Abstract: The invention relates to a method for analysis of liposoluble vitamins of biological materials. It is proposed to perform reagent storage, sample application and conducting of the measuring in one reaction vessel and to correct interfering ingredients during the photometric evaluation.

Abstract: A method of monitoring breakthrough of an adsorbent bed consisting for example of ion-exchange resins, used during a stage of purification of an ester stream containing glycerin, obtained for example in a vegetable or animal oil and aliphatic monoalcohol transesterification process, permits the instantaneous detection of the presence of glycerol (glycerin) in the ester stream by arranging a turbidimeter at the adsorbent bed outlet.

Abstract: An improved qualitative or semi-quantitative diagnostic test for low levels of any analyte, such as hCG, in a biological sample, such as urine. The test comprises of a test device containing reagents for the detection of the monitored analyte and an electronic reader that measures color development at a detection area of the device. The color development is converted to an electronic or digital signal. Improvements were made to the detection process to optimize the detection of a valid fluid front, increase the detection limit without compromising the reliability and accuracy of the test system, and improve the determination of test result validity.

Abstract: The present invention relates in particular to methods of detecting predisposition to or diagnosis and/or prognosis of Charcot-Marie-Tooth (CMT) and related disorders. More specifically, the invention relates to development, validation and application of new biomarkers, which can be used for detecting the presence or risk of CMT disease and related disorders. In particular, the present invention relates to metabolite, lipid, carbohydrate and proteinaceous biomarkers that can be measured in biological body fluids and easily available extracts of biopsies, which can be used to aid in the detection, prediction of drug treatment and follow up of this treatment of neurodegenerative disorders, including CMT disease. The present invention also relates to methods for identification of CMT disease subtypes, assessing the responsiveness to the treatments and the efficacy of treatments in subjects having CMT or a related disorder.

Abstract: A method of determining the functional activity of the MRP2 and/or MRP3 efflux pathway of a human or animal subject was disclosed. The method comprises (i) determining the level of a bile acid derivative in the blood of said human or animal subject at a predetermined time interval after introducing an amount of the bile acid derivative into the subject, and (ii) using the determination obtained in step (i) to indicate the functional activity of the MRP2 and/or MRP3 efflux pathway of the subject.

Abstract: The invention provides a method of determining the amount of an analyte having an oxidation potential, for a one electron oxidation process, of about +0.10 to about +1.20 volts at pH 7, relative to the normal hydrogen electrode at 298K, said method comprising measuring the emission intensity or emission lifetime, at two or more wavelengths, from a sample comprising said analyte and two or more different macrocyclic lanthanide (III) complexes, wherein each of said macrocyclic lanthanide (III) complexes comprises a different lanthanide ion but the same macrocyclic ligand, and using a ratio of emission intensities or emission lifetimes measured at two different wavelengths to calculate the amount of analyte in said sample.

Abstract: A method of estimating the amount of unreacted starting materials (glycerides, methyl esters, etc.) and the composition of a biodiesel using TLC in conjunction with a lipophilic dye, Nile Red is described herein. The dye based TLC method of the present invention is convenient and provides significant advantages over existing methods for estimating the purity of a biodiesel composition.

Abstract: Disclosed are methods and systems for the analysis of delta 5 steroid compounds in a sample using supported liquid extraction and liquid chromatography/mass spectrometry.

Abstract: Provided are methods of detecting the presence or amount of a dihydroxyvitamin D metabolite in a sample using mass spectrometry. The methods generally comprise ionizing a dihydorxyvitamin D metabolite in a sample and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample. In certain preferred embodiments the methods include immunopurifying the dihydroxyvitamin D metabolites prior to mass spectrometry. Also provided are methods to detect the presence or amount of two or more dihydroxyvitamin D metabolites in a single assay.

Abstract: A method of monitoring water-soluble treatment chemicals in a fluid that is immiscible with water and which may or may not contain some aqueous fluid, the method using at least one reagent that produces an optically detectable product, the detection step can take place without separation of the aqueous phase containing the treatment chemicals from the fluid immiscible with water.

Abstract: Provided are methods of detecting the presence or amount of a vitamin D metabolite in a sample using mass spectrometry. The methods generally comprise ionizing a vitamin D metabolite in a sample and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample. Also provided are methods to detect the presence or amount of two or more vitamin D metabolites in a single assay.

Abstract: Described herein are methods for determining an amount of an analyte in a test sample. The methods involve preparing a calibration curve using standard samples containing an isotopically-labeled standard in a biological matrix.

Abstract: The present invention provides a device for the detection of a peroxide-based explosive, in particular, triacetone triperoxide (TATP), which is based on a molecular controlled semiconductor resistor (MOCSER) and composed of at least one insulating or semi-insulating layer, at least one conducting semiconductor layer, two conducting pads and a layer of multifunctional organic molecules capable of adsorbing molecules of the peroxide-based explosive. The invention further provides an array of semiconductor devices for the selective detection of a peroxide-based explosive, as well as a method for the selective detection of vapors of a peroxide-based explosive in a gaseous mixture using this array.

Abstract: Embodiments of the present invention are directed to methods and kits for determining the presence or absence, and the amount if present, of vitamin D analogs in samples. One embodiment of the present method for detecting the presence or absence of vitamin D analogs in a sample comprises the steps of adding an effective amount of a multiple charge cationic agent to the sample to form a cationic treated sample. The effective amount of a multiple charge cationic agent enhances the signal from vitamin D analogs upon analysis by mass spectroscopy.

Abstract: A reagent composition for releasing vitamin D compounds bound to vitamin D-binding protein, an in vitro method for the detection of a vitamin D compound in which the vitamin D compound is released from vitamin D-binding protein by the use of this reagent composition and the reagent mixture obtained in this manner. The use of the disclosed reagent composition to release vitamin D compounds as well as a kit for detecting a vitamin D compound which contains the reagent composition for releasing vitamin D compounds in addition to common detecting reagents.

Abstract: A test module for performing a genetic diagnostic assay, the test module having an outer casing dimensioned for hand-held portability, the outer casing having a receptacle for a biological sample containing target nucleic acid sequences, an array of chambers containing probes for hybridization with the target nucleic acid sequences to form probe-target hybrids, a flow-path extending from the inlet to the probes, and, a reagent reservoir containing a reagent for addition to the sample in the flow-path upstream of the probes, wherein, each of the chambers contains less than 270 picograms of probe and the reagent reservoir has a volume less than 1000,000,000 cubic microns.

Abstract: Methods for determining the amount of vitamin D compounds in a sample are provided. The methods can employ LC-MS/MS techniques and optionally the use of deuterated internal standards. Methods for diagnosing vitamin D deficiencies are also provided.

Abstract: Provided is a diagnosis method for an oil-filled electrical apparatus, which is for evaluating the degree of danger of copper sulfide being generated within the oil-filled electrical apparatus, comprising: a first step for detecting a specific compound contained within insulating oil inside the oil-filled electrical apparatus; a second step for evaluating the possibility of copper sulfide being generated inside the oil-filled electrical apparatus, on the basis of the result detected by the first step; and a third step for diagnosing the degree of danger of a malfunction occurring in the oil-filled electrical apparatus, on the basis of the evaluation result obtained in the second step. The specific compound contains dibenzyl disulfide and/or a reaction product of a radical resulting from dibenzyl disulfide, and di-tert-butyl-p-cresol and/or a reaction product of a radical resulting from di-tert-butyl-p-cresol, or, di-tert-butyl-phenol and/or a reaction product of a radical resulting from di-tert-butyl-phenol.

Abstract: A vapochromic gold-copper complex [AuL2(Cu(Y)n)2](X)3 exhibiting luminescence is provided, where L is an N-heterocyclic carbene; Y is a heteroatom-containing ligand; X is an anion, and n is an integer having a value of 1 or 2, and solvates thereof. A reaction of [AuL2(Cu(Y)n)2](X)3 with water vapor or an organic compound vapor, for example, affords a modified complex that yields a change in luminescence color under UV excitation. These tricationic vapochromic materials exhibit large changes in the emission through ligand substitution reactions between the solid complex and vapors, which permit use in luminescent vapochromic sensors.

Abstract: In one aspect a method of determining the functional activity of the MRP2 and/or MRP3 efflux pathway of a human or animal subject, comprises: (i) determining the level of a bile acid derivative in the blood of said human or animal subject at a predetermined time interval after introducing an amount of the bile acid derivative into the subject; and (ii) using the determination obtained in step (i) to indicate the functional activity of the MRP2 and/or MRP3 efflux pathway of the subject.

Abstract: Described herein are methods for determining an amount of an analyte in a test sample. The methods involve preparing a calibration curve using standard samples containing an isotopically-labeled standard in a biological matrix.

Abstract: The present invention relates to a cartridge 1 including a plurality of analyzing tools 3 arranged lined in a plane direction and a case 2 for accommodating the plurality of analyzing tools 3, and being configured to take out the analyzing tool 3 one at a time from the case 2. The plurality of analyzing tools 3 further include engagement means 32, 33 for restricting the analyzing tools 3 adjacent to each other in the plane direction and allowing removable attachment in a thickness direction D1, D2 of the analyzing tool 3. The present invention further relates to an analyzer and an analyzing system for analyzing a sample using the cartridge 1.

Abstract: Provided are methods of detecting the presence or amount of a dihydroxyvitamin D metabolite in a sample using mass spectrometry. The methods generally comprise associating an amine with a dihydroxyvitamin D metabolite in a sample, ionizing the adduct, and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample.

Abstract: Implementations and techniques for sensing hydroxyl radicals in ozone washing systems are generally disclosed.

Abstract: Compositions and methods for inhibiting the sprouting of potato tubers are provided. The compositions comprise C3 to C14 aliphatic aldehydes and ketones, and/or C3 to C7 primary and secondary aliphatic alcohols.

Abstract: Described herein are methods for determining an amount of an analyte in a test sample. The methods involve preparing a calibration curve using standard samples containing an isotopically-labeled standard in a biological matrix.

Abstract: Provided is a method for quantitative analysis of carotenoids such as ?-cryptoxanthin contained in a sample such as citrus fruit or the like which enables simple and accurate quantitative analysis. An internal standard such as lycopene or retinal is used, whereby work is facilitated and an error of a measured value is reduced. Further, a dehydrating agent is added during saponification reaction in the preparation of a sample for analysis, whereby a deviation of a measured value can be reduced.

Abstract: Provided are methods of detecting the presence or amount of a dihydroxyvitamin D metabolite in a sample using mass spectrometry. The methods generally comprise ionizing a dihydorxyvitamin D metabolite in a sample and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample. In certain preferred embodiments the methods include immunopurifying the dihydroxyvitamin D metabolites prior to mass spectrometry. Also provided are methods to detect the presence or amount of two or more dihydroxyvitamin D metabolites in a single assay.

Abstract: A method for the equilibration of enriched isotope species and natural isotope species prior to mass spectrometric analysis using solid phase and/or microwave isotope ratio equilibration and measurement.

Abstract: The present invention concerns methods and compositions to prepare biological samples to preserve the macromolecules in the samples. Embodiments of the invention concern the use of a soak solution that contains one or more water-miscible solvents. A sample is incubated with the soak solution to the point of saturation at a temperature above the melting temperature of the water-miscible solvent but below 0° C. The use of methods and compositions of the invention allow for subsequent preparation or analysis of the samples.

Abstract: The present invention provides a method for detection or determination of vitamin C by luminescence that employs a redox-responsive fluorogenic probe consisting of porphyrin or phthalocyanine with a nitroxide radical(s), or that employs a liposome preparation comprising the said redox-responsive fluorogenic probe. The present method can overcome the problems of prior art methods, and in particular, are applicable for bioimaging to allow elucidation of the behavior of vitamin C in vivo.

Abstract: Methane gas in a ruminant exhalation may be oxidized to reduce the amount of methane gas output by the ruminant.

Abstract: Embodiments of the present invention provide for detecting and/or screening for conditions associated with altered sterols by derivatization of sterols to provide suitable sensitivity and selectivity of detection using tandem mass spectrometry with electrospray ionization. Such testing may be performed to detect and/or screen for conditions such as Smith-Lemli-Opitz syndrome, familial hypercholesterolaemia, and cerebrotendinous xanthomatosis, or certain bile acid disorders.

Abstract: A diagnostic method using biomarkers to predict future adverse coronary events is provided. More particularly, the present invention is directed to diagnostic tests for characterizing an individual's risk of developing or having cardiovascular disease. In certain embodiments, the method of the present invention quantitates the presence of elevated levels of chlorinated lipids derived from myeloperoxidase as a prognostic indicator of future adverse coronary events.

Abstract: The present invention relates to a method for determining whether or not a mammal is affected with a lung cancer. The method for determining whether or not a mammal is affected with a lung cancer of the present invention includes the steps of: (a) detecting whether or not anthranilic acid is contained in the urine excreted from the mammal; and (b) determining that the mammal is affected with a lung cancer when the anthranilic acid is detected as being contained in the urine.

Abstract: Provided are methods of detecting the presence or amount of a vitamin D metabolite in a sample using mass spectrometry. The methods generally comprise ionizing a vitamin D metabolite in a sample and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample. Also provided are methods to detect the presence or amount of two or more vitamin D metabolites in a single assay.

Abstract: The invention relates to computational methods, systems and apparatus useful in the analysis of sets of biomolecules in an accessible body fluid or tissue sample from a patient, which biomolecules collectively or individually are candidates to serve as biomarkers, i.e., biomolecules which together or individually upon detection or change are indicative that the patient is in some biological state, such as a diseased state. The methods permit one to examine such potential biological markers to determine whether each one is indeed present as a consequence of the biological state, or as an artifact of the biomarker search protocol.

Abstract: Articles and methods for the detection and/or quantification of organic analytes are described. The invention provides a chemical indicator test strip, comprising: a substrate; and a coating comprising a leuco dye complex on the substrate, the coating being insoluble in water and reactive with an organic analyte, the coating derived from a solution of leuco dye and developing agent. In another aspect, the coating further comprises adjuvant, in the form of a water-insoluble, polar, hydrophobic, aprotic material that extends the lower limit of detection for the analyte. In still another aspect, the present invention provides a method of using a chemical indicator test strip, comprising: (a) exposing the chemical indicator test strip to a sample comprising an organic analyte; (b) measuring a color change on the chemical indicator test strip following the exposing step; and (c) correlating the color change with the concentration of the analyte in the sample.

Abstract: Electrodeposition baths, systems and methods are provided. In some embodiments, the baths, systems and methods are used to deposit metal alloy coatings.

Abstract: Methods for determining the amount of DHVD2 and/or DHVD3 in a sample are provided. The methods can employ LC-MS/MS techniques coupled with sample affinity purification and derivatization steps. Methods for diagnosing vitamin D deficiencies are also provided.

Abstract: The present invention relates to a method of measuring a vitamin D metabolite in a sample, the method comprising the steps of (a) treating said sample with a vitamin D metabolite releasing reagent under conditions appropriate to release a vitamin D metabolite from vitamin D-binding protein and not to cause protein precipitation, (b) subjecting the treated sample obtained in step (a) to a chromatographic separation, and (c) measuring a vitamin D metabolite during or after said chromatographic separation. The present invention also relates to methods for determining the vitamin D status of a subject, for use in the diagnosis of disease, and to agents and kits for use in performing the methods of the invention.

Abstract: The present disclosure relates to a method for assessing if a patient is affected with a lung cancer. The method includes analyzing the presence and amount of ?-citronellol contained in the urine excreted from the patient and determining if the presence and amount of ?-citronellol is indicative of lung cancer.

Abstract: Embodiments of the present invention are directed to methods and kits for determining the presence or absence, and the amount if present, of vitamin D analogs in samples. One embodiment of the present method for detecting the presence or absence of vitamin D analogs in a sample comprises the steps of adding an effective amount of a multiple charge cationic agent to the sample to form a cationic treated sample. The effective amount of a multiple charge cationic agent enhances the signal from vitamin D analogs upon analysis by mass spectroscopy.

Abstract: A detection method of blood plasma schizadrin B of dissipating blood stasis botanical is disclosed. The method includes: (1) extracting schizadrin B from plasma of mammalian administered dissipating blood stasis botanical by ethyl acetate with the volume ratio 1:4, whirling 3-5 mins, centrifugating at 9600 rpm for 10 mins, drying and enriching the upper layer at 25-30° C., and redissolving with mobile phase; (2) UPLC/MS measuring: UPLC condition: chromatographic column: Acquity UPLC BEH C18, 2.1?100 mm, mobile phase A: water-acetonitrile-formic acid 95:5:0.1 v/v/v, mobile phase B: acetonitrile-formic acid 100:0.1 v/v; MS condition: electric spraying ion source (ESI), detecting with positive ion mode, scanning at the range of m/z 150-1200. The method can be used for pharmacokinetics study of schizadrin B in dissipating blood stasis botanical.

Abstract: Quantification of vitamin D2, vitamin D3, and the monohydroxy and diihydroxy metabolites of vitamin D2 and vitamin D3, can comprise labeling analytes with mass spectrometry (MS) tagging reagents and performing LC-MSMS analysis of the labeled analytes. The labeled analytes can include a labeled standard and can have distinct retention times on a reversed phase column, as well as distinct masses. Under high energy collisions, reporter groups can be generated. The intensity or the peak area detected for each reporter group can be used for quantitation. In some embodiments, a one-step tagging reagent is used that is a dienophile-containing, labeled Diels Alder reagent.

Abstract: The present invention provides a low-cost, easy-to-use, and accurate method and apparatus for determining alcohol content in a test sample, such as wine. The method for alcohol content determination includes the following steps. A known quantity of a test sample is provided. A measurement of electrolytic conductivity EC0 of the test sample is taken. Next, a known quantity of conductive additive with known composition is added to the test sample to produce a first solution. A measurement of Electrolytic conductivity EC1 is taken for the first solution. Data representing predetermined values for EC0 and EC1 with corresponding known alcohol concentrations is provided for comparison. The electrolytic conductivity values for EC0 and EC1 are cross-referenced against the known data to determine a value for the alcohol concentration of the test sample. In addition, the values for EC0 and EC1 are adjusted for temperature and other variables.

Abstract: Provided are methods of detecting the presence or amount of a vitamin D metabolite in a sample using mass spectrometry. The methods generally comprise ionizing a vitamin D metabolite in a sample and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample. Also provided are methods to detect the presence or amount of two or more vitamin D metabolites in a single assay.

Abstract: Provided are methods of detecting the presence or amount of a dihydroxyvitamin in D metabolite in a sample using mass spectrometry. The methods generally comprise ionizing a dihydroxyvitamin D metabolite in a sample and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample. In certain preferred embodiments the methods include immunopurifying the dihydroxyvitamin D metabolites prior to mass spectrometry. Also provided are methods to detect the presence or amount of two or more dihydroxyvitamin D metabolites in a single assay.

Abstract: Electrodeposition baths, systems and methods are provided. In some embodiments, the baths, systems and methods are used to deposit metal alloy coatings.

Abstract: The invention relates to compositions, kits and methods used in hydroxyl radical detection. In some embodiments, the invention relates to compositions comprising a dye preferably methylene blue immobilized on a substrate. In additional embodiments, the invention relates methods of correlating color changes of a dye to the presence or absence of hydroxyl radicals. In some embodiments, the invention relates to a methylene blue dye containing test strip and its use in a method for detecting the presence of hydroxyl radicals.