Magnetic Patents (Class 436/526)
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Patent number: 6723237Abstract: The invention relates to a concentration device using magnetic particles and a method therefor aimed at performing concentration of a large volume of liquid efficiently and reliably with a simple structure and on a small device scale. The construction involves having: a liquid suction passage in which liquid can pass through only in a suction direction; a liquid discharge passage in which liquid can pass through only in a discharge direction; a magnetic force device which can exert a magnetic field from outside of the liquid passage on at least one liquid passage thereof or remove the magnetic field, and which can separate magnetic particles having directly or indirectly captured a target substance suspended in the liquid by having the magnetic particles adhere to the inner wall of the liquid passage; a storage section communicated with each liquid passage, for storing the sucked liquid; and a pressure adjustment device for sucking and discharging the liquid by adjusting the pressure in the storage section.Type: GrantFiled: January 11, 2002Date of Patent: April 20, 2004Assignee: Precision Systems Science Co., Ltd.Inventor: Hideji Tajima
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Publication number: 20040072373Abstract: A biochemical labeling material and manufacturing method thereof. The manufacturing method provides a plurality of nanoparticles, bonding the nanoparticles to template molecules by molecular imprinting, polymerizing the nanoparticles to form a matrix with uniformly-distributed template molecules, finally removing the template molecules from the matrix to reveal a detection group of the matrix, leaving a cavity with specific area.Type: ApplicationFiled: June 23, 2003Publication date: April 15, 2004Applicant: INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTEInventors: Chin-I Lin, Kang-Ping Lin, Yuh-Jiuan Lin, Sheng-Ming Shih, Abraham Joseph K, Chao-Kang Chang
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Patent number: 6720192Abstract: A method for assaying an analyte in a sample. The method comprising the steps of a) contacting the sample with material comprising a receptor which is present in a liposome and which liposome comprises a detectable functionality, said contact occurring under conditions resulting in binding of the receptor to analyte if present before or concomitant with step b, wherein step b) consists of contacting the sample with an immobilised ligand for the receptor said contact occurring under conditions resulting in binding of the receptor to the ligand, with steps a and b being followed by c) separating the resulting immobilised ligand-receptor fraction and the receptor fraction present in solution and d) assaying the detectable functionality of the receptor in a fraction from step c) in a manner known per se for its detection.Type: GrantFiled: May 9, 2001Date of Patent: April 13, 2004Assignee: Merska B.V.Inventors: Gerhard Theodoor Viel, Kornelis Ensing
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Publication number: 20040067503Abstract: Silica-coated nanoparticles functionalized with biologically active molecules such as antibodies and nucleotides are used to label cells, to detect and isolate nucleic acid molecules having specific nucleotide sequences, and to separate a mixture of different nucleic acid molecules.Type: ApplicationFiled: April 22, 2003Publication date: April 8, 2004Inventors: Weihong Tan, Jin Shouguang, Xiaojun Zhao, Rovelyn Tapec Dytioco, Timothy James Drake, Lisa Renee Hilliard
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Patent number: 6713271Abstract: Novel magnetic assay methods and systems. According to a preferred embodiment, a chromatographic medium, which preferably comprises a test strip, is provided that is designed to be contacted with a test solution having activated magnetic particles such that the solution flows bilaterally thereacross. A magnetic field, generated by a magnet or electromagnet, is selectively applied to the medium which causes the charged particles to become substantially bound at a site on the medium specified by the position off the magnet, to thus form a captured line or zone. In one preferred embodiment, the magnetic field is applied at the site on the medium at which the test solution is contacted. The degree of magnetic force applied to the membrane may be selectively adjusted to vary the width or surface area of the capture line or zone.Type: GrantFiled: September 25, 2000Date of Patent: March 30, 2004Assignee: Wavesense, LLCInventor: Christopher Feistel
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Publication number: 20040058458Abstract: The present invention relates to modulated (e.g., magnetically modulated) chemical sensors. In particular, the present invention relates to particles comprising fluorescent indicator dyes and methods of using such particles. Magnetic fields and/or Brownian motion modulate an optical property of the particle to distinguish it from background signals. The present invention thus provides improved methods of detecting a wide variety of analytes in fluids, fluid samples, cells and tissues.Type: ApplicationFiled: April 18, 2003Publication date: March 25, 2004Applicant: The Regents of the University of MichiganInventors: Jeffrey Anker, Eric Monson, Raoul Kopelman, Martin Philbert
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Publication number: 20040058387Abstract: When insoluble magnetic carrier particles are used in immunoassay, a method is provided which is suitable for saving labor and for treating a large number of samples within a short time while avoiding problems including difficulties in the stability of sensitized insoluble magnetic particles and in their preparation. In assaying an antigenic substance in test samples, no use is made of insoluble magnetic particles carrying an antibody specific to the said antigenic substance but insoluble magnetic carrier particles are provided in a state free from adsorbing said antibody, etc. Then the antigenic substance per se to be assayed is adsorbed on the insoluble magnetic carrier particles followed by reaction with a labeled antibody specific to the said adsorbed antigenic substance. Thus, the antigenic substance in the test sample can be efficiently assayed in a mode suitable for automation.Type: ApplicationFiled: July 15, 2003Publication date: March 25, 2004Inventors: Masaaki Sakurai, Naoki Takanashi, Masanori Oka, Minoru Hirata
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Publication number: 20040053425Abstract: Custom-engineered glucose oxidase fusion proteins, prepared by recombinant DNA techniques, are employed in a chip-based amperometric immunosensor. This on-chip assay provides quantitative measurement of analyte concentration in any fluid, including all body fluids. The system is designed to facilitate ease in swapping of molecular recognition components and can be rapidly adapted to measure the concentration of any peptide or protein for which a monoclonal antibody is available.Type: ApplicationFiled: May 6, 2003Publication date: March 18, 2004Applicant: Baylor College of MedicineInventors: Richard E. Link, Ronald A. Morton, Brian Miles, Michael Simon
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Patent number: 6706394Abstract: The subject invention related to methods and apparatuses for the manufacture of magnetizable carrier particles. In addition the subject invention pertains to particles having one or more of a variety of particle configurations and/or functional features. These geometric particle configurations and/or functional features such as delivering or removing a pay load can be tailored to achieve one or more desired missions. The subject invention also pertains to a method and apparatus for the delivery of particles to target materials, in order to accomplish one or more of a variety of missions. In a specific embodiment of the subject invention, acicular and other particles with a lengthwise dimension that are substantially uniform and homogenous in their geometry are manufactured and provided with magnetizations. In this way, predictable mechanical force responsivity can be achieved when these particles are subjected to an external magnetic field gradient.Type: GrantFiled: May 2, 2001Date of Patent: March 16, 2004Inventors: Manfred R. Kuehnle, Adelheid Kuehnle
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Publication number: 20040043511Abstract: A membrane-based assay device for detecting the presence or quantity of an analyte residing in a test sample is provided. The device utilizes a self-calibrated magnetic binding assay format (e.g., sandwich, competitive, etc.) that includes detection probes capable of generating a detection signal (e.g., fluorescent non-magnetic particles) and calibration probes capable of generating a calibration signal (e.g., fluorescent magnetic particles). The amount of the analyte within the test sample is proportional (e.g., directly or inversely) to the intensity of the detection signal calibrated by the intensity of the calibration signal. It has been discovered that the fluidics-based device of the present invention provides an accurate, inexpensive, and readily controllable method of determining the presence of an analyte in a test sample.Type: ApplicationFiled: August 27, 2002Publication date: March 4, 2004Applicant: Kimberly-Clark Worldwide, Inc.Inventors: Xuedong Song, Rosann Kaylor
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Publication number: 20040043507Abstract: A fluidics-based assay device for detecting the presence or quantity of an analyte residing in a test sample is provided. The device utilizes a self-calibrated magnetic binding assay format (e.g., sandwich, competitive, etc.) that includes detection probes capable of generating a detection signal (e.g., fluorescent non-magnetic particles) and calibration probes capable of generating a calibration signal (e.g., fluorescent magnetic particles). The amount of the analyte within the test sample is proportional (e.g., directly or inversely) to the intensity of the detection signal calibrated by the intensity of the calibration signal. It has been discovered that the fluidics-based device of the present invention provides an accurate, inexpensive, and readily controllable method of determining the presence of an analyte in a test sample.Type: ApplicationFiled: August 27, 2002Publication date: March 4, 2004Applicant: Kimberly-Clark Worldwide, Inc.Inventors: Xuedong Song, Rosann Kaylor, Shawn Feaster, Kaiyuan Yang
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Publication number: 20040043512Abstract: A self-calibrated, magnetic binding assay (e.g., sandwich, competitive, etc.) for detecting the presence or quantity of an analyte residing in a test sample is provided. The magnetic binding assay includes detection probes capable of generating a detection signal (e.g., fluorescent non-magnetic particles) and calibration probes capable of generating calibration signal (e.g., fluorescent magnetic particles). The amount of the analyte within the test sample is proportional to the intensity of the detection signal calibrated by the intensity of the calibration signal.Type: ApplicationFiled: August 27, 2002Publication date: March 4, 2004Applicant: Kimberly-Clark Worldwide, Inc.Inventors: Xuedong Song, Rosann Kaylor
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Patent number: 6699669Abstract: The present invention relates to improved techniques for separating cells, particles and molecules important to medical science and biotechnology because separation is frequently the limiting factor for many biological processes. The apparatus and method of use provides an innovative method for quantitatively separating cells, proteins, or other particles, using multistage, magnetically, electromagnetically assisted separation technology, (“MAGSEP”). The MAGSEP technology provides a separation technology applicable to medical, chemical, cell biology, and biotechnology processes. Moreover, the instant invention relates to a method for separating and isolating mixtures of combinatorial synthesized molecules such that a variety of products are prepared, in groups, possessing diversity in size, length, (molecular weight), and structural elements. These are then analyzed for the ability to bind specifically to an antibody, receptor, or other ligate.Type: GrantFiled: November 6, 2001Date of Patent: March 2, 2004Assignee: Space Hardware Optimization Technology, Inc.Inventors: John C. Vellinger, Paul W. Todd, Kenneth W. Barton, Scott Dunn, Mark S. Deuser
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Patent number: 6696265Abstract: The present invention relates to the substantial elimination of errors attributable to carryover microspheres, doublets, or misclassification of microsphere subsets. The present invention is based upon passing a sufficient minimum number microspheres through the flow analyzer during an assay run.Type: GrantFiled: August 17, 2000Date of Patent: February 24, 2004Assignee: Luminex CorporationInventor: Michael D. Spain
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Publication number: 20040033627Abstract: A method and/or system for detecting substances of interest. In specific embodiments, the invention involves a method and/or system using magnetic beads and easily manufactured electrical circuits to detect chemicals and/or substances of interest. In other embodiments, the invention involves a method and/or system for providing a variety of biologic assays. In further embodiments, the invention includes methods and/or systems for an associated device, referred to herein as a dual split-drain transistor.Type: ApplicationFiled: June 2, 2003Publication date: February 19, 2004Applicant: The Regents of the University of CaliforniaInventors: Turgut Aytur, P. Robert Beatty, Bernhard Boser, Moshiur Anwar, Eva Harris
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Patent number: 6689615Abstract: Approaches are described for separating plasma from whole blood samples and include the use of magnetically attractable particles associated with an agglutinating agent. The magnetically attractable particles bind the cellular components in a whole blood sample. Application of a magnetic field gradient to a container with the blood sample and the magnetically attractable particles draws the particles to the surface of the container near the source of the magnetic field gradient. The plasma can be removed and stored or used for monitoring or detecting analytes in the plasma.Type: GrantFiled: October 4, 2000Date of Patent: February 10, 2004Inventors: James Murto, Michael Salvati
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Publication number: 20040023273Abstract: The present invention relates to a method of transporting an analyte present in a sample, to a method of concentrating an analyte present in a sample, and to a device for implementing these methods.Type: ApplicationFiled: May 8, 2003Publication date: February 5, 2004Inventors: Pierre Puget, Patrick Pouteau, Frederic Ginot, Patrice Caillat
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Publication number: 20040023261Abstract: Methods, compositions and articles of manufacture for encoding a cell with semiconductor nanocrystals and/or other fluorophors are provided. The encoded cells can be subjected to functional assays in mixed populations, and an assay result can be determined and associated with individual cells by virtue of their code. The methods are particularly useful in multiplex settings where a plurality of encoded cells are to be assayed. The methods are used in screening methods for G protein coupled receptors (GPCRs), for identifying the ligands and functions of orphan GPCRs, and for screening for modulators of GPCRs. Kits comprising reagents for performing such methods are also provided.Type: ApplicationFiled: February 26, 2003Publication date: February 5, 2004Inventors: Marcel P. Bruchez, R. Hugh Daniels, Jennifer Dias, Larry C. Mattheakis, Jianquan Liu
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Publication number: 20040023222Abstract: Methods, apparatus and compositions for separating a desired or undesired population or subpopulation from a biological sample are disclosed herein. The selection procedure is based on ferromagnetic, dense particles in a preferred size range from about 0.8 to about 1.2 microns. Specific binding agents are bound to the particles that recognize and bind to specific molecules on the targeted population or subpopulation, and the particles are mixed with the sample in such a way as to promote movement of the particles relative to the sample, promoting binding to the targeted population or subpopulation without non-specifically binding to non-targeted populations in the sample. Because of the large particle density, the bound population is separated from the fluid sample by gravity.Type: ApplicationFiled: July 31, 2002Publication date: February 5, 2004Inventors: Thomas R. Russell, Pauline McGann, Mike Musick, Michael Ciocci
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Patent number: 6686169Abstract: Reagents and methods for the detection of Staphylococcus aureus are provided. The reagents contain an antibody that binds to a capsular polysaccharide of type 5 of Staphylococcus aureus, and can be used in methods for detection of oxacillin resistant Staphylococcus aureus that escapes detection by agglutination in the presence of fibrinogen and antibodies directed against protein A of Staphylococcus.Type: GrantFiled: August 7, 2002Date of Patent: February 3, 2004Assignee: Institut PasteurInventors: Jean-Michel Fournier, Alain Boutonnier
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Publication number: 20040018644Abstract: Magnetic field sensors, each generating an electrical output signal in proportion to the local magnetic field, are lithographically fabricated on a semiconductor substrate with a small spatial separation. The lateral dimension of the sensors and the separation length are the order of the minimum lithographic feature size. Comparing the electrical signals of the sensors results in a measurement of the local magnetic field gradient. Large field gradients, that vary on a small spatial scale, may be associated small magnetic structures such as microscopic magnetic particles. Detection of a field gradient can be used to infer the presence of a magnetic particle.Type: ApplicationFiled: July 29, 2002Publication date: January 29, 2004Inventors: Mark B. Johnson, Michael Miller
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Patent number: 6682940Abstract: A method of single parameter and multiparameter characterizing of cells, particularly immunophenotyping of cells, is provided. The method preferably uses antibody-coated microspheres which are adapted to bind to specific types of cells. One or more sets of coated microspheres are contacted simultaneously or sequentially with a suspension of cells and bind the cells they are adapted to bind to form bead-cell complexes. Cells may bind to one or more microspheres. The bead-cell complexes are then separated from the suspension The complexes are preferably stained and then examined to characterize the cells, preferably the cells bound to the microspheres. A method of quantitating a specific cell type is provided. A kit and apparatus for performing the method are also provided.Type: GrantFiled: July 20, 2001Date of Patent: January 27, 2004Inventor: Dan A. Pankowsky
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Publication number: 20040009614Abstract: The present invention relates to magnetic particle separators using micromachined magnetic arrays and more particularly, to magnetic particle separators or manipulators using controlled magnetization on micromachined magnetic arrays for the separation of cells and other biological materials. The present invention also pertains to using such devices for the separation and analysis of biological materials for immunoassays, DNA sequencing, protein analysis, and biochemical detection applications. The present invention can also be viewed as a novel method for fabricating fully integrated permanent magnet components within any microelectromechanical system (“MEMS”) structures. The present invention also provides a magnetic particle separation and manipulation system for rapid separation and accurate manipulation of magnetic particles in two-dimensional electromagnetic arrays, which utilize high throughput biological analyses.Type: ApplicationFiled: August 4, 2003Publication date: January 15, 2004Inventors: Chong H Ahn, Hyoung J Cho, Jin-Woo Choi
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Publication number: 20040005718Abstract: According to the present invention, biopolymers are fixed to a substrate by attaching probe DNA to magnetic beads and fixing the above magnetic beads to the substrate by attracting them using a magnetic attraction.Type: ApplicationFiled: March 25, 2003Publication date: January 8, 2004Applicant: YOKOGAWA ELECTRIC CORPORATIONInventor: Kazuhisa Fukushima
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Patent number: 6673631Abstract: The present invention provides methods for isolating a defined quantity of DNA target material from other substances in a medium. The method may be carried out using a known quantity of a silica-containing solid support, such as silica magnetic particles, having a definable capacity for reversibly binding DNA target material, and DNA target material in excess of the binding capacity of the particles. The methods of the present invention involve forming a complex of the silica magnetic particles and the DNA target material in a mixture of the medium and particles, and separating the complex from the mixture using external magnetic force. The DNA target material may then be eluted from the complex. The quantity of DNA target material eluted may be determined based on a calibration model. The methods of the present invention permit isolation of DNA target material which is within a known quantity range.Type: GrantFiled: August 20, 1999Date of Patent: January 6, 2004Assignee: Promega CorporationInventors: Allan M. Tereba, Rex M. Bitner, Susan C. Koller, Craig E. Smith, Daniel D. Kephart, Steven J. Ekenberg
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Patent number: 6672458Abstract: A system and method for manipulating magnetically responsive particles in a solution to separate nucleic acid molecules from cell components in a cell solution. The system and method employ a device capable of receiving a plurality of tubes, each of which contain respective sample and magnetically responsive particles. The device includes heating and cooling devices to facilitate a lysing step to release the nucleic acid molecules from the cells in the cell solution. The device further includes moveable magnets which can be moved proximate to and away from the tube to hold the magnetically responsive particles to which the nucleic acid molecules become bound, so that the molecule-bound particles can be separated from the remainder of the solution, and washed as appropriate. The system also employs an electromagnet which is capable of demagnetizing the particles to allow the particles to freely mix with solution, such as elution solutions which are used to unbind the molecules from the particles.Type: GrantFiled: May 17, 2001Date of Patent: January 6, 2004Assignee: Becton, Dickinson and CompanyInventors: Timothy Roy Hansen, Bradley Scott Thomas, John Joseph Bianco, Matthew P. Collis
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Publication number: 20040002169Abstract: The present invention is directed to processes of separating, analyzing and/or collecting selected species within a target sample by use of magnetic microspheres including magnetic particles, the magnetic microspheres adapted for attachment to a receptor agent that can subsequently bind to selected species within the target sample. The magnetic microspheres can be sorted into a number of distinct populations, each population with a specific range of magnetic moments and different receptor agents can be attached to each distinct population of magnetic microsphere.Type: ApplicationFiled: November 27, 2001Publication date: January 1, 2004Inventors: Robert H. Kraus, Feng Zhou, John P. Nolan
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Patent number: 6660159Abstract: Apparatuses and methods for separating, immobilizing, and quantifying biological substances from within a fluid medium. Biological substances are observed by employing a vessel having a chamber therein, the vessel comprising a transparent collection wall. A high internal gradient magnetic capture structure may be on the transparent collection wall, magnets create an externally-applied force for transporting magnetically responsive material toward the transparent collection wall. The magnetic capture structure comprises a plurality of ferromagnetic members and has a uniform or non-nonuniform spacing between adjacent members. There may be electrical conductor means supported on the transparent collection wall for enabling electrical manipulation of the biological substances. The chamber has one compartment or a plurality of compartments with differing heights. The chamber may include a porous wall.Type: GrantFiled: May 24, 2001Date of Patent: December 9, 2003Assignee: Immunivest CorporationInventors: Leon W. M. M. Terstappen, Gerald Dolan
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Patent number: 6662113Abstract: A method for determining the physical, chemical, or biological correlation between test samples in group A and test samples in group B. This correlation can be determined by contacting a mixture of test samples selected according to a certain rule from group A with test samples from group B to detect the interaction(s) between them. The number of reactions needed to detect these interaction(s) is greatly reduced by preparing a mixture of test samples in group A according to the principle of binary notation, and thus enables a rapid screening.Type: GrantFiled: November 23, 1999Date of Patent: December 9, 2003Assignee: Fujisawa Pharmaceutical Co., Ltd.Inventors: Nobuyoshi Shimizu, Shuichi Asakawa
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Publication number: 20030224366Abstract: This invention relates to magnetic particles having a glass surface which are substantially spherical. This invention also relates to methods for making them, as well as to suspensions thereof and their uses for the purification of DNA or RNA in particular in automated processes.Type: ApplicationFiled: May 16, 2002Publication date: December 4, 2003Inventors: Kurt Weindel, Michael Riedling, Albert Geiger
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Publication number: 20030219753Abstract: Surface plasmon resonance (SPR) sensor biointerface with a rigid thiol linker layer and/or interaction layer ligand loading with reversible collapse and/or iron oxide nanoparticle sensor response amplification.Type: ApplicationFiled: May 22, 2002Publication date: November 27, 2003Inventors: John G. Quinn, Jerry Elkind
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Publication number: 20030215825Abstract: A method of detecting or quantifying a molecular target in a sample utilizing the molecular interaction between molecular targets, bead-bound probes, and support-bound probes. Laser light or a magnetic sensor may be used to detect the beads after the interaction. The detection of the beads indicates the presence of the molecular target in the sample.Type: ApplicationFiled: November 12, 2002Publication date: November 20, 2003Inventor: Sun-Wing Tong
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Patent number: 6649419Abstract: A method and apparatus for extracting, identifying, and manipulating proteins or peptides from a solution uses paramagnetic beads having a coating with an affinity for the target component. In one embodiment, paramagnetic beads coated with C18 are used to adsorb proteins and peptides. The beads can be used to purify, immobilize and assay antibodies. By cycling the beads, many times greater molar amount of binding partner may be separated from a solution. A magnetic probe is used to capture the beads and transfer the beads to selected processing stages.Type: GrantFiled: November 28, 2000Date of Patent: November 18, 2003Assignee: Large Scale Proteomics Corp.Inventor: N. Leigh Anderson
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Patent number: 6649418Abstract: The present invention provides internally calibrated competitive assays for use on a solid support. Additionally, the invention provides a method of using such assays.Type: GrantFiled: July 30, 2001Date of Patent: November 18, 2003Assignee: Silver Lake Research CorporationInventor: Mark S. Geisberg
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Patent number: 6645431Abstract: In a preferred embodiment, an apparatus for automated magnetic separation of materials in laboratory trays, including: a frame upon an upper surface of which a multiwell laboratory tray may be placed; a base plate on which is mounted a plurality of upstanding magnets disposed below the upper surface; and apparatus to raise the base plate such as to insert the upstanding magnets into interwell spaces in the laboratory tray.Type: GrantFiled: January 22, 2001Date of Patent: November 11, 2003Inventor: Thomas W. Astle
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Patent number: 6645777Abstract: A waveguide probe for the detection of pathogens in a sample which comprises a laser, a first and a second tubes that converge at a point to form a proximal end. A magnet is positioned in the end to configure to focus paramagnetic microspheres attached to antigen/antibody/optically labeled antibody complexes in the field of view. The proximal end is polished to form an aperture.Type: GrantFiled: May 17, 2001Date of Patent: November 11, 2003Assignee: The Board of Governors for Higher Education, State of Rhode Island and Providence PlantationInventors: Stephen Letcher, He Cao, Hazem Baqaen, A. Garth Rand
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Patent number: 6642007Abstract: This invention provides novel methods for monitoring urine for type II collagen fragment using a combination of a capture antibody and a detection antibody, such that type II collagen is distinguished from other collagen fragments.Type: GrantFiled: February 15, 2000Date of Patent: November 4, 2003Assignee: Pfizer Inc.Inventors: Mary J. Saltarelli, Kimberly S. Johnson, Ivan G. Otterness
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Publication number: 20030203507Abstract: Compositions and methods are disclosed which enhance the microscopic observation and analysis of biological entities such as cells, bacteria and viruses by eliminating interfering magnetic clusters created by naturally occurring aggregators of colloidal magnetic particles. Additionally means for significantly enhancing the magnetic isolation of low antigen density target cells from biological samples are disclosed.Type: ApplicationFiled: May 30, 2003Publication date: October 30, 2003Inventors: Paul A. Liberti, Galla Chandra Rao, Leon W.M.M Terstappen
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Patent number: 6635181Abstract: A continuous, hybrid magnetic field gradient device for colloidal magnetic affinity separation having an axially-rotating horizontal glass tube, and a plurality of axially located repeating magnetic units. Each magnetic units consists of an alternating current solenoid that surrounds the chamber followed by computer-controlled electromagnets carrying a direct current. The on-off cycle of the electromagnets is used to control the residence time of target-bound magnetic particles in the chamber thereby allowing the separate collection of the particle and target rich fractions and the target-lean fractions without interrupting the feed flow. The azimuthally flowing alternating current in the solenoid introduces transient axial and radial forces as well as torque on the magnetic particles, promoting mixing.Type: GrantFiled: August 20, 2001Date of Patent: October 21, 2003Assignee: The Board of Governors for Higher Education, State of Rhode Island and Providence PlantationsInventor: Arijit Bose
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Patent number: 6632929Abstract: A covalent conjugate of a 4′-hydroxyazobenzene-2-carboxylic acid derivative (HABA) and an avidin-type molecule, of the formula: wherein A is (CH2)n or —CH═CH—, wherein n is an integer from 0-10; B is (CH2)n wherein n is an integer from 2 to 10; m is zero or 1; and Av is the residue of an avidin-type molecule selected from the group comprising native egg-white avidin, recombinant avidin, deglycosylated avidins, bacterial streptavidin, recombinant streptavidin, truncated streptavidin and other derivatives of said avidin-type molecules. These HABAylated avidins are red colored in the quinone configuration and can be used in many applications in the avidin-biotin technology.Type: GrantFiled: August 7, 2001Date of Patent: October 14, 2003Assignee: Yeda Research and Development Co. LTDInventors: Meir Wilchek, Edward A Bayer, Heike Hofstetter, Margherita Morpurgo
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Patent number: 6630358Abstract: Protein arrays for the parallel, in vitro screening of biomolecular activity are provided. Methods of using the protein arrays are also disclosed. On the arrays, a plurality of different proteins, such as different members of a single protein family, are immobilized on one or more organic thinfilms on the substrate surface. The protein arrays are particularly useful in drug development, proteomics, and clinical diagnostics.Type: GrantFiled: May 12, 2000Date of Patent: October 7, 2003Assignee: Zyomyx, IncorporatedInventors: Peter Wagner, Dana Ault-Riche, Steffen Nock, Christian Itin
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Patent number: 6630316Abstract: A method for measuring the responses of sets or subsets of lymphocytes to mitogens or antigens in a sample is disclosed comprising incubating a population of cells with a mitogen or antigen, separating the desired subset of cells by means of the interaction of a specific binding reagent that is attached to the solid phase with a cell surface determinant that is present on the cell subset of interest, lysing the separated cells, and measuring an intracellular component that is increased if the cells have responded to the stimulus. The method provides a convenient, simple, and reliable method for measuring immune function in a variety of conditions.Type: GrantFiled: April 14, 1998Date of Patent: October 7, 2003Assignee: Cylex, Inc.Inventor: Marjorie L. Wier
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Publication number: 20030186465Abstract: The present invention provides a particle identification apparatus including a flow cell for passage of fluid containing a population of labeled magnetic microspheres in a stream, the magnetic microspheres having a label providing a detectable property to the magnetic microspheres, and, a magnetic measurement system, positioned adjacent to the flow cell, for measuring a magnetic moment on each labeled magnetic microsphere as it passes by the magnetic measurement system.Type: ApplicationFiled: November 27, 2001Publication date: October 2, 2003Inventors: Robert H. Kraus, Feng Zhou, John Nolan
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Publication number: 20030178309Abstract: This invention relates generally to the field of moiety or molecule isolation, detection and manipulation and library synthesis. In particular, the invention provides a bead, which bead comprises: a) a magnetizable substance; and b) an electrically conductive substance or an optical labeling substance. Methods and kits for isolating, detecting and manipulating moieties and synthesizing libraries using the beads are also provided.Type: ApplicationFiled: March 21, 2002Publication date: September 25, 2003Inventors: Mingxian Huang, Lei Wu, Xiaobo Wang, Junquan Xu, Guo Liang Tao, Jing Cheng
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Patent number: 6623984Abstract: The present invention provides a MEMS-based integrated particle identification system having a substrate, a magnetic structure, and a bioferrograph. The substrate includes a topside portion, backside portion and a flow system. The flow system includes a flow channel for accepting the flow of a stream of particles to identified. The magnetic structure is in physical communication with the topside and backside portions of the substrate and has at least two pole pieces. A plurality of pole piece embodiments are provided for generating a magnetic field that acts on magnetically susceptible particles in the flow stream. The bioferrograph has at least one sensor for identifying the presence and quantity of magnetically susceptible particles. A plurality of sensor embodiments are also provided.Type: GrantFiled: November 1, 2000Date of Patent: September 23, 2003Assignee: The Cleveland Clinic FoundationInventors: Aaron J. Fleischman, Shuvo Roy, Jeff Chalmers, Maciej Zborowski
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Patent number: 6623982Abstract: Compositions and methods are disclosed which enhance the microscopic observation and analysis of biological entities such as cells, bacteria and viruses by eliminating interfering magnetic clusters created by naturally occurring aggregators of colloidal magnetic particles. Additionally means for significantly enhancing the magnetic isolation of low antigen density target cells from biological samples are disclosed.Type: GrantFiled: July 12, 1999Date of Patent: September 23, 2003Assignee: Immunivest CorporationInventors: Paul A. Liberti, Galla Candra Rao, Leon W. M. M. Terstappen
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Patent number: 6623983Abstract: Apparatus and methods are disclosed which facilitate immobilization of magnetically labelled particulate entities, e.g., cells, preferably in a defined pattern, on a collection surface via binding between specific binding pair members, as an aid to particle analysis.Type: GrantFiled: September 23, 1999Date of Patent: September 23, 2003Assignee: Immunivest CorporationInventors: Leon W. M. M. Terstappen, Galla C. Rao
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Patent number: 6620627Abstract: Compositions and methods are disclosed which enhance the microscopic observation and analysis of biological entities such as cells, bacteria and viruses by eliminating interfering magnetic clusters created by naturally occurring aggregators of colloidal magnetic particles. Additionally means for significantly enhancing the magnetic isolation of low antigen density target cells from biological samples are disclosed.Type: GrantFiled: October 31, 2000Date of Patent: September 16, 2003Assignee: Immunivest CorporationInventors: Paul A. Liberti, Galla Candra Rao, Leon W. M. M. Terstappen
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Patent number: 6613582Abstract: The present application describes novel uses of ruthenium bipyridyls or palladium porphyrins as photo-activatable crosslinking agents. Crosslinking can be between any two molecules including peptides, proteins, or compounds. Crosslinking occurs in the presence of an electron donor such as ammonium persulfate, and requires only moderate intensity visible light. Crosslinking can be between peptides, polypeptides or lead candidate compounds to unknown target molecules. Reagents utilyzing ruthenium bipyridyls and palladium porphyrins crosslinkers for use in diagnostic and detection scenarios are also disclosed.Type: GrantFiled: May 25, 2000Date of Patent: September 2, 2003Assignee: Board of Regents, The University of Texas SystemInventors: Thomas J. Kodadek, David A. Fancy, Stephen A. Johnston
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Patent number: 6607891Abstract: A pretreatment method for assaying a substance which comprises mixing a biological specimen with at least one pretreating agent selected from among surfactants and alkali agents, thus releasing binding proteins in the biological specimen from the substance to be assayed and, at the same time, inactivating the proteins by irreversible denaturation to thereby eliminate the effects of the binding proteins coexisting in the biological specimen.Type: GrantFiled: January 31, 2001Date of Patent: August 19, 2003Assignee: Mitsubishi Chemical CorporationInventor: Makoto Takada