Produced By The Action Of A Carbohydrase (e.g., Maltose By The Action Of Alpha Amylase On Starch, Etc.) Patents (Class 435/99)
  • Patent number: 11180786
    Abstract: The present invention provides an improved process of treating crop kernels to provide a starch product of high quality suitable for conversion of starch into mono- and oligosaccharides, ethanol, sweeteners. The present invention also provides polypeptides having GH10 xylanase activity, polypeptides having GH62 arabinofuranosidase activity and the uses thereof.
    Type: Grant
    Filed: November 24, 2017
    Date of Patent: November 23, 2021
    Assignee: Novozymes A/S
    Inventors: Yi Cao, Yu Zhang, Bernardo Vidal, Jr., Randall Scott Deinhammer, Mary Ann Stringer, Ye Liu, Purna Venkatesh, Cui Liu
  • Patent number: 11174501
    Abstract: A method of enzyme-based processing of plant-based materials includes steps of providing a bulk amount of a plant-based material having a substantial amount of the cell walls in a physically-intact condition; providing an enzyme broth having an enzyme capable of breaking down the cell walls in the physically-intact condition; combining the bulk amount of the plant-based material with the enzyme broth; allowing the enzyme capable of breaking down the cell walls in the physically-intact condition to break down at least a portion of the cell walls in the physically-intact condition to thereby produce intact oil bodies, hydrolyzed carbohydrates, and intact protein bodies; collecting a first product including the intact oil bodies; collecting a second product including the hydrolyzed carbohydrates; and collecting a third product including the intact protein bodies.
    Type: Grant
    Filed: December 16, 2019
    Date of Patent: November 16, 2021
    Assignee: The University of Akron
    Inventor: Lu-Kwang Ju
  • Patent number: 11142668
    Abstract: Processes disclosed are capable of converting biomass into high-crystallinity, hydrophobic cellulose. In some variations, the process includes fractionating biomass with an acid (such as sulfur dioxide), a solvent (such as ethanol), and water, to generate cellulose-rich solids and a liquid containing hemicellulose and lignin; and depositing lignin onto cellulose fibers to produce lignin-coated cellulose materials (such as dissolving pulp). The crystallinity of the cellulose material may be 80% or higher, translating into good reinforcing properties for composites. Optionally, sugars derived from amorphous cellulose and hemicellulose may be separately fermented, such as to monomers for various polymers. These polymers may be combined with the hydrophobic cellulose to form completely renewable composites.
    Type: Grant
    Filed: October 25, 2019
    Date of Patent: October 12, 2021
    Assignee: GranBio Intellectual Property Holdings, LLC
    Inventors: Kimberly Nelson, Theodora Retsina, Vesa Pylkkanen, Ryan O'Connor
  • Patent number: 10995023
    Abstract: The water treatment device is an AOP (advanced oxidization process) apparatus that provides effective sanitation of pool water. The device includes couplings that can adapt to fit various standard pool equipment. Inside the reaction chamber of the main body, there are a nozzle and a metal catalyst. The nozzle creates water jet impinging the metal catalyst, which subsequently creates bubbles. Being entrained in the water, the bubbles substantially collapse and create high temperature and pressure changes. These changes cause water to go through chemical dissociation, thus creating hydroxyl radicals, a highly reactive oxidizer. In a fast rate, the hydroxyls attach to and destroy organic contaminants in the water. The metal catalyst remains chemically unchanged. The apparatus has a long service life and substantially reduces the use of chemicals in a pool.
    Type: Grant
    Filed: August 13, 2018
    Date of Patent: May 4, 2021
    Assignee: POLAR VORTEX, LLC
    Inventor: Gary Powell
  • Patent number: 10961324
    Abstract: A process is disclosed for converting cellulose to nanocellulose. The process comprises dissolving a cellulose feedstock in an inorganic molten salt solvent medium. The solvent medium is substantially proton free. The solvent medium may contain a proton scavenger. The process is carried out at relatively low temperatures, and requires little input of mechanical energy.
    Type: Grant
    Filed: September 29, 2016
    Date of Patent: March 30, 2021
    Assignee: BIOECON INTERNATIONAL HOLDING N.V.
    Inventors: Paul O'Connor, Igor Babich, Jacobus Johannes Leonardus Heinerman, Lixian Xu, Johan Van Den Bergh
  • Patent number: 10934551
    Abstract: Fungi that are genetically inactivated for the mstC gene (or a homolog thereof) are provided, which can also be genetically modified to increase production of heterologous proteins from a glucoamylase promoter. Methods of using these fungi, for example to degrade a biomass, are also provided.
    Type: Grant
    Filed: October 17, 2018
    Date of Patent: March 2, 2021
    Assignees: Battelle Memorial Institute, Natl Tech & Engineering Solutions of Sandia, LLC
    Inventors: Scott E. Baker, Jon K. Magnuson, Morgann C. Reilly, Joonhoon Kim, John Gladden, Jed J. Lynn
  • Patent number: 10907183
    Abstract: The invention relates to a process for the preparation of a fermentation product from lignocellulosic material, comprising the following steps: a) optionally, pre-treatment of the lignocellulosic material, b) optionally, washing of the optionally pretreated lignocellulosic material, c) enzymatic hydrolysis of the optionally washed and/or optionally pretreated lignocellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises LPMO, and optionally purifying the hydrolysed lignocellulosic material, d) fermentation of the hydrolysed lignocellulosic material to produce a fermentation product, and e) optionally, recovery of a fermentation product, wherein oxygen is consumed in amounts corresponding to between 20 and 5000 mmol molecular oxygen per kg glucan present in the lignocellulosic material, the oxygen is added after the pretreatment and before and/or during the enzymatic hydrolysis of the lignocellulosic material, preferably in an
    Type: Grant
    Filed: July 31, 2019
    Date of Patent: February 2, 2021
    Assignee: DSM IP ASSETS B.V.
    Inventors: Bertus Noordam, Michael Petrus Jozef Berkhout
  • Patent number: 10883098
    Abstract: In one aspect, the invention is directed to polypeptides having an amylase and/or glucoamylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. In one aspect, the polypeptides of the invention can be used as amylases, for example, alpha amylases, to catalyze the hydrolysis of polysaccharide, oligosaccharide or starch into sugars. In one aspect, the invention provides delayed release compositions comprising an desired ingredient coated by a latex polymer coating. In alternative embodiments, enzymes are used to make biofuels, e.g., ethanol, butanol, propanol, or a gasoline-ethanol mix, including a bioethanol, biopropanol, biobutanol, or a biodiesel, or for any form of fuel or biomass processing.
    Type: Grant
    Filed: October 11, 2018
    Date of Patent: January 5, 2021
    Assignees: BASF Enzymes LLC, Syngenta Participations AG
    Inventors: Malgorzata Slupska, Geoff Hazlewood, Cathy Chang, Peter Luginbuhl, Ellen Burke, Michelle Cayouette, Uvini Gunawardena, Majid Ghassemian, Aron Silverstone, Yan Zhang
  • Patent number: 10876141
    Abstract: The present invention is directed to a novel and advantageous process for the hydrolysis of biomass which enables a thorough hydrolyzation also of recalcitrant biomass such as sugar cane straw and sugar cane bagasse.
    Type: Grant
    Filed: May 12, 2016
    Date of Patent: December 29, 2020
    Assignee: Clariant International Ltd.
    Inventors: Michael Zavrel, Markus Zehe, Joerg Bartuch, Marcus Verhuelsdonk
  • Patent number: 10858685
    Abstract: Provided is a method for industrially mass-producing a modified xylopolysaccharide from biomass. The biomass used as a raw material includes xylan in plant cell walls, having at least one kind of substituents selected from acetyl, feruloyl arabinofuranosyl and coumaroyl arabinofuranosyl groups in the side chains of xylan. A passage controlling mechanism is internally arranged to generate plug-flow onto a slurry containing the biomass at a solid content in 10 mass % to 30 mass %. The hydrothermal treatment is performed under the controlled conditions: at a temperature of 160° C. or more, at a pressure equal to or higher than the saturated water vapor pressure at said temperature, and with a reaction severity R0 ranging from 3000 to 7000. A modified xylopolysaccharide is obtained as preserving the substituents in the side chains of xylan by performing a continuous hydrothermal treatment in a cylindrical plug-flow reactor.
    Type: Grant
    Filed: March 28, 2016
    Date of Patent: December 8, 2020
    Assignees: B Food Science Co., Ltd., Kimura Chemical Plants Co., Ltd.
    Inventors: Satoshi Makishima, Hirofumi Ikeda, Yousuke Yamakawa, Yoshihiko Amano, Nobuaki Sato, Tsutomu Arai
  • Patent number: 10793887
    Abstract: The invention relates to methods of saccharifying a cellulosic material comprising subjecting the cellulosic material to a laccase and a cellulolytic enzyme composition comprising a GH61 polypeptide in the presence of dissolved oxygen at a concentration in the range of 0.5-90% of the saturation level. The invention also related to methods of producing desired fermentation products, such as ethanol, using a method including a saccharification step of the invention.
    Type: Grant
    Filed: August 21, 2015
    Date of Patent: October 6, 2020
    Assignee: Novozymes A/S
    Inventors: Hui Xu, Jesper Frickmann, Katja Salomon Johansen
  • Patent number: 10793886
    Abstract: The invention is in the field of enzymology. More in particular, it provides a method for the isomerization of xylose into xylulose wherein the xylose is derived from lignocellulosic material.
    Type: Grant
    Filed: June 20, 2017
    Date of Patent: October 6, 2020
    Assignee: METGEN OY
    Inventors: Klara Birikh, Anu Minna Maaret Suonpää, Matti Wilhelm Heikkilä
  • Patent number: 10703770
    Abstract: The present invention provides for a method for dissolving and/or depolymerizing lignin comprising: (a) providing a composition comprising lignin, (b) contacting the composition with a strong hydrogen donor, such as a polyol, to form a first solution, (c) incubating the first solution at a temperature equal to or less than 100° C., whereby at least 20% by weight of the lignin is dissolved, (d) optionally introducing an oxidation agent to the first solution to form a second solution, wherein the temperature of the second solution is equal to or less than 100° C., whereby lignin is depolymerized, and (e) optionally introducing an anti-solvent to the second solution to precipitate the depolymerized lignin.
    Type: Grant
    Filed: June 6, 2017
    Date of Patent: July 7, 2020
    Assignee: National Technology & Engineering Solutions of Sandia, LLC
    Inventors: Jian Sun, Tanmoy Dutta, Parthasarathi Ramakrishnan, Blake A. Simmons, Seema Singh
  • Patent number: 10676769
    Abstract: The present invention relates to cellobiohydrolase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Grant
    Filed: September 8, 2015
    Date of Patent: June 9, 2020
    Assignee: Novozymes A/S
    Inventors: Peter Westh, Kim Borch, Trine Soerensen, Michael Windahl, Brett McBrayer
  • Patent number: 10612062
    Abstract: A method of efficiently producing saccharides having glucose as the major component by inexpensively suppressing the non-productive adsorption of the enzyme to lignin is provided. The method of producing saccharides includes: a first step of preparing a water-soluble protein by adding at least any one of an animal protein and a vegetable protein to an aqueous sodium hydroxide solution or an aqueous calcium hydroxide solution to react with each other; a second step of adding the water-soluble protein to a slurry including a biomass; and a third step of producing saccharides having glucose as a major component by adding a degrading enzyme to the slurry for at least any one of a cellulose or a hemicellulose included in the biomass to be degraded by the degrading enzyme simultaneously with addition of the water-soluble protein to the slurry or after addition of the water-soluble protein.
    Type: Grant
    Filed: January 30, 2015
    Date of Patent: April 7, 2020
    Assignee: JGC Corporation
    Inventor: Daisuke Taneda
  • Patent number: 10557153
    Abstract: The present invention describes a method for the treatment of lignocellulosic material which method comprises contacting said lignocellulosic material with a composition comprising two or more enzyme activities, said enzyme activities being cellulase and/or hemicellulase activities, wherein the pH during the treatment is about 4.5 or lower, and the treatment is carried out at a dry matter content of 15% or more.
    Type: Grant
    Filed: August 4, 2009
    Date of Patent: February 11, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: Wilhelmus Theodorus Antonius Maria De Laat, Manoj Kumar, Margot Elisabeth Francoise Schooneveld-Bergmans
  • Patent number: 10544440
    Abstract: The present disclosure relates to compositions and methods useful for the production of heterologous proteins in filamentous fungal cells.
    Type: Grant
    Filed: March 11, 2019
    Date of Patent: January 28, 2020
    Assignee: GLYKOS FINLAND OY
    Inventors: Christopher Landowski, Anne Huuskonen, Ann Westerholm-Parvinen, Markku Saloheimo, Anne Kanerva, Jukka Hiltunen
  • Patent number: 10533202
    Abstract: The current disclosure provides a process for enzymatically converting a saccharide into tagatose. The invention also relates to a process for preparing tagatose where the process involves converting fructose 6-phosphate (F6P) to tagatose 6-phosphate (T6P), catalyzed by an epimerase, and converting the T6P to tagatose, catalyzed by a phosphatase.
    Type: Grant
    Filed: September 28, 2018
    Date of Patent: January 14, 2020
    Assignee: BONUMOSE LLC
    Inventor: Daniel Joseph Wichelecki
  • Patent number: 10519432
    Abstract: The present invention relates to xylanase variants, polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; compositions comprising the xylanase variants and methods of using the variants.
    Type: Grant
    Filed: December 19, 2018
    Date of Patent: December 31, 2019
    Assignee: Novozymes A/S
    Inventors: Steffen Danielsen, Peter Kamp Hansen, Frank Winther Rasmussen, Roland Alexander Pache
  • Patent number: 10465210
    Abstract: Provided are a recombinant protein, a gene encoding the recombinant protein, a recombinant microorganism including the gene, and a method of degrading a macromolecular substance using the recombinant microorganism or the recombinant protein.
    Type: Grant
    Filed: October 4, 2017
    Date of Patent: November 5, 2019
    Assignee: SAMSUNG ELECTRONICS CO., LTD.
    Inventors: Yukyung Jung, Taeyong Kim, Seunghoon Song, Jiae Yun, Jinhwan Park, SunJung Byun
  • Patent number: 10428334
    Abstract: A heterologous gene cassette useful for creating Streptomyces species with enhanced capability of growing on a cellulosic polysaccharide substrate, wherein the cassette comprises at least two members of the following categories: a) a GH6 gene, b) an AA10 gene, c) a GH48 gene, d) a GH5 gene and e) either (i) a GH9 gene, (ii) a GH9 gene and a GH12 gene, or (iii) a GH12 gene is disclosed.
    Type: Grant
    Filed: April 4, 2017
    Date of Patent: October 1, 2019
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Brian Grant Fox, Robert Joseph Stankey, Cameron Robert Currie, Emily Beebe
  • Patent number: 10428322
    Abstract: The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast Saccharomyces cerevisiae. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.
    Type: Grant
    Filed: January 10, 2019
    Date of Patent: October 1, 2019
    Assignee: Lallemand Hungary Liquidity Management LLC
    Inventors: Elena E. Brevnova, Vineet Rajgarhia, Mark Mellon, Anne Warner, John McBride, Chhayal Gandhi, Erin Wiswall
  • Patent number: 10392742
    Abstract: The present disclosure relates to a biofinishing system comprising a combination of cellulases, in particular a biofinishing system comprising a combination of GH45 cellulases. The present disclosure further relates to a process for treating a cellulose-containing textile comprising biofinishing the cellulose-containing textile with a combination of GH45 cellulases.
    Type: Grant
    Filed: June 24, 2016
    Date of Patent: August 27, 2019
    Assignee: Novozymes A/S
    Inventors: Weijian Lai, Wenqi Huang, Wei Wang
  • Patent number: 10337144
    Abstract: Described is a method for processing cellulose-containing biomass with sulfuric acid and certain additives, especially for the pretreatment of cellulose-containing biomass prior to saccharification.
    Type: Grant
    Filed: February 18, 2016
    Date of Patent: July 2, 2019
    Assignee: BASF SE
    Inventors: Frank Rittig, Stefan Koch, Alois Kindler, Michael Koch, Ferdinand Leifeld, Vaidotas Navickas, Markus Gruen
  • Patent number: 10316466
    Abstract: It is disclosed a process to transfer a ligno-cellulosic feedstock from a low pressure zone to a high pressure zone. A slurry the ligno-cellulosic feedstock is first provided, which is then subjected to a multi-step liquid removal process comprising draining by gravity at least a portion of the free liquid; removing a first portion of the capillary liquid in a low pressure zone by continuously compressing a stream of the ligno-cellulosic feedstock to reach a moisture content in a first target range; removing a second portion of the capillary liquid by shearing and compressing the ligno-cellulosic stream in a plug forming device, to form a ligno-cellulosic feedstock plug having a moisture content in a second target range at an outlet of the plug forming device connected to the high pressure zone.
    Type: Grant
    Filed: January 28, 2016
    Date of Patent: June 11, 2019
    Assignee: Versalis S.p.A.
    Inventors: Guido Ghisolfi, Ettore Bonaldi
  • Patent number: 10316106
    Abstract: Described is a method for processing cellulose-containing biomass with sulfuric acid and certain additives, especially for the pretreatment of cellulose-containing biomass prior to saccharification.
    Type: Grant
    Filed: February 18, 2016
    Date of Patent: June 11, 2019
    Assignee: BASF SE
    Inventors: Frank Rittig, Stefan Koch, Alois Kindler, Michael Koch, Ferdinand Leifeld, Vaidotas Navickas, Markus Gruen
  • Patent number: 10294466
    Abstract: The present disclosure relates to polypeptides having alpha-glucosidase activity isolated, derived or derivable from Rasamsonia or engineered polypeptides having alpha-glucosidase activity isolated, derived or derivable from Rasamsonia homologs. The present disclosure also pertains to polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, host cells and mutant cells comprising the polynucleotides. The disclosure further pertains to compositions comprising such polypeptides, methods of producing the polypeptides and compositions, as well as methods for using such polypeptides and compositions for industrial applications.
    Type: Grant
    Filed: February 25, 2016
    Date of Patent: May 21, 2019
    Assignee: DANISCO US INC.
    Inventors: Zheyong Yu, Zhenghong Zhang, Jing Ge, Zhen Qian, Guoqing Liu, Zhiyong Xie, Zhongmei Tang
  • Patent number: 10266856
    Abstract: A method for preparing a fermentation product including lactic acid, the method including: a) treating lignocellulosic material with caustic magnesium salt in the presence of water to provide treated aqueous lignocellulosic material; b) saccharifying the treated aqueous lignocellulosic material in the presence of a hydrolytic enzyme to provide a saccharified aqueous lignocellulosic material comprising fermentable carbohydrate and a solid lignocellulosic fraction; c) simultaneously with step b), fermenting the saccharified aqueous lignocellulosic material in the presence of both a lactic acid forming microorganism and caustic magnesium salt to provide an aqueous fermentation broth comprising magnesium lactate and a solid lignocellulosic fraction; d) recovering magnesium lactate from the broth, wherein the saccharification and the fermentation are carried out simultaneously.
    Type: Grant
    Filed: July 28, 2015
    Date of Patent: April 23, 2019
    Assignee: PURAC BIOCHEM BV
    Inventors: Peter Johannes Marie Baets, David Sanchez Garcia, Willem Jacob Groot, André Banier De Haan
  • Patent number: 10259841
    Abstract: A method for reducing the level of DNA in a fermentation broth is disclosed where the method includes a heating step where the broth is heated to a temperature of at least 70° C.
    Type: Grant
    Filed: April 30, 2015
    Date of Patent: April 16, 2019
    Assignee: NOVOZYMES A/S
    Inventors: Cedric Hobel, Heidi Winterberg Andersen, Peter Frode Pind, Cecilia Jansson Kepka
  • Patent number: 10240006
    Abstract: Disclosed are methods of functionalizing lignin using reactive extraction. The invention also generally relates to functionalized lignin. An exemplary method for producing functionalized lignin is disclosed, which comprises: providing a residue comprising lignin and cellulose; subjecting the residue to a liquid comprising an organic compound, thereby forming a first mixture; wherein the subjecting is performed at a first temperature of about 100° C. or less; and wherein the first mixture comprises: a first liquid fraction comprising solubilized functionalized lignin, wherein the solubilized functionalized lignin is functionalized with the organic compound; and a first solid fraction comprising cellulose.
    Type: Grant
    Filed: May 1, 2015
    Date of Patent: March 26, 2019
    Assignee: Renmatix, Inc.
    Inventors: Mikhail Y. Balakshin, Ewellyn A. Capanema, Manuk Colakyan, Francis Lipiecki
  • Patent number: 10221406
    Abstract: The present invention relates to cellobiohydrolase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Grant
    Filed: March 7, 2014
    Date of Patent: March 5, 2019
    Assignees: Novozymes, Inc., Novozymes A/S
    Inventors: Kim Borch, Kenneth Jensen, Kristian Krogh, Brett McBrayer, Peter Westh, Jeppe Kari, Johan Olsen, Trine Sorensen, Michael Windahl, Hui Xu
  • Patent number: 10190107
    Abstract: The present invention has a purpose of providing a technique for increasing the heat resistance of a ?-galactosidase. According to the present invention, in a reference ?-galactosidase amino acid sequence which shows a 90% or more identity to the amino acid sequence of SEQ ID NO: 4, proline is substituted for one or more amino acids selected from the group consisting of the following amino acids: (1) an amino acid corresponding to lysine at position 166 of the amino acid sequence of SEQ ID NO: 4, (2) an amino acid corresponding to glycine at position 307 of the amino acid sequence of SEQ ID NO: 4, and (3) an amino acid corresponding to alanine at position 833 of the amino acid sequence of SEQ ID NO: 4.
    Type: Grant
    Filed: August 13, 2015
    Date of Patent: January 29, 2019
    Assignee: Amano Enzyme Inc.
    Inventors: Kazuhiko Ishikawa, Satoru Ishihara, Shotaro Yamaguchi
  • Patent number: 10150843
    Abstract: Methods and systems are provided for improved liquefaction of a lignocellulosic biomass. The methods include reacting a lignocellulosic biomass slurry at mild conditions for a short period to produce a first reaction mixture having a reduced viscosity as compared to the lignocellulosic biomass slurry and that is substantially free of levulinic acid and hydroxymethylfurfural. The methods further include separating the first reaction mixture into a solid fraction and a liquid fraction, the solid fraction having a majority of lignin from the lignocellulosic biomass slurry and the liquid fraction having a majority of hemicellulose and greater than about 30% by weight of cellulose from the lignocellulosic biomass slurry. The method advantageously prevents or eliminates the formation of tar and/or char.
    Type: Grant
    Filed: March 16, 2016
    Date of Patent: December 11, 2018
    Assignee: Georgia-Pacific LLC
    Inventors: Michael E. Carroll, Johannes A. Kroon, Rudy F. M. J. Parton, Subrata Sen, Pierre L. Woestenborghs
  • Patent number: 10138506
    Abstract: The current disclosure provides a process for enzymatically converting a saccharide into tagatose. The invention also relates to a process for preparing tagatose where the process involves converting fructose 6-phosphate (F6P) to tagatose 6-phosphate (T6P), catalyzed by an epimerase, and converting the T6P to tagatose, catalyzed by a phosphatase.
    Type: Grant
    Filed: September 30, 2016
    Date of Patent: November 27, 2018
    Assignee: BONUMOSE LLC
    Inventor: Daniel Joseph Wichelecki
  • Patent number: 10131923
    Abstract: The invention relates to a process for the preparation of a fermentation product from ligno-cellulosic material, comprising the following steps: a) optionally pre-treatment of the ligno-cellulosic material; b) optionally washing of the optionally pre-treated ligno-cellulosic material; c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61; d) whereby less than 7.5 mg enzyme composition/g glucan (on dry matter and enzyme as protein) or less than 3.0 mg enzyme composition/g feedstock (on dry matter and enzyme as protein) is used; and e) fermentation of the hydrolyzed ligno-cellulosic material to produce a fermentation product; and f) optionally recovery of a fermentation product; wherein before and/or during the enzymatic hydrolysis oxygen is added to the ligno-cellulosic material.
    Type: Grant
    Filed: April 19, 2018
    Date of Patent: November 20, 2018
    Assignee: DSM IP ASSETS B.V.
    Inventors: Bertus Noordam, Michael Petrus Jozef Berkhout
  • Patent number: 10081802
    Abstract: Disclosed are glycosyl hydrolase enzyme variants, particularly variants of certain oxidoreductases of glycosyl hydrolase family 61. Nucleic acids encoding the glycosyl hydrolyase variants, compositions including the glycosyl hydrolase variants, methods of producing the variants, and methods of using the variants are also described.
    Type: Grant
    Filed: July 24, 2014
    Date of Patent: September 25, 2018
    Assignee: DANISCO US INC.
    Inventors: Maria Foukaraki, Ronaldus Wilhelmus Hommes, Thijs Kaper, Bradley R. Kelemen, Slavko Kralj, Suzanne E. Lantz, Igor Nikolaev, Wilhelmus Van Der Kley, Johannes Franciscus Thomas Van Lieshout, Sander Van Stigt Thans
  • Patent number: 10041100
    Abstract: A method of producing sugar solution by repeating a sugar solution production process includes steps (1) to (3), wherein a wash solution obtained in step (4) of washing a separation membrane after step (3) is used for step (1) of subsequent sugar solution production processes: step (1): preparing slurry of pretreated cellulose-containing biomass; step (2): hydrolyzing the slurry of pretreated cellulose-containing biomass in step (1) using cellulase from filamentous fungi; and step (3): subjecting the hydrolyzate of step (2) to solid-liquid separation into a solution component and a hydrolysis residue, filtering the solution component through an ultrafiltration membrane and recovering the cellulase from filamentous fungi as a non-permeate, and recovering the sugar solution as a permeate.
    Type: Grant
    Filed: March 3, 2014
    Date of Patent: August 7, 2018
    Assignee: Toray Industries, Inc.
    Inventors: Chiaki Yamada, Hiroyuki Kurihara, Katsushige Yamada
  • Patent number: 10041101
    Abstract: The present invention relates to compositions comprising: a polypeptide having cellulolytic enhancing activity and a heterocyclic compound. The present invention also relates to methods of using the compositions.
    Type: Grant
    Filed: June 27, 2016
    Date of Patent: August 7, 2018
    Assignee: Novozymes, Inc.
    Inventors: Jason Quinlan, Feng Xu, Matthew Sweeney
  • Patent number: 9994873
    Abstract: The present invention describes a process for the production of alcohols and/or solvents from a cellulosic or lignocellulosic substrate, comprising at least the following steps: a step A for alkaline pre-treatment of said substrate, comprising a step for heating in the presence of an alkaline chemical reagent and an optional washing step; a step B constituted by a step for adjusting the pH and a step for enzymatic hydrolysis of the pre-treated substrate, at the end of which a hydrolysate is obtained constituted by a liquid phase containing sugars and a solid residue; a step for alcoholic fermentation of the hydrolysate obtained; a step for separation/purification (step C2) at the end of which one or more purified alcohols and/or solvents are obtained; a step for extracting the solid residue; a step D for acid digestion of at least a fraction of the extracted solid residue, at the end of which a portion or all of the product obtained is recycled to step B.
    Type: Grant
    Filed: May 6, 2010
    Date of Patent: June 12, 2018
    Assignee: IFP ENERGIES NOUVELLES
    Inventors: Marcel Ropars, Caroline Aymard
  • Patent number: 9982280
    Abstract: The invention relates to a process for the preparation of a fermentation product from ligno-cellulosic material, comprising the following steps: a) optionally pre-treatment of the ligno-cellulosic material; b) optionally washing of the optionally pre-treated ligno-cellulosic material; c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61; d) whereby less than 7.5 mg enzyme composition/g glucan (on dry matter and enzyme as protein) or less than 3.0 mg enzyme composition/g feedstock (on dry matter and enzyme as protein) is used; and e) fermentation of the hydrolysed ligno-cellulosic material to produce a fermentation product; and f) optionally recovery of a fermentation product; wherein before and/or during the enzymatic hydrolysis oxygen is added to the ligno-cellulosic material.
    Type: Grant
    Filed: November 7, 2013
    Date of Patent: May 29, 2018
    Assignee: DSM IP ASSETS B.V.
    Inventors: Bertus Noordam, Michael Petrus Jozef Berkhout
  • Patent number: 9944915
    Abstract: A cellulase having improved thermostability is disclosed. The cellulase comprises a modified amino acid sequence of SEQ ID NO: 2, wherein the modification is adding a cysteine in N terminal and adding a glycine and a cysteine or adding a proline and a cysteine in C terminal.
    Type: Grant
    Filed: December 16, 2016
    Date of Patent: April 17, 2018
    Assignee: DONGGUAN APAC BIOTECHNOLOGY CO., LTD.
    Inventors: Rey-Ting Guo, Ya-Shan Cheng, Jian-Wen Huang, Tzu-Hui Wu, Hui-Lin Lai, Cheng-Yen Lin, Tsung-Yu Ko
  • Patent number: 9944914
    Abstract: A method for selectively obtaining a natural variant of an enzyme having activity includes (1) a step of detecting an ORF sequence of a protein having enzyme activity from a genome database including base sequences of metagenomic DNA of environmental microbiota; (2) a step of obtaining at least one PCR clone including the ORF sequence having a full length, a partial sequence of the ORF sequence, or a base sequence encoding an amino acid sequence which is formed by deletion, substitution, or addition of at least one amino acid residue in an amino acid sequence encoded by the ORF sequence, by performing PCR cloning on at least one metagenomic DNA of the environmental microbiota by using a primer designed based on the ORF sequence; (3) a step of determining a base sequence and an amino acid sequence which is encoded by the base sequence for each PCR clone obtained in the step (2); and (4) a step of selecting a natural variant of an enzyme having activity by measuring enzyme activity of proteins encoded by each P
    Type: Grant
    Filed: March 10, 2015
    Date of Patent: April 17, 2018
    Assignee: Honda Motor Co., Ltd.
    Inventors: Migiwa Suda, Jiro Okuma, Asuka Yamaguchi, Yoshitsugu Hirose, Yasuhiro Kondo
  • Patent number: 9938552
    Abstract: The invention relates to methods of saccharifying a cellulosic material comprising subjecting the cellulosic material to a catalase and a cellulolytic enzyme composition comprising a GH61 polypeptide in the presence of dissolved oxygen at a concentration of greater than 10% of the saturation level. The invention also related to methods of producing desired fermentation products, such as ethanol, using a method including a saccharification step of the invention.
    Type: Grant
    Filed: October 31, 2014
    Date of Patent: April 10, 2018
    Assignee: Novozymes A/S
    Inventors: Hui Xu, Jesper Frickman, Jiyin Liu, Katja Salomon Johansen, Hong Zhi Huang
  • Patent number: 9920345
    Abstract: Methods of processing lignocellulosic biomass to fermentable sugars are provided which rely on hydrothermal pretreatment. Soft lignocellulosic biomass feedstock is pretreated in a single-stage pressurized hydrothermal pretreatment to very low severity. The pre-treated biomass is hydrolysed, typically as a whole slurry, using enzymatic hydrolysis catalysed by an enzyme mixture comprising endoglucanase, exoglucanase, ?-glucosidase, endoxylanase, and ?-xylosidase activities at activity levels in nkat/g glucan of endoglucanase of at least 1100, exoglucanase of at least 280, ?-glucosidase of at least 3000, endoxylanase of at least 1400, and ?-xylosidase of at least 75, so as to produce a hydrolysate in which the yield of C5 monomers is at least 55% of the original xylose and arabinose content of the feedstock prior to pretreatment.
    Type: Grant
    Filed: February 5, 2014
    Date of Patent: March 20, 2018
    Assignee: INBICON A/S
    Inventors: Jan Larsen, Niels Nielsen Poulsen, Martin Dan Jeppesen, Kit Kellebjerg Mogensen
  • Patent number: 9879244
    Abstract: The present invention relates to xylanase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Grant
    Filed: May 30, 2014
    Date of Patent: January 30, 2018
    Assignee: NOVOZYMES A/S
    Inventor: Mark Wogulis
  • Patent number: 9856465
    Abstract: The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast Saccharomyces cerevisiae. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.
    Type: Grant
    Filed: March 8, 2016
    Date of Patent: January 2, 2018
    Assignee: Lallemand Hungary Liquidity Management LLC
    Inventors: Elena E. Brevnova, Vineet Rajgarhia, Mark Mellon, Anne Warner, John McBride, Chhayal Gandhi, Erin Wiswall
  • Patent number: 9834763
    Abstract: The present invention relates to a Myceliophthora thermophila host cell which expresses a recombinant enzymes from Fusarium oxysporum with beta-xylosidase activity. The invention also refers to an enzymatic composition comprising the host cell of the invention and/or the recombinant enzyme with beta-xylosidase activity expressed by the host cell of the invention. The invention further relates to the use of the host cell of the invention, the recombinant enzyme with beta-xylosidase activity expressed by the host cell of the invention or the composition of the invention for the degradation of biomass and to a method of producing bioproducts, preferably bioethanol, which comprises the use of the host cell of the invention, the recombinant enzyme with beta-xylosidase activity expressed by the host cell of the invention or the composition of the invention.
    Type: Grant
    Filed: May 20, 2013
    Date of Patent: December 5, 2017
    Assignee: Abengoa Bioenergia Nuevas Tecnologias, S.A.
    Inventors: Bruno Diez Garcia, Ana Gomez Rodriguez, Jorge Gil Martinez, Noelia Valbuena Crespo, Antonio Javier Moreno Perez, Rafael Duenas Sanchez, Ana Maria Munoz Gonzalez, Dolores Perez Gomez, Lucia Martin Perez, Sandra Gavalda Martin, Laura Sanchez Zamorano, Consolacion Alvarez Nunez, Maria de los Angeles Bermudez Alcantara, Pablo Gutierrez Gomez, Ricardo Arjona Antolin
  • Patent number: 9738881
    Abstract: The invention relates to a host cell comprising at least four different heterologous polynucleotides chosen from the group of polynucleotides encoding cellulases, hemicellulases and pectinases, wherein the host cell is capable of producing the at least four different enzymes chosen from the group of cellulases, hemicellulases and pectinases, wherein the host cell is a filamentous fungus and is capable of secretion of the at least four different enzymes. This host cell can suitably be used for the production of an enzyme composition that can be used in a process for the saccharification of cellulosic material.
    Type: Grant
    Filed: July 8, 2015
    Date of Patent: August 22, 2017
    Assignee: DSM IP ASSETS B.V.
    Inventors: Alrik Pieter Los, Cornelis Maria Jacobus Sagt, Margot Elisabeth Francoise Schoonneveld-Bergmans, Robbertus Antonius Damveld
  • Patent number: 9725742
    Abstract: A process for obtaining high ethanol yield from the fermentation of an energy crop and for producing a nutritionally enhanced feed co-product is provided. In particular, the process includes converting non-fermentable polysaccharides in an energy crop into fermentable sugar. The fermentable sugars may be fermented into ethanol thereby enhancing the ethanol yield. In addition, separation of ethanol from the fermentation product yields a whole stillage product having enhanced protein content and reduced fiber content. The process requires little or no modification to the configuration of existing commercial ethanol facilities.
    Type: Grant
    Filed: May 9, 2013
    Date of Patent: August 8, 2017
    Assignee: Abengoa Bioenergy New Technologies, LLC
    Inventors: Carlos Blázquez Escudero, Pablo Gutiérrez Gómez
  • Patent number: 9675084
    Abstract: Methods are provided for reducing color and/or unpleasant taste and/or malodor development in a composition comprising a plant material, such a cereal bran, in particular during solubilization processes of the plant material. Optimized solubilization methods are provided, wherein color and/or unpleasant taste and/or malodor development is controlled. Solubilized plant material, kit of parts, the use of such solubilized plant material, e.g. in food products or bioethanol are also provided.
    Type: Grant
    Filed: March 29, 2010
    Date of Patent: June 13, 2017
    Assignee: DuPont Nutrition Biosciences ApS
    Inventors: René Mikkelsen, Jens Frisbæk Sørensen