By Measuring A Physical Property (e.g., Mass, Etc.) Patents (Class 506/12)
  • Patent number: 9736555
    Abstract: A method for cross-talk correction of intensities measured on mutually separate detection wavelength bands is presented. Each detection wavelength band relates to one of analyte-specific probe-populations contained by a sample to be analyzed. Each probe-population is capable of emitting a first signal component and a second signal component whose spectra have maxima at different wavelengths and at least the first signal component is dependent on presence of analyte detectable with that probe-population. Cross-talk corrected intensities are computed on the basis of a) the intensities measured on the detection wavelength bands, b) a value indicative of intensity occurring on an auxiliary wavelength band outside the detection wavelength bands and at least partially caused by the second signal components, and c) pre-determined cross-talk parameters.
    Type: Grant
    Filed: April 8, 2014
    Date of Patent: August 15, 2017
    Assignee: WALLAC OY
    Inventors: Ville Petteri Laitala, Raimo Harju
  • Patent number: 9707555
    Abstract: Methods and devices are provided for pretreatment of a sample containing microbial cells. In some embodiments, the pretreatment of the sample is performed via the initial selective lysis, within a sample pretreatment vessel, of non-microbial cells (such as blood cells) and the subsequent centrifugal separation of the sample to remove the resulting debris and concentrate the microbial cells. An immiscible and dense cushioning liquid may be included for collecting the microbial cells adjacent to the liquid interface formed by the cushioning liquid upon centrifugation of the pretreatment vessel. After removal of a substantial quantity of the supernatant, resuspension of the collected microbial cells, and re-establishment of the cushioning liquid interface, at least a portion of the remaining suspension may be removed without substantially removing the cushioning liquid. One or more intermediate wash cycles may be performed prior to extraction of the remaining suspension, which provides a “pretreated” sample.
    Type: Grant
    Filed: November 26, 2013
    Date of Patent: July 18, 2017
    Assignee: Qvella Corporation
    Inventors: Samad Talebpour, Aye Aye Khine, Robert Maaskant, Tino Alavie
  • Patent number: 9663824
    Abstract: Compositions and methods for identifying and treating patients having altered capacity for LC-PUFA production are disclosed.
    Type: Grant
    Filed: October 10, 2014
    Date of Patent: May 30, 2017
    Assignee: Wake Forest University Health Sciences
    Inventors: Floyd H. Chilton, Timothy D. Howard
  • Patent number: 9638695
    Abstract: A non-invasive method of detecting or screening for lung cancer in a subject specimen is provided. The method includes detecting elevated levels of one or more carbonyl-containing volatile organic compounds (VOCs) that are biomarkers for lung cancer in exhaled breath from the subject specimen. The method may further include obtaining exhaled breath from the subject specimen; forming adducts of the carbonyl-containing VOCs with a reactive chemical compound; quantifying the adducts of the carbonyl-containing VOCs to establish a subject value for each of the adducts; and comparing each subject value to a threshold healthy specimen value for each of the adducts of the carbonyl-containing VOCs. One or more subject values at quantities greater than threshold healthy specimen values are also useful for screening for lung cancer in the subject specimen.
    Type: Grant
    Filed: August 28, 2014
    Date of Patent: May 2, 2017
    Assignee: University of Louisville Research Foundation, Inc.
    Inventors: Xiaoan Fu, Michael Nantz, Michael Bousamra
  • Patent number: 9606126
    Abstract: The invention provides methods to assess protein stability and to obtain sizing information. In one aspect, the screen comprises a 94 detergent panel and a series of MWCO filtered microplates. A protein of interest is bound to an affinity matrix and aliquoted into a 96-well microplate. Wells containing the immobilized protein are washed in the new detergent and then eluted in the new detergent into a collection plate. Protein not stable in the new detergent is precipitated on the resin and not present in the elutions. Half of the elution is passed through a high (i.e., 300 kDa) MWCO microplate and the other half through a low (i.e., 100 kDa) MWCO microplate. Elutions from the microplates are spotted on a nitrocellulose membrane, visualized by Western analysis (or by some other method), and quantified. The high MWCO provides stability readout and the ratio of low/high kDa provides sizing information.
    Type: Grant
    Filed: June 29, 2010
    Date of Patent: March 28, 2017
    Assignee: University of Virgina Patent Foundation
    Inventors: Michael C. Wiener, James M. Vergis
  • Patent number: 9597687
    Abstract: The invention relates to a method for analysis of the AT/GC ratio of DNA by stretching the DNA in nanochannels and performing melting mapping of the AT/GC ratio along the DNA molecule.
    Type: Grant
    Filed: October 9, 2009
    Date of Patent: March 21, 2017
    Inventors: Jonas Tegenfeldt, Walter Reisner, Henrik Flyvbjerg
  • Patent number: 9526745
    Abstract: Embodiments of the present invention relate to methods for preparing high optical density solutions of nanoparticle, such as nanoplates, silver nanoplates or silver platelet nanoparticles, and to the solutions and substrates prepared by the methods. The process can include the addition of stabilizing agents (e.g., chemical or biological agents bound or otherwise linked to the nanoparticle surface) that stabilize the nanoparticle before, during, and/or after concentration, thereby allowing for the production of a stable, high optical density solution of silver nanoplates. The process can also include increasing the concentration of silver nanoplates within the solution, and thus increasing the solution optical density.
    Type: Grant
    Filed: November 20, 2015
    Date of Patent: December 27, 2016
    Assignees: nanoComposix, Inc., Sienna Biopharmaceuticals, Inc.
    Inventors: Steven J. Oldenburg, Martin Miranda, David S. Sebba, Todd J. Harris
  • Patent number: 9523116
    Abstract: Digital assay system, including methods and apparatus, for calculating a level of one or more targets. In an exemplary method, data for amplification of a plurality of targets including a first target may be collected from partitions. A level of the first target may be calculated from only a subset of the data that excludes partitions according to target content.
    Type: Grant
    Filed: February 3, 2014
    Date of Patent: December 20, 2016
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Svilen S. Tzonev, Claudia Litterst
  • Patent number: 9448220
    Abstract: A system and method for analyzing contaminants such as hydrocarbons in soil and ground water utilizes a reaction device comprising a catalyst encapsulated in a permeable material and processes that device in contact with a contaminant in an analytical device in order to generate a spectrogram indicative of the contaminants in the soil and ground water.
    Type: Grant
    Filed: January 18, 2013
    Date of Patent: September 20, 2016
    Inventor: John David Hanby
  • Patent number: 9441122
    Abstract: Pigment based inks are provided. The inks include a non-polar carrier fluid; and a surface-functionalized pigment particle including a nitrogen-inked moiety to the surface of the pigment particle through a nitrogen link at one end of the nitrogen-linked moiety and a segment copolymer having at least two blocks attached at another end, the pigment particle suspended in the non-polar carrier fluid. A combination of an electronic display and an electronic ink employing the pigment and a process for making the pigment-based inks are also provided.
    Type: Grant
    Filed: January 26, 2012
    Date of Patent: September 13, 2016
    Assignee: Hewlett-Packard Development Company, L.P.
    Inventors: Zhang-Lin Zhou, Qin Liu
  • Patent number: 9067167
    Abstract: The present invention refers to a modulator, to be used for comprehensive multidimensional chromatography separations, to entrap and release sample solute fractions (entrapped in a capillary loop of fixed or variable volume), deriving from a capillary column with an internal diameter ranging from 0.01 mm to 0.53 mm, onto another capillary column with an internal diameter ranging from 0.01 mm to 0.53 mm. The micro-device has been integrated in a gas chromatographic system, composed of two ovens for the independent temperature control of the two columns; the micro-device is characterized, internally, by a system of channels that enable the controlled splitting of gas flow, entering the second capillary, to generate an optimum gas linear velocity and to release the pressure in excess, with the objective of attaining the maximum separation efficiency in the second column.
    Type: Grant
    Filed: May 24, 2011
    Date of Patent: June 30, 2015
    Inventor: Luigi Mondello
  • Patent number: 9063158
    Abstract: An HPLC module utilizes a combination of compound-dedicated hardware providing line clearance between differing radiosynthesis and includes multiple compound-dedicated HPLC inject valves, each inject valve directing a fluid to a serially-connected HPLC column and UV flowcell so as to prevent cross-contamination between differing radiopharmaceutical syntheses. The module provides a disposable fluid path from each UV flowcell allowing for radioactive detection, fraction collection, formulation and final product dispensing. In this manner, a level of GMP compliance is achieved that is suitable for meeting the requirements of an MHRA approved site-license.
    Type: Grant
    Filed: October 8, 2010
    Date of Patent: June 23, 2015
    Assignee: GE Healthcare Limited
    Inventors: Colin Steel, Farah Shah, Sajinder Luthra
  • Publication number: 20150148257
    Abstract: Provided is a method for detecting analyte in a sample, which method comprises: (a) contacting the sample with a peptide nucleic acid (PNA) probe; (b) performing an electrochemical impedance spectrometry (EIS) measurement on the sample; (c) determining the presence, absence, quantity and/or identity of the analyte from the EIS measurement; wherein the analyte comprises nucleic acid; and wherein the quantity of analyte in the sample when the sample is taken is substantially the same as the quantity of analyte in the sample when the sample is subjected to the EIS measurement.
    Type: Application
    Filed: November 21, 2012
    Publication date: May 28, 2015
    Inventors: Holger Schulze, Damion Corrigan, Till Bachmann
  • Publication number: 20150148261
    Abstract: Systems and methods for detecting and/or identifying target cells (e.g., bacteria) using engineered transduction particles are described herein. In some embodiments, a method includes mixing a quantity of transduction particles within a sample. The transduction particles are associated with a target cell. The transduction particles are non-replicative, and are engineered to include a nucleic acid molecule formulated to cause the target cell to produce a series of reporter molecules. The sample and the transduction particles are maintained to express the series of the reporter molecules when target cell is present in the sample. A signal associated with a quantity of the reporter molecules is received. In some embodiments, a magnitude of the signal is independent from a quantity of the transduction particle above a predetermined quantity.
    Type: Application
    Filed: February 2, 2015
    Publication date: May 28, 2015
    Applicant: GENEWEAVE BIOSCIENCES, INC.
    Inventors: Werner FREI, Diego Ariel REY, Shaunak ROY, Ryan C. GRISWOLD, Kenneth G. OLSON, Bruce J. RICHARDSON, Rick V. STELLMACHER
  • Publication number: 20150148258
    Abstract: The present invention describes a method and devices for the simultaneous detection, recovery identification and counting of a plurality of microorganisms consisting in providing mixtures of nutrients specially selected from those that curtail the lag phase of growth in bacteria and moulds and which, together with fluorescent enzymatic, chromogenic or bioluminescent markers and other nutrient components or growth inhibitors, are embedded in three-dimensional structures or natural or artificial clays or ceramics with cavities of different dimensions and forms and specific surface areas of between 2×103 and 6×108 m2/m3.
    Type: Application
    Filed: March 27, 2013
    Publication date: May 28, 2015
    Inventors: Claudio Rodriguez Martinez, Jesus Eduardo Gonzalez Ruiz, Tamara Lobaina Rodriguez, Raisa Zhurbenko, Ana Iris Brito Gonzales, Monica Lopez Hernandez, Javier Fernandez Aragon, Ivonne Alfonso Valdes, Adelaida Ortea Suris
  • Publication number: 20150148259
    Abstract: Novel phenyl-glyoxal based anti-citrulline probes and methods of synthesis are provided. Methods of use, such as, the development of methods for monitoring substrate citrullination over time; for identifying citrullinated proteins from cells are described.
    Type: Application
    Filed: June 4, 2013
    Publication date: May 28, 2015
    Inventors: Paul R. Thompson, Kevin L. Bicker, Venkataraman Subramanian
  • Publication number: 20150148260
    Abstract: Quantitation of analytes, including but not limited to peptides, polypeptides, and proteins, in mass spectrometry using a labeled peptide coupled to a reporter, and a universal reporter.
    Type: Application
    Filed: December 22, 2014
    Publication date: May 28, 2015
    Inventors: Joel Louette, John Charles Rogers, Scott M. Peterman, Bruno Domon, Elodie Duriez
  • Publication number: 20150140039
    Abstract: The present invention concerns embodiments for determining whether an individual in need of immunotherapy will be responsive to the immunotherapy. Determination of one or more SNPs in particular genes is predictive of responsiveness to immunotherapy, particularly in individuals that have melanoma, for example. In certain embodiments, the SNPs are in ITGB2, SP1 1O, ILIB, IL23R, SLC11A1, IL12B, CCR5, TNF, ILIO, CXCL12, BTNL2, ANKRD20A4, CD 14, P2X7, IL8, TLR2, and/or CD209.
    Type: Application
    Filed: May 16, 2013
    Publication date: May 21, 2015
    Inventors: Dave S.B. Hoon, Donald L. Morton, Connie G. Chiu, Kelly Chong
  • Publication number: 20150141291
    Abstract: A self-contained, fully automated, biological assay-performing apparatus includes a housing; a dispensing platform including a controllably-movable reagent dispensing system, disposed in the housing; a reagent supply component disposed in the housing; a pneumatic manifold removably disposed in the housing in a space shared by the dispensing platform, removably coupled to a fluidic transport layer and a plurality of reservoirs, wherein the fluidic transport layer, the reservoirs, and a test sample to be introduced therein are disposed in the housing in the space separate from the dispensing platform; a pneumatic supply system removably coupled to the pneumatic manifold in the housing in a space separate from the dispensing platform; and a control system coupled to at least one of the dispensing platform and the pneumatic supply system, disposed in the housing.
    Type: Application
    Filed: January 30, 2015
    Publication date: May 21, 2015
    Applicant: RHEONIX, INC.
    Inventors: Peng Zhou, Lincoln C. Young, Benjamin Thomas, Zongyuan Chen, Todd Roswech, Gwendolyn Spizz, Rubina Yasmin, Greg Mouchka
  • Publication number: 20150141290
    Abstract: The present invention relates to a method for quantifying the relative content of a protein in a sample. The present invention also relates to a method for comprising the relative content of a protein in at least two samples.
    Type: Application
    Filed: December 31, 2011
    Publication date: May 21, 2015
    Applicant: BGI TECH SOLUTIONS CO., LTD.
    Inventors: Haiyi Zhao, Dahai Jiang, Bo Wen, Jian Wang, Jun Wang, Huanming Yang
  • Publication number: 20150133343
    Abstract: A method for the diagnosis of prostate cancer, comprising the step of determining the presence or absence of at least three polypeptide markers in a sample, wherein the polypeptide marker is selected from markers 1 to 44 and 52 to 78 (frequency markers), or determining the amplitude of at least one polypeptide marker selected from markers 45 to 51 and 79 to 115 (amplitude markers), wherein said sample is a urine sample or seminal fluid sample.
    Type: Application
    Filed: December 29, 2014
    Publication date: May 14, 2015
    Inventor: Harald Mischak
  • Publication number: 20150133312
    Abstract: The present disclosure relates to compositions and methods for detecting rare nucleic acid molecule mutations in a plurality of nucleic acid molecules. Also disclosed are methods for determining the size of a nucleic acid molecule using droplet digital PCR.
    Type: Application
    Filed: May 29, 2013
    Publication date: May 14, 2015
    Inventors: Jason H. Bielas, Sean D. Taylor, Mathew T. Laurie
  • Publication number: 20150133331
    Abstract: Methods for the detection and screening of hepatocellular carcinoma (HCC) patients and for the monitoring of HCC treatment using a panel or panels of small molecule metabolite biomarkers are disclosed. In other aspects, methods for detection and screening for the progression of high-risk conditions, such as HCV infections, to HCC and to monitoring treatment using a panel or panels of small molecule metabolite biomarkers are disclosed. The biomarkers are sensitive and specific for the detection of HCC, and can also be used to classify HCV infections that are regarded as precursors of HCC.
    Type: Application
    Filed: May 21, 2013
    Publication date: May 14, 2015
    Applicant: Purdue Research Foundation
    Inventors: Michael Daniel Raftery, Jeremiah Bowers, Siwei Wei, Hamid Reza Baniasadi
  • Publication number: 20150133342
    Abstract: The present invention relates to mass spectrometry methods employing multiple reaction monitoring (MRM) in the field of cancer therapeutics, specifically prostate cancer and melanoma.
    Type: Application
    Filed: May 3, 2013
    Publication date: May 14, 2015
    Inventors: Sun W. Tam, Ying Xu, Patrick J. Muraca
  • Publication number: 20150126403
    Abstract: A method of identifying proteins present in human serum which are differentially expressed between normal individuals and patients known to have non-small cell lung cancers and asthma, as diagnosed by a physician. Human serum specimens from each population are digested with trypsin or any other suitable endoproteinase and analyzed using a liquid chromatography electrospray ionization mass spectrometer. Mass spectral data from each population is compared to determine proteins with expression intensities which are significantly differentially expressed between the normal, asthma, and lung cancer populations. Eleven proteins are found to have expression intensities which are significantly differentially expressed between the populations. Finally, the identities of the eleven proteins are obtained by comparing the mass spectral data with known databases having libraries of mass spectral data of known proteins.
    Type: Application
    Filed: August 21, 2014
    Publication date: May 7, 2015
    Inventors: Sung H. BAEK, Robert T. STREEPER, Elzbieta IZBICKA
  • Publication number: 20150126404
    Abstract: A triple sensor structured for simultaneous measurement of glucose, oxygen, and pH. The sensor components are in thin film states such as sensing films or membranes, with a glucose probe associated with emission of radiation in the blue part of the spectrum, an oxygen probe associated with radiation in red portion of the spectrum, and a pH probe—with a green portion of the spectrum. The optical probes are chemically grafted or immobilized in a suitable polymer matrix, alleviating the leaching of the probes from the matrix, improving the thin film sensing stability, and enabling the repeatable use of the same sensing films.
    Type: Application
    Filed: November 5, 2014
    Publication date: May 7, 2015
    Inventors: Yanqing Tian, Liqiang Zhang, Fengyu Su, Deirdre Meldrum, Sean Buizer, Clifford L. Anderson, Laimonas Kelbauskas, Kristen Lee
  • Publication number: 20150126402
    Abstract: A method for quantifying metallothionein protein isomers is described herein. Such metallothionein isomer protein quantification is useful for detecting and monitoring disease. As illustrated herein, protein quantification is a more accurate measure of metallothionein induction than is mRNA quantification.
    Type: Application
    Filed: May 17, 2013
    Publication date: May 7, 2015
    Inventors: John B. Shabb, Aaron Mehus, Wallace Muhonen, Donald A. Sens, Scott Garrett
  • Publication number: 20150126405
    Abstract: A method for recognizing rejection after a kidney transplantation (NTx), comprising the step of determining the presence or absence of at least one polypeptide marker in a sample, wherein said polypeptide marker is selected from markers 1 to 242 (frequency markers), or determining the amplitude of at least one polypeptide marker selected from markers 243 to 767 (amplitude markers), which are characterized by the values for the molecular masses and migration times (CE time).
    Type: Application
    Filed: November 26, 2014
    Publication date: May 7, 2015
    Inventors: Harald Mischak, Stefan Wittke
  • Publication number: 20150119286
    Abstract: A high-throughput corrosion testing mechanism was developed for metals in a variety of environments in controlled, multiplexed microenvironments. Many parallel experiments can be conducted with microbial and environmental variables independently manipulated to identify the key determinants of corrosion progression. The synthetic assay design enables subsequent surface characterization of select samples within the array. In as little as one day, diverse corrosive environments can be compared quantitatively.
    Type: Application
    Filed: October 29, 2014
    Publication date: April 30, 2015
    Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
    Inventors: Matthew James Whitfield, Krystyn J. Van Viet
  • Publication number: 20150118155
    Abstract: The present invention provides a method of diagnosing the existence or risk of hyperthyroidism in a feline comprising measuring the level of expression of one or more biomarkers selected from the group consisting of e.g., IYD, TG, SLC5A5, NIS, TPO, TSHR, DUOX1, DUOX2 (ThOX), TGFB1, CSTD, DCN and SEPP1 and the expression products thereof, in a biological sample from the feline, wherein elevated expression of the one or more biomarkers in the sample relative to a control value for expression in a sample from a normal feline or feline population, or a baseline value from the feline, indicates the existence or risk of hyperthyroidism; a method of treating a feline so diagnosed; and compositions, reagents and kits for carrying out the specified methods.
    Type: Application
    Filed: June 14, 2012
    Publication date: April 30, 2015
    Applicant: Hill's Pet Nutrition, Inc.
    Inventors: Samer Al-Murrani, Xiangming Gao
  • Publication number: 20150119285
    Abstract: A magnetic-assisted rapid aptamer selection (MARAS) protocol for screening DNA aptamer is proposed. The MARAS protocol is able to efficiently generate aptamers with high affinity and specificity. A rotating magnetic field or alternating magnetic field was used in combination with target-bound magnetic micro-particles or nanoparticles to select DNA aptamers having desirable affinity and specificity to the target.
    Type: Application
    Filed: October 28, 2013
    Publication date: April 30, 2015
    Inventors: Chin-Yih Hong, Chi-Ching Lai
  • Patent number: 9018137
    Abstract: The document pertains to a method for the purification of a ternary mixture of dimeric antibodies of the type AA, AB, BB, characterized in that for the separation of the three components and in particular for the isolation of the multi-specific fraction AB multicolumn counter current solvent gradient purification chromatography with a stationary phase load of more than 1 mg antibody mixture per milliliter stationary phase is used. It furthermore relates to a method for the identification of in particular bispecific antibody systems, which are particularly suitable for the application of such a purification method.
    Type: Grant
    Filed: March 15, 2012
    Date of Patent: April 28, 2015
    Assignee: ChromaCon AG
    Inventors: Thomas Müller-Späth, Lars Aumann, Guido Ströhlein, Michael Bavand
  • Publication number: 20150110773
    Abstract: Methods and kits for GPP-targeting, e.g., for the treatment of oncogenic Kras-associated cancers, and methods for determining the efficacy of those methods are provided.
    Type: Application
    Filed: May 23, 2013
    Publication date: April 23, 2015
    Inventors: Alec C. Kimmelman, Jaekyoung Son, Lewis Cantley, Costas A. Lyssiotis
  • Publication number: 20150111787
    Abstract: Described herein is a digital microfluidic droplet generator (DMDG) and a microfluidic platform for processing material introduced into the DMDG. The combination is particularly suited for hydrogen/deuterium exchange and mass spectrometer (HDX-MS) processing and analysis of membrane proteins.
    Type: Application
    Filed: June 4, 2012
    Publication date: April 23, 2015
    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Kwang-Fu C. Shen, Julian P. Whitelegge, Kym Francis Faull, Libo Zhao, Christopher Miles Ryan
  • Publication number: 20150111942
    Abstract: Indole-3-propionic acid as a marker and for treatment for Huntington Disease.
    Type: Application
    Filed: October 15, 2014
    Publication date: April 23, 2015
    Inventor: Wayne R. Matson
  • Publication number: 20150105296
    Abstract: Using NMR/MS based metabonomics and targeted lipidomics approaches the inventors have explored the metabolic phenotypes of aging and longevity in a cohort compromising centenarians, elderly and young adults. The inventors have identified biomarkers for a reduced risk of developing ageing related chronic inflammatory disorders and propose a method of diagnosing a lifestyle that allows delaying and/or avoiding ageing related chronic inflammatory disorders using hydroxy-sphingomyelin SM-OH-22:1 as biomarker.
    Type: Application
    Filed: September 19, 2014
    Publication date: April 16, 2015
    Inventors: Sebastiano Collino, Ivan Montoliu Roura, Francois-Pierre Martin, Philippe Alexandre Guy, Serge Andre Dominique Rezzi
  • Publication number: 20150105291
    Abstract: It is intended to evaluate an ischemic heart disease with high accuracy by convenient operation. The method for evaluating an ischemic heart disease according to the present invention comprises the steps of: assaying complement factor H and/or complement factor D in a sample derived from the blood of a test subject; and comparing the concentration of the complement factor H and/or the concentration of the complement factor D assayed in the preceding step with a reference value(s), wherein it is determined that the seriousness of the ischemic heart disease is high when the concentration falls below the reference value.
    Type: Application
    Filed: October 15, 2014
    Publication date: April 16, 2015
    Inventors: Hiroko HANZAWA, Takeshi SAKAMOTO, Yuji KUGE
  • Publication number: 20150105293
    Abstract: The inventors have proposed a novel panel of human plasma protein biomarkers for diagnosing hepatic fibrosis and cirrhosis. Presently there is no reliable non-invasive way of assessing liver fibrosis. A 2D-PAGE based proteomics study was used to identify potential fibrosis biomarkers. Plasma from patients with hepatic cirrhosis induced by infection with the hepatitis C virus (HCV) were analysed. Several proteins associated with liver scarring and potentially also related to viral infection were identified. These proteins include 14-3-3 protein zeta/delta, adiponectin, afamin, alpha-1-antitrypsin, alpha-2-HS-glycoprotein, apolipoprotein C-M, apolipoprotein E, C4b-binding protein beta chain, intact/cleaved complement C3dg, corticosteroid-binding globulin, fibrinogen gamma chain, beta haptoglobin at pH 5.46-5.
    Type: Application
    Filed: October 23, 2014
    Publication date: April 16, 2015
    Applicant: The Chancellor, Masters and Scholars of The University of Oxford
    Inventors: Bevin Gangadharan, Nicole Titzmann, Raymond A. Dwek
  • Publication number: 20150104440
    Abstract: The current disclosure reveals a complex regulatory pattern between miR-31 and AR, indicating that miR-31 plays a key role in prostate cancer development and progression. Another aspect of the current disclosure shows that miR-31 directly targets and destabilizes AR mRNA through interaction with the AR mRNA coding sequence showing that miR-31, or a fragment thereof has the ability to act as a novel therapeutic agent in treating cancer. The current disclosure also shows that AR indirectly represses miR-31 expression by binding to the miR-31 promoter region and modulating methyltransferase activity. Another aspect of the current disclosure shows that miR-31 indirectly modulates AR activity by modulating regulators of cell cycle progression. The disclosure further provides an isolated nucleic acid that modulates the activity of the androgen receptor in a cell.
    Type: Application
    Filed: April 12, 2013
    Publication date: April 16, 2015
    Applicant: CORNELL UNIVERSITY
    Inventors: Pei-Chun Lin, Mark A. Rubin
  • Publication number: 20150105297
    Abstract: A micro-fluidic device for mapping a DNA or RNA strand labeled at a plurality of specific sites with labels suitable for generating a detection signal when interacting with a detector element, the device comprising: a micro-fluidic channel; and a plurality of detector elements for detecting the labels by acquiring the detection signals, the detector elements being positioned longitudinally along the micro-fluidic channel, each detector element having a width, successive detector elements being separated by an inter-detector gap having a width, wherein the widths of at least two of the detector elements are different and/or wherein the widths at least two of the inter-detector gaps are different.
    Type: Application
    Filed: October 13, 2014
    Publication date: April 16, 2015
    Applicants: Katholieke Universiteit Leuven, KU LEUVEN R&D, IMEC VZW
    Inventors: Tim Stakenborg, Robert Neely, Pol Van Dorpe, Johan Hofkens
  • Publication number: 20150099664
    Abstract: A method for characterizing the risk a subject will develop an autoimmune and/or alloimmune disease following tissue transplant includes obtaining a biological sample from the subject, wherein the subject has received the tissue transplant determining in the biological sample a level of at least one protein selected from Tables 1-4, comparing the measured level of the at least one protein to a control value, and characterizing a subject as at greater risk of developing an autoimmune disease and/or alloimmune disease if the level of at least one protein determined is increased or decreased compared to the control value.
    Type: Application
    Filed: December 15, 2014
    Publication date: April 9, 2015
    Inventors: Mark Chance, Kenneth Cooke, Daniela Schlatzer
  • Publication number: 20150099669
    Abstract: The presently disclosed subject matter provides methods using mass spectrometry for direct profiling of N-linked glycans from a biological sample. In addition, the embodiments of the present invention also disclose novel methods, known as targeted analyte detection (TAD), for improving the detection limit of MALDI-MS. These methods take advantage of the carrier effect of the added standard analytes, which occurs due to the generic sigmoidal shape of the calibration curve. The functionality of TAD depends on the relative enhancement of sensitivity over the increase of the standard deviation at the analysis of target analytes with spiking in exogenous concentration. At certain ranges of exogenous concentration, the increment in the sensitivity overcomes the standard deviation, resulting in an improved LOD. Theoretically, exogenous concentrations approximately at 1 LODorig would generate the optimum LOD improvement.
    Type: Application
    Filed: May 23, 2013
    Publication date: April 9, 2015
    Inventors: Hui Zhang, Xingde Li, Shadi Toghi Eshghi
  • Publication number: 20150099668
    Abstract: This document provides methods and materials for assessing metabolites in a sample from a mammal (e.g., human) for determining the nature and extent of colorectal cancer (CRC) metastasis. For example, the document relates to the diagnosis, staging and prognosis of CRC in a mammal.
    Type: Application
    Filed: May 14, 2013
    Publication date: April 9, 2015
    Applicant: UTI LIMITED PARTNERSHIP
    Inventors: Farshad Farshidfar, Aalim M. Weljie, Karen Kopciuk, Hans J. Vogel, Oliver F. Bathe
  • Patent number: 8999897
    Abstract: The use of a tag moiety comprising a biotinylation domain, such as biotin carboxyl carrier protein (BCCP), as a protein folding marker and protein solubility enhancer in the orientated surface capture of products of heterologously expressed genes is described. Methods for increasing the solubility of proteins and determining the folded state of a protein are also disclosed. The uses and methods of the invention can be carried out in a multiplexed manner on more than one protein in the formation of libraries. In addition the nucleic acid molecule encoding the biotinylation domain of the tag moiety can be used to increase the proportion of clones in a library that express the protein of interest.
    Type: Grant
    Filed: January 29, 2003
    Date of Patent: April 7, 2015
    Assignee: Sense Proteomic Limited
    Inventors: Mitali Samaddar, Jonathan Michael Blackburn, Darren James Hart, Michael Richard Dyson
  • Publication number: 20150094232
    Abstract: The present invention generally pertains to methods and kits for managing the variation in spectroscopic intensity measurements through the use of a reference component. The reference component may comprise a reference spectroscopic substance and may be contained together with a sample of interest in a sample to be tested, wherein the sample of interest may comprise a sample spectroscopic substance. Each sample to be tested may be uniquely identified and, hence, “barcoded” by combinations of different colors and concentrations of spectroscopic substances, contained therein.
    Type: Application
    Filed: March 28, 2012
    Publication date: April 2, 2015
    Applicant: GnuBIO, Inc.
    Inventors: Adam Abate, John Lawrence Emhoff, Tony Hung, Adnan Moez Esmail, Sepehr Kiani, Pascaline Mary
  • Publication number: 20150094233
    Abstract: The present invention provides a compound represented by the formula (I): or a salt thereof; and a method of quantitatively analyzing an amino group-containing target substance, including labeling a target substance in samples by using, as a labeling compound, two or more of such compounds having a mutually different mass due to isotope labeling, to confer a mass difference to the target substance between two or more samples, and the like.
    Type: Application
    Filed: March 28, 2013
    Publication date: April 2, 2015
    Inventors: Shigeru Matsukawa, Kazumi Narita, Yasushi Arakawa, Haruki Shimodaira
  • Publication number: 20150094234
    Abstract: This invention relates to a method for characterizing and/or determining a samples, particularly in the aid of lanthanide(III) ions and ligands including an aromatic structure and 2-5 chelating heteroatoms. The invention relates also to an array for characterizing and/or determining a samples utilizing the properties of lanthanide(III) chelates.
    Type: Application
    Filed: April 23, 2013
    Publication date: April 2, 2015
    Inventors: Harri Härmä, Sari Pihlasalo
  • Publication number: 20150087529
    Abstract: Systems, methods, and apparatuses are provided for diagnosing auto-immune diseases such as systemic lupus erythematosus (SLE) based on the sizes, methylation levels, and/or genomic characteristics of circulating DNA molecules. Patients provide blood or other tissue samples containing cell-free nucleic molecules for analysis. Massively parallel and/or methylation-aware sequencing can be used to determine the sizes and methylation levels of individual DNA molecules and identify the number of molecules originating from different genomic regions.
    Type: Application
    Filed: September 19, 2014
    Publication date: March 26, 2015
    Inventors: Yuk-Ming Dennis LO, Rossa Wai Kwun Chiu, Rebecca Wing Yan Chan, Lai Shan Tam
  • Publication number: 20150087554
    Abstract: A method for the diagnosis of the probability of Alzheimer's disease in a subject patient comprising the step of determining the presence or absence of at least three polypeptide markers in a sample, wherein the at least three polypeptide markers are at least three different markers selected from markers 1 to 50 (frequency markers) and markers 51 to 279 (amplitude markers), wherein the diagnosis is based on a comparison and ranking of the frequency and/or amplitude of the at least three polypeptide markers to the same at least three polypeptide markers obtained from control subjects with and without Alzheimer's disease.
    Type: Application
    Filed: September 15, 2014
    Publication date: March 26, 2015
    Inventor: Harald Mischak
  • Publication number: 20150087551
    Abstract: Apparatus, systems, and methods for detecting, screening and sampling of cells are disclosed.
    Type: Application
    Filed: July 22, 2014
    Publication date: March 26, 2015
    Inventor: Rolf U. Halden