Produced By The Action Of A Carbohydrase (e.g., Maltose By The Action Of Alpha Amylase On Starch, Etc.) Patents (Class 435/99)
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Publication number: 20120151633Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: ApplicationFiled: February 17, 2012Publication date: June 14, 2012Applicants: NOVOZYMES, INC., NOVOZYMES A/SInventors: Søren Flensted Lassen, Paul Harris, Elena Vlasenko, Kristian Bertel Romer Morkeberg Krogh
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Publication number: 20120149070Abstract: The present invention relates to combinatorial variants of a parent glucoamylase that have altered properties for reducing the synthesis of condensation products during hydrolysis of starch. Accordingly the variants of a parent glucoamylase are suitable such as for use within brewing and glucose syrup production. Also disclosed are DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.Type: ApplicationFiled: August 18, 2010Publication date: June 14, 2012Applicants: DANISCO US INC., DANISCO A/SInventors: Peter Edvard Degn, Richard Bott, Casper Willem Vroemen, Martijn Silvan Scheffers, Wolfgang Aehle
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Patent number: 8193324Abstract: A modular process for organosolv fractionation of lignocellulosic feedstocks into component parts and further processing of said component parts into one or more of a de-lignified cellulose stream, a sugar stream, small-chain alcohol streams and four structurally distinct classes of lignin derivatives. The modular process comprises a first processing module configured for digesting lignocellulosic feedstocks with an organic solvent thereby producing a cellulosic solids fraction and a liquid fraction, a second processing module configured for recovering small-chain alcohols and optionally a first class of lignin derivatives from the cellulosic solids fraction, a third processing module configured for recovering from the liquid fraction at least one of a second class and a third class of lignin derivatives or mixtures thereof, and waste stream comprising a fourth class of lignin derivatives. The fourth processing module may optionally recover the fourth class of lignin derivatives.Type: GrantFiled: November 26, 2008Date of Patent: June 5, 2012Assignee: Lignol Innovations Ltd.Inventors: Christer Hallberg, Donald O'Connor, Michael Rushton, Edward Kendall Pye, Gordon Gjennstad, Alex Berlin, John Ross MacLachlan, Raymond Ma
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Publication number: 20120135474Abstract: The present disclosure provides methods for the conversion of hemicellulose into fermentable sugars using enzymes isolated from Prevotella bryantii. Hemicellulose-degrading enzymes include an endoxylanase, a ?-xylosidase, a bifunctional ?-xylosidase and ?-glucosidase, a bifunctional arabinofuranosidase and ?-xylosidase, a glucuronidase, and an acetyl xylan esterase. The enzymes can be used to release sugars present in hemicellulose for subsequent fermentation to produce value-added products such as ethanol.Type: ApplicationFiled: April 27, 2010Publication date: May 31, 2012Applicant: The Board of Trustees of the University of IllinoisInventors: Isaac K. O. Cann, Shinichi Kiyonari, Dylan Dodd, Yejun Han, Roderick I. Mackie
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Publication number: 20120135475Abstract: The invention is directed to a liquefied sugar beet and/or sugar cane biomass material as well as production methods and uses thereof. The liquefied biomass is storage stable and can be used for the production of a product resulting from fermentation.Type: ApplicationFiled: May 21, 2010Publication date: May 31, 2012Applicant: SUD-CHEMIE AGInventors: Andre Koltermann, Markus Rarbach, Thomas Bruck, Jochen Gerlach, Isabel Unterstrasser, Andreas Kohl, Zdravko Dragovic, Ulrich Kettling
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Publication number: 20120129227Abstract: The present invention provides for a composition comprising an ionic liquid and a thermostable cellulose, and a method of hydrolyzing a cellulose, comprising: (a) providing a composition comprising a solution comprising an ionic liquid and a cellulose, and (b) introducing a thermostable cellulase to the solution, such that the cellulose is hydrolyzed by the cellulase. The present invention also provides for a Thermatoga maritima thermostable cellulase mutant with increased cellulase activity.Type: ApplicationFiled: April 23, 2010Publication date: May 24, 2012Applicants: Sandia Corporation, The Regents of the University of CaliforniaInventors: Rajat Sapra, Supratim Datta, Zhiwei Chen, Bradley M. Holmes, Blake A. Simmons, Harvey W. Blanch
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Publication number: 20120129226Abstract: A maltogenic a-amylase from Trichoderma reesei (TrAA) and variants thereof are useful in the production of high-maltose syrups from liquefied starch. Particularly high maltose concentrations are achieved when TrAA is used in the presence of a pullulanase. Expression hosts and encoding nucleic acids useful for producing TrAA and its variants also are provided.Type: ApplicationFiled: March 12, 2008Publication date: May 24, 2012Inventors: Gang Duan, Kathy Qian, Martijn Scheffers, Jayarama K. Shetty, Pieter Van Solingen
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Publication number: 20120122162Abstract: This disclosure relates to a method for performing high solids saccharification comprising by (a) providing a cellulosic biomass; (b) pretreating the cellulosic biomass in a pretreatment process to produce a pretreated cellulosic biomass; (c) adjusting said pretreated cellulosic biomass to a solids concentration of 6% to 35% w/w and a starting pH of between 5-7; and (d) hydrolyzing the pretreated biomass with at least one aqueous hydrolyzing liquid comprising at least one enzyme selected from the group consisting of a cellulase, a saccharification enzyme, and a combination thereof for a period of time, to hydrolyze at least a part of the pretreated cellulosic biomass to a cellulosic hydrolysate, said cellulosic hydrolysate comprising fermentable sugars.Type: ApplicationFiled: November 14, 2011Publication date: May 17, 2012Applicant: Andritz Inc.Inventors: Rodolfo ROMERO, Bertil Stromberg
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Patent number: 8178759Abstract: Barley with reduced SSII activity has a starch structure with reduced amylopectin content and a consequent high relative amylose content. Additionally the grain can have a relatively high ? glucan content. The structure of the starch may also be altered in a number of ways which can be characterized by having a low gelatinzation temperature but with reduced swelling. The viscosity of gelatinized starch of the starch is also reduced. There is a chain length distribution of the amylopectin content and a low crystallinity of the starch. The starch is also characterized by having high levels of lipid associated starch exhibiting very high levels of V form starch crystallinity. The dietary fiber content of the starch is high. This has desirable dietary and food processing characteristics.Type: GrantFiled: May 10, 2010Date of Patent: May 15, 2012Assignee: Commonwealth Scientific and Industrial Research OrganisationInventors: Matthew Kennedy Morell, David Topping, Ian Leslie Batey
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Patent number: 8178323Abstract: The invention relates to an emulsifier, a method for preparing said emulsifier, and to its use in various applications, primarily food and cosmetic applications. The invention also relates to the use of said emulsifier for the creation of an elastic, gelled foam. An emulsifier according to the invention is based on a starch which is enzymatically converted, using a specific type of enzyme, and modified in a specific esterification reaction.Type: GrantFiled: July 10, 2006Date of Patent: May 15, 2012Assignee: Coöperatie AVEBE U.A.Inventors: Hendrik Jan De Vries, Cindy Semeijn, Pieter Lykle Buwalda
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Patent number: 8173409Abstract: The present invention is directed to novel acid proteases and more specifically to NSP24 family proteases and NSP25 family proteases including biologically active fragments thereof and to nucleic acid molecules encoding said proteases. Also provided are vectors and host cells including nucleic acid sequences coding for the proteases, methods for producing the proteases, enzyme compositions and methods employing said proteases.Type: GrantFiled: October 20, 2009Date of Patent: May 8, 2012Assignee: Danisco US Inc.Inventors: Kathleen A. Clarkson, Nigel Dunn-Coleman, Suzanne E. Lantz, Craig E. Pilgrim, Piet Van Solingen, Michael Ward
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Patent number: 8173404Abstract: A process for obtaining fermentable sugars from barley, involving treating hulled barley with H2SO4 to produce acid treated barley and treating the acid treated barley with enzymes to produce fermentable sugars.Type: GrantFiled: March 17, 2009Date of Patent: May 8, 2012Assignee: The United States of America, as represented by the Secretary of AgricultureInventors: Tae Hyun Kim, Kevin Hicks, Frank Taylor
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Publication number: 20120107880Abstract: The present invention provides improved fungal strains. In some embodiments, the improved fungal strains find use in hydrolyzing cellulosic material to glucose.Type: ApplicationFiled: November 1, 2011Publication date: May 3, 2012Applicant: CODEXIS, INC.Inventors: Dipnath Baidyaroy, Ish Kumar Dhawan
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Publication number: 20120096597Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: January 28, 2010Publication date: April 19, 2012Applicants: Novozymes, Inc., Novozymes A/SInventors: Kirk Schnorr, Michael Rey
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Publication number: 20120094333Abstract: The present invention relates to a process for enzymatic hydrolysis of granular starch into a soluble starch hydrolysate at a temperature below the initial gelatinization temperature of said granular starch.Type: ApplicationFiled: December 21, 2011Publication date: April 19, 2012Applicant: NOVOZYMES A/SInventors: Anders Vikso-Nielsen, Carsten Andersen, Sven Pedersen, Carsten Hjort
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Publication number: 20120088277Abstract: The invention relates to a variant of a parent fungal glucoamylase, which exhibits improved thermal stability and/or increased specific activity using saccharide substrates.Type: ApplicationFiled: December 19, 2011Publication date: April 12, 2012Applicant: NOVOZYMES A/SInventors: Bjarne Roenfeldt Nielsen, Allan Svendsen, Henrik Pedersen, Jesper Vind, Hanne Vang Hendriksen, Torben Peter Frandsen
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Publication number: 20120083019Abstract: The invention provides recombinant GH61 proteins obtained from Myceliophtora thermophila, and nucleic acids that encode such proteins. The invention also provides protein fractions isolated from M. thermophila supernatant that have GH61 protein activity. These preparations can be used to increase yield of products from reactions in which a cellulose-containing substrate undergoes saccharification by one or more cellulase enzymes, such as endoglucanase, ?-glucosidase, or cellobiohydrolase. Combinations of GH61 protein and cellulases can be used to break down cellulosic biomass into fermentable sugars in the production of ethanol.Type: ApplicationFiled: August 22, 2011Publication date: April 5, 2012Applicant: Codexis, Inc.Inventors: Dipnath Baidyaroy, Louis Clark, Onorato Campopiano, Kripa Rao, Lorand Szabo, Janos Torok
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Publication number: 20120070864Abstract: Methods and composition for biofuel processing are provided. For example, in certain aspects methods for using metal compounds to enhance cellulose enzymatic hydrolysis are described. Furthermore, the invention provides integrated processes with fewer lignin removal steps to improve efficiency.Type: ApplicationFiled: August 23, 2011Publication date: March 22, 2012Inventors: Hao Liu, Junyoug Zhu
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Publication number: 20120064579Abstract: This present disclosure relates to methods for improved production of proteins from a cell culture, particularly to culture components and conditions that can preferentially increase the expression of proteins produced from genes under the control of xylanase gene promoter sequences. The improved methods can be used for the production of enzyme compositions with enhanced xylanase and hemicellulolytic activity.Type: ApplicationFiled: April 30, 2010Publication date: March 15, 2012Inventors: Aaron Kelley, Chuanbin Liu, Colin Mitchinson
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Publication number: 20120058523Abstract: The present invention is directed to improved systems and methods for reducing costs and increasing yields of cellulosic ethanol. In particular, the present invention provides plants genetically transformed for increased biomass, expression of lignocellulolytic enzyme polypeptides, and/or simplification of harvesting and downstream processing. Also provided are methods for processing biomass from these transgenic plants that involve less severe and/or less expensive pre-treatment protocols that are typically employed. Such methods allow, among other things, reduced costs associated with externally applied lignocellulolytic enzyme polypeptides.Type: ApplicationFiled: February 17, 2010Publication date: March 8, 2012Applicant: EDENSPACE SYSTEMS CORPORATIONInventors: Kirk Pappan, Deisy Corridor, Ramesh Nair, Michael Blaylock, Forrest Chumley, David Lee, Bruce Ferguson
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Publication number: 20120040410Abstract: Hemicellulase (xylanase) enzymes possessing endo-xylanase, laminarase, mannanase, arabinase and arabinofuranosidase activity are useful to degrade hemicellulose and other substrates to their constituent sugars.Type: ApplicationFiled: January 19, 2010Publication date: February 16, 2012Applicant: THE BOARD OF REGENTS FOR OKLAHOMA STATE UNIVERSITYInventors: Rolf A. Prade, Fabio M. Squina
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Publication number: 20120040411Abstract: There is disclosed a process for the hydrolysis of cellulose comprising the sequential steps (a) mixing cellulose with a viscosity below 900 ml/g with an aqueous solution to obtain a liquid, wherein particles comprising cellulose in said liquid have a diameter of maximum 200 nm, wherein the temperature of the aqueous solution is below 35° C., and wherein the pH of the aqueous solution is above 12, (b) subjecting the liquid to at least one of the steps: (i) decreasing the pH of the liquid with at least 1 pH unit and (ii) increasing the temperature by at least 20° C., and (c) hydrolyzing the cellulose. Moreover there is disclose glucose manufactured according to the method and ethanol manufactured from the glucose. Advantages include that the cellulose is hydrolyzed faster and to a greater extent after the treatment. The yield is increased.Type: ApplicationFiled: April 16, 2010Publication date: February 16, 2012Applicant: HELI INOVATIO HANDELSBOLAGInventors: Gunnar Henriksson, Mikael Lindstrom
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Publication number: 20120040412Abstract: In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used as amylases to catalyze the hydrolysis of starch into sugars.Type: ApplicationFiled: October 27, 2011Publication date: February 16, 2012Applicant: VERENIUM CORPORATIONInventors: Kevin A. Gray, Nahla Aboushadi, James B. Garrett
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Publication number: 20120040408Abstract: Improved systems and methods for reducing costs and increasing yields of cellulosic ethanol are disclosed herein, along with plants genetically transformed for increased biomass, expression of lignocellulolytic enzyme polypeptides, and/or simplification of harvesting and downstream processing. Methods for processing biomass from these transgenic plants that involve less severe and/or less expensive pre-treatment protocols than are typically employed are also disclosed.Type: ApplicationFiled: June 22, 2009Publication date: February 16, 2012Inventors: Stephen R. Decker, Michael J. Selig, Roman Brunecky, Todd Vinzant, Michael E. Himmell, David Lee, Michael Blaylock
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Publication number: 20120040409Abstract: Thermophilic gram-positive anaerobic host cells, for example Thermoanaerobacterium saccharolyticum (“T sacch”), express heterologous biomass degrading enzymes, such as cellulases, and are able to produce useful fermentation products from cellulose. Useful fermentation products include, for example, ethanol, acetic acid, lactic acid or CO2. In order to provide maximum expression and activity levels, biomass degrading enzymes can be expressed from codon-optimized nucleotide sequences, can be expressed under the control of a high-efficiency promoter, and/or can be fused to a signal peptide. In addition, the host cell, for example, a T sacch host cell, can be genetically altered to further improve ethanol production, for example by disrupting the production of organic products other than ethanol.Type: ApplicationFiled: December 23, 2009Publication date: February 16, 2012Applicant: Mascoma CorporationInventors: Heídi Hau, Chales Rice, Chris Herring, John Mcbride, Arhur J. Shaw. IV, Erin Wiswall
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Patent number: 8110380Abstract: A starch-based biodegradable material composition includes: an enzyme-hydrolyzed starch; and a biodegradable polyester selected from at least one of an aliphatic polyester of polybutylene succinate and an aliphatic-aromatic copolyester. The enzyme-hydrolyzed starch is prepared by hydrolyzing a native starch using a starch-hydrolyzing enzyme. The starch-hydrolyzing enzyme has an activity unit ranging from 15000 to 40000.Type: GrantFiled: April 29, 2008Date of Patent: February 7, 2012Assignee: Grace Biotech CorporationInventors: C. Will Chen, Ching-Huang Wang, Chin-Wei Chen, Yun-Ping Wang
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Patent number: 8101392Abstract: The invention relates to a variant of a parent fungal glucoamylase, which exhibits improved thermal stability and/or increased specific activity using saccharide substrates.Type: GrantFiled: October 15, 2010Date of Patent: January 24, 2012Assignee: Novozymes A/SInventors: Bjarne Roenfeldt Nielsen, Allan Svendsen, Henrik Pedersen, Jesper Vind, Hanne Vang Hendriksen, Torben Peter Frandsen
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Patent number: 8101024Abstract: Disclosed is a method for removing minerals from a cellulosic biomass. For example, the biomass may be prewashed with an acid solution and rinsed with water to remove minerals prior to acid saccharification. The removal of minerals may reduce overall acid requirements, and decrease pretreatment costs.Type: GrantFiled: February 5, 2009Date of Patent: January 24, 2012Assignee: The Trustees of Dartmouth CollegeInventors: Charles E. Wyman, Todd A. Lloyd
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Publication number: 20120015407Abstract: Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of at least partially degrading, cleaving, or removing polysaccharides, lignocellulose, cellulose, hemicellulose, lignin, starch, chitin, polyhydroxybutyrate, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups using isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius.Type: ApplicationFiled: November 15, 2010Publication date: January 19, 2012Applicant: Battelle Energy Alliance, LLCInventors: David N. Thompson, William A. Apel, Vicki S. Thompson, David W. Reed, Jeffrey A. Lacey, Emily D. Henriksen
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Publication number: 20120015408Abstract: The invention provides variants of the Thermoanaerobacter brockii CglT beta-glucosidase that have improve beta-glucosidase activity compared to the wild type enzyme. The invention also provides polynucleotides that encode the variants, as well as methods of producing the variants, enzyme compositions comprising the variants, and methods for using the variants in industrial applications.Type: ApplicationFiled: February 26, 2010Publication date: January 19, 2012Applicant: CODEXIS, INC.Inventors: Dipnath Baidyaroy, Louis Clark, Lisa M. Newman, Charlene Ching
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Publication number: 20120003704Abstract: Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce ethanol and/or butanol, e.g., by fermentation.Type: ApplicationFiled: September 15, 2011Publication date: January 5, 2012Applicant: XYLECO, INC.Inventor: Marshall MEDOFF
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Publication number: 20120003703Abstract: The invention relates to recombinant expression of variant forms of C1 CBH1a and homologs thereof, having improved thermostability, low-pH tolerance, specific activity and other desirable properties. Also provided are methods for producing ethanol and other valuable organic compounds by combining cellobiohydrolase variants with cellulosic materials.Type: ApplicationFiled: May 13, 2011Publication date: January 5, 2012Applicant: Codexis, Inc.Inventors: Vesna Mitchell, Grzegorz Wojciechowski, Oscar Alvizo
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Publication number: 20120003701Abstract: The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast Saccharomyces cerevisiae. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.Type: ApplicationFiled: July 7, 2009Publication date: January 5, 2012Applicant: MASCOMA CORPORATIONInventors: Elena E. Brevnova, Vineet Rajgarhia, Mark Mellon, Anne Warner, John Mcbride, Chhayal Gandhi, Erin Wiswall
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Publication number: 20120003702Abstract: A process for producing a saccharified solution, by which xylose can be produced at a high yield from lignocellulosic biomass, is provided. The saccharification is carried out by adding a saccharifying enzyme to a pretreated material for saccharification of the lignocellulosic biomass. As the saccharifying enzyme is added a mixture of a first saccharifying enzyme originated from one or more microorganisms selected from the group consisting of fungi of the genus Acremonium, the genus Trichoderma, the genus Penicillium, the genus Aspergillus and the genus Thermoascus, and eubacteria of the genus Clostridium and the genus Bacillus, and a second saccharifying enzyme composed of ?-xylosidase originated from Thermotoga maritima.Type: ApplicationFiled: May 9, 2011Publication date: January 5, 2012Applicants: THERMOSTABLE ENZYME LABORATORY CO., LTD., HONDA MOTOR CO., LTD.Inventors: Shigenobu MITSUZAWA, Minako Onodera, Maiko Fukuura, Koh Shikata, Yasuhiro Kashima
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Publication number: 20110300586Abstract: Described herein are improved methods of pretreating lignocellulosic biomass. One aspect of the invention relates to a two-stage pretreatment process comprising a relatively low severity steam treatment, a controlled pH pretreatment, or autohydrolysis, followed by hydrolysis with dilute acid or hot water at a relatively low temperature. In certain embodiments, the methods increase hemicellulose sugar yields, substrate digestibility, and suitability for fermentation as compared to steam explosion or acid hydrolysis alone. The two-stage pretreatment processes also employ few chemicals, minimizing the costs associated with pretreatment of lignocellulosic biomass. Moreover, the two-stage pretreatment process may expand the range of suitable feedstocks for bioethanol production.Type: ApplicationFiled: December 18, 2009Publication date: December 8, 2011Inventors: Chaogang Liu, Kevin Wenger
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Publication number: 20110300585Abstract: The various embodiments of the present invention relate generally to compositions, systems, and methods for altering rates of catalysis. More particularly, the various embodiments of the present invention are directed toward compositions, systems, and methods for enzymatic hydrolysis of polysaccharides, such as cellulose and starch. An aspect of the present invention comprises a method for altering the rate of conversion of a substrate into a product comprising: providing a substrate in a carrier; mixing a reactant and a co-factor with the carrier to form a substantially homogeneous mixture of the reactant, the co-factor, and the substrate in the carrier; and reacting the reactant with the substrate in the presence of the co- factor to convert at least a portion of the substrate into the product, wherein the reaction rate of the reactant with the substrate in the presence of the co-factor is different than the reaction rate of the reactant with the substrate in the absence of the co-factor.Type: ApplicationFiled: December 18, 2008Publication date: December 8, 2011Applicant: Georgia Tech Research CorporationInventors: Sujit Banerjee, John T. Reye
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Patent number: 8071350Abstract: In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used as amylases to catalyze the hydrolysis of starch into sugars.Type: GrantFiled: April 7, 2010Date of Patent: December 6, 2011Assignee: Verenium CorporationInventors: Kevin A. Gray, Nahla Aboushadi, James B. Garrett
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Publication number: 20110283421Abstract: The present invention relates to isolated polypeptides having amylolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 2, 2009Publication date: November 17, 2011Applicant: Novozymes, Inc.Inventors: Paul Harris, Mark Wogulis
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Patent number: 8039241Abstract: The present invention relates to an isolated polynucleotide comprising an open reading frame encoding a polypeptide having alpha-amylase activity, the polypeptide selected from the group consisting of: a) a polypeptide comprising an amino acid sequence which has at least 70% identity with amino acids 1 to 450 of SEQ ID NO: 4; b) a polypeptide comprising an amino acid sequence which has at least 70% identity with the polypeptide encoded by the amylase encoding part of the polynucleotide inserted into a plasmid present in the E. coli host deposited under the Budapest Treaty with DSMZ under accession number DSM 15334; c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence which has at least 70% identity with the sequence shown from position 68 to 1417 in SEQ ID NO: 3; and d) a fragment of (a), (b) or (c) that has alpha-amylase activity.Type: GrantFiled: April 6, 2010Date of Patent: October 18, 2011Assignee: I Novozymes A/SInventors: Lan Tang, Wenping Wu, Junxin Duan, Pia Francke Johannesen
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Publication number: 20110250652Abstract: The process of the present invention is based on the microbial production of enzymes from the growth of the fungus Penicillium funiculosum in a suitable culture medium with a cellulosic substrate. The process of the invention comprises in particular the fermentative treatment of a lignocellulosic substrate by means of a specially adapted fungus, for obtaining an enzymatic preparation capable of hydrolysing cellulose and hemicellulose for the production of ethanol.Type: ApplicationFiled: December 22, 2009Publication date: October 13, 2011Applicant: PETROLEO BRASILEIRO S.A.- PETROBRASInventors: Aline Machado de Castro, Lidia Maria Melo Santanna, Nei Pereira Junior, Absai Da Conceicao Gomes, Emerson Pires Menezes, Claudia Julia Groposo Silveira, Danuza Nogueira Moyses, Luiz Fernando Martins Bandeira, Roberto Nobuyuki Maeda
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Patent number: 8034590Abstract: The present invention relates to filamentous fungal host cells and particularly Trichoderma host cells useful for the production of heterologous granular starch hydrolyzing enzymes having glucoamylase activity (GSHE). Further the invention relates to a method for producing a glucose syrup comprising contacting a granular starch slurry obtained from a granular starch substrate simultaneously with an alpha amylase and a GSHE at a temperature equal to or below the gelatinization temperature of the granular starch to obtain a composition of a glucose syrup.Type: GrantFiled: October 19, 2007Date of Patent: October 11, 2011Assignee: Danisco US Inc.Inventors: Toby M. Baldwin, Benjamin S. Bower, Gopal K. Chotani, Nigel Dunn-Coleman, Oreste J. Lantero, Jr., Suzanne E. Lantz, Michael J. Pepsin, Jayarama K. Shetty, Bruce A. Strohm
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Publication number: 20110244499Abstract: In one embodiment the instant invention generally pertains to a method for producing glucose for fermentation. The method comprises first treating a biomass with acid and heat under conditions sufficient to produce a composition mixture comprising cellulose suitable for enzymatic hydrolysis. Next, at least a portion of the cellulose of step (a) is enzymatically hydrolyzed under conditions sufficient to form a composition comprising glucose. The glucose is then fermented. Advantageously, one or more reaction conditions are more efficient because they are selected by first measuring an initial hydrolysis rate of said biomass and then selecting one or more appropriate reaction conditions based upon said initial hydrolysis rate.Type: ApplicationFiled: November 9, 2010Publication date: October 6, 2011Applicants: Georgia Tech Research Corporation, Texaco Inc.Inventors: Matthew James Realff, Ronald W. Smith, Prabuddha Bansal, Andreas Sebastian Bommarius, Melanie Hall, Jay Hyung Lee
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Publication number: 20110244526Abstract: The present invention relates to an alpha-amylase blend, including a B. stearothermophilus alpha-amylase (AmyS) wherein the amino acid at position S242 is substituted and a B. licheniformis alpha-amylase blends for starch liquefaction and saccharification, ethanol production, and a sweetener production.Type: ApplicationFiled: September 11, 2009Publication date: October 6, 2011Applicant: Danisco US Inc.Inventors: Bradley A. Paulson, Vivek Sharma, Jayarama K. Shetty
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Publication number: 20110223639Abstract: The present invention concerns a method of producing glucose from a granular starch substrate comprising, contacting a slurry comprising granular starch obtained from plant material with an alpha-amylase at a temperature below the starch gelatinization temperature of the granular starch to produce oligosaccharides and hydrolyzing the oligosaccharides to produce a mash comprising at least 20% glucose and further comprising fermenting the mash to obtain ethanol.Type: ApplicationFiled: May 23, 2011Publication date: September 15, 2011Applicant: Genencor International, Inc.Inventors: Oreste J. LANTERO, Mian Li, Jayarama K. Shetty
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Patent number: 8017361Abstract: Provided is an enzyme mixture for hydrolyzing a pretreated lignocellulosic feedstock to soluble sugars. The enzyme mixture comprises EG4 at a fractional concentration (fEG4) of about 0.25 to about 0.83 (w/w), Swollenin at a fractional concentration (fSwo1) of about 0 to about 0.66 (w/w), and Cip1 at a fractional concentration (fCip1) of 0 to about 0.33 measured relative to all accessory enzymes present in the enzyme mixture. Also provided are processes for converting a pretreated lignocellulosic feedstock to soluble sugars using the enzyme mixtures, and methods of using and producing such enzyme mixtures.Type: GrantFiled: August 29, 2008Date of Patent: September 13, 2011Assignee: Iogen Energy CorporationInventors: Brian R. Scott, Christopher Hill, John Tomashek, Chengsong Liu
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Publication number: 20110212495Abstract: Methods and apparatus' are disclosed for the simultaneous saccharification and fermentation of biomass providing for the compartmentalization of the saccharification process and the fermentation process resulting in decreased enzymatic end-product inhibition.Type: ApplicationFiled: August 31, 2010Publication date: September 1, 2011Applicant: E.I. DU PONT DE NEMOURS AND COMPANYInventor: BRUCE A. DINER
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Publication number: 20110207182Abstract: The present invention provides for a composition comprising a polypeptide comprising a first amino acid sequence having at least 70% identity with the amino acid sequence of Csac GH5 wherein said first amino acid sequence has a thermostable or thermophilic cellobiohydrolase (CBH) or exoglucanase activity.Type: ApplicationFiled: September 28, 2010Publication date: August 25, 2011Applicant: SANDIA CORPORATIONInventors: Rajat Sapra, Joshua I. Park, Supratim Datta, Blake A. Simmons
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Publication number: 20110201059Abstract: Provided herein are methods for producing fermentable sugar obtained from a plant tissue. The methods include providing transgenic plant material comprising one or more locked carbohydrates and contacting plant material with an enzyme capable of converting the locked carbohydrate into a fermentable sugar. The methods are useful for providing sugar or sugar pre-cursors for several industrial purposes including ethanol production. The invention also encompasses plants and plant parts that produce a lock enzyme to yield a locked carbohydrate, with the consequence of accumulating the locked carbohydrate in the plant. The invention also encompasses providing a key enzyme able to convert locked carbohydrates to fermentable sugars. Key enzymes can be provided by transgenic plants or plant parts, transgenic microbes, transgenic yeast, microbes or yeast.Type: ApplicationFiled: June 11, 2009Publication date: August 18, 2011Inventors: Richard J. Hall, Simon Warner, Rogerio Prata
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Patent number: 7998713Abstract: Ethanol was produced using biocatalysts that are able to ferment sugars derived from treated biomass. Sugars were obtained by pretreating biomass under conditions of high solids and low ammonia concentration, followed by saccharification.Type: GrantFiled: April 12, 2006Date of Patent: August 16, 2011Assignees: E. I. du Pont de Nemours and Company, Alliance for Sustainable Energy LLCInventors: James B. Dunson, Jr., Richard T. Elander, Melvin P. Tucker, III, Susan Marie Hennessey
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Publication number: 20110195918Abstract: Methods for the production of pectin hydrolysis products, the pectin hydrolysis products produced in this manner, as well as their use are described.Type: ApplicationFiled: April 25, 2011Publication date: August 11, 2011Applicant: N.V. NutriciaInventors: Markwart Kunz, Mohammad Munir, Manfred Vogel