Carrier Is Water Suspendible Particles Patents (Class 436/534)
  • Patent number: 9939433
    Abstract: Latex particles for a particle agglutination assay, with which it is possible to carry out high-sensitivity assay while highly suppressing a non-specific reaction are disclosed. The latex particles being obtained by emulsion polymerization with a polymerizable monomer having a phenyl group, and a polymerizable monomer having a phenyl group and a sulfonate in an aqueous medium including a nonionic surfactant at a concentration of 0.005 to 0.02 wt % based on the aqueous medium, the latex particles having an average particle size of 0.005 to 1.0 ?m.
    Type: Grant
    Filed: December 28, 2012
    Date of Patent: April 10, 2018
    Assignee: SEKISUI MEDICAL CO., LTD.
    Inventors: Shinichiro Kitahara, Yuki Takahashi
  • Patent number: 9568471
    Abstract: This invention relates to assays for a Plasmodium analyte in a liquid sample such as a body fluid. More particularly, the invention relates to a method and apparatus for the detection of a ligand in a body fluid such as urine or blood, which can diagnose malarial infection.
    Type: Grant
    Filed: January 25, 2006
    Date of Patent: February 14, 2017
    Assignee: The Johns Hopkins University
    Inventors: David J. Sullivan, Peter Scholl
  • Patent number: 9447232
    Abstract: A process for producing carrier polymer particles comprising covering the surface of organic polymer particles having a particle diameter of 0.1 to 20 micrometers with a saccharide by chemically bonding the organic polymers and the saccharide.
    Type: Grant
    Filed: February 8, 2013
    Date of Patent: September 20, 2016
    Assignee: JSR CORPORATION
    Inventors: Masayuki Takahashi, Tetsuo Fukuta, Kiyoshi Kasai, Toshihiro Ogawa, Satoshi Katayose, Kouji Tamori, Kinji Yamada
  • Patent number: 9201059
    Abstract: The invention relates to a microfluidic system comprising a microfluidic device having a first and a second opposite surfaces and an optical detector, the microfluidic device comprises a flow channel with a detection channel section having a length of at least about 1 mm, the microfluidic device comprises at least one aperture section comprising at least a part of said detection channel section and a transparent window into said detection channel section, the optical detector is arranged to determine at least one optical property of said aperture section as a function of time. The flow channel may have capillary dimensions and/or it may wholly or fully be arranged to drive a fluid flow by applying external forces. The microfluid device may be used to determine various properties of a sample fluid, for example it may be used to determine a samples coagulation properties and/or reactions of one or more components in a fluid sample as a function of time.
    Type: Grant
    Filed: March 13, 2009
    Date of Patent: December 1, 2015
    Assignee: SCANDINAVIAN MICRO BIODEVICES APS
    Inventors: Niels Kristian Bau-Madsen, Jacques Jonsmann, Bent Overby, Christianus Berendsen
  • Patent number: 8993345
    Abstract: A method of producing functional molecule-containing silica nanoparticles on which a biomolecule is bonded, containing the steps of: allowing silica nanoparticles containing a functional molecule and having a thiol group on a surface thereof to coexist with a linker molecule having a maleimido group and a carboxyl group in an aprotic solvent, thereby allowing formation of a thioether bond between the thiol group and the maleimido group, and obtaining functional molecule-containing silica nanoparticles on which the linker molecule is bonded; and allowing the functional molecule-containing silica nanoparticles on which the linker molecule is bonded to coexist with carbodiimide and a biomolecule having an amino group in an aqueous solvent, thereby allowing formation of an amide bond between the carboxyl group active esterified by the carbodiimide, and the amino group of the biomolecule.
    Type: Grant
    Filed: October 25, 2013
    Date of Patent: March 31, 2015
    Assignee: Furukawa Electric Co., Ltd.
    Inventors: Hideki Aizawa, Michio Ohkubo, Masataka Nishida
  • Patent number: 8987005
    Abstract: [PROBLEMS] To provide a reagent for measuring agglutination by using a reaction accelerator, which causes no spontaneous agglutination of receptor-sensitized carrier particles in the coexistence of these carrier particles, and a measurement method. [MEANS FOR SOLVING PROBLEMS] A reagent for measuring agglutination by using a specific amine compound, whereby aggregation based on a specific reaction can be accelerated without causing spontaneous agglutination of carrier particles, and measurement method.
    Type: Grant
    Filed: December 27, 2006
    Date of Patent: March 24, 2015
    Assignee: Sekisui Medical Co., Ltd.
    Inventors: Mitsuaki Yamamoto, Tadaaki Yoshida
  • Patent number: 8940549
    Abstract: The present invention aims to provide an assay reagent and an assay for accurately measuring KL-6, in particular, an assay reagent and an assay for accurately measuring KL-6 in samples containing a rheumatoid factor and/or a nonspecific substance other than the rheumatoid factor. KL-6 in samples that contain a rheumatoid factor and/or a nonspecific substance other than the rheumatoid factor can be accurately measured using an immunoassay reagent comprising a solution at a pH of 4.0 to 5.5 containing a rheumatoid factor interference inhibitor and a solution of an insoluble carrier on which anti-KL-6 antibodies are immobilized.
    Type: Grant
    Filed: June 30, 2010
    Date of Patent: January 27, 2015
    Assignees: Sekisui Medical Co., Ltd., Eidia Co., Ltd.
    Inventors: Junichi Kondou, Mitsuaki Yamamoto, Chie Kaneko
  • Patent number: 8871518
    Abstract: A monoclonal antibody that does not show a crossreactivity with middle-molecular weight (MMW) adiponectin and specifically reacts with high-molecular weight (HMW) adiponectin alone is disclosed. The monoclonal antibody of the present invention can be produced by using HMW adiponectin as an antigen. According to the monoclonal antibody of the present invention, a convenient, high-accurate, and versatile reagent for analyzing HMW adiponectin can be provided.
    Type: Grant
    Filed: May 6, 2010
    Date of Patent: October 28, 2014
    Assignees: Otsuka Pharmaceutical Co., Ltd., LSI Medience Corporation
    Inventors: Suguru Akamatsu, Kiyonori Katsuragi, Hideaki Onishi, Midori Abe, Toru Hadama, Ayako Nishimura, Mio Ooguchi
  • Patent number: 8865425
    Abstract: The present invention relates to a reagent for the measurement of FDP comprising a carrier sensitized with at least two monoclonal antibodies selected from three monoclonal antibodies having different reactivity towards FDP. The present invention also relates to a reagent kit comprising the reagent and a method for measurement of FDP using the reagent or reagent kit.
    Type: Grant
    Filed: January 29, 2013
    Date of Patent: October 21, 2014
    Assignee: Sysmex Corporation
    Inventors: Katsushi Kobayashi, Masumi Murakami, Mayumi Sugimoto
  • Patent number: 8865424
    Abstract: The present invention relates to an anti-FDP monoclonal antibody selected from the first, second and third monoclonal antibodies having different reactivity towards FDP. The present invention also relates to a reagent and reagent kit for the measurement of FDP and a method for measurement of FDP using the anti-FDP monoclonal antibodies.
    Type: Grant
    Filed: January 28, 2013
    Date of Patent: October 21, 2014
    Assignee: Sysmex Corporation
    Inventors: Naoya Okitsu, Katsushi Kobayashi, Noriaki Nakajima, Masumi Murakami, Kouji Sakaguchi, Ayumi Asaeda, Mayumi Sugimoto
  • Patent number: 8759114
    Abstract: Peptides useful in determining the presence of autoantibodies in patients suffering from rheumatoid arthritis are disclosed.
    Type: Grant
    Filed: June 17, 2013
    Date of Patent: June 24, 2014
    Assignee: Stichting Voor de Technische Wetenschappen
    Inventors: Waltherus Jacobus Wilhelmus Van Venrooij, Jan Wouter Drijfhout, Martinus Adrianus Maria Van Boekel, Gerardus Jozef Maria Pruijn
  • Patent number: 8741600
    Abstract: A method of accurately separating immunoglobulin monomers by subjecting an immunoglobulin solution containing at least immunoglobulin monomers and immunoglobulin aggregates to cross-flow filtration using an ultrafiltration membrane, an ultrafiltration membrane module, and a cross-flow filtration apparatus. The method can separate immunoglobulin monomers by subjecting an immunoglobulin solution containing at least immunoglobulin monomers and immunoglobulin aggregates and having an immunoglobulin concentration of 1 to 150 g/L to cross-flow filtration using an ultrafiltration membrane having a molecular weight cut-off of 100,000 or more and less than 500,000 so that immunoglobulin monomers passes through the ultrafiltration membrane with a permeability of 80% or more while achieving a fractionation performance in which the permeability ratio of immunoglobulin dimers to immunoglobulin monomers that pass through the ultrafiltration membrane is 0.20 or less.
    Type: Grant
    Filed: June 18, 2008
    Date of Patent: June 3, 2014
    Assignee: Asahi Kasei Kabushiki Kaisha
    Inventors: Fumihiko Yamaguchi, Hitoshi Mizomoto, Shinya Hamasaki, Takena Mochizuki
  • Patent number: 8716033
    Abstract: The present invention generally relates to methods of functionalizing proteins, particularly antibodies, at oligosaccharide linkages, methods of humanizing antibodies by modifying glycosylation, as well as to novel antibodies linked to modified oligosaccharides. The invention further relates to kits that may be used to produce the antibodies of the invention.
    Type: Grant
    Filed: February 12, 2007
    Date of Patent: May 6, 2014
    Assignee: Life Technologies Corporation
    Inventors: Brian Agnew, Schuyler B. Corry, Kyle R. Gee
  • Patent number: 8691594
    Abstract: A method and apparatus for the manipulation of colloidal particulates and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled electrokinetic assembly of particles near surfaces relies on the combination of three functional elements: the AC electric field-induced assembly of planar aggregates; the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The present invention provides a set of fundamental operations enabling interactive control over the creation and placement of planar arrays of several types of particles and biomolecules and the manipulation of array shape and size. The present invention enables sample preparation and handling for diagnostic assays and biochemical analysis in an array format, and the functional integration of these operations.
    Type: Grant
    Filed: October 31, 2012
    Date of Patent: April 8, 2014
    Assignee: Bioarray Solutions, Ltd.
    Inventor: Michael Seul
  • Patent number: 8685662
    Abstract: The present invention pertains to a method for in vitro prognosticating and/or diagnosing cerebral cerebral malaria, wherein said method comprises a step of detecting non-erythroid spectrin or fragments thereof, and/or antibodies directed against non-erythroid spectrin, in a biological sample. Reagents and kits for performing this method are also disclosed.
    Type: Grant
    Filed: March 30, 2007
    Date of Patent: April 1, 2014
    Assignees: Institut Pasteur, Universite Pierre et Marie Curie (Paris 6)
    Inventors: Sylviane Pied, Vincent Guiyedi, Pierre-André Cazenave, Maryvonne Kombila, Youri Chanseaud
  • Patent number: 8580530
    Abstract: The invention relates to a method for detecting a plurality of antigenic molecules carried by erythrocytes and/or a plurality of anti-erythrocyte antibodies of an individual, comprising bringing a sample into contact with distinguishable beads, on which are attached a) antibodies specific for said antigens, or b) erythrocytes, erythrocyte membrane fragments or blood group antigens.
    Type: Grant
    Filed: June 6, 2008
    Date of Patent: November 12, 2013
    Assignee: Bio-Rad Innovations
    Inventors: Frederic Buffiere, Yves Raisin, Eliane Rivalin, Amparo Sanjuan
  • Patent number: 8580531
    Abstract: The invention relates to a method for detecting a plurality of antigenic molecules carried by erythrocytes and/or a plurality of anti-erythrocyte antibodies, said antigenic molecules carried by the erythrocytes consisting of antigenic molecules carried not only by the erythrocytes, but also by at least one other cell population, other than the blood group antigen molecules, said method comprising bringing a sample into contact with distinguishable beads, on which are attached a) antibodies specific for said antigens, or b) erythrocytes or erythrocyte membrane fragment.
    Type: Grant
    Filed: June 6, 2008
    Date of Patent: November 12, 2013
    Assignee: Bio-Rad Innovations
    Inventors: Frederic Buffiere, Yves Raisin, Eliane Rivalin, Amparo Sanjuan
  • Patent number: 8563329
    Abstract: Modified branched polymers are combined with bioactive agents which are one member of a binding pair for use in an assay.
    Type: Grant
    Filed: May 2, 2006
    Date of Patent: October 22, 2013
    Assignee: ANP Technologies, Inc.
    Inventors: Ray Yin, Dujie Qin, Jing Pan
  • Patent number: 8557604
    Abstract: A lateral flow, membrane-based assay device for detecting the presence or quantity of an analyte residing in a test sample is provided. The device utilizes phosphorescence to detect the signals generated by excited phosphorescent labels. The labels may have a long emission lifetime so that background interference from many sources, such as scattered light and autofluorescence, is practically eliminated during detection. In addition, the phosphorescent labels may be encapsulated within particles to shield the labels from quenchers, such as oxygen or water, which might disrupt the phosphorescent signal.
    Type: Grant
    Filed: December 14, 2007
    Date of Patent: October 15, 2013
    Assignee: Kimberly-Clark Worldwide, Inc.
    Inventor: Xuedong Song
  • Patent number: 8551786
    Abstract: Systems and methods for enhancing fluorescent detection of target molecules in a test sample are for use with an irradiating device. First fluorophores are provided for absorption of EMF radiation, and emission of a first signal. Second fluorophores are provided for partial absorption of the first signal, and emission of a second signal distinguishable from the first signal. The fluorophores are combined with the test sample, and secured to the target molecules and relative to one another. After the first fluorophores receive the EMF radiation from the irradiating device, the first signal is detected, together with the second spectral signal if the target molecules are present in the test sample.
    Type: Grant
    Filed: July 9, 2008
    Date of Patent: October 8, 2013
    Assignee: FIO Corporation
    Inventors: Warren Che Wor Chan, Travis Leon Jennings, Jesse M. Klostranec
  • Patent number: 8546148
    Abstract: A composition for use as an assay reagent comprises a solid support comprising a member of a signal producing system and a coating of a synthetic copolymer. The synthetic copolymer comprises a first polymerized monomer comprising a pendant moiety comprising a reactive functionality or a derivative of a reactive functionality and a second polymerized monomer comprising a pendant moiety comprising at least 1 carbon atoms and at least 2 heteroatoms. In some embodiments the copolymer comprises a polyethylenic backbone comprising the pendant moiety comprising a reactive functionality or a derivative of a reactive functionality and the pendant moiety comprising at least 1 carbon atom and at least 2 heteroatoms.
    Type: Grant
    Filed: April 9, 2012
    Date of Patent: October 1, 2013
    Assignee: Siemens Healthcare Diagnostics Inc
    Inventors: Bhalchandra Lele, Pratap Singh, Raphael Bartz, Jason A. Kellogg
  • Patent number: 8524452
    Abstract: Disclosed is an agent for inhibiting decrease in measured values in immunoassays, which may reduce the influences by interfering substances in a test sample so as to promote the accuracy of the immunoassays, as well as an immunoassay and a reagent for immunoassays using the same, with which the decrease in the measured values due to the interfering substances is reduced. The agent for inhibiting decrease in measured values in immunoassays, caused by interfering substances, is an ionic surfactant having a molecular weight of 1000 to 100,000, the agent being a polymer in which a hydrophobic cyclic monomer(s) having an ionic functional group(s) is(are) polymerized.
    Type: Grant
    Filed: February 25, 2005
    Date of Patent: September 3, 2013
    Assignee: Denka Seiken Co., Ltd.
    Inventors: Yasunori Minakawa, Michie Saito, Hiroshi Matsui
  • Patent number: 8518713
    Abstract: Generally, conjugate systems, self-illuminating quantum dot conjugates, methods of detecting a target in a host, methods of treating a disease in a host, and the like, are described herein.
    Type: Grant
    Filed: June 13, 2012
    Date of Patent: August 27, 2013
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Jianghong Rao, Min-kyung So, Chenjie Xu, Andreas M. Loening, Sanjiv S. Gambhir
  • Patent number: 8513024
    Abstract: Provided is a detection method for a target substance capable of enhancing detection sensitivity and quantitative property of a magnetic biosensor, while keeping monodispersity and dispersion stability of magnetic markers, including the steps of: reacting the target substance in a sample solution with a first target substance trapping member immobilized on a sensing element and with a second target substance trapping member immobilized on a gel particle to hold the gel particle on the sensing element; adjusting a magnetic marker precursor including the gel particle and a magnetic material precursor existing in the gel particle by bringing the magnetic material precursor into contact with the gel particle; synthesizing a magnetic material from the magnetic material precursor held on the gel particle, thereby adjusting the magnetic markers; and detecting the magnetic markers with the sensing element.
    Type: Grant
    Filed: February 4, 2009
    Date of Patent: August 20, 2013
    Assignee: Canon Kabushiki Kaisha
    Inventor: Kazumichi Nakahama
  • Patent number: 8497137
    Abstract: Capture particles for harvesting analytes from solution and methods for using them are described. The capture particles are made up of a polymeric matrix having pore size that allows for the analytes to enter the capture particles. The pore size of the capture particles are changeable upon application of a stimulus to the particles, allowing the pore size of the particles to be changed so that analytes of interest remain sequestered inside the particles. The polymeric matrix of the capture particles are made of co-polymeric materials having a structural monomer and an affinity monomer, the affinity monomer having properties that attract the analyte to the capture particle. The capture particles may be used to isolate and identify analytes present in a mixture. They may also be used to protect analytes which are typically subject to degradation upon harvesting and to concentrate low an analyte in low abundance in a fluid.
    Type: Grant
    Filed: August 19, 2008
    Date of Patent: July 30, 2013
    Inventors: Alessandra Luchini, Lance Liotta, Emanuel Petricoin, Barney Bishop, Caterina Longo, Virginia Espina, Alexis Patanarut
  • Patent number: 8481332
    Abstract: A liposomal composition, preferably a vaccine, comprising liposomes formed of liposome forming compounds, containing coentrapped polysaccharide antigen and T-cell dependent protein carrier, such as tetanus toxoid or diphtheria toxin modified to render it non-toxic. The invention is of use in the production of vaccines against Haemophilus influenzae, Streptococcus pneumoniae or Neisseria meningitidis.
    Type: Grant
    Filed: January 24, 2011
    Date of Patent: July 9, 2013
    Assignee: Stichting Voor de Technische Wetenshappen
    Inventors: Waltherus Jacobus Wilhelmus Van Venrooij, Jan Wouter Drijfhout, Martinus Adrianus Maria Van Boekel, Gerardus Jozef Maria Pruijn
  • Patent number: 8481326
    Abstract: A microfluidic device and method for measuring a level of cholesterol therewith are provided. The cholesterol measurement apparatus includes a microfluidic device including a plurality of chambers and at least one channel through which the plurality of chambers are interconnected. The plurality of chambers include a reaction chamber which contains a capture binder, a buffer chamber which contains an elution buffer and is connected to the reaction chamber, and at least one detection chamber which contains a cholesterol measurement reagent and is connected to the reaction chamber.
    Type: Grant
    Filed: July 8, 2011
    Date of Patent: July 9, 2013
    Assignee: Samsung Electronics Co., Ltd.
    Inventor: In Wook Kim
  • Patent number: 8456158
    Abstract: A magnetic binding substance, which is a first binding substance that specifically binds with a target substance, having magnet enveloping dielectric particles, which have magnetic particles enveloped therein and surfaces modified with functional groups that exhibit polarity within a liquid sample, attached thereto, and a labeling binding substance, which is a second binding substance that specifically binds with the target substance having photoresponsive labels attached thereto, are mixed with the liquid sample such that binding reactions occur. A magnetic field is generated within a sample cell, to draw the magnetic binding substance to a local region. Excitation light is irradiated only onto a predetermined region including the local region while the magnetic binding substance is drawn to the local region, causing the photoresponsive labels present therein to generate optical signals. The optical signals are detected.
    Type: Grant
    Filed: July 28, 2010
    Date of Patent: June 4, 2013
    Assignee: FUJIFILM Corporation
    Inventor: Hisashi Ohtsuka
  • Patent number: 8431415
    Abstract: The present invention provides a reagent for an immunoassay comprising insoluble carrier particles which can give the values to be determined with high accuracy and reliability, and can be stored for a long time; an immunoassay using the reagent; and a method for keeping the reagent stable.
    Type: Grant
    Filed: July 27, 2009
    Date of Patent: April 30, 2013
    Assignee: TFB, Inc.
    Inventor: Kayoko Shigenobu
  • Patent number: 8394647
    Abstract: Methods and reagents are disclosed for reducing an amount of non-covalently bound polysaccharide on a support. The method comprises contacting a support comprising both covalently bound polysaccharide and non-covalently bound polysaccharide with an aqueous solution comprising an amount of a chaotropic agent effective to remove non-covalently bound polysaccharide from the support.
    Type: Grant
    Filed: February 17, 2011
    Date of Patent: March 12, 2013
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventors: Bhalchandra Lele, Pratap Singh, Asoke Chatterjee
  • Patent number: 8383424
    Abstract: Methods and reagents are disclosed for determining the presence and/or amount of cyclosporin A in a medium suspected of containing cyclosporin A. In the method a combination is provided in a medium. The combination comprises (i) the sample, (ii) a first member of a signal producing system (sps) associated with a first support wherein the first sps member is capable of activating a second member of the sps and wherein the first support is associated with a first member of a specific binding pair, and (iii) the second sps member associated with a second support wherein the second sps member is activatable by the first sps member. The second support comprises either (I) cyclosporin C or cyclosporin A and the combination further comprises a conjugate of an antibody for cyclosporin A and a second member of the specific binding pair or (II) antibody for cyclosporin A and the combination further comprises a conjugate of cyclosporin A and a second member of the specific binding pair.
    Type: Grant
    Filed: April 27, 2011
    Date of Patent: February 26, 2013
    Assignee: Siemens Healthcare Diagnostics
    Inventors: Yi Feng Zheng, Deb K. Vickery, Susan C. Swann
  • Patent number: 8354235
    Abstract: The invention concerns a method for increasing the dynamic measuring range of especially immunological test elements in particular immunological chromatography test strips that can be evaluated optically that are based on specific binding reactions. The invention enables the dynamic measuring range of test elements based on specific binding reagents, especially of immunological test elements to be shifted towards higher analyte concentrations without impairing the lower detection limit.
    Type: Grant
    Filed: November 7, 2006
    Date of Patent: January 15, 2013
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Ludger Bodenbach, Juergen Schaeffler, Juergen Spinke
  • Patent number: 8318492
    Abstract: The present invention relates in one aspect to a method for determining the cell culture history of a cell unit labelled with more than one type of tag comprising the steps of: (a) measuring one or more parameters of each tag that is used to label the cell unit; (b) identifying each tag in the cell unit; and (c) correlating the identity of each tag to the identity of the cell unit and/or the specific cell culture conditions to which the cell unit has been exposed.
    Type: Grant
    Filed: February 25, 2008
    Date of Patent: November 27, 2012
    Assignee: Plasticell Limited
    Inventors: Yen Choo, Fraser Hornby, John Girdlestone
  • Patent number: 8309368
    Abstract: A method and apparatus for the manipulation of colloidal particulates and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled electrokinetic assembly of particles near surfaces relics on the combination of three functional elements: the AC electric field-induced assembly of planar aggregates; the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The present invention provides a set of fundamental operations enabling interactive control over the creation and placement of planar arrays of several types of particles and biomolecules and manipulation of array shape and size. The present invention enables sample preparation and handling for diagnostic assays and biochemical analysis in an array format, and the functional integration of these operations.
    Type: Grant
    Filed: June 11, 2007
    Date of Patent: November 13, 2012
    Assignee: BioArray Solutions, Ltd.
    Inventor: Michael Seul
  • Patent number: 8263417
    Abstract: Generally, conjugate systems, self-illuminating quantum dot conjugates, methods of detecting a target in a host, methods of treating a disease in a host, and the like, are described herein.
    Type: Grant
    Filed: January 4, 2007
    Date of Patent: September 11, 2012
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Jianghong Rao, Min-kyung So, Chenjie Xu, Andreas M. Loening, Sanjiv S. Gambhir
  • Patent number: 8252569
    Abstract: A microfluidic device for the concentration and lysis of cells or viruses and a method of concentrating and lysing cells or viruses using the microfluidic device include: magnetic beads, a reaction chamber in which the magnetic beads are accommodated and a laser source. The reaction chamber includes a plurality of electrodes which cross each other and are separated by a dielectric to generate an electric field and a vibrating part agitating the magnetic beads in the chamber. The laser source radiates a laser onto the magnetic beads in the reaction chamber.
    Type: Grant
    Filed: February 25, 2011
    Date of Patent: August 28, 2012
    Assignee: Samsung Electronics Co., Ltd.
    Inventors: Yoon-kyoung Cho, Jeong-gun Lee, Sung-young Jeong
  • Patent number: 8227263
    Abstract: A process for producing polymer particles having an antigen or an antibody introduced into the surface thereof, by carrying out miniemulsion polymerization using a monomer, a radical polymerization initiator, an emulsifier, and a hydrophobe in the presence of the antigen or antibody to thereby produce the polymer particles, is disclosed. According to the process, it is possible to provide a reagent for immunological analysis having an excellent detection sensitivity and capable of avoiding a nonspecific reaction which occurs in conventional methods.
    Type: Grant
    Filed: October 16, 2007
    Date of Patent: July 24, 2012
    Assignees: Mitsubishi Chemical Medience Corporation, National University Corporation Chiba University
    Inventors: Tatsuo Taniguchi, Akihiro Mizuno, Tokio Sawai, Hiroyuki Tsubota
  • Patent number: 8182746
    Abstract: An electrophoresis apparatus is generally disclosed for sequentially analyzing a single sample or multiple samples having one or more analytes in high or low concentrations. The apparatus comprises a relatively large-bore transport capillary which intersects with a plurality of small-bore separation capillaries and includes a valve system. Analyte concentrators, having antibody-specific (or related affinity) chemistries, are stationed at the respective intersections of the transport capillary and separation capillaries to bind one or more analytes of interest. The apparatus allows the performance of two or more dimensions for the optimal separation of analytes. The apparatus may also include a plurality of valves surrounding each of the analyte concentrators to localize each of the concentrators to improve the binding of one or more analytes of interest.
    Type: Grant
    Filed: October 31, 2007
    Date of Patent: May 22, 2012
    Assignee: Princeton Biochemicals, Inc.
    Inventor: Norberto A. Guzman
  • Patent number: 8153442
    Abstract: Methods and reagents are disclosed for conducting assays. Embodiments of the present methods and reagents are concerned with a solid support such as, for example, a particle. The support includes a chemiluminescent composition that includes a metal chelate. The present inventors observed that, when such support such as, e.g., particles, were employed in assays for the determination of an analyte, stability of signal output by the chemiluminescent composition associated with the particle was unacceptably reduced as compared to particles including other chemiluminescent compositions. In accordance with embodiments of the present invention, the stability of signal output from such particles is enhanced by including in a medium that contains the particles a sufficient amount of one or more stabilizing agents, which may be a chelating agent and/or a metal chelate such as, for example, the metal chelate that is associated with the particle.
    Type: Grant
    Filed: October 21, 2009
    Date of Patent: April 10, 2012
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventors: Alan R. Craig, Zhu Teng, Carsten Schelp, Jason Snyder, Christine Moran
  • Patent number: 8137985
    Abstract: A flow-through assay for detecting the quantity of an analyte residing in a test sample is provided. The flow-through assay contains a porous membrane that is in fluid communication with probe conjugates that contain a specific binding member and a detectable probe. The porous membrane also defines a detection zone and a calibration zone. The calibration zone contains a polyelectrolyte substantially non-diffusively immobilized on the porous membrane. The polyelectrolyte is capable of generating a detectable calibration signal that can be readily compared (visually, quantitatively, and the like) to a detection signal to determine the amount of analyte in the test sample.
    Type: Grant
    Filed: November 12, 2009
    Date of Patent: March 20, 2012
    Assignee: Kimberly-Clark Worldwide, Inc.
    Inventors: Xuedong Song, Ning Wei, Curtis N. Sayre
  • Patent number: 8110407
    Abstract: Disclosed is a fluorescent semiconductor microparticle assembly comprising at least three kinds of fluorescent semiconductor microparticles with an average particle size of from 1 to 10 nm, having the same chemical composition, a different average particle size and a different emission maximum wavelength in the emission spectra, wherein a standard deviation of emission intensity in each of the at least three kinds of fluorescent semiconductor microparticles is not more than 15%.
    Type: Grant
    Filed: August 22, 2007
    Date of Patent: February 7, 2012
    Assignee: Konica Minolta Medical & Graphic, Inc.
    Inventors: Kazuya Tsukada, Hisatake Okada, Hideki Hoshino
  • Patent number: 8101429
    Abstract: This invention is directed to a lateral flow assay for detecting the presence of an analyte in a liquid test sample. The lateral flow assay represents an improvement in the ability to accurately and with high fidelity to detect the presence or absence of a target analyte in a liquid sample, in part, by encompassing a reference region of immobilized, non-diffusible analyte that allows for detection of any factors that interfere with the interaction and binding of the analyte to the labeled capture reagent. Any influences on the interaction and binding of the analyte that is free in solution in the liquid test sample to its complementary labeled reagent will be encountered in parallel in the binding between the immobilized analyte in reference region to the labeled reagent as it diffuses through the reference region. In one embodiment, the lateral flow assay of the invention is a urine-based human Chorionic Gonadotropin (hCG) assay.
    Type: Grant
    Filed: June 3, 2004
    Date of Patent: January 24, 2012
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventors: Gary H. Krauth, David J. Ledden
  • Patent number: 8030092
    Abstract: An electrophoresis apparatus is generally disclosed for sequentially analyzing a single sample or multiple samples having one or more analytes in high or low concentrations. The apparatus comprises a relatively large-bore transport capillary which intersects with a plurality of small-bore separation capillaries and includes a valve system. Analyte concentrators, having antibody-specific (or related affinity) chemistries, are stationed at the respective intersections of the transport capillary and separation capillaries to bind one or more analytes of interest. The apparatus allows the performance of two or more dimensions for the optimal separation of analytes. The apparatus may also include a plurality of valves surrounding each of the analyte concentrators to localize each of the concentrators to improve the binding of one or more analytes of interest.
    Type: Grant
    Filed: November 17, 2006
    Date of Patent: October 4, 2011
    Assignee: Princeton Biochemicals, Inc.
    Inventor: Norberto A. Guzman
  • Patent number: 8026107
    Abstract: Provided are an agglutination-inhibition assay and a reagent for agglutination-inhibition assay, which can be used for measuring a ligand in a sample at high sensitivity in a wide range from the low-concentration range to the high-concentration range and have good reproducibility of measurement. Specifically, provided are an agglutination-inhibition assay and a reagent for agglutination-inhibition assay, in which used are an insoluble carrier particle carrying a ligand, a specific receptor in the free-form and an insoluble carrier particle carrying a specific receptor which binds to a different site on the ligand than the receptor in the free-form.
    Type: Grant
    Filed: July 23, 2007
    Date of Patent: September 27, 2011
    Assignee: Sekisui Medical Co., Ltd.
    Inventors: Tadaaki Yoshida, Hiroshi Takahashi
  • Patent number: 7968347
    Abstract: An example method for detecting an analyte in a sample of a bodily fluid comprises the steps of exposing the bodily fluid sample to electromagnetic energy to cause a thermoelastic expansion in the analyte, and detecting a photoacoustic signal in the sample that results from the thermoelastic expansion.
    Type: Grant
    Filed: July 11, 2007
    Date of Patent: June 28, 2011
    Assignee: The Curators of the University of Missouri
    Inventors: John A. Viator, Paul S. Dale, Ryan M. Weight, Peter Sutovsky
  • Patent number: 7897328
    Abstract: The present invention provides compositions and methods for treating or preventing antibody mediated graft rejection and blood typing.
    Type: Grant
    Filed: March 23, 2007
    Date of Patent: March 1, 2011
    Assignee: AbSorber AB
    Inventors: Jan Holgersson, Jining Liu, Linda Lindberg, Per Grufman
  • Patent number: 7892762
    Abstract: A method for aiding in differentiating irritable bowel syndrome from inflammatory bowel disease by determining the level of total endogenous human lactoferrin in clinical specimens, such as feces, mucus and bile, wherein an elevated level of lactoferrin substantially precludes diagnoses of IBS and other noninflammatory etiologies, and a kit usable in such method are provided. Further provided is a method for quantitating the level of total endogenous human lactoferrin in clinical specimens, such as feces, mucus and bile, to monitor gastrointestinal inflammation in persons having inflammatory bowel disease.
    Type: Grant
    Filed: June 17, 2009
    Date of Patent: February 22, 2011
    Assignee: Techlab, Inc.
    Inventors: James Hunter Boone, David Maxwell Lyerly, Tracy Dale Wilkins, Richard Littleton Guerrant
  • Patent number: 7893177
    Abstract: The invention provides nanoparticles and nanoparticle conjugates comprising one or more redox-active species, methods of making nanoparticles and nanoparticle conjugates, and methods for using nanoparticles and nanoparticle conjugates, for example, as diagnostic agents for the detection of various analytes.
    Type: Grant
    Filed: October 31, 2007
    Date of Patent: February 22, 2011
    Assignee: Iowa State University Research Foundation, Inc.
    Inventors: Marc D. Porter, Jennifer H. Granger
  • Patent number: 7888133
    Abstract: The invention relates to method of detecting autoantibodies from patients suffering from rheumatoid arthritis. To this end, according to the invention, at least two peptide units are used of which at least one peptide unit comprises a part not derived from (pro)filaggrin, fibrin, fibrinogen, vimentin, cytokeratin 1 and cytokeratin 9, and which peptide unit comprises the motif XG, and a peptide unit comprising the motif XnonG, wherein X is a citrulline or an analogue thereof, and nonG is an amino acid other than glycine.
    Type: Grant
    Filed: December 11, 2002
    Date of Patent: February 15, 2011
    Assignee: Stichting Voor De Technische Wetenschappen
    Inventors: Waltherus Jacobus Wilhelmus Van Venrooij, Jan Wouter Drijfhout, Martinus Adrianus Maria Van Boekel, Gerardus Jozef Maria Pruijn
  • Patent number: RE43979
    Abstract: The invention relates to a method for detecting specific target-cells in a simple and time saving way, using paramagnetic particles, antibodies recognizing the Fc portions of target-cell associating antibodies and target-cell associating antibodies directed to specific antigen determinants in the target-cell membranes. Incubation of the cell suspension with a mild detergent and/or second set of antibodies or antibody fragments, prelabeled or not with fluorescent agents, metallocolloids, radioisotopes, biotincomplexes or certain enzymes allowing visualization, with dramatically increase the specificity of the method. The method can further be used for isolation of the target-cells by magnetic field application and kit for performing the method according to the invention is described.
    Type: Grant
    Filed: February 5, 2003
    Date of Patent: February 5, 2013
    Assignee: Abbott Laboratories
    Inventors: Oystein Fodstad, Gunnar Kvalheim