Tracers Or Tags Patents (Class 436/56)
-
Patent number: 7241381Abstract: The method for screening binding between a target binder and potential pharmaceutical chemicals involves sending a solution (preferably an aqueous solution) of the target binder through a conduit to a size exclusion filter, the target binder being too large to pass through the size exclusion filter, and then sending a solution of one or more potential pharmaceutical chemicals (preferably an aqueous solution) through the same conduit to the size exclusion filter after target binder has collected on the filter. The potential pharmaceutical chemicals are small enough to pass through the filter. Afterwards, x-rays are sent from an x-ray source to the size exclusion filter, and if the potential pharmaceutical chemicals form a complex with the target binder, the complex produces an x-ray fluorescence signal having an intensity that indicates that a complex has formed.Type: GrantFiled: November 10, 2004Date of Patent: July 10, 2007Assignee: Los Alamos National Security, LLCInventors: Benjamin P. Warner, George J. Havrilla, Thomasin C. Miller, Cyndi A. Wells
-
Patent number: 7235370Abstract: A method of making a derivatized aminoglycoside includes reacting an aminoglycoside with at least 2 equivalents of a divalent metal ion in an aprotic solvent to complex two neighboring amino group and hydroxyl group pairs; reacting the non-complexed amino groups with a protecting reagent to provide protecting groups; removing the divalent metal ion to provide two unprotected amino groups; reacting one of the unprotected amino groups with a reactive substance containing an linker, a carrier, or a label; and removing the protecting groups. This method can be used to produce novel compounds and reagents.Type: GrantFiled: July 22, 2003Date of Patent: June 26, 2007Assignee: Roche Diagnostics Operations, Inc.Inventors: Mitali Ghoshal, Salvatore J. Salamone
-
Patent number: 7232659Abstract: The present invention relates generally to molecular assemblies, more particularly to an assay for the detection of G-protein coupled receptor (GPCR) molecular assemblies and bead-based detection of ligand-GPCR complexes and to methods for identifying compounds as agonists, partial agonists or antagonists of the binding of G-protein coupled receptors to G-protein and for use of these compounds in the treatment of conditions or disease states in a mammalian subject or patient, including humans, where G-protein coupled receptor binding to G-protein is implicated.Type: GrantFiled: February 4, 2004Date of Patent: June 19, 2007Assignee: STC.UNMInventors: Larry A. Sklar, Eric R. Prossnitz, Peter Simons, Anna Waller, Daniel Cimino, Sean Biggs
-
Patent number: 7229842Abstract: Compositions are disclosed comprising (a) a metal chelate wherein the metal is selected from the group consisting of europium, terbium, dysprosium, samarium osmium and ruthenium in at least a hexacoordinated state and (b) a compound having a double bond substituted with two aryl groups, an oxygen atom and an atom selected from the group consisting of oxygen, sulfur and nitrogen wherein one of the aryl groups is electron donating with respect to the other. Such composition is preferably incorporated in a latex particulate material. Methods and kits are also disclosed for determining an analyte in a medium suspected of containing the analyte. The methods and kits employ as one component a composition as described above.Type: GrantFiled: May 19, 2005Date of Patent: June 12, 2007Assignee: Dade Behring Marburg GmbHInventors: Sharat Singh, Edwin F. Ullman
-
Patent number: 7230088Abstract: The invention discloses compounds and compositions for dual phototherapy and combined therapy and diagnosis of tumors and other lesions. The compounds have a Dye that, when photoactivated, operates via Type I and/or Type II mechanisms. Other Dye or azide components may operate by the same or different mechanisms. Selection of particular components in a compound, and formulation of the compound(s) in a composition permit different activation wavelengths to be used for different therapies. A targeting moiety may be added to the compound or composition so that the Dye locates at a particular site, such as a hormone-sensitive tumor, for diagnosis and/or treatment. The compounds and compositions may be incorporated within liposomes.Type: GrantFiled: October 14, 2003Date of Patent: June 12, 2007Assignee: Mallinckrodt, Inc.Inventors: Raghavan Rajagopalan, Samuel I. Achilefu, Joseph E. Bugaj, Richard B. Dorshow, Muthunadar P. Periasamy
-
Patent number: 7223553Abstract: Immunoassays for the detection of everolimus are provided. Compounds for producing antibodies for everolimus, as well as antibodies produced therefrom, are also provided.Type: GrantFiled: February 13, 2006Date of Patent: May 29, 2007Inventors: Mark Roberts, Lili Arabshahi, Jared Boyd, Christopher T. Dennis, Peter Marbach, George Aaron, Deng Hwang, Alexei Boris Shvets
-
Patent number: 7220383Abstract: An analytical apparatus to monitor fluid systems has at least one extraction module having a raw-sample reservoir connected by input fluid conduit to individual ones of said fluid systems, to extract raw samples for analysis from said fluid systems, one or more modification modules comprising additive materials to modify the extracted raw samples prior to analysis, an analytical device to receive at least a portion of said raw samples in an ordered sequence, and to determine concentration of at least one constituent of said sample portion, fluid-handling apparatus for transferring fluid through the analytical apparatus, and a computerized control and management system to manage operations of component modules and devices, and to report analytical results.Type: GrantFiled: February 28, 2002Date of Patent: May 22, 2007Assignee: Metara, Inc.Inventors: Marc R. Anderson, Larry N. Stewart, Howard M. Kingston
-
Patent number: 7217802Abstract: The invention provides a hapten, an immunogen comprising the hapten coupled to an antigenicity-conferring carrier material, a conjugate comprising the hapten coupled to a labelling agent, as well as, antibodies raised against the aforementioned immunogen and capable of binding with MDMA.Type: GrantFiled: December 20, 2002Date of Patent: May 15, 2007Assignee: Randox Laboratories LimitedInventors: Robert Ivan McConnell, Elouard Benchikh, Stephen Peter Fitzgerald, John Victor Lamont
-
Patent number: 7208451Abstract: A method and composition for identifying chemically tagged petroleum products can be achieved by adding one or more chemicals to a selected petroleum product wherein the chemical is immune to extraction from the petroleum product by conventional inexpensive absorbents, cannot be removed by extraction with acids, bases, or immiscible solvents, cannot be easily oxidized, reduced or reacted with common agents, is difficult to disguise by masking with other agents, has a low polarity, and has a boiling point in the range of the petroleum product the chemical is being added to. The presence of the chemical is determined by using ion mobility spectroscopy.Type: GrantFiled: January 29, 2003Date of Patent: April 24, 2007Assignee: Authentic Inc.Inventors: W. Dale Spall, Karen Mehlin
-
Patent number: 7205116Abstract: Novel conjugates of 5-fluoro-uracil and novel 5-fluoro-uracil immunogens and monoclonal antibodies generated by these immunogens which are useful in immunoassays for the quantification and monitoring of 5-fluoro-uracil in biological fluids.Type: GrantFiled: February 2, 2006Date of Patent: April 17, 2007Inventors: Salvatore J. Salamone, Jodi Blake Courtney, Dennis Stocker
-
Patent number: 7192722Abstract: The present invention relates to a method for preparing haptens of formulae I and II that are useful in the preparation of immunogens, antibodies and conjugates, for use in competitive immunoassays for the detection of ractopamine, isoxsuprine and ritodrine. The haptens are prepared by reacting a phenylethanolamine derivative of formula D with a phenylalkylcarbonyl derivative of formula E: in which, in formula I, Z1 is a crosslinker and Z2 is H and, in formula II, Z1 is H and Z2 is a crosslinker.Type: GrantFiled: November 10, 2005Date of Patent: March 20, 2007Assignee: Randox LaboratoriesInventors: Robert Ivan McConnell, Stephen Peter Fitzgerald, El Ouard Benchikh, Andrew Philip Lowry
-
Patent number: 7192778Abstract: Metal nanoparticles associated with a spectroscopy-active (e.g., Raman-active) analyte and surrounded by an encapsulant are useful as sensitive optical tags detectable by surface-enhanced spectroscopy (e.g., surface-enhanced Raman spectroscopy).Type: GrantFiled: January 16, 2003Date of Patent: March 20, 2007Inventor: Michael J. Natan
-
Patent number: 7192721Abstract: A universal test apparatus for detecting tuberculosis (TB) in any of many different species of non-primate mammals is provided. The universal test apparatus includes a test site having a mycobacterial antigen cocktail comprising ESAT-6/CFP10 and 16 kDa/MPB83 polyfusion antigens, and a tracer having the ESAT-6/CFP10 polyfusion antigen and MPB83 antigen conjugated to latex or colloidal gold. The universal test apparatus is effective across genuses, families and orders of non-primate mammals.Type: GrantFiled: September 8, 2005Date of Patent: March 20, 2007Assignee: Chembio Diagnostic Systems, Inc.Inventors: Javanbakhsh Esfandiari, Konstantin Lyashchenko
-
Patent number: 7193705Abstract: A method for determining atomic isotope masses in mass spectrometry, atomic isotope ratios being determined from molecular isotope ratios measured by means of an isotope mass spectrometer—ion correction, the determination of the atomic ratios being carried out by setting up and solving a system of equations which describes relationships between the atomic and the molecular ratios, and the system of equations having to have at least as many independent equations as there are atomic ratios. The entire system of equations is linearized by means of suitable numerical methods in a first step, in particular by means of a Taylor expansion or similar method, and in which the linearized system of equations is subsequently solved as a whole without transforming the individual equations.Type: GrantFiled: September 8, 2004Date of Patent: March 20, 2007Assignee: Thermo Electron (Bremen) GmbHInventor: Hans-Jürgen Schlüter
-
Patent number: 7189582Abstract: Compounds are disclosed comprising a moiety, such as a poly(amino acid), a non-poly(amino acid) label moiety, or a non-poly(amino acid) immunogenic carrier, linked to a sirolimus compound at position 26 or at position 32. Such sirolimus compounds comprising an immunogenic carrier can be employed to raise both polyclonal and monoclonal antibodies to the sirolimus compound. Polyclonal antibodies are also disclosed, which are raised against a compound wherein a moiety, such as a poly(amino acid), a non-poly(amino acid) label moiety, or a non-poly(amino acid) immunogenic carrier, is linked to a sirolimus compound at position 32. The above antibodies and sirolimus compounds comprising a label can be used in assays for the detection of sirolimus compounds.Type: GrantFiled: April 27, 2005Date of Patent: March 13, 2007Assignee: Dade Behring Inc.Inventors: Jian Chen, Holger Keim, Hshiou-ting Liu, Yi Feng Zheng, Yali Yang, Cathy K. Worley
-
Patent number: 7189519Abstract: The present invention provides a general approach for G protein coupled receptors that may be used to define agonists and antagonists, and the specificity of receptor coupling to G protein subunits. Methods of the present invention use small volumes (microliters) and are compatible with high throughput flow cytometry. When assays of the present invention are multiplexed, the specificity of the interactions of a receptor with many G proteins may be determined simultaneously.Type: GrantFiled: May 5, 2003Date of Patent: March 13, 2007Assignees: STC.UNM, Univeristy of MichiganInventors: Larry A. Sklar, Tione Buranda, Daniel Cimino, Alex T. Key, Richard Neubig, Peter C. Simons, Eric R. Prossnitz, Mei Shi
-
Patent number: 7180056Abstract: In a method of measuring by a tandem mass spectrometer a sample labeled with an isotope, measuring throughput is improved. In a technique in which tandem mass spectrometer is used to analyze a sample labeled with an isotope, spectra obtained by a first-stage measurement (MS1) are analyzed during a measuring session in a realtime fashion to determine ions to be used in second-stage and subsequent dissociation• spectral measurement (MS2).Type: GrantFiled: June 3, 2005Date of Patent: February 20, 2007Assignee: Hitachi, Ltd.Inventors: Atsushi Ohtake, Kinya Kobayashi, Kiyomi Yoshinari, Toshiyuki Yokosuka, Atsumu Hirabayashi
-
Patent number: 7163827Abstract: A compound represented by formula I is used as a marker for organic products. wherein Ar1 and Ar2 each independently represent a substituted or unsubstituted phenylene group or a substituted or unsubstituted naphthylene group; R1 represents a straight or branched chain alkyl group having 1 to 22 carbon atoms; R2 represents a hydrogen atom or a group of the formula C(O)R4 where R4 is a hydrogen atom or a straight or branched chain alkyl group having 1 to 22 carbon atoms; and R3 represents a hydrogen atom, a straight or branched chain alkyl group having 1 to 12 carbon atoms, a straight or branched chain alkoxy group having 1 to 12 carbon atoms, a hydroxyl group, or a substituted or unsubstituted phenyl group or a substituted or unsubstituted naphthyl group; and Z represents a hydrogen atom or a group of atoms that combine with Ar2 or R3 to form a lactone ring.Type: GrantFiled: November 6, 2002Date of Patent: January 16, 2007Assignee: United Color Manufacturing, Inc.Inventors: Michael J. Smith, Bharat Desai
-
Patent number: 7160437Abstract: The present invention relates to a method for determining the source of fouling in petroleum thermal conversion process units. More particularly, the invention distinguishes whether fouling occurs due to feed entrainment of small feed droplets or vapor phase condensation.Type: GrantFiled: October 8, 2003Date of Patent: January 9, 2007Assignee: ExxonMobil Research and Engineering CompanyInventors: Leo D. Brown, Peter S. Maa, William N. Olmstead, Michael Siskin
-
Patent number: 7161023Abstract: A system for recovering a diacetylenic monomer shelf life monitoring compound of reduced particle size and narrow size distribution range which comprises quenching a solution of the monomer by mixing the solution in a lower temperature quenching fluid having a base temperature which is at least about 30° C. below the nominal temperature at which the monomer compound recrystallizes from solution, the mixing being effected in such a manner as to limit the resulting quenching mixture temperature to one which is not higher than a maximum of about 15° C. above the quenching fluid base temperature.Type: GrantFiled: July 28, 2004Date of Patent: January 9, 2007Assignee: Temptime CorporationInventors: Thaddeus Prusik, Dawn E. Smith, Ingo H. Leubner, Wesley A. Olmsted
-
Patent number: 7157611Abstract: A method for marking a liquid petroleum hydrocarbon. The method comprises adding to the liquid petroleum hydrocarbon a pyrazinoporphyrazine dye having an absorption maximum from 700 nm to 900 nm.Type: GrantFiled: June 19, 2003Date of Patent: January 2, 2007Assignee: Rohm and Haas CompanyInventors: Rajiv Manohar Banavali, Kim Sang Ho
-
Patent number: 7148069Abstract: The present invention provides a method for the analysis of a compound with amino group (e.g., an amino acid or peptide) contained in a sample and convenient manner with a high sensitivity. The compound with amino group in a sample containing the compound with amino group is labeled with a specific carbamate compound such as p-trimethylammonium anilyl-N-hydroxysuccinimidyl carbamate iodide to enhance the selectivity and sensitivity. The present invention is preferably used in conjunction with mass spectrometry such as MS/MS method to facilitate quantitative analysis. The present invention further provides labeling reagents for mass spectrometry.Type: GrantFiled: August 16, 2004Date of Patent: December 12, 2006Assignee: Ajinomoto Co., Inc.Inventors: Hiroshi Miyano, Akihisa Yahashi, Kazutaka Shimbo, Masakazu Nakazawa, Kazuo Hirayama
-
Patent number: 7148066Abstract: Systems and methods providing for the introduction of a dye, particularly a xanthene dye, and more particularly a rhodamine dye, to liquid anhydrous ammonia to discourage theft of the anhydrous ammonia and provide for leak detection in storage vessels. The dye will stain objects which come into contact with the liquid anhydrous ammonia allowing for the detection of such contact. Generally, the staining will be visible to the naked eye, but may also fluoresce when exposed to a particular light source such as ultra violet (UV) light.Type: GrantFiled: August 2, 2004Date of Patent: December 12, 2006Assignee: GloTell Products, Inc.Inventors: Tim Bickett, Frank Eiter
-
Patent number: 7144971Abstract: A polycarbonate generated from the polymerization of a protected hydroxylated bisphenolic compound, a bisphenol, a monophenolic endcapping agent, a bisphenol haloformate, and a curing compound, and thereafter subjecting the obtained polymer to a reaction with an acidic compound. A hydroxylated charge transport compound may also be included in the polycarbonate.Type: GrantFiled: August 4, 2004Date of Patent: December 5, 2006Assignee: Xerox CorporationInventors: Timothy P. Bender, H Bruce Goodbrand
-
Masking of the background fluorescence and luminescence in the optical analysis of biomedical assays
Patent number: 7138280Abstract: In a process for the quantitative optical analysis of biological cells labelled with a fluorescent dye, the sensitivity of analytical detection can be considerably improved if a masking dye, which absorbs the excitation light for the fluorescent dye and/or its emission light is added to the solution surrounding the biological cells and/or if a separating layer permeable to the solution and absorbing and/or reflecting the excitation light or the emission light is applied to a layer of the biological cells at the bottom of a reaction vessel. This process can also be used for improving the sensitivity in the quantitative optical analysis of a luminescent biological cell layer. Analogously, these process principles can also be used in receptor studies for the masking of the interfering background radiation in the quantitative optical analysis of fluorescently or luminescently labelled reaction components.Type: GrantFiled: October 3, 2002Date of Patent: November 21, 2006Assignee: Bayer Healthcare AGInventors: Thomas Krahn, Wolfgang Paffhausen, Andreas Schade, Martin Bechem, Delf Schmidt -
Patent number: 7132079Abstract: This invention relates to methods for determining whether or not to discard a sample of oil based on the presence of polar compounds in the oil and devices to be used therewith. In a preferred embodiment a test strip comprising an adsorbent affixed to a backing is spotted with a polar indicator. A sample of oil is positioned on a heat conducting device supporting the test strip and the oil is allowed to migrate over the adsorbent material thereby displacing the polar indicator in an amount related to the amount of polar compounds in the oil. This displacement is correlated to the amount of polar compounds in the sample.Type: GrantFiled: June 24, 2002Date of Patent: November 7, 2006Assignee: 3M Innovative Properties CompanyInventors: Fidelis C. Onwumere, Robert A. Pranis, Jack G. Truong
-
Patent number: 7122377Abstract: The method of the invention provides novel compounds, termed acid-labile isotope-coded extractants (ALICE), for quantitative mass spectrometric analysis of protein mixtures. The compounds contain a thiol-reactive group that is used to capture cysteine-containing peptides from all peptide mixtures, an acid-labile linker, and a non-biological polymer. One of the two acid-labile linkers is isotopically labeled and therefore enables the direct quantitation of peptides/proteins through mass spectrometric analysis. Because no functional proteins are required to capture peptides, a higher percentage of organic solvent can be used to solubilize the peptides, particularly hydrophobic peptides, through the binding, washing and eluting steps, thus permitting much better recover of peptides. Moreover, since the peptides are covalently linked to the non-biological polymer (ALICE), more stringent washing is allowed in order to completely remove non-specifically bound species.Type: GrantFiled: August 17, 2004Date of Patent: October 17, 2006Assignee: Genetics Institute, LLCInventors: Yongchang Qiu, Jack H. Wang, Rodney M. Hewick
-
Patent number: 7118919Abstract: Use of 13C glucose in an analytical assay to monitor glucose metabolism by measurement of labeled exhaled CO2 is provided. A breath test and kit for performing the breath test are described for the diagnosis of diabetic indications and monitoring of glycemic control. The breath test utilizes the measurement of expired 13C-labeled CO2 following the ingestion of a 13C-enriched glucose source.Type: GrantFiled: February 28, 2005Date of Patent: October 10, 2006Assignee: Isotechnika Inc.Inventors: Randall W. Yatscoff, Robert T. Foster, Launa J. Aspeslet, Richard Lewanczuk
-
Patent number: 7112445Abstract: The present invention relates to identification tagging, and is specifically directed to identification tagging of ammunition. An isotopic taggant is deposited in a layer at the interface between the primer and the propellant so that, as the ammunition is fired, the taggant is dispersed throughout the propellant. The taggant is thus contained in the gunshot residue formed during the firing, and can be read by analysis of residue particles. Alternatively, the taggant may be deposited in a layer under the primer reactants, or in pellets which are easily destroyed by the chemical reactions involved in firing the ammunition, again dispersing the taggant throughout the propellant and the gunshot residue. Non-isotopic chemical taggants may also be employed if they are encoded so as to minimize the possibility of the information being destroyed or improperly read after the taggants are exposed to the chemical reactions in firing the ammunition.Type: GrantFiled: May 19, 2000Date of Patent: September 26, 2006Inventor: Richard P Welle
-
Patent number: 7101719Abstract: Disclosed is a method for the measurement of a cellular process, or for the measurement of the effect of a test compound on a cellular process, in one or more different populations of cells. The method comprises providing separate samples of one or more different populations of cells adhering to support particles, the support particles comprising a scintillant substance and being adapted for cell growth. In one embodiment, different samples of cells are introduced into separate reaction vessels in a fluid medium, together with a reagent labelled with a radioisotope, in the presence or the absence of the test compound, under conditions so as to cause a portion of said radiolabelled reagent to become associated with the cells. In another embodiment, multiparameter analysis may be performed to determine the effect of a test compound on a cellular process using two or more different cell populations present in the same well.Type: GrantFiled: November 5, 2001Date of Patent: September 5, 2006Assignee: GE Healthcare LimitedInventors: Gerard Bernard O'Beirne, Rahman Aziz Ismail, Nicholas Thomas
-
Patent number: 7094364Abstract: In one embodiment, a method for authenticating that a test polymer is an authenticatable polymer is disclosed wherein the authenticatable polymer comprises a substrate polymer and a thermochromic compound, the thermochromic compound having a first signal at a first temperature and at an authenticating wavelength, and a second signal at an authenticating temperature and the authenticating wavelength, the first and second signals being different, and the authenticating temperature being greater than the first temperature, the method comprising subjecting the test polymer to a stimulus sufficient to raise a portion of the test polymer to the authenticating temperature to create a heated portion, determining a test signal of the heated portion of the test polymer at the authenticating wavelength, and authenticating that the test polymer is an authenticatable polymer if the test signal is the same as an authenticating signal of the authenticatable polymer.Type: GrantFiled: November 26, 2003Date of Patent: August 22, 2006Assignee: General Electric CompanyInventors: Radislav Potyrailo, Philippe Schottland
-
Patent number: 7063952Abstract: In a process for the quantitative optical analysis of fluorescently labelled biological cells 5, a cell layer on a transparent support at the bottom 2 of a reaction vessel 1 is in contact with a solution 3 containing the fluorescent dye 4. The sensitivity of analytical detection can be considerably improved if to the fluorescent dye 4 already present in addition a masking dye 9, which absorbs the excitation light 6 for the fluorescent dye 4 and/or its emission light 7, is added to the solution 3 and/or if a separating layer 10 permeable to the solution and absorbing and/or reflecting the excitation light 6 or the emission light 7 is applied to the cell layer at the bottom 2. This process can also be used for improving the sensitivity in the quantitative optical analysis of a luminescent biological cell layer. The separating layer 10 must in this case be composed such that it has a high power of reflection for the luminescent light 11.Type: GrantFiled: September 28, 2001Date of Patent: June 20, 2006Assignee: Bayer Healthcare AGInventors: Thoams Krahn, Wolfgang Paffhausen, Andreas Schade, Martin Bechem, Delf Schmidt
-
Patent number: 7052916Abstract: Methods for incorporating a stable isotope into a protein or peptide fragment are disclosed. The methods include providing isolated isotope-labeled protein or peptide fragments and analyzing the isolated labeld fragments. In another aspect of the invention, methods for quantitatively comparing peptides in two protein or peptide fragment mixtures are provided. In these methods, protein levels are measured using stable-isotope coded protein or peptide fragments.Type: GrantFiled: May 24, 2002Date of Patent: May 30, 2006Assignee: Immunex CorporationInventor: Richard S. Johnson
-
Patent number: 7047830Abstract: A method and an apparatus for detecting, locating, and quantifying contamination in a fluid flow system like a pipe or duct. This characterization technique uses a conservative and one or more interactive tracers that are injected into the fluid flow system and then monitored at another location in the system. Detection, location, and quantification are accomplished by analysis of the characteristic features of measured curves of tracer concentration.Type: GrantFiled: July 10, 2003Date of Patent: May 23, 2006Assignee: Vista Engineering Technologies, Inc.Inventors: Wesley L. Bratton, Joseph W. Maresca, Jr.
-
Patent number: 7049149Abstract: The object of the invention is to propose a novel biomarker for stress states, which biomarker is highly meaningful and capable of enabling quantitative measurements to be made of stress levels. To do this, it has been found, surprisingly, that the air at the end of a person's breathing out, known as “alveolar” air, contains fluorine compounds when that person has been subjected to various stress states. More precisely, the present invention provides components based on fluorine compounds, in particular fluorinated chloroalkanes, used as biomarkers of stress states.Type: GrantFiled: October 18, 2002Date of Patent: May 23, 2006Assignee: AR2I SA - Analyses - Recherches et Innovation InstrumentaleInventors: Christian Sarbach, Pascal Delvordre, Eric Postaire
-
Patent number: 7049148Abstract: A photoactive moiety exhibiting an anisotropic transition dipole. The moiety exhibits emission of polarized light in response to energy absorption. In a preferred embodiment, the moiety comprises a particle from the group consisting of a crystalline arrangement of photoactive molecules and a photoactive nanocrystal. The moiety may include a matrix in which photoactive objects exhibiting an anisotropic emission dipole are embedded. The moiety may be photobleached to product the anisotropy and the photoactive objects may have a one dimensional transition dipole in their natural state.Type: GrantFiled: December 19, 2003Date of Patent: May 23, 2006Assignee: Massachusetts Institute of TechnologyInventors: Moungi Bawendi, Stephen Empedocles
-
Patent number: 7048179Abstract: An apparatus for electronically determining whether a tax or duty has been paid for the contents of that container. A radio frequency identification (RFID) tag is placed on the container which includes information indicative of whether the tax has been paid. The RFID tag includes a substrate, a radio frequency transceiver circuit mounted upon the substrate and connected to an antenna having a fixed length that is also mounted upon the substrate, and, preferably, a protrusion affixed to or a slot in the substrate. Preferably, the RFID tag is placed within the container's cap, such that, when mounted on the container, the cap mates with a ratchet wheel connected to the container that engages the protrusion or slot such that removing the cap from the container will cause the substrate to fracture and thereby cause the RFID tag to become permanently disabled.Type: GrantFiled: February 13, 2004Date of Patent: May 23, 2006Inventors: Francis M. Claessens, Timo W. Kipp, John P. Palmer
-
Patent number: 7026167Abstract: The present invention relates to a method of applying mass spectrometry to analyzing peptides or proteins, especially in the proteome setting. More particularly, the invention relates to a mass spectrometry-based method for detection of protein/peptide phosphorylation wherein the side chains of glutamic acid and/or aspartic acid residues of said peptides or proteins are chemically modified as to improve the selectivity/efficiency of identification of the phosphorylated protein/peptide.Type: GrantFiled: December 26, 2002Date of Patent: April 11, 2006Assignee: University of Virginia Patent FoundationInventors: Donald F. Hunt, Forest M. White, Scott Ficarro
-
Patent number: 7008794Abstract: A method and apparatus for assay of multiple analytes. The method uses a sensing element comprising a substrate upon which is arranged a multiplicity of recognition elements, such that each element is laid out in a predetermined pattern. Each pattern is unique in that it can give rise to a characteristic diffraction pattern in the assay. The patterns may or may not be interpenetrating on the substrate surface. The method of detecting multiple analytes includes contacting the medium of analytes with the patterned substrate, illuminating the substrate by a light source, and detecting any resultant diffraction image. The pattern of diffraction and the intensity of the diffracted signal provides information about the existence of specific analytes and their quantification.Type: GrantFiled: March 22, 2001Date of Patent: March 7, 2006Assignee: Axela Biosensors Inc.Inventors: M. Cynthia Goh, Jane B. Goh, Richard McAloney, Richard Loo
-
Patent number: 6977177Abstract: A method for marking a liquid petroleum hydrocarbon. The method comprises adding to the liquid petroleum hydrocarbon at least one substituted anthraquinone dye having formula (I) wherein R1 and R2 independently are hydrogen, hydroxy, OR11, amino or NR11R12; R3 and R5 independently are alkyl, aryl, aralkyl, heteroalkyl or heterocyclic; R4 and R6 independently are hydrogen or alkyl; R7, R8, R9 and R10 independently are cyano, nitro or hydrogen, provided that at least two of R7, R8, R9 and R10 are cyano or nitro; R11 is alkyl, aryl, aralkyl, heteroalkyl, heterocyclic or alkanoyl; R12 is hydrogen or alkyl; and wherein the substituted anthraquinone dye(s) has an absorption maximum in the range from 690 nm to 1000 nm.Type: GrantFiled: May 26, 2004Date of Patent: December 20, 2005Assignee: Rohm and Haas CompanyInventors: Kim Sang Ho, David Roderick Baxter
-
Patent number: 6972198Abstract: The invention relates to a method for determining the conformational state of a protein, comprising the steps of: a) providing a first binding partner which is capable of binding to the protein in a manner dependent on the conformational state of the protein and which generates a signal in a manner dependent on the binding of the first binding partner to the protein; and b) contacting the protein with the first binding partner and determining the conformational state of the protein by assessing the labelling of the protein by the binding of the first binding partner.Type: GrantFiled: February 24, 2000Date of Patent: December 6, 2005Assignee: Cyclacel, Ltd.Inventors: Roger K. Craig, John Colyer
-
Patent number: 6962789Abstract: The present invention provides a method for determining expression levels of one or a multiplicity of target proteins in a tissue or cell sample.Type: GrantFiled: January 12, 2001Date of Patent: November 8, 2005Assignee: Ventana Medical Systems, Inc.Inventor: Sarah S. Bacus
-
Patent number: 6933109Abstract: Methods and reagents for rapid purification and/or identification of particles in a liquid sample are described. The technique uses centrifugation to concentrate particles against a slanted surface having an agent specifically binding to the particles. This method is applicable for the rapid identification of viruses and other difficult or impossible to culture microorganisms without replication or amplification of the microorganism.Type: GrantFiled: December 22, 2000Date of Patent: August 23, 2005Assignee: Large Scale Proteomics CorporationInventor: Norman G. Anderson
-
Patent number: 6927065Abstract: A method for determining the length and velocity of single elongated macromolecules is disclosed. In particular, the present invention relates to methods and apparatus for determining the velocity of elongated polymeric molecules moving relative to one or more detection stations, as well as to methods and apparatus for determining the length of such molecules and the distance between landmarks that may be present on such molecules. The invention makes use of time correlated measurements of signal amplitude profiles that result from interactions between each detection station and portions of each macromolecule.Type: GrantFiled: June 6, 2001Date of Patent: August 9, 2005Assignee: U.S. Genomics, Inc.Inventors: Eugene Y. Chan, Lance C. Gleich, Parris S. Wellman
-
Patent number: 6911313Abstract: A method for discriminating and counting erythroblasts comprises the steps of: (i) staining leukocytes in a hematologic sample by adding a fluorescent labeled antibody capable of binding specifically with leukocytes to the hematologic sample; (ii) raising the permeability only of cell membranes of erythroblasts in the hematologic sample to a nucleotide fluorescent dye which does not permeate a cell membrane usually, the nucleotide fluorescent dye having a fluorescent spectrum capable of being distinguished from that of a fluorescent labeling compound of the fluorescent labeled antibody in step (i); (iii) staining nuclei of the erythroblasts in the hematologic sample with the nucleotide fluorescent dye; (iv) subjecting the hematologic sample to flowcytometry to detect at least two fluorescent signals from each cell; and (v) discriminating and counting the erythroblasts from difference in intensity between the at least two fluorescent signals.Type: GrantFiled: April 9, 1998Date of Patent: June 28, 2005Assignee: Sysmex CorporationInventors: Berend Houwen, Fu-sheng Wang, Tomohiro Tsuji, Takashi Sakata, Yukio Hamaguchi
-
Patent number: 6905879Abstract: Arginine-containing cysteine-modifying compounds useful for MALDI-MS analysis of proteins are provided. These compounds termed isotope-coded ionization enhancement reagents (ICIER) can provide ionization enhancement in MALDI-MS, relative quantitation, and additional database searching constraints at the same time without any extra sample manipulation. More specifically, ICIER increase the ionization efficiency of cysteine-containing peptides by attachment of a guanidino functional group. ICIER also increase the overall hydrophilicity of these peptides due to the hydrophilic nature of ICIER and thus increase the percentage of recovery of these peptides during sample handling and processing such as in-gel digestion or liquid chromatography.Type: GrantFiled: October 22, 2001Date of Patent: June 14, 2005Assignee: Genetics Institute, Inc.Inventors: Yongchang Qiu, Jack H. Wang, Rodney M. Hewick
-
Patent number: 6902936Abstract: The method of the invention provides novel compounds, termed acid-labile isotope-coded extractants (ALICE), for quantitative mass spectrometric analysis of protein mixtures. The compounds contain a thiol-reactive group that is used to capture cysteine-containing peptides from all peptide mixtures, an acid-labile linker, and a non-biological polymer. One of the two acid-labile linkers is isotopically labeled and therefore enables the direct quantitation of peptides/proteins through mass spectrometric analysis. Because no functional proteins are required to capture peptides, a higher percentage of organic solvent can be used to solubilize the peptides, particularly hydrophobic peptides, through the binding, washing and eluting steps, thus permitting much better recovery of peptides. Moreover, since the peptides are covalently linked to the non-biological polymer (ALICE), more stringent washing is allowed in order to completely remove non-specifically bound species.Type: GrantFiled: October 22, 2001Date of Patent: June 7, 2005Assignee: Genentics Institute, Inc.Inventors: Yongchang Qiu, Jack H. Wang, Rodney M. Hewick
-
Patent number: 6900023Abstract: A method for classifying and counting leukocytes comprises the steps of: (1) adding to a hematological sample the following fluorescence-labeled antibodies labeled with fluorescent dyes which emit fluorescences distinguishable from each other; (a) a first fluorescence-labeled antibody (1st antibody) which bonds specifically to leukocytes, (b) a second fluorescence-labeled antibody (2nd antibody) which bonds to at least one kind of neutrophilic cells, and (c) a third fluorescence-labeled antibody (3rd antibody) which bonds to at least one kind of immature granulocytic cells, in order to stain leukocytic cells in the sample, and removing erythrocytes from the sample; (2) analyzing the resulting sample using a flow cytometer to measure at least one scattered light signal and three separate fluorescence signals; (3) defining a group of granulocytic cells on the basis of intensity of the scattered light and intensity of fluorescence from the 1st antibody; (4) defining neutrophilic cells in the defined group of graType: GrantFiled: September 2, 1999Date of Patent: May 31, 2005Assignee: Sysmex CorporationInventors: Berend Houwen, Fu-sheng Wang, Hiroyuki Fujimoto, Takashi Sakata, Yukio Hamaguchi
-
Patent number: 6893875Abstract: A method for assay of an analyte by use of a material labeled with a chemiluminescent substance, which comprises adding a quencher and/or decreasing the specific activity of a chemiluminescent substance labeled probe, thereby decreasing the quantity of chemiluminescence.Type: GrantFiled: May 10, 1999Date of Patent: May 17, 2005Assignee: Chugai Seiyaku Kabushiki KaishaInventors: Yasuhiro Tsuji, Keiichi Kamisango, Mitsuo Otsuka
-
Patent number: 6893814Abstract: A sensor for the detection of tetrameric multivalent neuraminidase within a sample is disclosed, where a positive detection indicates the presence of a target virus within the sample. Also disclosed is a trifunctional composition of matter including a trifunctional linker moiety with groups bonded thereto including (a) an alkyl chain adapted for attachment to a substrate, (b) a fluorescent moiety capable of generating a fluorescent signal, and (c) a recognition moiety having a spacer group of a defined length thereon, the recognition moiety capable of binding with tetrameric multivalent neuraminidase.Type: GrantFiled: August 28, 2003Date of Patent: May 17, 2005Assignee: The Regents of the University of CaliforniaInventors: Basil I. Swanson, Xuedong Song, Clifford Unkefer, Louis A. Silks, III, Jurgen G. Schmidt