By Measuring A Physical Property (e.g., Mass, Etc.) Patents (Class 506/12)
  • Patent number: 10809244
    Abstract: A method of analyzing molecules using a nanopore array including a plurality of cells included on a chip is disclosed. Nanopores are caused to be formed in at least a portion of the plurality of the cells. A first physical measurement of the nanopores is evaluated. It is determined whether to cause the molecules to interact with the nanopores. At least a portion of the nanopores is caused to interact with the molecules. A second physical measurement of the nanopores that indicates a property of the molecules is evaluated. It is determined whether to cause the nanopores to be reformed so that the cells may be reused to interact with additional molecules.
    Type: Grant
    Filed: May 18, 2018
    Date of Patent: October 20, 2020
    Assignee: Roche Sequencing Solutions, Inc.
    Inventors: Roger J. A. Chen, David J. Fullagar
  • Patent number: 10745762
    Abstract: The disclosed embodiments relate to method, system and apparatus for synthesizing a target polynucleotide within a droplet. In an exemplary embodiment, the disclosure provides a method of synthesizing a target polynucleotide. The method includes the steps of: contacting a polynucleotide-containing component from a sample with lysis reagents in a droplet, the lysis reagents comprising an enzyme having protease activity, wherein the droplet is encapsulated with an immiscible carrier fluid; moving the droplet into a collection reservoir; incubating the droplet in the collection reservoir for a first duration and then inactivating the enzyme having protease activity; adding to the droplet a nucleic acid synthesis reagent to form a nucleic acid synthesis droplet in the immiscible carrier fluid to form a nucleic acid droplet; and synthesizing the target polynucleotide within the nucleic acid synthesis droplet.
    Type: Grant
    Filed: February 19, 2019
    Date of Patent: August 18, 2020
    Assignee: The Regents of the University of California
    Inventors: Adam R. Abate, Dennis Jay Eastburn, Adam R. Sciambi
  • Patent number: 10741291
    Abstract: A computer-based genomic annotation system, including a database configured to store genomic data, non-transitory memory configured to store instructions, and at least one processor coupled with the memory, the processor configured to implement the instructions in order to implement an annotation pipeline and at least one module filtering or analysis of the genomic data.
    Type: Grant
    Filed: March 26, 2015
    Date of Patent: August 11, 2020
    Inventor: Ali Torkamani
  • Patent number: 10704965
    Abstract: A pressure sensing device that may include a first and second sensing elements that include one or more piezoresistive materials. The first sensing element has a first gradient. The second sensing element has a second gradient. The second gradient differs from the first gradient. The first and second gradients facilitate a determination of a magnitude of and/or a location of pressure applied on the first and second sensing element.
    Type: Grant
    Filed: December 9, 2015
    Date of Patent: July 7, 2020
    Inventors: Hossam Haick, Meital Segev-Bar, Gady Konvalina
  • Patent number: 10697968
    Abstract: Methods for measuring a panel of biomarkers in a subject suspected of having breast cancer are provided. The methods include obtaining a biological sample from the subject and determining a measurement for a panel of biomarkers in the biological sample, the panel comprising at least 5 biomarkers selected from the group comprising LPC(18:3), LPC(20:2), LPC(20:1), LPC(20:0), PC(32:1), PC(34:4), PC(38:3), PC(40:5), PC(40:3), PC(44:11), ePC(32:2), ePC(38:3), C19:1 CE, C19:0 CE, and C20:0 CE, wherein the measurement comprises measuring a level of each of the at least 5 biomarkers.
    Type: Grant
    Filed: October 10, 2017
    Date of Patent: June 30, 2020
    Assignee: Rush University Medical Center
    Inventor: Youping Deng
  • Patent number: 10539575
    Abstract: Biomarkers of kidney function and methods for using said biomarkers for assessing kidney function, monitoring kidney function, diagnosing acute kidney injury, and diagnosing chronic kidney disease are provided. Also provided are suites of small molecule entities as biomarkers for chronic kidney disease.
    Type: Grant
    Filed: May 13, 2014
    Date of Patent: January 21, 2020
    Assignee: METABOLON, INC.
    Inventors: Regis Perichon, Jeffery Edmond Cobb, Meredith V. Brown, Adam Kennedy
  • Patent number: 10458911
    Abstract: A method for screening an anticancer candidate is disclosed. The method includes immobilizing gold nanoparticles onto a substrate; binding a protein involved in carcinogenesis and metastasis to the immobilized gold nanoparticles, recording a spectrum of the protein conjugate, and analyzing the spectrum to obtain reference data; adding a candidate inhibiting the activity of the protein to the protein conjugate, recording a spectrum of the mixture, and analyzing the spectrum to obtain comparative data; and comparing the reference data with the comparative data to determine whether the candidate inhibits the activity of the protein. The method enables screening of a candidate inhibiting the activity of a protein involved in carcinogenesis and metastasis in an accurate and convenient manner.
    Type: Grant
    Filed: April 28, 2016
    Date of Patent: October 29, 2019
    Inventors: Sang Jun Sim, So Jin Song
  • Patent number: 10444147
    Abstract: Example implementations relate to an optical biofilm probe. For example, in an implementation, the optical biofilm probe may include a light source to project light towards a substrate disposed within a bypass and a detector to detect light from the substrate. Properties of light detected by the detector may be affected by biofilm formation on the substrate. The bypass may be connected in parallel to a conduit of a fluid system.
    Type: Grant
    Filed: July 15, 2016
    Date of Patent: October 15, 2019
    Inventors: Tahir Cader, John Franz, William K Norton
  • Patent number: 10438785
    Abstract: A method and apparatus for entering the structures of assumed sugar chains, assumed ionic numbers, m/z of a common product ion, etc. for calculating, by a precursor m/z calculator the m/z of precursor ions originating from each sugar chain. A method file creator prepares a method file including MRM transitions. A multivalent ion information file creator creates a file which associates a unique ID of each sugar-chain structure with m/z of precursor ions and m/z of a product ion. Then, a chromatogram creator creates a mass chromatogram for each MRM transition. A peak area totalizer adds up peak areas on mass chromatograms obtained for a plurality of MRM transitions corresponding to the same sugar chain. Based on the calculated total values, a quantitative value calculator calculates an abundance ratio of each sugar chain as a quantitative value. A quantitative output information creator displays the quantitative values on a display unit.
    Type: Grant
    Filed: August 31, 2015
    Date of Patent: October 8, 2019
    Inventors: Shuuichi Nakaya, Shinji Funatsu
  • Patent number: 10393762
    Abstract: The present invention relates to the field of diagnostic methods. Specifically, the present invention contemplates a method for diagnosing heart failure in a subject, a method for identifying whether a subject is in need for a therapy of heart failure or a method for determining whether a heart failure therapy is successful. The invention also relates to tools for carrying out the aforementioned methods, such as diagnostic devices.
    Type: Grant
    Filed: February 1, 2016
    Date of Patent: August 27, 2019
    Assignees: METANOMICS GMBH, Ruprecht-Karls-Universitaet Heidelberg
    Inventors: Jens Fuhrmann, Regina Reszka, Juergen Kastler, Kristina Busch, Edgar Leibold, Hugo Katus, Norbert Frey, Johanna Sigl, Tanja Weis
  • Patent number: 10388403
    Abstract: The invention provides a method for determining copy number variations (CNV) of a sequence of interest in a test sample that comprises a mixture of nucleic acids that are known or are suspected to differ in the amount of one or more sequence of interest. The method comprises a statistical approach that accounts for accrued variability stemming from process-related, interchromosomal and inter-sequencing variability. The method is applicable to determining CNV of any fetal aneuploidy, and CNVs known or suspected to be associated with a variety of medical conditions. CNV that can be determined according to the method include trisomies and monosomies of any one or more of chromosomes 1-22, X and Y, other chromosomal polysomies, and deletions and/or duplications of segments of any one or more of the chromosomes, which can be detected by sequencing only once the nucleic acids of a test sample.
    Type: Grant
    Filed: July 20, 2012
    Date of Patent: August 20, 2019
    Assignee: Verinata Health, Inc.
    Inventors: Richard P. Rava, Brian K. Rhees
  • Patent number: 10376193
    Abstract: An electrode structure is provided that includes an electrode base having topography located on a surface of the electrode base structure. A biological functionalization layer is located on one or more exposed surfaces of at least the topography of the electrode. A sacrificial layer is located on the biological functionalization layer and is present at least in the physical space located between the individual features of the topography of the electrode.
    Type: Grant
    Filed: July 25, 2016
    Date of Patent: August 13, 2019
    Assignee: International Business Machines Corporation
    Inventor: Emily R. Kinser
  • Patent number: 10309927
    Abstract: Some embodiments disclosed herein provide methods of reducing reactive molecular species in a reaction carried out in a digital fluidics device having one or more electrodes by using a reaction mixture comprising an electron acceptor additive. In some embodiments, the presence of the electron acceptor additive reduces the presence of reactive molecular species in the reaction mixture. In some embodiments, the presence of the electron acceptor additive reduces the formation of gas bubbles in the digital fluidic device. Therefore, in preferred embodiments, the electron acceptor additive does not form a gas when reduced.
    Type: Grant
    Filed: April 7, 2016
    Date of Patent: June 4, 2019
    Assignee: ILLUMINA, INC.
    Inventors: Rigo Pantoja, Rachel M. Schowalter, Jimmy Perrott, Cyril Delattre, Allen E. Eckhardt
  • Patent number: 10191063
    Abstract: The present invention provides biomarkers, methods and kits for diagnosing a Brucellosis, Q-Fever, and/or Lyme Disease, methods and kits for monitoring the effectiveness of treatment for Brucellosis, Q-Fever, or Lyme Disease, as well as methods for identifying a compound that can treat Brucellosis, Q-Fever, and/or Lyme Disease reduce or inhibit the development of complications associated with the disease in a subject, and methods to treat a Brucellosis, Q-Fever, and/or Lyme Disease.
    Type: Grant
    Filed: September 4, 2015
    Date of Patent: January 29, 2019
    Assignee: Caprion Proteomics Inc.
    Inventors: Eustache Paramithiotis, Pascal Croteau
  • Patent number: 10161007
    Abstract: Methods for the detection of components from biological samples are provided. In certain aspects, the methods may be used to detect and/or quantify specific components in a biological sample, such as tumor cells (e.g., circulating tumor cells). Systems and devices for practicing the subject methods are also provided.
    Type: Grant
    Filed: August 12, 2013
    Date of Patent: December 25, 2018
    Assignee: The Regents of the University of California
    Inventors: Adam R. Abate, Dennis Jay Eastbum, Adam R. Sciambi
  • Patent number: 10150964
    Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
    Type: Grant
    Filed: August 31, 2017
    Date of Patent: December 11, 2018
    Assignee: 10X GENOMICS, INC.
    Inventors: Benjamin Hindson, Serge Saxonov, Kevin Ness, Paul Hardenbol, Christopher Hindson, Donald Masquelier, Mirna Jarosz, Michael Schnall-Levin
  • Patent number: 10150963
    Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
    Type: Grant
    Filed: March 27, 2017
    Date of Patent: December 11, 2018
    Assignee: 10X GENOMICS, INC.
    Inventors: Benjamin Hindson, Serge Saxonov, Kevin Ness, Paul Hardenbol, Christopher Hindson, Donald Masquelier, Mirna Jarosz, Michael Schnall-Levin
  • Patent number: 10147180
    Abstract: An optical system is provided for clinical diagnostics that include methods and apparatus for rapidly detecting and characterizing rare circulating cells such as, but not limited to, circulating tumor cells/circulating stems cells (CTC/CSCs) in a biological sample. The sample is processed for analyses, loaded onto a “capture zone” in the optical system where, and subjected to a two stage optical process for very rapid detection and detailed characterization of detected cells. The detected rare cells are characterized with regards to biomarker profiles using fluorescent tags or chromophores for detection and optical imaging. Isolation of the captured rare cells is anticipated for down-stream assessments including, but not limited to, DNA, RNA, proteomic analyses and culture.
    Type: Grant
    Filed: December 18, 2014
    Date of Patent: December 4, 2018
    Assignee: AXON DX, LLC
    Inventors: Philip Couch, Celeste E. Smith, Jeffrey A. Smith, Daniel Carter
  • Patent number: 10073089
    Abstract: The présent invention relates to a method for manufacturing an analysis device including torpédo membrane fragments immobilized at the surface thereof, to the resulting analysis device, and to the use of said device for detecting, purifying, and characterizing molécules acting on nicotinic acetylcholine receptors. The présent invention is useful in the field of monitoring seafood, monitoring neurotoxic phytoplankton for the shellfish industry, monitoring the quality of bathing waters along tourist beaches, the field of monitoring fresh water reserves, the field of médical research, the field of the biological analysis and characterization of molécules, e.g., non-radioactive assays of the movement of the ligand-receptor bond on an ELISA-type microplate, thereby enabling compétitive agonists and antagonists of targets to be detected, e.g., of highly sensitive receptors.
    Type: Grant
    Filed: January 24, 2012
    Date of Patent: September 11, 2018
    Assignee: ABRAXIS LLC
    Inventors: Romulo Araoz, Hoang-Oanh Nghiem, Jordi Molgo, Luis Botana, Natalia Vilarino
  • Patent number: 10041121
    Abstract: A method and kit for detecting a genetic variant associated with a disease or disorder, including incompatibility with a pharmaceutical. The method and kit using a first nano-particle coupled to at least one morpholino nucleic acid probe comprising a target complimentary region base sequence that is a perfect match to a genetic variant sequence.
    Type: Grant
    Filed: September 17, 2014
    Date of Patent: August 7, 2018
    Assignee: Agency for Science, Technology and Research
    Inventors: Yanbing Zu, Jackie Y. Ying
  • Patent number: 10001470
    Abstract: The present invention provides a method, system and composition for screening drug candidates for cardiotoxicity and for novel drugs that effect cardiomyocyte contractility and function. The invention provides an efficient and reliable screening assay to detect the effect of new and potential drug candidates on cardiomyocyte calcium flux, membrane depolarization, and/or the propagation of action potentials.
    Type: Grant
    Filed: December 3, 2010
    Date of Patent: June 19, 2018
    Assignee: Sanford-Burnham Medical Research Institute
    Inventors: Mark Mercola, Fabio Cerignoli, Jeffrey Price
  • Patent number: 10000802
    Abstract: Disclosed is a method for determining the sequence of nucleotide bases in a polynucleotide analyte characterised by the steps of: a. generating a stream of droplets at least some of which contain a single nucleotide and wherein the order of single nucleotides in the droplet stream corresponds to the sequence of nucleotides in the analyte; b. introducing into each droplet a plurality of biological probe types each type (i) comprising a different detectable element in an undetectable state and (ii) being adapted to capture a different complimentary single nucleotide from which the analyte is constituted; c. causing the single nucleotide contained in the droplet to bind to its complimentary probe to create a used probe; and d. causing the detectable element to be released from the used probe in a detectable state.
    Type: Grant
    Filed: April 9, 2015
    Date of Patent: June 19, 2018
    Inventors: Cameron Alexander Frayling, Barnaby Balmforth, Bruno Flavio Nogueira de Sousa Soares, Thomas Henry Isaac, Boris Breiner, Alessandra Natale, Michele Amasio
  • Patent number: 9914967
    Abstract: A method of sequencing nucleic acids by probe hybridization and/or ligation is provided using DNA origami as a barcode for a nucleic acid probe.
    Type: Grant
    Filed: June 5, 2013
    Date of Patent: March 13, 2018
    Assignee: President and Fellows of Harvard College
    Inventors: George M. Church, Richard C. Terry, Frederic Vigneault
  • Patent number: 9905405
    Abstract: A method of generating an inclusion list for targeted mass spectrometric analysis is disclosed. Experimentally-acquired data for a plurality of isobarically-labeled peptides of interest is received at a computer. The data includes, for each of the isobarically-labeled peptides, a mass-to-charge (m/z) ratio, a charge state, and a chromatographic retention time. Predicted properties for a corresponding unlabeled peptide are determined via the computer. The predicted properties include a predicted m/z, a predicted charge state, and a predicted chromatographic retention time. The predicted properties for each corresponding unlabeled peptide are stored to the inclusion list. The predicted chromatographic retention time for the corresponding unlabeled peptide is determined based on hydrophobicity indices of the isobarically-labeled peptide and the corresponding unlabeled peptide, and chromatographic conditions for the targeted mass spectrometric analysis.
    Type: Grant
    Filed: February 13, 2017
    Date of Patent: February 27, 2018
    Assignee: Thermo Finnigan LLC
    Inventors: Roza I. Viner, Michael A. Blank
  • Patent number: 9736555
    Abstract: A method for cross-talk correction of intensities measured on mutually separate detection wavelength bands is presented. Each detection wavelength band relates to one of analyte-specific probe-populations contained by a sample to be analyzed. Each probe-population is capable of emitting a first signal component and a second signal component whose spectra have maxima at different wavelengths and at least the first signal component is dependent on presence of analyte detectable with that probe-population. Cross-talk corrected intensities are computed on the basis of a) the intensities measured on the detection wavelength bands, b) a value indicative of intensity occurring on an auxiliary wavelength band outside the detection wavelength bands and at least partially caused by the second signal components, and c) pre-determined cross-talk parameters.
    Type: Grant
    Filed: April 8, 2014
    Date of Patent: August 15, 2017
    Assignee: WALLAC OY
    Inventors: Ville Petteri Laitala, Raimo Harju
  • Patent number: 9707555
    Abstract: Methods and devices are provided for pretreatment of a sample containing microbial cells. In some embodiments, the pretreatment of the sample is performed via the initial selective lysis, within a sample pretreatment vessel, of non-microbial cells (such as blood cells) and the subsequent centrifugal separation of the sample to remove the resulting debris and concentrate the microbial cells. An immiscible and dense cushioning liquid may be included for collecting the microbial cells adjacent to the liquid interface formed by the cushioning liquid upon centrifugation of the pretreatment vessel. After removal of a substantial quantity of the supernatant, resuspension of the collected microbial cells, and re-establishment of the cushioning liquid interface, at least a portion of the remaining suspension may be removed without substantially removing the cushioning liquid. One or more intermediate wash cycles may be performed prior to extraction of the remaining suspension, which provides a “pretreated” sample.
    Type: Grant
    Filed: November 26, 2013
    Date of Patent: July 18, 2017
    Assignee: Qvella Corporation
    Inventors: Samad Talebpour, Aye Aye Khine, Robert Maaskant, Tino Alavie
  • Patent number: 9663824
    Abstract: Compositions and methods for identifying and treating patients having altered capacity for LC-PUFA production are disclosed.
    Type: Grant
    Filed: October 10, 2014
    Date of Patent: May 30, 2017
    Assignee: Wake Forest University Health Sciences
    Inventors: Floyd H. Chilton, Timothy D. Howard
  • Patent number: 9638695
    Abstract: A non-invasive method of detecting or screening for lung cancer in a subject specimen is provided. The method includes detecting elevated levels of one or more carbonyl-containing volatile organic compounds (VOCs) that are biomarkers for lung cancer in exhaled breath from the subject specimen. The method may further include obtaining exhaled breath from the subject specimen; forming adducts of the carbonyl-containing VOCs with a reactive chemical compound; quantifying the adducts of the carbonyl-containing VOCs to establish a subject value for each of the adducts; and comparing each subject value to a threshold healthy specimen value for each of the adducts of the carbonyl-containing VOCs. One or more subject values at quantities greater than threshold healthy specimen values are also useful for screening for lung cancer in the subject specimen.
    Type: Grant
    Filed: August 28, 2014
    Date of Patent: May 2, 2017
    Assignee: University of Louisville Research Foundation, Inc.
    Inventors: Xiaoan Fu, Michael Nantz, Michael Bousamra
  • Patent number: 9606126
    Abstract: The invention provides methods to assess protein stability and to obtain sizing information. In one aspect, the screen comprises a 94 detergent panel and a series of MWCO filtered microplates. A protein of interest is bound to an affinity matrix and aliquoted into a 96-well microplate. Wells containing the immobilized protein are washed in the new detergent and then eluted in the new detergent into a collection plate. Protein not stable in the new detergent is precipitated on the resin and not present in the elutions. Half of the elution is passed through a high (i.e., 300 kDa) MWCO microplate and the other half through a low (i.e., 100 kDa) MWCO microplate. Elutions from the microplates are spotted on a nitrocellulose membrane, visualized by Western analysis (or by some other method), and quantified. The high MWCO provides stability readout and the ratio of low/high kDa provides sizing information.
    Type: Grant
    Filed: June 29, 2010
    Date of Patent: March 28, 2017
    Assignee: University of Virgina Patent Foundation
    Inventors: Michael C. Wiener, James M. Vergis
  • Patent number: 9597687
    Abstract: The invention relates to a method for analysis of the AT/GC ratio of DNA by stretching the DNA in nanochannels and performing melting mapping of the AT/GC ratio along the DNA molecule.
    Type: Grant
    Filed: October 9, 2009
    Date of Patent: March 21, 2017
    Inventors: Jonas Tegenfeldt, Walter Reisner, Henrik Flyvbjerg
  • Patent number: 9526745
    Abstract: Embodiments of the present invention relate to methods for preparing high optical density solutions of nanoparticle, such as nanoplates, silver nanoplates or silver platelet nanoparticles, and to the solutions and substrates prepared by the methods. The process can include the addition of stabilizing agents (e.g., chemical or biological agents bound or otherwise linked to the nanoparticle surface) that stabilize the nanoparticle before, during, and/or after concentration, thereby allowing for the production of a stable, high optical density solution of silver nanoplates. The process can also include increasing the concentration of silver nanoplates within the solution, and thus increasing the solution optical density.
    Type: Grant
    Filed: November 20, 2015
    Date of Patent: December 27, 2016
    Assignees: nanoComposix, Inc., Sienna Biopharmaceuticals, Inc.
    Inventors: Steven J. Oldenburg, Martin Miranda, David S. Sebba, Todd J. Harris
  • Patent number: 9523116
    Abstract: Digital assay system, including methods and apparatus, for calculating a level of one or more targets. In an exemplary method, data for amplification of a plurality of targets including a first target may be collected from partitions. A level of the first target may be calculated from only a subset of the data that excludes partitions according to target content.
    Type: Grant
    Filed: February 3, 2014
    Date of Patent: December 20, 2016
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Svilen S. Tzonev, Claudia Litterst
  • Patent number: 9448220
    Abstract: A system and method for analyzing contaminants such as hydrocarbons in soil and ground water utilizes a reaction device comprising a catalyst encapsulated in a permeable material and processes that device in contact with a contaminant in an analytical device in order to generate a spectrogram indicative of the contaminants in the soil and ground water.
    Type: Grant
    Filed: January 18, 2013
    Date of Patent: September 20, 2016
    Inventor: John David Hanby
  • Patent number: 9441122
    Abstract: Pigment based inks are provided. The inks include a non-polar carrier fluid; and a surface-functionalized pigment particle including a nitrogen-inked moiety to the surface of the pigment particle through a nitrogen link at one end of the nitrogen-linked moiety and a segment copolymer having at least two blocks attached at another end, the pigment particle suspended in the non-polar carrier fluid. A combination of an electronic display and an electronic ink employing the pigment and a process for making the pigment-based inks are also provided.
    Type: Grant
    Filed: January 26, 2012
    Date of Patent: September 13, 2016
    Assignee: Hewlett-Packard Development Company, L.P.
    Inventors: Zhang-Lin Zhou, Qin Liu
  • Patent number: 9067167
    Abstract: The present invention refers to a modulator, to be used for comprehensive multidimensional chromatography separations, to entrap and release sample solute fractions (entrapped in a capillary loop of fixed or variable volume), deriving from a capillary column with an internal diameter ranging from 0.01 mm to 0.53 mm, onto another capillary column with an internal diameter ranging from 0.01 mm to 0.53 mm. The micro-device has been integrated in a gas chromatographic system, composed of two ovens for the independent temperature control of the two columns; the micro-device is characterized, internally, by a system of channels that enable the controlled splitting of gas flow, entering the second capillary, to generate an optimum gas linear velocity and to release the pressure in excess, with the objective of attaining the maximum separation efficiency in the second column.
    Type: Grant
    Filed: May 24, 2011
    Date of Patent: June 30, 2015
    Inventor: Luigi Mondello
  • Patent number: 9063158
    Abstract: An HPLC module utilizes a combination of compound-dedicated hardware providing line clearance between differing radiosynthesis and includes multiple compound-dedicated HPLC inject valves, each inject valve directing a fluid to a serially-connected HPLC column and UV flowcell so as to prevent cross-contamination between differing radiopharmaceutical syntheses. The module provides a disposable fluid path from each UV flowcell allowing for radioactive detection, fraction collection, formulation and final product dispensing. In this manner, a level of GMP compliance is achieved that is suitable for meeting the requirements of an MHRA approved site-license.
    Type: Grant
    Filed: October 8, 2010
    Date of Patent: June 23, 2015
    Assignee: GE Healthcare Limited
    Inventors: Colin Steel, Farah Shah, Sajinder Luthra
  • Publication number: 20150148257
    Abstract: Provided is a method for detecting analyte in a sample, which method comprises: (a) contacting the sample with a peptide nucleic acid (PNA) probe; (b) performing an electrochemical impedance spectrometry (EIS) measurement on the sample; (c) determining the presence, absence, quantity and/or identity of the analyte from the EIS measurement; wherein the analyte comprises nucleic acid; and wherein the quantity of analyte in the sample when the sample is taken is substantially the same as the quantity of analyte in the sample when the sample is subjected to the EIS measurement.
    Type: Application
    Filed: November 21, 2012
    Publication date: May 28, 2015
    Inventors: Holger Schulze, Damion Corrigan, Till Bachmann
  • Publication number: 20150148260
    Abstract: Quantitation of analytes, including but not limited to peptides, polypeptides, and proteins, in mass spectrometry using a labeled peptide coupled to a reporter, and a universal reporter.
    Type: Application
    Filed: December 22, 2014
    Publication date: May 28, 2015
    Inventors: Joel Louette, John Charles Rogers, Scott M. Peterman, Bruno Domon, Elodie Duriez
  • Publication number: 20150148261
    Abstract: Systems and methods for detecting and/or identifying target cells (e.g., bacteria) using engineered transduction particles are described herein. In some embodiments, a method includes mixing a quantity of transduction particles within a sample. The transduction particles are associated with a target cell. The transduction particles are non-replicative, and are engineered to include a nucleic acid molecule formulated to cause the target cell to produce a series of reporter molecules. The sample and the transduction particles are maintained to express the series of the reporter molecules when target cell is present in the sample. A signal associated with a quantity of the reporter molecules is received. In some embodiments, a magnitude of the signal is independent from a quantity of the transduction particle above a predetermined quantity.
    Type: Application
    Filed: February 2, 2015
    Publication date: May 28, 2015
    Inventors: Werner FREI, Diego Ariel REY, Shaunak ROY, Ryan C. GRISWOLD, Kenneth G. OLSON, Bruce J. RICHARDSON, Rick V. STELLMACHER
  • Publication number: 20150148259
    Abstract: Novel phenyl-glyoxal based anti-citrulline probes and methods of synthesis are provided. Methods of use, such as, the development of methods for monitoring substrate citrullination over time; for identifying citrullinated proteins from cells are described.
    Type: Application
    Filed: June 4, 2013
    Publication date: May 28, 2015
    Inventors: Paul R. Thompson, Kevin L. Bicker, Venkataraman Subramanian
  • Publication number: 20150148258
    Abstract: The present invention describes a method and devices for the simultaneous detection, recovery identification and counting of a plurality of microorganisms consisting in providing mixtures of nutrients specially selected from those that curtail the lag phase of growth in bacteria and moulds and which, together with fluorescent enzymatic, chromogenic or bioluminescent markers and other nutrient components or growth inhibitors, are embedded in three-dimensional structures or natural or artificial clays or ceramics with cavities of different dimensions and forms and specific surface areas of between 2×103 and 6×108 m2/m3.
    Type: Application
    Filed: March 27, 2013
    Publication date: May 28, 2015
    Inventors: Claudio Rodriguez Martinez, Jesus Eduardo Gonzalez Ruiz, Tamara Lobaina Rodriguez, Raisa Zhurbenko, Ana Iris Brito Gonzales, Monica Lopez Hernandez, Javier Fernandez Aragon, Ivonne Alfonso Valdes, Adelaida Ortea Suris
  • Publication number: 20150141291
    Abstract: A self-contained, fully automated, biological assay-performing apparatus includes a housing; a dispensing platform including a controllably-movable reagent dispensing system, disposed in the housing; a reagent supply component disposed in the housing; a pneumatic manifold removably disposed in the housing in a space shared by the dispensing platform, removably coupled to a fluidic transport layer and a plurality of reservoirs, wherein the fluidic transport layer, the reservoirs, and a test sample to be introduced therein are disposed in the housing in the space separate from the dispensing platform; a pneumatic supply system removably coupled to the pneumatic manifold in the housing in a space separate from the dispensing platform; and a control system coupled to at least one of the dispensing platform and the pneumatic supply system, disposed in the housing.
    Type: Application
    Filed: January 30, 2015
    Publication date: May 21, 2015
    Applicant: RHEONIX, INC.
    Inventors: Peng Zhou, Lincoln C. Young, Benjamin Thomas, Zongyuan Chen, Todd Roswech, Gwendolyn Spizz, Rubina Yasmin, Greg Mouchka
  • Publication number: 20150140039
    Abstract: The present invention concerns embodiments for determining whether an individual in need of immunotherapy will be responsive to the immunotherapy. Determination of one or more SNPs in particular genes is predictive of responsiveness to immunotherapy, particularly in individuals that have melanoma, for example. In certain embodiments, the SNPs are in ITGB2, SP1 1O, ILIB, IL23R, SLC11A1, IL12B, CCR5, TNF, ILIO, CXCL12, BTNL2, ANKRD20A4, CD 14, P2X7, IL8, TLR2, and/or CD209.
    Type: Application
    Filed: May 16, 2013
    Publication date: May 21, 2015
    Inventors: Dave S.B. Hoon, Donald L. Morton, Connie G. Chiu, Kelly Chong
  • Publication number: 20150141290
    Abstract: The present invention relates to a method for quantifying the relative content of a protein in a sample. The present invention also relates to a method for comprising the relative content of a protein in at least two samples.
    Type: Application
    Filed: December 31, 2011
    Publication date: May 21, 2015
    Inventors: Haiyi Zhao, Dahai Jiang, Bo Wen, Jian Wang, Jun Wang, Huanming Yang
  • Publication number: 20150133331
    Abstract: Methods for the detection and screening of hepatocellular carcinoma (HCC) patients and for the monitoring of HCC treatment using a panel or panels of small molecule metabolite biomarkers are disclosed. In other aspects, methods for detection and screening for the progression of high-risk conditions, such as HCV infections, to HCC and to monitoring treatment using a panel or panels of small molecule metabolite biomarkers are disclosed. The biomarkers are sensitive and specific for the detection of HCC, and can also be used to classify HCV infections that are regarded as precursors of HCC.
    Type: Application
    Filed: May 21, 2013
    Publication date: May 14, 2015
    Applicant: Purdue Research Foundation
    Inventors: Michael Daniel Raftery, Jeremiah Bowers, Siwei Wei, Hamid Reza Baniasadi
  • Publication number: 20150133312
    Abstract: The present disclosure relates to compositions and methods for detecting rare nucleic acid molecule mutations in a plurality of nucleic acid molecules. Also disclosed are methods for determining the size of a nucleic acid molecule using droplet digital PCR.
    Type: Application
    Filed: May 29, 2013
    Publication date: May 14, 2015
    Inventors: Jason H. Bielas, Sean D. Taylor, Mathew T. Laurie
  • Publication number: 20150133343
    Abstract: A method for the diagnosis of prostate cancer, comprising the step of determining the presence or absence of at least three polypeptide markers in a sample, wherein the polypeptide marker is selected from markers 1 to 44 and 52 to 78 (frequency markers), or determining the amplitude of at least one polypeptide marker selected from markers 45 to 51 and 79 to 115 (amplitude markers), wherein said sample is a urine sample or seminal fluid sample.
    Type: Application
    Filed: December 29, 2014
    Publication date: May 14, 2015
    Inventor: Harald Mischak
  • Publication number: 20150133342
    Abstract: The present invention relates to mass spectrometry methods employing multiple reaction monitoring (MRM) in the field of cancer therapeutics, specifically prostate cancer and melanoma.
    Type: Application
    Filed: May 3, 2013
    Publication date: May 14, 2015
    Inventors: Sun W. Tam, Ying Xu, Patrick J. Muraca
  • Publication number: 20150126405
    Abstract: A method for recognizing rejection after a kidney transplantation (NTx), comprising the step of determining the presence or absence of at least one polypeptide marker in a sample, wherein said polypeptide marker is selected from markers 1 to 242 (frequency markers), or determining the amplitude of at least one polypeptide marker selected from markers 243 to 767 (amplitude markers), which are characterized by the values for the molecular masses and migration times (CE time).
    Type: Application
    Filed: November 26, 2014
    Publication date: May 7, 2015
    Inventors: Harald Mischak, Stefan Wittke
  • Publication number: 20150126404
    Abstract: A triple sensor structured for simultaneous measurement of glucose, oxygen, and pH. The sensor components are in thin film states such as sensing films or membranes, with a glucose probe associated with emission of radiation in the blue part of the spectrum, an oxygen probe associated with radiation in red portion of the spectrum, and a pH probe—with a green portion of the spectrum. The optical probes are chemically grafted or immobilized in a suitable polymer matrix, alleviating the leaching of the probes from the matrix, improving the thin film sensing stability, and enabling the repeatable use of the same sensing films.
    Type: Application
    Filed: November 5, 2014
    Publication date: May 7, 2015
    Inventors: Yanqing Tian, Liqiang Zhang, Fengyu Su, Deirdre Meldrum, Sean Buizer, Clifford L. Anderson, Laimonas Kelbauskas, Kristen Lee