Metal Or Metal Coated Patents (Class 436/525)
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Patent number: 8568993Abstract: A diagnostic method for determining the absence or presence of a disease is provided. The method includes assaying the amount and/or types of glycopeptides in a sample from a subject, and comparing these to the amount and types of reference glycopeptides. The method may include the use of a stable isotope label, affinity selection, immunoaffinity chromatography, and glycoproteomics techniques, to identify and quantify changes in glycosylated peptides or glycosylated proteins associated with cancers such as malignant lymphoma or breast cancer, to monitor patient's response to therapy, and to monitor disease recurrence.Type: GrantFiled: June 30, 2008Date of Patent: October 29, 2013Assignee: Purdue Research FoundationInventors: Fred E. Regnier, Stephen B. Hooser, Christina R. Wilson, Wonryeon Cho
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Patent number: 8569078Abstract: The present invention provides novel compositions of binding moiety-nanoparticle conjugates, aggregates of these conjugates, and novel methods of using these conjugates, and aggregates. The nanoparticles in these conjugates can be magnetic metal oxides, either monodisperse or polydisperse. Binding moieties can be, e.g., oligonucleotides, polypeptides, or polysaccharides. Oligonucleotide sequences are linked to either non-polymer surface functionalized metal oxides or with functionalized polymers associated with the metal oxides. The novel compositions can be used in assays for detecting target molecules, such as nucleic acids and proteins, in vitro or as magnetic resonance (MR) contrast agents to detect target molecules in living organisms.Type: GrantFiled: May 3, 2010Date of Patent: October 29, 2013Assignee: The General Hospital CorporationInventors: Lee Josephson, Ralph Weissleder, J. Manuel Perez
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Patent number: 8563328Abstract: A method for biosensing that includes passing, via convective flow, a sample believed to contain one or more target biomarkers through a microfluidic channel and over the surface of an optical waveguide that has been prepared to bind the one or more target biomarkers, and sensing for an emission output from the optical waveguide at a wavelength that is characteristic of the binding of the target biomarker. A biosensor device that includes a module defining at least one microfluidic channel, an optical waveguide exposed along at least a portion of its length to fluid flow within the microfluidic channel, where a surface of the optical waveguide being prepared to bind a target biomarker, and an excitation source to couple an excitation wavelength of light into the optical waveguide. The device also includes a sensor for detecting emission light from the optical waveguide at an emission wavelength characteristic of binding of the target biomarker.Type: GrantFiled: February 16, 2006Date of Patent: October 22, 2013Assignee: University of Louisville Research Foundation, Inc.Inventors: Kyung Aih Kang, Chong H. Ahn
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Patent number: 8563329Abstract: Modified branched polymers are combined with bioactive agents which are one member of a binding pair for use in an assay.Type: GrantFiled: May 2, 2006Date of Patent: October 22, 2013Assignee: ANP Technologies, Inc.Inventors: Ray Yin, Dujie Qin, Jing Pan
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Patent number: 8557604Abstract: A lateral flow, membrane-based assay device for detecting the presence or quantity of an analyte residing in a test sample is provided. The device utilizes phosphorescence to detect the signals generated by excited phosphorescent labels. The labels may have a long emission lifetime so that background interference from many sources, such as scattered light and autofluorescence, is practically eliminated during detection. In addition, the phosphorescent labels may be encapsulated within particles to shield the labels from quenchers, such as oxygen or water, which might disrupt the phosphorescent signal.Type: GrantFiled: December 14, 2007Date of Patent: October 15, 2013Assignee: Kimberly-Clark Worldwide, Inc.Inventor: Xuedong Song
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Patent number: 8557608Abstract: A method is disclosed for characterizing a local magnetic field, in particular a stray field caused by a magnetizable or magnetic particle in a prescribed measuring area of a magnetic field. In at least one embodiment, a sensor array including a number of magnetic sensors and each having at least one layer sensitive to magnetic fields is arranged at least in a subregion of the measuring area, and at least one device is provided for reading out separately the signal from each sensor. According to at least one embodiment, the size, in particular of XMR sensors and the surface of the layer sensitive to magnetic fields and the grid dimension of the sensor array, are selected such that at least two neighboring sensors are influenced by the local stray field. An associated device for carrying out the method is also disclosed.Type: GrantFiled: November 22, 2006Date of Patent: October 15, 2013Assignee: Siemens AktiengesellschaftInventor: Joachim Bangert
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Patent number: 8551716Abstract: The invention provides methods of detecting a change in cell growth patterns, methods of screening many different antibodies in one receptacle, and methods of detecting specific binding of an antibody to a protein or cell, wherein the antibody is in a mixture of many different antibodies.Type: GrantFiled: June 19, 2012Date of Patent: October 8, 2013Assignee: X-Body, Inc.Inventors: Christine C. Genick, Lance G. Laing, Peter Li, Timothy F. Smith, Lara Madison, Bo Lin
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Patent number: 8546092Abstract: An embodiment relates to a method of detecting a drug resistance in a patient comprising adding nanoparticles to sample platelets to form activated platelets containing the nanoparticles and comparing a difference in activation of the activated platelets and the sample platelets. Another embodiment relates to a method of monitoring a thrombotic risk factor in a subject in a general population comprising adding nanoparticles to sample platelets to form activated platelets containing the nanoparticles and comparing a difference in activation of the activated platelets and the sample platelets. Yet another embodiment relates to a kit comprising nanoparticles, a fluorescence dye tagged antibody and optionally a buffer, wherein the kit is configured to detect a drug resistance in a patient or a likelihood of the thrombotic risk factor in a subject in general population.Type: GrantFiled: December 7, 2009Date of Patent: October 1, 2013Assignee: University of CalcuttaInventors: Suryyani Deb, Anjan Dasgupta, Prabir Lahiri
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Patent number: 8535949Abstract: This invention relates to magnetic resonance-based sensors and related methods.Type: GrantFiled: November 4, 2009Date of Patent: September 17, 2013Assignee: The General Hospital CorporationInventors: Lee Josephson, Yi Sun, Ralph Weissleder
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Patent number: 8530230Abstract: The present invention is directed to methods for conducting multiplexed assays. The methods are particularly well suited for measuring a plurality of analytes that may be present in very different abundances. The invention also relates to systems, devices, equipment, kits and reagents for use in such methods.Type: GrantFiled: July 16, 2012Date of Patent: September 10, 2013Assignee: Meso Scale Technologies, LLCInventors: Selen A. Stromgren, Eli N. Glezer
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Patent number: 8530170Abstract: The present invention is directed to electronic methods of detecting target analytes such that upon binding of the target analyte a shift in electrochemical potential is seen. This assay relies on the use of an electroactive moiety (“EAM”) that is attached to an electrode and comprises a self-immolative moiety, whose presence gives the EAM a first E0, and whose absence, upon irreversible cleavage gives the EAM a second E0. This difference is detected, and if such change occurs, it is an indication of the presence of a target analyte.Type: GrantFiled: August 9, 2010Date of Patent: September 10, 2013Assignee: OHMX CorporationInventor: Paul A. Bertin
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Patent number: 8526001Abstract: An instrument for measuring and analyzing surface plasmon resonance on a sensor surface has a polarized light source optically connected to the sensor surface by a plurality of optical elements, including in one embodiment an optical telescope that transfers light from a rotatable reflecting surface to the sensor surface. Selective positioning of a cylindrical lens into a first position within the path of light transforms collimated light to a rectangular wedge that is incident upon the sensor surface at numerous angles. In another embodiment, the light source is operated as a laser to excite fluorescence on the sensor surface and the fluorescence is selectively directed to a detector by appropriate optical elements positioned in specific configurations.Type: GrantFiled: January 28, 2013Date of Patent: September 3, 2013Assignee: Ciencia, Inc.Inventors: George N. Gibson, Ernest F. Guignon, Michael T. Reilly
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Publication number: 20130224886Abstract: A flow rate measurement apparatus includes a light oscillator; a thin metallic film which causes surface plasmon resonance by light output from the light oscillator; a focusing unit which fixes the thin metallic film and converts the output light of the light oscillator into incident light having a plurality of incident angles to focus the incident light at a location of a focal line in a straight line shape on the thin metallic film; a measurement part having antibody fixed areas to which an antibody is fixed and reference areas to which an antibody is not fixed, the antibody fixed areas and the reference areas being alternately arranged at a location along the focal line location on the thin metallic film; a light receiver which receives reflected light, at the focal line location, of the output light by surface plasmon resonance occurring at the focal line location, at each of the plurality of incident light angles; an SPR angle calculator which obtains a temporal change of an SPR angle in each of the antiType: ApplicationFiled: April 5, 2013Publication date: August 29, 2013Applicant: Nippon Telegraph and Telephone CorporationInventor: Nippon Telegraph and Telephone Corporation
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Patent number: 8518709Abstract: In a method for regenerating a biosensor, a biosensor is prepared having a substrate surface on which at least one receptor is immobilized. At least one ligand that is binding specific to the receptor is bound to the receptor, said ligand, together with the receptor, forming a ligand-receptor complex. To regenerate the biosensor, the ligand-receptor complex is brought into contact with an enzyme. The enzyme is selected so that it catalyzes the ligand into fragments. The enzyme is inert with respect to the receptor.Type: GrantFiled: November 17, 2009Date of Patent: August 27, 2013Assignee: Endress+Hauser Conducta Gesellschaft fuer Mess-und Regeltechnik mbH+Co. KGInventors: Sonja Bednar, Johannes Baader, Holger Klapproth, Ingo Freund
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Patent number: 8513030Abstract: A universal chirality sensor based on immuno-recognition-driven nanoparticle assembly has been fabricated. The design of smart 10 nm AuNP-antigen and 20 nmAuNP-antibody described for the detection of aflatoxin B1. 10 nm AuNP-antigen and 20 nmAuNP-antibody assemble to symmetric plasmonic nanoparticle dimers, which induced CD signal. The addition of aflatoxin B1 to the chirality sensor resulted in transverse CD signal compared to a blank control as shown by CD measurements. This process also allowed the rapid and facile determination of concentrations of aflatoxin B1 in drinking water (tap water). Good linearity for all calibration curves was obtained, and the limit of detection (LOD) for aflatoxin B1 was 0.02 ng/mL in tap water.Type: GrantFiled: May 4, 2012Date of Patent: August 20, 2013Inventors: Libing Wang, Chuanlai Xu, Zhou Xu
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Fluorescent substance-containing silica nanoparticles with coating having high bulk refractive index
Patent number: 8513031Abstract: Disclosed are fluorescent substance-containing silica nanoparticles containing a fluorescent substance therein featured in that the surface of the silica nanoparticles is coated with a material having a bulk refractive index of from 1.60 to 4.00. The invention can provide fluorescent substance-containing silica nanoparticles excellent in long term storage stability and a biosubstance labeling agent employing the same.Type: GrantFiled: November 4, 2010Date of Patent: August 20, 2013Assignee: Konica Minolta Medical & Graphic, Inc.Inventors: Takuji Aimiya, Hideki Hoshino -
Patent number: 8513002Abstract: Spectroscopic analysis systems and methods for analyzing samples are disclosed. An analysis system may contain an electromagnetic radiation source to provide radiation, a spectroscopic analysis chamber to perform a coherent Raman spectroscopy (e.g., stimulated Raman or coherent anti-Stokes Raman spectroscopy), and a radiation detector to detect radiation based on the spectroscopy. The chamber may have a resonant cavity to contain a sample for analysis, at least one window to the cavity to transmit the first radiation into the cavity and to transmit a second radiation out, a plurality of reflectors affixed to a housing of the cavity to reflect radiation of a predetermined frequency, the plurality of reflectors separated by a distance that is sufficient to resonate the radiation. The spectroscopic analysis system may be coupled with a nucleic acid sequencing system to receive a single nucleic acid derivative in solution and identify the derivative to sequence the nucleic acid.Type: GrantFiled: July 19, 2011Date of Patent: August 20, 2013Assignee: Intel CorporationInventors: Andrew Arthur Berlin, Christopher Marc Gerth, Tae-Woong Koo
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Patent number: 8507212Abstract: A process for treating biological targets in a fluid of a biological organism, including introducing a fluid comprising a biological target to an assembly comprising an inlet connected to receive the fluid and an outlet connected to pass the fluid from the assembly, wherein the assembly comprises a flow chamber for conveying a flow of the fluid, and a capture zone comprising a target-specific binding agent, wherein during flow of the fluid through the flow chamber, the biological target undergoes flux rolling along the target-specific binding agent.Type: GrantFiled: January 10, 2012Date of Patent: August 13, 2013Assignee: Biomed Solutions LLCInventors: Patrick R. Connelly, Jeffrey L. Helfer, Andrew W. Custer, Michael B. Kim
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Patent number: 8497131Abstract: Surface Enhanced Spectroscopy-Active Composite Nanoparticles (SACN) comprising: core/shell nanoparticles comprising nanoparticle cores covered with shells, wherein the cores and the shells may comprise the same or different materials; at least one Raman-active reporter molecule associated with said core/shell nanoparticle; and an SiO2 encapsulant which encapsulates the core/shell nanoparticle and the at least one Raman-active reporter molecule, are described.Type: GrantFiled: April 25, 2005Date of Patent: July 30, 2013Assignee: Becton, Dickinson and CompanyInventors: Michael J. Natan, Sharron Gaynor Penn, R. Griffith Freeman, Gabriela Chakarova, William E. Doering, Ian D. Walton
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Patent number: 8492166Abstract: A particle suspension for use in immunoassay, comprising: particles for use in immunoassay; and a silicone antifoam agent, is disclosed. And a reagent kit for use in immunoassay, comprising: a reagent containing particles and a silicone antifoam agent; another reagent containing an antigen or antibody capable of binding to a target substance and particles; and a further reagent containing a labeled antigen or antibody capable of binding to the target substance, is disclosed.Type: GrantFiled: August 9, 2010Date of Patent: July 23, 2013Assignee: Sysmex CorporationInventor: Toshiyuki Baba
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Patent number: 8492167Abstract: Methods and apparatuses for determining whether a fluid has been introduced into an assay measurement apparatus involving delivering a fluid to a surface of a resonant device. The methods also involve monitoring an electrical signal output by the resonant device, wherein properties of the electrical signal vary based on physical properties of the fluid in contact with the surface of the resonant device and determining if the electrical signal output by the resonant device satisfies a predetermined condition indicative of the presence of the fluid.Type: GrantFiled: November 21, 2006Date of Patent: July 23, 2013Assignee: BioScale, Inc.Inventors: Brett P. Masters, Michael F. Miller
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Patent number: 8492139Abstract: This invention relates to a detection system for measuring a fluorescent signal in a fluorescent assay. The system comprises a probe having a small sensing surface bound with a fluorescent label, and a light source and a detector both mounted at the proximal side of the sensing surface of the substrate. The invention also relates to a method for detecting an analyte in a liquid sample using a probe tip having a small surface area (?5 mm) and a high molecular weight polymer (?1 MD) having multiple binding molecules and multiple fluorescent labels. The binding reaction is accelerated by flowing the reaction solutions laterally and moving the probe tip up and down in the reaction vessels. The invention furthers relates to a fluorescent labeling composition comprising a cross-linked FICOLL® molecule having a plurality of binding molecules and a plurality of fluorescent labels.Type: GrantFiled: October 24, 2012Date of Patent: July 23, 2013Assignee: Access Medical Systems, Ltd.Inventors: Hong Tan, Robert F. Zuk, Yushan Tan, Erhua Cao, Min Xia, Jun Chen
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Patent number: 8486720Abstract: The present invention provides a method for the generation of novel libraries of encoded magnetic particles from sub-libraries of by the generation of novel sub-libraries of magnetic nanoparticles and encoded particles. The sub-libraries are functionalized on demand are useful in the formation of arrays. The present invention is especially useful for performing multiplexed (parallel) assays for qualitative and/or quantitative analysis of binding interactions of a number of analyte molecules in a sample.Type: GrantFiled: August 3, 2004Date of Patent: July 16, 2013Assignee: BioArray Solutions, Ltd.Inventors: Sukanta Banerjee, Michael Seul, Alice X. Li
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Patent number: 8481335Abstract: The invention is directed to a sensor system including at least one sensor and target specific receptors bound substrates for purposes of enhancing detection sensitivity. Optionally, the sensor system may include quantum dots for independently verifying the presence of a target molecule or compound. The sensor system may be particularly beneficial in the field of medical diagnostics, bio-defense, food safety, water safety and general chemical detection.Type: GrantFiled: October 3, 2008Date of Patent: July 9, 2013Assignee: Drexel UniversityInventors: Wei-Heng Shih, Wan Y. Shih
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Patent number: 8481334Abstract: The invention features a method of attaching a ligand that has a free carboxyl group to a solid support by adding an amino group to the ligand to form a ligand-amino derivative, converting the ligand amino derivative to a ligand sulfhydryl derivative, attaching the ligand sulfhydryl derivative to a protein to form a ligand-linker-protein conjugate, and applying the ligand-linker-protein conjugate to the solid support. The method is particularly useful for immobilizing small molecule ligands having a free carboxyl group, such as cloxicillin, to a lateral-flow test strip, in order to make a detection zone on the test strip that exhibits a clear signal and enhanced sensitivity.Type: GrantFiled: March 31, 2008Date of Patent: July 9, 2013Assignee: Charm Sciences, Inc.Inventor: Steven J. Saul
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Patent number: 8476007Abstract: A biosensor having an optical fiber having at least one curved portion configured to enhance penetration of evanescent waves; and one or more nanoparticles associated with the optical fiber, and configured to enhance localized surface plasmon resonance.Type: GrantFiled: February 19, 2010Date of Patent: July 2, 2013Assignee: Indian Institute of Technology BombayInventors: Vemulakonda Venkata Raghavendra Sai, Soumyo Mukherji, Tapanendu Kundu
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Patent number: 8476079Abstract: Methods and devices for reducing interference from leukocytes in an analyte immunoassay are provided. In one embodiment, a method is provided comprising the steps of amending a biological sample such as a whole blood sample with one or more leukocidal reagents that reduce or eliminate the metabolic activity of leukocytes, and performing an immunoassay on the amended sample to determine the concentration of analyte in the sample. Preferably, the sample is amended with one or more enzymes and optionally one or more enzyme substrates and cofactors.Type: GrantFiled: April 30, 2010Date of Patent: July 2, 2013Assignee: Abbott Point of Care Inc.Inventors: John Lewis Emerson Campbell, Graham Davis, John Emegbero Omakor, Adam Roger Moss, Ganesh Guhados
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Patent number: 8476082Abstract: Devices and methods for the detection of target molecules are disclosed. Devices and methods for detecting food-borne target molecules are also disclosed.Type: GrantFiled: April 12, 2012Date of Patent: July 2, 2013Assignee: Invisible Sentinel, Inc.Inventors: Nicholas A. Siciliano, Martin Joseph Bouliane
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Patent number: 8455264Abstract: A method for the fluorescent detection of a substance, the method comprising providing particles comprising a metal or a metal oxide core, wherein one or more optionally fluorescently tagged antibodies or human specific peptide nucleic acid (PNA) oligomers for binding to a substance is/are bound, directly or indirectly, to the surface of the metal or metal oxide; contacting a substrate, which may or may not have the substance on its surface, with the particles for a time sufficient to allow the antibody/PNA oligomer to bind with the substance; removing those particles which have not bound to the substrate; if the antibodies or PNA oligomers are not fluorescently tagged, contacting the substrate with one or more fluorophores that selectively bind with the antibody and/or substance, then optionally washing the substrate to remove unbound fluorophores; and illuminating the substrate with appropriate radiation to show the fluorophores on the substrate.Type: GrantFiled: March 23, 2007Date of Patent: June 4, 2013Assignee: Intelligent Fingerprinting LimitedInventors: David Russell, Richard Leggett, Emma Lee-Smith, Sue Jickells, Barbara Daniel
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Patent number: 8455263Abstract: The present invention relates to an assay device and a method for using such for the quantitative determination of an analyte, based on a test strip, which contains a porous test membrane allowing for capillary flow of the analyte and complexes of the analyte, a porous upstream membrane in fluid connection with the test membrane and a porous downstream membrane in fluid connection with the test membrane, wherein the test membrane contains a test site having immobilized thereon a ligand capable of reacting with the analyte and binding such to the test site, and two standard band sites having immobilized thereon known high and low concentrations of a calibrator agent capable of reacting with a label conjugate and binding such to the standard sites, wherein the upstream membrane has a site for the application of a sample to be analyzed, and has a site downstream from the sample application site for depositing label conjugates capable of reacting with the analyte and label conjugates capable of reacting with theType: GrantFiled: December 30, 2008Date of Patent: June 4, 2013Inventor: Jin Po Lee
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Patent number: 8455265Abstract: A surface grafted conjugated polyelectrolyte (CPE) is formed by coupling a CPE by a coupling moiety to the surface of a substrate. The substrate can be of any shape and size, and for many uses of the surface grafted CPE, it is advantageous that the substrate is a nanoparticle or microparticle. Surface grafted CPEs are presented that use silica particles as the substrate, where a modified silane coupling agent connects the surface to the CPE by a series of covalent bonds. Two methods of preparing the surface grafted CPEs are presented. One method involves the inclusion of the surface being modified by the coupling agent and condensed with monomers that form the CPE in a grafted state to the substrate. A second method involves the formation of a CPE with terminal groups that are complimentary to functionality that has been placed on the surface of the substrate by reaction with a coupling agent. The surface grafted CPEs are also described for use as biosensors and biocides.Type: GrantFiled: February 29, 2008Date of Patent: June 4, 2013Assignee: STC.UNMInventors: David G. Whitten, Sireesha Chemburu, Thomas Corbitt, Linnea Ista, Gabriel Lopez, Kirk S. Schanze, Motokatsu Ogawa, Eunkyung Ji
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Patent number: 8445212Abstract: Disclosed are a micro-fluidic structure for detecting biomolecules and a micro-fluidic device having the same. More particularly, a target material including at least two cis-diols is detected by a first material containing a boronate moiety and a second material containing another boronate moiety while generating electrical signals.Type: GrantFiled: September 2, 2010Date of Patent: May 21, 2013Assignees: Samsung Electronics Co., Ltd., Industry-Academic Cooperation Foundation, Yonsei UniversityInventors: Jong Myeon Park, Won Yong Lee, Han Nim Choi, Jung Hoon Lee
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Patent number: 8440469Abstract: A method for increasing a spectroscopic signal in a biological assay is provided. The method includes forming a suspension of magnetically attractable particles. The method also includes introducing a first magnetic field at a first location to draw the magnetically attractable particles towards the first location and form a first agglomeration. The method also includes removing the first magnetic field. The method further includes introducing a second magnetic field at a second location to draw the first agglomeration towards the second location and form a second agglomeration. The method further includes focusing an excitation source on the second agglomeration formed at the second location.Type: GrantFiled: June 18, 2007Date of Patent: May 14, 2013Assignee: Morpho Detection, Inc.Inventors: Tracy Lynn Paxon, Frank John Mondello, Yuan-Hsiang Lee, Michael Craig Burrell
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Patent number: 8440468Abstract: Provided is a method for amplifying a frequency variation of a detected signal in a biosensor that is used for detecting a biomolecule by measuring a change in frequency of an oscillating signal, the change being caused by pressure a biomolecule applies to a piezoelectric substance. The method for amplifying a frequency variation of a detected signal comprises the steps of: (a) applying a sample to a probe being fixed to an upper portion of a substrate of the biosensor to allow a biomolecule in the sample to be bound to the probe; (b) applying protein tagged with a metal particle to the biosensor to allow the protein and the biomolecule to be bound with each other; and (c) applying a metal enhancer to the biosensor to allow the metal enhancer to be bound to the metal particle having been bound to the protein.Type: GrantFiled: April 9, 2008Date of Patent: May 14, 2013Assignee: Samsung Electronics Co., Ltd.Inventors: Hun Joo Lee, Soo Suk Lee
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Patent number: 8435801Abstract: The present invention comprises compositions and methods for making monoclonal antibodies. The present invention further comprises vectors that replicate the immune system components, particularly an antigen-presenting cell (APC) element of the immune synapse. Additionally, the present invention may further comprise synthetic T-cells.Type: GrantFiled: April 20, 2011Date of Patent: May 7, 2013Assignee: Cytimmune Sciences, Inc.Inventors: Lawrence Tamarkin, Giulio F. Paciotti
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Patent number: 8426152Abstract: The present invention provides a method of detecting changes in the refractive index at the surface of a localized surface plasmon resonance (LSPR) detection system. The method includes generating an insoluble product from an enzymatic substrate using an immobilized enzyme, wherein the insoluble product accumulates at a LSPR supporting surface. The method also includes detecting changes in the reflected or transmitted light of the surface arising from the presence of the insoluble product using LSPR.Type: GrantFiled: December 28, 2007Date of Patent: April 23, 2013Assignee: LamdaGen CorporationInventors: Daniele Gerion, Randy Storer, Hiroyuki Takei
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Patent number: 8415170Abstract: The invention provides a method for continuously detecting glucose concentration in a sample, including: (a) providing a biosensor comprising a transducer and a polysaccharide covered on the surface of the transducer; (b) providing a carbohydrate binding protein solution, wherein the carbohydrate binding protein has at least one receptor, and the receptor is capable of binding to the polysaccharide and glucose; (c) mixing a sample and the carbohydrate binding protein solution to form a mixture; (d) contacting the mixture with the biosensor; (e) detecting the amount of carbohydrate binding proteins bound to the polysaccharide by the biosensor, wherein glucose concentration of the sample is inversely proportional to the amount of carbohydrate binding proteins bound to the polysaccharide; and (f) refreshing the surface of the biosensor with a high concentration glucose solution.Type: GrantFiled: March 4, 2010Date of Patent: April 9, 2013Assignee: Industrial Technology Research InstituteInventors: Kun-Feng Lee, Chien-An Chen, Hong-Wen Chang, Yuh-Jiuan Lin
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Patent number: 8404450Abstract: The present invention relates to an optical imaging system communicatively connected to a microwave energy producing source wherein the combination provides for increases in chemical reaction times and the ability to monitor the reactions in real time with sufficient resolution to view the location of intracellular components labeled with luminescent molecules as well as interaction with other biomolecules and responses to localized environmental variables in living cells and tissues during the application of a microwave field.Type: GrantFiled: June 4, 2008Date of Patent: March 26, 2013Assignee: University of Maryland, Baltimore CountyInventors: Chris D. Geddes, Michael J. R. Previte
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Patent number: 8399262Abstract: Disclosed herein are biosensors for the detection of airborne biomolecules. The biosensors include a housing, a sensing component, and optionally a sample capture component. The biosensors may utilize a gel-based detection platform.Type: GrantFiled: August 2, 2011Date of Patent: March 19, 2013Inventors: Darrel A. Mazzari, Martin A. Seitz
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Patent number: 8394600Abstract: Compositions, methods and devices for the detection of anti-lipoidal antibodies and the diagnosis of disease, for example, syphilis, are described. In particular, a method for immobilizing a lipoidal antigen, comprising cardiolipin, lecithin, and cholesterol, on a solid support (such as a nitrocellulose membrane) is described. The ability to immobilize a lipoidal antigen on a membrane satisfies a long-felt need for a membrane-based assay for the detection of anti-lipoidal antibodies. Also described are immunoassay devices for concurrently performing treponemal and non-treponemal tests for syphilis.Type: GrantFiled: March 23, 2012Date of Patent: March 12, 2013Assignee: The United States of America as represented by the Secretary of the Department of Health and Human Services, Centers for Disease Control and PreventionInventor: Arnold R. Castro
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Patent number: 8394626Abstract: The present invention includes but is not limited to a specimen collection device that includes a chamber capable of collecting a specimen, a specimen passage slot, a reservoir, a reservoir seal, and a test device. A sample or specimen added to the chamber flows through the specimen passage slot into the reservoir. Flow into the reservoir may be limited by the reservoir seal. The test device positioned within the reservoir detects the presence or concentration of an analyte within the sample or specimen.Type: GrantFiled: June 2, 2009Date of Patent: March 12, 2013Assignee: Alere Switzerland GmbHInventors: James T. Ramsey, Larry Hartselle
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Patent number: 8389297Abstract: The present invention relates to a droplet-based affinity assay device and system. According to one embodiment, a droplet microactuator is provided and includes an antibody immobilized on a surface. According to another embodiment, a droplet microactuator is provided and includes a droplet on the droplet microactuator, the droplet comprising an antibody.Type: GrantFiled: December 15, 2006Date of Patent: March 5, 2013Assignees: Duke University, Advanced Liquid Logic Inc.Inventors: Vamsee K. Pamula, Vijay Srinivasan, Michael G. Pollack, Richard B. Fair
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Patent number: 8383416Abstract: An optical sensor and method for use with a visible-light laser excitation beam and a Raman spectroscopy detector, for detecting the presence chemical groups in an analyte applied to the sensor are disclosed. The sensor includes a substrate, a plasmon resonance mirror formed on a sensor surface of the substrate, a plasmon resonance particle layer disposed over the mirror, and an optically transparent dielectric layer about 2-40 nm thick separating the mirror and particle layer. The particle layer is composed of a periodic array of plasmon resonance particles having (i) a coating effective to binding analyte molecules, (ii) substantially uniform particle sizes and shapes in a selected size range between 50-200 nm (ii) a regular periodic particle-to-particle spacing less than the wavelength of the laser excitation beam. The device is capable of detecting analyte with an amplification factor of up to 1012-1014, allowing detection of single analyte molecules.Type: GrantFiled: January 10, 2012Date of Patent: February 26, 2013Assignee: Vladimir PoponinInventor: Vladimir Poponin
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Patent number: 8383423Abstract: A peptide-coated nanoparticle that includes a nanocrystal surrounded by a graded shell that is composed of at least two different semiconductor molecules. At least one peptide is attached to the surface of the graded shell to render the nanoparticle biocompatible. The nanocrystal core and graded shell are optionally annealed with ultra violet radiation prior to and/or after attachment of the peptide(s).Type: GrantFiled: March 29, 2011Date of Patent: February 26, 2013Assignee: The Regents of the University of CaliforniaInventors: Shimon Weiss, James M. Tsay, Fabien Pinaud, Soren Doose
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Patent number: 8383422Abstract: The present invention provides an immunochromatographic device, which contains the following (a) and (b): (a) a first device part holding a first insoluble carrier used for developing a complex formed with an analyte and a labeling substance comprising a metal labeled with a first binding substance that can bind to the analyte and capturing the analyte and the labeling substance at a reaction portion containing a second binding substance that can bind to the analyte, and (b) a second device part holding a second insoluble carrier used for developing a liquid and a third insoluble carrier used for absorbing a liquid, in such a way that the first insoluble carrier does not come into contact with the second insoluble carrier and the third insoluble carrier.Type: GrantFiled: May 26, 2009Date of Patent: February 26, 2013Assignee: FUJIFILM CorporationInventors: Junichi Katada, Hideyuki Karaki, Takayoshi Oyamada
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Patent number: 8372592Abstract: The invention provides methods of detecting a change in cell growth patterns.Type: GrantFiled: September 22, 2011Date of Patent: February 12, 2013Assignee: X-Body, Inc.Inventors: Lance G. Laing, Rafael Fernandez, Rick Wagner
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Patent number: 8368897Abstract: An instrument for measuring and analyzing surface plasmon resonance on a sensor surface has a polarized light source optically connected to the sensor surface by a plurality of optical elements, including in one embodiment an optical telescope that transfers light from a rotatable reflecting surface to the sensor surface. Selective positioning of a cylindrical lens into a first position within the path of light transforms collimated light to a rectangular wedge that is incident upon the sensor surface at numerous angles. In another embodiment, the light source is operated as a laser to excite fluorescence on the sensor surface and the fluorescence is selectively directed to a detector by appropriate optical elements positioned in specific configurations.Type: GrantFiled: August 21, 2009Date of Patent: February 5, 2013Assignee: Ciencia, Inc.Inventors: Michael T. Reilly, Ernest F. Guignon, George N. Gibson
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Hybrid nanoparticles with LnOcore and carrying biological ligands, and method of preparation thereof
Patent number: 8357545Abstract: The invention concerns hybrid nanoparticles containing: a nanosphere, of mean diameter included in the range from 2 to 9 nm, of which at least 90% by weight consists of Ln2O3 where Ln represents a rare earth, optionally doped with a rare earth or an actinide, or a mixture of rare earths, or a rare earth and actinide mixture, in which at least 50% of the metal ions are rare earth ions, a coating around the nanosphere chiefly consisting of functionalized polysiloxane, having a mean thickness included in the range from 0.5 to 10 nm, preferably greater than 2 nm and no more than 10 nm, and at least one biological ligand grafted by covalent bonding to the polysiloxane coating and their method of preparation.Type: GrantFiled: March 2, 2005Date of Patent: January 22, 2013Assignees: Universite Claud Bernard Lyon I, Centre National de la Recherche Scientifique (C.N.R.S.), Institut National des Sciences Appliquees de LyonInventors: Pascal Perriat, Cédric Louis, Christophe Marquette, Rana Bazzi, Stéphane Roux, Olivier Tillement, Gilles Ledoux -
Patent number: 8354234Abstract: The present invention provides methods, compositions, and systems for mass spectrometric analysis of magnetic nanoparticles displaying ligands on their surface. For example, the present invention provides methods of screening a sample for the presence of at least one analyte using ligand conjugated magnetic nanoparticles, magnetic separation, and mass spectrometric analysis. The present invention also relates to MALDI matrix compositions comprising ligand conjugated magnetic nanoparticles.Type: GrantFiled: July 17, 2006Date of Patent: January 15, 2013Assignee: Academia SinicaInventors: Yu-Ju Chen, Po-Chiao Lin, Chun-Cheng Lin, Shu-Hua Chen, Po-Hung Chou, Hsin-Kai Liao
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Patent number: 8323580Abstract: A micro structure for sensing a substance using light scattering includes a substrate, a first layer on the substrate, wherein the first layer comprises a metallic material, a second layer over the first layer, and a mask layer over the second layer. A plurality of nano holes are formed through the mask layer and the second layer, wherein the plurality of holes are defined in part by internal surfaces on the second layer and the mask layer. Two or more structure layers are formed on the mask layer and the internal surfaces in the plurality of holes. The two or more structure layers comprise different material compositions.Type: GrantFiled: February 18, 2011Date of Patent: December 4, 2012Assignee: Optotrace (SuZhou) Technologies, Inc.Inventors: Hong Wang, Zhimin Liu