Produced By The Action Of An Isomerase (e.g., Fructose By The Action Of Xylose Isomerase On Glucose, Etc.) Patents (Class 435/94)
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Patent number: 10851429Abstract: The present invention relates to a granular form of mixed saccharide composition comprising a mixed saccharide containing psicose as a main ingredient and a method for preparing the same, and more particularly to a granule of a mixed saccharide containing psicose with improved sweetness quality and physical properties and a method for preparing the same.Type: GrantFiled: April 28, 2016Date of Patent: December 1, 2020Assignee: SAMYANG CORPORATIONInventors: Go-Eun Kim, Sung Won Park, Su Youn Lim, Hye Jung Kim, Chong Jin Park, Kang Pyo Lee
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Patent number: 10745720Abstract: A method for producing tagatose comprises: a) performing epimerization of fructose using hexuronate C4-epimerase to obtain an epimerized product comprising tagatose; b) purifying the epimerized product; and c) crystallizing the purified epimerized product. The hexuronate C4-epimerase is an enzyme derived from Thermotoga maritima, Thermotoga neapolitana, Thermotoga thermarum or mutants thereof. The hexuronate C4-epimerase is produced from strains Escherichia coli, Corynebacterum glutamicum, Aspergillus oryzae, or Bacillus subtilis.Type: GrantFiled: June 3, 2014Date of Patent: August 18, 2020Assignee: CJ CHEILJEDANG CORPORATIONInventors: Sung Jae Yang, Yang Hee Kim, Seong Bo Kim, Seung Won Park, Il Hyang Park, Min Hae Kim, Young Mi Lee
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Patent number: 10736346Abstract: The present invention provides a method for producing a solid roux which has excellent shape retention properties even in summer, does not undergo seepage of liquid oily components to the surface or whitening of the surface, melts well in the mouth and has a reduced content of trans fatty acids and saturated fatty acids. A solid roux base, which contains specified quantities of table sugar and one or more types of sugar selected from among maltose, trehalose, fructose, palatinose, reduced palatinose, maltitol, erythritol, lactitol and sorbitol, is solidified by means of heat treatment at 100-160° C. In addition, solid oils and fats having a relatively low content of trans fatty acids and saturated fatty acids and having melting points of 40° C. or lower are used as the oils and fats for the solid roux.Type: GrantFiled: January 20, 2016Date of Patent: August 11, 2020Assignee: FUJI OIL HOLDINGS INC.Inventor: Hitomi Omori
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Patent number: 10544409Abstract: A hexuronate C4-epimerase with improved conversion activity and a method for producing D-tagatose using the hexuronate C4-epimerase. The hexuronate C4-epimerase includes an amino acid sequence set forth in SEQ ID NO: 1, in which serine (S) at position 125, serine (S) at position 185, valine (V) at position 267, serine (S) at position 268, threonine (T) at position 272, tryptophan (W) at position 306, arginine (R) at position 386 and tyrosine (Y) at position 403 from an N-terminal of hexunorate C4-epimerase are mutated.Type: GrantFiled: December 21, 2018Date of Patent: January 28, 2020Assignee: CJ Cheiljedang CorporationInventors: Sung Jae Yang, Yang Hee Kim, Il Hyang Park, Young Mi Lee, Hyun Kug Cho, Seong Bo Kim, Seung Won Park
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Patent number: 10463064Abstract: The invention relates to a process for purifying syrups. The process comprises removing charged components from the syrup by passing said syrup through a capacitive deionization cell. The present invention further relates to the use of capacitive deionization to recover charged components from syrups. In particular the syrups are mannose, fructose and sorbitol containing syrups.Type: GrantFiled: March 5, 2014Date of Patent: November 5, 2019Assignee: CARGILL, INCORPORATEDInventors: Joost Ketsman, Luigi Nataloni, Ronny Leontina Marcel Vercauteren
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Patent number: 9988618Abstract: The present invention provides a novel psicose epimerase derived from Flavonifractor plautii and capable of converting fructose to psicose. The novel psicose epimerase according to the present invention possesses an activity producing psicose by epimerizing the carbon-3 position of fructose, and has maximal activity for the conversion of fructose to psicose at a relatively high temperature and a pH less than or equal to neutral, has excellent thermal stability, and can mass-produce psicose from fructose in a high yield for a short amount of time. Therefore, the psicose epimerase according to the present invention is advantageous in the industrial production of psicose, and it is expected that the psicose produced thereby can be usefully utilized in the functional sugar industry and also as materials for health food, medicine, cosmetics, and the like using the psicose.Type: GrantFiled: May 22, 2015Date of Patent: June 5, 2018Assignee: DAESANG CORPORATIONInventors: Tae Gyun Kim, Min Su Kim, Tae Yong Kim, Eun Bum Song, Deok Kun Oh
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Patent number: 9938515Abstract: The present invention relates to: a D-psicose 3-epimerase mutant from Agrobacterium tumefaciens with improved thermal stability; a recombinant vector comprising a gene encoding the mutant; and a microorganism comprising the mutant. In addition, the present invention relates to a method for producing D-psicose by using the epimerase mutant or the microorganism.Type: GrantFiled: April 23, 2014Date of Patent: April 10, 2018Assignee: CJ CHEILJEDANG CORPORATIONInventors: Young Moon Cho, Chang Gyeom Kim, Baek Seok Lee
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Patent number: 9386788Abstract: The present application relates to novel isomaltooligosaccharide (IMO) compositions containing isomaltulose, methods for preparing the same and uses thereof. Specifically, the IMO compositions according to the present application containing isomaltulose, which is a sugar component not contained in currently available isomaltooligosaccharide products, have a quality of sweetness and degree of sweetness differentiated from the existing isomaltooligosaccharide products and thus can be used as sweeteners for more various applications.Type: GrantFiled: May 20, 2014Date of Patent: July 12, 2016Assignee: Corn Products Development, Inc.Inventor: Sang-Wook Ahn
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Patent number: 9217166Abstract: The present invention relates to a D-psicose 3-epimerase variant with improved thermostability by substituting an amino acid at a specific position of an amino acid sequence of a wild type D-psicose 3-epimerase. Further, the present invention provides a recombinant expression vector including a gene of the D-psicose 3-epimerase variant, and a recombinant strain transformed with the recombinant expression vector. Furthermore, the present invention provides an immobilized reactor prepared using the D-psicose 3-epimerase variant or the recombinant strain, and a method of continuously producing D-psicose using the immobilized reactor.Type: GrantFiled: August 21, 2012Date of Patent: December 22, 2015Assignee: CJ CHEILJEDANG CORPORATIONInventors: Yang Hee Kim, Jin Ha Kim, Young Mi Lee, Young Ho Hong, Min Hae Kim, Seong Bo Kim, Seung Won Park, Seung Hyun Oh, Deok Kun Oh, Jin Geun Choi
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Patent number: 9050393Abstract: This invention provides implantable medical devices having at least one moiety attached to the surface capable of catalyzing a reaction in vivo. The implantable medical device has a body such as a stent with a surface adapted to be placed adjacent to a biological tissue or fluid. The body has at least one moiety attached to the surface which is capable of catalyzing a first reaction upon contacting a first substrate in the biological tissue or fluid. The moiety remains attached to the surface after the first reaction and is capable of catalyzing at least a second reaction upon contacting a second substrate. In preferred embodiments the moiety is capable of catalyzing a multitude of reactions as substrates come into contact and then leave the surface of the device.Type: GrantFiled: February 8, 2005Date of Patent: June 9, 2015Inventor: Bruce N. Saffran
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Patent number: 9024110Abstract: This invention discloses the development of a novel platform for recombinant production of bioactive glycoproteins and cancer specific vaccines in plants. Plants and plant cell cultures have been humanized with respect to human mucin-type protein O-glycosylation. A panel of plant cell factories for production of recombinant glycoproteins with designed human O-glycosylation, including an improved cancer vaccine candidate, has been developed. The platform provides basis for i) production of an essentially unlimited array of O-glycosylated human glycoprotein therapeutics, such as human interferon ?2B and podoplanin, and ii) for further engineering of additional cancer specific O-glycans on glycoproteins of therapeutical value. Currently, mammalian cells are required for human O-glycosylation, but plants offer a unique cell platform for engineering O-glycosylation since they do not perform human type O-glycosylation.Type: GrantFiled: March 23, 2011Date of Patent: May 5, 2015Inventors: Zhang Yang, Damian Paul Drew, Emma Adhiambo Arigi, Peter Ulvskov, Steven B. Levery, Eric Bennett, Henrik Clausen, Brent Larsen Petersen
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Publication number: 20150099276Abstract: The present invention relates to the use of nucleic acid molecules coding for a bacterial xylose isomerase (XI), preferably coming from Clostridium phytofermentans, for reaction/metabolization, particularly fermentation, of recombinant microorganisms of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol, by means of xylose fermenting yeasts. The present invention further relates to cells, particularly eukaryotic cells, which are transformed utilizing a nucleic acid expression construct which codes for a xylose isomerase, wherein the expression of the nucleic acid expression construct imparts to the cells the capability to directly isomerize xylose into xylulose. Said cells are preferably utilized for reaction/metabolization, particularly fermentation, of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol.Type: ApplicationFiled: December 16, 2014Publication date: April 9, 2015Inventors: Dawid BRAT, Eckhard BOLES, Marco KELLER, Beate ROTHER
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Patent number: 8986948Abstract: The present invention relates to the use of nucleic acid molecules coding for a bacterial xylose isomerase (XI), preferably coming from Clostridium phytofermentans, for reaction/metabolization, particularly fermentation, of recombinant microorganisms of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol, by means of xylose fermenting yeasts. The present invention further relates to cells, particularly eukaryotic cells, which are transformed utilizing a nucleic acid expression construct which codes for a xylose isomerase, wherein the expression of the nucleic acid expression construct imparts to the cells the capability to directly isomerize xylose into xylulose. Said cells are preferably utilized for reaction/metabolization, particularly fermentation, of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol.Type: GrantFiled: July 1, 2009Date of Patent: March 24, 2015Assignee: Lesaffre et CompagnieInventors: Dawid Brat, Eckhard Boles, Marco Keller, Beate Wiedemann
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Publication number: 20140377798Abstract: A process for the enzymatic regeneration of the redox cofactors NAD+/NADH and NADP+/NADPH in a one-pot reaction, wherein, as a result of at least two further enzymatically catalyzed redox reactions proceeding in the same reaction batch (product-forming reactions), one of the two redox cofactors accumulates in its reduced form and, respectively, the other one in its oxidized form, characterized in that a) in the regeneration reaction which reconverts the reduced cofactor into its original oxidized form, oxygen or a compound of general formula R1C(O)COOH is reduced, and b) in the regeneration reaction which reconverts the oxidized cofactor into its original reduced form, a compound of general formula R2CH(OH)R3 is oxidized and wherein R1, R2 and R3 in the compounds have different meanings.Type: ApplicationFiled: February 6, 2013Publication date: December 25, 2014Inventors: Ortwin Ertl, Nicole Staunig, Marta Sut, Bernd Mayer
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Patent number: 8877437Abstract: Methods of using dyes and associated technology are provided. A dye, such as a monomeric dye or a dimeric dye, may be used in a nucleic acid gel staining application and/or a nucleic acid detection application. A dimeric dye, such as a dimeric dye capable of forming a hairpin-like structure, may be used to stain and/or detect nucleic acids via a release-on-demand mechanism. A dimeric dye having low background fluorescence in the absence of nucleic acids and high fluorescence in the presence of nucleic acids, upon binding therewith, may be used to stain and/or detect nucleic acids. Buffers comprising a weak acid and a salt of the weak acid, such as a lithium salt, are also provided to allow for effective prestaining of nucleic acids.Type: GrantFiled: December 22, 2010Date of Patent: November 4, 2014Assignee: Biotium, Inc.Inventors: Fei Mao, Wai-Yee Leung, Tam Van
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Publication number: 20140302564Abstract: A method for sterilizing microbial cells is provided. According to the method, microbial cells or a culture containing microbial cells are treated with a polyethylene glycol-based nonionic surfactant so that almost all of the microbial cells are sterilized while the enzyme activity expressed in the microbial cells is maintained at a high level. A method for sterilizing microbial cells and a material containing the sterilized microbial cells, in which the microbial cells are sterilized using a polyethylene glycol-based nonionic surfactant, can be used for foods so that the microbial cells are sterilized to be used in food production. Further, a material containing sterilized microbial cells can be used in processes for preparing tagatose, in which Corynebacterium genus microbial cells that produce Galactose and/or Arabinose isomerase are sterilized using a polyethylene glycol-based nonionic surfactant.Type: ApplicationFiled: June 20, 2014Publication date: October 9, 2014Inventors: Jin Ha Kim, Yong Mi Lee, Seong Bo Kim, Taek Beom Kim, Yang Hee Kim, Seung Won Park
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Patent number: 8802393Abstract: The present invention relates to a thermophilic arabinose isomerase and a method of manufacturing tagatose using the same, and more precisely, a gene encoding arabinose isomerase originating from the thermophile Thermotoga neapolitana DSM 5068, a recombinant expression vector containing the gene, a method of preparing a food grade thermophilic arabinose isomerase from the recombinant GRAS (Generally Recognized As Safe) strain transformed with the said expression vector, and a method of preparing tagatose from galactose using the said enzyme.Type: GrantFiled: October 17, 2008Date of Patent: August 12, 2014Assignee: CJ Cheiljedang CorporationInventors: Seong-bo Kim, Young-mi Lee, Seung-won Park, Jung-hoon Kim, Sang-hoon Song, Kang-pyo Lee, Hye-won Kim, Hye-jin Choi
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Patent number: 8790900Abstract: The invention relates to the biotechnological production of isomaltulose and isomaltulose-containing compositions and improved means, therefore particularly microbial cells.Type: GrantFiled: October 23, 2010Date of Patent: July 29, 2014Assignee: Südzucker Aktiengesellschaft Mannheim/OchsenfurtInventors: Stefan Pelzer, Christian Zurek, Thomas Rose, Jürgen Eck, Wolfgang Wach, Michael Klingeberg, Karsten Harms
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Publication number: 20140199732Abstract: The present invention relates to a D-psicose 3-epimerase variant with improved thermostability by substituting an amino acid at a specific position of an amino acid sequence of a wild type D-psicose 3-epimerase. Further, the present invention provides a recombinant expression vector including a gene of the D-psicose 3-epimerase variant, and a recombinant strain transformed with the recombinant expression vector. Furthermore, the present invention provides an immobilized reactor prepared using the D-psicose 3-epimerase variant or the recombinant strain, and a method of continuously producing D-psicose using the immobilized reactor.Type: ApplicationFiled: August 21, 2012Publication date: July 17, 2014Applicant: CJ CHEILJEDANG CORPORATIONInventors: Yang Hee Kim, Jin Ha Kim, Young Mi Lee, Young Ho Hong, Min Hae Kim, Seong Bo Kim, Seung Won Park, Seung Hyun Oh, Deok Kun Oh, Jin Geun Choi
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Publication number: 20140162322Abstract: The invention provides, inter alia, compositions and methods for the biological production of pentose sugars, such as 2-methylerythritol (2-ME), 1-deoxyxylulose (1-DX), and monoacetylated-2-C-methylerythritols, using recombinant cells.Type: ApplicationFiled: February 13, 2014Publication date: June 12, 2014Applicant: DANISCO US INC.Inventors: Joseph C. MCAULIFFE, Rachel E. MUIR
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Patent number: 8691535Abstract: For the biotechnological production of isomaltulose and compositions containing isomaltulose from saccharose the invention provides improved means, particularly a sucrose mutase with improved product specificity as well as microbial cells containing the improved sucrose mutase.Type: GrantFiled: December 18, 2010Date of Patent: April 8, 2014Assignee: Südzucker Aktiengesellschaft Mannheim/OchsenfurtInventors: Wolfgang Aehle, Juergen Eck, Karsten Harms, Michael Klingeberg, Stefan Pelzer, Wolfgang Wach
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Publication number: 20140087429Abstract: The present disclosure relates to an enzyme blend comprising a low pH, thermostable alpha-amylase and a Bacillus licheniformis alpha-amylase. The blend can include at least about 1.0 Liquefon Unit (LU) of the B. licheniformis alpha-amylase for every 5.0 Modified Wohlgemuth Unit (MWU) of the low pH, thermostable alpha-amylase. The enzyme blend described is suitable for starch liquefaction and saccharification, ethanol production, and/or sweetener production, among other things. Also provided herein is a method of processing a starch by liquefying the starch with the low pH, thermostable alpha-amylase and the Bacillus licheniformis alpha-amylase, simultaneously or sequentially.Type: ApplicationFiled: August 29, 2013Publication date: March 27, 2014Applicant: Danisco US Inc.Inventors: Glenn E. Nedwin, Vivek Sharma, Jayarama K. Shetty
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Publication number: 20140011241Abstract: The present invention enables isomerization process flows at lower pH, lower temperature, and in the presence of certain inhibiting compounds by using the xylose isomerase enzyme from the microorganism Fulvimarina pelagi. The xylose isomerase from this marine bacterium not only is very active at the fermentation pH and temperature, it is also tolerant of xylitol and calcium in the amounts generated in biomass fermentation conditions. For the HFCS application, the xylose isomerase from Fulvimarina pelagi is pH compatible with the process and is tolerant of calcium in the amounts found in the HFCS process. The temperature optimum is similar to commercially available amylase/gluco-amylase products.Type: ApplicationFiled: December 21, 2012Publication date: January 9, 2014Inventors: Christopher Charles Beatty, Stephen Jairus Potochnik, Joshua Brandon Kitner
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Publication number: 20140004570Abstract: Provided herein are methods of increasing the efficiency of biomass saccharification. In particular, the methods include ways of avoiding feedback inhibition during the production of useful products.Type: ApplicationFiled: September 3, 2013Publication date: January 2, 2014Applicant: XYLECO, INC.Inventors: Marshall MEDOFF, Thomas Craig MASTERMAN, Michael W. FINN
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Patent number: 8586336Abstract: A novel xylose isomerase nucleotide sequence obtained from a bovine rumen fluid metagenomic library and also provides the amino acid sequence encoded by the nucleotide sequence, and a vector and a transformant containing the nucleotide sequence. When the xylose isomerase is expressed, a host cell is endowed with the capability of converting xylose into xylulose, and the xylulose is further metabolized by the host cell. Therefore, the host cell can take the xylose as a carbon source for growth. The xylose isomerase from a new source is expressed with high activity in Saccharomyces cerevisiae and is a mesophilic enzyme with optimal temperature of 60° C.Type: GrantFiled: June 20, 2011Date of Patent: November 19, 2013Assignee: Shandong UniversityInventors: Xiaoming Bao, Yu Shen, Ruilei Ge
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Publication number: 20130295617Abstract: The present invention provides a method of producing trehalulose, the method comprising: (a) contacting a sucrose solution with a sucrose isomerase enzyme to produce a sucrose-enzyme mixture; and (b) incubating the sucrose-enzyme mixture of (a) at a temperature of less than 10° C. for a period of time sufficient to convert the sucrose in the sucrose-enzyme mixture to a product comprising trehalulose.Type: ApplicationFiled: March 5, 2012Publication date: November 7, 2013Applicant: SYNGENTA PARTICIPATIONS AGInventor: Lauren Shaw
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Patent number: 8399223Abstract: The invention provides a method for treating lung cancer or breast cancer wherein the method comprises administering a pharmaceutically effective dose of arazyme enzyme to a subject with lung cancer or breast cancer. The arazyme enzyme is either a protein comprising the amino acid sequence of SEQ ID NO: 1; and/or a protein encoded by DNA comprising the coding region of the nucleotide sequence of SEQ ID NO: 2.Type: GrantFiled: September 2, 2011Date of Patent: March 19, 2013Assignee: Inssect Biotech Co., Ltd.Inventors: Ho-Yong Park, Kwang-Hee Son, Dong-Ha Shin, Kyu-Shik Jeong
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Patent number: 8389248Abstract: Providing 1- or 6-deoxy products corresponding to all of aldohexoses, ketohexoses and sugar alcohols, as based on Deoxy-Izumoring, as well as a method for systematically producing those products. A method for producing deoxyketohexose and a derivative thereof using a deoxyketohexose isomerase derived from Pseudomonas cichorii ST-24 (FERM BP-2736), comprising epimerizing 1-deoxy D-ketohexose or 6-deoxy D-ketohexose or 1-deoxy L-ketohexose or 6-deoxy L-ketohexose at position 3 to produce the individually corresponding 1-deoxy D-ketohexose or 6-deoxy D-ketohexose or 1-deoxy L-ketohexose or 6-deoxy L-ketohexose as an intended product.Type: GrantFiled: November 20, 2007Date of Patent: March 5, 2013Assignees: National University Corporation Kagawa University, Rare Sugar Production Technical Research Laboratories, LLC., Hayashibara Co., Ltd., Matsutani Chemical Industry Co., Ltd.Inventors: Ken Izumori, Masaaki Tokuda, George Fleet, Yoshio Tsujisaka, Kei Takeshita, Keiji Tsusaki, Kazuhiro Okuma
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Patent number: 8377658Abstract: The present invention relates to a method for producing tagatose using soy oligosaccharide or soluble sugar solution containing the same, more precisely, a method for producing tagatose comprising the following steps; hydrolyzing soy oligosaccharide by using ?-galactosidase selectively; producing tagatose continuously by enzymatic isomerization of galactose obtained from the hydrolysate; separating the produced tagatose by chromatography; and recycling the non-reacted materials.Type: GrantFiled: January 28, 2009Date of Patent: February 19, 2013Assignee: CJ Cheiljedang CorporationInventors: Seong-Bo Kim, Jung-Hoon Kim, Young-Mi Lee, Jin-Ha Kim, Seung-Won Park, Kang-Pyo Lee
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Publication number: 20130029382Abstract: Compositions and methods are provided for treating lignocellulosic material with a dual activity enzyme having xylanase and cellulase activity. The enzyme is stable and active at increased pH and increased temperatures. The present invention therefore provides methods for hydrolyzing lignocellulosic material, especially cellulose and hemicellulose, which are major components of the cell wall of non-woody and woody plants. The methods for hydrolyzing cellulose and hemicellulose can be used on any plant, wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct.Type: ApplicationFiled: January 21, 2011Publication date: January 31, 2013Applicant: SYNGENTA PARTICIPATIONS AGInventors: John C. Steffens, Paul Oeller, Laurent Fourage
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Publication number: 20130029383Abstract: The present invention relates to a method of producing 5-hydroxymethylfurfural by dehydration of fructose and/or glucose and/or mannose.Type: ApplicationFiled: April 7, 2011Publication date: January 31, 2013Applicant: NOVOZYMES A/SInventors: Sven Pedersen, Troels B. Christensen, Astrid Boisen, Vibeke W. Jurgensen, Thomas S. Hansen, Soeren Kegnaes, Anders Riisager, John M. Woodley, Jacob S. Jensen, Wenjing Fu
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Publication number: 20120329098Abstract: The present invention has objects to provide a thermostable cellobiose 2-epimerase, its preparation and uses. The present invention attains the above objects by providing a thermostable cellobiose 2-epimerase, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a process for producing the enzyme, and a process for producing isomerized saccharides using the enzyme.Type: ApplicationFiled: August 21, 2012Publication date: December 27, 2012Applicant: KABUSHIKI KAISHA HAYASHIBARA SEIBUTSU KAGAKU KENKYUJOInventors: Hikaru Watanabe, Masahiro Yagi, Tomoyuki Nishimoto, Hiroto Chaen, Shigeharu Fukuda
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Publication number: 20120315673Abstract: For the biotechnological production of isomaltulose and compositions containing isomaltulose from saccharose the invention provides improved means, particularly a sucrose mutase with improved product specificity as well as microbial cells containing the improved sucrose mutase.Type: ApplicationFiled: December 18, 2010Publication date: December 13, 2012Inventors: Wolfgang Aehle, Juergen Eck, Karsten Harms, Michael Klingeberg, Stefan Pelzer, Wolfgang Wach
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Publication number: 20120270274Abstract: New mannose-6-phasphate isomerase, mutant enzyme thereof, and a method of producing L-ribose using the enzyme are provided, and more specifically, mannose-6-phosphate isomerase, mutant enzyme thereof, recombinant expression vectors including relevant genes, microorganisms transformed with the vectors, a method of producing mannose-6-phosphate isomerase or mutant thereof in bulk using them, and a high yield method of producing L-ribose using the mannose-6-isomerase or the mutant thereof, are provided.Type: ApplicationFiled: October 22, 2010Publication date: October 25, 2012Applicant: Konkuk University Industrial Cooperation Corp.Inventors: Deok Kun Oh, Soo Jin Yeom
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Publication number: 20120252073Abstract: A method of decomposing stubble, which is crop residue left on the ground, and a composition for treating the stubble are disclosed. The method comprises treating the stubble with the composition comprising a) polysaccharide-degrading enzyme; b) wetting agent; and, optionally, c) water and allowing the treated stubble to remain on the ground and decompose.Type: ApplicationFiled: March 28, 2012Publication date: October 4, 2012Applicant: RHODIA OPERATIONSInventors: Jean-Christophe Castaing, Gary Groves, Ashwin Rao, Thomas E. Ruch
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Publication number: 20120225452Abstract: A novel xylose isomerase nucleotide sequence obtained from a bovine rumen fluid metagenomic library and also provides the amino acid sequence encoded by the nucleotide sequence, and a vector and a transformant containing the nucleotide sequence. When the xylose isomerase is expressed, a host cell is endowed with the capability of converting xylose into xylulose, and the xylulose is further metabolized by the host cell. Therefore, the host cell can take the xylose as a carbon source for growth. The xylose isomerase from a new source is expressed with high activity in Saccharomyces cerevisiae and is a mesophilic enzyme with optimal temperature of 60° C.Type: ApplicationFiled: June 20, 2011Publication date: September 6, 2012Applicant: SHAN DONG UNIVERSITYInventors: Xiaoming Bao, Yu Shen, Ruilei GE
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Publication number: 20120225458Abstract: The invention relates to the biotechnological production of isomaltulose and isomaltulose-containing compositions and improved means, therefore particularly microbial cells.Type: ApplicationFiled: October 23, 2010Publication date: September 6, 2012Applicant: SUDZUCKER AKTIENGESELLSCHAFT MANNHEIM/OCHSENFURTInventors: Stefan Pelzer, Christian Zurek, Thomas Rose, Jürgen Eck, Wolfgang Wach, Michael Klingeberg, Karsten Harms
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Patent number: 8227232Abstract: Object: To provide a thermostable L-ribose isomerase. Means for Resolution: The thermostable L-ribose isomerase with MW. 32,000 (by SDS-PAGE), optimal temperature of 45° C., optimal pH of pH 9.0 (glycine-NaOH buffer), and stable physicochemical properties such as temperature stability up to 45° C. during thermal treatment at pH 9.0 for 10 minutes, and with an action to isomerize L-ribose to generate L-ribulose or of inversely to isomerize L-ribulose to generate L-ribose.Type: GrantFiled: March 26, 2007Date of Patent: July 24, 2012Assignees: National University Corporation Kagawa Univeristy, Rare Sugar Production Technical Research Laboratories, LLC., Matsutani Chemical Industry Co., Ltd.Inventors: Ken Izumori, Kenji Morimoto, Goro Takata, Masaaki Tokuda, Yoshio Tsujisaka, Kei Takeshita, Keiji Tsusaki, Kazuhiro Okuma
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Publication number: 20120115191Abstract: There is provided a new strain, that is Aspergillus fumigatus, MTCC 5453, and its use in the production of fructo-oligosaccharide. The invention also provides an efficient, rapid and method for increased production of fructo-oligosaccharide by using the strain Aspergillus fumigatus MTCC 5453.Type: ApplicationFiled: February 28, 2010Publication date: May 10, 2012Inventors: Samir S. Somaiya, Sangeeta Srivastava, Martin Annette, Amol Dive
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Publication number: 20120040407Abstract: The present invention has objects to provide a thermostable cellobiose 2-epimerase, its preparation and uses. The present invention attains the above objects by providing a thermostable cellobiose 2-epimerase, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a process for producing the enzyme, and a process for producing isomerized saccharides using the enzyme.Type: ApplicationFiled: January 25, 2010Publication date: February 16, 2012Applicant: Kabushiki Kaisha Hayashibara Seibutsu Kagaku KenkyujoInventors: Hikaru Watanabe, Masahiro Yagi, Tomoyuki Nishimoto, Hiroto Chaen, Shigeharu Fukuda
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Publication number: 20110281817Abstract: Methods for producing hyaluronic acid are described, including altering the activity in Streptococcus cells of one or more enzymes and/or altering the amount of available substrates or substrate precursors.Type: ApplicationFiled: August 29, 2008Publication date: November 17, 2011Applicants: SUGAR INDUSTRY INNOVATION PTY. LTD., THE UNIVERSITY OF QUEENSLANDInventors: Lars Keld Nielsen, Wendy Chen, Esteban Stefane Marcellin Saldana
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Publication number: 20110269180Abstract: The present invention relates to the use of nucleic acid molecules coding for a bacterial xylose isomerase (XI), preferably coming from Clostridium phytofermentans, for reaction/metabolization, particularly fermentation, of recombinant microorganisms of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol, by means of xylose fermenting yeasts. The present invention further relates to cells, particularly eukaryotic cells, which are transformed utilizing a nucleic acid expression construct which codes for a xylose isomerase, wherein the expression of the nucleic acid expression construct imparts to the cells the capability to directly isomerize xylose into xylulose. Said cells are preferably utilized for reaction/metabolization, particularly fermentation, of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol.Type: ApplicationFiled: July 1, 2009Publication date: November 3, 2011Inventors: Dawid Brat, Eckhard Boles, Marco Keller, Beate Wiedemann
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Publication number: 20110244525Abstract: The present invention relates to a transformed microorganism capable of (a) a higher xylose isomerase activity than the equivalent microorganism prior to transformation; and/or (b) a higher growth rate in or on a growth medium comprising xylose than the equivalent microorganism prior to transformation; and/or (c) a faster metabolism of xylose than the equivalent microorganism prior to transformation; and/or (d) a higher production of ethanol when grown anaerobically on xylose as the carbon source than the equivalent microorganism prior to transformation.Type: ApplicationFiled: December 10, 2009Publication date: October 6, 2011Applicant: TERRANOL A/SInventors: Birgitte Rönnow, Thomas Hvid Andersen, Ole Sibbesen
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Patent number: 8030035Abstract: Provided is a method of producing D-psicose using a D-psicose epimerase derived from Agrobacterium tumefaciens. Provided are a protein having an amino acid sequence of SEQ ID NO:1 and having a psicose 3-epimerase activity, a gene encoding the protein, a recombinant expression vector containing the gene, and a method of producing D-psicose by reacting the protein produced on a mass scale with D-fructose. The method of producing D-psicose is an environmentally friendly method using a new enzyme, in which an inexpensive substrate is used, and the activity of the enzyme can be retained for a prolonged time period. Thus, the method can be efficiently used for the mass production of D-psicose.Type: GrantFiled: May 30, 2006Date of Patent: October 4, 2011Assignee: CJ Cheiljedang Corp.Inventors: Deok-Kun Oh, Hye-Jung Kim, Yong-Joo Lee, Sang-Hoon Song, Seung-Won Park, Jung-Hoon Kim, Seong-Bo Kim
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Patent number: 8008058Abstract: An object of the present invention is to provide a novel ketose 3-epimerase, a process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, and a process for producing a ketose by using the enzyme. The present invention solves the above objects by providing a ketose 3-epimerase which is obtainable from a microorganism of the genus Rhizobium, a process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, and a process for converting D- or L-ketohexose into corresponding D- or L-ketohexose by epimerizing the hydroxyl group at the C-3 position of the D- or L-ketohexose; and D- or L-ketopentose into corresponding D- or L-ketopentose by epimerizing the hydroxyl group at the C-3 position of the D- or L-ketopentose; by using the enzyme.Type: GrantFiled: November 6, 2006Date of Patent: August 30, 2011Assignee: Kabushiki Kaisha Hayashibara Seibutsu Kagaku KenkyujoInventors: Kazuhiko Maruta, Kozo Yamamoto, Tomoyuki Nishimoto, Hiroto Chaen, Tetsuya Nakada
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Publication number: 20110207181Abstract: The invention is directed to novel enzymes that convert sucrose to isomaltulose. More particularly, the present invention discloses novel sucrose isomerases, polynucleotides encoding these sucrose isomerases, methods for isolating such polynucleotides and nucleic acid constructs that express these polynucleotides. Also disclosed are cells, including transformed bacterial or plant cells, and differentiated plants comprising cells, which contain these sucrose isomerase-encoding polynucleotides, as well as extracts thereof. Methods of producing isomaltulose are also disclosed which use the polypeptides, polynucleotides, cells, cell extracts and plants of the invention.Type: ApplicationFiled: February 8, 2011Publication date: August 25, 2011Applicant: The University of Queensland, St. LuciaInventors: Robert George BIRCH, Luguang WU
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Patent number: 7935807Abstract: The invention relates to a novel polynucleotide exhibiting a promoting transcription activity, polynucleotide-containing vectors and to the use thereof for the transcription of interesting sequences such as the production of non-cap RNA virus. Said invention also relates to host cells preferably of avian origin, containing a polynucleotide or the inventive vector.Type: GrantFiled: February 27, 2004Date of Patent: May 3, 2011Assignees: Institut Pasteur, Centre National de la Recherche Scientifique (SNRS), Universite Paris 7-Denis DiderotInventors: Nadia Naffakh, Pascale Massin, Sylvie Van Der Werf
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Patent number: 7923222Abstract: This invention provides a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases with improved catalytic activity and thermostability obtained by using recombinant techniques. These recombinant glucose isomerases comprise amino acid variation including phenylalanine (Phe) at position 139, alanine (Ala) at position 182, serine (Ser) at position 187, and glutamine (Gin) at position 299, and carry at least one additional mutated amino acid at position 87, position 217, position 260 or position 276, and possess a higher catalytic activity than that of the wild-type when using D-glucose as substrate. These recombinant glucose isomerases can be used for direct production of high fructose corn syrup containing 55% [wt] or higher concentration of fructose.Type: GrantFiled: March 15, 2010Date of Patent: April 12, 2011Assignee: Geneharbor (HK) Technologies Ltd.Inventors: Jun Wang, Caike Jin, Rongzhao Fu, Dong Shen
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Patent number: 7919300Abstract: This invention describes a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases having improved catalytic activity and thermostability. The recombinant glucose isomerases can be used for direct production of fructose syrup containing 55 wt % or higher concentration of fructose, or used for the production of fructose syrup containing less than 55 wt % fructose.Type: GrantFiled: July 28, 2004Date of Patent: April 5, 2011Inventors: Jun Wang, Rongzhao Fu, Dong Shen, Caike Jin, Zhangming Liu, Junming Chen
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Publication number: 20110065152Abstract: The present invention deals with an improved process for the production of high yield of pure Galactooligosaccharides using microbial whole cells in a reactor with cross flow hollow fiber microfiltration system. The process is economical as cell biomass is used repeatedly and eliminated the need to carry out downstream processing for the removal of mono and disacchacrides from the final product.Type: ApplicationFiled: March 12, 2009Publication date: March 17, 2011Inventors: Uday Kashinath Avalakki, Palamalai Maheswaran, Rengarajan Saravanan