Produced By The Action Of A Carbohydrase (e.g., Maltose By The Action Of Alpha Amylase On Starch, Etc.) Patents (Class 435/99)
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Publication number: 20150037848Abstract: The invention provides a method for producing saccharide, the method including the following steps (1) and (2). In step (1), a cellulose raw material is heated in the presence of one or more additives selected from the group consisting of a nonionic surfactant and polyethylene glycol; and water, at a pH lower than 7, to thereby prepare a heat treatment product. In step (2), the treatment product prepared in step (1) to is subjected to a saccharification treatment by use of an enzyme.Type: ApplicationFiled: February 21, 2013Publication date: February 5, 2015Applicant: KAO CORPORATIONInventor: Toshihiro Yano
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Publication number: 20150037845Abstract: The present disclosure relates to mutant thermostable glycosyl hydrolases family 7 enzymes, including mutant Trichoderma reesei endoglucanase I. In particular, the present disclosure relates to mutant thermostable enzymes, compositions containing the enzymes, and methods of use thereof.Type: ApplicationFiled: August 11, 2011Publication date: February 5, 2015Applicant: The Regents of the University of CaliforniaInventors: Harshal Akshay Chokhawala, Tae-Wan Kim, Harvey W. Blanch, Douglas S. Clark
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Publication number: 20150037846Abstract: Methods and systems for biomass hydrolysis are disclosed. The methods use wash liquor in a sequencing process to maximize sugar yields, particularly C5 saccharides.Type: ApplicationFiled: August 1, 2013Publication date: February 5, 2015Applicant: Renmatix, Inc.Inventors: Manuk Colakyan, Ferhan Kayihan
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Patent number: 8945245Abstract: A method is provided in which pretreated and densified cellulosic biomass particulates can be hydrolyzed at a high solids loading rate as compared with the solids loading rate of loose hydrolysable cellulosic biomass fibers. The resulting high concentration sugar-containing stream can be easily converted to biofuels or an entire suite of other useful bioproducts.Type: GrantFiled: April 27, 2012Date of Patent: February 3, 2015Assignees: The Michigan Biotechnology Institute, Board of Trustees of Michigan State UniversityInventors: Bryan Bals, Farzaneh Teymouri, Tim Campbell, Bruce Dale
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Patent number: 8946407Abstract: Provided are a fructosylated mangiferin, a preparation method therefor and a use thereof, wherein the fructosylated mangiferin has a structural formula represented by the following formula (I), the method for preparing the fructosylated mangiferin includes adding a substance with fructosylating enzymatic activity to a transformed liquid containing mangiferin for biotransformation reaction, so as to convert the mangiferin into the fructosylated mangiferin, wherein the transformed liquid contains the mangiferin and a glycosyl donor; as well as a use of the fructosylated mangiferin in preparation of a medicament for treatment of tumor-related diseases.Type: GrantFiled: March 8, 2012Date of Patent: February 3, 2015Assignee: Nanjing University of TechnologyInventors: Bingfang He, Xueming Wu, Jianlin Chu, Bin Wu, Sen Zhang, Pingkai Ouyang
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Publication number: 20150031091Abstract: The present invention provides isolated polypeptides having alpha-amylase activity catalytic domains, carbohydrate binding domains and polynucleotide encoding the polypeptides, catalytic domains or carbohydrate binding domains. The invention also provides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or carbohydrate binding domains.Type: ApplicationFiled: September 29, 2012Publication date: January 29, 2015Applicant: NOVOZYMES A/SInventors: Ming Li, Junxin Duan, Noriko Tsutsumi, Guillermo Coward-Kelly, Henrik Lundkvist
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Publication number: 20150031082Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: October 13, 2014Publication date: January 29, 2015Inventor: Marc Dominique Morant
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Publication number: 20150031078Abstract: The present invention is a Kluyveromyces lactis yeast strain comprising the sequence identified by SEQ ID NO: 1, and methods for the production of sugars (glucose and galactose), ethanol, ?-galactosidase and biomass, in which said Kluyveromyces lactis yeast strain is cultured in the presence of a lactose-containing medium. The lactose-containing medium may be milk, whey, whey resulting from the preparation of butter, whey resulting after casein precipitation, milk permeate, whey permeate, acid whey and YPL culture medium.Type: ApplicationFiled: July 27, 2011Publication date: January 29, 2015Applicant: QUEIZUAR, S.L.Inventors: Angel Pereira Rodriguez, Manuel Becerra Fernandez, Maria Isabel Gonzalez Siso, Maria Esperanza Cerdan Villanueva
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Publication number: 20150031080Abstract: The present invention relates to variants of a parent cellobiohydrolase II. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: October 13, 2014Publication date: January 29, 2015Inventor: Mark Wogulis
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Publication number: 20150031079Abstract: The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: October 13, 2014Publication date: January 29, 2015Inventor: Nikolaj Spodsberg
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Publication number: 20150031092Abstract: A method for pretreating lignocellulosic biomass by: a) acid hydrolysis with an acid solution resulting in a liquid fraction and a solid fraction, b) separating the solid fraction and the liquid fraction, c) drying the solid fraction, d) firing the dried solid fraction in a medium comprising at least one hydrated inorganic salt to obtain a solid fraction and a liquid fraction, e) separating the solid fraction and the liquid fraction obtained in d), f) optionally, treating said solid fraction obtained in e), g) enzymatic hydrolysis of said solid fraction obtained in e) and/or f), and wherein at least a portion of the liquid fraction obtained in b) is used to grow microorganisms which produce the enzymes necessary for the enzymatic hydrolysis of g).Type: ApplicationFiled: December 11, 2012Publication date: January 29, 2015Applicant: IFP ENERGIES NOUVELLESInventors: Christophe Vallee, Didier Bernard, Caroline Aymard, Helene Olivier-Bourbigou
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Publication number: 20150024438Abstract: The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast Saccharomyces cerevisiae. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.Type: ApplicationFiled: February 24, 2014Publication date: January 22, 2015Applicant: Mascoma CorporationInventors: Elena E. Brevnova, Vineet Rajgarhia, Mark Mellon, Anne Warner, John McBride, Chhayal Gandhi, Erin Wiswall
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Publication number: 20150024439Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: ApplicationFiled: September 29, 2014Publication date: January 22, 2015Inventors: Kimberly Brown, Paul Harris, Elizabeth Zaretsky, Edward Re, Elena Vlasenko, Keith McFarland, Alfredo Lopez de Leon
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Publication number: 20150017690Abstract: The present invention relates to a polypeptide which has ?-glucosidase activity, and which includes an amino acid sequence represented by SEQ ID NO: 1, a polypeptide including an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, or a polypeptide including an amino acid sequence having 91% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1. According to the present invention, a novel ?-glucosidase enzyme derived from Acremonium cellulolyticus, a polynucleotide encoding the ?-glucosidase, an expression vector for expressing the ?-glucosidase, a transformant incorporated with that expression vector, and a method for producing a cellulose degradation product using the ?-glucosidase can be provided.Type: ApplicationFiled: July 7, 2014Publication date: January 15, 2015Inventors: Maiko TANAKA, Shigenobu MITSUZAWA, Satoru SHINKAWA, Daisuke SHIBATA, Takeshi ARA, Migiwa TAKEDA
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Publication number: 20150017692Abstract: The present invention relates to a polypeptide which has ?-glucosidase activity, and which includes an amino acid sequence represented by SEQ ID NO: 1, a polypeptide including an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, or a polypeptide including an amino acid sequence having 92% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1. According to the present invention, a novel ?-glucosidase enzyme derived from Acremonium cellulolyticus, a polynucleotide encoding the ?-glucosidase, an expression vector for expressing the ?-glucosidase, a transformant incorporated with that expression vector, and a method for producing a cellulose degradation product using the ?-glucosidase can be provided.Type: ApplicationFiled: July 7, 2014Publication date: January 15, 2015Inventors: Maiko TANAKA, Shigenobu MITSUZAWA, Satoru SHINKAWA, Daisuke SHIBATA, Takeshi ARA, Migiwa TAKEDA
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Publication number: 20150017691Abstract: The present invention relates to a polypeptide which has ?-glucosidase activity, and which includes an amino acid sequence represented by SEQ ID NO: 1, a polypeptide including an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, or a polypeptide including an amino acid sequence having 92% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1. According to the present invention, a novel ?-glucosidase enzyme derived from Acremonium cellulolyticus, a polynucleotide encoding the ?-glucosidase, an expression vector for expressing the ?-glucosidase, a transformant incorporated with that expression vector, and a method for producing a cellulose degradation product using the ?-glucosidase can be provided.Type: ApplicationFiled: July 7, 2014Publication date: January 15, 2015Inventors: Maiko TANAKA, Shigenobu MITSUZAWA, Satoru SHINKAWA, Daisuke SHIBATA, Takeshi ARA, Migiwa TAKEDA
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Publication number: 20150017689Abstract: The present invention relates to a polypeptide which has ?-glucosidase activity, and which includes an amino acid sequence represented by SEQ ID NO: 1, a polypeptide including an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, or a polypeptide including an amino acid sequence having 90% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1. According to the present invention, a novel ?-glucosidase enzyme derived from Acremonium cellulolyticus, a polynucleotide encoding the ?-glucosidase, an expression vector for expressing the ?-glucosidase, a transformant incorporated with that expression vector, and a method for producing a cellulose degradation product using the ?-glucosidase can be provided.Type: ApplicationFiled: July 3, 2014Publication date: January 15, 2015Inventors: Maiko TANAKA, Shigenobu MITSUZAWA, Satoru SHINKAWA, Daisuke SHIBATA, Takeshi ARA, Migiwa TAKEDA
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Publication number: 20150018584Abstract: Provided are methods and compositions for high yields while using reduced enzyme loads in saccharification and fermentation processes. These methods increase the efficiency of enzymes and result in improved yields and composition of saccharification and fermentation end products.Type: ApplicationFiled: July 24, 2014Publication date: January 15, 2015Inventors: Sarad Parekh, Carl P. Felice
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Publication number: 20150010957Abstract: The Alkcell (alkali-cellulase) Process is a method for the conversion of biomass (cellulosic material) such as agricultural waste, for example, corn stover into glucose. The process uses an alkali pretreatment of biomass followed by hydrolysis with cellulase enzymes to produce glucose. The Alkcell Process uses commonly available materials and methods readily implemented near the biomass growing site. The operating conditions are mild with temperatures not above boiling water solutions, and require half-day conversion to produce glucose in water. Remaining solids can be recycled for additional glucose. An advantage of the Alkcell Process is implementation at or near the biomass growing site that avoids transporting biomass distances to special cellulose processing facilities. The glucose produced can be fermented at existing grain fermenting facilities so that special cellulose handling refineries are not required.Type: ApplicationFiled: July 5, 2013Publication date: January 8, 2015Inventor: John Joseph Savarese
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Publication number: 20150013033Abstract: The present invention provides an expression cassette containing a polynucleotide coding sequence for a hydroxycinnamoyl-CoA hydratase-lyase (HCHL), which is operably linked to a heterologous promoter. Also provided are methods of engineering plants that have reduced lignification, as well as cells, plant parts, and plant tissues from such engineered plants.Type: ApplicationFiled: July 13, 2012Publication date: January 8, 2015Applicant: The Regents of the University of CaliforniaInventors: Dominique Loqué, Amyerick Guillaume Eudes
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Publication number: 20150010959Abstract: A method for producing saccharides containing glucose as a main component is described, including degrading cellulose and/or hemicellulose with a cellulase, wherein an additive containing a protein and an amino acid and/or a yeast lysate solution is added to the cellulose and/or hemicellulose and the cellulase is used to cause an enzymatic saccharification reaction of saccharifying the cellulose and/or hemicellulose.Type: ApplicationFiled: February 16, 2012Publication date: January 8, 2015Applicant: JGC CORPORATIONInventors: Daisuke Taneda, Makoto Ikeo, Shohei Okino
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Publication number: 20150010960Abstract: An object of the present invention is to provide a soft biomass decomposition method, a production method for a target substance from soft biomass, and an enzyme or group of enzymes for decomposing soft biomass. Provided is a soft biomass decomposition method, including a step of bringing an enzyme selected from specific exocellulase, endocellulase, and processive endocellulase into contact with soft biomass such as bagasse and rice straw. Also provided is a production method for a target substance from soft biomass by incorporating the soft biomass decomposition method as a step. Further provided is an enzyme or group of enzymes for decomposing soft biomass selected from specific exocellulase, endocellulase, and processive endocellulase.Type: ApplicationFiled: January 29, 2013Publication date: January 8, 2015Applicant: NATIONAL UNIVERSITY CORPORATION MIE UNIVERSITYInventors: Yutaka Tamaru, Takeshi Katsuyama
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Publication number: 20150010958Abstract: The present invention provides methods for degrading or converting a cellulosic material using an enzyme composition in the presence of a reducing agent. The present invention also provides methods for producing a fermentation product and methods of fermenting a cellulosic material using an enzyme composition in the presence of a reducing agent.Type: ApplicationFiled: January 28, 2013Publication date: January 8, 2015Applicants: Novoztmes A/S, Novozymes Inc.Inventors: Hongzhi Huang, Yun Wang, Wei Li, Feng Xu, Ani Tejirian
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Publication number: 20150010961Abstract: The present invention relates to a family 5 glycoside hydrolase variant having endoglucanase activity, polynucleotides encoding the family 5 glycoside hydrolase variant, vectors, host cells comprising the polynucleotides, and methods for using the family 5 glycoside hydrolase variant.Type: ApplicationFiled: September 25, 2014Publication date: January 8, 2015Inventors: John Poland, Justin T. Stege
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Patent number: 8927235Abstract: The present invention relates to methods of degrading or converting biomass material enriched with hemicellulosic material into fermentable sugars.Type: GrantFiled: December 6, 2011Date of Patent: January 6, 2015Assignee: Novozymes A/SInventors: Prashant Iyer, Harry Showmaker, Hui Xu, Kishore Rane
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Publication number: 20150004654Abstract: In this disclosure, a process for producing biomass pellets and sugars from cellulosic biomass is provided, comprising: extracting the feedstock with steam and/or hot water and optionally with an acid catalyst, to produce cellulose-rich solids and an extract liquor containing hemicellulosic oligomers and lignin; separating the cellulose-rich solids from the extract liquor; filtering the extract liquor to remove at least some of the lignin, thereby generating a filter permeate comprising cleaned extract liquor containing the hemicellulosic oligomers and a filter retentate comprising a lignin-rich stream; hydrolyzing the hemicellulosic oligomers in the cleaned extract liquor with an acid or enzymes, to generate hemicellulosic monomers which are recovered; and pelletizing the cellulose-rich solids to form biomass pellets, wherein the pelletizing utilizes at least some of the lignin-rich stream as a binder or binder component.Type: ApplicationFiled: June 25, 2014Publication date: January 1, 2015Inventors: Theodora RETSINA, Vesa PYLKKANEN
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Publication number: 20150004656Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: September 15, 2014Publication date: January 1, 2015Inventors: Lan Tang, Ye Liu, Junxin Duan, Yu Zhang, Christian Joergensen, Randall Kramer
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Publication number: 20150005484Abstract: A method for saccharification of lignocellulosic biomass, the method comprising (1) a pretreatment step of impregnating lignocellulosic biomass with an aqueous alkali solution, subjecting the resultant mixture to solid-liquid separation to remove part of the aqueous alkali solution, and then performing heat treatment, and (2) a saccharification step of enzymatically degrading the lignocellulosic biomass resulting from the pretreatment step to obtain a saccharified liquid can be applied to high-lignin lignocellulosic biomass, reduce the usage of alkali and water in the pretreatment step, increase the sugar yield in the saccharification step, decrease the reaction time, reduce enzyme adsorption on a biomass residue, and improve the enzyme recovery rate.Type: ApplicationFiled: January 23, 2013Publication date: January 1, 2015Inventor: Takafumi Kubo
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Publication number: 20150004655Abstract: The present invention relates to cellobiohydrolase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of producing and using the variants.Type: ApplicationFiled: December 20, 2012Publication date: January 1, 2015Inventor: Mark Wogulis
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Publication number: 20150007369Abstract: The present invention relates to GH61 polypeptide variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: November 21, 2012Publication date: January 1, 2015Inventors: Janine Lin, Doreen Bohan, Michelle Maranta, Leslie Beresford, Michael Lamsa, Bjarne Gram Hansen, Frank Winther Rasmussen, Matt Sweeney, Douglas J. Boyle, III
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Publication number: 20140377811Abstract: The present invention concerns micro-organisms which present cellulases on their surface. Corresponding micro-organisms were produced with the aid of corresponding plasmids which encode a section comprising a signal peptide, a heterologous cellulase, an optional protease recognition site, a transmembrane linker and a transporter domain of an autotransporter or a variant thereof. Such micro-organisms were advantageously used in the conversion of cellulose into cellobiose and/or glucose. It was also possible to recover the micro-organisms from the reaction mixture following conversion from simple substrates. Also, a combination of various micro-organisms, which were populated with exocellulases, endocellulases and beta-glucosidases, were used to produce glucose from cellulose or wood.Type: ApplicationFiled: March 11, 2014Publication date: December 25, 2014Applicant: ZYRUS Beteiligungsgesellschaft mbH & Co. Patente I KGInventors: Michael Jurgens, Andreas Rohardt, Ruth Maas, Tatjana Brossette
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Publication number: 20140377812Abstract: The present invention concerns a production of liquefied lignocellulosic substrate by enzymatic reaction. 10% to 40% by weight dry matter of pre-treated lignocellulosic substrate is contacted, with water and enzymes at 0.1 to 60 mg of enzymes per gram of cellulose for a period of 1 to 24 hours. Over time, at least the value of one of the rheological characteristics of the reaction medium is measured. If a reduction of the value is detected over time, the following step a) is carried out: a) the feeding flow rate of pre-treated lignocellulosic substrate is increased, with or without modification of the flow rate of enzymes and/or water; If an increase of the value is detected over time, a step b) is carried out: b) the feeding flow rate of water and/or enzymes is increased, with or without modification of the flow rate of pre-treated lignocellulosic substrate.Type: ApplicationFiled: November 29, 2012Publication date: December 25, 2014Applicant: IFP ENERGIES NOUVELLESInventors: Sylvain Louret, Romain Rousset
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Publication number: 20140370551Abstract: This disclosure provides a business method and system for generating sugars and recycling a non-biomass component from a waste stream. In some embodiments, a waste stream comprising cellulose and a non-biomass component is saccharified to produce glucose, followed by recovery of the glucose and non-biomass component, which may be recycled to another site associated with production of a cellulose-containing product that contains the non-biomass component. In certain scenarios, the waste stream is generated at a first location, cellulose pretreatment (if desired) and hydrolysis are conducted at a second location, and the non-biomass component is recycled to the first location or a third location. The non-biomass component may include metals, metal oxides, salts, organic compounds, inorganic compounds, oligomers, or polymers, for example.Type: ApplicationFiled: June 16, 2014Publication date: December 18, 2014Inventors: Theodora RETSINA, Kimberly NELSON
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Publication number: 20140370553Abstract: The present invention relates to polypeptides comprising a carbohydrate-binding module amino acid sequence and an alpha-amylase amino acid sequence as well as to the application of such polypeptides.Type: ApplicationFiled: August 15, 2014Publication date: December 18, 2014Inventors: Shiro Fukuyama, Tomoko Matsui, Chee Leong Soong, Eric Allain, Anders Vikso Nielsen, Hiroaki Udagawa, Ye Liu, Junxin Duan, Wenping Wu, Lene Nonboe Andersen
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Publication number: 20140370552Abstract: The present invention provides endoglucanase 1b (EG1b) variants suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce EG1b variants, as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures.Type: ApplicationFiled: December 18, 2012Publication date: December 18, 2014Inventors: Ee Lui Ang, Ellen D. Eberhard, Xiyun Zhang, Wei Zhang, Jing Tian, Derek J. Smith, Vesna Mitchell
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Patent number: 8911976Abstract: The invention relates to an enzyme stabilizer comprising a lignin derivative produced by reaction between lignin and a hydrophilic compound, and to a method of saccharifying lignocellulosic biomass which employs the enzyme stabilizer. According to the invention it is possible to accomplish effective saccharification of cellulosic biomass with saccharifying enzymes, by enhancing saccharifying enzyme activity and preventing nonspecific adsorption of saccharifying enzyme onto substrate.Type: GrantFiled: March 7, 2011Date of Patent: December 16, 2014Assignee: Forestry and Forest Products Research InstituteInventors: Yasumitsu Uraki, Harumi Honma, Tatsuhiko Yamada, Satoshi Kubo, Masanobu Nojiri
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Publication number: 20140363855Abstract: A process for increasing alcohol yield from biomass (the form or agro- or forest residue, grains, hops, etc.), involving multiple hydrodynamic cavitation treatments of biomass filtrate—both before and after fermentation. Carbohydrates extracted from biomass are subjected to a first cavitation treatment to promote additional conversion into carbohydrates. The carbohydrates are then combined with bacterial species and nutrients, and allowed to ferment. The fermentation product is subjected to a second hydrodynamic cavitation treatment to promote further conversion of carbohydrates into bioalcohol. After distillation, the bioalcohol is subjected to a second hydrodynamic cavitation treatment to increase its purity.Type: ApplicationFiled: August 22, 2014Publication date: December 11, 2014Inventors: Roman Gordon, Igor Gorodnitsky, Maxim A. Promtov, Vijayanand Suryakant Moholkar
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Publication number: 20140356913Abstract: A strategy for eliminating or greatly reducing the need for physical/chemical treatments or the use of whole microbes for lignocellulosic biomass and organopollutant degradation is disclosed. The soybean is a practical, cost-efficient and sustainable bioreactor for the production of lignin-degrading and cellulose-degrading enzymes. The use of soybean as a transgenic overexpression platform provides advantages that no other industrial scale enzyme expression system can match. Availability of a battery of related plant biomass degrading enzymes in separate transgenic soybean lines provides unprecedented flexibility in industrial and bioremediation processes. Depending upon the particular application, selected soybean-derived powdered enzyme formulations can be used, and their sequential addition can be orchestrated.Type: ApplicationFiled: March 29, 2014Publication date: December 4, 2014Inventors: Matthew Wayne Parrow, Kenneth John Piller, Kenneth Lee Bost
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Publication number: 20140356914Abstract: The present invention provides endoglucanase 1b (EG1b) suitable for use in saccharification reactions.Type: ApplicationFiled: August 27, 2012Publication date: December 4, 2014Applicant: Codexis, Inc.Inventors: Kripa Rao, Xiyun Zhang, Brian R. Scott, John J. Tomashek
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Publication number: 20140342410Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: May 14, 2014Publication date: November 20, 2014Applicant: Novozymes Inc.Inventor: Nikolaj Spodsberg
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Publication number: 20140342408Abstract: Provided are isolated polypeptides having xylanase activity, catalytic domains and cellulose binding domains, and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains.Type: ApplicationFiled: November 22, 2012Publication date: November 20, 2014Inventors: Yu Zhang, Lan Tang, Junxin Duan, Ye Liu
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Publication number: 20140343273Abstract: The present invention pertains to methods for preparing enzymatically hydrolyzed starch for use as a stabilizing agent that include the steps of first gelatinizing a starch and next, hydrolyzing the gelatinized starch with an enzyme having endo-hydrolytic activity. The present invention also pertains to the resulting enzymatically hydrolyzed starch for use as a stabilizing agent within emulsions, beverages, food products and industrial products prepared using the enzymatically hydrolyzed starch.Type: ApplicationFiled: July 25, 2014Publication date: November 20, 2014Applicant: CARGILL, INCORPORATEDInventors: Dirk FONTEYN, John R. HEIGIS, Joseph Bernard HOLTWICK, Catharina Hillagonda HOMSMA, David J. MAURO, Dirk Reimond PROVOOST, Wen-Juin SHIEH, Blair C. STEPHENS
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Publication number: 20140339467Abstract: Disclosed in this specification is a lignin composition having unique characteristics relative to its characteristics as found in its natural environment. The lignin has been modified so that more lignin decomposes at the lower lignin decomposition temperature than decomposes at the higher lignin decomposition temperature and the lignin composition has a very high surface area relative to naturally occurring lignin compositions.Type: ApplicationFiled: December 20, 2012Publication date: November 20, 2014Inventors: Guliz Arf Elliott, Danilo De Faveri, Francesco Cherchi, Simone Ferrero, Paolo Torre
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Publication number: 20140335571Abstract: Disclosed herein are methods that involve degrading materials that include lignin. In one aspect, the method generally includes contacting a lignin sample with a lignin depolymerase and contacting the lignin sample with an auxiliary compound. In another aspect, the method generally includes contacting an auxiliary compound with a sample that includes uncleaved lignin macromolecules and at least partially cleaved lignin components in intermolecular association with other lignin components and/or at least a portion of the uncleaved lignin macromolecules. In both aspects, the auxiliary compound dissociates at least partially cleaved lignin components from one another and/or the uncleaved lignin macromolecules.Type: ApplicationFiled: December 12, 2012Publication date: November 13, 2014Inventors: Yi-ru Chen-Sarkanen, Simo Sarkanen, Yun-Yan Wang
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Publication number: 20140335570Abstract: The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: May 8, 2014Publication date: November 13, 2014Applicant: Novozymes Inc.Inventor: Nikolaj Spodsberg
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Patent number: 8883457Abstract: Described are compositions and methods relating to starch processing without a phytase pretreatment step and without adjustment of the slurry pH adjustment.Type: GrantFiled: January 9, 2013Date of Patent: November 11, 2014Assignee: Danisco US Inc.Inventors: Suzanne Breneman, Thomas S. Green, Michael Jay Pepsin, Vivek Sharma, Jayarama K. Shetty
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Patent number: 8883456Abstract: The invention relates to a process of process of producing a fermentation product, comprising: liquefying a starch-containing material to dextrins with an alpha-amylase in the presence of a GH61 polypeptide; saccharifying the dextrins to a sugar with a glucoamylase; and fermenting the sugar using a fermenting organism.Type: GrantFiled: March 30, 2011Date of Patent: November 11, 2014Assignee: Novozymes North America, Inc.Inventors: Chee-Leong Soong, Jeremy Saunders
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Publication number: 20140329284Abstract: The present invention relates to chimeric polypeptides having beta-glucosidase activity.Type: ApplicationFiled: September 28, 2012Publication date: November 6, 2014Inventors: Mark Wogulis, David Osborn, Tia Heu
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Publication number: 20140331364Abstract: Provided are isolated polypeptides having beta-glucosidase activity and polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: December 19, 2012Publication date: November 6, 2014Inventors: Ye Liu, Junxin Duan, Yu Zhang, Lan Tang
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Publication number: 20140322753Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: ApplicationFiled: June 30, 2014Publication date: October 30, 2014Inventors: Paul Harris, Elena Vlasenko, Marcus Sakari Kauppinnen, Elizabeth Zaretsky, Sarah Teter, Kimberly Brown