Glass Or Silica Patents (Class 436/527)
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Patent number: 7190445Abstract: An optical sensor device for determining the presence or concentration of an analyte, contains a waveguide disposed over a light source and a light detector mounted on a surface of a substrate and separated by an internal baffle, wherein the waveguide has a thickness corresponding to a far field emission point of the light source as determined by a light shielding baffle between the light source and light detector. An analyte indicator matrix is disposed on the outer surface of the waveguide. The sensor device geometry takes advantage of only direct illumination of the indicator matrix, and direct collection of indicator matrix illumination, without any significant reflection by said waveguide. Undesirable light noise generated by the light source passes directly out of the device through the waveguide.Type: GrantFiled: June 24, 2005Date of Patent: March 13, 2007Assignee: Sensors for Medicine and Science, Inc.Inventors: Arthur E. Colvin, Jr., Steven J. Walters
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Patent number: 7186566Abstract: Chromatographic strip is used in combination with a transmittance detecting system in a test to quantitate analytes in biological fluid. Chemical reagents or conjugate labels are simply absorbed on the passages' materials of the strip. The substrates, affinity reagents, or antibodies are immobilized on transparent beads in the detection cell of the strip. The biological fluid passes through the strip. The captured analytes are detected by transmittance detection for quantification. Uncaptured elements and interferences in the fluid are drained to the absorbent portion of the strip when the fluid passes the cell as a wash. This chromatographic strip with an analyte capture zone simplifies the procedures that a transmittance detecting system alone cannot overcome. Adjustable light path of the cells in the strip overcome the sensitivity limitation of reflectance detection.Type: GrantFiled: July 28, 2003Date of Patent: March 6, 2007Inventor: Suyue Qian
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Patent number: 7183119Abstract: The present invention relates to a method of detecting biological analytes comprising suspending a target analyte in a suspending solution containing polymeric particles marked with a probe, wherein the probe has an affinity for said target analyte; adding recognition unit-peroxidase conjugate marker to the suspending solution; forming a complex of the target analyte, the polymeric particles marked with a probe, and the recognition unit-peroxidase conjugate marker; contacting a gelatin surface with the suspending solution; adding developer to the suspending solution in contact with the gelatin surface in the presence of phenol to attach the complex to the gelatin surface; washing the gelatin surface; and detecting the complex attached to the gelatin surface.Type: GrantFiled: November 15, 2004Date of Patent: February 27, 2007Assignee: Eastman Kodak CompanyInventors: Tiecheng A. Qiao, Brian J. Kelley
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Patent number: 7179638Abstract: Microarrays are prepared by using a separate fiber for each compound being used in the microarray. The fibers are bundled and sectioned to form a thin microarray that may be glued to a backing.Type: GrantFiled: January 25, 2002Date of Patent: February 20, 2007Assignee: Large Scale Biology CorporationInventors: N. Leigh Anderson, Norman G. Anderson, James A. Braatz
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Patent number: 7172905Abstract: A substrate comprises a surface, and a plurality of moieties, on at least a portion of the surface. The moieties are moieties of formula: Surf-L—Q—T, where —T comprises a reactant ligand, and Surf- designates where the moiety attaches to the surface. The substrate can be made into a protein chip by the reaction of a reactant ligand and a fusion polypeptide, where the fusion polypeptide includes a capture polypeptide moiety which corresponds to the reactant ligand.Type: GrantFiled: August 7, 2001Date of Patent: February 6, 2007Assignee: The University of ChicagoInventors: Milan Mrksich, Christian Hodneland
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Patent number: 7169550Abstract: A biosensor includes a substrate with a receptive material layer of radiation-absorbing member (RAM)-tagged biomolecules disposed thereon. The receptive material is specific for an analyte of interest. A pattern of active and deactivated areas of the receptive material are defined in the receptive material layer by a masking process wherein areas are exposed through a mask with a light source to induce deactivation.Type: GrantFiled: September 26, 2002Date of Patent: January 30, 2007Assignee: Kimberly-Clark Worldwide, Inc.Inventors: Curtis Sayre, Rosann Kaylor, David Cohen
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Patent number: 7166475Abstract: This invention relates to a method for analyzing a sample comprising: immobilising a polypeptide to a physical support; contacting the immobilised polypeptide with a test sample which may contain an agent capable of modifying the immobilised polypeptide; contacting the immobilised polypeptide with a binding partner polypeptide, wherein the binding of this partner polypeptide to the immobilised polypeptide is at least partly dependent on the modification state of the immobilised polypeptide; and measuring the association of the binding partner polypeptide to the immobilised polypeptide.Type: GrantFiled: February 26, 1999Date of Patent: January 23, 2007Assignee: Cyclacel Ltd.Inventors: John Colyer, Roger K. Craig, Antonio Maschio, Mokdad Mezna
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Patent number: 7160735Abstract: Compositions and methods are disclosed for detecting multiple target analytes in a sample using microparticles having molecular tags attached by cleavable linkages. Generally, an assay mixture is formed comprising a sample and a reagent comprising multiple such microparticles under conditions that permit stable complexes to form between binding moieties on the surfaces of the microparticles and the analytes. In one aspect of the invention, the a second binding composition is added so that complexes form among the microparticle-bound binding moieties, the analytes, and second binding moieties of the second binding composition. Such second binding moieties have cleavage-inducing moieties attached that upon activation cause the cleavage of the cleavable linkages and the release of molecular tags. Released molecular tags are separated and the presence and/or amount of the target analytes are determined based on the analysis of the released and separated molecular tags.Type: GrantFiled: November 8, 2002Date of Patent: January 9, 2007Assignee: Monogram Biosciences, Inc.Inventors: Peter Dehlinger, Sharat Singh, Hrair Kirakossian
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Label-free methods for performing assays using a colorimetric resonant reflectance optical biosensor
Patent number: 7153702Abstract: Methods are provided for detecting biomolecular interactions. The use of labels is not required and the methods can be performed in a high-throughput manner. The invention also relates to optical devices.Type: GrantFiled: September 9, 2002Date of Patent: December 26, 2006Assignee: SRU Biosystems, Inc.Inventors: Bo Lin, Jane Pepper, Brian T. Cunningham, John Gerstenmaier, Peter Li, Jean Qiu, Homer Pien -
Patent number: 7153682Abstract: Provided are peptidomimetic protein-binding arrays, their manufacture, use, and application. The protein-binding array elements of the invention include a peptidomimetic segment linked to a solid support via a stable anchor. The invention contemplates peptidomimetic array element library synthesis, distribution, and spotting of array elements onto solid planar substrates, labeling of complex protein mixtures, and the analysis of differential protein binding to the array. The invention also enables the enrichment or purification, and subsequent sequencing or structural analysis of proteins that are identified as differential by the array screen. Kits including proteomic microarrays in accordance with the present invention are also provided.Type: GrantFiled: July 3, 2002Date of Patent: December 26, 2006Assignee: Chiron CorporationInventors: Deborah Charych, Eric Beausoleil, Ronald N. Zuckermann
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Patent number: 7150856Abstract: An on-spot selectively activated hydrophobic slide/microarray. The preparation method relates to a hydrophobic copolymer prepared by blending, grafting or co-polymerization of a hydrophobic material and a compound bearing a functional group protected by a protecting group, wherein the functional group is imide or cyclic amide, and the protecting group is a photo acid group such as a tosyloxy group. The hydrophobic copolymer coated on a substrate is then subjected to selective photolithographical activation so that the slide will have functional active copolymer spots separated by inactive copolymers. The resulting slide is suitable for the preparation of high-density and high-efficiency bio-chip/microarray.Type: GrantFiled: October 5, 2005Date of Patent: December 19, 2006Assignee: Industrial Technology Research InstituteInventors: Bor-Iuan Jan, Jia-Huey Tsao, Chih-Wei Ho, Chao-Chi Pan
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Patent number: 7148056Abstract: Diagnostic devices are disclosed which contain porous material. The porous material preferably comprises silica and alumina. Chemical and/or biological molecules can be bound to the porous material in high concentrations while both having high accessibility to molecules in solution, and retaining their natural conformation. Such diagnostic devices may be used for a wide array of assays including protein (e.g. ELISA) and nucleic acid (e.g. hybridization on chips or beads) detection and/or quantification methods.Type: GrantFiled: March 24, 2001Date of Patent: December 12, 2006Inventor: Mark B. Lyles
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Patent number: 7148058Abstract: Provided are protein microarrays, their manufacture, use, and application. Protein microarrays in accordance with the present invention are useful in a variety preoteomic analyses. Various protein arrays in accordance with the present invention may immobilize large arrays of proteins that may be useful for studying protein-protein interactions to improve understanding of disease processes, facilitating drug discovery, or for identifying potential antigens for vaccine development. The protein array elements of the invention are native or modified proteins (e.g., antibodies or fusion proteins). The protein array elements may be attached directly to a organic functionalized mirrored substrate by a binding reaction between functional groups on the substrate (e.g., amine) and protein (e.g., activated carboxylic acid). Techniques for chemical blocking of the arrays are also provided.Type: GrantFiled: July 3, 2002Date of Patent: December 12, 2006Assignee: Chiron CorporationInventors: Deborah Charych, Ronald N. Zuckermann
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Patent number: 7144700Abstract: Methods, employing a polycyclic hydrocarbon or a polycyclic heteroaromatic compound as sensitizers, are provided to increase the efficiency of removing, by irradiation, photolabile protecting groups that mask reactive sites on synthesis intermediaries. Preferred groups of photolabile protecting moieties include: ((?-methyl-2-nitropiperonyl)-oxy)carbonyl (MeNPOC), ((Phenacyl)-oxy)carbonyl (PAOC), O-(9-phenylxanthen-9-yl) (PIXYL), and ((2-methylene-9,10-anthraquinone)-oxy)carbonyl (MAQOC). In conjunction with using the sensitizers and protecting groups described above, a method of forming, from component molecules, a plurality of compounds on a support, each compound occupying a separate predefined region of the support is provided. These resulting solid-phase arrays are useful, for example, to assay for the presence of biochemical products in biological samples.Type: GrantFiled: July 21, 2000Date of Patent: December 5, 2006Assignee: Affymetrix, Inc.Inventors: Glenn McGall, Daniel E. Falvey, Jacqueline A. Fidanza, Brian M. Feldman
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Patent number: 7138268Abstract: An assay plate for detecting the presence of a mobile reactant that binds to a immobilized reactant and the methods of making and using the same. An assay plate according to the present invention includes a substrate and at least one dried aliquot of the immobilized reactant, the immobilized reactant being bound to the surface of the substrate. The immobilized reactant binds the mobile reactant when a solution containing the mobile reactant is brought into contact with the immobilized reactant. The mobile and immobilized reactants may be any pair of biological compounds that have a specific affinity for one another. For example the reactants may be nucleic acids or antibody-antigen pairs. The preferred embodiment of an assay plate according to the present invention includes a plurality of assay spots, each spot having a different immobilized reactant or concentration thereof.Type: GrantFiled: June 29, 2001Date of Patent: November 21, 2006Assignee: Agilent Technologies, Inc.Inventor: Kay Lichtenwalter
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Patent number: 7135343Abstract: The present invention relates to bio-molecule resistant surfaces for use in assays, particularly in assay devices such as arrays and microfluidic devices. The biomolecule resistant surface of the present invention are prepared by coating a substrate with hydrophilic terminated alkoxysilanes having formula (A): wherein R is an alkyl group of a size that allows for sufficient hydrolysis and n is 1, 2 or 3; R1 is a hydrophilic moiety; LC is a C1 to a C10 linker chain consisting of a group selected from alkyl, aryl, alkaryl and aralkyl and m is 1, 2, or 3; R2 is a C1 to a C7 alkyl group and x is 0, 1, or 2; and m+n+x is equal to 4.Type: GrantFiled: June 17, 2002Date of Patent: November 14, 2006Assignee: Agilent Technologies, Inc.Inventors: Dan-Hui Yang, Namyong Kim, Janelle Gunther
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Patent number: 7125674Abstract: An improved apparatus for conducting a multiplicity of individual and simultaneous binding reactions is described. The apparatus comprises a substrate on which are located discrete and isolated sites for binding reactions. The apparatus is characterized by discrete and isolated regions that extend through a substrate and terminate on a second surface thereof, such that when a test sample is applied to the substrate, it is capable of penetrating through each such region during the course of the binding reaction. The apparatus is especially useful for sequencing by hybridization of DNA molecules.Type: GrantFiled: December 27, 2004Date of Patent: October 24, 2006Assignee: UT-Battelle, LLCInventor: Kenneth L. Beattie
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Patent number: 7122381Abstract: A selective adsorption material made by the process comprising: a) non-cavalently or reversibly-covalently binding a print molecule to at least two styrene, acrylate or silica monomers, each of which monomers brinds to said print molecule by means of a different functional group; b) immobilizing said bound monomers by being polymerized to each other in the presence of an effective amount of cross-linker; and c) removing said print molecule from said polymer by extraction to leave a cavity in said polymer which is stereo-tailored to a biological molecule of interest; and wherein said print molecule is the biological molecule of interest.Type: GrantFiled: September 4, 1992Date of Patent: October 17, 2006Inventors: Magnus Glad, Maria Kempe, Klaus Mosbach
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Patent number: 7122384Abstract: Microparticle-based analytical methods, systems and applications are provided. Specifically, the use of resonant resonant light scattering as an analytical method for determining either or both a particle's identity and the presence and optionally, the concentration of one or more particular target analytes is described. Applications of these microparticle-based methods in biological and chemical assays are also disclosed.Type: GrantFiled: November 5, 2003Date of Patent: October 17, 2006Assignee: E. I. du Pont de Nemours and CompanyInventors: James M. Prober, Xiumin Cui, Rudy J. Dam, Edwin R. Hendrickson, Xueping Jiang, Michael P. Perry, Larry Eugene Steenhoek
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Patent number: 7118922Abstract: The present invention allows for regeneration of any chemical and biological sensor systems without totally destroying the primary antibodies in the system. The present invention provides regeneration of the sensor by debinding of the antigen/antibody complex utilizing a controlled electrical impulse. The technique is generic and can be used with most immunoassay-based detectors.Type: GrantFiled: August 16, 2004Date of Patent: October 10, 2006Assignee: University of South FloridaInventors: Shekhar Bhansali, Beverly A. Rzigalinski, Hyoungjin Cho
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Patent number: 7118908Abstract: An on-spot selectively activated hydrophobic slide/microarray. The preparation method relates to a hydrophobic copolymer prepared by blending, grafting or co-polymerization of a hydrophobic material and a compound bearing a functional group protected by a protecting group, wherein the functional group is imide or cyclic amide, and the protecting group is a photo acid group such as a tosyloxy group. The hydrophobic copolymer coated on a substrate is then subjected to selective photolithographical activation so that the slide will have functional active copolymer spots separated by inactive copolymers. The resulting slide is suitable for the preparation of high-density and high-efficiency bio-chip/microarray.Type: GrantFiled: July 8, 2002Date of Patent: October 10, 2006Inventors: Bor-Iuan Jan, Jia-Huey Tsao, Chih-Wei Ho, Chao-Chi Pan
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Patent number: 7112453Abstract: This invention provides methods of retentate chromatography for resolving analytes in a sample. The methods involve adsorbing the analytes to a substrate under a plurality of different selectivity conditions, and detecting the analytes retained on the substrate by desorption spectrometry. The methods are useful in biology and medicine, including clinical diagnostics and drug discovery.Type: GrantFiled: August 5, 2002Date of Patent: September 26, 2006Assignee: Ciphergen Biosystems, Inc.Inventors: T. William Hutchens, Tai-Tung Yip
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Patent number: 7112452Abstract: A method and sensor are provided for detecting the binding of a probe and a target biomolecule by measuring a difference in the shear stress on the surface of the sensor before and after hybridization of the target molecule to the probe, such as nucleic acids or proteins. The shear stress may be measured sensitively and conveniently as an electrical signal without additional fluorescent labeling and without use of expensive additional devices.Type: GrantFiled: October 23, 2002Date of Patent: September 26, 2006Assignee: Samsung Electronics Co., Ltd.Inventors: Yoon-kyoung Cho, Sun-hee Kim, Kwang-wook Oh, Geun-bae Lim, Dae-sung Yoon
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Patent number: 7109024Abstract: This invention features a biomolecule-bound substrate that includes a support made of an organic polymer and having an unmodified surface; and a plurality of unmodified biomolecules immobilized on the unmodified surface. The organic polymer is acrylic resin, polypropylene, polystyrene, polyethylene, polyvinyl chloride, polysulfone, polycarbonate, cellulose acetate, rubber, latex, polyethylene terephthalate, acrylonitrile butadiene styrene, acrylonitrile styrene, or a combination thereof. The substrate is formed by placing the unmodified biomolecules on the unmodified surface followed by ultraviolet irradiation.Type: GrantFiled: April 7, 2003Date of Patent: September 19, 2006Assignee: Dr. Chip Biotechnology Inc.Inventors: Kan-Hung Lee, Yu-Hau Shih, Chuan-Mei Tsai, Yih-Weng Wang, Hsiung Hsiao, Chi-Horng Bair, Shin-Hwan Wang
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Patent number: 7105340Abstract: An on-spot selectively activated hydrophobic slide/microarray. The preparation method relates to a hydrophobic copolymer prepared by blending, grafting or co-polymerization of a hydrophobic material and a compound bearing a functional group protected by a protecting group, wherein the functional group is imide or cyclic amide, and the protecting group is a photo acid group such as a tosyloxy group. The hydrophobic copolymer coated on a substrate is then subjected to selective photolithographical activation so that the slide will have functional active copolymer spots separated by inactive copolymers. The resulting slide is suitable for the preparation of high-density and high-efficiency bio-chip/microarray.Type: GrantFiled: October 5, 2005Date of Patent: September 12, 2006Inventors: Bor-Iuan Jan, Jia-Huey Tsao, Chih-Wei Ho, Chao-Chi Pan
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Patent number: 7101682Abstract: Methods, compositions and kits are disclosed. The methods are directed to determining the presence of one or more analytes in a sample suspected of containing any one of a plurality of the analytes. A combination is provided comprising in a medium (i) the sample, (ii) a binding partner for each of the analytes, (iii) for each of the analytes, a first reagent comprising a member of a signal producing system, a ligand and an analyte analog, and (iv) a second reagent comprising a binding partner for the ligand. The binding of the binding partner for the ligand is affected by the presence of an analyte and alters the amount of signal produced by the member of the signal producing system. The signal thus is modulated if one or more of the analytes are present in the sample. The amount of the signal is determined and is related to the presence of one or more of the analytes in the sample. The method may be homogeneous or heterogeneous.Type: GrantFiled: November 7, 2005Date of Patent: September 5, 2006Assignee: Dade Behring Marburg GmbHInventors: Edwin F. Ullman, Marcel Pirio, Mary C. Ericson, Daniel B. Wagner, Dariush Davalian
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Patent number: 7101718Abstract: A semiconductor nanocrystal compound is described capable of linking to an affinity molecule. The compound comprises (1) a semiconductor nanocrystal capable of emitting electromagnetic radiation and/or absorbing energy, and/or scattering or diffracting electromagnetic radiation—when excited by an electromagnetic radiation source or a particle beam; and (2) at least one linking agent, having a first portion linked to the semiconductor nanocrystal and a second portion capable of linking to an affinity molecule. The compound is linked to an affinity molecule to form a semiconductor nanocrystal probe capable of bonding with a detectable substance. subsequent exposure to excitation energy will excite the semiconductor nanocrystal in the probe causing the emission of electromagnetic radiation.Type: GrantFiled: August 12, 2004Date of Patent: September 5, 2006Assignee: The Regents of the University of CaliforniaInventors: Shimon Weiss, Marcel Bruchez, Jr., Paul Alivisatos
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Patent number: 7097974Abstract: A method for producing a support for determining analytes. The method comprises the steps of (a) providing a support comprising at least one channel, comprising a conduit having an intake and an outlet for passing fluid from the intake to the outlet, in the support body, (b) passing liquid with building blocks for synthesizing polymeric receptors through the channel or channels of the support body, (c) site- and/or time-specifically immobilizing the receptor building blocks in each case on predetermined positions in the channel or channels by illumination and (d) repeating steps (b) and (c) until the required receptors have been synthesized in each case on the predetermined positions.Type: GrantFiled: August 27, 1999Date of Patent: August 29, 2006Assignee: febit biotech GmbHInventors: Cord F. Stähler, Peer F. Stähler, Manfred Müller, Fritz Stähler, Hans Lindner
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Patent number: 7098041Abstract: An analyzer useful in determining the presence of an analyte using a diffraction based sensing device and methods and systems using this device. The present invention may be used with a variety of different diffraction-based diagnostic methods and systems. The analyzer enhances the accuracy and/or usefulness of these devices in detecting analytes, while providing more ease-of-use and convenience to the user. The analyzer may include a light source, a photodiode, a microprocessor and a display system for informing the user of the result. Other features include mirrors, lenses, a sample holder, and a mask for blocking out some light. The analyzer and related method and system may be used in a large number of environments, including commercial, professional, and individual.Type: GrantFiled: December 11, 2001Date of Patent: August 29, 2006Assignee: Kimberly-Clark Worldwide, Inc.Inventors: Rosann Marie Kaylor, Difei Yang, Zdravko Savov Atanassov, Michael Eugene Knotts
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Patent number: 7094595Abstract: Methods and compositions are provided for detecting biomolecular interactions. The use of labels is not required and the methods can be performed in a high-throughput manner. The invention also provides optical devices useful as narrow band filters.Type: GrantFiled: August 15, 2001Date of Patent: August 22, 2006Assignee: SRU Biosystems, Inc.Inventors: Brian Cunningham, Jane Pepper, Bo Lin, Peter Li, Homer Pien
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Patent number: 7090988Abstract: A device for detecting the presence of an antigen including (1) a cell having antibodies which are expressed on the surface of the cell and are specific for the antigen to be detected, where binding of the antigen to the antibodies results in an increase in calcium concentration in the cytosol of the cell, the cell further having a emitter molecule which, in response to the increased calcium concentration in the cytosol, emits a photon; (2) a liquid medium for receiving the antigen and in which the cell is immersed; and (3) an optical detector arranged for receiving the photon emitted from the cell.Type: GrantFiled: August 3, 2004Date of Patent: August 15, 2006Assignee: Massachusetts Institute of TechnologyInventors: Todd H. Rider, Laura Bortolin
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Patent number: 7091049Abstract: An enhanced diffraction based biosensor system and method are provided for detecting an analyte of interest in a test medium. The system incorporates at least one additional detection tag substance with the analyte of interest, the tag emitting a measurable parameter that is different from optical diffraction characteristics of the analyte. The biosensor may be a “fluoroptical” system wherein the detection tag is a fluorescence emitting substance, including fluorescent-labeled diffraction enhancing elements. The enhanced diffraction biosensor system may determine the presence of analytes in biological fluids both qualitatively and quantitatively.Type: GrantFiled: June 26, 2002Date of Patent: August 15, 2006Assignee: Kimberly-Clark Worldwide, Inc.Inventors: Rameshbabu Boga, Chibueze Obinna Chidebelu-Eze, Rosann M. Kaylor, Xuedong Song
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Patent number: 7090993Abstract: This invention relates to a method of assaying pyrrole-containing biological compounds and chemical compositions that can be used in the method. The method involves contacting a biological compound with one of: a) a bound or bindable derivatizing agent which forms a reaction product with the biological compound, followed by exposure to a detectable molecule which forms a complex with the reaction product; or b) a derivatizing agent which forms a reaction product with the biological compound, followed by exposure to a bound binding agent specific to the biological compound in the reaction product; or c) a binding agent specific to the biological compound, followed by exposure to a derivatizing agent which forms a reaction product with the biological compound, and determining the amount of bound biological compound. There is also provided a method of preparing an antigen.Type: GrantFiled: September 26, 2003Date of Patent: August 15, 2006Assignee: Rowett Research InstituteInventors: Jeffrey D. Brady, Simon P. Robins
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Patent number: 7074610Abstract: An efficient method for the microfabrication of electronic devices which have been adapted for the analyses of biologically significant analyte species is described. The techniques of the present invention allow for close control over the dimensional features of the various components and layers established on a suitable substrate. Such control extends to those parts of the devices which incorporate the biological components which enable these devices to function as biological sensors. The materials and methods disclosed herein thus provide an effective means for the mass production of uniform wholly microfabricated biosensors. Various embodiments of the devices themselves are described herein which are especially suited for real time analyses of biological samples in a clinical setting. In particular, the present invention describes assays which can be performed using certain ligand/ligand receptor-based biosensor embodiments.Type: GrantFiled: August 30, 2001Date of Patent: July 11, 2006Assignee: i-Stat CorporationInventors: Stephen N. Cozzette, Graham Davis, Jeanne A. Itak, Imants R. Lauks, Randall M. Mier, Sylvia Piznik, Nicolaas Smit, Susan J. Steiner, Paul Van Der Werf, Henry J. Wieck
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Patent number: 7074622Abstract: The present invention relates to a method of particle separation comprising preparing a suspension of particles containing at least one recognizable target particle in a suspending solution, labeling the target particle with a conjugate marker, wherein the conjugate marker comprises at least one recognition unit for the recognizable target particle and at least one peroxidase enzyme, contacting a gelatin surface with the suspending solution, adding developer to the suspending solution in contact with the gelatin surface and in the presence of phenol to attach the target particle to the gelatin surface, and washing the gelatin surface to remove unattached particles. The method may also include detecting the presence of the target particle on the gelatin surface as well as detaching and recovering the attached particles after removal of the non-target particles.Type: GrantFiled: November 15, 2004Date of Patent: July 11, 2006Assignee: Eastman Kodak CompanyInventors: Tiecheng A. Qiao, Yun C. Chang, Eric R. Schmittou, Thomas I. Penner
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Patent number: 7070941Abstract: The invention provides methods and compositions for azide tagging of biomolecules. In one embodiment of the invention, proteins are tagged by metabolic incorporation of prenylated azido-analog substrates. Examples of such analogs are azido farnesyl diphosphate and azido farnesyl alcohol. The azido moiety in the resulting modified proteins provides an affinity tag, which can be chemoselectively captured by an azide-specific conjugation reaction, such as the Staudinger reaction, using a phosphine capture reagent. When the capture agent is biotinylated, the resulting conjugates can be detected and affinity-purified by streptavidin-linked- HRP and streptavidin-conjugated agarose beads, respectively. The invention allows detection and isolation of proteins with high yield, high specificity, and low contamination without harsh treatment of proteins.Type: GrantFiled: November 17, 2003Date of Patent: July 4, 2006Assignee: Board of Regents, The University of Texas SystemInventors: Yingming Zhao, John R. Falck
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Patent number: 7070987Abstract: Methods and compositions are provided for detecting biomolecular interactions. The use of labels is not required and the methods can be performed in a high-throughput manner. The invention also provides optical devices useful as narrow band filters.Type: GrantFiled: January 28, 2002Date of Patent: July 4, 2006Assignee: SRU Biosystems, Inc.Inventors: Brian T. Cunningham, Jane Pepper, Bo Lin, Peter Li, Jean Qiu, Homer Pien
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Patent number: 7067267Abstract: This invention relates to a method of assaying pyrrole-containing biological compounds and chemical compositions that can be used in the method. The method involves contacting a biological compound with one of: a) a bound or bindable derivatizing agent which forms a reaction product with the biological compound, followed by exposure to a detectable molecule which forms a complex with the reaction product; or b) a derivatizing agent which forms a reaction product with the biological compound, followed by exposure to a bound binding agent specific to the biological compound in the reaction product; or c) a binding agent specific to the biological compound, followed by exposure to a derivatizing agent which forms a reaction product with the biological compound, and determining the amount of bound biological compound. There is also provided a method of preparing an antigen.Type: GrantFiled: September 26, 2003Date of Patent: June 27, 2006Assignee: Rowett Research InstituteInventors: Jeffrey D. Brady, Simon P. Robins
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Patent number: 7060487Abstract: Coupled fiber-optic, evanescent-wave biosensors are improved through the use of configurations which adjust certain optical characteristics for enhanced sensitivity. In the preferred embodiment, this is carried out by inputting light into the coupler at either a different wavelength or multiple wavelengths simultaneously. In alternative embodiments, different modulation schemes and/or interferometric schemes are utilized. For example, at each of the inputs, different carrier frequencies may be used and modulated at lower frequencies, including prime-number frequencies. As the refractive index is changed in the vicinity of the coupling, a shift in the wavelength will induce a phase shift in the baseline signal such that, during data collection, the sensor is able to detect more refined changes. In general, through appropriate choice of input wavelength, fewer operational points will fall in an inefficient local maximum or minimum, thereby affording much greater sensitivity.Type: GrantFiled: March 7, 2002Date of Patent: June 13, 2006Assignee: Veridian Systems DivisionInventor: Michael D. Lackhart
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Patent number: 7052915Abstract: A method for selective labeling of phosphate groups in natural and synthetic oligomers and polymers in the presence of chemically related groups such as carboxylic acid groups. The method is specifically applicable to biological oligomers and polymers, including phosphopeptides, phosphoproteins and phospholipids. In a specific embodiment, selective labeling of phosphate groups in proteins and peptides, for example, facilitates separation, isolation and detection of phosphoproteins and phosphopeptides in complex mixtures of proteins. Selective labeling can be employed to selectively introduce phosphate labels at phosphate groups in an oligomer or polymer, e.g., in a peptide or protein. Detection of the presence of the label, is used to detect the presence of the phosphate group in the oligomer or polymer. The method is useful for the detection of phosphoproteins or phosphopeptides.Type: GrantFiled: June 12, 2001Date of Patent: May 30, 2006Assignee: University of WashingtonInventors: Ruedi Aebersold, Huilin Zhou
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Patent number: 7052650Abstract: Apparatus for the manipulating and testing of molecules and in particular of DNA comprising a surface on which the molecule is anchored on multiple points at one end and a paramagnetic bead on which said molecule is anchored on multiple points at its other end, magnetic means for applying a force to the bead, said magnetic means being used to control the stretching and rotation of said bead and molecule, optical magnification means and a camera for the visualisation of said bead, computer means to which the images of the camera are transmitted, said computer means comprising means for analyzing the motions of the bead. A method for the manipulating and testing of molecules and in particular of DNA in which a molecule is anchored at one end to a fixed surface and at its other end to a paramagnetic bead wherein said molecule is anchored on multiple points at each of said ends.Type: GrantFiled: June 6, 2002Date of Patent: May 30, 2006Assignee: Center National de la Recherche Scientifique (CNRS)Inventors: Terrence R. Strick, Jean F. Allemand, David Bensimon, Aaron Bensimon, Vincent Croquette
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Patent number: 7049064Abstract: The present invention is related to a method for making microarrays comprising the steps of: submitting the surface of a solid support to an oxidation of chemical groups present on said surface in order to allow the formation of aldehyde functions upon the surface of said solid support, covalently binding upon said aldehyde functions capture molecules designed for the detection, the identification, the quantification and/or the recovery of complementary target biological or chemical molecules of interest; said covalent binding resulting in an array comprising a density of at least 4 or more discrete regions/cm2 of solid support surface, each of said discrete surface regions being bound with a species of capture molecules.Type: GrantFiled: April 10, 2001Date of Patent: May 23, 2006Assignee: Eppendorf Array Technologies SA (EAT)Inventors: Laszlo Hevesi, Laurent Jeanmart, José Remacle
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Patent number: 7045365Abstract: A method for producing a derivatized aldehydic support matrix material includes activating surface hydroxyl groups on the support matrix material and reacting the activated hydroxyl groups with an aldehydic alkoxy silane. The derivatized aldehydic support matrix material produced is useful for immobilizing bio-molecules in biological applications.Type: GrantFiled: May 15, 2003Date of Patent: May 16, 2006Assignee: United Chemical Technologies Inc.Inventors: Ann N. Coyne, John H. MacMillan, Michael J. Telepchak
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Patent number: 7041509Abstract: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays. In particular, the devices and methods of the invention are useful in screening large numbers of different compounds for their effects on a variety of chemical, and preferably, biochemical systems.Type: GrantFiled: April 2, 2002Date of Patent: May 9, 2006Assignee: Caliper Life Sciences, Inc.Inventors: J. Wallace Parce, Anne R. Kopf-Sill, Luc J. Bousse
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Patent number: 7037727Abstract: An apparatus for measuring a medical substance where a prism having a high refractive index, a thin metal film formed on one of the surfaces of the prism, a light source for making a light incident upon the thin metal film, and a detector for detecting an incident angle of a light being made incident upon a film on which a plasmon resonance phenomenon is generated on the thin metal film are provided. The medical substance, i.e. antigen as an object to be measured, is fixed to the surface of the thin metal film, and the condition for generating the plasmon resonance phenomenon is varied when a mixture of antibody which is coupled with the medical substance in a specific manner and a sample is made contact with a surface of the thin metal film; and the amount of the medical substance can be detected by recognizing the change with a high sensitivity.Type: GrantFiled: December 4, 1997Date of Patent: May 2, 2006Assignee: DKK CorporationInventors: Norio Miura, Noboru Yamazoe, Taizo Uda
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Patent number: 7033775Abstract: Methods, compositions and kits are disclosed. The methods are directed to determining the presence of one or more analytes in a sample suspected of containing any one of a plurality of the analytes. A combination is provided comprising in a medium (i) the sample, (ii) a binding partner for each of the analytes, (iii) for each of the analytes, a first reagent comprising a member of a signal producing system, a ligand and an analyte analog, and (iv) a second reagent comprising a binding partner for the ligand. The binding of the binding partner for the ligand is affected by the presence of an analyte and alters the amount of signal produced by the member of the signal producing system. The signal thus is modulated if one or more of the analytes are present in the sample. The amount of the signal is determined and is related to the presence of one or more of the analytes in the sample. The method may be homogeneous or heterogeneous.Type: GrantFiled: July 9, 2004Date of Patent: April 25, 2006Assignee: Dade Behring Marburg GmbHInventors: Edwin F. Ullman, Marcel Pirio, Mary C. Ericson, Daniel B. Wagner, Dariush Davalian
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Patent number: 7022515Abstract: Methods and apparatus for evanescent light fluoroimmunoassays are disclosed. The apparatus employs a planar waveguide and optionally has multi-well features and improved evanescent field intensity. The preferred biosensor and assay method have the capture molecules immobilized to the waveguide surface by site-specific coupling chemistry. Additionally, the coatings used to immobilize the capture molecules provide reduced non-specific protein adsorption.Type: GrantFiled: October 5, 2001Date of Patent: April 4, 2006Assignee: University of Utah Research FoundationInventors: James N. Herron, Douglas A. Christensen, Karin D. Caldwell, Vera Janatová, Shao-Chie Huang, Hsu-Kun Wang
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Patent number: 7018830Abstract: Disclosed are devices for detecting the presence of a preselected analyte in a fluid sample. The devices comprise a substrate microfabricated to define a sample inlet port, and a mesoscale flow system that includes a sample flow channel extending from the inlet port. The mesoscale flow system further includes an analyte detection region in fluid communication with the flow channel comprised of a binding moiety for specifically binding the analyte. The detection region is constructed with a mesoscale dimension sufficiently small to enhance binding of the binding moiety and the analyte. The binding moiety may be immobilized in the detection region. The mesoscale detection systems of the invention may be used in a wide range of applications, including the detection of cells or macromolecules, or for monitoring reactions or cell culture growth.Type: GrantFiled: January 21, 2003Date of Patent: March 28, 2006Assignee: The Trustees of the University of PennsylvaniaInventors: Peter Wilding, Larry J. Kricka, Jay N. Zemel
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Patent number: 7014814Abstract: The present invention relates to a support for substances for detection, an apparatus for processing same, a method of processing same, an apparatus for making same, and a method of making same. The object of the present invention is to provide a reliable and high quality technology that can perform a series of processes, consistently, automatically and easily. A support for substances for detection of the present invention is constructed so as to comprise a flexible base member formed to be slender like a thread, string or tape, a variety of substances for detection having predetermined chemical structures and being fixed side by side along the length of the base member, and a supporting member for supporting the base member in a manner that enables expansion, wherein a fixed location of each substance for detection corresponds with the chemical structure thereof.Type: GrantFiled: March 21, 2001Date of Patent: March 21, 2006Assignee: Bio Strand, Inc.Inventor: Hideji Tajima
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Patent number: 7011948Abstract: Disclosed are methods for analyzing molecular binding events in which the formation of ligand/antiligand complexes can be directly detected in a mixture without requiring separation of the components of the mixture from each other by measuring bulk properties of the mixture (i.e., properties that have contributions from several or all of the components present in the mixture). Using these techniques, it is possible to screen libraries without labeling either the target antiligand or ligand. The invention also provides a method for determining the strength of ligand/antiligand binding by further analysis of the same signals.Type: GrantFiled: July 17, 2002Date of Patent: March 14, 2006Assignee: MDS SciexInventors: Robert G. Chapman, Pejman Ghanouni, Igor Shmulevich, David Balaban, Joseph A. Heanue, Andrew P. May, Nikolai Sharkov