Streptococcus Patents (Class 435/7.34)
  • Patent number: 11260390
    Abstract: A lateral-flow assay device includes a substrate having a sample addition zone and a wash addition zone downstream thereof along a fluid flow path through which a sample flows. The fluid flow path is configured to receive a wash fluid in the wash addition zone. A hydrophilic surface is arranged in the wash addition zone. Flow constriction(s) are spaced apart from the fluid flow path and arranged to define, with the hydrophilic surface, a reservoir configured to retain the wash fluid by formation of a meniscus between the hydrophilic surface and the flow constriction(s). The fluid flow path draws the wash fluid from the reservoir by capillary pressure. Apparatus for analyzing a fluidic sample and methods of displacing a fluidic sample in a fluid flow path of an assay device are also described.
    Type: Grant
    Filed: August 3, 2018
    Date of Patent: March 1, 2022
    Assignee: ORTHO-CLINICAL DIAGNOSTICS, INC.
    Inventors: Zhong Ding, Edward R. Scalice, Daniel P. Salotto
  • Patent number: 9886750
    Abstract: An electronic device for reading diagnostic test results and collecting subject data for inclusion in a local chain of evidence database and for transferring and receiving data from remote databases.
    Type: Grant
    Filed: May 8, 2015
    Date of Patent: February 6, 2018
    Inventors: William Pat Price, Ted Titmus
  • Publication number: 20150132740
    Abstract: A concentration agent for microorganisms is provided that contains both lanthanum and carbonate. Additionally, articles that include the concentration agent and methods of concentrating a microorganism using the concentration agent are provided.
    Type: Application
    Filed: May 23, 2013
    Publication date: May 14, 2015
    Inventors: Manjiri T. Kshirsagar, Evan Koon Lun Yuuji Hajime, Andrew W. Rabins
  • Publication number: 20150125884
    Abstract: The present invention provides novel methods of magnetically labeling a bacterial cell by contacting the call with an affinity ligand and subsequently contacting the cell with a magnetic agent, where the affinity ligand and magnetic agent include bioorthogonally reactive groups that can react with each other to form a covalent bond. Compounds, compositions, kits and applications of the method are also described.
    Type: Application
    Filed: March 8, 2013
    Publication date: May 7, 2015
    Inventors: Ghyslain Budin, Ralph Weissleder, Hakho Lee, Hyun Jung Chung
  • Publication number: 20150072880
    Abstract: A device for detecting at least one analyte generally comprises (i) a support having a chamber for receiving a biological fluid therein, wherein said chamber is an elongate chamber having a length axis; (ii) a carrier or agitator in said elongate chamber, said carrier or agitator having opposite end portions and a side portion, the carrier or agitator dimensioned to travel in said chamber along said length axis and/or permit said liquid sample to flow in the chamber therearound, either (or both) thereby agitating the liquid sample; and (iii) at least one anti-analyte antibody coupled to either the carrier and/or the chamber side wall. Methods of using the device are also described.
    Type: Application
    Filed: May 9, 2013
    Publication date: March 12, 2015
    Inventors: David A. Calderwood, Rodolfo R. Rodriguez
  • Patent number: 8921060
    Abstract: The invention relates to the area of health-beneficial preparations and production methods thereof, in particular to the use of thermally pre-treated Lactobacillus preparations having specific bonding capacity for Streptococcus mutans for caries prophylaxis. The invention further relates to the Lactobacillus preparations and thermal pasteurization.
    Type: Grant
    Filed: October 11, 2010
    Date of Patent: December 30, 2014
    Assignee: BASF SE
    Inventor: Bryan Cooper
  • Patent number: 8906630
    Abstract: The present invention encompasses methods, assays and kits for the detection of pathogenic bacteria in a patient and for the differential diagnosis of pulmonary diseases associated with pathogenic bacteria from pulmonary diseases not associated with pathogenic bacteria.
    Type: Grant
    Filed: February 25, 2005
    Date of Patent: December 9, 2014
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: Jeffrey N. Weiser, Jane M. Gould
  • Publication number: 20140335544
    Abstract: Methods for detecting in a sample the presence of an antibody to a conjugate of an antigen associated with a first carrier by a first association are disclosed. The method comprises contacting a conjugate of the antigen associated with a second carrier by a second association with said sample under conditions that allow binding of the antibody to the antigen; and introducing an agent to detect the presence of the antibody bound to said antigen. The first association and the second association are covalent associations and the first association is different from the second association. Also provided are kits, multiwell plates and conjugates that are useful in the method and further uses of the method. Also provided is a method of releasing a batch of a vaccine comprising a conjugate of an antigen associated with a first carrier by a first association and antibodies useful in this method.
    Type: Application
    Filed: December 12, 2012
    Publication date: November 13, 2014
    Inventors: Francesco Berti, Carl E. Frasch
  • Publication number: 20140323340
    Abstract: The present invention relates to moving microorganisms to a surface, where they are grown in the presence and absence of antimicrobials, and by monitoring the growth of the microorganisms over time in the two conditions, their susceptibility to the antimicrobials can be determined. The microorganisms can be moved to the surface through electrophoresis, centrifugation or filtration. When the movement involves electrophoresis, the presence of oxidizing and reducing reagents lowers the voltage at which electrophoretic force can be generated and allows a broader range of means by which the target can be detected. Monitoring can comprise optical detection, and most conveniently includes the detection of individual microorganisms. The microorganisms can be stained in order to give information about their response to antimicrobials.
    Type: Application
    Filed: July 11, 2014
    Publication date: October 30, 2014
    Inventors: David A. Goldberg, David C. Howson, Steven W. Metzger, Daniel A. Buttry, Steven Scott Saavedra
  • Publication number: 20140315221
    Abstract: The present disclosure relates to devices and methods for rapidly detecting streptococcus bacteria. An illustrative device includes a first elongate handle connected to a first edible portion and a second elongate handle connected to a second edible portion. The first and second elongate handles are connected by a banding material. The first and second edible portions are inserted into the mouth of an individual for receiving a saliva sample.
    Type: Application
    Filed: March 17, 2014
    Publication date: October 23, 2014
    Inventor: Kathleen Morsey
  • Publication number: 20140315219
    Abstract: The present invention provides a rapid, highly sensitive and specific enzyme-linked immunosorbent assay (ELISA), referred to as N-Assay, a device, and a kit, for detection and identification of microorganisms in a sample, in thirty minutes or less, with little or no interference from non-target microorganisms. The present invention also provides for simultaneous determination of antimicrobial susceptibility of microorganism in the N-Assay.
    Type: Application
    Filed: April 18, 2014
    Publication date: October 23, 2014
    Inventors: Bret T. Barnhizer, Jonathan P. Faro
  • Publication number: 20140294930
    Abstract: In alternative embodiments, the invention provides vaccines, pharmaceutical compounds and formulations for diagnosing, preventing, treating or ameliorating Group A Streptococcus (GAS), Group C Streptococcus (GCS), or Group A Streptococcus (GGS), infections, or other pathogenic Streptococcus infections. In alternative embodiments, the invention provides compositions such as diagnostic tests, assays, immunoassays and test strips, and methods, for detecting or diagnosing the presence of a Streptococcal infection, e.g., Group A Streptococcus (GAS), Group C Streptococcus (GCS), or Group A Streptococcus (GGS), infections, or other pathogenic Streptococcus infections.
    Type: Application
    Filed: August 3, 2012
    Publication date: October 2, 2014
    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Victor Nizet, Nina van Sorge
  • Patent number: 8785148
    Abstract: Described herein is a method and a device for expediting delivery of an agent to a damaged bacterial cell. In one embodiment, the methods and devices are useful for screening candidate antibiotics. In another embodiment, the methods and devices described herein are used to determine susceptibility of bacteria to an antibiotic. The methods also provide a method for determining an appropriate antibiotic to treat an individual having a bacterial infection.
    Type: Grant
    Filed: October 28, 2011
    Date of Patent: July 22, 2014
    Assignees: Fraunhofer, USA, Inc., Trustees of Boston University
    Inventors: Alexis Sauer-Budge, Andre Sharon, Maxim Kalashnikov, Holger Wirz
  • Publication number: 20140170639
    Abstract: The invention generally relates to methods for indicating whether an assay for isolating targets is properly isolating and detecting targets. Methods of the invention involve obtaining a sample suspected of a containing target, introducing a detectable marker into the sample, conducting an assay using magnet particles to isolate the detectable marker and the target if it is present in the sample, determining the presence or absence of the target; and confirming that the assay functioned properly by determining presence or absence of the detectable marker.
    Type: Application
    Filed: December 12, 2013
    Publication date: June 19, 2014
    Applicant: NANOMR, INC.
    Inventor: Meghan Norvell
  • Publication number: 20130315958
    Abstract: Disclosed is a new and emerging serotype of Streptococcus pneumoniae designated serotype 6C, and assays and monoclonal antibodies useful in identifying same. Also disclosed is a novel pneumococcal polysaccharide with the repeating unit {2) glucose 1 (1?3) glucose 2 (1?3) rhamnose (1?3) ribitol (5?phosphate}. This new serotype may be included in pneumococcal vaccines.
    Type: Application
    Filed: May 9, 2013
    Publication date: November 28, 2013
    Inventors: Moon H. Nahm, Jisheng Lin, Angela P. Brandao, Maria Cristina Brandileone
  • Publication number: 20130230462
    Abstract: Disclosed are methods of identifying an individual having a predisposition to the development of a cardiovascular disease, and administering a suitable prophylactic agent to the identified individual to prevent disease. Methods of screening and identifying agents that inhibit bacterial collagen binding protein (CBP)-mediated cell invasion are also disclosed.
    Type: Application
    Filed: August 22, 2011
    Publication date: September 5, 2013
    Applicants: UNIVERSITY OF FLORIDA, UNIVERSITY OF ROCHESTER
    Inventors: Jacqueline Abranches, José A. Lemos, Robert A. Burne
  • Patent number: 8507203
    Abstract: The invention is directed to aptamers, methods and kits comprising same for detecting Streptococcus pyogenes. The method of screening a subject for Streptococcus pyogenes involves obtaining a body sample from the subject; contacting the sample or a bacterial culture of the sample with an aptamer or a panel of aptamers specific to S. pyogenes; and detecting the presence or absence of S. pyogenes in the sample or the bacterial culture, wherein binding of the aptamer is indicative of the presence of S. pyogenes.
    Type: Grant
    Filed: March 29, 2012
    Date of Patent: August 13, 2013
    Assignee: The Governors of the University of Alberta
    Inventors: X. Chris Le, Camille Hamula, Xing-Fang Li
  • Publication number: 20130129737
    Abstract: This invention provides an isolated nucleic acid encoding a polypeptide comprising amino acid sequences of a streptococcal matrix adhesion E (EmaE) polypeptide. Antibodies to the EmaE polypeptide and immunogenic fragments thereof are also provided. This invention provides pharmaceutical compositions, immunogenic compositions, vaccines, and diagnostic and therapeutic methods of use of the isolated polypeptide, antibodies thereto, and nucleic acids.
    Type: Application
    Filed: February 18, 2011
    Publication date: May 23, 2013
    Applicants: University of Utah Research Foundation, St. Jude Children's Research Hospital
    Inventors: Elisabeth Adderson, John Bohnsack
  • Patent number: 8445293
    Abstract: The present invention includes methods and devices for preventing interfering substances from affecting the accuracy of a lateral flow immunoassay. In preferred embodiments, a test strip includes a capturing zone that includes at least one mobile capturing reagent that separates at least one interfering substance from the analyte. The capturing zone is preferably located upstream of the sample application zone. In some embodiments, the reagent/conjugate zone is also located upstream of the sample application zone. The capturing zone may be located upstream, downstream, or overlapping with the reagent/conjugate zone in these embodiments. In other preferred embodiments, one or more mobile capturing reagents are included in the elution medium/running buffer. In yet other embodiments, the capturing reagent is incorporated into a sample collection device of a sample collection system, preferably separate from the chromatographic test strip. A lysis zone is also included in some preferred embodiments.
    Type: Grant
    Filed: September 29, 2009
    Date of Patent: May 21, 2013
    Assignee: Rapid Pathogen Screening, Inc.
    Inventors: Uma Mahesh Babu, Franz Aberl, Marcus Scheibenzuber, Robert P. Sambursky, Robert W. VanDine, Jose S. Sambursky
  • Publication number: 20130078259
    Abstract: A method of providing protection against pneumococcal infection in a subject is disclosed. The method includes steps of administering to the subject a composition that includes combination of three recombinant pneumococcal neuraminidases: NanA, NanB, and NanC of S. pneumoniae strains CGSP14, wherein administration of the recombinant pneumococcal neuraminidases elicits an immune response to S. pneumoniae, and treats the subject. In one embodiment, the method further includes a step of adding adjuvants to enhance the immune response. The method also includes a step of using passive antibodies, wherein said passive antibodies are anti-neuraminidase antibodies generated from neuraminidases-immunized humanized animals: NanA, NanB, and NanC. Meanwhile, this invention also provides a method for the molecular diagnosis of pneumococcal infection.
    Type: Application
    Filed: September 25, 2011
    Publication date: March 28, 2013
    Applicant: CHANG GUNG MEDICAL FOUNDATION, LINKOU BRANCH
    Inventors: Cheng-Hsun Chiu, Rajendra Prasad Janapatla, Chyi-Liang Chen, Mei-Hua Hsu, Hsiu-Ling Chen, Chung-Tsui Huang, Hsin-Ju Chang, Wan-Ting Liao
  • Patent number: 8404457
    Abstract: Assays for detecting anti-streptococcal antibodies in biological samples using one or more streptococcal antigens are described herein. Various combinations of antigens may be used in the assays. For example, one or more of Ply, PhtD, PhtE, LytB and PcpA may be utilized. Additional streptococcal antigens may also be used. The assays may also be used in combination with assays that detect streptococcal nucleic acids.
    Type: Grant
    Filed: February 2, 2009
    Date of Patent: March 26, 2013
    Assignee: Sanofi Pasteur Limited
    Inventors: Martina Ochs-Onolemhemhen, Roger Brookes, Claire-Anne Siegrist
  • Publication number: 20130011862
    Abstract: The present invention relates to processes for the rapid detection, semi-quantification and quantification of live microorganisms in solutions or suspensions using immunomagnetic particles, without requiring pre-enrichment through culture of the microorganism. The invention also relates to kits for carrying out said processes and to the quantification of the microorganisms detected by means of automated biosensor equipment.
    Type: Application
    Filed: November 14, 2011
    Publication date: January 10, 2013
    Applicant: BIOTICA, BIOQUIMICA ANALITICA, S.L.
    Inventors: Guillermo RODRIGUEZ ALBALAT, Maria Luisa JIMENEZ BONO, Daniel CANOS MARTI
  • Publication number: 20130011428
    Abstract: The present invention relates to newly identified open reading frames comprised within the genomic nucleotide sequence of Streptococcus pneumoniae, wherein the open reading frames encode polypeptides that are surface localized on Streptococcus pneumoniae. Thus, the invention relates to Streptococcus pneumoniae open reading frames that encode polypeptides encoded by the Streptococcus pneumoniae open reading frames, vectors comprising open reading frame sequences and cells or animals transformed with these vectors. The invention relates also to methods of detecting these nucleic acids or polypeptides and kits for diagnosing Streptococcus pneumoniae infection. The invention finally relates to pharmaceutical compositions, in particular immunogenic compositions, for the prevention and/or treatment of bacterial infection, in particular infections with Streptococcus pneumoniae.
    Type: Application
    Filed: July 11, 2012
    Publication date: January 10, 2013
    Applicant: WYETH HOLDINGS CORPORATION
    Inventors: Robert John Zagursky, Amy Wadhams Masi, Bruce Arthur Green, Deb Narayan Chakravarti, David Parrish Russell, Joseph Lawrence Wooters
  • Publication number: 20120315623
    Abstract: The disclosure relates to the detection of analytes (e.g., biological pathogens such as bacteria or viruses) using a conductive polymer label. The disclosed detection system utilizing the conductive polymer label generally involves the formation of an analyte conjugate between the target analyte and a conductive polymer moiety conjugated to the target analyte. The conductive polymer portion of the analyte conjugate is electrically activated to form an electrically activated analyte conjugate having an increased electrical conductivity relative to the analyte conjugate as originally formed. The electrically activated analyte conjugate can then be detected by any suitable means, such as by conductimetric or electrochemical detection.
    Type: Application
    Filed: May 22, 2012
    Publication date: December 13, 2012
    Applicant: BOARD OF TRUSTEES OF MICHIGAN STATE UNIVERSITY
    Inventors: Evangelyn C. Alocilja, Emma Setterington
  • Publication number: 20120252005
    Abstract: The present invention discloses methods for detecting the presence of a complex between a first reagent and a second reagent in solution. In particular, the invention provides methods for qualitative or quantitative detection of an analyte or its specific binding partner in complex biological samples. The invention further discloses algorithms using summary rate changes at selected wavelengths in the absorbance spectra of colloidal metal-labeled analytes or specific binding partners to identify intermolecular interactions between the analyte and its binding partner.
    Type: Application
    Filed: March 19, 2012
    Publication date: October 4, 2012
    Applicant: Abaxis, Inc.
    Inventors: Vincent CHIANG, Rajesh K. Mehra
  • Patent number: 8263356
    Abstract: The present invention relates to antibodies, binding portions thereof, or probes that bind specifically to glucosyltransferase enzymes, and uses of these agents for detecting glucosyltransferase enzyme(s) in a sample and for diagnosing predisposition of a human child to early childhood caries. The present invention also relates to a kit for detecting a glucosyltransferase enzyme in an oral sample from an animal.
    Type: Grant
    Filed: August 14, 2006
    Date of Patent: September 11, 2012
    Assignee: University of Rochester
    Inventors: William H. Bowen, Anne Vacca Smith, Robert Berkowitz
  • Publication number: 20120225423
    Abstract: The invention described herein provides methods for the detection of target particles, such as pathogens, soluble antigens, nucleic acids, toxins, chemicals, plant pathogens, blood borne pathogens, bacteria, viruses and the like. Also described is an emittor cell comprising a receptor, wherein the receptor can be an antibody or an Fc receptor, and an emittor molecule for the detection of a target particle in a sample wherein the target particle to be detected is bound by one or more receptors on the emittor cell. Also provided are optoelectronic sensor devices for detecting a target particle in a sample, including in a plurality of samples.
    Type: Application
    Filed: April 24, 2012
    Publication date: September 6, 2012
    Applicant: Massachusetts Institute of Technology
    Inventors: Eric Schwoebel, James Harper, Martha S. Petrovick, Frances Nargi, Mark Hollis, Bernadette Johnson, Joseph Lacirignola, Richard Mathews, Kristine Hogan, Trina Vian, Allan Heff, Mark Hennessy, Songeeta Palchaudhuri, Todd Rider
  • Patent number: 8252546
    Abstract: Compositions and methods for eliciting an immune response against Streptococcus pneumoniae are described. More particularly, the present disclosure relates to immunogenic PcpA polypeptides, including fragments of PcpA and variants thereof, and nucleic acids that encode the polypeptides. The present disclosure further relates to methods of making and using the immunogenic polypeptides. Further provided is a method of diagnosing pneumococcal infection (e.g., pneumonia) in a subject by obtaining a biological sample from the subject and detecting one or more pneumococcal antigens that are selectively expressed during invasion (e.g., PcpA or fragments thereof).
    Type: Grant
    Filed: August 17, 2007
    Date of Patent: August 28, 2012
    Assignee: The UAB Research Foundation
    Inventors: David E. Briles, Susan K. Hollingshead
  • Patent number: 8192926
    Abstract: The present invention is directed to methods for extracting markers from biological samples, and to systems, devices, kits and reagents for use in such methods. The invention is also to methods, kits, reagents and compositions for measuring a plurality of different organism types in a sample. One of the specific advantages of the present invention is the ability to simultaneously extract more than one microorganism or viral particle marker in one volume from a single sample containing a complex biological matrix.
    Type: Grant
    Filed: May 19, 2006
    Date of Patent: June 5, 2012
    Assignee: Meso Scale Technologies LLC
    Inventors: Jeff D. Debad, Cindy V. Ly
  • Publication number: 20120129157
    Abstract: The invention provides methods, devices and kits for rapid detection and identification of one or more live target microorganisms in a liquid sample or grown on plates containing nutrient media. The invention includes mixing one or more target microorganisms with one or more aptamers and/or one or more antibodies, each conjugated to a reporter compound and specific for a first site on one or more target microorganisms to form a mixture. The mixture is placed on a permeable membrane having immobilized thereon one or more aptamers linked to an amine compound, and/or one or more antibodies, each specific for a second site on one or more target microorganisms or a site on the aptamer conjugate and/or antibody conjugate. A detection solution is added to the membrane and detection and identification of one or more target microorganisms is achieved in about one hour or less.
    Type: Application
    Filed: January 13, 2012
    Publication date: May 24, 2012
    Inventors: Bret T. Barnhizer, Sergey Gazenko
  • Patent number: 8163502
    Abstract: Provided are methods, test devices, and diagnostic kits for predicting, assessing, and diagnosing the risk of a disease using salivary analysis. The methods comprise providing a whole (unfractionated) saliva sample from a subject; contacting an aliquot of the saliva with two or more lectins under conditions that allow the two or more lectins to bind to a lectin-binding component of the saliva; detecting the amount of bound lectin; and comparing the amount of bound lectin to the amount known to bind a saliva sample from a control patient, to predict the risk of a disease in the subject. Also provided are methods for reducing the risk of a disease and a method for assessing the risk of the disease at a defined level.
    Type: Grant
    Filed: May 24, 2010
    Date of Patent: April 24, 2012
    Assignees: Proactive Oral Solutions, Inc., University of Southern California
    Inventors: Patricia Denny, Paul C. Denny, Mahvash Navazesh
  • Publication number: 20120034617
    Abstract: The invention herein generally relates to kits and methods for detecting the presence of a bacterium in a subject, for example, methicillin resistant S. aureus.
    Type: Application
    Filed: March 31, 2010
    Publication date: February 9, 2012
    Inventors: Ambrose Lin-Yau Cheung, George Lee Newcomb
  • Publication number: 20120003661
    Abstract: Methods and devices are provided for the rapid and specific detection of target microorganisms, cells, and the like. In one embodiment, the methods involve contacting a target microorganism (e.g., in a sample) with a permeabilization reagent that selectively permeabilizes or lyses the microorganism; contacting the selectively permeabilized microorganism with a detection reagent that is taken into the selectively permeabilized organism or that contacts metabolites or enzymes released by the selectively permeabilized microorganism, where the detection reagent produces a signal in the presence of said metabolites or enzymes; and detecting a signal produced by the detection reagent in the presence of the metabolites or enzymes wherein the strength of the signal indicates the presence and/or amount of the target microorganism in the sample.
    Type: Application
    Filed: June 27, 2011
    Publication date: January 5, 2012
    Applicant: C3 JIAN, INC.
    Inventors: Randal H. Eckert, Chris Kaplan, Jian He, Daniel K. Yarbrough, Maxwell Anderson, Jee-Hyun Sim
  • Publication number: 20110171252
    Abstract: Described are Streptococcus suis infections in pigs, vaccines directed against those infections and tests for diagnosing S. suis infections. Provided is an isolated or recombinant nucleic acid encoding a capsular gene cluster of S. suis or a gene or gene fragment derived therefrom. Further provided is a nucleic acid probe or primer allowing species or serotype-specific detection of S. suis. Also provided is a Streptococcus suis antigen and vaccine derived therefrom.
    Type: Application
    Filed: June 16, 2010
    Publication date: July 14, 2011
    Inventor: Hilda Elizabeth Smith
  • Patent number: 7932051
    Abstract: The present invention provides a test medium comprising a test microorganism, an indicator and a metal in ionized form, wherein the valence of said metal is at least 2 and the concentration of said metal is between 0.001 M and 1 M. Furthermore, there is provided a method for the determination of the presence of an antibiotic characterized in that a metal salt is added to said test medium and/or to said sample of fluid. Finally, there is provided a kit suitable for the determination of an antibiotic in a fluid.
    Type: Grant
    Filed: September 6, 2004
    Date of Patent: April 26, 2011
    Assignee: R-Biopharm AG
    Inventors: Edith Magda Lucia Geijp, Jacobus J. Stark
  • Publication number: 20110086369
    Abstract: The present invention describes novel fluorescent compounds analogous to alkyl phospholipids, containing highly photostable fluorescent groups in the structure thereof and emitting in the visible spectrum wavelength zone. Said compounds are easily introduced to microorganisms, enabling the detection and identification of these organisms by means of fluorescence microscopy. The novel fluorescent compounds can also diagnose, rapidly and easily, the presence of pathogenic microorganisms characterised in that they are resistant to treatment with alkyl phospholipids.
    Type: Application
    Filed: March 30, 2009
    Publication date: April 14, 2011
    Inventors: A. Ulises Acuña Fernández, Francisco Amat Guerri, Eugenia Carrillo Gallego, Valentín Hornillos Gomez Recuero, Susana Marcos Celestino, José María Requejo Isidro, Luis Ignacio Rivas López, José María Saugar Cruz, Carmen Del Aguila De La Puente, Jesús Merayo Lloves
  • Publication number: 20110020240
    Abstract: Provided herein are imaging methods for detecting, diagnosing and imaging pathogenic bacteria or a pathophysiological condition associated therewith using fluorogenic substrates for bacterial enzymes. Fluorescent, luminescent or colorimetric signals emitted by substrates or enzyme products in the presence of the bacteria are compared to controls to detect and locate the pathogenic bacteria. Provided is a method for screening therapeutic agents to treat the pathophysiological conditions by measuring a signal emitted from the fluorogenic substrates or products in the presence and absence of the potential therapeutic agent. In addition, a diagnostic method for detecting a mycobacterial infection in a subject by contacting biological samples with a fluorogenic substrate and imaging for signals emitted from a mycobacterial beta-lactamase product.
    Type: Application
    Filed: June 4, 2010
    Publication date: January 27, 2011
    Inventors: Jeffrey D. Cirillo, James C. Sacchettini, Jianghong Rao, Hexin Xie
  • Publication number: 20100278740
    Abstract: Protein antigens from Streptococcus pneumoniae are disclosed, together with nucleic acid sequences encoding them. Their use in vaccines and in screening methods is also described.
    Type: Application
    Filed: March 26, 2009
    Publication date: November 4, 2010
    Applicant: Sanofi Pasteur Limited
    Inventors: Christophe François Guy GILBERT, Philip Michael Hansbro
  • Publication number: 20100216711
    Abstract: The present invention relates to a modified polypeptide with a biological activity to lyse cell walls of bacteria, wherein the polypeptide has no caspase, clostripain, enterokinase, factor Xa, granzyme B, staphylococcus peptidase I (V8 Protease), plasmin, streptopain, bacillolysin and/or thrombin cleavage site. The invention further relates to nucleic acids with a sequence encoding a polypeptide according to the present invention.
    Type: Application
    Filed: August 19, 2008
    Publication date: August 26, 2010
    Inventors: Holger Grallert, Michael Forchheim
  • Publication number: 20100159441
    Abstract: The present invention discloses methods for detecting the presence of a complex between a first reagent and a second reagent in solution. In particular, the invention provides methods for qualitative or quantitative detection of an analyte or its specific binding partner in complex biological samples. The invention further discloses algorithms using summary rate changes at selected wavelengths in the absorbance spectra of colloidal metal-labeled analytes or specific binding partners to identify intermolecular interactions between the analyte and its binding partner.
    Type: Application
    Filed: March 18, 2009
    Publication date: June 24, 2010
    Applicant: ABAXIS, INC.
    Inventors: VINCENT CHIANG, RAJESH K. MEHRA
  • Patent number: 7736857
    Abstract: Provided are methods, test devices, and diagnostic kits for predicting, assessing, and diagnosing the risk of a disease using salivary analysis. The methods comprise providing a whole (unfractionated) saliva sample from a subject; contacting an aliquot of the saliva with two or more lectins under conditions that allow the two or more lectins to bind to a lectin-binding component of the saliva; detecting the amount of bound lectin; and comparing the amount of bound lectin to the amount known to bind a saliva sample from a control patient, to predict the risk of a disease in the subject. Also provided are methods for reducing the risk of a disease and a method for assessing the risk of the disease at a defined level.
    Type: Grant
    Filed: April 1, 2004
    Date of Patent: June 15, 2010
    Assignees: Proactive Oral Solutions, Inc., University of Southern California
    Inventors: Patricia Denny, Paul C. Denny, Mahvash Navazesh
  • Publication number: 20100136057
    Abstract: The invention relates to the field of diagnosis of and vaccination against Streptococcal infections and to the detection of virulence markers of Streptococci. The invention discloses a method for modulating virulence of a Streptococcus, the method comprising modifying a genomic fragment of Streptococcus wherein the genomic fragment comprises at least a functional part of a fragment identifiable by hybridization in Streptococcus suis to a nucleic acid or fragment thereof as shown in FIG. 5.
    Type: Application
    Filed: December 3, 2009
    Publication date: June 3, 2010
    Inventor: Hilda Elizabeth Smith
  • Patent number: 7718375
    Abstract: The present invention relates to modifying rapid immunochromatographic (“ICT”) tests for the detection of characteristic carbohydrate antigens of bacteria that are known to be causative of otitis media and respiratory diseases in children under the age of approximately 12 years. The test modifications involve either (1) reducing the total amount of antibodies to the carbohydrate antigen employed in each test, (2) adding at least one fixed “scrub” line located just prior to the capture line in the sample flow path to the prepared ICT test strip to “scrub” out an identical amount of target antigen from all bodily fluid test samples obtained from both colonized but otherwise healthy children and diseased children, or (3) combinations of (1) and (2).
    Type: Grant
    Filed: February 27, 2002
    Date of Patent: May 18, 2010
    Assignee: Binax, Inc.
    Inventors: Roger N. Piasio, Madeline Wareing
  • Publication number: 20100119507
    Abstract: The present invention relates to antigens, more particularly antigenS of Streptococcus pyogenes (also called group A Streptococcus (GAS)) bacterial pathogen which are useful as vaccine component for therapy and/or prophylaxis.
    Type: Application
    Filed: September 28, 2009
    Publication date: May 13, 2010
    Applicant: ID BIOMEDICAL CORPORATION
    Inventors: Denis Martin, Bernard Brodeur, Josee Hamel, Stephane Rioux, Patrick Rheault
  • Patent number: 7709210
    Abstract: The present invention relates to a method for assessing the likelihood of the presence or formation of fetal heart disease (such as HLHS) in a fetus. In this methodology, the mother, either before or during pregnancy, is tested for the presence of anti-strep antibodies. If positive, the fetus is evaluated and monitored for the presence of fetal heart disease; the fetus can be treated, if appropriate. In addition, either prior to pregnancy or during the first trimester, the mother can be treated to prevent the formation of such antibodies or to neutralize their presence or effect fetal heart tissue.
    Type: Grant
    Filed: October 4, 2006
    Date of Patent: May 4, 2010
    Assignee: Children's Hospital Medical Center
    Inventor: Pirooz Eghtesady
  • Publication number: 20100055721
    Abstract: Multiplexed lateral flow assays, related methods, and devices are disclosed which are capable of simultaneously detecting multiple analytes. The assays are preferably immunoassays and can be multiplexed spatially, spectrally, and both spatially and spectrally. Multiplexed assays are disclosed employing quantum dots for applications including the detection of human proteins and the monitoring of microorganisms relevant to water contamination. The multiplexed assays can employ one or more species of Surface Enhanced Raman Scattering nanoparticles, with one or more species having a unique Raman shift spectrum. The invention is widely adaptable to a variety of analytes such as biowarfare agents, human clinical markers, and other substances.
    Type: Application
    Filed: April 3, 2009
    Publication date: March 4, 2010
    Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: James L. LAMBERT, Anita M. Fisher
  • Publication number: 20100047222
    Abstract: The full-length nucleic acid sequence of the C1 bacteriophage is disclosed in the present application The specific regions of the C1 genome encoding the PlyC lysin have also been identified and sequenced. The invention relates to the pharmaceutical and diagnostic utility of these sequences and provides for development of pharmaceutical compositions for treating or preventing streptococcal infections in mammals, for compositions for decontamination of inanimate surfaces and for diagnosis of streptococcal infections.
    Type: Application
    Filed: July 23, 2009
    Publication date: February 25, 2010
    Inventors: Vincent Fischetti, Daniel Nelson, Raymond Schuch
  • Publication number: 20100035234
    Abstract: The present invention is directed to methods, assays and compositions for implementing such methods and assays for assessing efficacy of individual components in multi-component vaccines and for assessing efficacy of a vaccine against a pathogen. In one aspect, the method of assessing efficacy of a vaccine against a pathogen is a quick assay that tests for an activity correlated with efficacy such as binding in an ELISA rather than requiring the time and expense of an assay that detects actual bactericidal activity. In another aspect, the method for testing the efficacy of an individual component in a multi-component vaccine includes obtaining an immune sample from a subject inoculated with the multi-component vaccine; blocking the portion of the immune sample that recognizes the individual component such as by addition of the individual component, and testing the efficacy of the immune sample to respond to the pathogen.
    Type: Application
    Filed: May 19, 2009
    Publication date: February 11, 2010
    Applicant: Novartis AG
    Inventors: John Donnelly, Ping Wu, George Santos, Marzia Monica Giuliani, William Andrews, Jie Chen
  • Patent number: 7642068
    Abstract: This application discloses a multivalent opsonophagocytosis assay that does not rely on counting of bacterial colonies to determine bacteria viability following opsonophagocytosis. Instead, the method uses a metabolic colorimetric indicator to determine if viable bacteria are present. Also disclosed are arrays that can be used to determine the viability of bacteria following opsonophagocytosis.
    Type: Grant
    Filed: April 21, 2006
    Date of Patent: January 5, 2010
    Assignee: The United States of America as represented by the Department of Health and Human Services, Center for Disease Control and Prevention
    Inventors: Sandra Steiner, Patricia F. Holder, George M. Carlone, GowriSankar Rajam
  • Publication number: 20090269789
    Abstract: An antibody for detection of microorganisms, a method of detection of microorganisms, and a reagent kit for detection of microorganisms, which is species specific for every species of microorganisms and with which all serototypes within the same species can be detected, are provided. Antibody to intracellular molecules with the same function in each type of microorganism, particular antibody to ribosomal protein, that is, ribosomal protein L7/L12, is made and antibody that reacts specifically with the microorganism in question is selected.
    Type: Application
    Filed: April 15, 2009
    Publication date: October 29, 2009
    Applicant: ASAHI KASEI KOGYO KABUSHIKI KAISHA
    Inventors: Kenji Matsuyama, Takashi Shirai, Takashi Etoh, Monzur Rahman