Bacteria Or Actinomycetales Patents (Class 435/7.32)
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Publication number: 20110165555Abstract: The present invention is directed to satisfying the need to detect microbial contamination of food products. The described bioseparator/bioreactor coupled with an optical/electrochemical biosensor was able to specifically detect E. coli O157:H7 from 8.8×101 to 8.8×106 CFU/ml in 2.5 hours without any enrichment. Using this invention, concentrations of S. Typhimurium ranging from 8.6×102 to 8.6×106 CFU/ml in pure culture were detected in 2 hours without any enrichment. The invention may also be used for the detection of S. Seftenberg, which has the same sensitivity as S. Typhimurium. Other pathogens such as L. monocytogenes and S. Heidleberg did not interfere with the detection. The optimum inner diameter of the 25 cm long column for the detection of E. coli O157:H7 is 250 ?m.Type: ApplicationFiled: December 13, 2010Publication date: July 7, 2011Applicant: BOARD OF TRUSTEES OF THE UNIVERSITY OF ARKANSASInventors: Yanbin Li, Yongcheng Liu
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Patent number: 7972773Abstract: The invention is directed to a method for detecting low concentrations of bacteria in liquid solution that may or may not be complex liquid solutions. In one embodiment, immunomagnetic separation (IMS) is used to separate target bacterium that may be in a liquid mixture from other constituents in the mixture. A low concentration of a bacteriophage for the target bacteria is subsequently used to infect target bacterial cells that have been captured using the IMS technique. If at least a certain concentration of target bacterium are present, the bacteriophage will multiply to a point that is detectable. Matrix assisted laser desorption ionization/time-of-flight-mass spectrometry (MALDI/TOF-MS) is then used to produce a mass spectrum that is analyzed to determine if one or more proteins associated with the bacteriophage are present, thereby indirectly indicating that target bacterium were present in the liquid mixture.Type: GrantFiled: January 23, 2007Date of Patent: July 5, 2011Assignee: Colorado School of MinesInventors: Angelo J. Madonna, Kent J. Voorhees, Jon C. Rees
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Publication number: 20110159477Abstract: The present invention describes product and analysis method for analysis of a substance, a virus, a bacteria or a cell which binds to carbohydrate structures. One or more carbohydrates, or carbohydrate derivatives, is bound to spherical or non-spherical polymer beads (for example microspheres or nanospheres). Formed carbohydrate-polymer beads or carbohydrate-polymer particles, can bind to with more or less specific affinity to the substance, bacteria, virus or cell which shall be detected. The carbohydrate beads is contacted with a sample containing the substance, bacteria, virus or cell (completely or partially isolated, or not purified, in a non-diluted or diluted solution). The resulting mixture is optionally added to a gel, or a column containing a gel, through which the resulting mixture (of carbohydrate beads or carbohydrate particles bound to the substance, virus, bacteria or cell), is allowed to migrate. Detection of aggregates is made visually or using apparatus for detection.Type: ApplicationFiled: June 10, 2009Publication date: June 30, 2011Inventors: Kurt Nilsson, Åsa Kronblad
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Publication number: 20110150889Abstract: The invention described herein relates to a Haemophilus influenzae (H. influenzae) regulon encoding type IV pili. In particular, the invention relates to type pili from nontypeable H. influenzae (NTHi) and from H. influenzae strains a, b, c, e and f. The invention provides isolated H. influenzae pilus polynucleotides and polypeptides encoded by the polynucleotides as well as polynucleotides and polypeptides encoded by the polynucleotides involved in the assembly/disassembly of the structure. The invention also relates to uses of these polynucleotides and/or polypeptides including methods for eliciting an immune response to H. influenzae and methods of treating and preventing H. influenzae related pathological conditions.Type: ApplicationFiled: January 12, 2011Publication date: June 23, 2011Applicant: NATIONWIDE CHILDREN'S HOSPITAL, INC.Inventors: Lauren O. Bakaletz, Robert S. Munson, JR.
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Patent number: 7964368Abstract: Procedures and means for evaluating effectiveness of bacterial-lethality, following batch-processed containerized food production operations and aseptic-flow food-production operations as containerized in aseptic-containers, in preparing for non-refrigerated marketing are described. The evaluations significantly expedite determining whether thermally-processed containerized food-production is safe for non-refrigerated marketing. The presence or absence of live spore-forming bacteria is determined chemically free of extended storage requirements relying on a mechanical-failure indication of food-spoilage. Also, a biological-indication verification of microbial-biocidal status of the packaged food is made available.Type: GrantFiled: April 25, 2006Date of Patent: June 21, 2011Assignee: SGM Biotech, Inc.Inventors: John R. Gillis, Kurtis J. McCauley
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Publication number: 20110143374Abstract: TAS2R46 was identified as a dextromethorphan-binding bitter taste receptor. Novel methods to identify modulators and in particular inhibitors to the bitter taste of dextromethorphan are provided.Type: ApplicationFiled: August 6, 2009Publication date: June 16, 2011Inventors: Jay Patrick Slack, Jenny Ellen Evans Pennimpede
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Publication number: 20110130305Abstract: Provided are dyes and compositions which are useful in a number of applications, such as the detection and monitoring protein aggregation, kinetic studies of protein aggregation, neurofibrillary plaques analysis, evaluation of protein formulation stability, protein thermal stability shift assay and analysis of molecular chaperone activity. These dyes and compositions are also useful as probes in nucleic acid and protein detection.Type: ApplicationFiled: November 30, 2009Publication date: June 2, 2011Inventors: Wayne Forrest Patton, Sergiy M. Yarmoluk, Praveen Pande, Vladyslava Kovalska, Lijun Dai, Kateryna Volkova, Jack Coleman, Mykhaylo Losytskyy, Anthony Ludlum, Anatoliy Balanda
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Publication number: 20110117539Abstract: There is provided mechanisms for the detection of an analyte in a sample. The mechanisms utilize at least a first measurement channel comprising a detection reactant corresponding to the analyte to be detected, and at least a microstructure associated with the first measurement channel. When the mechanisms are in use, the sample is introduced into the first measurement channel and propagated by way of the first measurement channel towards the microstructure. If the analyte, if it is present in the sample, the analyte interacts with the detection reactant to form a networked product, and the microstructure is configured to filter the networked product.Type: ApplicationFiled: October 30, 2008Publication date: May 19, 2011Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATIONInventors: Emmanuel Delamarche, Daniel J. Solis
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Publication number: 20110117581Abstract: The invention is directed to methods and devices for reducing interference from leukocytes in an competitive analyte immunoassays. In one embodiment, the invention is to a method comprising the steps of (a) amending a biological sample such as a whole blood sample with sacrificial beads opsonized for leukocytes; and (b) performing a competitive immunoassay on the amended sample to determine the concentration of said analyte in said sample. Preferably, the sample is amended with IgG-coated sacrificial beads.Type: ApplicationFiled: November 17, 2009Publication date: May 19, 2011Applicant: Abbott Point of Care Inc.Inventors: John Lewis Emerson Campbell, Graham Davis
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Publication number: 20110117580Abstract: The invention is directed to methods and devices for reducing interference from leukocytes in an analyte immunoassay, and in particular in non-competitive immunoassays. In one embodiment, the invention is to a method comprising the steps of (a) amending a biological sample such as a whole blood sample with sacrificial beads; and (b) performing a non-competitive immunoassay on the amended sample to determine the concentration of said analyte in said sample. Preferably, the sample is amended with IgG-coated sacrificial beads.Type: ApplicationFiled: November 17, 2009Publication date: May 19, 2011Applicant: Abbott Point of Care Inc.Inventors: John Lewis Emerson Campbell, Adam Roger Moss, Graham Davis
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Publication number: 20110111390Abstract: Method for in vitro detection and/or quantification and/or identification of infectious compounds present in a fluid medium M constituting a biological material, in which method a suspension of microbeads of solid polymer material capable of binding proteins is prepared; the microbeads are loaded with ?2GPI proteins by coupling with a sufficient amount of ?2GPI proteins; said microbeads are brought into contact with the fluid medium M while adding ions of at least one oxidizing metal, so as to bind the infectious compounds to the ?2GPI proteins; the microbeads thus prepared are separated from their suspension medium, so as to obtain a residue; and the infectious compounds of the residue are detected and/or quantified and/or identified.Type: ApplicationFiled: January 30, 2009Publication date: May 12, 2011Applicant: APOH Technologies SAInventor: Ilias Stefas
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Patent number: 7939275Abstract: This invention relates to assays for Chlamydia which include the step of inactivating lipid oxidation activity in a biological sample, such as a blood or serum sample. This inactivation improves the detection of Chlamydia antigens or anti-Chlamydia antibodies. Methods and materials for the detection of Chlamydia and Chlamydial infection are provided.Type: GrantFiled: February 24, 2006Date of Patent: May 10, 2011Assignee: Cambridge Theranostics LimitedInventor: Ivan Petyaev
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Publication number: 20110104721Abstract: Lactobacillus screening methods were carried out using surface plasmon resonance spectrums and human intestinal mucin and blood group antigens as probes. A trial to set selection criteria in the above-mentioned methods of screening for lactobacilli was made to adapt the methods to mass screening, and it was discovered that lactobacilli compatible with ABO blood groups can be screened by setting 100 RU as a criterion for judging bacterial binding under certain conditions. Using 238 lactobacillus strains, the above-mentioned screening methods and tests to judge their compatibility for the use of yogurt production were carried out, to at long last specifically discover bacillus strains compatible with blood groups A, B, and O.Type: ApplicationFiled: January 10, 2011Publication date: May 5, 2011Inventors: Tadao Saito, Yasushi Kawai, Hideaki Uchida, Katsunori Kimura, Kakuhei Isawa, Keisuke Furuichi
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Publication number: 20110104282Abstract: The invention provides for new methods for treatment and diagnosis of irritable bowel syndrome (IBS). In particular, there is disclosed the use of a Chlamydia activating agent and one or several antibiotics selected from macroildes and benzoxazinorifamycins in the preparation of combination agent for simultaneous, concomitant or sequential administration for the treatment of IBS.Type: ApplicationFiled: April 27, 2009Publication date: May 5, 2011Inventors: Greger Lindberg, Aldona Dlugosz
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Patent number: 7935520Abstract: Methods and compositions are provided for detecting biomolecular interactions. The use of labels is not required and the methods can be performed in a high-throughput manner. The invention also provides optical devices useful as narrow band filters.Type: GrantFiled: June 25, 2009Date of Patent: May 3, 2011Assignee: SRU Biosystems, Inc.Inventors: Brian T. Cunningham, Jane Pepper, Bo Lin, Peter Li, Homer Pien, Jean Qiu
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Publication number: 20110097741Abstract: A novel sweet receptor protein, corresponding nucleic acid sequence, expression vectors, transfected host cells, and screening methods for modulators including ligands of the sweet taste response employing the aforementioned are provided.Type: ApplicationFiled: April 19, 2006Publication date: April 28, 2011Inventor: Jay Patrick Slack
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Publication number: 20110097745Abstract: The invention provides means and methods for determining the presence of infecting microbes in a liquid. The method comprises steps of introducing the liquid to the sample container of a microbial analyzer, the sample container further containing nutrients. Other steps include carrying out a first incubation of said specimen, adding bioluminescent tester microbes to the specimen, carrying out a second incubation of the specimen and measuring bioluminescence, thereby determining said presence and/or concentration of infecting microbes.Type: ApplicationFiled: August 11, 2008Publication date: April 28, 2011Applicant: CHECK LIGHT LTD.Inventor: Nirit Ulitzur
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Publication number: 20110097706Abstract: The invention relates to a micro-reactor for observing small particles, cells, bacteria, viruses or protein molecules in a fluid. The micro-reactor shows a first channel formed between two layers for containing the fluid, with an inlet and an outlet, the two layers separated by a first distance. A likewise second channel with an inlet and an outlet is placed adjacent to the first channel. A gap connects the first channel and the second channel, at the gap at least one layer showing a window transparent to the method of inspection and at the window the two layers being separated by a very small distance of, for example, 1 ?m or less. The micro-reactor may be used with an optical microscope (in which all particles are in focus), inspection with a Scanning Transmission Electron Microscope (in which the range of the electrons is limited), inspection with soft X-rays in the 250-500 eV range (also showing a limited range), etc.Type: ApplicationFiled: October 26, 2010Publication date: April 28, 2011Applicants: FEI COMPANY, STICHTING HET NEDERLANDS KANKER INSTITUUTInventors: Gerard Anne Nicolaas van Veen, Jacobus Peter Johannes Peters, Pleun Dona, Alan Frank De Jong
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Publication number: 20110098214Abstract: A lipopolysaccharide (LPS) and/or lipid A binding agent is provided. The LPS and/or lipid A binding agent contains an LPS and/or lipid A binding peptide, such as a peptide comprising an amino acid sequence of XYSSS (X=K, R, or H), or a derivative thereof, as an active ingredient. The LPS and/or lipid A binding agent is useful as, for example, an LPS and/or lipid A neutralizing agent or an LPS and/or lipid A removing agent.Type: ApplicationFiled: December 3, 2010Publication date: April 28, 2011Applicant: PEPTIDE DOOR CO., LTD.Inventors: MEGUMI MATSUMOTO, MASATSUGU SUZUKI
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Patent number: 7932051Abstract: The present invention provides a test medium comprising a test microorganism, an indicator and a metal in ionized form, wherein the valence of said metal is at least 2 and the concentration of said metal is between 0.001 M and 1 M. Furthermore, there is provided a method for the determination of the presence of an antibiotic characterized in that a metal salt is added to said test medium and/or to said sample of fluid. Finally, there is provided a kit suitable for the determination of an antibiotic in a fluid.Type: GrantFiled: September 6, 2004Date of Patent: April 26, 2011Assignee: R-Biopharm AGInventors: Edith Magda Lucia Geijp, Jacobus J. Stark
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Patent number: 7927818Abstract: Contemplated compositions, devices, and methods are drawn to various antigens from the pathogen M. tuberculosis and their use in vaccines, therapeutic agents, and various diagnostic tests. In particularly preferred aspects, the antigens are immunodominant and have quantified and known relative reactivities with respect to sera of a population infected with the pathogen, and/or have a known association with a disease parameter.Type: GrantFiled: May 13, 2009Date of Patent: April 19, 2011Inventors: Philip Felgner, Xiaowu Liang, Maria Laura Gennaro
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Publication number: 20110085990Abstract: This, intention relates to methods of assessing the biofiora of the mouth and of providing appropriate treatment utilizing a basic amino acid in accordance with the assessment.Type: ApplicationFiled: February 6, 2009Publication date: April 14, 2011Inventors: Richard Scott Sullivan, Laurence Du Thumm, Yanan Hu, Stacey Lavender, Ralph Peter Santarpia, III, Zhiqiang Liu
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Publication number: 20110086369Abstract: The present invention describes novel fluorescent compounds analogous to alkyl phospholipids, containing highly photostable fluorescent groups in the structure thereof and emitting in the visible spectrum wavelength zone. Said compounds are easily introduced to microorganisms, enabling the detection and identification of these organisms by means of fluorescence microscopy. The novel fluorescent compounds can also diagnose, rapidly and easily, the presence of pathogenic microorganisms characterised in that they are resistant to treatment with alkyl phospholipids.Type: ApplicationFiled: March 30, 2009Publication date: April 14, 2011Inventors: A. Ulises Acuña Fernández, Francisco Amat Guerri, Eugenia Carrillo Gallego, Valentín Hornillos Gomez Recuero, Susana Marcos Celestino, José María Requejo Isidro, Luis Ignacio Rivas López, José María Saugar Cruz, Carmen Del Aguila De La Puente, Jesús Merayo Lloves
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Publication number: 20110076670Abstract: A first set of antibodies are bonded to a substrate, and are exposed to and bonded with target antigens. A second set of antibodies are bonded to nanoparticles, and the nanoparticle labeled antibodies are exposed to the targeted antigens. An electromagnetic write-head magnetizes the nanoparticles, and then a read-sensor detects the freshly magnetized nanoparticles. The substrate comprises a flexible film or a Peltier material to allow selective heating and cooling of the antigens and antibodies. Nanoparticles of different magnetic properties may be selectively paired with antibodies associated with different antigens to allow different antigens to be detected upon a single scan by the read-sensor.Type: ApplicationFiled: September 22, 2010Publication date: March 31, 2011Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATIONInventors: Dylan Joseph Boday, Lee Curtis Randall, Stephen Leonard Schwartz, Anna W. Topol, Daniel James Winarski
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Publication number: 20110076699Abstract: Disclosed is the cloning, expression and purification of a hemolysin protein and its protein fragments in Anaplasma phagocytophilum. The recombinant hemolysin and its protein fragments are useful in the ELISA detection of anaplasma pathogen. The use of same as a kit for ELISA is also disclosed.Type: ApplicationFiled: February 9, 2010Publication date: March 31, 2011Applicant: MEDICAL DIAGNOSTIC LABORATORIES, LLCInventors: John G. Hoey, Denise P. Dimitrov, Lisa P. Huang, Martin E. Adelson, Eli Mordechai
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Patent number: 7910063Abstract: A device for detection of magnetic permeability ? or, alternatively, relative magnetic permeability ?r or, alternatively, relative magnetic susceptibility (?r?1) of a sample, wherein said device contains a sample chamber and at least two coils, one coil surrounding said sample chamber and one coil placed so as to be in thermal contact by being physically connected to the material which constitutes the sample chamber, but without surrounding the cavity of the sample chamber, said sample chamber having at least one opening for introduction of a sample or a sample container holding a sample, said device also provided with an electronic circuit which measures the difference in inductance between the two coils.Type: GrantFiled: March 7, 2003Date of Patent: March 22, 2011Assignee: Lifeassays ABInventor: Dario Kriz
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Publication number: 20110065090Abstract: A method of detection comprising a conjugate of a randomly and asymmetrically branched dendritic polymer.Type: ApplicationFiled: July 12, 2010Publication date: March 17, 2011Applicant: ANP TECHNOLOGIES, INC.Inventors: Ray Yin, Dujie Qin, Jing Pan
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Publication number: 20110065132Abstract: Antibodies, polypeptides, and polynucleotides are provided for the detection, prevention, amelioration and treatment of diseases caused by Actinobacillus actinomycetemcomitans.Type: ApplicationFiled: November 22, 2010Publication date: March 17, 2011Inventors: MARTIN HANDFIELD, JEFFREY DANIEL HILLMAN, ANN PROGULSKE-FOX
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Patent number: 7906296Abstract: The invention provides compositions and methods for the detection of Anaplasma platys polynucleotides and polypeptides.Type: GrantFiled: February 10, 2009Date of Patent: March 15, 2011Assignee: IDEXX Laboratories, Inc.Inventors: Melissa Jane Beall, Phyllis Ione Tyrrell, Ramaswamy Chandrashekar, Jiayou Liu
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Patent number: 7901898Abstract: A method of diagnosing in a host infection by or exposure to a mycobacterium which expresses ESAT-6 comprising (i) contacting a population of T cells from the host with one or more peptides or analogues selected from the peptides represented by SEQ ID NO:1 to 11 and analogues thereof which can bind a T cell receptor which recognises any of the said peptides, and (ii) determining whether the T cells of said T cell population recognise the peptide(s) and/or analogue(s). The method may performed in vivo. Peptides and a kit which enable the method to be carried out are provided.Type: GrantFiled: October 14, 2009Date of Patent: March 8, 2011Assignee: Isis Innovation LimitedInventors: Ajit Lalvani, Ansar Pathan
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Publication number: 20110053184Abstract: The present invention relates to the discovery of a novel haplotype of the human taste receptor hT2R50 in the T2R taste receptor family that responds to particular bitter ligands, i.e., 2-acetylpyrazine and ethylpyrazine. The present invention also relates to the use of this novel haplotype in assays for identifying ligands that modulate the activation of the hT250 taste receptor. These compounds potentially may be used as additives in foods, beverages and medicinals for modifying (blocking) hT2R50-associated bitter taste.Type: ApplicationFiled: October 8, 2010Publication date: March 3, 2011Inventors: Xiaodong Li, Alexey Pronin, Hong Xu
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Patent number: 7892552Abstract: This invention provides isolated nucleic acids encoding polypeptides comprising amino acid sequences of streptococcal matrix adhesion (Ema) polypeptides. The invention provides nucleic acids encoding Group B streptococcal Ema polypeptides EmaA, EmaB, EmaC, EmaD and EmaE. The present invention provides isolated polypeptides comprising amino acid sequences of Group B streptococcal polypeptides EmaA, EmaB, EmaC, EmaD and EmaE, including analogs, variants, mutants, derivatives and fragments thereof. Ema homologous polypeptides from additional bacterial species, including S. pneumoniae, S. pyogenes, E. faecalis and C. diptheriae are also provided. Antibodies to the Ema polypeptides and immunogenic fragments thereof are also provided. The present invention relates to the identification and prevention of infections by virulent forms of streptococci.Type: GrantFiled: December 7, 2009Date of Patent: February 22, 2011Assignees: University of Utah Research Foundation, St. Jude Children's Research HospitalInventors: Elisabeth Adderson, John Bohnsack
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Publication number: 20110039280Abstract: A hybrid microfluidic biochip designed to perform multiplexed detection of singled- celled pathogens using a combination of SPR and epi-fluorescence imaging. The device comprises an array of gold spots, each functionalized with a capture biomolecule targeting a specific pathogen. This biosensor array is enclosed by a polydimethylsiloxane (PDMS) microfluidic flow chamber that delivers a magnetically concentrated sample to be tested. The sample is imaged by surface plasmon resonance on the bottom of the biochip, and epi- fluorescence on the top.Type: ApplicationFiled: October 29, 2008Publication date: February 17, 2011Applicant: PURDUE RESEARCH FOUNDATIONInventors: James F. Leary, Kinam Park, Ghanashyam Acharya, Michael Zordan
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Publication number: 20110039256Abstract: A method for detecting the presence of a diagnostic moiety indicative of exposure to an infectious organism in a biological sample taken from a human or animal, said method comprising; (a) adding to said sample a first fluorescently labelled reagent which binds said diagnostic moiety, and a second fluorescently labelled reagent which either binds said diagnostic moiety in addition to said first fluorescently labelled reagent, or which binds the first fluorescently labelled reagent or a complex comprising the first fluorescently labelled reagent in competition to the said diagnostic moiety, wherein a label on one of the first or second fluorescently labelled reagent acts as a fluorescent energy donor compound and wherein the other of the first or second fluorescently labelled reagent acts as a fluorescent energy acceptor compound which is able to accept fluorescent energy from said donor compound; (b) exciting the fluorescent energy donor compound by illuminating with light of a wavelength which is absorbed byType: ApplicationFiled: March 27, 2009Publication date: February 17, 2011Applicant: THE SECRETARY OF STATE FOR ENVIRONMENT, FOOD, & RURAL AFFAIRSInventor: John McGiven
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Patent number: 7888054Abstract: The invention provides Ehrlichia canis antigens that can be used to detect E. canis infected animals regardless of whether the animals have been vaccinated for E. canis. The invention also provides compositions and methods for determining the presence of E. canis antigens and antibodies.Type: GrantFiled: October 31, 2008Date of Patent: February 15, 2011Assignee: IDEXX Laboratories, Inc.Inventors: Eugene Regis Krah, III, Melissa Beall, Thomas Patrick O'Connor, Jr., Ramaswamy Chandrashekar
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Patent number: 7887815Abstract: The present invention relates, e.g., to an isolated peptide consisting of the sequence MKKDDQIAAAIALRGMA (SEQ ID NO:1) or an active variant thereof, wherein the peptide or active variant can bind specifically to an antibody induced by a causative agent of Lyme disease (a pathogenic Borrelia), e.g. in a sample from a subject having Lyme disease. Also disclosed are linear multimeric peptides that contain the peptide represented by SEQ ID NO:1 as well as one or more additional peptide epitopes from other Borrelia proteins that can also bind specifically to an antibody as above. Compositions and diagnostic kits comprising a peptide of the invention are described, as are diagnostic assays using the peptide(s).Type: GrantFiled: November 10, 2008Date of Patent: February 15, 2011Assignee: Biopeptides CorporationInventors: Raymond J. Dattwyler, Maria Gomes-Solecki
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Patent number: 7888011Abstract: The invention provides methods and related products for extracting nucleic acids such as DNA from prokaryotic spores. The invention also encompasses methods for identifying the source of such spores via analysis of the isolated nucleic acids.Type: GrantFiled: October 18, 2005Date of Patent: February 15, 2011Assignee: U.S. Genomics, Inc.Inventors: Trine Nilsen, Nuno Goncalves
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Patent number: 7879559Abstract: A method and kit for detecting Trichomonas vaginalis infection in a human subject are disclosed. In the method, a body-fluid sample such as a vaginal-swab sample or urine is obtained from the subject and contacted with an antibody specific against a Trichomonas adhesin peptide, forming an antibody-adhesin peptide complex if the subject is infected with Trichomonas. The presence or absence of the complex establishes, with a reliability of at least 80%, in the case of a vaginal swab sample, and with a reliability of at least 40% in the case of a urine sample, the presence or absence, respectively, of Trichomonas infection in the subject. A preferred test kit employs a dry-strip, sandwich assay, format.Type: GrantFiled: October 11, 2007Date of Patent: February 1, 2011Assignee: Xenotope Diagnostics, Inc.Inventors: John P. Alderete, Paul C. Castella
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Patent number: 7879330Abstract: Antibodies for binding epitopes of BoNT/A and hybridomas which produce such antibodies are described. The antibodies of the present invention can be used in a method for detecting BoNT/A in a sample and/or in a method for purifying BoNT/A from an impure solution. In addition, the antibodies can be used for passive immunization against BoNT/A intoxication or as intoxication therapy. Another aspect of the invention is a kit for detecting BoNT/A in a sample.Type: GrantFiled: March 6, 2006Date of Patent: February 1, 2011Assignee: The United States of America as represemted by the Secretary of the ArmyInventors: Sina Bavari, Edna R. Torres Melendez, Frank J. Lebeda
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Patent number: 7879576Abstract: The invention is the discovery of an actinomycete genus, given the name Salinospora gen. nov., that displays an obligate requirement of seawater (Na+) for growth and unique 16S rRNA signature nucleotides. The invention is also the use of the genus for the production and discovery of active biomolecules such as pharmaceutical agents, agrichemicals, immunomodifiers, enzymes and enzyme inhibitors.Type: GrantFiled: August 20, 2007Date of Patent: February 1, 2011Assignee: Regents of the University of CaliforniaInventors: William Fenical, Paul R. Jensen, Tracy J. Mincer
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Publication number: 20110020788Abstract: This invention relates generally to detection devices having one or more small wells each surrounded by, or in close proximity to, an NMR micro coil, each well containing a liquid sample with magnetic nanoparticles that self-assemble or disperse in the presence of a target analyte, thereby altering the measured NMR properties of the liquid sample. The device may be used, for example, as a portable unit for point of care diagnosis and/or field use, or the device may be implanted for continuous or intermittent monitoring of one or more biological species of interest in a patient.Type: ApplicationFiled: July 27, 2010Publication date: January 27, 2011Applicant: T2 Biosystems, Inc.Inventor: W. David Lee
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Publication number: 20110020240Abstract: Provided herein are imaging methods for detecting, diagnosing and imaging pathogenic bacteria or a pathophysiological condition associated therewith using fluorogenic substrates for bacterial enzymes. Fluorescent, luminescent or colorimetric signals emitted by substrates or enzyme products in the presence of the bacteria are compared to controls to detect and locate the pathogenic bacteria. Provided is a method for screening therapeutic agents to treat the pathophysiological conditions by measuring a signal emitted from the fluorogenic substrates or products in the presence and absence of the potential therapeutic agent. In addition, a diagnostic method for detecting a mycobacterial infection in a subject by contacting biological samples with a fluorogenic substrate and imaging for signals emitted from a mycobacterial beta-lactamase product.Type: ApplicationFiled: June 4, 2010Publication date: January 27, 2011Inventors: Jeffrey D. Cirillo, James C. Sacchettini, Jianghong Rao, Hexin Xie
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Publication number: 20110020845Abstract: The method for detecting and identifying a variant C. difficile strain in a sample is characterized by the following steps: (a) obtaining a sample of excreta or tissue from a human or animal patient body; (b) bringing the sample into contact with at least one antibody from each of at least three of the antibody groups group I, group II, group III, group IV, group V and group VI; (c) detecting the antibody reaction and constructing the reaction pattern; (d) comparing the reaction pattern obtained in (c) with reference patterns from known C. difficile strains and C. difficile strains the presence of which is to be tested in the investigation test; (e) assessing the agreement between the reaction pattern obtained in (c) and the reference pattern as indicative of the presence of the C. difficile strain of the reference pattern concerned in the sample.Type: ApplicationFiled: July 27, 2009Publication date: January 27, 2011Applicant: TGC BIOMICS GMBHInventors: Veit BRAUN, Karina GISCH, Michael Moos, Christoph von Eichel-Streiber
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Publication number: 20110020787Abstract: This invention relates generally to detection devices having one or more small wells each surrounded by, or in close proximity to, an NMR micro coil, each well containing a liquid sample with magnetic nanoparticles that self-assemble or disperse in the presence of a target analyte, thereby altering the measured NMR properties of the liquid sample. The device may be used, for example, as a portable unit for point of care diagnosis and/or field use, or the device may be implanted for continuous or intermittent monitoring of one or more biological species of interest in a patient.Type: ApplicationFiled: July 27, 2010Publication date: January 27, 2011Applicant: T2 Biosystems, Inc.Inventor: W. David Lee
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Publication number: 20110021374Abstract: This invention relates generally to detection devices having one or more small wells each surrounded by, or in close proximity to, an NMR micro coil, each well containing a liquid sample with magnetic nanoparticles that self-assemble or disperse in the presence of a target analyte, thereby altering the measured NMR properties of the liquid sample. The device may be used, for example, as a portable unit for point of care diagnosis and/or field use, or the device may be implanted for continuous or intermittent monitoring of one or more biological species of interest in a patient.Type: ApplicationFiled: July 27, 2010Publication date: January 27, 2011Applicant: T2 Biosystems, Inc.Inventor: W. David Lee
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Patent number: 7875435Abstract: A method and apparatus for use in a flow through assay process is disclosed. The method is characterized by a “pre-incubation step” in which the sample which is to be analysed (typically for the presence of a particular protein), and a detection analyte (typically one or more antibodies bound to colloidal gold or a fluorescent tag) which is known to bind to the particular protein may bind together for a desired period of time. This pre-incubation step occurs before the mixture of sample and detection analyte come into contact with a capture analyte bound to a membrane. The provision of the pre-incubation step has the effect of both improving the sensitivity of the assay and reducing the volume of sample required for an assay. An apparatus for carrying out the method is disclosed defining a pre-incubation chamber for receiving the sample and detection analyte having a base defined by a membrane and a second membrane to which a capture analyte is bound.Type: GrantFiled: December 12, 2002Date of Patent: January 25, 2011Assignee: Proteome Systems LtdInventors: David Ian Marlborough, Andrew John Sloane, Robert Alan Cole, William Samuel Hunter
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Patent number: 7875421Abstract: The present invention pertains to the use of the DNA sequence of a Lactobacillus johnsonii strain, in particular to its genomic sequence for elucidating interactions of micro-organism with hosts they colonize, and moreover for elucidating the basis of probiotic properties exhibited by such strain. In addition, the present invention also relates to methods of detecting nucleic acids or polypeptides of Lactobacilli and related species, respectively. A data carrier is provided comprising nucleotide sequences and/or polypeptide sequences of La1.Type: GrantFiled: October 2, 2008Date of Patent: January 25, 2011Assignee: Nestec S.A.Inventors: Raymond David Pridmore, Beat Mollet, Fabrizio Arigoni, Josef Hermanns
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Publication number: 20110014599Abstract: The present invention provides fluorescent dyes that are based on firefly luciferin structure. These dyes are optimally excited at shorter wavelengths and have Stokes shift of at least 50 nm. The fluorescent dyes of the invention are useful for preparation of dye-conjugates, which can be used in detection of an analyte in a sample.Type: ApplicationFiled: July 15, 2010Publication date: January 20, 2011Applicant: BECKMAN COULTER, INC.Inventors: Hashem AKHAVAN-TAFTI, Renuka DE SlLVA, Guoping WANG, Robert A. EICKHOLT, Ravinder K. GUPTA, Lakshmi S. KAANUMALLE
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Publication number: 20110008801Abstract: The invention provides an atomic force microscope-based bioassay, assisted by thermodynamic characterizations to quickly and accurately screen for compounds that disrupt cell-cell or cell-substrate interactions.Type: ApplicationFiled: March 20, 2007Publication date: January 13, 2011Applicant: Worcester Polytechnic InstituteInventors: Terri A. Camesano, Yatao Liu, Amparo Gallardo-Moreno
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Publication number: 20110003306Abstract: Subject of the present invention is a method for detection of an antibiotic resistance in a micro-organism comprising the steps of exposing suspected micro-organism to a labelled (fluorescent) antibiotic and observing the differences between it and a non-resistant micro-organism of the same type.Type: ApplicationFiled: January 30, 2009Publication date: January 6, 2011Applicant: MIACOM DIAGNOSTICS GMBHInventors: Ian Thrippleton, Walter Stein