Abstract: Compositions are disclosed comprising (a) a metal chelate wherein the metal is selected from the group consisting of europium, terbium, dysprosium, samarium osmium and ruthenium in at least a hexacoordinated state and (b) a compound having a double bond substituted with two aryl groups, an oxygen atom and an atom selected from the group consisting of oxygen, sulfur and nitrogen wherein one of the aryl groups is electron donating with respect to the other. Such composition is preferably incorporated in a latex particulate material. Methods and kits are also disclosed for determining an analyte in a medium suspected of containing the analyte. The methods and kits employ as one component a composition as described above.

Abstract: Plasticizer-free ion-detecting sensors for detecting a target ion in a sample are provided. The sensor comprises a plasticizer-free copolymer comprised of polymerized units of methacrylate monomers and a polymerizable ion exchanger, wherein the methacrylated monomers have pendent alkyl groups of different length and wherein the functionalized ion-exchanger is grafted into the copolymer through covalent linkages. The ion exchanger comprises a C-derivative of a halogenated closo-dodecacarborane anion having a polymerizable moiety. Sensors of this invention include carrier-based ion-selective electrodes or optodes such as thin film ion-specific optodes, particle-based optodes, or bulk optodes.

Abstract: An analytical apparatus to monitor fluid systems has at least one extraction module having a raw-sample reservoir connected by input fluid conduit to individual ones of said fluid systems, to extract raw samples for analysis from said fluid systems, one or more modification modules comprising additive materials to modify the extracted raw samples prior to analysis, an analytical device to receive at least a portion of said raw samples in an ordered sequence, and to determine concentration of at least one constituent of said sample portion, fluid-handling apparatus for transferring fluid through the analytical apparatus, and a computerized control and management system to manage operations of component modules and devices, and to report analytical results.

Abstract: The invention concerns scintillation proximity assays performed in multiwell plates where a charge coupled device is used to image the wells. Conventional phosphors emit blue light (350–450 nm) which is absorbed by yellow or brown assay components. This problem is addressed by the use of phosphors that emit radiation of longer wavelength (480–900 nm).

Abstract: The present invention provides compositions and methods for detecting, analyzing, and identifying biomolecules. More particularly, the invention provides Element Coded Affinity Tags comprising a metal chelate and a metal ion and methods of using the tags to detect, analyze, and identify biomolecules including polypeptides, nucleic acids, lipids, and polysaccharides.

Abstract: A plasticizer-free ion detective sensor for detecting a target ion in a sample is provided. The sensor comprises a copolymer of methacrylate monomers with pendant alkyl groups of different length, and an ionophore for detecting the target ion. The copolymer matrix of the present invention may be in a form of membrane or particles. The sensors of the present invention may be Carrier-based ion-selective electrodes (ISEs) or optodes such as thin film ion-specific optodes or particle-based optodes. The ionophore may be a target ionophore selective for a target ion H+, Li+, Na+, K+, Ca2+, or Mg2+. The ion detective sensor of the present invention may further include an ion exchanger such as halogenated carboranes. Also provided is an ion detective sensor comprising halogenated carboranes as ion exchangers. Particularly, trimethylammonium-2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12 undecabromocarborane (TMAUBC) is used as ion exchangers.

Abstract: Ion-detecting sensors for detecting a target ion in a sample are provided. The sensor comprises a plasticizer-free copolymer comprised of polymerized units of methacrylate monomers having pendent alkyl groups of different length and a functionalized ionophore of said ion, wherein at least a portion of the functionalized ionophore is grafted into the copolymer through covalent linkages. Sensors may comprise ionophores such as hydrophilic crown ethers or functionalized derivative of 3-oxapentandiaminde-type ionophores. This invention further provides sensors for detecting target ions in a sample, comprising plasticizer-free molecularly imprinted polymers, wherein the polymers comprise polymerized units of methacrylate monomers having pendent alkyl groups of different length and a functionalized ionophore of said ion. In particular, a magnesium ion sensor comprising a functionalized derivative of a 3-oxapentandiaminde-type calcium ion-selective ionophore is provided.

Abstract: Provided are a method for easily detecting phosphorylated peptides, namely, proteins, in samples derived from living organisms or the like, a method for selectively adsorbing the phosphorylated peptides, and compounds that are highly coordinated to the phosphorylated peptides and usable in the methods. The complex compound is represented by the formula: wherein X is a linker moiety, and Y is a labeling group. The compound (I) is highly coordinated to a phosphorylated peptide, and has a labeling group. Accordingly, with use of the compound (I), the phosphorylated peptide can be easily identified.

Abstract: The present invention is directed, in part, to fluorescein-based ligands for detection of metal ions, and methods of making and using the same.

Abstract: A method and an apparatus which continuously separate and measure mercury in an exhaust gas in accordance with each chemical conformation and display a measurement result in real time. According to the method and the apparatus, water-soluble mercury in a gas is absorbed into an absorption solution (7), the gas and the absorption solution (7) are then separated from each other, the water-soluble mercury in the absorption solution (7) is reduced to be converted into gaseous metal mercury and led to an analyzer (20), and metal mercury in the gas which is not absorbed into the absorption solution (7) is led to an analyzer (22) in the form of gas. As a result, the water-soluble mercury and the non-water-soluble mercury contained in the gas can be captured and measured/analyzed in respective measurement systems in accordance with each chemical conformation. In addition, a concentration of the metal mercury and that of the water-soluble mercury in the gas can be continuously monitored in real time.

Abstract: The present invention relates to a method for measuring the content of Lanthanides dissolved in uranium oxide, wherein the Lanthanides content in the nuclear fuel pellet is measured using the thermo gravimetric analysis which measures the weight variation caused by the oxidation and heat treatment of the nuclear fuel pellet. This method provides an advantage in that the Lanthanide content can be measured using relatively simple equipments such as an electric furnace and a balance.

Abstract: The invention concerns scintillation proximity assays performed in multiwell plates where a charge coupled device is used to image the wells. Conventional phosphors emit blue light (350–450 nm) which is absorbed by yellow or brown assay components. This problem is addressed by the use of phosphors that emit radiation of longer wavelength (480–900 nm).

Abstract: The invention relates to indicator molecules for detecting the presence or concentration of an analyte in a medium, such as a liquid, and to methods for achieving such detection. More particularly, the invention relates to copolymer macromolecules containing relatively hydrophobic indicator component monomers, and hydrophilic monomers, such that the macromolecule is capable of use in an aqueous environment.

Abstract: The present invention relates to nanocrystals consisting of a homogeneous ternary or quaternary alloy having the composition M11-xM2xA and M11-xM2xAyB1-y, respectively, a process for its production, as well as to uses of such nanocrystals such as as short wavelength light-emitting devices, and in the detection of analytes, in particular biomolecules.

Abstract: A method and system are provided for creating a mercury halide standard, and for using that standard to test a mercury analyzer system. Gaseous elemental mercury of a known concentration from a gas cylinder and a halogen donor are fed to a reaction chamber where they react to form mercury halide. The mercury halide is fed to a mercury analyzer system where it is converted to reform gaseous elemental mercury which is then measured by a mercury analyzer. By comparing either the amount of elemental mercury supplied to the reaction chamber or the amount of mercury halide formed in the reaction chamber with the amount of elemental mercury converted from the mercury halide, the ability of the mercury analyzer system to convert mercury halide to gaseous elemental mercury can be evaluated.

Abstract: This invention relates to a metal ion specific capacitance sensor with exceptional sensitivity and wide operating range. It is versatile because different kinds of recognition elements can be immobilized directly in a self-assembling monolayer substantially completely covering the surface of the measuring noble metal electrode. The electrode then becomes selective to those metal ions in the solution that show affinity to the recognition element on the surface. Compared to previously described electrochemical sensors, the sensor according to the present invention shows many orders of magnitude better sensitivity because of the unique measuring principle.

Abstract: The present invention relates to sensors for the determination of organometallic compounds comprising the following elements: a) an inorganic, organic or polymeric support, insoluble in the sample to analyze b) an indicator which reversibly reacts with the organometallic compound and whose reaction product gives a characteristic absorption, reflection or emission band in the range 150-15000 nm and which is physically trapped, adsorbed, absorbed, dissolved or chemically linked in an electrostatic or covalent way to the support c) an optical sensor which measures the absorption, reflection or emission of the functionalized support at the characteristic wavelength and transforms it in the concentration of the organometallic present in the solution. Also described is the method for the determination of the concentration of organometallic compounds by the on line use of a sensor as above defined.

Abstract: A method and system are provided for creating a mercury halide standard, and for using that standard to test a mercury analyzer system. Gaseous elemental mercury of a known concentration from a gas cylinder and a halogen donor are fed to a reaction chamber where they react to form mercury halide. The mercury halide is fed to a mercury analyzer system where it is converted to reform gaseous elemental mercury which is then measured by a mercury analyzer. By comparing either the amount of elemental mercury supplied to the reaction chamber or the amount of mercury halide formed in the reaction chamber with the amount of elemental mercury converted from the mercury halide, the ability of the mercury analyzer system to convert mercury halide to gaseous elemental mercury can be evaluated.

Abstract: A self-diagnostic test, a self-diagnostic test apparatus, and method of manufacturing a self-diagnostic test for screening for elemental mineral imbalances in a patient utilizing an analysis of the reaction of mineral specific reagents to a sample from a patient are provided. In one embodiment, the invention is directed to a test for those elements that occur naturally in the body. In such an embodiment, the invention may test for those elements that comprise about 0.001% of the body weight or less (microtrace), those elements that comprise about 4% of the body weight or less (trace), those elements that comprise up to 96% of the body weight (mass), or any combination of the above.

Abstract: Tandem mass spectrometry (MS/MS) has been used extensively for solving various biological problems, such as structure identification of oligopeptides, nucleic acids, and nucleotides. One of the main advances of MS/MS is that it lends itself to the quantitative and qualitative analysis of certain analytes and complex mixtures with little or no clean up. Quantitative analysis of amino acids and acylcarnitines using tandem mass spectrometry used to screen neonatal dried blood spot samples for disorders in the metabolism of amino acids, organic acids and fatty acids is an emerging technology. The method described herein extends the use of tandem mass spectrometry to the analysis of &agr;-hydroxyketones and hexose phosphates from blood spots for newborn screening. Thus, according to this invention one test can be used to screen a number of neonatal disorders and diseases.

Abstract: The invention relates to indicator molecules for detecting the presence or concentration of an analyte in a medium, such as a liquid, and to methods for achieving such detection. More particularly, the invention relates to copolymer macromolecules containing relatively hydrophobic indicator component monomers, and hydrophilic monomers, such that the macromolecule is capable of use in an aqueous environment.

Abstract: The present invention provides methods and systems that uses transgenic zebrafish with an easily assessable reporter gene under the control of pollutant-inducible DNA response elements. Transgenic zebrafish, carrying pollution-inducible response elements, are placed in the water to be tested, and the contaminants become bioconcentrated (generally 1,000- to 40,000-fold, relative to the water) in the tissues of the fish thereby activating specific response elements, which up-regulate the LUC reporter gene. Fish are then removed from the test water and placed immediately in a luminometer cuvette and incubated with luciferin. Luciferin is rapidly taken up into the tissues of the fish, oxidized by luciferase, and light is produced. The luminescence is proportional to the environmental concentration of the pollutant (to which the fish had been exposed), which drives the expression of the LUC gene by means of the various DNA motifs. The luminescence is quantitated in the luminometer.

Abstract: Zn(II) is selectively detected in a sample by contacting the sample with a tripodal ligand with a piperidine or quinuclidine scaffold, one of which acts as a zinc sensor, in which the rigidity of the ligand scaffold is increased. The rigidity of the ligand scaffold can be increased by adding aromatic groups or cyclic hydrocarbon groups. Examples of aromatic groups include naphthalene and the like. Examples of cyclic groups include nitrogen-substituted cyclohexane and cyclohexene such as piperidine.

Abstract: A test for determining the presence of multi-valent metal contaminants, such as arsenic, mercury and chromium, when present in certain valence states and a system for removal of these contaminants from water. Multi-valent metal salts, for example, Cr+6, which are highly toxic, can be detected and potentially removed from water through a redox reaction by reaction with iron or cobalt salts to obtain a reductive elimination of the Cr+6 by conversion to Cr+3. The determination may be in the form of a test, such that a tableted composition can be introduced into water for reduction of a metal salt, such as Cr+6 to Cr+3 in order to provide a visual indication thereof, The system for the conversion of Cr+6 or other reducible metal salts to other lower valence states having less toxicity would rely upon introduction of a metal salt in the form of a reducing agent which would be introduced into the water allowing for a reduction/oxidation action to take place.

Abstract: A particulate separation system for accurate measurement of vapor-phase mercury in a flue gas stream uses an inertial gas sampling filter in which the skin temperature of the filter element is controlled to allow vapor-phase mercury measurements while minimizing measurement artifacts caused by: (1) mercury thermally desorbing off particulates into the gas stream; and (2) mercury being removed from the vapor phase by collection on particulate matter at the gas/particle separation interface.

Abstract: A portable instrument for automatic collection and analysis of airborne lead concentrations in ambient air environments. Its improvements to the art of airborne lead detection and analysis permit the apparatus to analyze samples previously collected by personal monitors, for example, worn on the clothing of personnel working in contaminated sites. The apparatus also brings to the art of lead analysis a method of contaminant collection that ensures a greater capture efficiency of airborne contaminants, thereby increasing the accuracy of the instrument and its measurement capabilities while providing near real-time analysis and measurement in a portable self-contained battery-powered device. The apparatus also offers a remarkable improvement in the reduction of wastes incurred in the collection and analysis of airborne lead contaminants which is a novel method of concentrating the samples and recycling the analysis media used to concentrate and solubilize lead contaminants.

Abstract: Methods of analyzing mercury containing samples for inorganic and organomercurial complexes are disclosed. The methods are highly sensitive and are especially suited to samples containing significant amounts of organic matter. Kits and devices for mercury analysis are also disclosed.

Abstract: A reagent and kit are disclosed for determining if a patient is hypercoagulable, hypocoagulable or normal. The test involves providing a test sample from the patient and initiating coagulation in the sample in the presence of an activator, which is added to the sample in an amount which will result in intrinsic tenase-dependent fibrin. Then the formation of the intrinsic tenase-dependent fibrin polymerization is monitored over time so as to derive a time-dependent profile, with the results of the fibrin polymerization monitoring determining whether the patient is hypercoagulable, normal or hypocoagulable. The coagulation activator is added in an amount that triggers a thrombin explosion that is dependent on the propagation phase and amplification pathways. In this way, a single assay can assess the hemostatic potential of a sample.

Abstract: A compound represented by general formula (IA) or a salt thereof useful as a fluorescent probe for zinc: wherein R1 and R2 represent a hydrogen atom or a group represented by formula (A), wherein X1, X2, X3, and X4 represent a hydrogen atom, an alkyl group, a 2-pyridylmethyl group, or a protective group for an amino group, and m and n represent 0 or 1 provided that R1 and R2 do not simultaneously represent hydrogen atoms; R3 and R4 represent a hydrogen atom or a halogen atom; and R5 and R6 represent a hydrogen atom, an alkylcarbonyl group, or an alkylcarbonyloxymethyl group, and R7 represents a hydrogen atom or an alkyl group.

Abstract: A fluorescent probe is applied to bind to a metal oxide on a substrate and the substrate is exposed to an ultraviolet light to identify the metal oxide. A chemical cleaning or stripping solution is selected by combinatorial high throughput screening (CHTS). In the method, an array of regions is defined on a substrate, a candidate cleaning/stripping solution is deposited onto the regions to effect cleaning/stripping of the regions; a fluorescent probe is applied to bind to a metal oxide on the substrate; the substrate is exposed to an ultraviolet light to identify the metal oxide and a product of the cleaning/stripping is evaluated according to the identified metal oxide. An activated metal substrate composition is provided that comprises a metal substrate with a contaminant metal oxide coating and a fluorescent activator bound to the substrate by reaction with the metal oxide coating.

Abstract: A method for determining the amount of metal amino acid chelate present in various chelate compositions. FT-IR is used to determine the amount of free amino acid present. The bound amino acids may be in the form of an amino acid complex or an amino acid chelate. A total metal analysis and measurement of ligand quantity is then performed, from which the percent of metal amino acid chelate in the sample is calculated.

Abstract: The present invention relates to a method and a device for detecting mercury in a sample solution, the mercury being contained in the sample solution in cationic form and as an organic compound, by means of a detection device comprising an atomic spectroscope provided with a measuring cell which is suitable for detecting mercury gas, a noble-metal concentrator for concentrating the mercury and a control unit, the mercury being converted into the gas phase and being adapted to be concentrated by means of the noble-metal concentrator. The method according to the present invention is characterized in that the control unit controls the supply of the mercury gas to the measuring cell in such a way that, in addition to the measurement of the concentrated mercury, mercury gas of the same sample, which has not been subjected to the concentration step, is introduced in the measuring cell for the purpose of measurement.

Abstract: A method of assaying metal proteinate samples to determine compliance with European Union standard definitions for such products.

Abstract: A method is provided for producing a visual image of ions in a thin frozen tissue sample by placing the tissue sample in contact with a thin membrane having a water soluble gel or hydrophobic plastic thin film which has incorporated in or placed on its surface a fluorescent or color ion indicator which fluoresces or changes color under light of a particular wavelength. The thin frozen tissue sample is then subjected to light having a wavelength to cause the ion indicator to fluoresce or change color.

Abstract: A portable instrument for automatic collection and analysis of airborne lead concentrations in ambient air environments. Its improvements to the art of airborne lead detection and analysis permit the apparatus to analyze samples previously collected by personal monitors, for example, worn on the clothing of personnel working in contaminated sites. The apparatus also brings to the art of lead analysis a method of contaminant collection that ensures a greater capture efficiency of airborne contaminants, thereby increasing the accuracy of the instrument and its measurement capabilities while providing near real-time analysis and measurement in a portable self-contained battery-powered device. The apparatus also offers a remarkable improvement in the reduction of wastes incurred in the collection and analysis of airborne lead contaminants which is a novel method of concentrating the samples and recycling the analysis media used to concentrate and solubilize lead contaminants.

Abstract: Disclosed is a method of performing assay to a fluorite sample, which includes a first step for dissolving a fluorite sample, containing Ca and F, by use of a solvent, a second step for removing Ca and F from an obtained solution, and a third step for assaying, through ICP-MS, the solution having Ca and F removed therefrom. Also disclosed is a method of producing a fluorite crystal and an exposure apparatus using such fluorite crystal, and a device manufacturing method using such exposure apparatus. The assaying method of the present invention ensures assay and evaluation of a fluorite sample, of sensitivity several times higher than conventional methods.

Abstract: The present invention is a colorimetric method, and kit therefore, for determining the level of elemental contaminants in a water source. In a preferred embodiment, the method comprises: (a) sampling a discrete amount of water to be tested, (b) contacting the water sample with a solid material having high specificity for reversibly binding to, or complexing with, the element to be detected, (c) separating the element-containing solid from the water sample, (d) eluting the element from the element-containing solid with an eluting solution, (e) adding a cation-containing solution capable of freeing the element to be detected from the eluting compound, (f) adding a buffer, (g) adding a colorimetric material capable of indicating the amount of metal present in the solution, (h) adding an oxidation-fixing reagent, and (i) estimating the amount of metal in the sample by comparing the solution to provided color standards.

Abstract: A method and apparatus are provided for collecting a sample gaseous mercury, to differentiate between different gaseous mercury components. A quartz denuder module is provided having a coated extended surface for adsorbing reactive gaseous mercury. After collection of a sample, the coating it is heated to desorb the mercury as elemental gaseous mercury, which can then be detected and measured in conventional analyzer. During the sampling phase, as reactive gaseous mercury is removed from the sample flow in the denuder, the sample can then be passed to the analyzer for detection of elemental gaseous mercury. Where particulate mercury may be present, a filter trap can be provided downstream from the denuder. Separate steps can be provided for heating and pyrolysis of particulate mercury, for separate measurement in the analyzer. The pump module can be configured to a past the gas sample through scrubbers, to generate zero air as a flushing gas.

Abstract: A system and method for controlling a microwave heated chemical process is disclosed. Time varying concentrations of some chemical substances within fumes in the microwave oven are monitored to detect concentration variations for which responses are known. Responses to the detected variations are initiated to control the chemical process without terminating the process. Examples of responses include varying microwave radiation energy, initiating safety systems, increased venting of the microwave oven, and so forth.

Abstract: A test for determining the presence of multi-valent metal contaminants, such as arsenic, mercury and chromium, when present in certain valence states and a system for removal of these contaminants from water. Multi-valent metal salts, for example, Cr+6, which are highly toxic, can be detected and potentially removed from water through a redox reaction by reaction with iron or cobalt salts to obtain a reductive elimination of the Cr+6 by conversion to Cr+3. The determination may be in the form of a test, such that a tableted composition can be introduced into water for reduction of a metal salt, such as Cr+6 to Cr+3 in order to provide a visual indication thereof. The system for the conversion of Cr+6 or other reducible metal salts to other lower valence states having less toxicity would rely upon introduction of a metal salt in the form of a reducing agent which would be introduced into the water allowing for a reduction/oxidation action to take place.

Abstract: The present invention refers to a method for atomic absorption spectroscopy of an analyte which is contained in a sample to be analyzed and which is converted into free atoms in an absorption volume of an atomizer, said method comprising the steps of (a) position- and time-dependent measuring of the atomic absorption over the cross-section of the absorption volume and (b) simultaneous determination of surface temperatures of the atomizer.

Abstract: Homogeneous fluorescence polarization (anisotropy) assays for detecting and quantifying metal ions in solution, based the metal-dependent binding of a fluorescent ligand to an unlabeled macromolecule, or the binding of a metal ion to a fluorescent labeled macromolecule. The metal-dependent binding of a fluorescent ligand to an unlabeled macromolecule (metallo-macromolecule) effects a measurable change in anisotropy as will the binding of metal ions to a fluorescent labeled macromolecule. Binding of the fluorescent ligand to the unlabeled macromolecule is metal dependent with the change in anisotropy being proportional to the concentration of bound metal ions. No binding of the fluorescent ligand to the macromolecule occurs in the absence of metal ions. Conversely, if the fluorescent label is first conjugated to a metallo-macromolecule and the metallo-macromolecule is subsequently stripped of its metal ion, it may then be used to transduce the binding of metal ions.

Abstract: The present invention provides a method of screening for risk of pancreatic cancer in a subject, wherein the presence or absence of increased levels of cadmium in the subject is detected and wherein the presence of increased levels of cadmium in the subject indicates that the subject is at increased risk of pancreatic cancer.

Abstract: A method for evaluating ratios of metallic impurities in lithographic materials is disclosed. The method comprises: separating said metal from said lithographic material by microwave heating; then adding said metal to an acid to form a solution; and finally analyzing said solution by a instrument to measure ratio of said metal.

Abstract: The present invention relates to a method and a device for detecting mercury in a sample solution, the mercury being contained in the sample solution in cationic form and as an organic compound, by means of a detection device comprising an atomic spectroscope provided with a measuring cell which is suitable for detecting mercury gas, a noble-metal concentrator for concentrating the mercury and a control unit, the mercury being converted into the gas phase and being adapted to be concentrated by means of the noble-metal concentrator. The method according to the present invention is characterized in that the control unit controls the supply of the mercury gas to the measuring cell in such a way that, in addition to the measurement of the concentrated mercury, mercury gas of the same sample, which has not been subjected to the concentration step, is introduced in the measuring cell for the purpose of measurement.

Abstract: A device is provided for capturing ionic metal species (e.g., Cd, Cu, Ni, Pb and Zn) dissolved in water. The device includes a nonporous polymeric tube for capturing the dissolved ionic metal species. The tube is formed from a membrane having transient cavities therein having an average pore size greater than 10 Å. A sequestration medium, contained within the tube, transforms the ionic metal species captured by the tube into a complexed, non-mobile metal species. The sequestration medium is capable of diffusing through the nonporous polymeric membrane at a controlled rate to form the complexed, non-mobile species and can comprise a mixture and metal complexing agent (e.g., a quinoline) and a long chain organic acid (e.g., oleic acid).

Abstract: Homogeneous fluorescence polarization (anisotropy) assays for detecting and quantifying metal ions in solution, based the metal-dependent binding of a fluorescent ligand to an unlabeled macromolecule, or the binding of a metal ion to a fluorescent labeled macromolecule. The metal-dependent binding of a fluorescent ligand to an unlabeled macromolecule (metallo-macromolecule) effects a measurable change in anisotropy as will the binding of metal ions to a fluorescent labeled macromolecule. Binding of the fluorescent ligand to the unlabeled macromolecule is metal dependent with the change in anisotropy being proportional to the concentration of bound metal ions. No binding of the fluorescent ligand to the macromolecule occurs in the absence of metal ions. Conversely, if the fluorescent label is first conjugated to a metallo-macromolecule and the metallo-macromolecule is subsequently stripped of its metal ion, it may then be used to transduce the binding of metal ions.

Abstract: A method and apparatus are provided for collecting a sample gaseous mercury, to differentiate between different gaseous mercury components. A quartz denuder module is provided having a coated extended surface for adsorbing reactive gaseous mercury. After collection of a sample, the coating it is heated to desorb the mercury as elemental gaseous mercury, which can then be detected and measured in conventional analyzer. During the sampling phase, as reactive gaseous mercury is removed from the sample flow in the denuder, the sample can then be passed to the analyzer for detection of elemental gaseous mercury. Where particulate mercury may be present, a filter trap can be provided downstream from the denuder. Separate steps can be provided for heating and pyrolysis of particulate mercury, for separate measurement in the analyzer. The pump module can be configured to a past the gas sample through scrubbers, to generate zero air as a flushing gas.

Abstract: Method and reagent for the determination of iron in a biological sample in which the bound iron is released, the released iron is reduced to Fe2+, a color reagent solution is added and the color complex that is formed is measured photometrically, characterized in that a water-soluble EDTA-complexing compound in particular an indium and/or scandium salt is added to the sample.

Abstract: This invention relates to diagnostic methods and test kits for rapidly assessing the coagulant activity of blood by measuring the rate of blood clotting in the presence of certain metal ions. A modulator of coagulation may also be included. The coagulation activity of the blood of the patient in the presence of metal ions and an optional modulator is an indication of the presence or development of certain pathological conditions relating to the procoagulant activity of blood, including platelet function. The effect of metal ions on the viscosity of blood is also diagnostically useful.