With Formation Of A Segmented Stream Patents (Class 436/53)
  • Patent number: 9255866
    Abstract: Fluidic devices and methods associated with mixing of fluids in fluidic devices are provided. In some embodiments, a method may involve the mixing of two or more fluids in a channel segment of a fluidic device. The fluids may be in the form of, for example, at least first, second and third fluid plugs, composed of first, second, and third fluids, respectively. The second fluid may be immiscible with the first and third fluids. In certain embodiments, the fluid plugs may be flowed in series in the channel segment, e.g., in linear order, causing the first and third fluids to mix without the use of active components such as mixers. The mixing of fluids in a channel segment as described herein may allow for improved performance and simplification in the design and operations of fluidic devices that rely on mixing of fluids.
    Type: Grant
    Filed: July 30, 2014
    Date of Patent: February 9, 2016
    Assignee: OPKO Diagnostics, LLC
    Inventors: Matthew Dirckx, Vincent Linder, Jason Taylor
  • Patent number: 9247902
    Abstract: An apparatus and method that allow multiple samples of a liquid in a primary container (here called a ‘pouch’) to be taken with minimal danger of contaminating the liquid in the container during the sampling process are disclosed. In an embodiment, a closed sterile sight chamber is connected with the pouch in a sterile manner to allow fluid to flow into the chamber white at the same time allowing air within the chamber to be displaced through a bacterial fitter to atmosphere. The connection between the sight chamber and the pouch is then terminated (severed or closed), isolating the pouch from the sampling apparatus. Only then are samples of liquid withdrawn from the sight chamber, while allowing air to flow into the chamber through the bacterial filter.
    Type: Grant
    Filed: April 4, 2014
    Date of Patent: February 2, 2016
    Assignee: Noble House Group Pty Ltd.
    Inventors: Avtar Singh Kashmirian, Stephanie M. Norrell, William Leonard Mobbs, Craig Douglas Wilson
  • Patent number: 9149571
    Abstract: The invention relates to a peritoneal dialysis sampling system to be used together with a peritoneal dialysis system which is programmed to deliver fluid to a peritoneal cavity of a patient and to drain the fluid from the cavity, said peritoneal dialysis system comprising a supplying line and supplying means for supplying dialysis fluid to the peritoneal cavity, a draining line and draining means for draining the fluid from the cavity, said peritoneal dialysis sampling system comprising an automatic sampling system which is able to automatically sample volumic fractions of the dialysate contained in the peritoneum of the patient at specific time intervals in order to evaluate the peritoneal membrane characteristics and/or improve the peritoneal dialysis for a given patient.
    Type: Grant
    Filed: June 4, 2012
    Date of Patent: October 6, 2015
    Assignee: DEBIOTECH S.A.
    Inventor: Frédéric Neftel
  • Patent number: 9152150
    Abstract: Systems and methods of manipulating discrete volumes of a first fluid in a second fluid are provided. In some embodiments, discrete volumes can be formed in a conduit. In other embodiments, addition fluid can be added to a discrete volume in a first conduit by injecting the addition fluid at a relatively higher pressure. In some embodiments, discrete volumes that normally would not coalesce can be manipulated to be merged together.
    Type: Grant
    Filed: February 22, 2008
    Date of Patent: October 6, 2015
    Assignee: Applied Biosystems, LLC
    Inventors: Linda G. Lee, Mark F. Oldham, Sam L. Woo, David M. Cox, Richard T. Reel, Peter N. Ma, Ben F. Johnson, Dennis Letho
  • Patent number: 9017617
    Abstract: Apparatuses for preparing a sample are disclosed herein. The apparatuses include a chamber, a first valve at least partially disposed in the first chamber, a second valve at least partially disposed in the first chamber, and a pump comprising an actuator and nozzle.
    Type: Grant
    Filed: June 5, 2013
    Date of Patent: April 28, 2015
    Assignee: Luminex Corporation
    Inventors: Jesus Ching, David Hsiang Hu, Steve Jia Chang Yu, Phillip You Fai Lee
  • Patent number: 8999264
    Abstract: A coating formula and method for surface coating non-porous surfaces. Microfluidic devices including said coating achieve desired properties including increased hydrophilicity, improved adhesion, stability and optical clarity.
    Type: Grant
    Filed: September 26, 2011
    Date of Patent: April 7, 2015
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventor: Michael J. Pugia
  • Patent number: 8961898
    Abstract: The present invention provides a bilayer membrane produced using a microchannel capable of easily forming bilayer membranes such as planar lipid bilayer membranes in large quantities, and a production method thereof. A process for producing a bilayer membrane of the present invention comprises forming a state where two liquid phases or liquid and gaseous phases each containing amphipathic molecules are alternately arranged in a microchannel, discharging one of the two liquid phases or the gaseous phase of the liquid and gaseous phases through branch minichannels formed in the wall on one side or in the walls on both sides to contact the remaining liquid phases adjacent to each other, and thereby forming a side-by-side arrangement of bilayer membranes comprising the amphipathic molecules.
    Type: Grant
    Filed: March 28, 2008
    Date of Patent: February 24, 2015
    Assignee: Tokyo Institute of Technology
    Inventors: Takasi Nisisako, Takahiro Baba
  • Patent number: 8889072
    Abstract: Disclosed are computer-implemented methods of sorting particles from a particle stream in a flow cytometer. The methods include: calculating sort decision making parameters using the raw event data values from a flow cytometer and a sort logic; performing sort logic computations using the sort logic definition and the sort decision making parameters to generate sort decisions; converting the sort decisions into sort commands; and sending the one or more sort commands to the flow cytometer. Sort logic computations may include algorithmically using conditional branching logic, and may include sort logic equations having mathematical functions characterizing one or more regions of interest in multidimensional data space. Such mathematical functions may be determined based on one or more parameters provided by a user. Also disclosed are corresponding systems having a flow cytometer and a computer.
    Type: Grant
    Filed: September 13, 2012
    Date of Patent: November 18, 2014
    Assignee: Beckman Coulter, Inc.
    Inventors: Jeffrey W. Degeal, Charles S. Bay, Edward A. Stanton, Paul Barclay Purcell, George C. Malachowski
  • Patent number: 8883513
    Abstract: The present invention relates to droplet-based particle sorting. According to one embodiment, a droplet microactuator is provided and includes: (a) a suspension of particles; and (b) electrodes arranged for conducting droplet operations using droplets comprising particles. A method of transporting a particle is also provided, wherein the method includes providing a droplet comprising the particle and transporting the droplet on a droplet microactuator.
    Type: Grant
    Filed: February 18, 2011
    Date of Patent: November 11, 2014
    Assignee: Advanced Liquid Logic, Inc.
    Inventors: Michael G. Pollack, Vamsee Pamula, Vijay Srinivasan
  • Patent number: 8857462
    Abstract: The invention provides methods for assessing one or more predetermined characteristics or properties of a microfluidic droplet within a microfluidic channel, and regulating one or more fluid flow rates within that channel to selectively alter the predetermined microdroplet characteristic or property using a feedback control.
    Type: Grant
    Filed: September 9, 2013
    Date of Patent: October 14, 2014
    Assignee: Raindance Technologies, Inc.
    Inventors: Benjamin Miller, Brian Hutchison, Andrew Wilson, Jonathan Larson, Qun Zhong, Yevgeny Yurkovetsky, Darren Link, Mark Weary
  • Patent number: 8845981
    Abstract: A device for volumetric metering a liquid biologic sample is provided. The device includes an initial chamber, a second chamber, a third chamber, a first valve, a second valve and a third valve. The chambers are each configured so that liquid sample disposed in the respective chamber is subject to capillary forces. Each chamber has a volume, and the volume of the initial chamber is greater than the volume of either the second or the third chambers. The valves each have a burst pressure. The burst pressure of the first valve is greater than the third burst pressure. The first valve is in fluid communication with the second chamber. The second valve is disposed between, and is in fluid communication with, the initial chamber and the third chamber. The third valve is in fluid communication with the third chamber.
    Type: Grant
    Filed: December 28, 2012
    Date of Patent: September 30, 2014
    Assignee: Abbott Point of Care, Inc.
    Inventor: Manav Mehta
  • Patent number: 8809070
    Abstract: A dispensing method which dispenses a first liquid contained in a first vessel which stores the first liquid and a second liquid to introduce the first liquid into a second vessel by using a tube.
    Type: Grant
    Filed: June 14, 2012
    Date of Patent: August 19, 2014
    Assignee: Seiko Epson Corporation
    Inventors: Yuji Saito, Fumio Takagi
  • Patent number: 8802441
    Abstract: There is provided a method for synthesizing colloidal nanoparticles comprising the step of creating a reactive three-phase foam containing reactants therein for synthesizing the colloidal nanoparticles.
    Type: Grant
    Filed: March 4, 2011
    Date of Patent: August 12, 2014
    Assignee: National University of Singapore
    Inventors: Saif A. Khan, Suhanya Duraiswamy
  • Patent number: 8784749
    Abstract: This invention provides a digital microfluidic manipulation device and a manipulation method thereof. This device comprises a PDMS membrane having a surface comprising a plurality of hydrophobic microstructures; a plurality of air chambers arranged in an array and placed under the PDMS membrane; and a plurality of air channels, each of which connects to a corresponding one of the plurality of air chambers. When a suction force is transmitted via one of the plurality of air channels to the corresponding air chamber, a portion of the PDMS membrane above the air chamber deforms toward the air chamber, so that the surface morphology and the contact angle of the liquid/solid interface of the surface comprising the plurality of hydrophobic microstructures are altered and thereby to drive droplets.
    Type: Grant
    Filed: December 21, 2012
    Date of Patent: July 22, 2014
    Assignee: National Taiwan University
    Inventors: Jing-Tang Yang, Chao-Jyun Huang, Chih-Yu Hwang
  • Patent number: 8765485
    Abstract: Various aspects of the present invention relates to the control and manipulation of fluidic species, for example, in microfluidic systems. In one aspect, the invention relates to systems and methods for making droplets of fluid surrounded by a liquid, using, for example, electric fields, mechanical alterations, the addition of an intervening fluid, etc. The invention also relates to systems and methods for fusing droplets according to another aspect of the invention, for example, through charge and/or dipole interactions. In some cases, the fusion of the droplets may initiate or determine a reaction. In still another aspect, the invention relates to systems and methods for sorting droplets, e.g., by causing droplets to move to certain regions within a fluidic system. Examples include using electrical interactions (e.g., charges, dipoles, etc.) or mechanical systems (e.g., fluid displacement) to sort the droplets.
    Type: Grant
    Filed: February 23, 2006
    Date of Patent: July 1, 2014
    Assignee: President and Fellows of Harvard College
    Inventors: Darren Roy Link, David A. Weitz, Galder Cristobal-Azkarate, Zhengdong Cheng, Keunho Ahn
  • Patent number: 8735169
    Abstract: The present invention generally relates methods for analyzing agricultural and/or environmental samples using liquid bridges. In certain embodiments, the invention provides a method for analyzing an agricultural sample for a desired trait including obtaining a gene or gene product from an agricultural sample, in which the gene or gene product is in a first fluid; providing a liquid bridge for mixing the gene or gene product with at least one reagent to form a mixed droplet that is wrapped in an immiscible second fluid; and analyzing the mixed droplet to detect a desired trait of the agricultural sample.
    Type: Grant
    Filed: May 20, 2009
    Date of Patent: May 27, 2014
    Assignee: Stokes Bio Limited
    Inventors: Mark Davies, Tara Dalton
  • Patent number: 8722414
    Abstract: The present invention provides a method of controlling a chemical process, the method comprising the steps of: (i) providing a laminar flow of a first fluid, the first fluid providing a first reagent or one or more precursor thereof), a laminar flow of a second fluid, the second fluid providing a second reagent (or one or more precursor thereof) and a laminar flow of barrier fluid; and (ii) causing the first and barrier fluids to contact one another so that the barrier fluid forms a barrier between the first reagent (or one or more precursor thereof) and the second reagent (or one or more precursor thereof) wherein step (ii) comprises forming segments of first fluid encased or sandwiched by barrier fluid, the segments being surrounded by the second fluid, and the barrier fluid is permeable to one or both of the first and second reagents. Devices for performing the method of the present invention are also provided.
    Type: Grant
    Filed: December 20, 2006
    Date of Patent: May 13, 2014
    Assignee: Q Chip Limited
    Inventors: Victoria Louise Workman, Robert Huw Davis, Daniel David Palmer
  • Patent number: 8703491
    Abstract: Disclosed herein is a sample supply device that alternates between the supply of samples from one sample line while cleaning a second sample line and then supplying a second sample from the second sample line while cleaning the first sample line. This is repeated in rapid succession to allow greater speed in analyzing a plurality of samples in a shorter amount of time.
    Type: Grant
    Filed: November 16, 2012
    Date of Patent: April 22, 2014
    Assignee: Vivia Biotech S.L.
    Inventor: Alex Okun
  • Patent number: 8703070
    Abstract: Apparatus for immunoassay includes: a cartridge, including at least one test unit; a pin-film assembly, having a second sealing film, a plurality of pierce mechanisms, and a first actuation unit; a plurality of magnetic particles; at least one first magnetic unit; and at least one second magnetic unit. The test unit includes a plurality of fluid chambers, a plurality of pin chambers, a microchannel structure, a buffer chamber, a detection chamber and a waste chamber. The first actuation unit drives the pierce mechanisms to enable a working fluid to flow into the detection chamber storing the magnetic particles. As the second magnetic unit has a magnetic force larger than that of the first magnetic unit and can move reciprocatingly between a third position and a fourth position, the magnetic particles are driven to move reciprocatingly inside the detection chamber, thereby fully mixing the magnetic particles with the working fluid.
    Type: Grant
    Filed: November 2, 2012
    Date of Patent: April 22, 2014
    Assignee: Industrial Technology Research Institute
    Inventors: Shaw-Hwa Parng, Chih-Wen Yang, Yu-Yin Tsai, Yi-Chau Huang
  • Patent number: 8697008
    Abstract: A method and device for periodically perturbing the flow field within a microfluidic device to provide regular droplet formation at high speed.
    Type: Grant
    Filed: March 9, 2010
    Date of Patent: April 15, 2014
    Assignee: Eastman Kodak Company
    Inventors: Andrew Clarke, Nicholas J. Dartnell, Christopher B. Rider
  • Patent number: 8658367
    Abstract: A microfluidic device is provided for analyzing or sorting biological materials, such as polynucleotides, polypeptides, proteins, enzymes, viruses and cells. The invention can be used for high throughput or combinatorial screening. The device comprises a main channel and an inlet channel that communicate at a droplet extrusion region so that droplets of solution are deposited into an immiscible solvent in the main channel. Droplets can thereafter be sorted according to biological material detected in each droplet.
    Type: Grant
    Filed: May 18, 2012
    Date of Patent: February 25, 2014
    Assignee: California Institute of Technology
    Inventors: Stephen R. Quake, Todd Thorsen
  • Patent number: 8658368
    Abstract: A microfluidic device is provided for analyzing or sorting biological materials, such as polynucleotides, polypeptides, proteins, enzymes, viruses and cells. The invention can be used for high throughput or combinatorial screening. The device comprises a main channel and an inlet channel that communicate at a droplet extrusion region so that droplets of solution are deposited into an immiscible solvent in the main channel. Droplets can thereafter be sorted according to biological material detected in each droplet.
    Type: Grant
    Filed: May 18, 2012
    Date of Patent: February 25, 2014
    Assignee: California Institute of Technology
    Inventors: Stephen R. Quake, Todd Thorsen
  • Patent number: 8651129
    Abstract: An arrangement and method for generating or depositing a stream of m>1 fluid segments, respectively separated by an intermediary medium which does not mix with the fluid segments. The arrangement includes a molded body having a channel for conducting the fluid stream, from which n?m first access lines and n second access lines branch off. The first and second access lines are configured so to be inserted into n wells. The lengths of the first access lines differ from the lengths of the second access lines, and respectively one first valve is arranged in the channel, between the branching location for each second access line and the branching location for each associated first access line, and wherein respectively a second valve is arranged in each second access line.
    Type: Grant
    Filed: October 3, 2011
    Date of Patent: February 18, 2014
    Assignee: Karlsruhe Institut fuer Technologie
    Inventors: Bastian Rapp, André Schumacher
  • Patent number: 8637261
    Abstract: The present invention, provides a flow cytometry apparatus for the detection of particles from a plurality of samples comprising: means for moving a plurality of samples comprising particles from a plurality of respective source wells into a fluid flow stream; means for introducing a separation gas between each of the plurality of samples in the fluid flow stream; and means for selectively analyzing each of the plurality of samples for the particles. The present invention also provides a flow cytometry method employing such an apparatus.
    Type: Grant
    Filed: August 14, 2012
    Date of Patent: January 28, 2014
    Assignee: STC.UNM
    Inventors: Larry A Sklar, Bruce Edwards, Frederick Kuckuck
  • Patent number: 8592215
    Abstract: This invention provides microfabricated devices and methods for detecting, analyzing and sorting biological materials and particles. Droplets containing the particles are provided in an extrusion fluid, passed through a detection region, and then directed into a branch channel according to predetermined characteristics. For example, cells or viral particles contained in droplets of aqueous solvent are flowed past a detector in the nonpolar extrusion fluid decane, and routed into a selected branch channel for subsequent analysis or use.
    Type: Grant
    Filed: September 29, 2011
    Date of Patent: November 26, 2013
    Assignee: California Institute of Technology
    Inventors: Stephen R. Quake, Todd Thorsen
  • Patent number: 8528589
    Abstract: The invention provides methods for assessing one or more predetermined characteristics or properties of a microfluidic droplet within a microfluidic channel, and regulating one or more fluid flow rates within that channel to selectively alter the predetermined microdroplet characteristic or property using a feedback control.
    Type: Grant
    Filed: March 23, 2010
    Date of Patent: September 10, 2013
    Assignee: Raindance Technologies, Inc.
    Inventors: Benjamin Miller, Brian Hutchison, Andrew Wilson, Jonathan Larson, Qun Zhong, Yevgeny Yurkovetsky, Darren Link, Mark Weary
  • Patent number: 8486703
    Abstract: A method of analyzing a chemical composition of a specimen is described. The method can include providing a probe comprising an outer capillary tube and an inner capillary tube disposed co-axially within the outer capillary tube, where the inner and outer capillary tubes define a solvent capillary and a sampling capillary in fluid communication with one another at a distal end of the probe; contacting a target site on a surface of a specimen with a solvent in fluid communication with the probe; maintaining a plug volume proximate a solvent-specimen interface, wherein the plug volume is in fluid communication with the probe; draining plug sampling fluid from the plug volume through the sampling capillary; and analyzing a chemical composition of the plug sampling fluid with an analytical instrument. A system for performing the method is also described.
    Type: Grant
    Filed: September 30, 2010
    Date of Patent: July 16, 2013
    Assignee: UT-Battelle, LLC
    Inventors: Gary J. Van Berkel, Mariam S. Elnaggar
  • Patent number: 8486349
    Abstract: A microfluidic-based flow assay and methods of manufacturing the same are provided. Specifically, the microfluidic flow assay includes a micropatterned surface that induces clot formation and an array of microfluidic channels though which blood flows. The micropatterned surface contains two clotting stimuli, one for inducing platelet adhesion and another for inducing the coagulation cascade.
    Type: Grant
    Filed: March 10, 2011
    Date of Patent: July 16, 2013
    Assignee: Colorado School of Mines
    Inventors: Keith Benjamin Neeves, Ryan R. Hansen
  • Patent number: 8465697
    Abstract: Disclosed are a system and method for regulating flow in an exemplary fluidic device comprising a fluidic stream carrying a transport medium, sample and one or more reagents for analysis and synthesis of reaction products. The flow rate of the fluidic stream is maintained constant by adjusting the flow rate of transport medium to compensate for the introduction of sample and reagents. An embodiment controls the flow rate of transport medium using a pump, a back pressure regulator, and a variable-sized orifice. Single and multiple channel embodiments are disclosed.
    Type: Grant
    Filed: December 31, 2008
    Date of Patent: June 18, 2013
    Assignee: O.I. Corporation
    Inventors: Gary L. Erickson, Craig Ranger
  • Patent number: 8445210
    Abstract: A microfluidic device for analyzing and/or sorting biological materials (e.g., molecules such as polynucleotides and polypeptides, including proteins and enzymes; viruses and cells) and methods for its use are provided. The device and methods of the invention are useful for sorting particles, e.g. virions. The invention is also useful for high throughput screening, e.g. combinatorial screening. The microfluidic device comprises a main channel and an inlet region in communication with the main channel at a droplet extrusion region. Droplets of solution containing the biological material are deposited into the main channel through the droplet extrusion region. A fluid different from and incompatible with the solution containing the biological material flows through the main channel so that the droplets containing the biological material do not diffuse or mix.
    Type: Grant
    Filed: December 15, 2010
    Date of Patent: May 21, 2013
    Assignee: California Institute of Technology
    Inventors: Stephen R. Quake, Todd Thorsen
  • Patent number: 8426213
    Abstract: The present invention relates to providing improved hydrogen peroxide assays, as well as droplet actuators for conducting such assays. The droplet actuators of the invention may be used to conduct droplet-based hydrogen peroxide assays. They may also be associated with detectors for analyzing the results of the hydrogen peroxide assays of the invention. They may be provided as components of systems which control droplet operations and/or detection for conducting the hydrogen peroxide assays. Measurement by the detector may be used to quantify the presence of an analyte in a sample.
    Type: Grant
    Filed: March 5, 2008
    Date of Patent: April 23, 2013
    Assignee: Advanced Liquid Logic Inc
    Inventors: Allen E. Eckhardt, Vijay Srinivasan, Vamsee K. Pamula
  • Patent number: 8420397
    Abstract: Fluid flow devices include a small plate (2), at least one flow channel (20) formed into this small plate, at least one storage channel (221-226) extending from this connection channel, and a set of valves (V1-V6), each of which is suitable for allowing or stopping the flow of fluid in a corresponding storage channel.
    Type: Grant
    Filed: October 12, 2007
    Date of Patent: April 16, 2013
    Assignees: Rhodia Operations, Centre National de la Recherche Scientifique (C.N.R.S.)
    Inventors: Mathieu Joanicot, Philippe Laval, Jean-Baptiste Salmon
  • Patent number: 8372340
    Abstract: Cartridges for the isolation of a biological sample and downstream biological assays on the sample are provided. In one embodiment, a nucleic acid sample is isolated from a biological sample and the nucleic acid sample is amplified, for example by the polymerase chain reaction. The cartridges provided herein can also be used for the isolation of non-nucleic acid samples, for example proteins, and to perform downstream reactions on the proteins, for example, binding assays. Instruments for carrying out the downstream biological assays and for detecting the results of the assays are also provided.
    Type: Grant
    Filed: February 23, 2011
    Date of Patent: February 12, 2013
    Assignee: Luminex Corporation
    Inventors: Dylan Hilmer Bird, Jesus Ching, Bruce A. Johnson, Keith E. Moravick, Bruce Richardson
  • Patent number: 8360244
    Abstract: A new type of asymmetric flow field flow fractionator, A4F, is described permitting improved sample fractionation means by providing a range of available channel lengths within the same A4F unit. With such an apparatus, samples may be optimally separated by performing such fractionations as a function of channel length. The ability to vary channel length within the same A4F unit has heretofore been unavailable.
    Type: Grant
    Filed: June 9, 2008
    Date of Patent: January 29, 2013
    Assignee: Wyatt Technology Corporation
    Inventors: Philip J. Wyatt, Michelle H. Chen, David N. Villalpando
  • Patent number: 8361782
    Abstract: Assays in which samples of biological fluids are dispensed into the inlet port of a microfluidic device are improved in the accuracy and repeatability by dispensing the biological sample and/or associated liquids in small droplets and at timed intervals to control the operation of the microfluidic device.
    Type: Grant
    Filed: March 14, 2008
    Date of Patent: January 29, 2013
    Assignee: Siemens Healthcare Diagnostics, Inc.
    Inventors: Michael J. Pugia, James A. Profitt
  • Patent number: 8343778
    Abstract: The invention encompasses microfluidic microarray assemblies (MMA) and subassemblies and methods for their manufacture and use. In one embodiment, first and second channel plates are provided and are sealingly connected to a test chip in consecutive steps. Each plate includes microfluidic channels configured in a predetermined reagent distribution pattern. The test chip comprises a plurality of discrete test positions, each test position being located at the intersection between a first predetermined reagent pattern and a second predetermined reagent pattern, wherein at least one of said patterns is non-linear. The first channel plate allows the distribution of a first reagent on said test chip, wherein said first reagent is immobilized at said test positions. The second channel plate allows the distribution of a second reagent on said test chip, wherein said second reagent comprises a plurality of different test samples.
    Type: Grant
    Filed: December 12, 2005
    Date of Patent: January 1, 2013
    Assignee: Simon Fraser University
    Inventors: Hua Zhong Yu, Meenakshinathan Parameswaren, Paul Chi Hang Li, Xing Yue Peng, Hong Chen, Wa Lok Chou
  • Patent number: 8333936
    Abstract: The present invention provides for a reagent splitting/dispensing device and method that can prevent contamination of an operation fluid when a reagent dispensing nozzle is in a waiting state and prevent falling of a droplet. The reagent splitting/dispensing method includes disposing and adjusting an air layer between an interface of an operation fluid and a reagent aspirated and subsequently dispensed from a nozzle tip end in an reagent dispensing nozzle.
    Type: Grant
    Filed: January 19, 2011
    Date of Patent: December 18, 2012
    Assignee: Hitachi Plant Technologies, Ltd.
    Inventors: Noe Miyashita, Hideyuki Noda, Masahiro Okanojo
  • Patent number: 8313948
    Abstract: Disclosed herein is a sample supply device that alternates between the supply of samples from one sample line while cleaning a second sample line and then supplying a second sample from the second sample line while cleaning the first sample line. This is repeated in rapid succession to allow greater speed in analyzing a plurality of samples in a shorter amount of time.
    Type: Grant
    Filed: November 5, 2008
    Date of Patent: November 20, 2012
    Assignee: Vivia Biotech S.L.
    Inventor: Alex Okun
  • Patent number: 8298833
    Abstract: A bridge (30) comprises a first inlet port (31) at the end of a capillary, a narrower second inlet port (32) which is an end of a capillary, an outlet port (33) which is an end of a capillary, and a chamber (34) for silicone oil. The oil is density-matched with the reactor droplets such that a neutrally buoyant environment is created within the chamber (34). The oil within the chamber is continuously replenished by the oil separating the reactor droplets. This causes the droplets to assume a stable capillary-suspended spherical form upon entering the chamber (34). The spherical shape grows until large enough to span the gap between the ports, forming an axisymmetric liquid bridge. The introduction of a second droplet from the second inlet port (32) causes the formation of an unstable funicular bridge that quickly ruptures from the, finer, second inlet port (32), and the droplets combine at the liquid bridge (30).
    Type: Grant
    Filed: February 7, 2007
    Date of Patent: October 30, 2012
    Assignee: Stokes Bio Limited
    Inventors: Mark Davies, Tara Dalton, Kieran Curran
  • Patent number: 8273308
    Abstract: This disclosure provides systems, methods, and devices for processing samples on a microfluidic device. One system includes a microfluidic device having an upstream channel, a DNA manipulation zone located downstream from the upstream channel and configured to perform PCR amplification of a sample, a first valve disposed upstream of the DNA manipulation zone, and a second valve disposed downstream of the DNA manipulation zone. The system also includes a controller programmed to close the first and second valves to prevent gas and liquid from flowing into or out of the DNA manipulation zone, and a computer-controlled heat source in thermal contact with the DNA manipulation zone.
    Type: Grant
    Filed: October 30, 2007
    Date of Patent: September 25, 2012
    Assignee: HandyLab, Inc.
    Inventors: Kalyan Handique, Gene Parunak
  • Patent number: 8268571
    Abstract: The present invention, provides a flow cytometry apparatus for the detection of particles from a plurality of samples comprising: means for moving a plurality of samples comprising particles from a plurality of respective source wells into a fluid flow stream; means for introducing a separation gas between each of the plurality of samples in the fluid flow stream; and means for selectively analyzing each of the plurality of samples for the particles. The present invention also provides a flow cytometry method employing such an apparatus.
    Type: Grant
    Filed: August 11, 2011
    Date of Patent: September 18, 2012
    Assignee: STC.UNM
    Inventors: Larry A Sklar, Bruce Edwards, Frederick Kuckuck
  • Patent number: 8268633
    Abstract: A method for conducting a broad range of biochemical analyses or manipulations on a series of nano- to subnanoliter reaction volumes and an apparatus for carrying out the same are disclosed. The invention is implemented on a fluidic microchip to provide high serial throughput. In particular, the disclosed device is a microfabricated channel device that can manipulate nanoliter or subnanoliter reaction volumes in a controlled manner to produce results at rates of 1 to 10 Hz per channel. The reaction volumes are manipulated in serial fashion analogous to a digital shift register. The invention has application to such problems as screening molecular or cellular targets using single beads from split-synthesis combinatorial libraries, screening single cells for RNA or protein expression, genetic diagnostic screening at the single cell level, or performing single cell signal transduction studies.
    Type: Grant
    Filed: April 5, 2007
    Date of Patent: September 18, 2012
    Assignee: UT-Battelle, LLC
    Inventors: J. Michael Ramsey, Stephen C. Jacobson
  • Patent number: 8263023
    Abstract: A microfluidic system and method for sorting cell clusters, and for the continuous and automated encapsulation of the clusters, once sorted, in capsules of sizes suitable for those of these sorted clusters is provided. The microfluidic system comprises a substrate in which a microchannel array comprising a cell sorting unit is etched and around which a protective cover is bonded, and the sorting unit comprises deflection means capable of separating, during the flow thereof, relatively noncohesive cell clusters, each of size ranging from 20 ?m to 500 ?m and of 20 to 10 000 cells approximately, such as islets of Langerhans, at least two sorting microchannels arranged in parallel at the outlet of said unit being respectively designed so as to transport as many categories of sorted clusters continuously to a unit for encapsulation of the latter, also formed in said array.
    Type: Grant
    Filed: May 8, 2009
    Date of Patent: September 11, 2012
    Assignee: Commissariat a l'Energie Atomique
    Inventors: Sophie Le Vot, Jean Berthier, Florence Rivera
  • Patent number: 8252539
    Abstract: A microfluidic device for analyzing and/or sorting biological materials (e.g., molecules such as polynucleotides and polypeptides, including proteins and enzymes; viruses and cells) and methods for its use are provided. The device and methods of the invention are useful for sorting particles, e.g. virions. The invention is also useful for high throughput screening, e.g. combinatorial screening. The microfluidic device comprises a main channel and an inlet region in communication with the main channel at a droplet extrusion region. Droplets of solution containing the biological material are deposited into the main channel through the droplet extrusion region. A fluid different from and incompatible with the solution containing the biological material flows through the main channel so that the droplets containing the biological material do not diffuse or mix.
    Type: Grant
    Filed: October 8, 2007
    Date of Patent: August 28, 2012
    Assignee: California Institute of Technology
    Inventors: Stephen R. Quake, Todd Thorsen
  • Patent number: 8173431
    Abstract: A multi-tier approach for use in a mailroom for detecting bio-threats conveyed by mail. In a first tier procedure, mail entering the mailroom is continuously automatically screened at a mail screening station to detect a potential bio-threat contamination. If a potential bio-threat is detected, an alarm signal is generated, a sample of the potential bio-threat is collected, and a second tier procedure is initiated. The second tier procedure uses a manual test, such as a nucleic acid amplification and detection assay to detect any of a plurality of different specific bio-threats in the sample. If a specific bio-threat is found, appropriate steps are taken to limit spread of the bio-threat and exposure of personnel. A third tier procedure uses a manual assay to check for a specific toxin in background samples collected over a predefined time interval at each mail screening station and in the air handling system for the mailroom.
    Type: Grant
    Filed: November 9, 2006
    Date of Patent: May 8, 2012
    Assignee: FLIR Systems, Inc.
    Inventor: Charles J. Call
  • Patent number: 8137623
    Abstract: The present invention provides an apparatus for analyzing easily not only the overall taste of a sample but also what kind of components, synergetic effects and diminishing effects contribute to the taste. A sample to be analyzed is injected into a mobile phase by a liquid sending pump, and the sample is sent to a taste detector by way of a blending means and valves. Then, detection signals are obtained by taste sensors on the taste detector. The sample sent through the taste detector is introduced into a column by way of a 6-port-2-position valve, temporally separated into components and eluted from the column. After each component is detected by a UV detector, the eluted liquid is sent to the taste detector once more, and detection signals which reflect the tastes of each sample component. When analyzing how the taste changes by an additive, send the additive to the blending means and blend it with the sample.
    Type: Grant
    Filed: June 6, 2007
    Date of Patent: March 20, 2012
    Assignees: Shimadzu Corporation, Takasago International Corporation
    Inventors: Yosuke Iwata, Tsuyoshi Kobayashi
  • Patent number: 8124413
    Abstract: The invention relates to continuous flow systems, in particular thermocyclers for the automated and continuous cycling of fluid between a plurality of temperature zones in the amplification of nucleic acids. The invention also relates to an improved sample port for introducing a volume of a liquid sample into a continuous flow system.
    Type: Grant
    Filed: February 2, 2007
    Date of Patent: February 28, 2012
    Assignee: Corbett Life Science Pty Ltd
    Inventors: Keith Stanley, John Corbett
  • Patent number: 8119068
    Abstract: A fluid content monitor including a cuvette, a colorimeter adapted to generate a signal indicative of contents of a fluid sample contained in the cuvette, a container for holding a reagent, and a pump assembly for delivering reagent from the container to the cuvette. The pump assembly includes a tube extending from the container to the cuvette, check valves preventing reverse flow in the tube, and a hammer driven by a solenoid for repetitively compressing the tube to pump reagent to the cuvette. The cuvette can be removed for cleaning and replacement.
    Type: Grant
    Filed: July 28, 2010
    Date of Patent: February 21, 2012
    Assignee: HF Scientific, Inc.
    Inventors: Rowan Connelly, Joel Leal
  • Patent number: 8062903
    Abstract: Methods and devices are provided for overcoming detrimental diffusive effects in a sample liquid stream by forming segmented liquid bodies (e.g., droplets) from a sample liquid stream in an immiscible liquid stream. The liquid bodies are formed at the intersection of a channel providing the sample liquid stream and a channel providing the immiscible liquid stream. The formed liquid bodies compartmentalize the portion of the sample liquid stream from which the liquid bodies are formed, thus minimizing the detrimental effects of diffusion that occur in a continuous liquid stream.
    Type: Grant
    Filed: March 3, 2009
    Date of Patent: November 22, 2011
    Assignee: University of Washington
    Inventors: Daniel T. Chiu, John Scott Edgar
  • Patent number: 8053249
    Abstract: A method is provided for pumping fluid through a channel of a microfluidic device. The channel has an input port and an output port. The channel is filled with fluid and a pressure gradient is generated between the fluid at the input port and the fluid at the output port. As a result, fluid flows through the channel towards the output port.
    Type: Grant
    Filed: March 12, 2007
    Date of Patent: November 8, 2011
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: David J. Beebe, Jay W. Warrick, Michael W. Toepke, Ivar Meyvantsson, Glenn M. Walker