Carrier Is Synthetic Resin Patents (Class 436/531)
  • Patent number: 10301667
    Abstract: Described herein are fluid-manipulation-based devices. Fluid manipulations as described herein can be configured to perform assays on biological samples. In an embodiment, the device includes a reaction chamber, which can includes an integrated sample isolation module, a cell lysis module, a biological target purification module, and an assay mixing module, which can include a microbead with a capture molecule coupled thereto and a nanoparticle having a probe molecule coupled thereto via a label, which can be a spectroscopic label. In an embodiment, the capture and probe molecules can be configured to be coupled together via a biological target to form a biological molecule bead complex. Devices and methods as described herein can manipulate and analyze nanoliter volumes of fluid, microliter volumes of fluid, milliliter volumes of fluid, or greater. Embodiments of the present disclosure can enable random biological assays and rapid, simultaneous analysis of multiple biological samples.
    Type: Grant
    Filed: November 18, 2016
    Date of Patent: May 28, 2019
    Assignee: University of Florida Research Foundation, Inc.
    Inventor: Yunwei Charles Cao
  • Patent number: 10188999
    Abstract: Globular microstructures comprising a liquid core and a solid shell that envelops the core comprising polymeric micro- or nanofibers, preferably obtained by electrospinning, comprising a hydrophobic polymer or a mixture of hydrophobic polymer with polymers derived from cellulose or with polyacrylates; the microstructures may have a further coating of nanoparticles or polymeric coating. The microstructures have applications similar to those of “liquid marbles” with improved mechanical properties.
    Type: Grant
    Filed: December 16, 2014
    Date of Patent: January 29, 2019
    Assignee: Fondazione Istituto Italiano di Technologia
    Inventors: Elisa Mele, Ilker S. Bayer, Pier Paolo Pompa, Gabriele Maiorano, Athanasia Athanasiou, Roberto Cingolani
  • Patent number: 9726596
    Abstract: A method of determining the relative concentrations of enantiomeric forms of a compound in an enantiomeric mixture includes combining the enantiomeric mixture with carbon nanotubes or graphene to form a carbon-enantiomer mixture, exposing the mixture to a monochromatic polarized light, and analyzing reflected polarized light from the mixture using a differential analyzer.
    Type: Grant
    Filed: November 12, 2014
    Date of Patent: August 8, 2017
    Assignee: UNIVERSITY OF CALCUTTA
    Inventors: Anjan Kr. Dasgupta, Tamoghna Bhattacharyya, Sarita Roy
  • Patent number: 9499679
    Abstract: The present invention provides aqueous binder compositions comprising one or more polyol and a multistage copolymer, preferably, an all acrylic and allylic or all acrylic copolymer, having as the outermost stage a polymerized acid functional aqueous solution polymer and as the one or more remaining stage(s) a vinyl emulsion polymer, wherein the emulsion polymer stage(s) in the multistage copolymer comprises 5 wt. % or less of hydrophilic monomer, based on the total weight of monomers used to make the emulsion polymer stage(s). The multistage copolymer enables incorporation of a much higher amount of hydrophilic monomer into a polymeric binder without the attendant handling or viscosity problems. Also provided are methods of using the aqueous binder compositions comprising pultruding two or more non-woven fibers or a fiber roving with the aqueous binder composition, and drying, Articles comprising the bound fiber(s) are provided.
    Type: Grant
    Filed: September 24, 2013
    Date of Patent: November 22, 2016
    Assignee: Rohm and Haas Company
    Inventors: Stephen A. Crescimanno, Michael D. Kelly
  • Patent number: 9481720
    Abstract: The present invention relates to a polypeptide carrying a human BNP(I-32) epitope according to Formula (I): a1-R1-X1-FGRKMDR-X2-R2-a2 as well as ligands specific of the FGRKMDR epitope.
    Type: Grant
    Filed: August 1, 2008
    Date of Patent: November 1, 2016
    Assignee: BIO-RAD INNOVATIONS
    Inventors: Francois Rieunier, Isabelle Giuliani, Sylvie Villard-Saussine
  • Patent number: 9241977
    Abstract: Techniques and devices for removing (filtering out) unwanted/inhibitory components (for example, products, byproducts and/or cell output such as inhibitory catabolic proteins) from a composition (for example, autologous fluid or serum) containing such unwanted/inhibitory components. The devices include at least one construct designed to contain a composition (for example, autologous fluid or serum) containing inhibitory/unwanted components (products, byproducts and/or output of cells such as inhibitory catabolic proteins). The construct is provided with interior walls including specific protein capturing means designed to remove the inhibitory/unwanted component(s) (for example, specific protein(s)) from the composition. Proteins targeted for capture include, but are not limited to, catabolic cytokines such as IL-1?, IL-?, IL-6, TNF?, IFN? and MMPS as these proteins inhibit the healing process.
    Type: Grant
    Filed: February 25, 2014
    Date of Patent: January 26, 2016
    Assignee: Arthrex, Inc.
    Inventors: Christopher M. Bare, Brandon L. Roller, Melissa S. Tucker
  • Patent number: 9034657
    Abstract: A two step lateral flow assay method for identifying IgE antibodies in a sample comprises applying a sample to a sample port of a device, wherein the device is adapted to deliver the sample to a lateral flow matrix having a plurality of IgE antigen species immobilized at respective positions at a first location; allowing the sample to travel along the lateral flow matrix through the immobilized plurality of IgE antigen species to a second location downstream of the first location; and, after a predetermined period of time, applying liquid buffer to the lateral flow matrix to mobilize labeled reagent which is adapted to bind anti-IgE antibody and which is dried on the lateral flow matrix at a location upstream of the sample port delivery of the sample to the lateral flow matrix, and allowing labeled reagent mobilized by the liquid buffer to travel along the lateral flow matrix through the immobilized plurality of IgE antigen species and bind with any IgE antibody bound to the immobilized IgE antigen species, a
    Type: Grant
    Filed: December 8, 2010
    Date of Patent: May 19, 2015
    Assignee: Phadia AB
    Inventors: Gerd Rundström, Per Matsson, Paul Christopher
  • Patent number: 9012240
    Abstract: An immunoassay device incorporating porous polymeric capture nanoparticles within either the sample collection vessel or pre-impregnated into a porous substratum within fluid flow path of the analytical device is presented. This incorporation of capture particles within the immunoassay device improves sensitivity while removing the requirement for pre-processing of samples prior to loading the immunoassay device. A preferred embodiment is coreshell bait containing capture nanoparticles which perform three functions in one step, in solution: a) molecular size sieving, b) target analyte sequestration and concentration, and c) protection from degradation. The polymeric matrix of the capture particles may be made of co-polymeric materials having a structural monomer and an affinity monomer, the affinity monomer having properties that attract the analyte to the capture particle.
    Type: Grant
    Filed: August 26, 2009
    Date of Patent: April 21, 2015
    Assignee: George Mason Research Foundation, Inc.
    Inventors: Lance A. Liotta, Alessandra Luchini, Emanuel F. Petricoin, Virginia Espina
  • Patent number: 9012239
    Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).
    Type: Grant
    Filed: May 28, 2007
    Date of Patent: April 21, 2015
    Assignee: Fluidigm Canada Inc.
    Inventors: Mitchell A. Winnik, Mark Nitz, Vladimir Baranov, Xudong Lou
  • Patent number: 9005996
    Abstract: The current invention is a capture-particle comprising: a) a molecular sieve portion; and b) an analyte binding portion; wherein the molecular sieve portion, analyte binding portion or both further comprise a cross-linked region having modified porosity. Capture particles wherein the molecular sieve portion, analyte binding portion or both comprise pore dimensions sufficient to exclude molecules larger than about 60 kDa. These particles are useful in purification and diagnostic methods. Kits comprising the capture particles are also described.
    Type: Grant
    Filed: September 27, 2006
    Date of Patent: April 14, 2015
    Assignee: George Mason Research Foundation
    Inventors: Lance Liotta, Emanuel Petricoin, David Geho
  • Patent number: 8987005
    Abstract: [PROBLEMS] To provide a reagent for measuring agglutination by using a reaction accelerator, which causes no spontaneous agglutination of receptor-sensitized carrier particles in the coexistence of these carrier particles, and a measurement method. [MEANS FOR SOLVING PROBLEMS] A reagent for measuring agglutination by using a specific amine compound, whereby aggregation based on a specific reaction can be accelerated without causing spontaneous agglutination of carrier particles, and measurement method.
    Type: Grant
    Filed: December 27, 2006
    Date of Patent: March 24, 2015
    Assignee: Sekisui Medical Co., Ltd.
    Inventors: Mitsuaki Yamamoto, Tadaaki Yoshida
  • Patent number: 8940549
    Abstract: The present invention aims to provide an assay reagent and an assay for accurately measuring KL-6, in particular, an assay reagent and an assay for accurately measuring KL-6 in samples containing a rheumatoid factor and/or a nonspecific substance other than the rheumatoid factor. KL-6 in samples that contain a rheumatoid factor and/or a nonspecific substance other than the rheumatoid factor can be accurately measured using an immunoassay reagent comprising a solution at a pH of 4.0 to 5.5 containing a rheumatoid factor interference inhibitor and a solution of an insoluble carrier on which anti-KL-6 antibodies are immobilized.
    Type: Grant
    Filed: June 30, 2010
    Date of Patent: January 27, 2015
    Assignees: Sekisui Medical Co., Ltd., Eidia Co., Ltd.
    Inventors: Junichi Kondou, Mitsuaki Yamamoto, Chie Kaneko
  • Patent number: 8900881
    Abstract: The present invention relates to a quantitative assay device and a method for the determination of an analyte, based on a test strip, which contains a porous test membrane allowing for capillary flow of the analyte and complexes of the analyte, a porous upstream membrane in fluid connection with the test membrane and a porous downstream membrane in fluid connection with the test membrane, wherein the test membrane contains two bands having deposited on there high and low concentrations of different calibrator agents and a test band capable of reacting with conjugated analyte complexes giving rise to a measurable signal.
    Type: Grant
    Filed: September 12, 2011
    Date of Patent: December 2, 2014
    Inventor: Jin Po Lee
  • Patent number: 8835144
    Abstract: Dual-functional nonfouling surfaces and materials, methods for making dual-functional nonfouling surfaces and materials, and devices that include dual-functional nonfouling surfaces and materials. The dual-functional surfaces are nonfouling surfaces that resist non-specific protein adsorption and cell adhesion. The dual-functional surfaces and materials include covalently coupled biomolecules (e.g., target binding partners) that impart specific biological activity thereto. The surfaces and materials are useful in medical diagnostics, biomaterials and bioprocessing, tissue engineering, and drug delivery.
    Type: Grant
    Filed: June 29, 2009
    Date of Patent: September 16, 2014
    Assignee: University of Washington
    Inventors: Shaoyi Jiang, Zheng Zhang, Shengfu Chen, Hana Vaisocherova
  • Patent number: 8809071
    Abstract: The invention provides a method for immobilization of biological molecules such as nucleic acids, peptides and proteins onto the surface of a glass or plastic solid support.
    Type: Grant
    Filed: November 29, 2002
    Date of Patent: August 19, 2014
    Inventor: Marcella Chiari
  • Patent number: 8802447
    Abstract: The present invention provides materials, devices, and methods related to determination of an analyte. In some embodiments, an analyte may be determined by monitoring, for example, a change in an optical signal of a luminescent material (e.g., particle) upon exposure to an analyte. The present invention may be particularly advantageous in that some embodiments may comprise an emissive species useful as an internal reference standard. Methods of the invention may also be useful in the quantitative determination of an analyte. In some cases, the present invention may allow for selective determination of an analyte.
    Type: Grant
    Filed: November 10, 2006
    Date of Patent: August 12, 2014
    Assignee: Massachusetts Institute of Technology
    Inventors: Timothy M. Swager, Jessica H. Liao
  • Patent number: 8778699
    Abstract: A method for determining the amount of NT-proBNP in blood samples from animals. The method includes detecting degradation products of NT-proBNP by various methods, including using antibodies, kits and device.
    Type: Grant
    Filed: August 4, 2011
    Date of Patent: July 15, 2014
    Assignee: Idexx Laboratories, Inc.
    Inventors: Mahalakshmi Yerramilli, Michael Atkinson, Murthy V. S. N. Yerramilli
  • Patent number: 8778613
    Abstract: A resin which is prepared by polymerizing a monomer component incorporating a hydrophilic spacer, and a ligand-immobilized solid phase carrier obtained by immobilizing a ligand to the resin, are capable of reducing the non-specific adsorption of substances, other than the target molecule for the ligand, which mingle in the sample, to the resin and/or the ligand. Therefore, target molecule search, identification and the like with less noise are enabled.
    Type: Grant
    Filed: October 15, 2004
    Date of Patent: July 15, 2014
    Assignee: Reverse Proteomics Research Institute Co., Ltd.
    Inventors: Akito Tanaka, Takaaki Shiyama, Akira Yamazaki
  • Patent number: 8765469
    Abstract: A method for preparing lymphocytes characterized in that the method comprises the step of carrying out expansion in the presence of (a) fibronectin, a fragment thereof or a mixture thereof, (b) a CD3 ligand, and (c) a CD28 ligand.
    Type: Grant
    Filed: August 10, 2006
    Date of Patent: July 1, 2014
    Assignee: Takara Bio Inc.
    Inventors: Takahiro Marui, Kinuko Nagamine, Nobuko Muraki, Akiko Kato, Tatsuji Enoki, Hiroaki Sagawa, Ikunoshin Kato
  • Patent number: 8765488
    Abstract: Sensing compositions, sensing element, sensing systems and sensing devices for the detection and/or quantitation of one or more analytes, Compositions comprising carbon nanotubes in which the carbon nanotubes retain their ability to luminesce and in which that luminescence is rendered selectively sensitive to the presence of an analyte. Compositions comprising individually dispersed carbon nanotubes, which are electronically isolated from other carbon nanotubes, yet which are associated with chemical selective species, such as polymers, particularly biological polymers, for example proteins, which can interact selectively with, or more specifically selectivity bind to, an analyte of interest. Chemically selective species bind, preferably non-covalently, to the carbon nanotube and function to provide for analyte selectivity. Chemically selective species include polymers to which one or more chemically selective groups are covalently attached.
    Type: Grant
    Filed: July 22, 2005
    Date of Patent: July 1, 2014
    Assignee: The Board of Trustees of the University of Illinois
    Inventors: Michael S. Strano, Seunghyun Baik, Paul Barone
  • Patent number: 8759114
    Abstract: Peptides useful in determining the presence of autoantibodies in patients suffering from rheumatoid arthritis are disclosed.
    Type: Grant
    Filed: June 17, 2013
    Date of Patent: June 24, 2014
    Assignee: Stichting Voor de Technische Wetenschappen
    Inventors: Waltherus Jacobus Wilhelmus Van Venrooij, Jan Wouter Drijfhout, Martinus Adrianus Maria Van Boekel, Gerardus Jozef Maria Pruijn
  • Patent number: 8753872
    Abstract: A method and apparatus for assay of multiple analytes. The method uses a sensing element comprising a substrate upon which is arranged a multiplicity of recognition elements, such that each element is laid out in a predetermined pattern. Each pattern is unique in that it can give rise to a characteristic diffraction pattern in the assay. The patterns may or may not be interpenetrating on the substrate surface. The method of detecting multiple analytes includes contacting the medium of analytes with the patterned substrate, illuminating the substrate by a light source, and detecting any resultant diffraction image. The pattern of diffraction and the intensity of the diffracted signal provides information about the existence of specific analytes and their quantification.
    Type: Grant
    Filed: December 20, 2005
    Date of Patent: June 17, 2014
    Assignee: Axela Inc.
    Inventors: M. Cynthia Goh, Jane B. Goh, Richard Mcaloney, Richard Loo
  • Patent number: 8697111
    Abstract: An osteochondral plug includes a first scaffold and a second scaffold. The first scaffold may be a solid scaffold containing one or more pendant reactive functional groups. The second scaffold capable of reacting with the one or more pendant reactive functional groups of the first scaffold.
    Type: Grant
    Filed: May 12, 2010
    Date of Patent: April 15, 2014
    Assignee: Covidien LP
    Inventors: Arpan Desai, Timothy Sargeant, Atu Agawu, Joshua Stopek
  • Patent number: 8685662
    Abstract: The present invention pertains to a method for in vitro prognosticating and/or diagnosing cerebral cerebral malaria, wherein said method comprises a step of detecting non-erythroid spectrin or fragments thereof, and/or antibodies directed against non-erythroid spectrin, in a biological sample. Reagents and kits for performing this method are also disclosed.
    Type: Grant
    Filed: March 30, 2007
    Date of Patent: April 1, 2014
    Assignees: Institut Pasteur, Universite Pierre et Marie Curie (Paris 6)
    Inventors: Sylviane Pied, Vincent Guiyedi, Pierre-André Cazenave, Maryvonne Kombila, Youri Chanseaud
  • Patent number: 8652849
    Abstract: This invention relates to a composite material that comprises a support member that has a plurality of pores extending through the support member and, located in the pores of the support member, and filling the pores of the support member, a macroporous cross-linked gel. The invention also relates to a process for preparing the composite material described above, and to its use. The composite material is suitable, for example, for separation of substances, for example by filtration or adsorption, including chromatography, for use as a support in synthesis or for use as a support for cell growth.
    Type: Grant
    Filed: October 14, 2008
    Date of Patent: February 18, 2014
    Assignee: Natrix Separations Inc.
    Inventors: Ronald F. Childs, Carlos Filipe, Raja Ghosh, Alicja M. Mika, Jinsheng Zhou, Elena N. Komkova, Marcus Y. Kim, Tapan K. Dey
  • Patent number: 8569073
    Abstract: A test element, for example in the form of an immunological test strip functioning according to the sandwich principle, for the fluorophoric detection of one or more analytes in a sample comprising an analyte detection zone and a combined control and calibration zone. The combined control and calibration zone include a fluorophore and binding partners for the specific binding of reagents labelled with a fluorophore. Furthermore, the invention concerns a method for calibrating an analyte-specific measurement signal, a method for determining the concentration of an analyte in a sample and the use of the test element for calibrating a signal generated in the analyte detection zone of a test element.
    Type: Grant
    Filed: December 2, 2009
    Date of Patent: October 29, 2013
    Assignee: Roche Diagnostics Operations Inc.
    Inventor: Thomas Fischer
  • Patent number: 8563329
    Abstract: Modified branched polymers are combined with bioactive agents which are one member of a binding pair for use in an assay.
    Type: Grant
    Filed: May 2, 2006
    Date of Patent: October 22, 2013
    Assignee: ANP Technologies, Inc.
    Inventors: Ray Yin, Dujie Qin, Jing Pan
  • Patent number: 8563328
    Abstract: A method for biosensing that includes passing, via convective flow, a sample believed to contain one or more target biomarkers through a microfluidic channel and over the surface of an optical waveguide that has been prepared to bind the one or more target biomarkers, and sensing for an emission output from the optical waveguide at a wavelength that is characteristic of the binding of the target biomarker. A biosensor device that includes a module defining at least one microfluidic channel, an optical waveguide exposed along at least a portion of its length to fluid flow within the microfluidic channel, where a surface of the optical waveguide being prepared to bind a target biomarker, and an excitation source to couple an excitation wavelength of light into the optical waveguide. The device also includes a sensor for detecting emission light from the optical waveguide at an emission wavelength characteristic of binding of the target biomarker.
    Type: Grant
    Filed: February 16, 2006
    Date of Patent: October 22, 2013
    Assignee: University of Louisville Research Foundation, Inc.
    Inventors: Kyung Aih Kang, Chong H. Ahn
  • Patent number: 8557604
    Abstract: A lateral flow, membrane-based assay device for detecting the presence or quantity of an analyte residing in a test sample is provided. The device utilizes phosphorescence to detect the signals generated by excited phosphorescent labels. The labels may have a long emission lifetime so that background interference from many sources, such as scattered light and autofluorescence, is practically eliminated during detection. In addition, the phosphorescent labels may be encapsulated within particles to shield the labels from quenchers, such as oxygen or water, which might disrupt the phosphorescent signal.
    Type: Grant
    Filed: December 14, 2007
    Date of Patent: October 15, 2013
    Assignee: Kimberly-Clark Worldwide, Inc.
    Inventor: Xuedong Song
  • Patent number: 8557182
    Abstract: This invention provides molecularly imprinted polymers (MIPs) for the detection of analytes, methods for forming the MIPs and detecting the analyte using the MIPs. The MIP comprises templated sites which are formed using a mimic of the analyte such that a reporter compound can be selectively attached at the templated sites, thus providing a site selectively tagged and templated MIP.
    Type: Grant
    Filed: December 8, 2004
    Date of Patent: October 15, 2013
    Assignee: The Research Foundation of State University of New York
    Inventor: Frank V. Bright
  • Patent number: 8551759
    Abstract: An oligomer probe array having improved reaction yield is provided. The oligomer probe array includes a substrate, an immobilization layer on the substrate, a plurality of nano particles coupled with a surface of the immobilization layer, and a plurality of oligomer probes coupled with surfaces of the nano particles.
    Type: Grant
    Filed: March 15, 2007
    Date of Patent: October 8, 2013
    Assignee: Samsung Electronics Co., Ltd.
    Inventors: Jung-hwan Hah, Sung-min Chi, Kyoung-seon Kim, Won-sun Kim, Han-ku Cho, Sang-jun Choi, Man-hyoung Ryoo
  • Patent number: 8551786
    Abstract: Systems and methods for enhancing fluorescent detection of target molecules in a test sample are for use with an irradiating device. First fluorophores are provided for absorption of EMF radiation, and emission of a first signal. Second fluorophores are provided for partial absorption of the first signal, and emission of a second signal distinguishable from the first signal. The fluorophores are combined with the test sample, and secured to the target molecules and relative to one another. After the first fluorophores receive the EMF radiation from the irradiating device, the first signal is detected, together with the second spectral signal if the target molecules are present in the test sample.
    Type: Grant
    Filed: July 9, 2008
    Date of Patent: October 8, 2013
    Assignee: FIO Corporation
    Inventors: Warren Che Wor Chan, Travis Leon Jennings, Jesse M. Klostranec
  • Patent number: 8546130
    Abstract: An oligomer probe array with improved signal-to-noise ratio includes a substrate, a plurality of probe cell active regions formed on or in the substrate, with each of the plurality of probe cell active regions having a substantially planar surface and being coupled with at least one oligomer probe with own sequence, and a probe cell isolation region defining the probe cell active regions and having no functional groups for coupling with the oligomer probes on a surface.
    Type: Grant
    Filed: March 15, 2007
    Date of Patent: October 1, 2013
    Assignee: Samsung Electronics Co., Ltd.
    Inventors: Jung-hwan Hah, Sung-min Chi, Kyoung-seon Kim, Won-sun Kim
  • Patent number: 8524507
    Abstract: A method is provided for detecting a target molecule in a biological sample. One step of the method includes immobilizing the biological sample on a membrane. Next, the membrane-bound biological sample is contacted with at least one detection moiety. The membrane-bound biological sample is then separately mated with a substrate and the target molecule detected. At least one step of the method is performed under positive pressure or a vacuum.
    Type: Grant
    Filed: August 19, 2010
    Date of Patent: September 3, 2013
    Assignee: The Cleveland Clinic Foundation
    Inventor: David J. Kennedy
  • Patent number: 8513024
    Abstract: Provided is a detection method for a target substance capable of enhancing detection sensitivity and quantitative property of a magnetic biosensor, while keeping monodispersity and dispersion stability of magnetic markers, including the steps of: reacting the target substance in a sample solution with a first target substance trapping member immobilized on a sensing element and with a second target substance trapping member immobilized on a gel particle to hold the gel particle on the sensing element; adjusting a magnetic marker precursor including the gel particle and a magnetic material precursor existing in the gel particle by bringing the magnetic material precursor into contact with the gel particle; synthesizing a magnetic material from the magnetic material precursor held on the gel particle, thereby adjusting the magnetic markers; and detecting the magnetic markers with the sensing element.
    Type: Grant
    Filed: February 4, 2009
    Date of Patent: August 20, 2013
    Assignee: Canon Kabushiki Kaisha
    Inventor: Kazumichi Nakahama
  • Patent number: 8507212
    Abstract: A process for treating biological targets in a fluid of a biological organism, including introducing a fluid comprising a biological target to an assembly comprising an inlet connected to receive the fluid and an outlet connected to pass the fluid from the assembly, wherein the assembly comprises a flow chamber for conveying a flow of the fluid, and a capture zone comprising a target-specific binding agent, wherein during flow of the fluid through the flow chamber, the biological target undergoes flux rolling along the target-specific binding agent.
    Type: Grant
    Filed: January 10, 2012
    Date of Patent: August 13, 2013
    Assignee: Biomed Solutions LLC
    Inventors: Patrick R. Connelly, Jeffrey L. Helfer, Andrew W. Custer, Michael B. Kim
  • Patent number: 8501486
    Abstract: Protein phosphorylation is a major post-translational modification and it plays a pivotal role in numerous cellular functions. We present a composition that includes a soluble nanopolymer core functionalized with groups having an affinity for either metal ion or metal oxides which can be used for phosphopeptide enrichment. Exemplary compounds including PolyMAC-Zr, PolyMAC-Fe and PolyMAC-Ti demonstrate outstanding reproducibility, exceptional sensitivity, fast chelation time, and high phosphopeptide recovery from standard mixtures that include phosphorylated peptides. The composition can be used for phosphoproteome isolation from samples of medicinal, diagnostic or biological interest such as malignant breast cancer cells. Such compositions were used for the quantitative analysis of the changes in the tyrosine phosphoproteome in highly invasive breast cancer cells after induction of Syk kinase, a potent suppressor of tumor growth and metastasis.
    Type: Grant
    Filed: October 7, 2009
    Date of Patent: August 6, 2013
    Assignee: Purdue Research Foundation
    Inventor: Weiguo Andy Tao
  • Patent number: 8497137
    Abstract: Capture particles for harvesting analytes from solution and methods for using them are described. The capture particles are made up of a polymeric matrix having pore size that allows for the analytes to enter the capture particles. The pore size of the capture particles are changeable upon application of a stimulus to the particles, allowing the pore size of the particles to be changed so that analytes of interest remain sequestered inside the particles. The polymeric matrix of the capture particles are made of co-polymeric materials having a structural monomer and an affinity monomer, the affinity monomer having properties that attract the analyte to the capture particle. The capture particles may be used to isolate and identify analytes present in a mixture. They may also be used to protect analytes which are typically subject to degradation upon harvesting and to concentrate low an analyte in low abundance in a fluid.
    Type: Grant
    Filed: August 19, 2008
    Date of Patent: July 30, 2013
    Inventors: Alessandra Luchini, Lance Liotta, Emanuel Petricoin, Barney Bishop, Caterina Longo, Virginia Espina, Alexis Patanarut
  • Patent number: 8486717
    Abstract: The present invention relates to novel lateral flow devices using two dimensional features, preferably, uniform two dimensional test and control features, and the methods for detecting an analyte using the lateral flow devices, and processes for making the lateral flow devices.
    Type: Grant
    Filed: January 4, 2012
    Date of Patent: July 16, 2013
    Assignee: Symbolics, LLC
    Inventors: Brendan O'Farrell, Thomas C. Tisone
  • Patent number: 8481332
    Abstract: A liposomal composition, preferably a vaccine, comprising liposomes formed of liposome forming compounds, containing coentrapped polysaccharide antigen and T-cell dependent protein carrier, such as tetanus toxoid or diphtheria toxin modified to render it non-toxic. The invention is of use in the production of vaccines against Haemophilus influenzae, Streptococcus pneumoniae or Neisseria meningitidis.
    Type: Grant
    Filed: January 24, 2011
    Date of Patent: July 9, 2013
    Assignee: Stichting Voor de Technische Wetenshappen
    Inventors: Waltherus Jacobus Wilhelmus Van Venrooij, Jan Wouter Drijfhout, Martinus Adrianus Maria Van Boekel, Gerardus Jozef Maria Pruijn
  • Patent number: 8481326
    Abstract: A microfluidic device and method for measuring a level of cholesterol therewith are provided. The cholesterol measurement apparatus includes a microfluidic device including a plurality of chambers and at least one channel through which the plurality of chambers are interconnected. The plurality of chambers include a reaction chamber which contains a capture binder, a buffer chamber which contains an elution buffer and is connected to the reaction chamber, and at least one detection chamber which contains a cholesterol measurement reagent and is connected to the reaction chamber.
    Type: Grant
    Filed: July 8, 2011
    Date of Patent: July 9, 2013
    Assignee: Samsung Electronics Co., Ltd.
    Inventor: In Wook Kim
  • Patent number: 8481333
    Abstract: A method for determining the amount of NT-proBNP in blood samples from felines. The method includes detecting degradation products of feline NT-proBNP by various methods, including using antibodies, kits and devices.
    Type: Grant
    Filed: May 31, 2012
    Date of Patent: July 9, 2013
    Assignee: IDEXX Laboratories, Inc.
    Inventors: Mahalakshmi Yerramilli, Michael Atkinson, Murthy V. S. N. Yerramilli
  • Patent number: 8465968
    Abstract: The present invention relates to a label-free biosensor system, a method for manufacturing said label-free biosensor system, its use for detecting biochemical reactions and/or bindings, enzymatic reactions, nucleic acid hybridizations, protein-protein interactions and protein-ligand interactions, as well as an assay method for detecting and/or quantifying an analyte of interest in a biological sample which comprises detecting the Recognition Induced Birefringence (RIB) generated in the presence as opposed to the absence of said analyte by bringing said sample into contact with said label-free biosensor system.
    Type: Grant
    Filed: December 22, 2009
    Date of Patent: June 18, 2013
    Assignee: DWI an der RWTH Aachen e.V.
    Inventors: Jürgen Groll, Martin Möller, Matthias Eberhardt
  • Patent number: 8455265
    Abstract: A surface grafted conjugated polyelectrolyte (CPE) is formed by coupling a CPE by a coupling moiety to the surface of a substrate. The substrate can be of any shape and size, and for many uses of the surface grafted CPE, it is advantageous that the substrate is a nanoparticle or microparticle. Surface grafted CPEs are presented that use silica particles as the substrate, where a modified silane coupling agent connects the surface to the CPE by a series of covalent bonds. Two methods of preparing the surface grafted CPEs are presented. One method involves the inclusion of the surface being modified by the coupling agent and condensed with monomers that form the CPE in a grafted state to the substrate. A second method involves the formation of a CPE with terminal groups that are complimentary to functionality that has been placed on the surface of the substrate by reaction with a coupling agent. The surface grafted CPEs are also described for use as biosensors and biocides.
    Type: Grant
    Filed: February 29, 2008
    Date of Patent: June 4, 2013
    Assignee: STC.UNM
    Inventors: David G. Whitten, Sireesha Chemburu, Thomas Corbitt, Linnea Ista, Gabriel Lopez, Kirk S. Schanze, Motokatsu Ogawa, Eunkyung Ji
  • Patent number: 8454895
    Abstract: This invention refers to continuous flow devices for detecting and/or removal of contaminants from a liquid stream. The device comprises a cartridge with an inlet and outlet for the liquid stream, a radiation incident and emerging wall portion and optically transparent support material packed in the cartridge such that a liquid stream can pass between voids or spaces formed within the optically transparent material, as well as a radiation source and detector. The support material comprises molecules for capturing at least one contaminant on the surface of the support material and each of the capture molecules comprises at least one reporter group which emits a signal upon binding of the contaminant. Another form of the invention refers to a rotatable support within the liquid stream.
    Type: Grant
    Filed: May 2, 2008
    Date of Patent: June 4, 2013
    Assignee: Nanyang Technological University
    Inventors: Karina Yew-Hoong Gin, Trevor R. Garrett
  • Patent number: 8455263
    Abstract: The present invention relates to an assay device and a method for using such for the quantitative determination of an analyte, based on a test strip, which contains a porous test membrane allowing for capillary flow of the analyte and complexes of the analyte, a porous upstream membrane in fluid connection with the test membrane and a porous downstream membrane in fluid connection with the test membrane, wherein the test membrane contains a test site having immobilized thereon a ligand capable of reacting with the analyte and binding such to the test site, and two standard band sites having immobilized thereon known high and low concentrations of a calibrator agent capable of reacting with a label conjugate and binding such to the standard sites, wherein the upstream membrane has a site for the application of a sample to be analyzed, and has a site downstream from the sample application site for depositing label conjugates capable of reacting with the analyte and label conjugates capable of reacting with the
    Type: Grant
    Filed: December 30, 2008
    Date of Patent: June 4, 2013
    Inventor: Jin Po Lee
  • Patent number: 8426152
    Abstract: The present invention provides a method of detecting changes in the refractive index at the surface of a localized surface plasmon resonance (LSPR) detection system. The method includes generating an insoluble product from an enzymatic substrate using an immobilized enzyme, wherein the insoluble product accumulates at a LSPR supporting surface. The method also includes detecting changes in the reflected or transmitted light of the surface arising from the presence of the insoluble product using LSPR.
    Type: Grant
    Filed: December 28, 2007
    Date of Patent: April 23, 2013
    Assignee: LamdaGen Corporation
    Inventors: Daniele Gerion, Randy Storer, Hiroyuki Takei
  • Patent number: 8415113
    Abstract: A procedure for the identification of a functional disorder of the pancreas by the use of parts of all iso-enzymes of the pancreas elastase and of synthetic amino-acid sequences as antigens for obtaining specific antibodies, as well as their use in immuno-chemical test procedures.
    Type: Grant
    Filed: September 3, 1999
    Date of Patent: April 9, 2013
    Assignee: R-Biopharm AG
    Inventors: Hans-Werner Heinrich, Rainer Kleinert, Udo Meyer, Heinz-Jürgen Wagner
  • Patent number: 8415163
    Abstract: Disclosed is a method of diagnosing renal dysfunction in a cat by removing cauxin from cat urine and detecting a protein in the cat urine sample from which cauxin has been removed. Cauxin is removed from cat urine by bringing the cat urine into contact with a lectin or with an anti-cauxin antibody that specifically binds to cauxin. Cauxin may be removed using a column filled with a carrier that is bound to the lectin or to the anti-cauxin antibody. The lectin may be Lens culinaris lectin. Detection of the cat urinary protein from which cauxin has been removed is performed with a urine test strip.
    Type: Grant
    Filed: August 16, 2012
    Date of Patent: April 9, 2013
    Assignees: Incorporated National University Iwate University, Nippon Zenyaku Kogyo Co., Ltd.
    Inventors: Tetsuro Yamashita, Yasuyuki Suzuta
  • Patent number: 8394626
    Abstract: The present invention includes but is not limited to a specimen collection device that includes a chamber capable of collecting a specimen, a specimen passage slot, a reservoir, a reservoir seal, and a test device. A sample or specimen added to the chamber flows through the specimen passage slot into the reservoir. Flow into the reservoir may be limited by the reservoir seal. The test device positioned within the reservoir detects the presence or concentration of an analyte within the sample or specimen.
    Type: Grant
    Filed: June 2, 2009
    Date of Patent: March 12, 2013
    Assignee: Alere Switzerland GmbH
    Inventors: James T. Ramsey, Larry Hartselle