Pseudomonas Putida Patents (Class 435/877)
  • Patent number: 8187862
    Abstract: A method and composition for bioremediation of environmental material. In one embodiment, material to be bioremediated is debris is obtained from a metropolitan street cleaning operation. An environmentally safe all natural non-pathogenic microbial composition is used under conditions sufficient to bioremediate the material.
    Type: Grant
    Filed: March 6, 2007
    Date of Patent: May 29, 2012
    Assignee: CL Solutions
    Inventors: Michael T. Saul, Efrain Torres, Peter A. Vandenbergh
  • Patent number: 7906315
    Abstract: Novel strains of isolated and purified bacteria have been identified which have the ability to degrade petroleum hydrocarbons including a variety of PAHs. Several isolates also exhibit the ability to produce a biosurfactant. The combination of the biosurfactant-producing ability along with the ability to degrade PAHs enhances the efficiency with which PAHs may be degraded. Additionally, the biosurfactant also provides an additional ability to bind heavy metal ions for removal from a soil or aquatic environment.
    Type: Grant
    Filed: June 7, 2007
    Date of Patent: March 15, 2011
    Assignee: Savannah River Nuclear Solutions, LLC
    Inventors: Robin L. Brigmon, Sandra Story, Denis J. Altman, Christopher J. Berry
  • Patent number: 7473546
    Abstract: Novel strains of isolated and purified bacteria have been identified which have the ability to degrade petroleum hydrocarbons including a variety of PAHs. Several isolates also exhibit the ability to produce a biosurfactant. The combination of the biosurfactant-producing ability along with the ability to degrade PAHs enhances the efficiency with which PAHs may be degraded. Additionally, the biosurfactant also provides an additional ability to bind heavy metal ions for removal from a soil or aquatic environment.
    Type: Grant
    Filed: November 13, 2003
    Date of Patent: January 6, 2009
    Assignee: Savannah River Nuclear Solutions, LLC
    Inventors: Robin L. Brigmon, Sandra Story, Denis Altman, Christopher J. Berry
  • Patent number: 7070983
    Abstract: The present invention relates to mutant strains of Pseudomonas putida and their use in the detoxification of industrial and/or toxic waste products.
    Type: Grant
    Filed: October 9, 2003
    Date of Patent: July 4, 2006
    Assignee: National Food Research Institute
    Inventors: Kozo Ochi, Keiichi Hosokawa
  • Patent number: 6913911
    Abstract: Transgenic microbial strains are provided which contain the genes required for PHA formation integrated on the chromosome. The strains are advantageous in PHA production processes, because (1) no plasmids need to be maintained, generally obviating the required use of antibiotics or other stabilizing pressures, and (2) no plasmid loss occurs, thereby stabilizing the number of gene copies per cell throughout the fermentation process, resulting in homogeneous PHA product formation throughout the production process. Genes are integrated using standard techniques, preferably transposon mutagenesis. In a preferred embodiment wherein mutiple genes are incorporated, these are incorporated as an operon. Sequences are used to stabilize mRNA, to induce expression as a function of culture conditions (such as phosphate concentration), temperature, and stress, and to aid in selection, through the incorporation of selection markers such as markers conferring antibiotic resistance.
    Type: Grant
    Filed: June 13, 2003
    Date of Patent: July 5, 2005
    Assignee: Metabolix, Inc.
    Inventors: Gjalt W. Huisman, Oliver P. Peoples, Frank A. Skraly
  • Patent number: 6835559
    Abstract: A process for producing an optical active &bgr;-amino alcohol, the method comprising the step of allowing at least one microorganism selected from the group consisting of microorganisms belonging to the genus Morganella and others, to act on an enantiomeric mixture of an &agr;-aminoketone or a salt thereof having the general formula (I): to produce an optical active &bgr;-amino alcohol with the desired optical activity having the general formula (II) described below in a high yield as well as in a highly selective manner:
    Type: Grant
    Filed: January 22, 2003
    Date of Patent: December 28, 2004
    Assignee: Daiichi Fine Chemical Co., Ltd.
    Inventors: Keiji Sakamoto, Shinji Kita, Kazuya Tsuzaki, Tadanori Morikawa, Sakayu Shimizu, Michihiko Kataoka
  • Patent number: 6803444
    Abstract: Polyhydroxyalkanoate type polyester that comprises one unit % or more of 3-hydroxy-&ohgr;-(4-vinylphenyl)alkanoic acid units. A microbial production method is also provided.
    Type: Grant
    Filed: February 28, 2002
    Date of Patent: October 12, 2004
    Assignee: Canon Kabushiki Kaisha
    Inventors: Tomohiro Suzuki, Etsuko Sugawa, Tetsuya Yano, Tsuyoshi Nomoto, Takeshi Imamura, Tsutomu Honma, Takashi Kenmoku
  • Patent number: 6777153
    Abstract: A polyhydroxyalkanoate characterized by having in the molecule a unit represented by Chemical Formula (1): wherein n may assume any one integral value within the range of from 1 to 8. Also disclosed are a process for producing the polyhydroxyalkanoate by the use of a microorganism having the ability to produce the polyhydroxyalkanoate and accumulate it in the bacterial body; a charge control agent, a toner binder and a toner which contain this polyhydroxyalkanoate; and an image-forming method and an image-forming apparatus which make use of the toner.
    Type: Grant
    Filed: March 26, 2002
    Date of Patent: August 17, 2004
    Assignee: Canon Kabushiki Kaisha
    Inventors: Tetsuya Yano, Etsuko Sugawa, Takeshi Imamura, Tsutomu Honma, Takashi Kenmoku
  • Patent number: 6762047
    Abstract: A composition useful for cleaning and bioremediation applications is provided which comprises a Pseudomonas species, an alkali metal nitrate, an ethoxylate nonionic surfactant, and optionally monoammonium phosphate as a buffer in an aqueous solution. The Pseudomonas sp. is stable during storage of the composition.
    Type: Grant
    Filed: June 24, 2002
    Date of Patent: July 13, 2004
    Assignee: Osprey Biotechnics, Inc.
    Inventor: Peter A. Vandenbergh
  • Patent number: 6686439
    Abstract: PHA containing a novel 3-hydroxy-thioalkanoic acid unit having a highly reactive thienyl group in a side chain thereof, and a method of producing the same are provided. Specifically, 5-(2-thienyl-sulfanyl)valeric acid represented by Chemical Formula [4] below and 6-(2-thienylsulfanyl)hexanoic acid represented by Chemical Formula [5] below are provided. Further, a method of producing PHA, comprising the step of collecting PHA from cells of a microorganism cultured in a medium containing the valeric acid or hexanoic acid, and a novel PHA represented by Chemical Formula [1] below are provided.
    Type: Grant
    Filed: February 28, 2002
    Date of Patent: February 3, 2004
    Assignee: Canon Kabushiki Kaisha
    Inventors: Takashi Kenmoku, Etsuko Sugawa, Tetsuya Yano, Tsuyoshi Nomoto, Takeshi Imamura, Tomohiro Suzuki, Tsutomu Honma
  • Patent number: 6645743
    Abstract: A polyhydroxyalkanoate (PHA) having a desired configuration is produced using a raw material containing &ohgr;-(4-vinylphenyl)-alkanoic acid and (&ohgr;-substituted alkanoic acid in which a group having a ring structure selected from phenyl, thienyl, and cyclohexyl structures substitutes therefor on the end thereof by producing a PHA copolymer containing the corresponding 3-hydroxy-&ohgr;-(4-vinylphenyl)-alkanoate unit and the corresponding 3-hydroxy-&ohgr;-substituted alkanoate unit by making use of a microorganism capable of producing the PHA or by oxidizing a predetermined portion of the corresponding PHA.
    Type: Grant
    Filed: February 25, 2003
    Date of Patent: November 11, 2003
    Assignee: Canon Kabushiki Kaisha
    Inventors: Tsutomu Honma, Etsuko Sugawa, Tetsuya Yano, Takeshi Imamura, Takashi Kenmoku, Tatsuki Fukui
  • Patent number: 6593116
    Abstract: Transgenic microbial strains are provided which contain the genes required for PHA formation integrated on the chromosome. The strains are advantageous in PHA production processes, because (1) no plasmids need to be maintained, generally obviating the required use of antibiotics or other stabilizing pressures, and (2) no plasmid loss occurs, thereby stabilizing the number of gene copies per cell throughout the fermentation process, resulting in homogeneous PHA product formation throughout the production process. Genes are integrated using standard techniques, preferably transposon mutagenesis. In a preferred embodiment wherein mutiple genes are incorporated, these are incorporated as an operon. Sequences are used to stabilize mRNA, to induce expression as a function of culture conditions (such as phosphate concentration), temperature, and stress, and to aid in selection, through the incorporation of selection markers such as markers conferring antibiotic resistance.
    Type: Grant
    Filed: August 17, 1999
    Date of Patent: July 15, 2003
    Assignee: Metabolix, Inc.
    Inventors: Gjalt W. Huisman, Oliver P. Peoples, Frank A. Skraly
  • Patent number: 6492147
    Abstract: A method for producing a microbial polyester by culturing a microorganism being capable of producing a poly hydroxyalkanoate polyester in a culture medium containing 1-hexene as a sole carbon source.
    Type: Grant
    Filed: December 20, 2000
    Date of Patent: December 10, 2002
    Assignee: Canon Kabushiki Kaisha
    Inventors: Takeshi Imamura, Tetsuya Yano, Shin Kobayashi, Sakae Suda, Tsutomu Honma
  • Patent number: 6479621
    Abstract: Microorganisms capable of synthesizing novel polyhydroxyalkanoate having 3-hydroxythienylalkanoic acid as monomer unit, using thienylalkanoic acid as a stock are cultured on a culture medium containing thienylalkanoic acid, and the polyhydroxyalkanoate produced in the cultured cell is extracted and
    Type: Grant
    Filed: February 28, 2001
    Date of Patent: November 12, 2002
    Assignee: Canon Kabushiki Kaisha
    Inventors: Tsutomu Honma, Tetsuya Yano, Shin Kobayashi, Takeshi Imamura, Takashi Kenmoku, Shinya Kozaki
  • Patent number: 6410265
    Abstract: This invention relates to the isolation of a novel putative efflux gene from Pseudomonas mendocina. The putative efflux gene is useful for probing an organism's efflux system to gain an understanding of the mechanisms of solvent tolerance. The invention further provides a Pseudomonas mendocina strain deficient in this gene. This strain is unable to grow in the presence of chloramphenicol and, compared to the wildtype strain, grows slowly in the presence of high concentrations of PHBA.
    Type: Grant
    Filed: October 30, 1998
    Date of Patent: June 25, 2002
    Assignee: E. I. du Pont de Nemours and Company
    Inventor: Kevin Keqin Chen
  • Patent number: 6361979
    Abstract: The present invention relates to processes for the microbial oxidation of 2-methylquinoxaline to 2-quinoxalinecarboxylic acid which comprise contacting 2-methylquinoxaline with a microorganism, or a suitable mutant thereof, and incubating the resulting mixture under conditions sufficient to yield an amount of said 2-quinoxalinecarboxylic acid. The present processes optionally further comprise the isolation and purification of 2-quinoxalinecarboxylic acid.
    Type: Grant
    Filed: January 27, 2000
    Date of Patent: March 26, 2002
    Assignee: Pfizer Inc.
    Inventors: Michael P. Burns, James J. Cawley, John W. Wong
  • Patent number: 6309861
    Abstract: A process produces a water-soluble, naturally folded eukaryotic polypeptide containing two or several cysteines linked by disulfide bridges. This process involves culturing prokaryotic cells, a) in which the prokaryotic cells contain an expression vector which encodes the polypeptide which contains a prokaryotic signal sequence at the N-terminus, b) under conditions under which the polypeptide is secreted into the periplasm or the medium, c) cleaving the signal sequence and isolating the polypeptide from the periplasm or the medium. In this process, the culturing is carried out in the presence of arginine or a compound of the formula I R2—CO—NR1 (I) in which R and R1 represent hydrogen or a saturated or unsaturated branched or unbranched C1-C4 alkyl chain and R2 represents hydrogen, NHR1 or a saturated or unsaturated branched or unbranched C1-C3 alkyl chain, is suitable for the recombinant production of polypeptides in prokaryotes in a high yield.
    Type: Grant
    Filed: April 20, 2000
    Date of Patent: October 30, 2001
    Assignee: Hoffmann-La Roche Inc.
    Inventors: Dorothee Ambrosius, Rainer Rudolph, Joerg Schaeffner, Elisabeth Schwarz
  • Patent number: 6284523
    Abstract: A process for biodegradation of a xenobiotic. A process for biodegradation of a xenobiotic comprising the steps of: contacting an organic phase comprising the xenobiotic and at least one substantially water-immiscible organic solvent with an aqueous phase comprising water and a microorganism capable of metabolizing the xenobiotic; partitioning a portion of the xenobiotic from the organic phase to the aqueous phase such that the concentration of the xenobiotic in the aqueous phase is substantially non-toxic to the microorganism; and causing the microorganism to metabolize the xenobiotic in the aqueous phase.
    Type: Grant
    Filed: September 18, 1998
    Date of Patent: September 4, 2001
    Inventors: Andrew J. Daugulis, Lisa D. Collins
  • Patent number: 6265191
    Abstract: Lipase is immobilized on surfaces to facilitate oil removal from the surfaces and to alter wettability of the surfaces. The lipase is isolatable from a Pseudomonas organism such as Pseudomonas putida ATCC 53552 or from an organism expressing a coding region found in or cloned from the Pseudomonas. A particularly preferred lipase has a molecular weight of about 30 to 35 kd and is resolvable as a single band by SDS gel electrophoresis. Lipase sorbed on fabric forms a fabric-lipase complex for oil stain removal. The lipase may be sorbed on fabric before or after an oil stain, and the lipase is active to hydrolyze an oil stain on dry fabric or fabric in laundering solutions. The sorbed lipase has enhanced stability to denaturation by surfactants and to heat deactivation, is resistant to removal from fabric during laundering, retains substantial activity after drying fabric at an elevated temperature, and retains activity during fabric storage or wear.
    Type: Grant
    Filed: August 20, 1993
    Date of Patent: July 24, 2001
    Assignee: The Clorox Company
    Inventors: Eugene A. Mizusawa, Susan A. Anderson, Maha Y. El-Sayed, Daniel R. Leiske, Richard J. Wiersema, Chihae Yang
  • Patent number: 6165777
    Abstract: A process for resolution of a mixture of dihydroxydihydroindene enantiomers comprising treating the mixture of enantiomers with a Pseudomonas putida species microorganism.Resolved dihydroxydihydroindenes are valuable intermediates for the synthesis of biologically active compounds such as pharmaceuticals and agrochemicals.
    Type: Grant
    Filed: June 9, 1998
    Date of Patent: December 26, 2000
    Assignee: Zeneca Limited
    Inventors: Christopher Curtis Royston Allen, Derek Raymond Boyd, Howard Dalton
  • Patent number: 6136589
    Abstract: The present invention relates to novel strains of Pseudomonas putida, more specifically, to a novel strain of Pseudomonas putida which are tolerant to organic solvents including hydrocarbons, alcohols, ethers, ketones and their derivatives or mixtures thereof, and a mutant strain of said microorganism having the availability of genetic manipulation. The said Pseudomonas putida strains can be used in biotransformation in the presence of organic solvents and bioremediation of toxic organic compounds. Furthermore, they are useful as supply sources of resistance genes and cell fusion of organic solvent-tolerant microorganisms producing useful substances and the breeding of said microorganisms can be practiced in the art, which allows their universal use in the fields of bioreactor, liquid-waste treatment, protein engineering, etc.
    Type: Grant
    Filed: July 9, 1999
    Date of Patent: October 24, 2000
    Assignee: Samsung Electronics Co. Ltd
    Inventors: Dong-Bin Lim, Kwang Kim, Sung-Jin Lee, Kyung-Hee Lee
  • Patent number: 6133421
    Abstract: 350 The present invention provides a nitrile hydratase nucleic acid fragment isolated from Pseudomonas putida which encodes a nitrile hydratase activity capable of catalyzing the hydrolysis of certain racemic nitriles to the corresponding R- or S-amides. Also provided are transformed microorganisms capable of the active expression of said nitrile hydratase activity. Additionally, the invention provides a transformant harboring the nitrile hydratase gene in conjunction with an amidase gene, both of which may be co-expressed producing active nitrile hydratase and amidase enzymes respectively. Methods for the production of such enantiomeric materials are also provided.
    Type: Grant
    Filed: June 24, 1998
    Date of Patent: October 17, 2000
    Assignee: E. I. du Pont de Nemours & Company
    Inventors: Robert Donald Fallon, Mark James Nelson, Mark Scott Payne
  • Patent number: 5981210
    Abstract: The method of the invention involves providing a first receptacle and a second receptacle. The first receptacle contains a sterile aqueous broth and the second receptacle contains an aqueous broth including a carbon source. The method then includes placing into the first receptacle a first support surface having a paraffin wax coating thereon and placing into the second receptacle a second support surface having a hydrophobic material coating thereon. A body specimen, such as sputum, is then introduced into each of the first and second receptacles. The presence of a nonparaffinophilic hydrophobic microorganism in the body specimen is determined by observing (i) a lack of microorganism growth on the paraffin coated material of the first support surface and (ii) a presence of microorganism growth on the hydrophobic material coating of the second support surface. The presence of the nonparaffinophilic hydrophobic microorganism can be further confirmed by performing a DNA extraction.
    Type: Grant
    Filed: July 24, 1998
    Date of Patent: November 9, 1999
    Assignee: Infectech, Inc.
    Inventor: Robert A. Ollar
  • Patent number: 5980747
    Abstract: A composition and method for preserving Pseudomonads is described. The composition includes an alkali metal nitrate, and optionally monoammonium phosphate as a buffer in an aqueous solution to preserve the bacteria. The compositions are useful for bioremediation.
    Type: Grant
    Filed: March 17, 1997
    Date of Patent: November 9, 1999
    Assignee: Osprey Biotechnics, Inc.
    Inventors: Peter A. Vandenbergh, Blair S. Kunka, Hiren K. Trivedi
  • Patent number: 5965411
    Abstract: An enzyme, which has a molecular weight of about 57,000-120,000 daltons on SDS-PAGE and a pI of about 3.8-5.1 on isoelectrophoresis using ampholyte, converts maltose into trehalose and vice versa. The enzyme was isolated from microorganisms of the genera Pimelobacter, Pseudomonas and Thermus. By using the enzyme, trehalose is readily formed from a commercially available maltose in an industrial scale and a relatively-low cost. Trehalose and saccharide compositions containing the same, which are preparable with the enzyme, are suitably used in food products, cosmetic compositions and pharmaceutical compositions.
    Type: Grant
    Filed: November 4, 1997
    Date of Patent: October 12, 1999
    Assignee: Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
    Inventors: Tomoyuki Nishimoto, Hiroto Chaen, Toshiyuki Sugimoto, Toshio Miyake
  • Patent number: 5948671
    Abstract: A biologically pure culture of Pseudomonas putida NRRL B-30041 is described which is highly effective as a biological control agent against replant disease in tree fruits. The invention also encompasses methods of biologically controlling replant disease using the bacterium of the invention, and agricultural compositions which incorporate the strain.
    Type: Grant
    Filed: July 24, 1998
    Date of Patent: September 7, 1999
    Assignee: The United States of America as represented by the Secretary of Agriculture
    Inventor: Mark Mazzola
  • Patent number: 5888785
    Abstract: The present invention provides a nitrile hydratase nucleic acid fragment isolated from Pseudomonas putida which encodes a nitrile hydratase activity capable of catalyzing the hydrolysis of certain racemic nitrites to the corresponding R- or S-amides. Also provided are transformed microorganisms capable of the active expression of said nitrile hydratase activity. Additionally, the invention provides a transformant harboring the nitrile hydratase gene in conjunction with an amidase gene, both of which may be co-expressed producing active nitrile hydratase and amidase enzymes respectively. Methods for the production of such enantiomeric materials are also provided.
    Type: Grant
    Filed: June 24, 1998
    Date of Patent: March 30, 1999
    Assignee: E. I. du Pont de Nemours and Company
    Inventors: Robert Donald Fallon, Mark James Nelson, Mark Scott Payne
  • Patent number: 5858737
    Abstract: A process is disclosed that bioconverts indene to (1S)-amino-(2R)-indanol substantially free of any of its stereoisomers, by the action of a strain of P. putida or Rhodococcus sp., followed by various chemical step(s), e.g., chiral specific crystallization, treatment with strong acid in the presence of acetonitrile.
    Type: Grant
    Filed: August 14, 1996
    Date of Patent: January 12, 1999
    Assignee: Merck & Co., Inc.
    Inventors: Barry C. Buckland, Neal C. Connors, Michel M. Chartrain, Francis P. Gailliot, Randolph L. Greasham, Barbara Jackey, Brian Heimbuch, Chanyong Lee, Roger C. Olewinski, Jr., F. Edward Roberts, Paul J. Reider, Thomas R. Verhoeven, Chris H. Senanayake
  • Patent number: 5824540
    Abstract: A process is disclosed that bioconverts indene to (1S)-amino-(2R)-indanol substantially free of any of its stereoisomers, by the action of the enzyme dioxygenase, followed by various chemical step(s), e.g., chiral specific crystallization, treatment with strong acid in the presence of acetonitrile.
    Type: Grant
    Filed: June 18, 1996
    Date of Patent: October 20, 1998
    Assignee: Merck & Co., Inc.
    Inventor: Neal C. Connors
  • Patent number: 5811294
    Abstract: A process for resolution of a mixture of cis-dihydroxydihydroindene enantiomers comprising treating the mixture of enantiomers with a Pseudomonas putida species microorganism.Resolved dihydroxydihydroindenes are valuable intermediates for the synthesis of biologically active compounds such as pharmaceuticals and agrochemicals.
    Type: Grant
    Filed: April 4, 1997
    Date of Patent: September 22, 1998
    Assignee: Zeneca Limited
    Inventors: Christopher Curtis Royston Allen, Derek Raymond Boyd, Howard Dalton
  • Patent number: 5811286
    Abstract: The present invention provides a nitrile hydratase nucleic acid fragment isolated from Pseudomonas putida which encodes a nitrile hydratase activity capable of catalyzing the hydrolysis of certain racemic nitriles to the corresponding R- or S-amides. Also provided are transformed microorganisms capable of the active expression of said nitrile hydratase activity. Additionally, the invention provides a transformant harboring the nitrile hydratase gene in conjunction with an amidase gene, both of which may be co-expressed producing active nitrile hydratase and amidase enzymes respectively. Methods for the production of such enantiomeric materials are also provided.
    Type: Grant
    Filed: October 4, 1996
    Date of Patent: September 22, 1998
    Assignee: E. I. du Pont de Nemours and Company
    Inventors: Robert Donald Fallon, Mark James Nelson, Mark Scott Payne
  • Patent number: 5804435
    Abstract: The present invention provides a method for obtaining organic solvent resistant microorganisms which comprises subjecting a microbial parent strain to mutagenesis and then to selective cultivation in the presence of 0.1% to 10% by volume (v/v) of concentrations of a toxic organic solvent, and the organic solvent resistant microorganisms obtainable by the method. In addition according to the present invention, a microorganism which is natively hydrophilic and has useful functions but does not show resistance to organic solvents and can not express the useful functions in the organic solvents may be converted into a microorganism capable of growing in the presence of such toxic organic solvents and expressing the useful functions that the hydrophilic parent strain bears natively.
    Type: Grant
    Filed: October 29, 1996
    Date of Patent: September 8, 1998
    Assignees: Tonen Corporation, The Agency of Industrial Science and Technology
    Inventors: Ryuichiro Kurane, Takuichi Tsubata
  • Patent number: 5766893
    Abstract: Microorganisms of interest are capable of utilizing .alpha.-imino carboxamides, in the form of the racemate or of its optically active isomers, of the general formula ##STR1## wherein A together with --NH-- and --CH-- is an optionally substituted 5- or 6-membered saturated heterocyclic ring, as sole nitrogen source, and converting (RS)-.alpha.-imino carboxamides of Formula I into an S-.alpha.-imino carboxylic acid of the general formula ##STR2## These microorganisms are useful also for biconversion of an (RS)-.alpha.-imino carboxamide of Formula I into an S-.alpha.-imino carboxylic acid.
    Type: Grant
    Filed: March 12, 1996
    Date of Patent: June 16, 1998
    Assignee: Lonza AG
    Inventors: Andreas Kiener, Jean-Paul Roduit, Jorg Kohr, Nicholas Shaw
  • Patent number: 5763233
    Abstract: This invention provides a process for the production of .delta.-decalactone by the microbial reduction of massoia lactone, characterized in that a bacterium having the ability to reduce massoia lactone is used as the microorganism. The .delta.-decalactone produced according to this process has a highly tastable, mild creamlike scent and flavor, and is hence suitable for use in flavor compositions.
    Type: Grant
    Filed: July 31, 1997
    Date of Patent: June 9, 1998
    Assignee: T. Hasegawa Co., Ltd.
    Inventors: Shinobu Gocho, Kitazawa Rumi, Komai Tsuyoshi
  • Patent number: 5744321
    Abstract: A rapid, on-site method for indicating the degree of spoilage, if any, of finfish by the level of bacteria present therein. A small quantity of flesh is cut from a representative fish and kneaded in a bacterial nutrient broth to extract any bacteria present. A triphenyl tetrazolium dye is added as an indicator reagent, followed by an anionic surfactant and a lower alkyl alcohol. The developed color, if any, is compared to a control color chart representative of acceptable and unsatisfactory degrees of bacterial contamination or spoilage.
    Type: Grant
    Filed: February 21, 1997
    Date of Patent: April 28, 1998
    Assignee: GEM Biomedical, Inc.
    Inventor: Patrick Harewood
  • Patent number: 5736380
    Abstract: An enzyme, which has a molecular weight of about 57,000-120,000 daltons on SDS-PAGE and a pI of about 3.8-5.1 on isoelectrophoresis using ampholyte, converts maltose into trehalose and vice versa. The enzyme was isolated from microorganisms of the genera Pimelobacter, Pseudomonas and Thermus. By using the enzyme, trehalose is readily formed from a commercially available maltose in an industrial scale and a relatively-low cost. Trehalose and saccharide compositions containing the same, which are preparable with the enzyme, are suitably used in food products, cosmetic compositions and pharmaceutical compositions.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: April 7, 1998
    Assignee: Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
    Inventors: Tomoyuki Nishimoto, Hiroto Chaen, Toshiyuki Sugimoto, Toshio Miyake
  • Patent number: 5728556
    Abstract: Applicants have provided methods for obtaining aliphatic omega-cyanocaboximides of Formula INC--CH(R.sub.1)(CH).sub.n CH(R.sub.2)C(O)NH.sub.2whereinn=1-8 and R.sub.1 or R.sub.2 are either H or CH.sub.3,from dinitriles of Formula IINC--CH(R.sub.1)(CH).sub.n CH(R.sub.2)CNwhereinn=1-8 and R.sub.1 or R.sub.2 are either H or CH.sub.3,using biocatalysts which have regioselective nitrile hydratase activity and which are derived from members of the bacterial species Pseudomonas putida.
    Type: Grant
    Filed: March 14, 1996
    Date of Patent: March 17, 1998
    Assignee: E. I. du Pont de Nemours and Company
    Inventors: Robert DiCosimo, Barry Stieglitz, Robert D. Fallon
  • Patent number: 5693527
    Abstract: A process is disclosed in which a chloroorganic compound is decomposed by means of a microorganism. The relevant microorganisms are induced to do so by contact with an aromatic compound which may be extracted from a plant containing lignocellulose or may be p-coumaric acid. It is believed that these compounds stimulate the microorganism to express oxygenase, and that the presence of oxygenase in the microroganism enables it to decompose the chlororoganic compound.
    Type: Grant
    Filed: December 21, 1994
    Date of Patent: December 2, 1997
    Assignee: Canon Kabushiki Kaisha
    Inventor: Takeshi Imamura
  • Patent number: 5672504
    Abstract: A microorganism or a preparation thereof is permitted to act on a mixture of enantiomers of an epoxide such as 3-chlorostyrene oxide and the product optically active epoxide is recovered. The microorganism able to produce an optically active (S)-epoxide from the mixture of enantiomers of the epoxide include, for example, a microorganism strain belonging to the genus Candida, the genus Rhodosporidium, the genus Rhodococcus and the genus Nosardioides. Examples of the microorganism capable of producing an optically active (R)-epoxide from said mixture include a microorganism strain belonging to the genus Trichosporon, the genus Geotrichum, the genus Corynebacterium, the genus Micrococcus and the genus Brevibacterium. The objective optically active epoxide can efficiently be obtained with ease and simplicity from the corresponding mixture of enantiomers of the epoxide.
    Type: Grant
    Filed: October 25, 1995
    Date of Patent: September 30, 1997
    Assignee: Daicel Chemical Industries, Ltd.
    Inventors: Akinobu Matsuyama, Yoshinori Kobayashi
  • Patent number: 5622846
    Abstract: Microorganisms of interest are capable of utilizing .alpha.-imino carboxamides, in the form of the racemate or of its optically active isomers, of the general formula ##STR1## wherein A together with --NH-- and --CH-- is an optionally substituted 5- or 6-membered saturated heterocyclic ring, as sole nitrogen source, and converting (RS)-.alpha.-imino carboximides of Formula I into an S-.alpha.-amino carboxylic acid of the general formula ##STR2## These microorganisms are useful also for biconversion of an (RS)-.alpha.-imino carboxamide of Formula I into an S-.alpha.-amino carboxylic acid.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: April 22, 1997
    Assignee: Lonza AG
    Inventors: Andreas Kiener, Jean-Paul Roduit, J org Kohr, Nicholas Shaw
  • Patent number: 5607848
    Abstract: The present invention provides a process for producing an optically active 4-hydroxy-2-ketoglutaric acid, by mixing glyoxylic acid, pyruvic acid and a microorganism to form the optically active 4-hydroxy-2-ketoglutaric acid in an aqueous medium.
    Type: Grant
    Filed: February 28, 1995
    Date of Patent: March 4, 1997
    Assignee: Kyowa Hakko Kogyo Co., Ltd.
    Inventors: Ryoichi Katsumata, Shinichi Hashimoto, Keiko Ochiai
  • Patent number: 5597716
    Abstract: The present invention relates to a process for producing D-lactic acid and L-lactamide, comprising allowing a culture broth of a microorganism capable of asymmetric hydrolysis of DL-lactamide belonging to the genus Alcaligenes, Pseudomonas, Agrobacterium, Brevibacterium, Acinetobacter, Corynebacterium, Enterobacter, Micrococcus or Rhodococcus, the microorganism itself, a material obtained therefrom or an immobilized material thereof to act on DL-lactamide, and recovering the resulting D-lactic acid and the remaining L-lactamide. The present invention enables sufficient production of D-lactic acid and L-lactamide by the present microorganism.
    Type: Grant
    Filed: November 17, 1994
    Date of Patent: January 28, 1997
    Assignee: Mitsubishi Rayon Co., Ltd.
    Inventors: Eiji Sato, Eiji Ozaki, Chinami Iida, Yoshimasa Kobayashi, Akihiro Sakimae
  • Patent number: 5593865
    Abstract: A process for the activation of disulphide linked recombinant proteins expressed in prokaryotes is described. The process includes cell digestion, solubilization under denaturing and reducing conditions and activation under oxidizing conditions in the presence of GSH/GSSG and a non-denaturing amount of a denaturing agent.
    Type: Grant
    Filed: June 1, 1995
    Date of Patent: January 14, 1997
    Assignee: Boehringer Mannheim GmbH
    Inventors: Rainer Rudolph, Stephan Fischer, Ralf Mattes
  • Patent number: 5580746
    Abstract: Salicylate hydroxylase isolated from Pseudomonas bacteria can be used to determine the level of salicylate in a body fluid by reacting a sample of the fluid with the enzyme and monitoring the conversion of salicylate to catechol. A method of purifying the enzyme from crude bacterial extract using a salicylate affinity column is also disclosed.
    Type: Grant
    Filed: November 7, 1994
    Date of Patent: December 3, 1996
    Inventor: Kwan-sa You
  • Patent number: 5575998
    Abstract: The mixture of natural microorganisms causes a biodegradation of mineral oils and mineral oil products. It contains Pseudomonas putida and Geotrichum candidum in a ratio of the cell-numbers of 5:1 to 1:1. The mixture is induced by a cultivation in the presence of oil-acid. Afterwards, one works with it on hydrocarbons under the supply of oxygen as well as in the presence of stimulation-substances at a pH-value of 4.5 to 7.5 and at a temperature of 5.degree.+C. to 35.degree. C.
    Type: Grant
    Filed: November 21, 1994
    Date of Patent: November 19, 1996
    Assignee: Biorem AG
    Inventors: Miroslav Nemec, Dana Horakova
  • Patent number: 5545547
    Abstract: The invention relates to a method of increasing the effect of agricultural chemicals comprising treating a plant with a plant depolymerase enzyme that will degrade plant surface polymers either prior to, or concurrently with administration of the agricultural chemical enzyme.
    Type: Grant
    Filed: April 10, 1992
    Date of Patent: August 13, 1996
    Inventors: Pappachan Kolattukudy, Ayrookaran J. Poulose
  • Patent number: 5503652
    Abstract: Bacterial strains can be reproducibly isolated from soil that are root-colonizing and directly promote plant development. For example, strains of soil bacteria are provided that are good root colonizers and that promote plant growth under gnotobiotic conditions.
    Type: Grant
    Filed: January 31, 1995
    Date of Patent: April 2, 1996
    Assignee: Cominco Fertilizers
    Inventors: Joseph W. Kloepper, Catherine Simonson, Ran Lifshitz
  • Patent number: 5503651
    Abstract: Bacterial strains can be reproducibly isolated from the rhizosphere that enhance yield in nonroot crops under field conditions.
    Type: Grant
    Filed: January 31, 1995
    Date of Patent: April 2, 1996
    Assignee: Cominco Fertilizers
    Inventors: Joseph Kloepper, Fran Scher
  • Patent number: 5453363
    Abstract: A process for the activation of t-PA or IgG after expression in prokaryotes is described. The process includes cell digestion, solubilization under denaturing and reducing conditions and activation under oxidizing conditions in the presence of GSH/GSSG.
    Type: Grant
    Filed: March 2, 1994
    Date of Patent: September 26, 1995
    Assignee: Boehringer Mannheim GmbH
    Inventors: Rainer Rudolph, Stephan Fischer, Ralf Mattes
  • Patent number: 5436145
    Abstract: 6-Hydroxy nitrogen-containing 6-membered ring compounds of the following general formula (II): ##STR1## wherein R.sup.1 represents carboxy group, carbamoyl group, cyano group, formyl group, C.sub.1 -C.sub.5 hydroxyalkyl group, C.sub.2 -C.sub.6 alkoxycarbonyl group, carboxyvinyl group, carboxymethyl group or oxime group, R.sup.2 represents hydrogen atom or carboxy group, and A represents carbon atom or nitrogen atom, can be prepared by reacting a nitrogen-containing 6-membered ring compounds of the following general formula (I): ##STR2## wherein R.sup.1, R.sup.2 and A are as defined in the general formula (II) above, with a microorganism or physico-chemically treated microorganism in an aqueous medium. Efficiency of the above reaction can be raised by conducting the reaction in the presence of phenazine methosulfate.
    Type: Grant
    Filed: May 20, 1994
    Date of Patent: July 25, 1995
    Assignee: Mitsubishi Kasei Corporation
    Inventors: Mari Yasuda, Haruyuki Ohkishi, Katsutoshi Sato, Yuuki Morimoto, Toru Nagasawa