By Measuring A Physical Property (e.g., Mass, Etc.) Patents (Class 506/12)
  • Publication number: 20150105296
    Abstract: Using NMR/MS based metabonomics and targeted lipidomics approaches the inventors have explored the metabolic phenotypes of aging and longevity in a cohort compromising centenarians, elderly and young adults. The inventors have identified biomarkers for a reduced risk of developing ageing related chronic inflammatory disorders and propose a method of diagnosing a lifestyle that allows delaying and/or avoiding ageing related chronic inflammatory disorders using hydroxy-sphingomyelin SM-OH-22:1 as biomarker.
    Type: Application
    Filed: September 19, 2014
    Publication date: April 16, 2015
    Inventors: Sebastiano Collino, Ivan Montoliu Roura, Francois-Pierre Martin, Philippe Alexandre Guy, Serge Andre Dominique Rezzi
  • Publication number: 20150105291
    Abstract: It is intended to evaluate an ischemic heart disease with high accuracy by convenient operation. The method for evaluating an ischemic heart disease according to the present invention comprises the steps of: assaying complement factor H and/or complement factor D in a sample derived from the blood of a test subject; and comparing the concentration of the complement factor H and/or the concentration of the complement factor D assayed in the preceding step with a reference value(s), wherein it is determined that the seriousness of the ischemic heart disease is high when the concentration falls below the reference value.
    Type: Application
    Filed: October 15, 2014
    Publication date: April 16, 2015
    Inventors: Hiroko HANZAWA, Takeshi SAKAMOTO, Yuji KUGE
  • Publication number: 20150105297
    Abstract: A micro-fluidic device for mapping a DNA or RNA strand labeled at a plurality of specific sites with labels suitable for generating a detection signal when interacting with a detector element, the device comprising: a micro-fluidic channel; and a plurality of detector elements for detecting the labels by acquiring the detection signals, the detector elements being positioned longitudinally along the micro-fluidic channel, each detector element having a width, successive detector elements being separated by an inter-detector gap having a width, wherein the widths of at least two of the detector elements are different and/or wherein the widths at least two of the inter-detector gaps are different.
    Type: Application
    Filed: October 13, 2014
    Publication date: April 16, 2015
    Applicants: Katholieke Universiteit Leuven, KU LEUVEN R&D, IMEC VZW
    Inventors: Tim Stakenborg, Robert Neely, Pol Van Dorpe, Johan Hofkens
  • Publication number: 20150104440
    Abstract: The current disclosure reveals a complex regulatory pattern between miR-31 and AR, indicating that miR-31 plays a key role in prostate cancer development and progression. Another aspect of the current disclosure shows that miR-31 directly targets and destabilizes AR mRNA through interaction with the AR mRNA coding sequence showing that miR-31, or a fragment thereof has the ability to act as a novel therapeutic agent in treating cancer. The current disclosure also shows that AR indirectly represses miR-31 expression by binding to the miR-31 promoter region and modulating methyltransferase activity. Another aspect of the current disclosure shows that miR-31 indirectly modulates AR activity by modulating regulators of cell cycle progression. The disclosure further provides an isolated nucleic acid that modulates the activity of the androgen receptor in a cell.
    Type: Application
    Filed: April 12, 2013
    Publication date: April 16, 2015
    Applicant: CORNELL UNIVERSITY
    Inventors: Pei-Chun Lin, Mark A. Rubin
  • Publication number: 20150105293
    Abstract: The inventors have proposed a novel panel of human plasma protein biomarkers for diagnosing hepatic fibrosis and cirrhosis. Presently there is no reliable non-invasive way of assessing liver fibrosis. A 2D-PAGE based proteomics study was used to identify potential fibrosis biomarkers. Plasma from patients with hepatic cirrhosis induced by infection with the hepatitis C virus (HCV) were analysed. Several proteins associated with liver scarring and potentially also related to viral infection were identified. These proteins include 14-3-3 protein zeta/delta, adiponectin, afamin, alpha-1-antitrypsin, alpha-2-HS-glycoprotein, apolipoprotein C-M, apolipoprotein E, C4b-binding protein beta chain, intact/cleaved complement C3dg, corticosteroid-binding globulin, fibrinogen gamma chain, beta haptoglobin at pH 5.46-5.
    Type: Application
    Filed: October 23, 2014
    Publication date: April 16, 2015
    Applicant: The Chancellor, Masters and Scholars of The University of Oxford
    Inventors: Bevin Gangadharan, Nicole Titzmann, Raymond A. Dwek
  • Publication number: 20150099669
    Abstract: The presently disclosed subject matter provides methods using mass spectrometry for direct profiling of N-linked glycans from a biological sample. In addition, the embodiments of the present invention also disclose novel methods, known as targeted analyte detection (TAD), for improving the detection limit of MALDI-MS. These methods take advantage of the carrier effect of the added standard analytes, which occurs due to the generic sigmoidal shape of the calibration curve. The functionality of TAD depends on the relative enhancement of sensitivity over the increase of the standard deviation at the analysis of target analytes with spiking in exogenous concentration. At certain ranges of exogenous concentration, the increment in the sensitivity overcomes the standard deviation, resulting in an improved LOD. Theoretically, exogenous concentrations approximately at 1 LODorig would generate the optimum LOD improvement.
    Type: Application
    Filed: May 23, 2013
    Publication date: April 9, 2015
    Inventors: Hui Zhang, Xingde Li, Shadi Toghi Eshghi
  • Publication number: 20150099668
    Abstract: This document provides methods and materials for assessing metabolites in a sample from a mammal (e.g., human) for determining the nature and extent of colorectal cancer (CRC) metastasis. For example, the document relates to the diagnosis, staging and prognosis of CRC in a mammal.
    Type: Application
    Filed: May 14, 2013
    Publication date: April 9, 2015
    Applicant: UTI LIMITED PARTNERSHIP
    Inventors: Farshad Farshidfar, Aalim M. Weljie, Karen Kopciuk, Hans J. Vogel, Oliver F. Bathe
  • Publication number: 20150099664
    Abstract: A method for characterizing the risk a subject will develop an autoimmune and/or alloimmune disease following tissue transplant includes obtaining a biological sample from the subject, wherein the subject has received the tissue transplant determining in the biological sample a level of at least one protein selected from Tables 1-4, comparing the measured level of the at least one protein to a control value, and characterizing a subject as at greater risk of developing an autoimmune disease and/or alloimmune disease if the level of at least one protein determined is increased or decreased compared to the control value.
    Type: Application
    Filed: December 15, 2014
    Publication date: April 9, 2015
    Inventors: Mark Chance, Kenneth Cooke, Daniela Schlatzer
  • Patent number: 8999897
    Abstract: The use of a tag moiety comprising a biotinylation domain, such as biotin carboxyl carrier protein (BCCP), as a protein folding marker and protein solubility enhancer in the orientated surface capture of products of heterologously expressed genes is described. Methods for increasing the solubility of proteins and determining the folded state of a protein are also disclosed. The uses and methods of the invention can be carried out in a multiplexed manner on more than one protein in the formation of libraries. In addition the nucleic acid molecule encoding the biotinylation domain of the tag moiety can be used to increase the proportion of clones in a library that express the protein of interest.
    Type: Grant
    Filed: January 29, 2003
    Date of Patent: April 7, 2015
    Assignee: Sense Proteomic Limited
    Inventors: Mitali Samaddar, Jonathan Michael Blackburn, Darren James Hart, Michael Richard Dyson
  • Publication number: 20150094234
    Abstract: This invention relates to a method for characterizing and/or determining a samples, particularly in the aid of lanthanide(III) ions and ligands including an aromatic structure and 2-5 chelating heteroatoms. The invention relates also to an array for characterizing and/or determining a samples utilizing the properties of lanthanide(III) chelates.
    Type: Application
    Filed: April 23, 2013
    Publication date: April 2, 2015
    Inventors: Harri Härmä, Sari Pihlasalo
  • Publication number: 20150094232
    Abstract: The present invention generally pertains to methods and kits for managing the variation in spectroscopic intensity measurements through the use of a reference component. The reference component may comprise a reference spectroscopic substance and may be contained together with a sample of interest in a sample to be tested, wherein the sample of interest may comprise a sample spectroscopic substance. Each sample to be tested may be uniquely identified and, hence, “barcoded” by combinations of different colors and concentrations of spectroscopic substances, contained therein.
    Type: Application
    Filed: March 28, 2012
    Publication date: April 2, 2015
    Applicant: GnuBIO, Inc.
    Inventors: Adam Abate, John Lawrence Emhoff, Tony Hung, Adnan Moez Esmail, Sepehr Kiani, Pascaline Mary
  • Publication number: 20150094233
    Abstract: The present invention provides a compound represented by the formula (I): or a salt thereof; and a method of quantitatively analyzing an amino group-containing target substance, including labeling a target substance in samples by using, as a labeling compound, two or more of such compounds having a mutually different mass due to isotope labeling, to confer a mass difference to the target substance between two or more samples, and the like.
    Type: Application
    Filed: March 28, 2013
    Publication date: April 2, 2015
    Inventors: Shigeru Matsukawa, Kazumi Narita, Yasushi Arakawa, Haruki Shimodaira
  • Publication number: 20150087525
    Abstract: The present invention relates in general to the field of colorectal cancer detection, and more particularly, to plasma microRNAs for the detection of early colorectal cancer.
    Type: Application
    Filed: October 20, 2012
    Publication date: March 26, 2015
    Applicant: HOSPITAL CLINIC DE BARCELONA
    Inventors: I Cos Meritxell Gironella, Juan Jose Lozano Salvatella, i Garangou Antoni Castells, Maria Dolores Giraldez
  • Publication number: 20150087529
    Abstract: Systems, methods, and apparatuses are provided for diagnosing auto-immune diseases such as systemic lupus erythematosus (SLE) based on the sizes, methylation levels, and/or genomic characteristics of circulating DNA molecules. Patients provide blood or other tissue samples containing cell-free nucleic molecules for analysis. Massively parallel and/or methylation-aware sequencing can be used to determine the sizes and methylation levels of individual DNA molecules and identify the number of molecules originating from different genomic regions.
    Type: Application
    Filed: September 19, 2014
    Publication date: March 26, 2015
    Inventors: Yuk-Ming Dennis LO, Rossa Wai Kwun Chiu, Rebecca Wing Yan Chan, Lai Shan Tam
  • Publication number: 20150087554
    Abstract: A method for the diagnosis of the probability of Alzheimer's disease in a subject patient comprising the step of determining the presence or absence of at least three polypeptide markers in a sample, wherein the at least three polypeptide markers are at least three different markers selected from markers 1 to 50 (frequency markers) and markers 51 to 279 (amplitude markers), wherein the diagnosis is based on a comparison and ranking of the frequency and/or amplitude of the at least three polypeptide markers to the same at least three polypeptide markers obtained from control subjects with and without Alzheimer's disease.
    Type: Application
    Filed: September 15, 2014
    Publication date: March 26, 2015
    Inventor: Harald Mischak
  • Publication number: 20150087553
    Abstract: The present invention is directed to methods for predicting a pregnant woman's risk of developing early-onset preeclampsia or late-onset preeclampsia. The methods are based on measuring one or more metabolites obtained from a pregnant woman's bodily fluid, such as blood or urine, which were found to be predictive of early-onset preeclampsia and late-onset preeclampsia.
    Type: Application
    Filed: April 12, 2013
    Publication date: March 26, 2015
    Inventors: Ray O. Bahado-Singh, Kypros Nicolaides
  • Publication number: 20150087728
    Abstract: Methods are provided for identifying biomarker proteins that exhibit differential expression in subjects with a first lung condition versus healthy subjects or subjects with a second lung condition. Also provided are compositions comprising these biomarker proteins and methods of using these biomarker proteins or panels thereof to diagnose, classify, and monitor various lung conditions. The methods and compositions provided herein may be used to diagnose or classify a subject as having lung cancer or a non-cancerous condition, and to distinguish between different types of cancer (e.g., malignant versus benign, SCLC versus NSCLC).
    Type: Application
    Filed: September 19, 2014
    Publication date: March 26, 2015
    Inventors: Paul Edward Kearney, Clive Hayward, Xiao-Jun Li
  • Publication number: 20150087551
    Abstract: Apparatus, systems, and methods for detecting, screening and sampling of cells are disclosed.
    Type: Application
    Filed: July 22, 2014
    Publication date: March 26, 2015
    Inventor: Rolf U. Halden
  • Publication number: 20150080257
    Abstract: A method and evaluation kit are provided, in which a high-capacity urate transporter is identified to assist in the early treatment and prevention of urate transport-related disease and inflammation-related disease. The method can include a step for detecting variations in genes that encode ABCG2 protein. When a subject has an SNP of V12M, R113X, Q126X, Q141K, F2085, G268R, E334X, S441N, L447V, S486N, F506SfsX4, R575X, and/or C608X, it can be concluded that the subject has a factor that is capable of inducing urate transport failure, or a state or disease attributable to that failure. When a subject has an SNP of V12M, it can be concluded that, unlike the other SNPs, there is a possibility that the subject does not possess such a factor because, although this variation itself does not lead to a change in urate transport capability, said variation is related to linkage disequilibrium with other SNPs.
    Type: Application
    Filed: November 16, 2014
    Publication date: March 19, 2015
    Inventors: HIROTAKA MATSUO, Nariyoshi Shinomiya, Takahiro Nakamura, Tappei Takada, Hiroshi Suzuki, Yuki Ikebuchi, Kousei Ito, Kimiyoshi Ichida
  • Publication number: 20150080248
    Abstract: The invention generally relates to methods for isolating proteins. In certain aspects, methods of the invention involve preparing a plurality of sample preparations, each preparation including one or more intact cells. A capture unit is introduced to a plurality of the preparations. The capture unit includes a member that transiently interacts with one or more proteins in the cells and a reactive functional group. The sample preparations are incubated, and a reaction is initiated at a different time in a plurality of the preparation. In this manner, a protein within the cell that specifically interacts with the member of the capture unit becomes bound to the capture unit via the reactive functional group to form protein/capture unit complexes. The cells are lysed and the protein/capture unit complexes are isolated.
    Type: Application
    Filed: March 13, 2013
    Publication date: March 19, 2015
    Inventors: Weiguo Andy Tao, Li Yang
  • Publication number: 20150080263
    Abstract: The present invention is directed to methods for predicting a pregnant woman's risk of carrying a fetus with Down syndrome (Trisomy 21) or Trisomy 18. The methods are based on measuring one or more metabolites obtained from a pregnant woman's bodily fluid, such as blood or urine, and found to be predictive of Trisomy 21 or Trisomy 18.
    Type: Application
    Filed: April 12, 2013
    Publication date: March 19, 2015
    Inventors: Ray O. Bahado-Singh, Kypros Nicolaides
  • Publication number: 20150080264
    Abstract: The present invention generally relates to the field of biomarkers. In particular, the present invention relates to biomarkers such as PC-O 44:6 that can be used, for example for detecting and/or quantifying visceral adiposity and/or changes in visceral adiposity. This biomarker may also be used to diagnosing the effect of a change in lifestyle on visceral adiposity in a subject.
    Type: Application
    Filed: November 25, 2014
    Publication date: March 19, 2015
    Inventors: Francois-Pierre Martin, Ivan Montoliu Roura, Philippe Alexandre Guy, Serge Andre Dominique Rezzi
  • Publication number: 20150072888
    Abstract: The present invention relates to the use of N-linked glycan profiles of blood or blood component proteins as biomarkers for diagnosing diabetes mellitus and for monitoring the efficacy of anti-diabetic therapy. Specifically, the present invention relates to detecting changes in the amounts of N-linked glycans as diagnostic biomarkers for diabetes mellitus and as indicators of the efficacy of anti-diabetic therapy over time.
    Type: Application
    Filed: April 19, 2013
    Publication date: March 12, 2015
    Inventors: Hidehisa Asada, Diane McCarthy, Yoshiaki Miura, Taku Nakahara
  • Publication number: 20150072895
    Abstract: The current disclosure provides for specific peptides, and derived ionization characteristics of the peptides, from the KRT5, KRT7, NapsinA, TTF1, TP63, and/or MUC1 proteins that are particularly advantageous for quantifying the KRT5, KRT7, NapsinA, TTF1, TP63, and/or MUC1 proteins directly in biological samples that have been fixed in formalin by the method of Selected Reaction Monitoring (SRM) mass spectrometry, or what can also be termed as Multiple Reaction Monitoring (MRM) mass spectrometry. Such biological samples are chemically preserved and fixed wherein said biological sample is selected from tissues and cells treated with formaldehyde containing agents/fixatives including formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (FFPE) tissue/cells, FFPE tissue blocks and cells from those blocks, and tissue culture cells that have been formalin fixed and or paraffin embedded.
    Type: Application
    Filed: November 17, 2014
    Publication date: March 12, 2015
    Inventors: David B. KRIZMAN, Wei-Li LIAO, Sheeno THYPARAMBIL, Todd HEMBROUGH
  • Publication number: 20150072043
    Abstract: Using NMR/MS based metabonomics and targeted lipidomics approaches the inventors have explored the metabolic phenotypes of aging and longevity in a cohort compromising centenarians, elderly and young adults. The inventors have identified biomarkers for a reduced risk of developing ageing related chronic inflammatory disorders and propose a method of diagnosing a lifestyle that allows delaying and/or avoiding ageing related chronic inflammatory disorders using 9-oxo-HODE as biomarker.
    Type: Application
    Filed: September 19, 2014
    Publication date: March 12, 2015
    Inventors: Sebastiano Collino, Ivan Montoliu Roura, Francois-Pierre Martin, Philippe Alexandre Guy, Serge Andre Dominique Rezzi
  • Publication number: 20150065365
    Abstract: An apparatus is provided for sensing an analyte in a fluid.
    Type: Application
    Filed: May 12, 2014
    Publication date: March 5, 2015
    Applicant: Invoy Technologies, L.L.C
    Inventor: Lubna Ahmad
  • Publication number: 20150065390
    Abstract: Examples of integrated sensors are disclosed herein. An example of an integrated sensor includes a substrate and a sensing member formed on a surface of the substrate. The sensing member includes collapsible signal amplifying structures and an area surrounding the collapsible signal amplifying structures that enables self-positioning of droplets exposed thereto toward the collapsible signal amplifying structures.
    Type: Application
    Filed: April 20, 2012
    Publication date: March 5, 2015
    Inventors: Alexandre M. Bratkovski, Zhiyong Li, Wei Wu, Min Hu, R. Stanley Williams, Ansoon Kim
  • Publication number: 20150065373
    Abstract: A novel method that enables prostate cancer testing that is noninvasive and more accurate than conventional methods is disclosed. The present inventors intensively analyzed urine samples from prostate cancer patients, and non-cancer subjects, who are free of prostate cancer, and, as a result, newly discovered urinary peptides that can be used as indicators in prostate cancer testing. Use of these urinary peptides as indicators enables various prostate cancer-related tests including detection of prostate cancer, discrimination between prostate cancer and benign prostatic hyperplasia, monitoring of a therapeutic effect of prostate cancer therapy and monitoring of postoperative recurrence.
    Type: Application
    Filed: March 28, 2013
    Publication date: March 5, 2015
    Applicant: KYOTO UNIVERSITY
    Inventors: Kenji Nakayama, Kazuharu Shimizu, Jun Utsumi, Takahiro Inoue, Osamu Ogawa
  • Publication number: 20150065391
    Abstract: Method for diagnosis of heart failure, comprising the step of determining the presence or absence or amplitude of at least three polypeptide markers in a urine sample, the polypeptide markers being selected from the markers characterized in Table 1 by values for the molecular masses and the migration time.
    Type: Application
    Filed: January 30, 2013
    Publication date: March 5, 2015
    Inventor: Harald Mischak
  • Publication number: 20150065366
    Abstract: Methods for identifying and evaluating biochemical entities useful as biomarkers for bladder cancer, target identification/validation, and monitoring of drug efficacy are provided. Also provided are suites of small molecule entities as biomarkers for bladder cancer.
    Type: Application
    Filed: November 8, 2012
    Publication date: March 5, 2015
    Inventors: Jonathan E. McDunn, Regis Perichon, Bruce Neri, Bryan Wittmann
  • Publication number: 20150057185
    Abstract: Carbon monoxide (CO) is a member of the gasotransmitter family that includes NO and H2S and is implicated in a variety of pathological and physiological conditions. Whereas exogenous therapeutic additions of CO to tissues and whole animals have been well studied, the real-time spatial and temporal tracking of CO at the cellular level remains an open challenge. We now report a new type of turn-on fluorescent probe for selective CO detection by exploiting palladium-mediated carbonylation reactivity. The compounds of the invention are capable of detecting CO both in aqueous buffer and in live cells with high selectivity over a range of biologically relevant reactive small molecules, providing a potentially powerful approach for interrogating its chemistry in biological systems.
    Type: Application
    Filed: August 26, 2013
    Publication date: February 26, 2015
    Inventors: Christopher J. CHANG, Brian William Michel
  • Publication number: 20150051111
    Abstract: A method is described for mass spectrometric analysis of a sample comprising phenolic OH, such as a steroid comprising a phenolic OH, using a quaternary amino oxy Cookson (QAOC) reagent. The QAOC reagent can improve ionization and fragmentation properties of phenolic OH samples, which can thereby improve quantitation and identification. The method can include derivatizing the phenolic OH sample with the QAOC reagent to create an adduct and analyzing the adduct using mass spectrometry. Derivatization of the sample can be a one-step reaction where the QAOC reagent comprises an aminooxy MS tag or can be a multi-step reaction, where the adduct is formed by the reaction of carbonyl substituted PTAD based reagent and the sample followed by combination with an aminooxy MS tag. The sample can also be enriched prior to reacting it with the reagent. The method can also allow for multiplexing.
    Type: Application
    Filed: January 16, 2013
    Publication date: February 19, 2015
    Inventors: Subhakar Dey, Subhasish Purkayastha
  • Publication number: 20150045245
    Abstract: The application discloses new biomarkers or test panels useful in systemic inflammatory conditions such as sepsis; methods for the diagnosis, prediction, prognosis and/or monitoring systemic inflammatory conditions such as sepsis based on measuring said biomarkers or test panels; and related kits and devices.
    Type: Application
    Filed: December 7, 2012
    Publication date: February 12, 2015
    Applicant: BIOCARTIS NV
    Inventors: Griet Vanpoucke, Katleen Verleysen, Yven Van Herrewege
  • Publication number: 20150045256
    Abstract: A method for screening a drug for cytochrome P450 (CYP) induction is provided and can include incubating the drug with a microsome-containing biological sample and then quantitating at least one cytochrome P450 isoform. The isoforms can be selected from 2B6, 3A4, 1A2, and 3A5 isoforms. In some embodiments, the method uses liquid chromatography tandem mass spectrometry (LC-MSMS). A quantitated value can be compared to a threshold value and the drug can be determined to exhibit an acceptable CYP induction potential when the quantitated value does not exceed the threshold value. Isolated peptides are also provided.
    Type: Application
    Filed: August 21, 2014
    Publication date: February 12, 2015
    Inventors: Brian L. Williamson, Subhasish Purkayastha
  • Publication number: 20150031582
    Abstract: A method comprising (a) obtaining one or more biological samples from a subject wherein the subject has undergone an organ transplant; (b) determining the amount and type of one or more volatile organic compounds in the biological sample; and (c) correlating the amount of volatile organic compounds to a degree of transplant rejection. A device comprising a plurality of sensors configured to detect a plurality of volatile organic compounds in a biological sample of a subject having undergone an organ transplant.
    Type: Application
    Filed: July 28, 2014
    Publication date: January 29, 2015
    Applicant: UNIVERSITY OF HOUSTON SYSTEM
    Inventors: Dong Cai, Zhifeng Ren, Xian C. Li, Renard L. Thomas
  • Publication number: 20150031581
    Abstract: The invention relates to the field of diagnostic methods, in particular to detection of biological molecules using mass spectroscopy (MS). Provided is an automated high-throughput method for quantitating a low molecular weight protein-bound biomarker directly in an isolated biological sample, comprising the steps of: (a) automated prepurification of the sample using an effective amount of an acid protease under conditions that allow for digestion of one or more binding proteins; (b) applying said protease-digested sample onto an on-line SPE column to capture at least part of said biomarker, followed by sequential washing of the solid phase; (c) eluting a fraction comprising said biomarker directly onto a liquid chromatography (LC) column comprising an apolar stationary phase and subjecting it to LC-MS or LC-MS-MS measurements to determine the amount of at least one biomarker; and (d) quantitating the biomarker(s).
    Type: Application
    Filed: November 29, 2012
    Publication date: January 29, 2015
    Inventors: Ido Peter Kema, Hermannus Johannes Roelof van Faassen, Bernhard Manz
  • Publication number: 20150031580
    Abstract: A method for quantitative assessment of base excision repair (BER) and nucleotide excision repair (NER) DNA repair capacities of at least one cellular extract, which method entails: a) preparing a range of plasmids with at least one physical or chemical treatment or both and recovering a supercoiled fraction of each of said plasmids, b) characterizing the lesions present on each of the plasmids of the range of plasmids; c) depositing the plasmids of the range of plasmids, and at least one supercoiled control plasmid without lesions onto a single solid support, according to a pre-established configuration A, so as to form a functionalized support divided into different zones A1 to Ax, corresponding to an integer equal to the number of cellular extracts to be simultaneously tested, each zone containing supercoiled plasmids treated with at least one physical or chemical treatment that induces a lesion acted upon by BER mechanisms, and at least one other zone containing supercoiled plasmids treated with at least
    Type: Application
    Filed: July 21, 2014
    Publication date: January 29, 2015
    Applicant: COMMISSARIAT A L'ENERGIE ATOMIQUE
    Inventor: Sylvie SAUVAIGO
  • Publication number: 20150024970
    Abstract: The process for the diagnosis of a renal cell carcinoma comprises the step of determining the presence or absence or amplitude of at least three polypeptide markers in a urine sample, wherein said polypeptide markers are selected from the markers as characterized in Table 1 by molecular masses and migration times.
    Type: Application
    Filed: October 1, 2014
    Publication date: January 22, 2015
    Inventor: Harald Mischak
  • Publication number: 20150024969
    Abstract: A method for determining prognosis, diagnosis and theronosis of a sepsis infection in a patient is disclosed. The method involves measuring the age, mean arterial pressure, hematocrit, patient temperature, and the concentration of one or more metabolites that are predictive of sepsis severity. The method can involve obtaining a blood sample from said patient and determining the concentration of the metabolite in the patient's blood; and then determining the severity of sepsis infection by analyzing the measured values in a weighted logistic regression equation.
    Type: Application
    Filed: September 12, 2012
    Publication date: January 22, 2015
    Applicant: THE CHILDREN'S MERCY HOSPITAL
    Inventors: Raymond Langley, Stephen Kingsmore
  • Publication number: 20150024960
    Abstract: The present invention relates to a biomarker set for diagnosing breast cancer comprising two or more protein markers of: apolipoprotein C1, apolipoprotein (a), neural cell adhesion molecule L1-like protein, carbonic anhydrase 1, and fibronectin; a method for detecting the biomarker set in a blood sample through a multiple reaction monitoring; a kit for diagnosing breast cancer comprising antibodies specific to each of the proteins of the biomarker set; and a method for detecting proteins of the marker set in a blood sample through an antigen-antibody binding reaction. The method for detecting the protein marker set in a blood sample by the MRM method or antigen-antibody binding reaction and the diagnostic kit can provide very high accuracy and sensitivity in comparison with the diagnosis method using a single marker and can very conveniently diagnose breast cancer using blood from patients, thereby being effectively used for early diagnosis of breast cancer.
    Type: Application
    Filed: March 8, 2013
    Publication date: January 22, 2015
    Applicants: SNU R&DB FOUNDATION, BIOMEDIENG CO., LTD.
    Inventors: Heon Soo Lee, Hye Seoung Shin, Un-Beom Kang, Dong-Young Noh, Hyeong-Gon Moon
  • Publication number: 20150024968
    Abstract: Apparatus, systems and methods for use in analyzing discrete reactions are provided. The analytical devices of the invention use an array of nanoscale regions (a chip) that has discrete patches of nanoscale regions. The chip mates with a collection device comprising an array of compact lens trains (CLTs) where each of the CLTs corresponds to a single patch of nanoscale regions. Each CLT collects the emitted light from a patch on the chip, collimates the light, performs color separation on the collimated emitted light, and focuses the separated light onto a portion of pixels on the detector below the CLT. Such systems are useful for monitoring many analytical reactions at one time including single molecule sequencing reactions.
    Type: Application
    Filed: May 29, 2014
    Publication date: January 22, 2015
    Applicant: Pacific Biosciences of California, Inc.
    Inventors: Aaron Rulison, Mark McDonald, Paul Lundquist
  • Publication number: 20150018245
    Abstract: Systems and methods for arranging microdevices on an array substrate are described. Each microdevice contains a lipid bilayer region and a patterned magnetic material that has a predetermined preferential axis of magnetization. The microdevices are located at specific locations on the arraying substrates and a magnetic field is used to orient the microdevices to facilitate the study of lipids and lipid-associated moieties. The bilayer region can also contain a fluorescent reagent, which can be monitored during exposure to an excitation source. The fluorescent reagent can also be photobleached and monitored for fluorescence recovery.
    Type: Application
    Filed: March 21, 2013
    Publication date: January 15, 2015
    Applicant: ARRAYOMICS, INC.
    Inventors: Edward T. Castellana, Bao Nguyen, David Rothwarf
  • Publication number: 20150018244
    Abstract: The present invention pertains to the field of diagnostics for hyperthyroidism and toxicological assessments for risk stratification of chemical compounds. Specifically, it relates to a method for diagnosing hyperthyroidism. It also relates to a method for determining whether a compound is capable of inducing such hyperthyroidism in a subject and to a method of identifying a drug for treating hyperthyroidism. Furthermore, the present invention relates to a device and a kit for diagnosing hyperthyroidism.
    Type: Application
    Filed: March 7, 2013
    Publication date: January 15, 2015
    Applicant: BASF SE
    Inventors: Tilmann B. Walk, Bennard van Ravenzwaay, Werner Mellert, Eric Fabian, Volker Strauss, Hennicke Kamp, Jan C. Wiemer, Ralf Looser, Michael Manfred Herold, Alexandre Prokoudine
  • Publication number: 20150018246
    Abstract: The invention relates to a method for diagnosing vascular diseases, comprising the step in which the presence, absence or amplitude of at least three polypeptide markers is determined in a urine sample, wherein the polypeptide markers are selected from the markers characterized in table 1 by values for the molecular masses and the migration time.
    Type: Application
    Filed: September 26, 2014
    Publication date: January 15, 2015
    Inventor: Harald Mischak
  • Publication number: 20150018237
    Abstract: The present invention pertains to the field of diagnostics for bone disorders and toxicological assessments for risk stratification of chemical compounds. Specifically, it relates to a method for diagnosing a bone disorder. It also relates to a method for determining whether a compound is capable of inducing such a bone disorder in a subject and to a method of identifying a drug for treating a bone disorder. Furthermore, the present invention relates to a device and a kit for diagnosing a bone disorder.
    Type: Application
    Filed: February 15, 2013
    Publication date: January 15, 2015
    Applicant: BASF SE
    Inventors: Tilmann B. Walk, Bennard van Ravenzwaay, Werner Mellert, Eric Fabian, Volker Strauss, Hennicke Kamp, Jan C. Wiemer, Ralf Looser, Michael Manfred Herold, Alexandre Prokoudine
  • Publication number: 20150011423
    Abstract: The present invention pertains to the field of diagnostics for kidney toxicity and toxicological assessments for risk stratification of chemical compounds. Specifically, it relates to a method for diagnosing kidney toxicity. It also relates to a method for determining whether a compound is capable of inducing such kidney toxicity in a subject and to a method of identifying a drug for treating kidney toxicity. Furthermore, the present invention relates to a device and a kit for diagnosing kidney toxicity.
    Type: Application
    Filed: September 14, 2012
    Publication date: January 8, 2015
    Applicant: BASF SE
    Inventors: Hennicke Kamp, Tilmann B. Walk, Bennard van Ravenzwaay, Werner Mellert, Eric Fabian, Volker Strauss, Jan C. Wiemer, Ralf Looser, Michael Manfred Herold, Alexandre Prokoudine
  • Publication number: 20150011425
    Abstract: The present invention relates to methods and arrays for use in high resolution imaging of individual nucleic acid molecules and chromatin fragments, including native chromatin fragments. In one aspect, the present invention relates to a chromatin array that includes a transfer platform having a support and a transfer surface layered on the support. The chromatin array also includes a plurality of elongated individual native chromatin fragments coupled to the transfer surface in an orderly pattern suitable for high resolution imaging of the plurality of native chromatin fragments. The native chromatin fragments of the chromatin array include both DNA and histones.
    Type: Application
    Filed: January 28, 2013
    Publication date: January 8, 2015
    Applicant: CORNELL UNIVERSITY
    Inventors: Aline Cerf, Harold G. Craighead, Harvey C. Tian
  • Publication number: 20150011424
    Abstract: The present invention is based on the idea of determining certain molecular lipids from a subject's blood sample, for example from serum or plasma sample, and based on the amounts of the determined lipids determining the amount of liver fat and/or diagnosing NAFLD in the subject. More specifically the subjects with elevated liver fat amount and NAFLD are characterized by elevated triglycerides with low carbon number and double bond content in the blood sample. Lysophosphatidylcholines, ether phospholipids, sphingomyelins and PUFA-containing phospholipids are diminished in the blood samples of subjects with an elevated liver fat amount and NAFLD. The method of the present invention can be further used for monitoring the subject's response to the treatment of NAFLD or to the treatment of lowering of the liver fat amount in the subject.
    Type: Application
    Filed: January 30, 2013
    Publication date: January 8, 2015
    Inventors: Matej Oresic, Tuulia Hyötyläinen, Gopalacharyulu Peddinti, Sandra Castillo, Hannele Yki-Järvinen
  • Publication number: 20150011426
    Abstract: A novel non-enzymatic assay for lipid modulating drugs is being described for biological samples. For the first time non-enzymatic mechanism of statin drugs in modulating lipid aggregates and forming lipid particles is being shown. A simple flow cytometer based testing for detection of lipid particles, the effect of lipid modulating drug such as statin in vitro and testing for efficacy of the drug for an individual, dosage adjustment and drug discovery mechanism is being described. The lipid modulating drugs either individually or in combination can be discovered, optimized or efficacy tested using this assay. A novel statin induced lipid particle formation described can be used for diagnosis, personalized medicine and biomarker identification as well.
    Type: Application
    Filed: September 4, 2014
    Publication date: January 8, 2015
    Applicant: PLAXGEN INC
    Inventor: Shanmugavel Madasamy
  • Publication number: 20150005196
    Abstract: A method and apparatus for determining the presence or absence of microorganisms in a liquid sample. A vessel with an electrode disposed therein receives a volume of liquid to be tested. A second electrode is also provided, both electrodes in physical contact with the liquid sample. A time varying signal is applied to one electrode, and the other electrode is coupled to a phase sensitive signal detector. The phase sensitive signal detector determines a frequency at which an out of phase signal amplitude is zero. This zero-crossing frequency is used as a baseline, and changes in the zero-crossing frequency are an indication of microbial growth.
    Type: Application
    Filed: February 14, 2013
    Publication date: January 1, 2015
    Applicant: Becton, Dickinson and Company
    Inventor: Klaus W. Berndt