Abstract: The present invention provides isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also provides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: A method of digesting a lignocellulosic material is disclosed. In one embodiment, the method comprises the step of exposing the material to an effective amount of Streptomyces sp. ActE secretome such that at least partial lignocellulosic digestion occurs.

Abstract: The present invention relates to an in vitro produced, artificial cellulosome for enzymatic breakdown of resilient substrates. In particular, the present invention provides a complex having an increased activity on resilient substrates, such as crystalline cellulose. The in vitro formed complex comprises a backbone scaffold having at least four binding sites capable of binding the enzyme components, whereby at least two of the binding sites have essentially the same binding specificity; and at least three different enzyme components being randomly bound to the at least four binding sites. Method for preparing the complex and uses of the same for enzymatic breakdown of resilient substrates are also provided.

Abstract: The present invention relates to a method of producing compositions containing galacto-oligosaccharides as well as to galacto-oligosaccharide-containing compositions as such.

Abstract: Systems and methods for cooling and processing materials are disclosed.

Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: The present invention relates to compositions comprising: a polypeptide having cellulolytic enhancing activity and an organic compound. The present invention also relates to methods of using the compositions.

Abstract: The present invention relates to compositions comprising: a polypeptide having cellulolytic enhancing activity and a nitrogen-containing compound. The present invention also relates to methods of using the compositions.

Abstract: Biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce a product or intermediate, e.g., energy, a food, a fuel, or a material.

Abstract: The present disclosure is generally directed to glycosyl hydrolase enzymes, compositions comprising such enzymes, and methods of using the enzymes and compositions, for example for the saccharification of cellulosic and hemicellulosic materials into sugars.

Abstract: The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: The invention relates to a cellulose solution manufacturing method including: performing an ozonation treatment to bring a cellulose-containing material and ozone into contact with each other; and performing an alkali treatment to bring the obtained treated material and an alkali aqueous solution into contact with each other, thereby dissolving at least cellulose in the cellulose-containing material brought into contact with the ozone in the alkali aqueous solution. According to the invention, it is possible to provide a method of manufacturing a cellulose solution in which cellulose can be dissolved in a more simple manner, a method of manufacturing a cellulose precipitate in which cellulose can be recovered from the cellulose solution, and a method of saccharifying cellulose which uses the cellulose precipitate.

Abstract: The invention relates to a method for the synthesis of compounds of general formula (1A) and salts thereof wherein one of the R groups is an ?-sialyl moiety and the other is H, X1 represents a carbohydrate linker, A is a D-glucopyranosyl unit optionally substituted with fucosyl, R1 is a protecting group that is removable by hydrogenolysis, the integer m is 0 or 1, by a transsialidation reaction.

Abstract: Provided are novel ?-neoagarobiose hydrolase and a method for obtaining a monosaccharide using the same.

Abstract: The present invention relates to compositions comprising: a polypeptide having cellulolytic enhancing activity and a quinone compound. The present invention also relates to methods of using the compositions.

Abstract: The present invention relates to compositions comprising: a polypeptide having cellulolytic enhancing activity and a heterocyclic compound. The present invention also relates to methods of using the compositions.

Abstract: Described herein are methods and genetically engineered cells useful for uncapping a mannose-6-phosphate residue on an oligosaccharide.

Abstract: A method for the enzymatic saccharification of a lignocellulosic raw material, including adding a pretreated lignocellulosic raw material as a material suitable for an enzymatic saccharification reaction, together with an electrolyte containing a water-soluble salt, to water that contains a celluolose saccharification enzyme; saccharifying the raw material by an enzymatic saccharification reaction, as a suspension of the raw material having an electrical conductivity adjusted to 5-25 mS/cm; separating and recovering a reaction product and an enzyme-containing solution from the enzymatically saccharified treatment suspension; and recycling the recovered enzyme-containing solution as the enzyme for the enzymatic saccharification step.

Abstract: The present invention relates to compositions comprising: a polypeptide having cellulolytic enhancing activity and a dioxy compound. The present invention also relates to methods of using the compositions.

Abstract: The invention provides compositions and methods for inhibiting the expression of the gene Snl6 in plants. Plants with inhibited expression of Snl6 have use in biofuel production, e.g., by increasing the amount of soluble sugar that can be extracted from the plant.

Abstract: A method for pretreating cellulose for fermentation in an aqueous environment comprises the steps of: grinding a biomass to reduce its particle size, adding water to the ground biomass to create a slurry, soaking the slurry, heating the slurry at a first pressure greater than atmospheric pressure, reducing the pressure of the slurry, reducing the temperature of the slurry, determining whether the enzymes used require preliminary enzymatic hydrolysis, and performing preliminary enzymatic hydrolysis on the slurry if the enzymes used require preliminary enzymatic hydrolysis.

Abstract: Method for processing a lignocellulosic biomass material, comprising (a) a pretreatment process, in which the biomass is prepared for enzymatic hydrolysis, and (b) a subsequent hydrolysis process, in which the pretreated biomass is subjected to enzymatic hydrolysis of its cellulosic components to convert them into sugars, wherein the pretreatment process (a) is carried out in the presence of a tertiary polyamide additive. The additive may be used to improve the efficiency of the hydrolysis process (b). Also provided are processes for the production of a fermentation product from lignocellulosic biomass, and/or for the production of a biofuel or biofuel component.

Abstract: The present disclosure is directed, in a first aspect, to the use of inverting beta-xylosidase enzymes to reduce byproduct formation and increase the yield of fermentation products, as well as, in a second aspect, to the use of retaining beta-xylosidase enzymes to improve production of alkyl-beta-xylopyranoside compounds, in a simultaneous saccharification and fermentation reactions.

Abstract: The present invention provides a process for producing fermentable sugar or a fermentation product from a lignocellulosic feedstock. The process comprises leaching the lignocellulosic feedstock with an aqueous solution to remove at least potassium salts from the lignocellulosic feedstock and without significantly hydrolyzing hemicellulose and cellulose, thereby producing a leached feedstock and leachate. The leachate is removed from the leachate and concentrated. The leached feedstock is hydrolyzed to produce fermentable sugar, which may be fermented to produce a fermentation broth comprising the fermentation product. The concentrated leachate is recirculated to one or more stages in the process involving alkali addition to adjust the pH of a process stream.

Abstract: The embodiments disclosed herein relate to bacterial cell production of triacylglycerides from renewable biomass resources.

Abstract: A process for production of fermentable sugars from biomass using multi-enzyme multi-step system is provided herein. The process disclosed in the present invention provides high yielded sugars in less time period. The multi-enzyme system disclosed in the present invention converts celluloses, hemicelluloses and/or mixture thereof to fermentable sugar with higher efficiency and better economics than the process known in the prior art. Cellulose and hemicelluloses fractions derived from natural sources such as any lignocellulosic biomass are saccharified in a shortened time with higher conversion rates of intermediates with modified enzymatic compositions/groups of the Multi-enzyme system to enhance the rate thus providing an economical cellulose and hemicellulose saccharification process.

Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: Plant biomass is immersed in a solution that contains a polar solvent and an imidazolium salt that has a melting point of at least 100° C. As a result, the cellulose and hemicellulose present in the plant biomass are relaxed (decrystallized and depolymerized) and brought into an easy-to-degrade state. Reacting the immersed plant biomass with a cellulase produces saccharide at a high conversion efficiency.

Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: A method is provided for producing an improved pretreated biomass product for use in saccharification followed by fermentation to produce a target chemical that includes removal of saccharification and or fermentation inhibitors from the pretreated biomass product. Specifically, the pretreated biomass product derived from using the present method has fewer inhibitors of saccharification and/or fermentation without a loss in sugar content.

Abstract: An aqueous composition which is anionic in nature, includes at least one soluble gelatinized anionic starch and at least one insoluble anionic starch. A method of using the composition, in particular for the manufacture of a paper or of a flat board, and the paper or the flat board obtained therefrom, along with various industrial uses such as water treatment or the production of cosmetic products, are also described.

Abstract: The present invention relates to a method for preparing a processed ginseng or processed ginseng extract. Specifically, the invention relates to a method for preparing a processed ginseng or processed ginseng extract having increased ginsenoside contents. More specifically, the invention relates to a method of preparing a novel processed ginseng or processed ginseng extract having increased ginsenoside contents by preparing saponinase, fermenting ginseng or red ginseng with the prepared saponinase and hydrolyzing the fermented ginseng or red ginseng with an organic acid and to an anticancer supplement composition or pharmaceutical composition comprising the processed ginseng or processed ginseng extract prepared thereby.

Abstract: A saccharide-solution production method using a biomass material, including feeding a biomass material containing cellulose, hemicellulose, and lignin under a normal pressure to put it under an increased pressure; hydrothermally decomposing the biomass material using pressurized hot water by a hydrothermal decomposition unit; and dissolving a lignin component and a hemicellulose component in the pressurized hot water; thereafter, adding a biomass solid discharged from the hydrothermal decomposition unit to a slurrying vessel containing water injected therein and communicating with the hydrothermal decomposition unit so as to obtain a slurried biomass solid; then, removing water from the slurried biomass solid; and thereafter, performing enzymatic saccharification of the water-removed biomass solid to produce a saccharide solution.

Abstract: The invention discloses cellulase enzymes with optimized properties for processing of cellulose- and lignocellulose-containing substrates. In particular, cellobiohydrolase enzymes with preferred characteristics are disclosed. The present invention provides fusion, insertion, deletion and/or substitution variants of such enzymes. Enzyme variants have enhanced thermostability, proteolytic stability, specific activity and/or stability at extreme pH. Nucleic acid molecules encoding said enzymes, a composition comprising said enzymes, a method for preparation, and the use for cellulose processing and/or for the production of biofuels are disclosed.

Abstract: It is intended to provide a method for treating lignocellulosic biomass, which can reliably show the completion of the course by which pretreated lignocellulosic biomass is rendered flowable and thus transportable. The method for treating lignocellulosic biomass comprises a first saccharification step of saccharifying pretreated lignocellulosic biomass with stirring using a stirring unit 7 to obtain a first saccharification product that is flowable and thus transportable and a second saccharification step of obtaining a second saccharification product from the first saccharification product, wherein the rate of change dW/dt of power W required for the stirring in the stirring unit 7 versus stirring time t falls below a predetermined value, after which the first saccharification product is transported under non-air-contact conditions from the first saccharification step to the second saccharification step.

Abstract: The present invention relates to hybrid polypeptides having a first amino acid sequence having endo-amylase activity and a second amino acid sequence having carbohydrate binding activity. The present invention also relates to the use of the hybrid polypeptides in starch processing and baking.

Abstract: Described are compositions and methods relating to starch processing without a phytase pretreatment step and without adjustment of the slurry pH adjustment.

Abstract: The present disclosure is directed to the use of mammalian promoters to drive recombinant expression in filamentous fungal cells. In certain aspects, the present disclosure provides an expression cassette useful for the expression of polypeptide in filamentous fungal cells. Also provided herein, are vectors and recombinant filamentous fungal cells comprising the expression cassettes of the present disclosure, and methods of making and using the same for recombinant polypeptide expression.

Abstract: The present invention relates to polypeptides having xylanase activity and nucleic acid sequences encoding such polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides. One specific application of the xylanase is the selective hydrolysis of pentose sugar components of hemicellulose-containing plant biomass. The nucleotide sequence may be used for the production of the xylanase or optimized mutants thereof.

Abstract: The present disclosure is directed to the use of plant promoters to drive recombinant expression in filamentous fungal cells. In certain aspects, the present disclosure provides an expression cassette useful for the expression of polypeptide in filamentous fungal cells. Also provided herein, are vectors and recombinant filamentous fungal cells comprising the expression cassettes of the present disclosure, and methods of making and using the same for recombinant polypeptide expression.

Abstract: The invention provides methods for treatment of feedstock to reduce the relative viscosity and promote release of fermentable sugars.

Abstract: Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy or sugary materials, to produce ethanol and/or butanol, e.g., by fermentation.

Abstract: The invention relates to a polypeptide comprising the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 70% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 70% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional polypeptide and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.

Abstract: The invention provides variants of the Azospirillum irakense CelA ?-glucosidase that have improve ?-glucosidase activity, particularly improved thermoactivity, compared to the wild type enzyme. The invention further provides related polynucleotides, vectors, host cell, and methods for making and using the variants.

Abstract: The present invention provides fungal proteases and improved fungal strains that are deficient in protease production.

Abstract: The present invention relates to isolated polypeptides having isoamylase activity derived from Dyella japonica and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. The invention also relates to the use of said polypeptide having isoamylase activity for producing glucose syrup, fructose syrup, maltose syrup or maltitol.

Abstract: A process for the production of carbohydrate cleavage products, characterized by a combination of the measures that a lignocellulosic material is treated with an aqueous solution containing an alcohol, in particular a C1-4-alcohol or a phenol, and having a pH-value of between 11.0 and 14.0 in order to cleave lignocellulose and separate cleavage products from the material, whereby a material enriched with cellulose and hemicellulose is obtained, and the obtained material enriched with cellulose and hemicellulose is treated with at least one carbohydrate-cleaving enzyme in order to obtain the carbohydrate cleavage products.

Abstract: Nucleic acids encoding glycosidases useful for the hydrolysis of cellulose and hemicellulose obtained from ciliates residing in a bovine rumen are provided. Also provided recombinant nucleic acids encoding the glycosidases, transformed cells comprising the same and related methods of using the transformed cells and glycosidases to degrade cellulose and/or hemicellulose.

Abstract: A process for production of fermentable sugars from biomass using multi-enzyme multi-step system is provided herein. The process disclosed in the present invention provides high yielded sugars in less time period. The multi-enzyme system disclosed in the present invention converts celluloses, hemicelluloses and/or mixture thereof to fermentable sugar with higher efficiency and better economics than the process known in the prior art. Cellulose and hemicelluloses fractions derived from natural sources such as any lignocellulosic biomass are saccharified in a shortened time with higher conversion rates of intermediates with modified enzymatic compositions/groups of the Multi-enzyme system to enhance the rate thus providing an economical cellulose and hemicellulose saccharification process.