Plural Nitrogen In The Same Ring (e.g., Barbituates, Creatinine, Etc.) Patents (Class 436/98)
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Patent number: 5543330Abstract: An improved method for diagnosing myofibrogranuloma (MFG) in walleye without harming the fish is described. Blood samples are drawn from walleye and creatinine and calcium levels are then determined along with the sex and length of the fish. These data are then analyzed and compared to data from healthy walleye using discriminant analysis. The process is effective for identifying approximately 88% of MFG-positive walleye.Type: GrantFiled: September 22, 1995Date of Patent: August 6, 1996Assignee: Center for Innovation & Business Development FoundationInventors: Harry L. Holloway, Jr., Craig A. Shoemaker
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Patent number: 5527708Abstract: A quantitative assay for creatinine has been developed in which creatinine is reacted with 3,5-dinitrobenzoate under alkaline reaction conditions to produce a fluorophore product which has excitation and emission maxima near 410 nm and 475 nm, respectively. Alternatively, 3,5-dinitrobenzoyl chloride or methyl-3,5-dinitrobenzoate may be employed as reagents. The chemical reactions proceed in the presence of Group IA bases and/or quaternary alkyl ammonium hydroxides under aqueous, nonaqueous and mixed solvent conditions. Detection limits for creatinine are well below one micromole per liter. Conversely, the reactivity of creatinine and/or base with 3,5-dinitrobenzoate may be employed to quantify 3,5-dinitrobenzoate by fluorescence intensity measurements. The chemical assay is readily adaptable to reagent pad and dry-layered coating technologies employing fluorescence detection/quantitation.Type: GrantFiled: May 2, 1995Date of Patent: June 18, 1996Inventor: Karl G. Blass
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Patent number: 5516699Abstract: A method of diagnosing severity of periodontal disease or peri-implant disease in a human patient, involving a) obtaining a sample of tissue or gingival crevicular or other fluid from the mouth of said patient, or a blood or urine sample from the patient, and b) measuring a pyridinoline crosslinked compound in the sample as a measure of severity of disease.Type: GrantFiled: February 16, 1994Date of Patent: May 14, 1996Assignees: Institute of Molecular Biology, Inc., President and Fellows of Harvard CollegeInventors: William V. Giannobile, Ray C. Williams, Samuel E. Lynch
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Patent number: 5500372Abstract: In a method of determining creatinine clearance for detecting and monitoring renal dysfunction, spot samples of urine and blood from a person are obtained. Specific gravity and creatinine concentration of the urine sample are measured. Creatinine concentration of plasma of the blood sample is also measured. Creatinine clearance is calculated as a function of the measured urine specific gravity, the measured urine creatinine concentration and the measured plasma creatinine concentration.Type: GrantFiled: July 25, 1994Date of Patent: March 19, 1996Assignee: Private Clinic Laboratories Inc.Inventor: Michael Kell
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Patent number: 5480807Abstract: The present invention is directed to use of an imidazo [4,5b] pyridinium molecule composed of a lysine and an arginine residue crosslinked with a pentose sugar for assessing the biological age of a tissue.Type: GrantFiled: October 28, 1994Date of Patent: January 2, 1996Assignee: Case Western Reserve UniversityInventors: Vincent M. Monnier, David R. Sell
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Patent number: 5464777Abstract: A method for the determination of protein and creatinine in urine. An organic acid, in the form of its lithium salt, is dried onto a suitable substrate in the second reaction zone of a reaction vessel having a first reaction zone for the immunometric determination of the protein and a second reaction zone for the colorimetric determination of creatinine. After completion of the immunometric determination of the protein the dried organic salt is contacted with the urine containing creatinine resulting in solubilization of the organic acid salt as creatinine reactive reagent thereby facilitating the colorimetric determination of the creatinine.Type: GrantFiled: September 26, 1994Date of Patent: November 7, 1995Assignee: Miles Inc.Inventor: Kin-Fai Yip
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Patent number: 5420275Abstract: An assay method which comprises utilizing chemiluminescence of a pyridopyridazine compound of the formula ##STR1## wherein R.sub.1 is a hydrocarbon group or a heterocyclic group each of which may be substituted and R.sub.2 is hydroxy group, thiol group, amino group or a monosubstituted amino group, and when R.sub.2 is a monosubstituted amino group, R.sub.2 may be taken together with R.sub.1 to form the ring; R.sub.3 is hydrogen atom, a hydroxy group which may be substituted, an amino group which may be substituted, a thiol group which may be substituted, a halogen atom, a heterocyclic group, nitro group, cyano group, carboxyl group which may be esterified or amidated, azido group, sulfo group or an organic sulfonyl group, provided that when R.sub.1 is an aliphalic group, R.sub.3 is not hydrogen atom; and X is oxygen atom or sulfur atom/or a salt thereof; and a novel compound of the formula (I) wherein the symbols are as defined above with proviso that R.sub.3 is hydrogen atom, R.sub.Type: GrantFiled: November 2, 1993Date of Patent: May 30, 1995Assignee: Takeda Chemical Industries, Ltd.Inventors: Hirotomo Masuya, Yoshio Aramaki
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Patent number: 5385847Abstract: Disclosed is a method for the determination of urinary protein and creatinine in a single reaction vessel using a continuous process. The method involves adjusting the pH to a level suitable for carrying out an immuno assay for the protein and making such a determination by an immuno agglutination technique. Raising the pH to a level suitable for determining the creatinine quickly dissipates the cloudiness caused by the agglutination reaction, so that the creatinine determination can be carried out without delay.Type: GrantFiled: December 2, 1993Date of Patent: January 31, 1995Assignee: Miles Inc.Inventors: Kin F. Yip, Amy H. Chu, Brenda Tudor
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Patent number: 5374561Abstract: The present invention is a method for the detection of creatinine in an aqueous solution, particularly urine, which involves contacting the solution with a soluble cupric salt, a hydroperoxide and an oxidizable indicator which provides a detectable response in the presence of oxygen free radicals and a pseudoperoxidase. The invention is predicated on the discovery that cupric ions form a complex with creatinine which complex exhibits peroxidase like activity.Type: GrantFiled: October 25, 1993Date of Patent: December 20, 1994Assignee: Miles Inc.Inventor: Michael J. Pugia
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Patent number: 5324835Abstract: Compounds useful as luminescent labelling reagents or reagents for determining hydrogen peroxide of the following formula: ##STR1## Wherein R is selected from the group consisting of hydrogen, amino, carboxyl, thiol, n-propyl, isopropyl, n-butyl, sec-butyl, benzyl, carboxyethyl, tert-butyl, and--(CH.sub.2).sub.n1 CH.sub.3 wherein n.sub. is 0 or an integer of 1-15;--(CH.sub.2).sub.n2 COOH wherein n.sub.2 is 0 or an integer of 1-5;--CH.sub.2 SH; --CH.sub.2 X.sub.1 wherein X is Br, Cl or F;--(CH.sub.2).sub.n3 NH.sub.2 wherein n.sub.3 is an integer of 1-5; ##STR2## wherein n.sub.4 is 0 or 1 and n.sub.5 is 0 or ##STR3## wherein n.sub.6 is 0 or 1 and X.sub.2 is H, Br, Cl, F or I; and --(CH.sub.2).sub.n7 R.sub.1 wherein n.sub.7 is 0 or an integer of 1-5 and R.sub.1 is selected from COCl, CON.sub.3, CONHNH.sub.Type: GrantFiled: August 7, 1992Date of Patent: June 28, 1994Assignee: Biosensor Laboratories Co., Ltd.Inventor: Masatoshi Yamaguchi
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Patent number: 5290702Abstract: Chromogenic solvent detectors are used to detect and map the presence of organic solvent-containing materials on a surface. The detector need only be applied to the surface to be tested. When removed, the dark areas on the detector correspond to those areas of the surface where an organic solvent-containing material was present. This method can be used to test the release properties of a release film.Type: GrantFiled: March 19, 1993Date of Patent: March 1, 1994Assignee: Valence Technology, Inc.Inventor: On-Kok Chang
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Patent number: 5284748Abstract: A method is disclosed for detecting the occurrence of a binding or complex-forming reaction between specific substances by utilizing the binding reaction to modify an electrical circuit, and then measuring a change in the electrical state of the circuit. A diagnostic element useful in such a method includes a layer of a biogenic substance, such as an antigen, coated onto a non-conductive base between a pair of electrical conductors superposed on the base. Antibodies which react with the antigen are treated so that they become bound to particles. The particles having antibody bound thereto are then added to is the antigen lay e base and allowed to react therewith. The particles are thereby bound to the base due to the binding reaction between the antigen and antibody to thereby form aggregates of electrically conductive particles which modify the circuit. The particles are then selectively coated with a conductive substance.Type: GrantFiled: April 10, 1992Date of Patent: February 8, 1994Assignee: Immunotronics, Inc.Inventors: Susan J. Mroczkowski, Kenneth A. Siegesmund, Donald E. Yorde
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Patent number: 5278074Abstract: The concentration of an aromatic azole corrosion inhibitor in the water of an aqueous system is monitored by a fluorometric method which is based upon the measurement of the fluorescence intensity of the aromatic azole corrosion inhibitor itself, and the precise determination of concentration permits control of the dosage of such aromatic azole corrosion inhibitor.Type: GrantFiled: April 22, 1992Date of Patent: January 11, 1994Assignee: Nalco Chemical CompanyInventors: Narasimha M. Rao, Frank F.-Y. Lu, John E. Hoots
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Patent number: 5268302Abstract: A method and apparatus for selective, high speed detection of vapors of specific gas-chromatographically-separable compounds. In the disclosed method separate analyses are performed on two portions of a gas sample formed by flash-heating trapped vapors to successively higher temperatures while flowing hydrogen carrier gas over coatings in/on which the vapors are held. Within a total time interval of about twenty seconds 1) two sample portions are formed, 2) each portion is rapidly separated in two series-connected, high speed, temperature-programmed gas chromatographs, and 3) specific compounds are identified by detection of NO gas formed during an oxidative pyrolysis of each separated portion. One application of the described method and apparatus is the rapid, selective, and sensitive detection of nitrogen-containing compounds such as the drugs methamphetamine, cocaine, and heroin.Type: GrantFiled: March 22, 1993Date of Patent: December 7, 1993Assignee: Thermedics Inc.Inventors: David P. Rounbehler, David P. Lieb
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Patent number: 5212309Abstract: Chemiluminescenct 3-aminobenzene-1,2,4,5-tetracarboxylic dihydrazides.Type: GrantFiled: February 3, 1992Date of Patent: May 18, 1993Assignee: BASF AktiengesellschaftInventors: Ernst Schefczik, Klaus Huemke
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Patent number: 5064767Abstract: Polypeptides are sequenced from the carboxyl terminus by treating the peptide with an iodoxybenzene, followed by removal of the excess reagent and reaction with 2-aminothiophenol at an elevated temperature. The resulting benzthiazolidine is then analyzed to determine the C-terminal amino acid which has been cleaved. A kit which contains the iodoxybenzene and 2-aminothiophenol reagents is separate containers is also disclosed.Type: GrantFiled: June 21, 1990Date of Patent: November 12, 1991Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Dean A. Le, Kazuhiko Tatemoto
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Patent number: 4992296Abstract: A method of making drug abuse test papers wherein bibulous paper carriers are impregnated with specific test chemicals, (bismuth subnitrate, potassium iodide, acetic acid, and platinum salt), in two coatings and dried after each coating under specific temperature conditions. The test chemicals are provided for the detection of the drug abuse compounds of amphetamine, cocaine, marijuana, and narcotics contained in low concentrations in animal or human urine. Additionally described is a means of preserving urine samples using esters of parahydroxybenzoic acid.Type: GrantFiled: February 24, 1989Date of Patent: February 12, 1991Inventor: Jacob J. Gibson
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Patent number: 4950611Abstract: A cold stable liquid reagent system suitable for use in a Jaffe method for the determination of creatinine comprises:(a) a homogeneous liquid picric acid component comprising picric acid in an amount of no more than about 35 mM, such that the picric acid component remains visibly homogeneous at a temperature between about 2 to about 8.degree. C.Type: GrantFiled: June 26, 1987Date of Patent: August 21, 1990Assignee: Beckman InstrumentsInventor: Charles B. Seaton
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Patent number: 4914036Abstract: A method for analyzing vanillylmandelic acid, homovanillic acid and creatinine by fast liquid chromatography, which comprises a step of introducing a test solution containing vanillylmandelic acid, homovanillic acid and creatinine to a cation exchange resin column and separating creatinine from vanillylmandelic acid and homovanillic acid; a step of separating by a separation column vanillylmandelic acid and homovanillic acid in the test solution from which creatinine has been separated, and a step of detecting each component.Type: GrantFiled: March 2, 1988Date of Patent: April 3, 1990Assignee: Tosoh CorporationInventors: Seiji Kawaguchi, Hiroaki Takahashi, Yuji Saito
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Patent number: 4902627Abstract: A method of analyzing a sample for selected molecules which consists of reacting the molecule with a reagent that produces a charged derivative which can be desorbed and analyzed using Secondary Ion Mass Spectrometry (SIMS).Type: GrantFiled: February 5, 1985Date of Patent: February 20, 1990Assignee: The United States of America as represented by the Secretary of the NavyInventor: David Kidwell
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Patent number: 4882285Abstract: Pool or spa water can be monitored for dimethyl hydantoin content, as a residue of disinfection with halogenated hydantoin, by determining the quantity of base required to adjust the pH to 9.3. The pH may be judged by a suitable indicator for example thymol phthalein or thymol blue. The method can be applied either titrimetrically, or by comparison colorimetrically with standard solutions and may be modified for use in relation to other halogenated hydantoins. The method may be applied to the testing of pools for compliance with an advisory dimethyl hydantoin content limit and may be embodied in a kit for easy poolside use.Type: GrantFiled: February 22, 1989Date of Patent: November 21, 1989Assignee: Laporte Industries LimitedInventors: John W. Ogleby, Neville J. Charkley
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Patent number: 4837157Abstract: A plurality of analytes are analyzed by liquid chromatography in a liquid biological sample containing many components which can interfere with analysis by liquid chromatography. The sample, containing the analytes of interest, are passed to a first passage of a dialyzer which removes molecules in the sample which interfere with analysis. A diluent is passed through a second passage of the dialyzer in which the two liquids are separated from each other by a semipermeable membrane having holes large enough to permit the analytes of interest to pass into the diluent but holes small enough to prevent passage of sample components which interfere with analysis. The analyte-containing diluent is passed to a retaining element for temporarily retaining the analytes of interest by adsorption and/or partition. The analytes of interest are removed from the retaining element by a second diluent which is then passed through a chromatography column with a detector and the analytes are analyzed chromatographically.Type: GrantFiled: October 8, 1986Date of Patent: June 6, 1989Assignee: Coventry Health AuthorityInventors: David C. Turnell, John D. H. Cooper
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Patent number: 4818703Abstract: The alkaline picrate reagent solution employed in the Jaffe procedure for determining the presence and concentration of creatinine is stabilized for at least one year to permit long shelf life and improve the efficiency of creatinine testing. Stability is achieved by the addition of 5 to 20% by volume of a low molecular weight alcohol such as methanol.Type: GrantFiled: July 27, 1987Date of Patent: April 4, 1989Inventor: John M. Pizzolante
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Patent number: 4806487Abstract: A method for detecting basic narcotics or drugs in body fluids which comprises filtering body fluids through a cellulosic filtering means treated with a binding agent for the narcotics or drugs and the filtering means. The binding agent is a polycarboxylic acid. The narcotics or drugs are then treated with a subsequent reagent to produce a color reaction. A device for detecting basic narcotics or drugs in also disclosed which contains the filtering means treated with the binding agent so as to concentrate the narcotic or drug prior to reaction with a suitable indicator.Type: GrantFiled: May 29, 1987Date of Patent: February 21, 1989Assignee: Analytical Innovations, Inc.Inventors: Susan Akers, Raymond F. Akers, Jr.
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Patent number: 4806490Abstract: The invention relates to a procedure to establish the presence of allergen-containing housedust, as results when or after housedust mites occur, in which samples of dust present in buildings are thoroughly suspended in a aqueous-alcoholic alkali metal hydroxide solution and, with the resulting extract, a color reaction using an aromatic diazo compound is carried out, the color reaction being evaluated with respect to its occurrence as an indication of the pollution of the dust with housedust mite residues. According to a particular embodiment, a paper treated with the aromatic diazo compound (and where appropriate with starch) is used for carrying out the color reaction. A further aspect of the invention involves quantitatively determining the allergological activity resulting from housedust mites in housedust samples.Type: GrantFiled: February 19, 1987Date of Patent: February 21, 1989Assignee: Werner & Mertz GmbHInventors: Edelbert Bischoff, Wolfgang Schirmacher
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Patent number: 4752448Abstract: A diagnostic test paper detects abuse-type drugs in a test specimen of a biological fluid. A particular method of making the diagnostic test paper includes impregnating a bibulous carrier material with the dry residue of a reagent reactive to abuse-type drugs. A particular method is used for screening a biological fluid to determine the presence of abuse-type drugs therein. A test specimen applying area is located on the bibulous carrier material adjacent one side of a concentrated reagent zone having a first color. A particular method of application and a specific syringe device is used to apply a test specimen that has been prepared under very precise conditions. The test specimen moves from the particular applying area into the concentrated reagent zone to produce the specific results of detecting the presence of abuse-type drugs therein. A novel solvent composition is used to prepare the test specimen applied to the bibulous carrier material of the invention.Type: GrantFiled: December 17, 1986Date of Patent: June 21, 1988Assignee: Keystone Diagnostics, Inc.Inventors: Henry J. Wells, Robin L. Binder
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Patent number: 4743561Abstract: An improved method and reagents are disclosed for determining a ligand in an assay solution containing the ligand, a reagent system and a luminescent compounds, wherein the intensity of the light emitted by the assay solution is related to the change in the transmittive properties of the assay solution produced by the interaction of the ligand to be determined and a reagent system capable of producing a change in the transmittive properties of the assay solution in the presence of the ligand.Type: GrantFiled: March 5, 1985Date of Patent: May 10, 1988Assignee: Abbott LaboratoriesInventor: Mark R. Shaffar
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Patent number: 4725555Abstract: A system for determining zinc protoporphyrin levels in whole blood using hematofluorometry without the need for oxygenation of the hemoglobin by derivatizing the hemoglobin to form a new compound having essentially the same spectral properties as oxygenated hemoglobin. A preferred reagent includes cyanide as the active ingredient.Type: GrantFiled: October 22, 1986Date of Patent: February 16, 1988Assignee: Helena Laboratories CorporationInventors: Robert F. Labbe, Rebecca L. Rettmer
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Patent number: 4713346Abstract: A method of forming analyzable adducts in a mixture of compounds by contacting the mixture with a boron reagent having the formula of either ##STR1## where each X and Y is, independently, an alkyl group of 12 or fewer carbons or an aryl group of 6-20 carbons; or BZ.sub.3, where each Z is, independently, an alkyl group of 12 or fewer carbons, or an aryl group of 6-20 carbons.Type: GrantFiled: February 3, 1986Date of Patent: December 15, 1987Assignee: The Children's Medical Center CorporationInventors: Paul M. Gallop, Edward Henson, Rudolf Fluckiger
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Patent number: 4673467Abstract: A method is provided for depositing on a substrate a layer of fine-grained copper especially adapted for the making of masters for optical information storage discs, comprising the steps of placing a substrate in an electroplating bath comprising from about 180 to about 220 grams/liter of copper sulfate, from about 40 to about 80 grams/liter of sulfuric acid, from about 30 to about 60 ppm of chloride ion, from about 1.0 to about 15 grams/liter of a polyether having a molecular weight from about 4,000 to about 10,000, from about 1.0 to about 100 milligrams/liter of a sulfonated, sulfurized benzene compound, and from about 3.5 to about 30 milligrams/liter of 1-lower alkyl-2-mercapto imidazole; and passing electric current through the bath to deposit copper on the substrate. Also disclosed is a method of using high performance liquid chromatography techniques to quantitatively measure 1-lower alkyl-2-mercapto imidazole concentration and to control the same.Type: GrantFiled: September 2, 1986Date of Patent: June 16, 1987Assignee: CBS Inc.Inventor: Hanphire H. Nee
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Patent number: 4600696Abstract: A process and kit are provided for measuring chemical and physical stresses on the blood platelets of a human patient by measuring reduction in ATP (adenosine triphosphate) and ADP (adenosine diphosphate) concentrations while measuring increases in the ATP degradation products HYPX (hypoxanthine) and AMP (adenosine monophosphate). The process is useful for measuring a human patient's risk to internal stress such as reduction in the size of blood vessels, to response to various environmental changes as well as to a variety of treatments including drug treatment, risk of malignant hyperthermia in response to anesthetic administration or in response to vascular or organ transplant.Type: GrantFiled: June 22, 1984Date of Patent: July 15, 1986Inventor: Clive C. Solomons
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Patent number: 4550085Abstract: Histamine is detected or determined selectively in a sample by causing the sample to contact glass microfibers in such a quantitative proportion as will permit the histamine present to bind to the fibers. The amount of bound histamine may be determined by competitive determination in the presence of labelled histamine on the basis of a standard curve, or may be determined directly by conventional coupling reactions.The method is simple and particularly useful for allergy diagnostics because it exhibits good correlation with known fluorometric methods.Type: GrantFiled: September 8, 1981Date of Patent: October 29, 1985Inventors: Per S. Skov, Svend Norn, Bent Weeke
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Patent number: 4529708Abstract: An improved colorimetric assay method and reagent composition for the quantitative determination of creatinine in biological fluid specimens in the presence of protein, in which the specimen is reacted directly with an alkaline picrate reagent composition which contains an iron chelator, preferably ethylenediaminetetraacetic acid, thereby significantly suppressing interference from bilirubin and improving reagent stability.Type: GrantFiled: April 7, 1983Date of Patent: July 16, 1985Assignee: American Monitor CorporationInventor: Thomas W. Stephens
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Patent number: 4460695Abstract: A method for the separation of theophylline and caffeine contained in body fluids is provided. A macroreticular styrene-divinylbenzene copolymer resin activated by a protic solvent is utilized. The body fluid is eluted with water in an isocratic manner to afford a theophylline sample uncontaminated with caffeine. Such samples are useful in immunoassay procedures for the measurement of theophylline.Type: GrantFiled: December 24, 1981Date of Patent: July 17, 1984Assignee: E. I. DuPont de Nemours and CompanyInventors: Johanne C. Dickinson, William A. Frey
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Patent number: 4426451Abstract: A reaction vessel, test device, and method of detection or measurement are disclosed, featuring the use of at least two operatively connected zones formed by transport surfaces spaced apart throughout most of the zones a capillary distance. The zones are fluidly connected by meniscus control means effective to stop capillary flow of the liquid from one zone to the other, until an externally generated actuation pressure is applied.Type: GrantFiled: January 28, 1981Date of Patent: January 17, 1984Assignee: Eastman Kodak CompanyInventor: Richard L. Columbus
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Patent number: 4419452Abstract: A method for detecting fluorescent materials in liquid chromatography. Fluorescent materials are excited by chemical reaction with chemical reagents to yield light (chemiluminescence) and detected at high sensitivity. The detection apparatus is composed of a mixer for mixing a solution containing separated fluorescent materials and solutions of chemical reagents to afford chemiluminescence, a flow cell and a light receptive detection means for detecting the light thus produced.Type: GrantFiled: August 12, 1981Date of Patent: December 6, 1983Assignee: Yamanouchi Pharmaceutical Co., Ltd.Inventors: Kazuhiro Imai, Zenzo Tamura, Shin-ichiro Kobayashi
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Patent number: 4396718Abstract: This invention relates to use of adsorption resins and ion exchange resins to separate selected organic materials and quantify their concentration in an aqueous solution based upon comparison to a visual standard. This process can be carried out in a relatively short time period and does not require special electronic equipment. Therefore, it can be conveniently used as a field test method to determine concentration of organic materials in aqueous solutions. This invention is especially useful in quantifying the concentration of triazoles in industrial cooling water.Type: GrantFiled: October 28, 1980Date of Patent: August 2, 1983Assignee: The Sherwin-Williams CompanyInventor: Clare B. Proudfoot