Magnetic Patents (Class 436/526)
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Patent number: 7527978Abstract: The invention is a method for analyzing immature reticulocytes for the presence of micronuclei. The method includes reticulocyte enrichment, fluorescent labeling, micronuclei staining, and analysis using single-laser flow cytometry. The invention also includes kits containing reagents to use in the method.Type: GrantFiled: October 7, 2004Date of Patent: May 5, 2009Assignee: Children's Hospital & Research Center at OaklandInventors: Tal Offer, Emily Ho, Bruce N. Ames, Frans Kuypers
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Patent number: 7527980Abstract: Assay systems and methods are provided for detecting a target antigen in a physiological fluid (e.g., blood, serum, or urine). The method includes linking via a first antibody a magnetic microparticle to the target antigen in the physiological fluid; linking via a second antibody a glucose molecule to the target antigen; utilizing a magnetic field to separate the magnetic microparticle-linked antigen from the physiological fluid to form a test sample; and detecting the glucose in the test sample to determine the concentration of target antigen in the physiological fluid. The target antigen can be a protein or marker resulting from cardiac tissue injury, which can be used to assess acute myocardial infarction. An exemplary target antigen is myoglobin. The glucose detection preferably is one that can be done rapidly, e.g., with a conventional glucometer, and may include measuring the electrical resistance, color, or pH of the test sample.Type: GrantFiled: August 16, 2005Date of Patent: May 5, 2009Assignee: Florida State University Research FoundationInventor: Yousef Haik
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Patent number: 7527979Abstract: Assay systems and methods are provided for detecting a target pathogen, such as a microorganism (e.g., bacterium, bacterial toxin) which may be present in a fluid or other location. The method can include linking a magnetic microparticle to a first epitope of the target microorganism in a fluid via a first antibody; utilizing a magnetic field to separate the magnetic microparticle and linked targeted microorganism from at least a portion of other components in the fluid, thereby forming a test sample; linking a glucose molecule to a second epitope of the target microorganism via a second antibody; and detecting the glucose in the test sample to determine the presence or concentration of the target microorganism in the fluid. The glucose detection preferably is one that can be done rapidly, e.g., with a conventional glucometer, and may include measuring the electrical resistance, color, or pH of the test sample.Type: GrantFiled: August 16, 2005Date of Patent: May 5, 2009Assignee: Florida State University Research FoundationInventor: Yousef Haik
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Patent number: 7524630Abstract: Silica-coated nanoparticles functionalized with biologically active molecules such as antibodies and nucleotides are used to label cells, to detect and isolate nucleic acid molecules having specific nucleotide sequences, and to separate a mixture of different nucleic acid molecules.Type: GrantFiled: April 22, 2003Date of Patent: April 28, 2009Assignee: University of Florida Research Foundation, Inc.Inventors: Weihong Tan, Jin Shouguang, Xiaojun Zhao, Rovelyn Tapec Dytioco, Timothy James Drake, Lisa Renee Hilliard
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Patent number: 7514270Abstract: An analytical instrument for the immobilization of antigens to be detected in an immunoassay using mangetizable beads. The instrument includes a probe containing magnetizable particles and a plurality of magnetic devices for applying a magnetic field gradient to the particles in the probe reservoir. The probe is movable between the plurality of magnetic devices and at least one of the probe positions may be used to immobilize the magnetic particles in the fluid medium and at least another position may be used to resuspend the particles in a fluid medium.Type: GrantFiled: April 10, 2003Date of Patent: April 7, 2009Assignee: Instrumentation Laboratory CompanyInventors: Eugene K. Achter, Hartmut Richard Schroeder, Stephen S. Trotta
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Patent number: 7510839Abstract: Embodiments generally relate to a PCR wherein a magnetic field is focused in a localized area and also to the possibility of a cyclic transfer of heat. According to at least one embodiment of the invention, a magnetic field gradient is constructed along the direction of flow and the transfer of heat is carried out in a manner perpendicular to the direction of flow. Suitable things are provided therefore. The magnetic field is, in particular, focused by Mumetal® which is arranged on both sides of the flow channel and the temperature is controlled by way of Peltier elements, cooling bodies, and thermal coupling plates.Type: GrantFiled: October 14, 2005Date of Patent: March 31, 2009Assignee: Siemens AktiengesellschaftInventors: Walter Gumbrecht, Peter Paulicka, Manfred Stanzel
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Patent number: 7507588Abstract: Arrays of microparticle populations, each population labeled with a single fluorescent dye, are provided for use in multiplex assays. The populations form a virtual multidimensional array wherein each microparticle is identified by fluorescence intensity in two different fluorescence detection channels. The arrays are useful in a variety of assays, including multiplex, multi-analyte assays for the simultaneous detection of two or more analytes by, for example, flow cytometry, and a labeling reagents in, for example, microscopy. The use of singly-dyed microparticles to form multidimensional arrays greatly simplifies the creation of multiplex assays.Type: GrantFiled: April 14, 2006Date of Patent: March 24, 2009Assignee: Becton, Dickinson and CompanyInventors: Majid Mehrpouyan, Diether J. Recktenwald, Rudolf Varro
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Patent number: 7504262Abstract: The present invention generally relates to the field of biomolecule detection. More specifically, the present invention relates to compositions, methods and systems for the detection and manipulation of biomolecules using magnetic particles.Type: GrantFiled: February 23, 2006Date of Patent: March 17, 2009Inventor: John Fox
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Patent number: 7494782Abstract: A method for determining the concentration of factor VIIa-antithrombin complexes is disclosed which has application to estimating the level of intravascular exposure of tissue factor, assessing patient risk for hypercoagulation or other coagulopathies, and monitoring patients for factor VIIa-antithrombin complexes over time which can reveal changes in risk for hypercoagulation or other coagulopathies and/or effectiveness of anticoagulant therapy. Antibodies suitable for use in an in vitro assay for determining the concentration of factor VIIa-antithrombin complexes and methods for making the same are also disclosed.Type: GrantFiled: July 2, 2002Date of Patent: February 24, 2009Inventor: James H. Morrissey
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Publication number: 20090041776Abstract: The invention as described herein provides compositions and methods for cancer immunotherapy and cancer detection. In particular, the invention discloses plant-derived human monoclonal antibodies that bind human carcinoma antigens in cancer cell lines.Type: ApplicationFiled: April 23, 2008Publication date: February 12, 2009Applicants: Biotechnology Foundation, Inc., The Chancellor, Masters and Scholars of the University of Oxford, University of OxfordInventors: Hilary Koprowski, Kisung Ko, Pauline Rudd, Yoram Tekoah, Raymond Dwek
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Patent number: 7486166Abstract: The present disclosure provides a high performance hybrid magnetic structure made from a combination of permanent magnets and ferromagnetic pole materials which are assembled in a predetermined array. The hybrid magnetic structure provides means for separation and other biotechnology applications involving holding, manipulation, or separation of magnetic or magnetizable molecular structures and targets. Also disclosed are further improvements to aspects of the hybrid magnetic structure, including additional elements and for adapting the use of the hybrid magnetic structure for use in biotechnology and high throughput processes.Type: GrantFiled: November 9, 2006Date of Patent: February 3, 2009Assignee: The Regents of the University of CaliforniaInventors: David E. Humphries, Martin J. Pollard, Christopher J. Elkin
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Publication number: 20090023159Abstract: The invention relates to a method for the in vitro diagnosis of Alzheimer's disease using a monoclonal antibody. Said antibody can bind at least to amino acids 12-16 of the ?-amyloid peptide, specifically detecting the neuritic plaques which are characteristic of Alzheimer's disease, without detecting diffuse plaques which are not defining characteristics of the disease. Within the neuritic plaques, the monoclonal antibody can detect a different sub-group in the composition of the different deposited isoforms of the ?-amyloid peptide, which is associated with the disease progression stage. In addition, the antibody can bind to isoforms of the ?-amyloid peptide in biological fluids such as urine. As a result, the inventive monoclonal antibody, the cell lines that produce said antibody and compositions containing same can be used in the in vitro diagnosis of Alzheimer's disease and in determining the disease progression stage.Type: ApplicationFiled: March 9, 2006Publication date: January 22, 2009Inventor: Enrique Mendez
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Patent number: 7476313Abstract: An apparatus and method for carrying out the affinity separation of a target substance from a liquid test medium by mixing magnetic particles having surface immobilized ligand or receptor within the test medium to promote an affinity binding reaction between the ligand and the target substance. The test medium with the magnetic particles in a suitable container is removably mounted in an apparatus that creates a magnetic field gradient in the test medium. This magnetic gradient is used to induce the magnetic particles to move, thereby effecting mixing. The mixing is achieved either by movement of a magnet relative to a stationary container or movement of the container relative to a stationary magnet. In either case, the magnetic particles experience a continuous angular position change with the magnet.Type: GrantFiled: May 17, 2006Date of Patent: January 13, 2009Inventor: Iqbal Waheed Siddiqi
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Patent number: 7474184Abstract: The present disclosure provides a high performance hybrid magnetic structure made from a combination of permanent magnets and ferromagnetic pole materials which are assembled in a predetermined array. The hybrid magnetic structure provides means for separation and other biotechnology applications involving holding, manipulation, or separation of magnetizable molecular structures and targets. Also disclosed are hybrid magnetic tweezers able to exert approximately 1 nN of force to 4.5 ?m magnetic bead. The maximum force was experimentally measured to be ˜900 pN which is in good agreement with theoretical estimations and other measurements. In addition, a new analysis scheme that permits fast real-time position measurement in typical geometry of magnetic tweezers has been developed and described in detail.Type: GrantFiled: February 15, 2006Date of Patent: January 6, 2009Assignee: The Regents of the University of CaliforniaInventors: David E. Humphries, Seok-Cheol Hong, Linda A. Cozzarelli, legal representative, Martin J. Pollard, Nicholas R. Cozzarelli
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Publication number: 20090004758Abstract: Embodiments of the invention relate generally to ferromagnetic microdisks, methods of detecting target bioanalyte using ferromagnetic microdisks, and kits (such as for using in the laboratory setting) containing the reagents necessary to make, and/or use ferromagnetic microdisks for bioanalyte detection, depending on the user's planned application. The methods and products allow the fabrication of ferromagnetic microdisks, and their use in the detection of biological molecules with high sensitivity, little or no signal decay, improved safety, convenience, and lowered cost for use and disposal.Type: ApplicationFiled: June 29, 2007Publication date: January 1, 2009Inventor: Chang-Min Park
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Patent number: 7470397Abstract: The invention provides disintegratable film compositions for diagnostic test devices. The films are prepared with a combination of components that yield films of sufficient film strength and desired disintegration profiles. A disintegratable film according to the present invention contains a water soluble high molecular weight component, a water soluble low molecular weight component, and one or more reagents for use in a diagnostic device. Optionally, the films further contain a starch component, a glucose component, a plasticizer and/or a humectant, and/or a filler. The invention further provides a diagnostic testing device, which includes a film according to an embodiment of the invention, and methods of using such devices.Type: GrantFiled: October 22, 2004Date of Patent: December 30, 2008Assignee: Adhesives Research, Inc.Inventors: William G. Meathrel, Nathan A. Meyer, Scott D. Barnhart, Cathy M. Moritz, Andrew P. Full, Susan R. Newsom, Mary Robertson
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Publication number: 20080318342Abstract: An apparatus (10) and method for use in a vertical flow-through assay process is characterised by applying pressure to a sample to force the sample through a reaction/capture membrane (12), to which one or more ligands are bound, at a controlled rate. Typically, the method includes a pre-incubation step in which the sample and a detection analyte typically an antibody bound to colloidal gold or a fluorescent tag bind together The pre-incubation step typically takes place in a chamber (26) spaced above the capture membrane (12). The base of the chamber is defined by a porous hydrophobic frit (34) typically formed from polyethylene. It is preferred that the chamber (26) is defined by the upper part of a cylinder (24) extending from a seal (30) compressing the reaction membrane (12) against an absorbent pad (28). The seal (30) has the effect of compressing tie reaction membrane and preventing wicking of the sample in the lateral direction outside of the circular seal.Type: ApplicationFiled: June 6, 2005Publication date: December 25, 2008Inventors: Matthew C. A. Durack, Rowan Buls
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Publication number: 20080311677Abstract: A method of detecting a target substance includes contacting the target substance with a substrate. The substrate has a first receptor bound to the substrate. The target substance binds to the first receptor. The method further includes contacting a second receptor with the substrate. The second receptor is associated with the target substance. The second receptor is biotinylated. The method also includes contacting an anti-biotin antibody conjugated paramagnetic particle with the substrate.Type: ApplicationFiled: March 12, 2008Publication date: December 18, 2008Applicant: GENEXPRESS INFORMATICSInventors: Robert C. Chin, Gregory F. Lopreato
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Publication number: 20080311678Abstract: The present invention is to present a sample analyzer which is capable of separate and dispose of the reaction container and the reaction liquid respectively even when magnetic particles are included in a reagent. The sample analyzer 1 comprises: a detector 16 for detecting the target substance by using a cuvette 300 which contains the target substance captured by the magnetic particle; an adsorption section 183 for magnetically adsorbing the magnetic particle in the cuvette 300; a liquid aspiration section 18 for aspirating a liquid in the cuvette 300 in a state in which the magnetic particle in the cuvette 300 is magnetically adsorbed by the adsorption section 183; and a disposal section 19 for accommodating the cuvette 300.Type: ApplicationFiled: June 12, 2008Publication date: December 18, 2008Applicant: SYSMEX CORPORATIONInventors: Toshihiro OOTANI, Kazunori MOTOTSU, Miyuki MATSUE, Jun MATSUI, Hironori KATSUMI
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Patent number: 7465538Abstract: Heterogeneous assays for different analytes in a single biological sample are performed simultaneously in a multiplexed assay that combines flow cytometry with the use of magnetic particles as the solid phase and yields an individual result for each analyte. The particles are distinguishable from each other by characteristics that permit them to be differentiated into groups, each group carrying an assay reagent bonded to the particle surface that is distinct from the assay reagents of particles in other groups. The magnetic particles facilitate separation of the solid and liquid phases, permitting the assays to be performed by automated equipment. Assays are also disclosed for the simultaneous detection of antibodies of different classes and a common antigen specificity or of a common class and different antigen specificities.Type: GrantFiled: August 9, 2006Date of Patent: December 16, 2008Assignee: Bio-Rad Laboratories, Inc.Inventors: Michael I. Watkins, Richard B. Edwards
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Patent number: 7462322Abstract: An apparatus for performing a binding assay for an analyte of interest present in a sample based upon measurement of electrochemiluminescence at an electrode surface comprising a cell defining a sample containing volume intersecting with inlet and outlet means, an electrode having a substantially horizontally positioned surface exposed to and positioned below a portion of the sample containing volume, means for impressing electrochemical energy upon said electrode sufficient to generate luminescence, means for magnetically collecting particles along said surface and means for measuring the luminescence generated at said electrode.Type: GrantFiled: August 6, 2004Date of Patent: December 9, 2008Assignee: Bioveris CorporationInventors: John K. Leland, Haresh P. Shah, John H. Kenten, Jack E. Goodman, George E. Lowke, Yuzaburo Namba, Gary F. Blackburn, Richard J. Massey
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Patent number: 7445895Abstract: The present invention relates to methods, kits and assay system for detecting drug-resistant Mycobacterium tuberculosis of suspected patient. The system of the present invention largely reduces the whole process of drug-resistant Mycobacterium tuberculosis detection to less than 5 hours.Type: GrantFiled: April 28, 2004Date of Patent: November 4, 2008Assignee: AsiaGEN CorporationInventors: George Chin-Sheng Chou, Chuang-Yi Huang
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Publication number: 20080268481Abstract: The invention relates to a sensor device for detecting a target in a sample suspected of comprising the target, which device is suitable for use in an assay such as a sandwich assay. The invention further relates to a method for detecting a target in a sample. The device comprises a sensor surface, which is functionalised with one moiety of a strong binding couple, which moiety preferably shows little or no cross reactivity with the target molecule.Type: ApplicationFiled: November 21, 2006Publication date: October 30, 2008Applicant: KONINKLIJKE PHILIPS ELECTRONICS, N.V.Inventors: Menno Willem Jose Prins, Wendy Uyen Dittmer
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Publication number: 20080261329Abstract: Technical field: analysis of mixtures, predominantly of biologic origin, for the contents of biological and/or chemical components, and analysis of mixtures, which parameters determine vital functions of biologic objects. Entity: spatially arranging a chosen component attached to magnetic particles, exposing the particles to a magnetic field, and recording a magnetic induction signal, from which the content of the analyte in the mixture is judged; this includes grouping the chosen component in a probe volume, making the magnetic field alternating, pre-setting its spectrum, at least, at two frequencies, and recording the signal at a frequency, which is a linear combination of these frequencies, during the exposure of the magnetic particles to the field.Type: ApplicationFiled: April 8, 2008Publication date: October 23, 2008Inventors: Petr Ivanovich Nikitin, Petr Mikhailovich Vetoshko
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Patent number: 7432046Abstract: The present invention relates to methods of determining anti-infectious agent IgG avidity, for example, human anti-cytomegalovirus and human anti-toxoplasma IgG avidity, using a competitive assay format.Type: GrantFiled: November 2, 2005Date of Patent: October 7, 2008Assignee: Abbott LaboratoriesInventors: Gregory T. Maine, Stephen C. Hsu, Darwin D. Smith, Dominick L. Pucci, Jörg Herzogenrath, Ingo Curdt, Heike Maria Christ
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Patent number: 7432105Abstract: A self-calibrated, magnetic binding assay (e.g., sandwich, competitive, etc.) for detecting the presence or quantity of an analyte residing in a test sample is provided. The magnetic binding assay includes detection probes capable of generating a detection signal (e.g., fluorescent non-magnetic particles) and calibration probes capable of generating calibration signal (e.g., fluorescent magnetic particles). The amount of the analyte within the test sample is proportional to the intensity of the detection signal calibrated by the intensity of the calibration signal.Type: GrantFiled: August 27, 2002Date of Patent: October 7, 2008Assignee: Kimberly-Clark Worldwide, Inc.Inventors: Xuedong Song, Rosann Kaylor
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Patent number: 7431887Abstract: System and method for providing a sample preparing arrangement (10) submergible in a liquid medium that includes a carrier structure (11) having at least one cavity (12) therein and that is in communication with an arrangement (13) for controllable generation of a magnetic field through influence of a control signal. The sample preparing arrangement includes a magnetic covering structure (14) for covering and/or uncovering the cavity in operative interaction with the magnetic field.Type: GrantFiled: February 24, 2003Date of Patent: October 7, 2008Inventors: David Storek, Niklaus Schneeberger, Britta Ottosson, Anafol Krozer, Robert P Otillar, Jr.
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Publication number: 20080241964Abstract: The present invention relates to a capture agent member being used in a magnetic sensor to detect the presence or concentration of a target substance in a test solution by detecting the presence or number of the magnetic marker, wherein the capture agent member contains a capture agent for capturing the target substance and a labeling agent serving as a nucleus for the magnetic marker to agglutinate, and wherein the capture agent is labeled with the labeling agent. The present invention relates to a material which improves detection sensitivity of a biosensor using a magnetic sensor while maintaining reactivity and dispersibility. In particular, according to the present invention, there can be provided a material which enables realization of high sensitivity by using a simple magnetic biosensor such as a semiconductor Hall element and a magnetoresistance effect element.Type: ApplicationFiled: March 24, 2008Publication date: October 2, 2008Applicant: CANON KABUSHIKI KAISHAInventors: Masaru Kaieda, Takashi Ikeda, Kazumichi Nakahama
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Patent number: 7427512Abstract: A method and apparatus for the manipulation of colloidal particulates and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled electrokinetic assembly of particles near surfaces relics on the combination of three functional elements: the AC electric field-induced assembly of planar aggregates; the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The present invention provides a set of fundamental operations enabling interactive control over the creation and placement of planar arrays of several types of particles and biomolecules and the manipulation of array shape and size. The preset invention enables sample preparation and handling for diagnostic assays and biochemical analysis in an array format, and the functional integration of these operations.Type: GrantFiled: August 3, 2004Date of Patent: September 23, 2008Assignee: BioArray Solutions Ltd.Inventor: Michael Seul
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Patent number: 7425455Abstract: A biosensor not requiring washing of an unbonded label molecular by analyzing an object to be measured such as an antigen, an antibody, a DNA, and an RNA through magnetic field sensing. The biosensor is small in size, low in price, and high in sensing accuracy. Semiconductor Hall devices are arrayed two-dimensionally on the bottoms of recesses in the surface of a sensor chip to which bonded is a magnetic molecule with which a magnetic particle is labeled so as to sense the magnetic field produced along the sensor surface of the sensor chip. The surface area of each semiconductor Hall device is less than the maximum cross section of the magnetic molecule, and the intervals of the arrayed semiconductor Hall devices are larger than the diameter of the magnetic Hall molecule. Thus, the analysis accuracy is enhanced. An interconnection is used commonly to the semiconductor Hall devices, thereby reducing the size of the sensor.Type: GrantFiled: January 29, 2003Date of Patent: September 16, 2008Assignee: Asahi Kasei Kabushiki KaishaInventors: Hirofumi Fukumoto, Masayuki Nomura
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Publication number: 20080206892Abstract: The invention provides a method, a device and a system for determining a concentration of at least one sort of polarizable or polarized magnetic labels in a fluid, the sensing surface comprising at least one sort of binding sites capable of specifically attaching to at least one sort of biological entities linked to the magnetic labels, the sensing device further comprising at least one magnetic sensor element, the sensing device further comprising first means for determining the concentration of magnetic labels attached to the binding sites and second means for determining the time of arrival of the fluid.Type: ApplicationFiled: June 14, 2006Publication date: August 28, 2008Applicant: KONINKLIJKE PHILIPS ELECTRONICS, N.V.Inventor: Menno Wille Jose Prins
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Publication number: 20080206891Abstract: The present invention provides a synthetic antiferromagnetic (SAF) nanoparticle. The SAF nanoparticle includes at least two ferromagnetic layers and at least one non-magnetic spacer layer. The spacer layer is situated in between planar surfaces of the ferromagnetic layers. The saturation field of the SAF nanoparticle is tunable by the geometry and composition of the nanoparticle. Preferably, the saturation field can be tuned to be between about 100 Oe and about 10,000 Oe. Also preferably, the SAF nanoparticle has a magnetic moment of at least 800 emu/cm3. In a preferred embodiment, the SAF nanoparticle has at least one of a biomolecule, a recognition moiety, or a molecular coating attached to its surface. The SAF nanoparticle may also have a dye attached to its surface.Type: ApplicationFiled: August 16, 2007Publication date: August 28, 2008Inventors: Shan X. Wang, Robert John Wilson, Wei Hu
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Patent number: 7410811Abstract: The present invention relates to an analytical method and device utilizing magnetic materials and acoustics sensor. The invention employs a magnetic material modified with specific recognizable molecules to capture a substance in a sample and provides an external magnetic field to draw the magnetic material to an sensing region of an acoustics sensor, and then converts an effect occurred on a surface of the sensing region to an amount of the substance in the sample, wherein the effect is caused by a contact of the surface with the magnetic material and the substance.Type: GrantFiled: December 30, 2005Date of Patent: August 12, 2008Assignee: Industrial Technology Research InstituteInventors: Yuh Jiuan Lin, Kun Feng Lee, Chi Min Chau, Hui Ju Cho
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Publication number: 20080182341Abstract: The present invention is related to compositions useful for the measurement of free or unbound analyte concentrations in a fluid. The present invention includes the use of capture ligands and stabilizing agents to improve the accuracy of analyte concentration assays. Methods and tools for using the present invention are also disclosed.Type: ApplicationFiled: July 20, 2007Publication date: July 31, 2008Inventors: Wenyuan Lu, Katherine M. Leith, Stephen P. Chan, Courtney E. Walton
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Publication number: 20080176254Abstract: The present invention provides compositions and methods for affinity separation of targets from mixtures. In particular, disclosed are avian IgY antibodies coupled to solid supports and their methods of use.Type: ApplicationFiled: October 18, 2007Publication date: July 24, 2008Inventors: Xiangming Fang, Lei Huang, Ping Lin, Jerald S. Feitelson, Wei-Wei Zhang
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Patent number: 7402381Abstract: Aspects of the invention can provide a method capable of easily realizing immobilization with the optimum density derived from a concentration control and without phase separation in coadsorption of a number of molecules. The immobilization method can include the step of dissolving a plurality of molecules to be immobilized to a solid phase substrate with a solvent to obtain a solution of the plurality of molecules, and the step of incubating the solution and the solid phase substrate in touch therewith. Each of the molecules can include a solid phase substrate joint portion having a jointing property to the solid phase substrate, a functional portion having a specific function, and a linker portion positioned between the solid phase substrate joint portion and the functional portion.Type: GrantFiled: August 27, 2004Date of Patent: July 22, 2008Assignee: Seiko Epson CorporationInventors: Hiroshi Takiguchi, Hitoshi Fukushima
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Publication number: 20080171400Abstract: A magnetic microparticle-packing unit using centrifugal force, a microfluidic device including the same, and an immunoassay method using the microfluidic device are provided. The magnetic microparticle-packing unit includes a rotary body controllably rotating; a microfluidic channel which includes a curved portion in which the microfluidic channel first extends away from the rotation center of the rotary body and then turns toward the rotation center of the rotary body.Type: ApplicationFiled: August 15, 2007Publication date: July 17, 2008Applicant: SAMSUNG ELECTRONICS CO., LTDInventors: Yoon-kyoung CHO, Beom-seok LEE, Jeong-gun LEE
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Patent number: 7396690Abstract: A method for treating magnetic particles present in a solution in a container by redispersion, rinsing or displacement, the particles having novel characteristics under the effect of a specific magnet and being associated or not with biological entities. The magnetic particles undergo at least one low-intensity magnetization where they are disposed in filaments oriented according to the north-south axis of the magnet; the magnetization source and/or container is/are displaced while the magnetic effect is maintained on the magnetic particles; the magnetization source and/or container is/are displaced or the, magnetization is stopped, in order to suppress the magnetic field on the magnetic particles. Preferably, the invention can be used in the field of biology.Type: GrantFiled: July 9, 2002Date of Patent: July 8, 2008Assignee: BioMerieux S.A.Inventor: Bruno Colin
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Publication number: 20080160634Abstract: An embodiment of the invention relates to a device for detecting an analyte in a sample. The device comprises a fluidic network and an integrated circuitry component. The fluidic network comprises multiple zones such as a sample zone, a cleaning zone and a detection zone. The fluidic network contains a magnetic particle and/or a signal particle. A sample containing an analyte is introduced, and the analyte interacts with the magnetic particle and/or the signal particle through affinity agents. A microcoil array or a mechanically movable permanent magnet is functionally coupled to the fluidic network, which are activatable to generate a magnetic field within a portion of the fluidic network, and move the magnetic particle from the sample zone to the detection zone. A detection element is present which detects optical or electrical signals from the signal particle, thus indicating the presence of the analyte.Type: ApplicationFiled: December 28, 2006Publication date: July 3, 2008Inventors: Xing Su, Kai Wu, David J. Liu
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Publication number: 20080160537Abstract: The present invention provides a method for detecting the presence or amount of an analyte in a sample, said method comprising: a porous surface to which particles having attached thereto a binding substance, analyte and binding substance coated label particles are added. If said analyte is present in the sample an immuno- or chemical reaction occurs in the liquid phase. The separation of bound complex from unbound material is achieved by using said surface where the separation occurs mainly two dimensionally on the surface of the porous surface (e.g. grid). Interestingly, the disclosed surface enables a separation where said complexes are distributed two dimensionally on the porous surface (e.g. grid), whereas unbound materials are distributed three dimensionally. Accordingly, said surface enables both a two and a three dimensional separation.Type: ApplicationFiled: November 11, 2005Publication date: July 3, 2008Applicant: ORION DIAGNOSTICA OYInventors: Juhani Luotola, Hannu Nikula, Mira Murtovuori
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Publication number: 20080160639Abstract: An embodiment of the invention relates to a device comprising (1) an array of electromagnetic elements comprising coils, metal cores, and metal core heads, and (2) a controller that is adapted to control a current for one or more coils individually, to vary the current for said one or more coils individually, to reverse the current for one or more coils individually, and to generate a specific magnetic flux distribution and gradient across two or more coils; wherein the metal core head is at one end of the coil and the metal core head has a geometry to create a desired magnetic flux, intensity and gradient, in a region of interest between two adjacent coils; further wherein the device is functionally coupled to a fluidic device to concentrate and transport magnetic particles in a fluid without fluidic movement of the fluid.Type: ApplicationFiled: December 29, 2006Publication date: July 3, 2008Inventors: Xina Su, KaiWU Swartz, David J. Schwartz
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Patent number: 7393698Abstract: The present invention relates to magnetic fine particles having immobilized thereto a polymer having an upper critical solution temperature, and a process for producing the same, a method of separating or concentrating a microorganism, a method of purifying, detecting, or concentrating a nucleic acid, a separating agent, a method of separating a biological substance, and a method of converting a substance.Type: GrantFiled: August 20, 2001Date of Patent: July 1, 2008Assignees: National Institute of Advanced Industrial Science and Technology, Chisso CorporationInventors: Hirotaka Furukawa, Noriyuki Ohnishi, Kazunori Kataoka, Katsuhiko Ueno
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Patent number: 7390628Abstract: The present invention concerns novel materials and methods to detect low frequency surface changes utilizing modified microspheres. Upon exposing the surface to the modified microspheres of the subject invention, the microspheres selectively adhere to any targeted surface marker.Type: GrantFiled: December 23, 2004Date of Patent: June 24, 2008Assignee: University of Florida Research Foundation, Inc.Inventor: Christopher D. Batich
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Patent number: 7387901Abstract: Magnetic particles with a metal coat holding target substance captors are made to react with a target substance contained in a specimen in a solution where the magnetic particles are dispersed in a liquid medium. Subsequently, the dispersion of the magnetic particles is applied to a surface having a periodic structure that is adapted to generate plasmon resonance and a change in the plasmon resonance attributable to the concentration of the target substance held on the magnetic particles fixed magnetically to the surface is optically detected to determine the concentration of the target substance in the specimen.Type: GrantFiled: June 24, 2005Date of Patent: June 17, 2008Assignee: Canon Kabushiki KaishaInventors: Satoru Nishiuma, Norihiko Utsunomiya, Takashi Ikeda
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Patent number: 7384559Abstract: A device for treating magnetizable or magnetically attractable particles which are present in a liquid, comprising a holder for one ore more reaction vessel(s) with the liquid containing the particles, at least two movably arranged permanent magnets, a drive unit for moving the permanent magnets; with the permanent magnets being arranged in pairs and opposite each other, and being movable in vertical direction (a, b), and the distance between the two permanent magnets of a pair being dimensioned or adjustable such that a reaction vessel can be positioned therebetween.Type: GrantFiled: November 7, 2002Date of Patent: June 10, 2008Assignee: Chemagen Biopolymer-Technologie AGInventor: Lothar à Brassard
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Patent number: 7384561Abstract: An apparatus for separating a predetermined amount of magnetic particles in a fluid, including a fluid conveyance portion having at least a first flow channel for supplying a fluid containing the magnetic particles, a second flow channel for discharging the separated magnetic particles, and an intersection at which the first flow channel and the second flow channel intersect each other, a capture unit for capturing a predetermined amount of the magnetic particles in the fluid supplied from the first flow channel to the intersection when a magnetic field is applied to the intersection of the fluid conveyance portion, and a discharge unit for removing the magnetic field applied to the intersection to discharge the magnetic particles captured by the capture unit by way of a medium introduced into the second flow channel.Type: GrantFiled: June 24, 2005Date of Patent: June 10, 2008Assignee: Canon Kabushiki KaishaInventor: Norihiko Utsunomiya
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Patent number: 7384798Abstract: A method of detecting analytes in a microfluidic sample includes introducing at least one type of analyte, at least one type of carrier particle, and a fluid into a mixing chamber of a system. The at least one type of analyte binds to a site on the at least one type of carrier particle to form a microfluidic suspension including at least one analyte-bound particle suspended in the fluid. The at least one analyte-bound particle is separated from the fluid by exposing the suspension to physical forces, magnetic forces, or combinations thereof. A spectrophotometric property of a solution is altered using the at least one analyte-bound particle. The altered spectrophotometric property is measured with an optical detection system.Type: GrantFiled: October 31, 2006Date of Patent: June 10, 2008Assignee: Hewlett-Packard Development Company, L.P.Inventors: Philip H. Harding, Christopher C. Beatty, Kenneth J. Ward
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Publication number: 20080131978Abstract: A novel method of detecting and measuring the presence or amount of an analyte in a sample with high sensitivity and good simplicity is provided. A method of detecting an analyte, which comprises binding the analyte to a labeled specific binding substance to form a conjugate and detecting a magnetic signal from the conjugate to detect the analyte, wherein the labeled specific binding material comprises a substance capable of specifically binding to the analyte, a spacer and particular magnetic beads, and wherein the specific binding substance is coupled to the magnetic beads via the spacer.Type: ApplicationFiled: February 2, 2005Publication date: June 5, 2008Applicant: Asahi Kasei Kabushiki KaishaInventors: Mariko Fujimura, Kenji Matsuyama, Katsuya Watanabe
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Patent number: 7371584Abstract: Novel magnetic assay methods and systems. According to a preferred embodiment, a chromatographic medium is provided that is designed to be contacted with a test solution having activated magnetic particles such that the solution flows bilaterally thereacross. A magnetic field, generated by a magnet or electromagnet, is selectively applied to the medium which causes the charged particles to become substantially bound at a site on the medium specified by the position of the magnet. The assay is multi-configurable and can be modified to isolate target cells and grow cell cultures.Type: GrantFiled: December 8, 2006Date of Patent: May 13, 2008Assignee: Wavesence, Inc.Inventor: Christopher Feistel
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Patent number: 7371830Abstract: Magnetic particles are prepared containing a magnetic core coated with a glass layer having a substantially pore-free glass surface or having pores with a diameter of less than 10 nm. The particles are used for separating biological material such as nucleic acids. A preferred process of preparing the particles is by forming a mixture of magnetic cores with a sol formed from an alcohol and a metal alkoxide, spray-drying the mixture to coat the cores with a layer of gelled sol, and heating the coated cores to obtain the magnetic glass particles. Preferably, the particles have an average particle size of less than 100 ?m. The magnetic core may be a composite material containing a mica core and magnetite particles immobilized on the mica core, and the glass layer may contain boron oxide. Magnetic core materials include magnetite (Fe3O4) and Fe2O3.Type: GrantFiled: January 24, 2005Date of Patent: May 13, 2008Assignee: Roche Diagnostics GmbHInventors: Jörg Kleiber, Thomas Walter, Herbert Harttig, Christop Lesniak, Martin Mennig, Michael Riedling, Helmut Schmidt