances in liquid samples. The assays and devices utilize passive diffusion between a porous material and a porous membrane containing a specific binding pair member to enable detection of the substance of interest.Type: GrantFiled: November 23, 2005Date of Patent: January 1, 2013Assignee: Techlab, Inc.Inventors: James H. Boone, David M. Lyerly, Tracy D. Wilkins
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Patent number: 8323917Abstract: The present invention enables screening for compounds that inhibit the transport of GPI-anchored proteins to fungal cell walls, using a simple assay for transacylation to GlcN-PI using membrane fraction expressing GWT1 protein. New antifungal agents can be created that inhibit the synthesis of fungal cell walls and also inhibit adhesion to host cells by inhibiting the transport of GPI-anchored proteins to fungal cell walls.Type: GrantFiled: November 21, 2003Date of Patent: December 4, 2012Assignees: National Institute of Advanced Industrial Science and Technology, Eisai R&D Management Co., Ltd.Inventors: Kappei Tsukahara, Mamiko Tsuchiya, Yoshifumi Jigami, Kenichi Nakayama, Mariko Umemura, Michiyo Okamoto
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Patent number: 8318448Abstract: The use of an antibody to a C. albicans cell wall antigen or to a solubilized phosphopeptidomannan (PPM) fraction of the cell wall of C. albicans, or preferably a combination of an IgG2 antibody to a phosphopeptidomannan (PPM) fraction of the cell wall of C. albicans, and an IgG1 or IgG3 antibody to a C. albicans cell wall antigen in the diagnosis of candidiasis or invasive candidiasis is disclosed. Also diagnostic tests are disclosed.Type: GrantFiled: May 19, 2009Date of Patent: November 27, 2012Assignee: Fungea ABInventors: Inger Mattsby-Baltzer, Nahid Kondori
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Patent number: 8318445Abstract: The present invention relates to methods and systems for labeling, isolating, detecting, and/or enumerating a statistically significant number of biological cells, or other biological analytes of interest, present in a complex matrix sample. The isolation of a biological target of interest from a sample mixture is done by immunomagnetic separation. Upon introduction of the sample within an imaging chamber, the capture complex (biological target-magnetic capture agent) will be attracted by the magnetic field and will lay on the surface of the chamber in the focal plane of the imaging system.Type: GrantFiled: January 7, 2009Date of Patent: November 27, 2012Assignee: Luminex CorporationInventors: Bruce J-C Bernard, Kurt D. Hoffacker, Charles J. Collins
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Publication number: 20120282635Abstract: A method and apparatus for measuring antibody titers in a thin film sample in an automated system which does not require multiple dilutions. The system provides a simple method for creating an in-situ dilution within a sample analysis chamber without the use of any precision fluid-handling components, and further, to use the same principles to provide a wide range of sample dilutions within the chamber so as to obviate the need for additional dilution steps when dealing with samples possibly containing wide ranges of analyte concentrations.Type: ApplicationFiled: July 16, 2012Publication date: November 8, 2012Applicant: ABBOTT POINT OF CARE, INC.Inventors: Stephen C. Wardlaw, Robert A. Levine
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Patent number: 8298781Abstract: The present invention relates to the characterization of odorant receptors. In particular, the present invention relates to the OR7D4 proteins and nucleic acids encoding OR7D4 proteins and cell systems for screening for modulators of OR7D4 receptors. The present invention further provides assays for the detection of OR7D4 polymorphisms and mutations associated with altered olfactory sensation states, as well as methods of screening for therapeutic agents, ligands, and modulators of OR7D4 receptors.Type: GrantFiled: May 8, 2008Date of Patent: October 30, 2012Assignees: Duke University, The Rockefeller UniversityInventors: Hiroaki Matsunami, Andreas Keller, Hanyi Zhuang, Qiuyi Chi, Leslie B. Vosshall
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Publication number: 20120270236Abstract: Nucleic acid and protein sequences relating to a proton channel (HvI) are disclosed. Nucleic acids, vectors, transformed cells, transgenic animals, polypeptides, and antibodies relating to the HvI gene and protein are disclosed. Also provided are methods of identifying modulators of HvI activity, methods of geno typing subjects with respect to HvI, and methods of diagnosing and treating HvI-mediated disorders.Type: ApplicationFiled: February 15, 2007Publication date: October 25, 2012Applicant: CHILDREN'S MEDICAL CENTER CORPORATIONInventors: Ian S. Ramsey, Magdalene M. Moran, Jayhong A. Chong, David Clapham
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Publication number: 20120237953Abstract: Sucralose-binding TAS2R bitter taste receptors have been identified. Novel methods to identify modulators and in particular inhibitors to the bitter taste of sucralose, and an inhibitor, are provided.Type: ApplicationFiled: May 26, 2012Publication date: September 20, 2012Applicant: Givaudan S.A.Inventors: NICOLE ERNA IRENE BRUNE, JAY PATRICK SLACK, IOANA MARIA UNGUREANU, KIMBERLY GRAY, CHRISTOPHER TODD SIMONS, JENNY ELLEN EVANS PENNIMPEDE
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Publication number: 20120202191Abstract: A method for detecting the presence of a diagnostic moiety indicative of exposure to an infectious organism in a biological sample taken from a human or animal, said method comprising use of a first and second fluorescently labelled reagent which are capable of binding to a diagnostic moiety or to a binding partner in competition with a diagnostic moiety, wherein labels on the first and second labelled reagents act as fluorescent donors and acceptors to one another, the proximity of the reagents to one another being detectable by measuring the emission of fluorescent energy from at least one of the labels.Type: ApplicationFiled: March 14, 2012Publication date: August 9, 2012Applicant: THE SECRETARY OF STATE FOR ENVIRONMENT, FOOD & RURAL AFFAIRSInventor: John McGiven
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Patent number: 8221987Abstract: The present invention relates to the discovery of a novel haplotype of the human taste receptor hT2R50 in the T2R taste receptor family that responds to particular bitter ligands, i.e., 2-acetylpyrazine and ethylpyrazine. The present invention also relates to the use of this novel haplotype in assays for identifying ligands that modulate the activation of the hT250 taste receptor. These compounds potentially may be used as additives in foods, beverages and medicinals for modifying (blocking) hT2R50-associated bitter taste.Type: GrantFiled: October 8, 2010Date of Patent: July 17, 2012Assignee: Senomyx, Inc.Inventors: Xiaodong Li, Alexey Pronin, Hong Xu
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Patent number: 8211691Abstract: Cell lines having genetically modified glycosylation pathways that allow them to carry out a sequence of enzymatic reactions, which mimic the processing of glycoproteins in humans, have been developed. Recombinant proteins expressed in these engineered hosts yield glycoproteins more similar to their human counterparts. The lower eukaryotes, which ordinarily produce high-mannose containing N-glycans, including unicellular and multicellular fungi are modified to produce N-glycans such as Man5GlcNAc2 or other structures along human glycosylation pathways.Type: GrantFiled: October 30, 2007Date of Patent: July 3, 2012Assignee: Glycofi, Inc.Inventor: Tillman U. Gerngross
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Publication number: 20120156695Abstract: The invention relates to an isolated, genetically modified, living non-mammal organism, having increased HMG-CoA-reductase activity compared to the wild type, and having reduced C24-methyltransferase and/or delta22-desaturase activity compared to the wild type. The invention is characterized in that the organism has increased dehydrocholesterol-delta70-reductase activity compared to the wild type. The invention further relates to different uses of such an organism, to a test kit comprising such an organism, and to a membrane extract of such an organism.Type: ApplicationFiled: April 1, 2010Publication date: June 21, 2012Applicant: ORGANOBALANCE GMBHInventors: Michael Schilling, Christine Lang, Andreas Raab
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Publication number: 20120129157Abstract: The invention provides methods, devices and kits for rapid detection and identification of one or more live target microorganisms in a liquid sample or grown on plates containing nutrient media. The invention includes mixing one or more target microorganisms with one or more aptamers and/or one or more antibodies, each conjugated to a reporter compound and specific for a first site on one or more target microorganisms to form a mixture. The mixture is placed on a permeable membrane having immobilized thereon one or more aptamers linked to an amine compound, and/or one or more antibodies, each specific for a second site on one or more target microorganisms or a site on the aptamer conjugate and/or antibody conjugate. A detection solution is added to the membrane and detection and identification of one or more target microorganisms is achieved in about one hour or less.Type: ApplicationFiled: January 13, 2012Publication date: May 24, 2012Inventors: Bret T. Barnhizer, Sergey Gazenko
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Publication number: 20120122736Abstract: A sensor comprising an electronic circuit electrically coupled to a type III-V semiconductor material, for example indium arsenide (InAs) and an antibody contacting the type III-V semiconductor material. The sensor produces measurable N changes in the electrical properties of the semiconductor upon antibody-antigen binding events. Electrical properties measurable by the electronic device may include resistivity, capacitance, impedance, and inductance. A method of detecting an antigen using sensors of the invention. A method of detecting a reaction of an analyte to a stimulus using sensors of the invention. Sensor arrays comprising multiple sensors of the invention.Type: ApplicationFiled: February 11, 2010Publication date: May 17, 2012Applicant: DUKE UNIVERSITYInventors: R. Michael Angelo, April S. Brown, Scott Wolter, William V. Lampert
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Patent number: 8158405Abstract: A method of treating a liquid sample having microbiological target species therein to concentrate the species and collect lysate is disclosed. The liquid sample comprises non-target microbiological particles, inorganic particles, and microbiological target species. The liquid is passed through a prefilter medium to allow the target species to pass through as filtrate and retain non-target microbiological products and inorganic particles thereon. The filtrate is contacted with a main filtration medium adapted to retain the target species thereon as retentate. The retentate is lysed to form a lysate containing target material that was enveloped within the microbiological target species. The microbiological species may comprise cell containing or viral material. Target materials comprise intracellular nucleic acids, or in the case of viral sampling, nucleic acids encased within the protein sheath or coating of the virus.Type: GrantFiled: June 30, 2008Date of Patent: April 17, 2012Assignee: General Electric CompanyInventors: Scott Boyette, Rong Xu, Jing Luo, Weiqing Xu, Jing Chen, Weimin Xiao, Tong Chen
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Publication number: 20120070850Abstract: It is possible to determine the presence of bacteria in a sample solution in a shorter period of time without changing a conventional incubating method. Bacteria in a sample solution are incubated in, for example, a sterilized agar medium 10 having a layer thickness of 0.1 ?m to 1 ?m formed on an electrode of a crystal resonator 2, and an oscillation frequency is measured. When the bacteria proliferate, the mass of the entire crystal resonator 2 increases, and the oscillation frequency decreases. Therefore, by monitoring presence of such a change over time, presence of bacteria in the sample solution can be determined quickly.Type: ApplicationFiled: November 16, 2011Publication date: March 22, 2012Applicant: NIHON DEMPA KOGYO CO., LTD.Inventors: Mitsuaki Koyama, Shunichi Wakamatsu, Wakako Shinobu
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Publication number: 20120064093Abstract: The invention relates to antibodies to Aspergillus species and to methods of producing those antibodies. The invention also relates to the use of such antibodies in identifying the presence of the Aspergillus species and to methods of treating an infection with the Aspergillus species.Type: ApplicationFiled: January 18, 2010Publication date: March 15, 2012Inventor: Christopher Thornton
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Patent number: 8133679Abstract: Methods of screening candidate agents to identify lead compounds for the development of therapeutic agents for the treatment of a neurodegenerative disease, such as Huntington's Disease and Parkinson's Disease and methods for identifying a mutation in, or changes in expression of, a gene associated with neurodegenerative disease, such as Huntington's Disease and Parkinson's Disease, are provided.Type: GrantFiled: November 17, 2008Date of Patent: March 13, 2012Assignees: Whitehead Institute for Biomedical Research, University of WashingtonInventors: Paul J. Muchowski, Susan L. Lindquist, Tiago Outeiro
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Publication number: 20120058494Abstract: The present invention presents designs for high extinction quenched “dyedrons” that can be activated by conversion of a single acceptor/quencher in the molecular assembly to a fluorescent state. The quencher is activated by noncovalent binding to a unique complementary expressible fluorogen activating peptide (FAP). In this way, the quencher serves as the homogeneous switch, receiving energy efficiently from each of the donor molecules of the dendronic antenna, and releasing it as fluorescence only when activated by binding. The sum of the extinction of the multiple dyes on the antenna will provide dramatic enhancements in the effective brightness of the probe in standard imaging systems. This approach provides a set of probes with exceptional brightness, specifically targeted to an expressed tag that activates the fluorescence of the dyedron.Type: ApplicationFiled: February 16, 2010Publication date: March 8, 2012Applicant: CARNEGIE MELLON UNIVERSITYInventors: Marcel P. Bruchez, Lauren A. Ernst, James Fitzpatrick, Chris Szent-Gyorgyi, Brigitte F. Schmidt, Alan Waggoner
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Publication number: 20120003661Abstract: Methods and devices are provided for the rapid and specific detection of target microorganisms, cells, and the like. In one embodiment, the methods involve contacting a target microorganism (e.g., in a sample) with a permeabilization reagent that selectively permeabilizes or lyses the microorganism; contacting the selectively permeabilized microorganism with a detection reagent that is taken into the selectively permeabilized organism or that contacts metabolites or enzymes released by the selectively permeabilized microorganism, where the detection reagent produces a signal in the presence of said metabolites or enzymes; and detecting a signal produced by the detection reagent in the presence of the metabolites or enzymes wherein the strength of the signal indicates the presence and/or amount of the target microorganism in the sample.Type: ApplicationFiled: June 27, 2011Publication date: January 5, 2012Applicant: C3 JIAN, INC.Inventors: Randal H. Eckert, Chris Kaplan, Jian He, Daniel K. Yarbrough, Maxwell Anderson, Jee-Hyun Sim
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Publication number: 20110311991Abstract: Steviol glycosides have been discovered to bind the bitter taste receptor TAS2R44. Novel methods of identifying modulators and in particular inhibitors to the bitter taste of steviol glycosides and an inhibitor of steviol glycosides are provided.Type: ApplicationFiled: June 11, 2009Publication date: December 22, 2011Applicant: Givaudan SAInventors: Jay Patrick Slack, Jenny Ellen Evans Pennimpede
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Patent number: 8071326Abstract: A rapid method for the quantitation of various live cell types is described. This new cell fluorescence method correlates with other methods of enumerating cells such as the standard plate count, the methylene blue method and the slide viability technique. The method is particularly useful in several applications such as: a) quantitating bacteria in milk, yogurt, cheese, meat and other foods, b) quantitating yeast cells in brewing, fermentation and bread making, c) quantitating mammalian cells in research, food and clinical settings. The method is especially useful when both total and viable cell counts are required such as in the brewing industry. The method can also be employed to determine the metabolic activity of cells in a sample. The apparatus, device, and/or system used for cell quantitation is also disclosed.Type: GrantFiled: February 11, 2011Date of Patent: December 6, 2011Assignee: Genprime, Inc.Inventors: James E. Fleming, Jason Buck Somes, Darby McLean, Jerad R. Holcomb
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Patent number: 8063026Abstract: Provided herein are methods of using mannan oligosaccharides, to treat, palliate, relieve, prevent and/or eliminate urinary tract infections of humans. A mixture of oligosaccharides derived from yeast cell walls is shown to reduce, eliminate and prevent the symptoms of UTI in patients.Type: GrantFiled: October 6, 2006Date of Patent: November 22, 2011Inventor: Richard Katz
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Publication number: 20110281283Abstract: The present invention relates to animal feed additives and detection thereof in feed products. Additionally, the present invention relates to yeast cell wall components, their methods of isolation, and compositions and methods for the immunological detection thereof.Type: ApplicationFiled: May 13, 2011Publication date: November 17, 2011Applicant: ALLTECH, INC.Inventors: Colm Moran, Stefan Kwiatkowski, Alexandros Yiannikouris, Ursula Anne Thielen
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Publication number: 20110262893Abstract: The invention generally relates to using magnetic particles and alternating magnet fields to separate a target analyte from a sample. In certain embodiments, methods of the invention involve contacting a sample with magnetic particles including first moieties specific for a target analyte, thereby forming target/particle complexes in the sample, flowing the sample through a channel including second moieties attached to at least one surface of the channel, applying alternating magnetic fields to the flowing sample to result in target/particle complexes being brought into proximity of the surface to bind the second moieties and unbound particles remaining free in the sample, binding the target/particle complexes to the second moieties, and washing away unbound particles and unbound analytes of the sample.Type: ApplicationFiled: August 12, 2010Publication date: October 27, 2011Applicant: NanoMR, Inc.Inventors: Sergey A. Dryga, Victor C. Esch, Richard G. Saul, Andrew F. McDowell
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Patent number: 8039206Abstract: Improved methods for detecting microorganisms, such as yeast and bacteria in mixtures, are disclosed. Methods include passing a sample mixture through a filter device, which has been pretreated with a detergent, resuspending the filtrand in the filter membranes and detecting the presence of microorganisms in the filtrand.Type: GrantFiled: August 7, 2000Date of Patent: October 18, 2011Assignee: Millipore CorporationInventor: Elizabeth Ann Keenan
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Patent number: 8039217Abstract: Genomic actions and/or proteomic interactions for pathophysiological processes and for physiological processes are determined at associated redox state conditions. Protein interactions are correlated with oxygen tensions. Identification of markers for disease including epitopes is effected in the presence of simulated redox state perturbations. Screening for previously unknown receptors and activating ligands is carried out in the presence of alteration of redox state.Type: GrantFiled: February 26, 2008Date of Patent: October 18, 2011Assignee: Duke UniversityInventor: Jonathan S. Stamler
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Publication number: 20110223618Abstract: This invention relates to chimeric taste receptors comprising the extracellular portion of one T1R or a variant or fragment thereof, either T1R1 or T1R2, and the transmembrane portion of another T1R or a variant or fragment thereof, either T1R1 or T1R2, preferably associated with a T1R3 polypeptide and a suitable G protein. These chimeric taste receptors and cells which express such chimeric taste receptors are useful in assays for identifying sweet and umami ligands as well in assays for identifying sweet and umami enhancers. Additionally, these chimeric taste receptors and cells which express same can be used to map and determine where specific sweet and umami ligands interact with their respective receptors and to elucidate the mechanism of receptor activation.Type: ApplicationFiled: February 3, 2011Publication date: September 15, 2011Inventors: Xiaodong Li, Feng Zhang, Hong Xu, Qing Li
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Publication number: 20110223620Abstract: It is possible to determine the presence of bacteria in a sample solution in a shorter period of time without changing a conventional incubating method. Bacteria in a sample solution are incubated in, for example, a sterilized agar medium 10 having a layer thickness of 0.1 ?m to 1 ?m formed on an electrode of a crystal resonator 2, and an oscillation frequency is measured. When the bacteria proliferate, the mass of the entire crystal resonator 2 increases, and the oscillation frequency decreases. Therefore, by monitoring presence of such a change over time, presence of bacteria in the sample solution can be determined quickly.Type: ApplicationFiled: February 10, 2011Publication date: September 15, 2011Applicant: NIHON DEMPA KOGYO CO., LTD.Inventors: Mitsuaki Koyama, Shunichi Wakamatsu, Wakako Shinobu
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Patent number: 7999090Abstract: The present invention provides isolated DNA encoding a GWT1 protein having activity to confer resistance of a fungus against a compound of formula Ia, and wherein a defect of a function of the GWT1 protein leads to a decrease in the amount of a glycosylphosphatidylinositol (GPI)-anchored protein in the cell wall of a fungus.Type: GrantFiled: April 15, 2008Date of Patent: August 16, 2011Assignee: Eisai Co., LtdInventors: Kappei Tsukahara, Katsura Hata, Koji Sagane, Kazutaka Nakamoto, Mamiko Tsuchiya, Naoaki Watanabe, Fuminori Ohba, Itaru Tsukada, Norihiro Ueda, Keigo Tanaka, Junko Kai
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Publication number: 20110165555Abstract: The present invention is directed to satisfying the need to detect microbial contamination of food products. The described bioseparator/bioreactor coupled with an optical/electrochemical biosensor was able to specifically detect E. coli O157:H7 from 8.8×101 to 8.8×106 CFU/ml in 2.5 hours without any enrichment. Using this invention, concentrations of S. Typhimurium ranging from 8.6×102 to 8.6×106 CFU/ml in pure culture were detected in 2 hours without any enrichment. The invention may also be used for the detection of S. Seftenberg, which has the same sensitivity as S. Typhimurium. Other pathogens such as L. monocytogenes and S. Heidleberg did not interfere with the detection. The optimum inner diameter of the 25 cm long column for the detection of E. coli O157:H7 is 250 ?m.Type: ApplicationFiled: December 13, 2010Publication date: July 7, 2011Applicant: BOARD OF TRUSTEES OF THE UNIVERSITY OF ARKANSASInventors: Yanbin Li, Yongcheng Liu
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Publication number: 20110143374Abstract: TAS2R46 was identified as a dextromethorphan-binding bitter taste receptor. Novel methods to identify modulators and in particular inhibitors to the bitter taste of dextromethorphan are provided.Type: ApplicationFiled: August 6, 2009Publication date: June 16, 2011Inventors: Jay Patrick Slack, Jenny Ellen Evans Pennimpede