Hydroxyl Containing Patents (Class 436/131)
  • Patent number: 8513023
    Abstract: A method of determining the functional activity of the MRP2 and/or MRP3 efflux pathway of a human or animal subject was disclosed. The method comprises (i) determining the level of a bile acid derivative in the blood of said human or animal subject at a predetermined time interval after introducing an amount of the bile acid derivative into the subject, and (ii) using the determination obtained in step (i) to indicate the functional activity of the MRP2 and/or MRP3 efflux pathway of the subject.
    Type: Grant
    Filed: October 25, 2012
    Date of Patent: August 20, 2013
    Assignee: Norgine BV
    Inventors: Hans-Jürgen Gruss, Ronald Oude Elferink, Dirk De Waart, Bruno Stieger
  • Patent number: 8501398
    Abstract: The invention provides a method of determining the amount of an analyte having an oxidation potential, for a one electron oxidation process, of about +0.10 to about +1.20 volts at pH 7, relative to the normal hydrogen electrode at 298K, said method comprising measuring the emission intensity or emission lifetime, at two or more wavelengths, from a sample comprising said analyte and two or more different macrocyclic lanthanide (III) complexes, wherein each of said macrocyclic lanthanide (III) complexes comprises a different lanthanide ion but the same macrocyclic ligand, and using a ratio of emission intensities or emission lifetimes measured at two different wavelengths to calculate the amount of analyte in said sample.
    Type: Grant
    Filed: July 11, 2007
    Date of Patent: August 6, 2013
    Assignee: University of Durham
    Inventors: David Parker, Robert Anthony Poole, Filip Kielar
  • Patent number: 8476075
    Abstract: A method of estimating the amount of unreacted starting materials (glycerides, methyl esters, etc.) and the composition of a biodiesel using TLC in conjunction with a lipophilic dye, Nile Red is described herein. The dye based TLC method of the present invention is convenient and provides significant advantages over existing methods for estimating the purity of a biodiesel composition.
    Type: Grant
    Filed: April 6, 2010
    Date of Patent: July 2, 2013
    Assignee: Board of Regents, The University of Texas System
    Inventors: Eva Deemer, Wen Yee Lee, Xiomara Carolina Chianelli
  • Patent number: 8470602
    Abstract: Disclosed are methods and systems for the analysis of delta 5 steroid compounds in a sample using supported liquid extraction and liquid chromatography/mass spectrometry.
    Type: Grant
    Filed: February 7, 2012
    Date of Patent: June 25, 2013
    Assignee: Laboratory Corporation of America Holdings
    Inventors: Donald Walt Chandler, Russell Philip Grant, Samuel Meares, Patricia Holland
  • Publication number: 20130143329
    Abstract: Provided are methods of detecting the presence or amount of a dihydroxyvitamin D metabolite in a sample using mass spectrometry. The methods generally comprise ionizing a dihydorxyvitamin D metabolite in a sample and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample. In certain preferred embodiments the methods include immunopurifying the dihydroxyvitamin D metabolites prior to mass spectrometry. Also provided are methods to detect the presence or amount of two or more dihydroxyvitamin D metabolites in a single assay.
    Type: Application
    Filed: January 28, 2013
    Publication date: June 6, 2013
    Inventors: Brett Holmquist, Nigel J. Clarke, Anne Caston-Balderrama, Richard E. Reitz
  • Publication number: 20130137128
    Abstract: A method of monitoring water-soluble treatment chemicals in a fluid that is immiscible with water and which may or may not contain some aqueous fluid, the method using at least one reagent that produces an optically detectable product, the detection step can take place without separation of the aqueous phase containing the treatment chemicals from the fluid immiscible with water.
    Type: Application
    Filed: May 27, 2011
    Publication date: May 30, 2013
    Applicant: LUX INNOVATE LIMITED
    Inventors: Fiona MacKay, Emma Perfect, Catherine Rowley-Williams, Anne-Marie Fuller
  • Patent number: 8431411
    Abstract: Provided are methods of detecting the presence or amount of a vitamin D metabolite in a sample using mass spectrometry. The methods generally comprise ionizing a vitamin D metabolite in a sample and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample. Also provided are methods to detect the presence or amount of two or more vitamin D metabolites in a single assay.
    Type: Grant
    Filed: December 15, 2011
    Date of Patent: April 30, 2013
    Assignee: Quest Diagnostics Investments Incorporated
    Inventors: Nigel Clarke, Brett Holmquist, Gloria Kwang-Ja Lee, Richard E. Reitz
  • Patent number: 8415162
    Abstract: Described herein are methods for determining an amount of an analyte in a test sample. The methods involve preparing a calibration curve using standard samples containing an isotopically-labeled standard in a biological matrix.
    Type: Grant
    Filed: August 3, 2012
    Date of Patent: April 9, 2013
    Assignee: PerkinElmer Health Sciences, Inc.
    Inventors: Blas Cerda, Alex Cherkasskiy
  • Patent number: 8415166
    Abstract: The present invention provides a device for the detection of a peroxide-based explosive, in particular, triacetone triperoxide (TATP), which is based on a molecular controlled semiconductor resistor (MOCSER) and composed of at least one insulating or semi-insulating layer, at least one conducting semiconductor layer, two conducting pads and a layer of multifunctional organic molecules capable of adsorbing molecules of the peroxide-based explosive. The invention further provides an array of semiconductor devices for the selective detection of a peroxide-based explosive, as well as a method for the selective detection of vapors of a peroxide-based explosive in a gaseous mixture using this array.
    Type: Grant
    Filed: May 12, 2009
    Date of Patent: April 9, 2013
    Assignee: Yeda Research and Development Co. Ltd.
    Inventors: Ron Naaman, Eyal Capua, Roberto Cao
  • Patent number: 8409865
    Abstract: Embodiments of the present invention are directed to methods and kits for determining the presence or absence, and the amount if present, of vitamin D analogs in samples. One embodiment of the present method for detecting the presence or absence of vitamin D analogs in a sample comprises the steps of adding an effective amount of a multiple charge cationic agent to the sample to form a cationic treated sample. The effective amount of a multiple charge cationic agent enhances the signal from vitamin D analogs upon analysis by mass spectroscopy.
    Type: Grant
    Filed: February 20, 2009
    Date of Patent: April 2, 2013
    Assignee: Waters Technologies Corp.
    Inventors: Lisa Jane Calton, Kendon Stuart Graham
  • Publication number: 20130078729
    Abstract: A reagent composition for releasing vitamin D compounds bound to vitamin D-binding protein, an in vitro method for the detection of a vitamin D compound in which the vitamin D compound is released from vitamin D-binding protein by the use of this reagent composition and the reagent mixture obtained in this manner. The use of the disclosed reagent composition to release vitamin D compounds as well as a kit for detecting a vitamin D compound which contains the reagent composition for releasing vitamin D compounds in addition to common detecting reagents.
    Type: Application
    Filed: November 19, 2012
    Publication date: March 28, 2013
    Applicant: ROCHE DIAGNOSTICS OPERATIONS, INC.
    Inventor: ROCHE DIAGNOSTICS OPERATIONS, INC.
  • Patent number: 8383064
    Abstract: A test module for performing a genetic diagnostic assay, the test module having an outer casing dimensioned for hand-held portability, the outer casing having a receptacle for a biological sample containing target nucleic acid sequences, an array of chambers containing probes for hybridization with the target nucleic acid sequences to form probe-target hybrids, a flow-path extending from the inlet to the probes, and, a reagent reservoir containing a reagent for addition to the sample in the flow-path upstream of the probes, wherein, each of the chambers contains less than 270 picograms of probe and the reagent reservoir has a volume less than 1000,000,000 cubic microns.
    Type: Grant
    Filed: June 1, 2011
    Date of Patent: February 26, 2013
    Assignee: Silverbrook Research Pty Ltd
    Inventors: Mehdi Azimi, Kia Silverbrook
  • Publication number: 20130040398
    Abstract: Methods for determining the amount of vitamin D compounds in a sample are provided. The methods can employ LC-MS/MS techniques and optionally the use of deuterated internal standards. Methods for diagnosing vitamin D deficiencies are also provided.
    Type: Application
    Filed: October 19, 2012
    Publication date: February 14, 2013
    Applicant: MAYO FOUNDATION FOR MEDICAL EDUCATION AND RESEARCH
    Inventor: Mayo Foundation for Medical Education and Rese
  • Publication number: 20130034909
    Abstract: Provided is a diagnosis method for an oil-filled electrical apparatus, which is for evaluating the degree of danger of copper sulfide being generated within the oil-filled electrical apparatus, comprising: a first step for detecting a specific compound contained within insulating oil inside the oil-filled electrical apparatus; a second step for evaluating the possibility of copper sulfide being generated inside the oil-filled electrical apparatus, on the basis of the result detected by the first step; and a third step for diagnosing the degree of danger of a malfunction occurring in the oil-filled electrical apparatus, on the basis of the evaluation result obtained in the second step. The specific compound contains dibenzyl disulfide and/or a reaction product of a radical resulting from dibenzyl disulfide, and di-tert-butyl-p-cresol and/or a reaction product of a radical resulting from di-tert-butyl-p-cresol, or, di-tert-butyl-phenol and/or a reaction product of a radical resulting from di-tert-butyl-phenol.
    Type: Application
    Filed: May 10, 2011
    Publication date: February 7, 2013
    Applicant: Mitsubishi Electric Corporation
    Inventors: Satoru Toyama, Junji Tanimura, Fukutaro Kato, Tsuyoshi Amimoto, Eiichi Nagao, Takeshi Kawashima, Noboru Hosokawa
  • Publication number: 20130011926
    Abstract: A vapochromic gold-copper complex [AuL2(Cu(Y)n)2](X)3 exhibiting luminescence is provided, where L is an N-heterocyclic carbene; Y is a heteroatom-containing ligand; X is an anion, and n is an integer having a value of 1 or 2, and solvates thereof. A reaction of [AuL2(Cu(Y)n)2](X)3 with water vapor or an organic compound vapor, for example, affords a modified complex that yields a change in luminescence color under UV excitation. These tricationic vapochromic materials exhibit large changes in the emission through ligand substitution reactions between the solid complex and vapors, which permit use in luminescent vapochromic sensors.
    Type: Application
    Filed: June 29, 2012
    Publication date: January 10, 2013
    Applicant: Board of Regents of the Nevada System of Higher Education, on behalf of the University of Nevada,
    Inventors: Vincent J. Catalano, Christoph E. Strasser
  • Patent number: 8318504
    Abstract: In one aspect a method of determining the functional activity of the MRP2 and/or MRP3 efflux pathway of a human or animal subject, comprises: (i) determining the level of a bile acid derivative in the blood of said human or animal subject at a predetermined time interval after introducing an amount of the bile acid derivative into the subject; and (ii) using the determination obtained in step (i) to indicate the functional activity of the MRP2 and/or MRP3 efflux pathway of the subject.
    Type: Grant
    Filed: May 14, 2009
    Date of Patent: November 27, 2012
    Assignee: Norgine BV
    Inventors: Hans-Jürgen Gruss, Ronald Oude Elferink, Dirk de Waart, Bruno Stieger
  • Publication number: 20120295361
    Abstract: Described herein are methods for determining an amount of an analyte in a test sample. The methods involve preparing a calibration curve using standard samples containing an isotopically-labeled standard in a biological matrix.
    Type: Application
    Filed: August 3, 2012
    Publication date: November 22, 2012
    Inventors: Blas Cerda, Alex Cherkasskiy
  • Patent number: 8313697
    Abstract: The present invention relates to a cartridge 1 including a plurality of analyzing tools 3 arranged lined in a plane direction and a case 2 for accommodating the plurality of analyzing tools 3, and being configured to take out the analyzing tool 3 one at a time from the case 2. The plurality of analyzing tools 3 further include engagement means 32, 33 for restricting the analyzing tools 3 adjacent to each other in the plane direction and allowing removable attachment in a thickness direction D1, D2 of the analyzing tool 3. The present invention further relates to an analyzer and an analyzing system for analyzing a sample using the cartridge 1.
    Type: Grant
    Filed: November 6, 2007
    Date of Patent: November 20, 2012
    Assignee: Arkray, Inc.
    Inventor: Yoshiharu Sato
  • Publication number: 20120244627
    Abstract: Provided are methods of detecting the presence or amount of a dihydroxyvitamin D metabolite in a sample using mass spectrometry. The methods generally comprise associating an amine with a dihydroxyvitamin D metabolite in a sample, ionizing the adduct, and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample.
    Type: Application
    Filed: October 19, 2010
    Publication date: September 27, 2012
    Applicant: Georgetown University
    Inventor: Steven J. Soldin
  • Publication number: 20120231549
    Abstract: Implementations and techniques for sensing hydroxyl radicals in ozone washing systems are generally disclosed.
    Type: Application
    Filed: March 9, 2011
    Publication date: September 13, 2012
    Applicant: EMPIRE TECHNOLOGY DEVELOPMENT LLC
    Inventor: Seth Miller
  • Patent number: 8257977
    Abstract: Described herein are methods for determining an amount of an analyte in a test sample. The methods involve preparing a calibration curve using standard samples containing an isotopically-labeled standard in a biological matrix.
    Type: Grant
    Filed: July 26, 2010
    Date of Patent: September 4, 2012
    Assignee: PerkinElmer Health Sciences, Inc.
    Inventors: Blas Cerda, Alex Cherkasskiy
  • Patent number: 8258081
    Abstract: Compositions and methods for inhibiting the sprouting of potato tubers are provided. The compositions comprise C3 to C14 aliphatic aldehydes and ketones, and/or C3 to C7 primary and secondary aliphatic alcohols.
    Type: Grant
    Filed: August 6, 2008
    Date of Patent: September 4, 2012
    Assignee: Washington State University Research Foundation
    Inventors: Norman Richard Knowles, Lisa O'Rear Knowles
  • Publication number: 20120214244
    Abstract: Provided is a method for quantitative analysis of carotenoids such as ?-cryptoxanthin contained in a sample such as citrus fruit or the like which enables simple and accurate quantitative analysis. An internal standard such as lycopene or retinal is used, whereby work is facilitated and an error of a measured value is reduced. Further, a dehydrating agent is added during saponification reaction in the preparation of a sample for analysis, whereby a deviation of a measured value can be reduced.
    Type: Application
    Filed: October 15, 2010
    Publication date: August 23, 2012
    Applicant: SUNTORY HOLDINGS LIMITED
    Inventor: Naoki Kasajima
  • Publication number: 20120190121
    Abstract: Provided are methods of detecting the presence or amount of a dihydroxyvitamin D metabolite in a sample using mass spectrometry. The methods generally comprise ionizing a dihydorxyvitamin D metabolite in a sample and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample. In certain preferred embodiments the methods include immunopurifying the dihydroxyvitamin D metabolites prior to mass spectrometry. Also provided are methods to detect the presence or amount of two or more dihydroxyvitamin D metabolites in a single assay.
    Type: Application
    Filed: March 30, 2012
    Publication date: July 26, 2012
    Inventors: Brett Holmquist, Nigel J. Clarke, Anne Caston-Balderrama, Richard E. Reitz
  • Publication number: 20120142545
    Abstract: A method for the equilibration of enriched isotope species and natural isotope species prior to mass spectrometric analysis using solid phase and/or microwave isotope ratio equilibration and measurement.
    Type: Application
    Filed: December 7, 2007
    Publication date: June 7, 2012
    Inventors: Howard M. Kingston, Mizanur Rahman, David Lineman, Mehmet Pamukcu
  • Patent number: 8193001
    Abstract: The present invention concerns methods and compositions to prepare biological samples to preserve the macromolecules in the samples. Embodiments of the invention concern the use of a soak solution that contains one or more water-miscible solvents. A sample is incubated with the soak solution to the point of saturation at a temperature above the melting temperature of the water-miscible solvent but below 0° C. The use of methods and compositions of the invention allow for subsequent preparation or analysis of the samples.
    Type: Grant
    Filed: February 23, 2009
    Date of Patent: June 5, 2012
    Assignee: Applied Biosystems, LLC
    Inventors: Matthew M. Winkler, Richard C. Conrad
  • Patent number: 8187891
    Abstract: The present invention provides a method for detection or determination of vitamin C by luminescence that employs a redox-responsive fluorogenic probe consisting of porphyrin or phthalocyanine with a nitroxide radical(s), or that employs a liposome preparation comprising the said redox-responsive fluorogenic probe. The present method can overcome the problems of prior art methods, and in particular, are applicable for bioimaging to allow elucidation of the behavior of vitamin C in vivo.
    Type: Grant
    Filed: March 17, 2010
    Date of Patent: May 29, 2012
    Assignee: The University of Tokyo
    Inventors: Kazuyuki Ishii, Kensuke Kubo, Kikuo Komori, Yasuyuki Sakai
  • Publication number: 20120115240
    Abstract: Methane gas in a ruminant exhalation may be oxidized to reduce the amount of methane gas output by the ruminant.
    Type: Application
    Filed: November 10, 2010
    Publication date: May 10, 2012
    Inventors: Kenneth G. Caldeira, Roderick A. Hyde, Muriel Y. Ishikawa, Jordin T. Kare, John Latham, Nathan P. Myhrvold, Stephen H. Salter, Clarence T. Tegreene, David B. Tuckerman, Thomas Allan Weaver, Charles Whitmer, Lowell L. Wood, JR., Victoria Y.H. Wood, JR.
  • Patent number: 8158435
    Abstract: Embodiments of the present invention provide for detecting and/or screening for conditions associated with altered sterols by derivatization of sterols to provide suitable sensitivity and selectivity of detection using tandem mass spectrometry with electrospray ionization. Such testing may be performed to detect and/or screen for conditions such as Smith-Lemli-Opitz syndrome, familial hypercholesterolaemia, and cerebrotendinous xanthomatosis, or certain bile acid disorders.
    Type: Grant
    Filed: May 2, 2008
    Date of Patent: April 17, 2012
    Assignee: Oregon Health & Science University
    Inventors: Andrea DeBarber, Robert D. Steiner
  • Patent number: 8137978
    Abstract: A diagnostic method using biomarkers to predict future adverse coronary events is provided. More particularly, the present invention is directed to diagnostic tests for characterizing an individual's risk of developing or having cardiovascular disease. In certain embodiments, the method of the present invention quantitates the presence of elevated levels of chlorinated lipids derived from myeloperoxidase as a prognostic indicator of future adverse coronary events.
    Type: Grant
    Filed: October 16, 2009
    Date of Patent: March 20, 2012
    Assignee: Saint Louis University
    Inventor: David A. Ford
  • Patent number: 8114674
    Abstract: The present invention relates to a method for determining whether or not a mammal is affected with a lung cancer. The method for determining whether or not a mammal is affected with a lung cancer of the present invention includes the steps of: (a) detecting whether or not anthranilic acid is contained in the urine excreted from the mammal; and (b) determining that the mammal is affected with a lung cancer when the anthranilic acid is detected as being contained in the urine.
    Type: Grant
    Filed: April 22, 2010
    Date of Patent: February 14, 2012
    Assignee: Panasonic Corporation
    Inventors: Yuki Sugibayashi, Yosuke Hanai
  • Patent number: 8101427
    Abstract: Provided are methods of detecting the presence or amount of a vitamin D metabolite in a sample using mass spectrometry. The methods generally comprise ionizing a vitamin D metabolite in a sample and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample. Also provided are methods to detect the presence or amount of two or more vitamin D metabolites in a single assay.
    Type: Grant
    Filed: May 25, 2011
    Date of Patent: January 24, 2012
    Assignee: Quest Diagnostics Investments Incorporated
    Inventors: Nigel Clarke, Brett Holmquist, Kwang-Ja Lee, Richard E. Reitz
  • Patent number: 8082109
    Abstract: The invention relates to computational methods, systems and apparatus useful in the analysis of sets of biomolecules in an accessible body fluid or tissue sample from a patient, which biomolecules collectively or individually are candidates to serve as biomarkers, i.e., biomolecules which together or individually upon detection or change are indicative that the patient is in some biological state, such as a diseased state. The methods permit one to examine such potential biological markers to determine whether each one is indeed present as a consequence of the biological state, or as an artifact of the biomarker search protocol.
    Type: Grant
    Filed: August 28, 2008
    Date of Patent: December 20, 2011
    Assignee: Selventa, Inc.
    Inventors: William M. Ladd, Keith O. Elliston, Joseph J. Loureiro
  • Publication number: 20110300635
    Abstract: Articles and methods for the detection and/or quantification of organic analytes are described. The invention provides a chemical indicator test strip, comprising: a substrate; and a coating comprising a leuco dye complex on the substrate, the coating being insoluble in water and reactive with an organic analyte, the coating derived from a solution of leuco dye and developing agent. In another aspect, the coating further comprises adjuvant, in the form of a water-insoluble, polar, hydrophobic, aprotic material that extends the lower limit of detection for the analyte. In still another aspect, the present invention provides a method of using a chemical indicator test strip, comprising: (a) exposing the chemical indicator test strip to a sample comprising an organic analyte; (b) measuring a color change on the chemical indicator test strip following the exposing step; and (c) correlating the color change with the concentration of the analyte in the sample.
    Type: Application
    Filed: August 16, 2011
    Publication date: December 8, 2011
    Inventor: Vinod P. Menon
  • Patent number: 8071387
    Abstract: Electrodeposition baths, systems and methods are provided. In some embodiments, the baths, systems and methods are used to deposit metal alloy coatings.
    Type: Grant
    Filed: May 27, 2011
    Date of Patent: December 6, 2011
    Assignee: Xtalic Corporation
    Inventors: Alan C. Lund, Nazila Dadvand, John Cahalen, Daniel J. Montville
  • Publication number: 20110212534
    Abstract: Methods for determining the amount of DHVD2 and/or DHVD3 in a sample are provided. The methods can employ LC-MS/MS techniques coupled with sample affinity purification and derivatization steps. Methods for diagnosing vitamin D deficiencies are also provided.
    Type: Application
    Filed: August 11, 2009
    Publication date: September 1, 2011
    Inventors: Robert L. Taylor, Ravinder J. Singh, Kendall W. Cradic, James L. Bruton, JR., Brian C. Netzel
  • Patent number: 8003400
    Abstract: The present invention relates to a method of measuring a vitamin D metabolite in a sample, the method comprising the steps of (a) treating said sample with a vitamin D metabolite releasing reagent under conditions appropriate to release a vitamin D metabolite from vitamin D-binding protein and not to cause protein precipitation, (b) subjecting the treated sample obtained in step (a) to a chromatographic separation, and (c) measuring a vitamin D metabolite during or after said chromatographic separation. The present invention also relates to methods for determining the vitamin D status of a subject, for use in the diagnosis of disease, and to agents and kits for use in performing the methods of the invention.
    Type: Grant
    Filed: December 3, 2008
    Date of Patent: August 23, 2011
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Uwe Kobold, Thomas Duelffer, Michael Grol, Rupert Herrmann, Herbert Von der Eltz, Leopold Von Proff
  • Patent number: 8003393
    Abstract: The present disclosure relates to a method for assessing if a patient is affected with a lung cancer. The method includes analyzing the presence and amount of ?-citronellol contained in the urine excreted from the patient and determining if the presence and amount of ?-citronellol is indicative of lung cancer.
    Type: Grant
    Filed: December 2, 2010
    Date of Patent: August 23, 2011
    Assignee: Panasonic Corporation
    Inventors: Yosuke Hanai, Hiroaki Oka
  • Publication number: 20110195513
    Abstract: Embodiments of the present invention are directed to methods and kits for determining the presence or absence, and the amount if present, of vitamin D analogs in samples. One embodiment of the present method for detecting the presence or absence of vitamin D analogs in a sample comprises the steps of adding an effective amount of a multiple charge cationic agent to the sample to form a cationic treated sample. The effective amount of a multiple charge cationic agent enhances the signal from vitamin D analogs upon analysis by mass spectroscopy.
    Type: Application
    Filed: February 20, 2009
    Publication date: August 11, 2011
    Applicant: WATERS TECHNOLOGIES CORPORATION
    Inventors: Lisa Jane Calton, Kendon Stuart Graham
  • Patent number: 7989212
    Abstract: A detection method of blood plasma schizadrin B of dissipating blood stasis botanical is disclosed. The method includes: (1) extracting schizadrin B from plasma of mammalian administered dissipating blood stasis botanical by ethyl acetate with the volume ratio 1:4, whirling 3-5 mins, centrifugating at 9600 rpm for 10 mins, drying and enriching the upper layer at 25-30° C., and redissolving with mobile phase; (2) UPLC/MS measuring: UPLC condition: chromatographic column: Acquity UPLC BEH C18, 2.1?100 mm, mobile phase A: water-acetonitrile-formic acid 95:5:0.1 v/v/v, mobile phase B: acetonitrile-formic acid 100:0.1 v/v; MS condition: electric spraying ion source (ESI), detecting with positive ion mode, scanning at the range of m/z 150-1200. The method can be used for pharmacokinetics study of schizadrin B in dissipating blood stasis botanical.
    Type: Grant
    Filed: April 28, 2008
    Date of Patent: August 2, 2011
    Assignee: Shanghai Sundise
    Inventors: Yueming Ma, Rong Shi, Yongyu Zhang
  • Publication number: 20110183429
    Abstract: Quantification of vitamin D2, vitamin D3, and the monohydroxy and diihydroxy metabolites of vitamin D2 and vitamin D3, can comprise labeling analytes with mass spectrometry (MS) tagging reagents and performing LC-MSMS analysis of the labeled analytes. The labeled analytes can include a labeled standard and can have distinct retention times on a reversed phase column, as well as distinct masses. Under high energy collisions, reporter groups can be generated. The intensity or the peak area detected for each reporter group can be used for quantitation. In some embodiments, a one-step tagging reagent is used that is a dienophile-containing, labeled Diels Alder reagent.
    Type: Application
    Filed: January 25, 2011
    Publication date: July 28, 2011
    Inventors: Subhakar DEY, Sasi PILLAI, Brian WILLIAMSON, Subhasish PURKAYASTHA
  • Patent number: 7977099
    Abstract: The present invention provides a low-cost, easy-to-use, and accurate method and apparatus for determining alcohol content in a test sample, such as wine. The method for alcohol content determination includes the following steps. A known quantity of a test sample is provided. A measurement of electrolytic conductivity EC0 of the test sample is taken. Next, a known quantity of conductive additive with known composition is added to the test sample to produce a first solution. A measurement of Electrolytic conductivity EC1 is taken for the first solution. Data representing predetermined values for EC0 and EC1 with corresponding known alcohol concentrations is provided for comparison. The electrolytic conductivity values for EC0 and EC1 are cross-referenced against the known data to determine a value for the alcohol concentration of the test sample. In addition, the values for EC0 and EC1 are adjusted for temperature and other variables.
    Type: Grant
    Filed: August 15, 2008
    Date of Patent: July 12, 2011
    Assignee: Hanna Instruments, Inc.
    Inventors: Oscar Nardo, Martino Nardo, Piero Franco
  • Patent number: 7972868
    Abstract: Provided are methods of detecting the presence or amount of a dihydroxyvitamin in D metabolite in a sample using mass spectrometry. The methods generally comprise ionizing a dihydroxyvitamin D metabolite in a sample and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample. In certain preferred embodiments the methods include immunopurifying the dihydroxyvitamin D metabolites prior to mass spectrometry. Also provided are methods to detect the presence or amount of two or more dihydroxyvitamin D metabolites in a single assay.
    Type: Grant
    Filed: November 28, 2007
    Date of Patent: July 5, 2011
    Assignee: Quest Diagnostics Investments Incorporated
    Inventors: Brett Holmquist, Nigel J. Clarke, Anne Caston-Balderrama, Richard E. Reitz
  • Patent number: 7972867
    Abstract: Provided are methods of detecting the presence or amount of a vitamin D metabolite in a sample using mass spectrometry. The methods generally comprise ionizing a vitamin D metabolite in a sample and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample. Also provided are methods to detect the presence or amount of two or more vitamin D metabolites in a single assay.
    Type: Grant
    Filed: March 21, 2006
    Date of Patent: July 5, 2011
    Assignee: Quest Diagnostics Investments Incorporated
    Inventors: Nigel Clarke, Brett Holmquist, Gloria Kwangja Lee, Richard E. Reitz
  • Patent number: 7951600
    Abstract: Electrodeposition baths, systems and methods are provided. In some embodiments, the baths, systems and methods are used to deposit metal alloy coatings.
    Type: Grant
    Filed: November 7, 2008
    Date of Patent: May 31, 2011
    Assignee: Xtalic Corporation
    Inventors: Alan C. Lund, Nazila Dadvand, John Cahalen, Daniel J. Montville
  • Patent number: 7939340
    Abstract: The invention relates to compositions, kits and methods used in hydroxyl radical detection. In some embodiments, the invention relates to compositions comprising a dye preferably methylene blue immobilized on a substrate. In additional embodiments, the invention relates methods of correlating color changes of a dye to the presence or absence of hydroxyl radicals. In some embodiments, the invention relates to a methylene blue dye containing test strip and its use in a method for detecting the presence of hydroxyl radicals.
    Type: Grant
    Filed: June 28, 2006
    Date of Patent: May 10, 2011
    Assignee: Board Of Trustees Of Michigan State University
    Inventors: Andrea Yuki Satoh, Susan J. Masten, James Trosko
  • Publication number: 20110097733
    Abstract: The invention concerns a process for the production of a hybridoma, and of a monoclonal antibody or fragments thereof able to recognize 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3.
    Type: Application
    Filed: October 26, 2010
    Publication date: April 28, 2011
    Inventors: Michel Anciaux, Fabienne Mathieu, Frédéric Lin, Martin Poncelet
  • Publication number: 20110020942
    Abstract: Described herein are methods for determining an amount of an analyte in a test sample. The methods involve preparing a calibration curve using standard samples containing an isotopically-labeled standard in a biological matrix.
    Type: Application
    Filed: July 26, 2010
    Publication date: January 27, 2011
    Inventors: Blas Cerda, Alex Cherkasskiy
  • Patent number: 7871572
    Abstract: Provided are mixed ligand metal nanoparticle chemical sensors in which metal nanoparticles are encapsulated by at least two kinds of different molecule ligands having a relatively low conductivity and various composition ratios, and a chemical sensor array in which a film of the metal nanoparticle sensor is formed on the substrate. The metal nanoparticle sensor using the mixed ligand improves sensitivity and reaction speed with respect to an analyte, and selectivity with respect to various analytes, and a kind and a composition of a ligand of the mixed ligand constituting the metal nanoparticle sensor are adjusted to allow the high sensitivity nanoparticle sensor to be applied to the sensor array technology, thereby enabling a design of sensor properties as well as systematic access to a configuration of the sensor array the most efficient for the analytes.
    Type: Grant
    Filed: September 21, 2004
    Date of Patent: January 18, 2011
    Assignee: Electronics and Telecommunications Research Instutute
    Inventors: Young Jun Kim, Yong Shin Kim, Yoon Seok Yang, Seung Chul Ha, Hae Sik Yang, Yun Tae Kim, Dae Sik Lee, Young Sik Hong
  • Patent number: 7851624
    Abstract: Provided is a rosuvastatin triol and its use as a reference standard for analysis of rosuvastatin. Also provided are methods for preparation of rosuvastatin.
    Type: Grant
    Filed: March 13, 2008
    Date of Patent: December 14, 2010
    Assignee: Teva Pharamaceutical Industries Ltd.
    Inventors: Valerie Niddam-Hildesheim, Anna Balanov, Irena Veinberg