With Formation Of A Segmented Stream Patents (Class 436/53)
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Patent number: 6426225Abstract: A modular vial autosampler has a storage area for vials containing samples to be analyzed and at least one modular sampling station. A vial transfer mechanism includes an arm having a gripper that lifts a sample vial from the storage section, and the arm moves it to a station for identification and then to a sampling station, and under central control activates the sampling station for obtaining a sample for analysis. The vial transfer mechanism gripper is movable in X, Y, and Z directions to capture and move a selected vial and includes an alignment guide for the vials. Potentiometers are used for providing signals indicating arm position and the control is provided with updated information for calibration of the potentiometers and also updated position information for the arm relative to a fixed home position is obtained.Type: GrantFiled: August 16, 1999Date of Patent: July 30, 2002Assignee: Tekmar CompanyInventors: Edmund T. Lewis, Thomas B. Green, Harry W. Schmidt, Larry J. DaPrato, Greg J. Herman, Michael A. Hill
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Patent number: 6348354Abstract: A method and apparatus for controlling a stream of liquid and air segments wherein the liquid and air segments are selectively aspirated into a first fluid conduit in a plurality of cycles, each cycle beginning with the aspiration of a first air segment and ending with the aspiration of a final air segment. The liquid and air segments are then transferred from the first fluid conduit to a second fluid conduit. The volume of the final air segment of each cycle is then adjusted after the final air segment has moved into the second fluid conduit. Next, the liquid segments and the air segments of each cycle are transferred from the second fluid conduit to a third fluid conduit. The volume of the first air segment of each cycle is then adjusted after the first air segment has moved into the third fluid conduit.Type: GrantFiled: July 6, 1998Date of Patent: February 19, 2002Assignee: Bayer CorporationInventors: Robert Adolfsen, Paul Gherson, David Lightbody
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Patent number: 6333197Abstract: A method for fractionated distribution of a blood sample, includes the steps of: a) drawing up a blood sample into a needle; b) distributing via the needle an aliquot corresponding to a specific fraction of the blood sample drawn up, into a flow of a reagent, to thereby produce a mixture of the aliquot and of the reagent; c) collecting the mixture in a mixing and/or measuring receptacle; and d) repeating steps b) and c) at least once to distribute a further aliquot corresponding to a further specific fraction of the blood sample, into a further flow of reagent.Type: GrantFiled: October 13, 1998Date of Patent: December 25, 2001Assignee: A B XInventors: Roger Le Comte, Henri Champseix
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Patent number: 6326211Abstract: The present invention provides a miniaturized integrated nucleic acid diagnostic device and system. The device of the invention is generally capable of performing one or more sample acquisition and preparation operations, in combination with one or more sample analysis operations. For example, the device can integrate several or all of the operations involved in sample acquisition and storage, sample preparation and sample analysis, within a single integrated unit. The device is useful in a variety of applications, and most notably, nucleic acid based diagnostic applications and de novo sequencing applications.Type: GrantFiled: March 10, 2000Date of Patent: December 4, 2001Assignee: Affymetrix, Inc.Inventors: Rolfe C. Anderson, Robert J. Lipshutz, Richard P. Rava, Stephen P. A. Fodor
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Publication number: 20010036672Abstract: The present invention provides a miniaturized integrated nucleic acid diagnostic device and system. The device of the invention is generally capable of performing one or more sample acquisition and preparation operations, in combination with one or more sample analysis operations. For example, the device can integrate several or all of the operations involved in sample acquisition and storage, sample preparation and sample analysis, within a single integrated unit. The device is useful in a variety of applications, and most notably, nucleic acid based diagnostic applications and de novo sequencing applications.Type: ApplicationFiled: December 31, 2000Publication date: November 1, 2001Inventors: Rolfe C. Anderson, Robert J. Lipshutz, Richard P. Rava, Stephen P. A. Fodor
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Patent number: 6297061Abstract: This invention provides a method and apparatus for detecting the presence of analyte particles in a sample fluid also comprising larger particles, particularly blood. It exploits diffusion to provide simultaneous filtering of the larger particles and reaction of the analyte particles. A sample stream and a reagent stream join on the upstream end of a laminar flow reaction channel and flow in adjacent laminar streams. The reagents can be in solution or immobilized on a bead. The analyte particles diffuse from the sample stream into the reagent stream, leaving behind the larger particles in the residual sample stream. In the reagent stream the analyte particles react with reagent particles and form product particles, thereby creating a product stream. At the downstream end of the reaction channel, the residual sample stream and the product stream are divided. The product particles are then detected, preferably optically, in the product stream.Type: GrantFiled: February 10, 2000Date of Patent: October 2, 2001Assignee: University of WashingtonInventors: Caicai Wu, Bernhard Weigl, Margaret A. Kenny, Paul Yager
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Patent number: 6277641Abstract: The present invention provides a microfabricated sensor and a method capable of rapid simultaneous measurement of multiple analytes in a fluid sample. The sensor is inexpensive, disposable and portable, and requires only microliters of sample, a particular advantage with precious fluids such as blood. The sensor utilizes diffusion between layered laminar streams rather than side by side streams. This allows multiple side by side channels for simultaneous detection of multiple analytes. In the sensor, a sample stream and a carrier stream flow in layers, one on top of the other, and one or more reagents are introduced to the bottom of the carrier stream through either a fluid or a solid reagent inlet. The reagent contains reagent particles which, in the presence of the analyte, have a detectable change in a property. The analyte diffuses into the carrier stream where it interacts with reagent particles and is detected by optical, electrochemical or other devices.Type: GrantFiled: November 17, 1999Date of Patent: August 21, 2001Assignee: University of WashingtonInventor: Paul Yager
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Patent number: 6261847Abstract: The sample dilution module includes an oil filled aspiration probe and mixing chamber that communicate with each other while the mixing chamber is maintained at an angle of approximately 5 to 45 degrees below a horizontal axis. The aspiration probe is maintained in a vertical orientation. A micro-bubble is aspirated between separate aspirations of test sample and diluent to prevent contact between the sample and diluent in the aspiration probe. The test sample and diluent are then drawn from the vertically oriented aspiration probe into the mixing chamber. After the sample and diluent are in the mixing chamber the micro-bubble of air can migrate away from the test sample and diluent to enable the test sample and diluent to contact each other. Mixing of the test sample and diluent is accomplished by moving the test sample and diluent back and forth in the mixing chamber without physical agitation of the mixing chamber and without any mixing element in the mixing chamber.Type: GrantFiled: July 10, 1998Date of Patent: July 17, 2001Assignee: Bayer CorporationInventors: Robert W. Eherts, Evandro S. Denunzio
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Patent number: 6221677Abstract: This invention provides a method and apparatus for detecting the presence of analyte particles in a sample fluid also comprising larger particles, particularly blood. It exploits diffusion to provide simultaneous filtering of the larger particles and reaction of the analyte particles. A sample stream and a reagent stream join on the upstream end of a laminar flow reaction channel and flow in adjacent laminar streams. The reagents can be in solution or immobilized on a bead. The analyte particles diffuse from the sample stream into the reagent stream, leaving behind the larger particles in the residual sample stream. In the reagent stream the analyte particles react with reagent particles and form product particles, thereby creating a product stream. At the downstream end of the reaction channel, the residual sample stream and the product stream are divided. The product particles are then detected, preferably optically, in the product stream.Type: GrantFiled: June 18, 1999Date of Patent: April 24, 2001Assignee: University of WashingtonInventors: Caicai Wu, Bernhard Weigl, Margaret A. Kenny, Paul Yager
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Patent number: 6218193Abstract: A high precision, small volume fluid processing system employs open ended capillary tubes to meter, aliquot and mix small volumes of sample fluid and reagents. The system has an automatic mechanism for moving the capillary tubes as well as automated sub-systems for incubating and mixing fluids within the capillary tubes.Type: GrantFiled: June 30, 1998Date of Patent: April 17, 2001Assignee: University of WashingtonInventors: Ray Kraft, Neal A. Friedman, Deirdre Meldrum, Ronald Seubert
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Patent number: 6171865Abstract: A reference sensor system is provided for detecting the presence and/or measuring the concentration of analyte particles in a sample stream. The system includes: a) a laminar flow channel; b) three or more inlet means in fluid connection with the laminar flow channel for respectively conducting into the laminar flow channel (1) an indicator stream which may include an indicator substance which indicates the presence of the analyte particles, (2) the sample stream, and (3) a reference stream, which can be a control stream and/or internal standard stream; and, c) wherein the laminar flow channel has a depth and/or width sufficiently small to allow laminar flow of said streams and a length sufficient to allow particles of the analyte to diffuse into the indicator stream to form a detection area. Branching channels may be provided as outlet means for conducting the streams out of the laminar flow channel.Type: GrantFiled: August 4, 1999Date of Patent: January 9, 2001Assignee: University of WashingtonInventors: Bernhard H. Weigl, Mark R. Holl, Diane Zebert, Margaret Kenny, Caicai Wu
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Patent number: 6159739Abstract: The present invention provides a sheath flow module made from a first plate of material having formed therein a laminar fluid flow channel; at least two inlets, each inlet joining the laminar flow channel at a junction, the first inlet junction being wider than the second inlet junction, and an outlet from the flow channel. A second plate, e.g. a transparent cover plate, seals the module and allows for optical measurements. A first inlet allows for introduction of a first fluid into the flow channel. The first fluid is the sheath fluid. A second inlet allows for introduction of a second fluid into the sheath fluid while it is flowing through the flow channel. The second fluid is the center fluid. Because the second inlet junction is narrower than the first inlet junction, the center fluid becomes surrounded on both sides by the sheath fluid. After all fluids have been introduced and sheath flow has been achieved, the depth of the flow channel can be decreased, leading to vertical hydrodynamic focusing.Type: GrantFiled: March 26, 1997Date of Patent: December 12, 2000Assignee: University of WashingtonInventors: Bernhard Weigl, Paul Yager, James P. Brody
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Patent number: 6133044Abstract: A droplet forming flow cytometer system allows high speed processing without the need for high oscillator drive powers through the inclusion of an oscillator or piezoelectric crystal such as within the nozzle volume or otherwise unidirectionally coupled to the sheath fluid. The nozzle container continuously converges so as to amplify unidirectional oscillations which are transmitted as pressure waves through the nozzle volume to the nozzle exit so as to form droplets from the fluid jet. The oscillator is directionally isolated so as to avoid moving the entire nozzle container so as to create only pressure waves within the sheath fluid. A variation in substance concentration is achieved through a movable substance introduction port which is positioned within a convergence zone to vary the relative concentration of substance to sheath fluid while still maintaining optimal laminar flow conditions. This variation may be automatically controlled through a sensor and controller configuration.Type: GrantFiled: April 16, 1996Date of Patent: October 17, 2000Assignee: University of WashingtonInventor: Ger Van den Engh
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Patent number: 6130098Abstract: The movement and mixing of microdroplets through microchannels is described employing microscale devices, comprising microdroplet transport channels, reaction regions, electrophoresis modules, and radiation detectors. The discrete droplets are differentially heated and propelled through etched channels. Electronic components are fabricated on the same substrate material, allowing sensors and controlling circuitry to be incorporated in the same device.Type: GrantFiled: September 26, 1997Date of Patent: October 10, 2000Assignee: The Regents of the University of MichiganInventors: Kalyan Handique, Bishnu Gogoi, Mark A. Burns, Carlos H. Mastrangelo
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Patent number: 6040186Abstract: A modular vial autosampler has a storage area for vials containing samples to be analyzed and at least one modular sampling station. A vial transfer mechanism lifts a sample vial from the storage section, moves it to a station for identification and then to a sampling station, and under central control activates the sampling station for obtaining a sample for analysis. The vial transfer mechanism is movable in x, y, and z directions to capture and move a selected vial. The autosampler has a series of valves operable under central control to selectively introduce two different standards into the sample, and after obtaining the sample, for rinsing and purging the conduits or lines and needles to reduce sample carryover. The modular vial autosampler includes controls to selectively sample either a gas or a liquid using many of the same components.Type: GrantFiled: April 24, 1997Date of Patent: March 21, 2000Assignee: Tekmar CompanyInventors: Edmund T. Lewis, Thomas B. Green
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Patent number: 5981289Abstract: A hydrogen sulfide analyzer that continuously samples waste water from a waste stream or reservoir and measures the concentration of purgeable H.sub.2 S present (H.sub.2 SP) This information, when combined with the volume of water present, provides a control quality signal that regulates the feed rate of the destructor chemical into the waste stream. This results in chemical savings for the user. A second result is the reduction in odor complaints and the corrosion problems associated with H.sub.2 S emissions. The analyzer measures only the purgeable H.sub.2 S contained in the liquid sample. The analyzer violently agitates the sample containing dissolved H.sub.2 S in solution to simulate actual conditions at points of agitation in the waste water stream. It also provides nearly optimal partial pressure conditions for the H.sub.2 S to exit the solution as a free gas. Any H.sub.Type: GrantFiled: October 13, 1998Date of Patent: November 9, 1999Assignee: Isco, Inc.Inventors: Paul G. Wright, David J. Shannon, Lowell R. Nickolaus, Randy J. Forman, Clifford L. McDonald, Bennett K. Horenstein
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Patent number: 5968835Abstract: To estimate nitric acid ions and nitrous acid ions in a sample solution, a method is proposed which comprises the steps of (a) feeding both the sample solution and a reducing agent to an acidic solution which is flowing in a thin pipe; (b) positively mixing the sample solution and the reducing agent and thus reacting the same in a given portion of the pipe; (c) leading the reacted solution to a gas/liquid separator thereby to separate a gaseous phase from the reacted solution; and (d) estimating the nitric acid ions and nitrous acid ions in the gaseous phase.Type: GrantFiled: February 27, 1997Date of Patent: October 19, 1999Assignee: Kabushiki Kaisha MeidenshaInventors: Toyoaki Aoki, Hiroshi Tsugura
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Patent number: 5965450Abstract: A system for measuring the amount of cyanide species in a sample, namely total cyanide, dissociable cyanide, complex cyanide and thiocyanate, by using a single apparatus for simultaneous ultraviolet irradiation and distillation. The system which can be used with either segmented flow or flow injection sampling utilizes filter components which pass only lower frequency ultraviolet radiation interposed between a photoillumination source and the alkaline or acidified sample to first breakdown the strong complex cyanide from the sample without dissociation of thiocyanates. The system then distills the irradiated sample in the same apparatus by utilizing heat emitted from the photoillumination source to recover the total cyanides for measurement. Dissociable cyanides, as well as thiocyanate, are measured using the same apparatus by altering the filter components.Type: GrantFiled: July 2, 1998Date of Patent: October 12, 1999Inventor: Nabih P. Kelada
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Patent number: 5948684Abstract: A reference T-sensor system is provided for detecting the presence and/or measuring the concentration of analyte particles in a sample stream. The system includes: a) a laminar flow channel; b) three or more inlet means in fluid connection with the laminar flow channel for respectively conducting into the laminar flow channel (1) an indicator stream which may include an indicator substance which indicates the presence of the analyte particles, (2) the sample stream, and (3) a reference stream, which can be a control stream and/or internal standard stream; c) wherein the laminar flow channel has a depth and/or width sufficiently small to allow laminar flow of the streams and a length sufficient to allow particles of the analyte to diffuse into the indicator stream to form a detection area; and d) outlet means for conducting the streams out of the laminar flow channel preferably to form a single mixed stream.Type: GrantFiled: July 25, 1997Date of Patent: September 7, 1999Assignee: University of WashingtonInventors: Bernhard H. Weigl, Mark R. Holl, Diane Zebert, Margaret Kenny, Caicai Wu
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Patent number: 5948360Abstract: A modular vial autosampler has a storage area for vials containing samples to be analyzed and at least one modular sampling station. A vial transfer mechanism includes an arm having a gripper that lifts a sample vial from the storage section, and the arm moves it to a station for identification and then to a sampling station, and under central control activates the sampling station for obtaining a sample for analysis. The vial transfer mechanism gripper is movable in X, Y, and Z directions to capture and move a selected vial and includes an alignment guide for the vials. Potentiometers are used for providing signals indicating arm position and the control is provided with updated information for calibration of the potentiometers and also updated position information for the arm relative to a fixed home position is obtained.Type: GrantFiled: August 29, 1997Date of Patent: September 7, 1999Assignee: Tekmar CompanyInventors: Prabhakar P. Rao, Thomas B. Green, Harry W. Schmidt, Larry J. DaPrato, Greg J. Herman, Michael A. Hill
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Patent number: 5935522Abstract: An apparatus particularly suited for subjecting biological samples to any necessary sample preparation tasks, subjecting the sample to rapid thermal cycling, and then subjecting the sample to subsequent on-line analysis using one or more of a number of analytical techniques. The apparatus includes a chromatography device including an injection means, a chromatography pump, and a chromatography column. In addition, the apparatus also contains a capillary electrophoresis device consisting of a capillary electrophoresis column with an inlet and outlet end, a means of injection, and means of applying a high voltage to cause the differential migration of species of interest through the capillary column.Type: GrantFiled: June 30, 1997Date of Patent: August 10, 1999Assignee: University of Utah Research FoundationInventors: Harold P. Swerdlow, Carl T. Wittwer
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Patent number: 5895764Abstract: A flow cytometry method and system to regulate both the sheath and sample streams during the period of sample injection. The period of turbulence between the streams is reduced to approximately 100 milliseconds thereby allowing for focused measurement of cell associated fluorescence.Type: GrantFiled: November 24, 1997Date of Patent: April 20, 1999Assignee: University of New MexicoInventors: Larry A. Sklar, Larry C. Seamer
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Patent number: 5854072Abstract: The invention relates to a method and an apparatus for determining product-specific quality parameters of liquid, especially liquid (14) containing alcohol and held in a container (12) such as a bottle or can, the quality parameters being determined by means of sensors (22, 24, 26). To enable performing measurements with simply provisions and without complicated specimen preparation, and avoiding cross-sensitivities, it is proposed that the quality parameters of the liquid are determined under the conditions prevailing in the container, and liquid is initially delivered, under the pressure prevailing in the container, to a temperature unit (32) and adjusted to a temperature that corresponds to that of the sensors, and is then delivered to the sensors.Type: GrantFiled: August 6, 1996Date of Patent: December 29, 1998Inventors: Stephan Dittrich, Hubert Koukol, Robert Koukol
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Patent number: 5834314Abstract: Embodiments disclosed involve methods and constructions for metering, and in some instances combining, fluids. One such method includes fluidly connecting a source of first fluid to be metered with a pressure source capable of moving the first fluid with a first valve and a conduit. The first valve is operated such that first fluid moves in the conduit. The conduit is fluidly connected with a source of second fluid that has a fluid flow rate greater than the first fluid with a second valve. The second valve is operated such that the second fluid moves into the conduit. One construction for metering a fluid comprises a source of first fluid to be metered, a pressure source for moving the first fluid to be metered, and a first valve. A conduit fluidly connects the source of first fluid, the pressure source for moving the first fluid and the first valve such that operation of the first valve causes movement of the first fluid responsive to the pressure source.Type: GrantFiled: August 6, 1997Date of Patent: November 10, 1998Assignee: Abbott LaboratoriesInventors: James D. Gates, Jeffrey Y. Pan, Tung-Ming Huang, Donald Ver Lee, Alan J. Alcock, Conan K. N. Li, Karen A. Egan
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Patent number: 5744099Abstract: An apparatus and method is disclosed for transferring biological fluids such as blood and serum from closed containers to multiple receiving containers. The disclosed methods and apparatus perform the transfer of an accurate and precise amount of biological liquid without dilution of the biological liquid, and without splattering of the biological liquid on the walls of the receiving container. Furthermore, the method discloses how to obtain equal concentrations of white blood cells in multiple receiving containers characteristic of their concentration in the sealed container.Type: GrantFiled: September 15, 1995Date of Patent: April 28, 1998Assignee: Cytek Development Inc.Inventors: Eric S. Chase, Harvey L. Schulte
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Patent number: 5739036Abstract: An analyzer apparatus for analyzing components of a sample has a conduit and an electrochemical sensor in the conduit. The sample is passed through the conduit using a carrier liquid that is immiscible with the sample and non-wetting of the inner wall of the conduit to reduce instabilities in the apparatus due to undue fluid movement.Type: GrantFiled: August 15, 1997Date of Patent: April 14, 1998Assignee: Dade International Inc.Inventor: Norman Alfred Parris
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Patent number: 5668014Abstract: To estimate nitric acid ions and nitrous acid ions in a sample solution, a method is proposed which comprises the steps of (a) feeding both the sample solution and a reducing agent to an acidic solution which is flowing in a thin pipe; (b) positively mixing the sample solution and the reducing agent and thus reacting the same in a given portion of the pipe; (c) leading the reacted solution to a gas/liquid separator thereby to separate a gaseous phase from the reacted solution; and (d) estimating the nitric acid ions and nitrous acid ions in the gaseous phase.Type: GrantFiled: June 2, 1995Date of Patent: September 16, 1997Assignee: Kabushiki Kaisha MeidenshaInventors: Toyoaki Aoki, Hiroshi Tsugura
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Patent number: 5631167Abstract: Improved assay methods utilizing stable, enzymatically triggered chemiluminescent 1,2-dioxetanes with improved water solubility are provided. Assays are performed by a capsule chemistry analytical assay method wherein fluid capsules comprising discrete aqueous segments containing either a chemiluminescent dioxetane reagent or an activating agent separated by an oil-based isolating fluid are flowed through a conduit, and subsequently reacted to produce light.The improvement comprises using a dioxetane substituted with two or more water-solubilizing groups disposed on the dioxetane structure to provide the chemiluminescence. Compositions comprising such a dioxetane, a non-polymeric surfactant enhancer and optionally a fluorescer, provide enhanced chemiluminescence and eliminate the problem of reagent carryover when used in assays performed on capsule chemistry analytical systems.Type: GrantFiled: July 31, 1995Date of Patent: May 20, 1997Assignees: Bayer Corporation, Board of Governors Wayne State Univ.Inventors: Robert F. Adolfsen, Hashem Akhavan-Tafti, Renuka De Silva, A. Paul Schaap
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Patent number: 5630987Abstract: An apparatus for measuring the concentration of a contaminant in a fluid includes a measuring chamber in a fluid path, a first source of optical radiation of a predetermined wavelength adapted to direct the radiation through the measuring chamber, and a detector for detecting the radiation after passage through the measuring chamber. The fluid path also has a pump provided to urge a test fluid from a fluid source to the measuring chamber, the test fluid being subjected to containing the contaminant, a fluid processor to process the fluid to change an optical effect of the contaminant, an acid wash to inhibit the effect of precipitation of cations within the fluid path, a fluid bypass to bypass the fluid processor and a valve to alternately direct the fluid to the fluid processor and the bypass.Type: GrantFiled: January 24, 1995Date of Patent: May 20, 1997Assignee: British Technology Group LimitedInventors: Ronald Briggs, Kenneth T. V. Grattan, Zoheir Movaziz
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Patent number: 5612225Abstract: This invention provides a process for determining the nitrogen content of a sample involving (a) combusting the sample in a combustion reactor in the presence of oxygen, which oxygen is introduced into the combustion reactor in pulse form; (b) reducing the resulting combustion gases by flowing them in a flow of helium or other noble carrier gas through a Cu-containing reduction reactor; (c) performing the steps of (i) making anhydrous in a water trap the reduced gases, (ii) flowing the reduced gases through a gas-chromatographic column in order to carry out a gas-chromatographic separation of N.sub.2 from CH.sub.4 possibly present within the reduced gases, and (iii) removing CO.sub.2 from the reduced gases using a CO.sub.2 trap, with the proviso that step (i) immediately precedes step (ii); and (d) analyzing the resulting gases by detecting thermal conductivity.Type: GrantFiled: June 7, 1995Date of Patent: March 18, 1997Assignee: Fisons Instruments S.p.A.Inventors: Marco Baccanti, Paolo Magni
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Patent number: 5591644Abstract: A method for on-line assay in column chromatography of a property that requires appreciable time for sensitive measurement has the steps of separating the effluent into discrete fractions at equal time intervals, sequentially passing each fraction through each of a series of detectors, while preventing each fraction from mixing with another; and, for each fraction, calculating the assay from the information provided by each detector during the time interval the fraction passed through it.Type: GrantFiled: May 11, 1994Date of Patent: January 7, 1997Assignee: Albert Einstein College of Medicine of Yeshiva UniversityInventor: Arthur Karmen
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Patent number: 5576218Abstract: The method for thermal cycling of nucleic acid assays includes a blended fluid stream produced from a plurality of constant velocity, constant volume, constant temperature fluid streams wherein to provide a variable temperature, constant velocity, constant volume fluid stream which is introduced into a sample chamber for heating and cooling samples contained therein. By diverting and altering the ratio of the constant temperature fluid streams relative to one another, the blended fluid stream is rapidly variable in temperature, providing for almost instantaneous temperature change within the environment defined by the sample chamber.Type: GrantFiled: April 14, 1995Date of Patent: November 19, 1996Assignee: Abbott LaboratoriesInventors: Thomas F. Zurek, Kathleen A. Hanley, Curtis J. Pepe
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Patent number: 5550053Abstract: A method for calibrating an automatic chemical analyzer whereby an improved baseline value is calculated. The analyzer uses a photodetector to detect the amount of light passing through a flow cell and correlates this value to the amount of analyte present in a sample solution. In the preferred embodiment, silica is the analyte under investigation. A baseline solution of a specified (preferably zero) analyte concentration, or a sample solution of unknown analyte concentration, is introduced into a chemical module after chemical reagents have reacted with themselves to form a color complex. The resulting solution is passed to the photodetector which determines the amount of analyte present. This value corresponds to the analyte contaminants within the reagents and can be subtracted out by further calculations.Type: GrantFiled: January 5, 1995Date of Patent: August 27, 1996Assignee: SI Industrial Instruments, Inc.Inventor: Jerome Salpeter
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Patent number: 5547553Abstract: An improved mercury electrode for electrochemical analysis is formed by a small diameter thread of liquid mercury contained within an inert tube which, at one point along its length, has an short, fixed length of thin walled tubular semipermeable membrane surrounding and forming the electrodes' active surface in order to prevent or reduce fouling of the surface while allowing the mercury thread to be advanced through the membrane to expose a fresh active surface whenever desired.Type: GrantFiled: April 21, 1995Date of Patent: August 20, 1996Assignee: Bioanalytical Systems, Inc.Inventor: Husantha G. Jayaratna
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Patent number: 5547875Abstract: A method and apparatus for recalibrating analytical instruments such as atomic absorption and inductively coupled plasma spectrophotometers comprises the use of coupled piston pumps and valves in a fluid conduit system to obtain a sample value (h) during one half of a pump cycle and standard gradients (S1-S6) during the other half cycle. The piston pumps are actuated by cams of selected profiles to obtain a predetermined flow rate from each pump, the flow from each pump being selectively combined to construct standard gradients from two or more standard solutions and to effect autoranging of the standards in response to a previously determined sample value.Type: GrantFiled: January 10, 1995Date of Patent: August 20, 1996Assignee: Ionode Pty Ltd.Inventors: John D. Petty, Russell M. Peachey
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Patent number: 5506142Abstract: A system for cleaning a probe used in analytic equipment--for example, that used in analysis of body fluids--in order to reduce carry-over of materials from one sample to the next. A probe wash is provided in which the simultaneous introduction of pressurized air and water creates a turbulent flow including the use of a pressurized gas stream of short duration to blow the residue of the previous sample out of the probe prior to washing with additional diluent liquid. Also, a waste receptacle is provided which uses a filtered air vent and a liquid saturated material around the probe receiving opening to prevent the escape of aerosols from the receptacle.Type: GrantFiled: September 7, 1994Date of Patent: April 9, 1996Assignee: Dade International Inc.Inventors: Richard C. Mahaffey, John C. Mazza, Ronald N. Diamond
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Patent number: 5503994Abstract: In many separation techniques, such as field flow fractionation, liquid chromatography and electro-phoresis, chemical species form bands that migrate at different velocities. If the data-digitization rate and excitation intensity are both set to be optimal for the fastest migrating band, to compensate for different band velocities, both the data-digitization rate and the excitation intensity are decreased as a function of time by a factor equal to the migration time of the fastest migrating band to the separation time.Type: GrantFiled: October 8, 1993Date of Patent: April 2, 1996Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Jason B. Shear, Rajeev Dadoo, Harvey A. Fishman, Neil Shafer, Richard N. Zare
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Patent number: 5504010Abstract: A sample to be analyzed is taken from a flow line of a production process and transferred through a slender pipe to an analyzing device. The transfer is accomplished using a pressurized liquid with a segment gas being interposed between the sample and the pressurized liquid. The sample may be fed directly or after mixing with a diluting liquid in a mixing chamber. Samples may be taken at regular and constant time intervals and transferred to the analyzing device stably and over predetermined periods of time.Type: GrantFiled: March 20, 1995Date of Patent: April 2, 1996Assignee: Mitsui Petrochemical Industries, Ltd.Inventors: Toshiharu Mitani, Yasuo Miyoshi, Yoshiro Hisatomi
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Patent number: 5487981Abstract: An apparatus for and a method of on-line detecting and assessing, in situ, he amount and characterization of microbial trace contamination in an associated ultra-pure water system is disclosed. The apparatus comprises, inter alia, a monitor/detector portion and an assessment/identification portion. The monitor/detector portion includes a contamination mass detector which nondestructively senses the presence of microbial biofilms in real time when grown upon the surface of a quartz crystal wafer portion of the contamination mass detector. The assessment/identification portion is configured to provide quantitative analysis of the microbial biofilms formations based on signature biomarker cellular components. A programmable controller is configured to integrate these and other portions into an automated apparatus, which is capable of providing real time chemical countermeasures to prevent the potential deleterious effect of the microbial trace contamination uncovered.Type: GrantFiled: July 30, 1993Date of Patent: January 30, 1996Assignee: The United States of America as represented by the Secretary of the NavyInventors: David E. Nivens, James Q. Chambers, David C. White
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Patent number: 5477468Abstract: The concentration analyzer of the present invention is used to determine the cross-sectional concentration at multiple locations within a fluid line, such as an artery or vein, of dissolved substances within a moving fluid flowing through the vein which fluid consists of a first solution of unknown concentration.Type: GrantFiled: January 14, 1994Date of Patent: December 19, 1995Assignee: Project CDInventors: Philip Shapiro, Julian D. Warhurst
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Patent number: 5468642Abstract: A device for monitoring the boron content of the borophosphosilicate, BPSG. The BPSG is obtained by the oxidation of silane, of diborane and of phosphine in a reactor, starting with a first, diborane/silane gas mixture and a second, phosphine/silane gas mixture by monitoring the ratio .rho.=diborane/(diborane+silane) of the mixture resulting from the mixing of the first and second gas mixtures. Monitoring of the boron content is obtained by measuring the density of the first mixture, determining the amount of residual hydrogen in the first mixture and modulating the mass flow rate of the first mixture in response to the calculated ratio .rho..Type: GrantFiled: March 17, 1995Date of Patent: November 21, 1995Assignee: Matra MHS of FranceInventor: Alain Y. P. Charpentier
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Patent number: 5456835Abstract: A filter and a hollow-fiber or flat-sheet membrane for removing hemoglobin from whole blood or blood fractions are disclosed. The filter comprises a laid textile web which has been modified to attach a ligand for hemoglobin. The membrane comprises a polyethersulfone membrane that has been similarly modified. Methods for removing hemoglobin, cellular components and debris from blood are also disclosed.Type: GrantFiled: November 8, 1993Date of Patent: October 10, 1995Assignee: Hemasure, Inc.Inventors: Franco Castino, Abdul R. M. Azad, Eric K. Lee
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Patent number: 5441893Abstract: A method for monitoring the boron content of the borophosphosilicate, BPSG. The BPSG is obtained by the oxidation of silane, of diborane and of phosphine in a reactor, starting with a first, diborane/silane gas mixture and a second, phosphine/silane gas mixture by monitoring the ratio .rho.=diborane/(diborane+silane) of the mixture resulting from the mixing of the first and second gas mixtures. Monitoring of the boron content is obtained by measuring the density of the first mixture, determining the amount of residual hydrogen in the first mixture and modulating the mass flow rate of the first mixture in response to the calculated ratio .rho..Type: GrantFiled: April 4, 1994Date of Patent: August 15, 1995Assignee: Matra MHSInventor: Alain Y. P. Charpentier
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Patent number: 5399497Abstract: An analysis method and apparatus comprises feeding a stream of fluid capsules in a fluid conduit for flow therethrough by forming a film of isolating liquid on an inner surface of the conduit and randomly accessing each of a plurality of liquids selected from a sample liquid, reagents and a magnetic particle suspension to form a plurality of isolated segments from the plurality of liquids in each capsule in the conduit. The fluid capsules flow in at least one longitudinal direction of the conduit and the suspended magnetic particles are selectively magnetically retained from one segment in one capsule at a given location for placement of the magnetic particles in another segment in the one capsule during the flow of the one capsule through the conduit. The fluid capsules are then measured in the conduit.Type: GrantFiled: August 13, 1993Date of Patent: March 21, 1995Assignee: Miles, Inc.Inventors: Anand Kumar, Paul Gherson, Milt Pelavin
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Fluid sample collection and delivery system and methods particularly adapted for body fluid sampling
Patent number: 5380665Abstract: There is disclosed apparatus for transporting a sample of body fluid such as blood from a first location to a remote test site location. The apparatus operates in conjunction with a fluid manifold module which has a first sample input port, a second washing fluid input port and a third input port for receiving air. There are tubes coupled to said first, second and third input ports which are coupled together and coupled to a sample output line which extends from the first location to the remote location. The manifold contains check valves with one valve for blood and one valve for washing fluid and a diaphragm pump for air. There are logic controls which selectively operate the valves and pump to allow the sample line to receive a sample separated by air barriers from washing fluid solution. In this manner, a predetermined volume of sample is transported from the first location to the remote test site location.Type: GrantFiled: January 27, 1993Date of Patent: January 10, 1995Assignee: International Technidyne CorporationInventors: Robert F. Cusack, Michael D. Mintz -
Patent number: 5376550Abstract: Disclosed is a method and apparatus for sampling and determining the presence of certain substances, such as residues of contaminants in containers. The method includes steps of: injecting compressed air into said containers in order to displace at least a portion of the contents thereof; evacuating a sample of the container contents so displaced by applying suction thereto; and analyzing the sample evacuated to determine the presence or absence of the certain residues therein. The compressed air is injected through a nozzle into an opening in the containers to displace a portion of the container contents and form a sample cloud outside of the container. The sample cloud is then at least partially evacuated by suction and the sample is analyzed for the presence of contaminants such as nitrogen containing compounds or hydrocarbons. In one embodiment about 90% of the sample evacuated is diverted from the analyzer and recirculated into the air injector.Type: GrantFiled: September 18, 1992Date of Patent: December 27, 1994Assignee: The Coca-Cola CompanyInventors: David H. Fine, Freeman W. Fraim, Stephen J. MacDonald, Kenneth M. Thrash, Jr.
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Patent number: 5352611Abstract: A method and apparatus for sampling and determining the presence of certain substances, such as residues of contaminants in containers. The method includes steps of: injecting compressed air into said containers in order to displace at least a portion of the contents thereof; evacuating a sample of the container contents so displaced by applying suction thereto; and analyzing the sample evacuated to determine the presence or absence of the certain residues therein. The compressed air is injected through a nozzle into an opening in the containers to displace a portion of the container contents and form a sample cloud outside of the container. The sample cloud is then at least partially evacuated by suction and the sample is analyzed for the presence of contaminants such as nitrogen containing compounds or hydrocarbons. In one embodiment about 90% of the sample evacuated is diverted from the analyzer and recirculated into the air injector.Type: GrantFiled: June 1, 1992Date of Patent: October 4, 1994Assignee: The Coca-Cola CompanyInventors: David H. Fine, W. Fraim Freeman, Stephen J. MacDonald, Kenneth M. Thrash, Jr.
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Patent number: 5312757Abstract: A syringe is depressurized to suck a slight amount of air into the tip portion of a probe of a probe assembly, forming an air layer. Then, the syringe is depressurized again to suck into the probe assembly a sample to be discharged into a reaction container. Accordingly, the target sample to be discharged, the air layer and extruding water are placed into the probe assembly in the named order from the tip portion of the probe.Type: GrantFiled: April 21, 1992Date of Patent: May 17, 1994Assignee: Olympus Optical Co., Ltd.Inventors: Sinya Matsuyama, Takashi Yamada
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Patent number: 5279146Abstract: A method and apparatus for measuring particulates in a combustion gas is accomplished by dividing the combustion gas into a first and second sample gas. The first sample gas is filtered to remove particulates to create a reference gas sample. A combustion furnace can heat both the reference gas sample and the sample gas to oxidize any particulates in the sample gas. A gas analyzer that is connected to the furnace can detect the gas components in, respectively, the reference gas sample and the sample gas, and a comparison of the detected results can determine the concentration of particulates.Type: GrantFiled: August 5, 1992Date of Patent: January 18, 1994Assignee: Horiba Ltd.Inventors: Ichiro Asano, Kennosuke Kojima, Tokihiro Tsukamoto, Hiroji Kohsaka
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Patent number: 5268300Abstract: Apparatus and method for practice in an over-the-road vehicle to determine whether effluent water released in an operating water treatment system (plant installation) can be adequately treated to remove contaminants so the effluent may be reused at the plant rather than discharged to the environment with inimical effect. The frame of the vehicle supporting a plurality of clean holding tanks, a plurality of functionally different water treating units, an analysis laboratory, a pilot cooling tower simulator, and a pilot boiler simulator. The effluent is transferred from one of the holding tanks to at least one of the water treating units to reduce the contaminant level. When the contaminant level has been reduced to a level appropriate for reuse at the plant, the effluent is transferred to one of the simulator apparatus for evaluation of the treated water in the corresponding plant process.Type: GrantFiled: December 8, 1992Date of Patent: December 7, 1993Assignee: Nalco Chemical CompanyInventors: William D. Latura, Theodore A. Voruz, Abram R. Glazer