Rna Or Dna Which Encodes Proteins (e.g., Gene Library, Etc.) Patents (Class 506/17)
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Publication number: 20110257041Abstract: Aspects of the invention relate to reconfigurable chassis that allow for rapid construction and optimization of biocircuits.Type: ApplicationFiled: November 15, 2010Publication date: October 20, 2011Applicant: Massachusetts Institute of TechnologyInventors: Jonathan William Babb, Ron Weiss, Thomas Knight, Adam Rubin
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Publication number: 20110257034Abstract: Closures for containers and methods for using same are provided. In a general embodiment/the present disclosure provides a closure having a top portion (12), a bottom portion (14) and a side portion (16), an aperture (18) extending though the closure, a projection (20) extending from the closure and at least two rib members (36) on an interior of the projection. The projection may also include a cover (22). In another embodiment, a method for using a closure includes inserting a. spike member into a projection, piercing a membrane that hermetically seals a medical container, pushing rib members within the projection to center the spike member inserted into the projection, and tearing the membrane to create a vent hole in the membrane.Type: ApplicationFiled: October 13, 2009Publication date: October 20, 2011Applicants: CORNELL UNIVERSITY, THE TRUSTEES OF PRINCETON UNIVERSITY, SLOAN-KETTERING INSTITUTE FOR CANCER RESEARCH, UNIVERSITY OF MEDICINE AND DENTISTRY OF NEW JERSEYInventors: Francis Barany, Owen Parker, Manny D. Bacolod, Sarah F. Giardina, Yu-wei Cheng, Daniel A. Notterman, Gunter S. Schemmann, Philip B. Paty, Monib Zirvi
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Publication number: 20110251108Abstract: The invention provides novel polynucleotides and polypeptides encoded by such polynucleotides and mutants or variants thereof that correspond to a novel human secreted stem cell growth factor-like polypeptides. Other aspects of the invention include vectors containing processes for producing novel human secreted stem cell growth factor-like polypeptides, and antibodies specific for such polypeptides.Type: ApplicationFiled: April 5, 2011Publication date: October 13, 2011Inventor: Y. Tom Tang
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Publication number: 20110251107Abstract: The present invention provides methods for assaying binding of compounds to G-quadruplex structures. Also provided are methods for screening candidate compounds for use as modulators of G-quadruplex activity, and methods for screening candidate compounds for telomerase inhibitory activity. The invention further provides novel compounds useful in the assays of the invention.Type: ApplicationFiled: July 12, 2010Publication date: October 13, 2011Inventors: Lesley DAVENPORT, Yossry HUSSEIN
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Publication number: 20110244471Abstract: According to one embodiment, a method of screening an enhancer and/or a promoter, includes culturing a host cell into which an amplifiable vector is introduced, extracting the vector from the host cell and obtaining the DNA fragment from the extracted vector, wherein the vector includes a DNA fragment to be determined, a gene that is functionally linked downstream of the DNA fragment and encodes a protein to initiate self-replication, and a gene that encodes a replication origin sequence.Type: ApplicationFiled: September 21, 2010Publication date: October 6, 2011Inventors: EIICHI AKAHOSHI, MITSUKO ISHIHARA
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Publication number: 20110244459Abstract: Methods for identifying ERBB2 (also named HER2) alteration in tumors, in particular cancer, based on the analysis of the expression of at least three genes of the ERBB2 amplicon located within less than one megabase on either side of ERBB2, and eventually of the gene corresponding to the Affymetrix probeset 234046_at (SEQ ID NO: 31), as well as a poynucleotide library useful for the molecular characterization of a cancer including polynucleotide sequences for detecting the genes, and a kit including the library.Type: ApplicationFiled: December 9, 2009Publication date: October 6, 2011Applicants: IPSOGEN, INSERM-INSTITUT NATIONAL DE LA SANTE ET DE LA rECHERCHE MEDICALE, INSTITUT PAOLI-CALMETTESInventors: Francois Bertucci, Rebecca Tagett, Sabrina Carpentier
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Publication number: 20110245108Abstract: Universal antibody libraries are described which are synthetic and derived from expressed human antibody sequences selected accordingly to certain criteria, for example, that the sequences are derived from naturally-occurring antibodies expressed in response to a certain antigen class (e.g., small molecule, polysaccharide, peptide, or protein) and having CDR regions engineered for optimal diversity. Methods for making and screening such libraries for isolating therapeutics suitable for treating disease are also disclosed.Type: ApplicationFiled: July 6, 2005Publication date: October 6, 2011Inventors: Roberto Crea, Arvind Rajpal, Guido Cappuccilli, Randy Shen, Toshihiko Takeuchi
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Publication number: 20110243925Abstract: The present invention relates to a method for providing a gene expression profile being predictive for the specific response of an individual tumor to a pharmaceutically effective compound, the use thereof, a microarray wherein the nucleotide sequences attached to the substrate consist of nucleotide sequences corresponding to the predictive genes of said gene expression profile, and a diagnostic kit containing said microarray.Type: ApplicationFiled: April 13, 2006Publication date: October 6, 2011Applicant: ONCOTEST GMBHInventors: Niko Bausch, Heinz-Herbert Fiebig, André Korrat, Martina Maurer, Thomas Metz, Julia Schüler
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MARKER FOR DIAGNOSIS OF EXPOSURE TO ELECTROMAGNETIC RADIATION AND DIAGNOSTIC KIT COMPRISING THE SAME
Publication number: 20110237454Abstract: Disclosed are a composition for the diagnosis of exposure to electromagnetic radiation, comprising an agent capable of measuring the expression level of the diagnostic marker, a diagnosis kit comprising the same, a method for detecting the diagnostic marker, and a method for the diagnosis of exposure to electromagnetic radiation. The diagnostic markers are very useful for monitoring and diagnosing exposure to electromagnetic fields, and can be used as instruments by which physiological mechanisms incurred upon electromagnetic radiation exposure are examined.Type: ApplicationFiled: February 24, 2011Publication date: September 29, 2011Applicants: SNU R&DB FOUNDATION, ELECTRONICS AND TELECOMMUNICATIONS RESEARCH INSTITUTEInventors: Woong Yang Park, Ae Kyung Park, Chang Nim Im, Sook Ik Jeon -
MARKER FOR DIAGNOSIS OF EXPOSURE TO ELECTROMAGNETIC RADIATION AND DIAGNOSTIC KIT COMPRISING THE SAME
Publication number: 20110237452Abstract: Disclosed are a composition for the diagnosis of exposure to electromagnetic radiation, comprising an agent capable of measuring the expression level of the diagnostic marker, a diagnosis kit comprising the same, a method for detecting the diagnostic marker, and a method for the diagnosis of exposure to electromagnetic radiation. The diagnostic markers are very useful for monitoring and diagnosing exposure to electromagnetic fields, and can be used as instruments by which physiological mechanisms incurred upon electromagnetic radiation exposure are examined.Type: ApplicationFiled: February 24, 2011Publication date: September 29, 2011Applicants: SNU R&DB FOUNDATION, ELECTRONICS AND TELECOMMUNICATIONS RESEARCH INSTITUTEInventors: Woong Yang Park, Ae Kyung Park, Chang Nim Im, Sook Ik Jeon -
MARKER FOR DIAGNOSIS OF EXPOSURE TO ELECTROMAGNETIC RADIATION AND DIAGNOSTIC KIT COMPRISING THE SAME
Publication number: 20110237453Abstract: Disclosed are a composition for the diagnosis of exposure to electromagnetic radiation, comprising an agent capable of measuring the expression level of the diagnostic marker, a diagnosis kit comprising the same, a method for detecting the diagnostic marker, and a method for the diagnosis of exposure to electromagnetic radiation. The diagnostic markers are very useful for monitoring and diagnosing exposure to electromagnetic fields, and can be used as instruments by which physiological mechanisms incurred upon electromagnetic radiation exposure are examined.Type: ApplicationFiled: February 24, 2011Publication date: September 29, 2011Applicant: ELECTRONICS AND TELECOMMUNICATIONS RESEARCH INSTITUTEInventors: Woong Yang Park, Ae Kyung Park, Chang Nim Im, Sook Ik Jeon -
Publication number: 20110230362Abstract: A method for determining the presence of a copy number imbalance in genomic DNA of a test sample is provided. The method can separately measure hybridization of a single test sample to a first hybridization array and hybridization of a plurality of reference samples to a plurality of other, respective test arrays. A determination of copy number can be based on the best fit reference array, relative to the test array. The best fit can be determined based on the closest or most similar signal-to-noise ratio of the measured signals.Type: ApplicationFiled: March 16, 2010Publication date: September 22, 2011Applicant: BLUEGNOME LIMITEDInventors: Andrew CRAIG, Anthony BROWN, Nicholas HAAN
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Patent number: 8021857Abstract: Novel proteins are provided herein, including proteins capable of catalyzing the acetylation of glyphosate and other structurally related proteins. Also provided are novel polynucleotides capable of encoding these proteins, compositions that include one or more of these novel proteins and/or polynucleotides, recombinant cells and transgenic plants comprising these novel compounds, diversification methods involving the novel compounds, and methods of using the compounds. Some of the novel methods and compounds provided herein can be used to render an organism, such as a plant, resistant to glyphosate.Type: GrantFiled: August 3, 2009Date of Patent: September 20, 2011Assignees: Pioneer Hi-Bred International, Inc., E.I. du Pont de Nemours, Verdia Inc.Inventors: Linda A. Castle, Dan Siehl, Lorraine Giver, Jeremy Minshull, Cristina Ivy, Yong Hong Chen, Phillip A. Patten, Nicholas B. Duck, Rebecca Gorton, Billy Fred McCutchen, Roger Kemble
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Publication number: 20110224100Abstract: The invention relates to collections of target-specific designed binding proteins based on armadillo repeat proteins, and to a method of generating them. Designed armadillo repeat proteins are based on consensus sequences of single armadillo repeat units. These repeat proteins can be used as scaffolds for peptide recognition. Such a scaffold provides a binding mode that is in principle the same for every small recognizable unit, e.g. an amino acid or dipeptide, allowing a precise and modular recognition of a peptide in extended conformation. The method allows to generate a series of modules recognizing these simple units, and to combine such building blocks to create a binding site for any desired peptide target without performing additional selections.Type: ApplicationFiled: September 21, 2008Publication date: September 15, 2011Inventors: Fabio Parmeggiani, Riccardo Pellarin, Anders Peter Larsen, Gautham Varadamsetty, Michael Tobias Stumpp, Andreas Plückthun
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Publication number: 20110207624Abstract: A system for performing quality control for nucleic acid sample sequencing is disclosed. The system comprises a set of solid supports, each solid support having attached thereto a plurality of nucleic acid sequences, wherein the set comprises plural groups of solid supports and each group contains solid supports having the same nucleic acid sequences attached thereto. The nucleic acid sequences of each group differ from each other. The nucleic acid sequences are synthetically derived, and the nucleic acids sequences are designed such that the nucleic acid sequences produce a predefined pattern of detectable signals during a sequencing run. A method of preparing a quality control for performing nucleic acid sample sequencing, a method of validating a nucleic acid sequencing instrument during a nucleic acid sequencing experiment, and a method of processing nucleic acid sequencing data during a nucleic acid sequencing experiment are also disclosed.Type: ApplicationFiled: February 18, 2011Publication date: August 25, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Min-Yi Shen, Douglas Greiner
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Publication number: 20110207628Abstract: A dual display phage system for the identification of protein interaction networks and therapeutic targets.Type: ApplicationFiled: June 9, 2009Publication date: August 25, 2011Applicant: UNIVERSITY OF MIAMIInventors: Wei Li, Xiaoyu Jiang, Nora Blanca Caberoy
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Publication number: 20110201525Abstract: Libraries of nucleic acids encoding chimeric binding polypeptides based on plant scaffold polypeptide sequences. Also described are methods for generating the libraries.Type: ApplicationFiled: April 25, 2011Publication date: August 18, 2011Inventor: Jennifer Jones
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Publication number: 20110195862Abstract: Polymer arrays suitable to perform quantitative and qualitative detection as well as sorting of a target molecules and related devices methods and systems.Type: ApplicationFiled: February 8, 2011Publication date: August 11, 2011Inventors: Jennifer PETT-RIDGE, Paul D. Hoeprich, Eoin Brodie, Peter K. Weber
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Publication number: 20110196042Abstract: The invention provides (1) genes differentially expressed in animals administered fatty acid amides that affect one or more of food intake, satiety, lipid metabolism, and fat utilization and (2) compositions and methods relating to the use of the genes to identify new compounds that affect one or more of food intake, satiety, lipid metabolism, and fat utilization.Type: ApplicationFiled: August 10, 2009Publication date: August 11, 2011Inventors: Delphine Tissot-Favre, Frederic Destaillats, Clementine Thabuis, Jean-Charles Martin
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Publication number: 20110189185Abstract: The invention provides an in vitro method for predicting whether a patient would be responsive to a treatment with an anti-HER2 blocking agent, such as trastuzumab, which method comprises determining the expression level of at least 4 genes in a biological sample of said patient, wherein said genes are GPR22, PEX19, GRHL2 and DERL1. The invention further provides a DNA chip for performing such method.Type: ApplicationFiled: June 8, 2009Publication date: August 4, 2011Applicant: Centre Georges Francois LeclercInventors: Sarab Lizard, Romain Boidot, Laurent Arnould, Bruno Coudert, Pierre Fumoleau
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Publication number: 20110190157Abstract: A molecular classification procedure based on activity levels of modules in protein networks, wherein the proteins are biomarkers for chronic lymphocytic leukemia (CLL), and method for use of the subnetworks to distinguish between patients at low or high risk of progression of their disease.Type: ApplicationFiled: August 14, 2009Publication date: August 4, 2011Inventors: Thomas J. Kipps, Zhouxin Shen, Steven P. Briggs, Trey Ideker, Laura Rassenti, Han-Yu Chuang
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Publication number: 20110183863Abstract: The invention provides methods and compositions useful for identifying polypeptides with desired characteristics in vitro.Type: ApplicationFiled: January 19, 2011Publication date: July 28, 2011Applicant: X-BODY BIOSCIENCESInventors: Richard W. WAGNER, Alexander LITOVCHICK
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Publication number: 20110183870Abstract: Gene expression profiles associated with improved or maintained lean body mass or reduced body fat are disclosed. The gene expression profiles were determined in adipose, liver, and muscle tissue of animals subjected to lean-promoting regimens such as consumption of a high protein diet, ingestion of conjugated linolenic acid, and/or increased exercise.Type: ApplicationFiled: August 10, 2009Publication date: July 28, 2011Inventors: Yuanlong Pan, Steven Hannah, Rondo Middleton
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Publication number: 20110184375Abstract: The present invention relates to new marker sequences for neurodegenerative diseases and the diagnostic use thereof together with a method for screening of potential active substances for neurodegenerative diseases by means of these marker sequences. Furthermore, the invention relates to a diagnostic device containing such marker sequences for neurodegenerative diseases, in particular a protein biochip and the use thereof.Type: ApplicationFiled: December 22, 2008Publication date: July 28, 2011Applicant: Protagen AktiengesellschaftInventors: Heike Göhler, Helmut E. Meyer, Katrin Marcus, Axel Kowald, Florian Tribl, Manfred Gerlach, Peter Riederer, Angelika Lüking, Christian Scheer, Jens Beator
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Publication number: 20110177976Abstract: Methods of modulating body fat or weight loss are presented Nucleic acid and protein microarrays that comprise biomolecules associated with an obese host microbiome or a lean host microbiome are utilized for analysis.Type: ApplicationFiled: June 30, 2009Publication date: July 21, 2011Applicant: THE WASHINGTON UNIVERSITYInventors: Jeffrey I. Gordon, Peter Turnbaugh
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Publication number: 20110172123Abstract: Protein arrays that allow the analysis of differentially expressed proteins in parallel are disclosed. Methods for making such arrays, and their use for screening and/or evaluating the effect of chemical entities, including drug and therapeutic moieties, are described. The arrays can also be used to screen for compounds, peptides, or proteins, which modulate the interaction between a protein and the differentially expressed protein.Type: ApplicationFiled: May 20, 2010Publication date: July 14, 2011Inventors: Roland Zbignieiw Kozlowski, Jonathan Michael Blackburn, Rhian Elizabeth Hayward, Grant William Wright Cameron
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Publication number: 20110172112Abstract: The present invention relates to a new combination of marker genes for characterizing a Lactobacillus sakei strain. In particular, the present invention concerns the use of a pattern of presence or absence of marker genes in the genome of the strain to be characterized for classifying and identifying said strain.Type: ApplicationFiled: February 20, 2009Publication date: July 14, 2011Inventors: Stéphane Chaillou, Monique Zagorec, Marie-Christine Champomier-Verges
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Publication number: 20110172125Abstract: Provided are compositions and methods for preparing and identifying antibodies having CDR3s that vary in sequence and in length from very short to very long which in certain embodiments may bind to a carbohydrate moiety or the active site of an enzyme. Libraries coding for antibodies with the CDR3s are also provided. The libraries can be provided by modifying a pre-existing nucleic acid library.Type: ApplicationFiled: April 24, 2009Publication date: July 14, 2011Applicant: DYAX CORP.Inventor: Robert C. Ladner
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Publication number: 20110172122Abstract: The present invention relates to methods for monitoring differential expression of a plurality of genes in a first filamentous fungal cell relative to expression of the same genes in one or more second filamentous fungal cells using microarrays containing Trichoderma reesei ESTs or SSH clones, or a combination thereof. The present invention also relates to computer readable media and substrates containing such array features for monitoring expression of a plurality of genes in filamentous fungal cells.Type: ApplicationFiled: June 5, 2008Publication date: July 14, 2011Applicant: Novozymes, Inc.Inventors: Randy Berka, Elena Bashkirova, Michael Rey
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Publication number: 20110172124Abstract: The invention provides a novel additive for improved analysis by mass spectrometry. More specifically, ascorbic acid has been found to reduce or eliminate the presence of adducts commonly present in mass spectra. The improved processes and compositions of the invention allow for increased accuracy, sensitivity and throughput for samples analyzed by mass spectrometry.Type: ApplicationFiled: January 7, 2011Publication date: July 14, 2011Applicant: SEQUENOM, INC.Inventor: Thomas Becker
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Publication number: 20110166868Abstract: Subscription-based systems and methods where a provider provides one or more customers, identified as subscribers or non-subscribers, with research products and services (e.g., for industries involved in genomic and proteomic research). Initially, the provider prepares collections of clones and provides customers with access to clone collections. Individual clones in a clone collection may comprise an ORF that may be flanked by recombination sites. Further, an ORF may contain a suppressible stop codon that may be suppressed to produce a fusion protein comprising the ORF and a tag sequence. Provider may provide additional related services and/or products. The products and services offered to the customers will vary depending on their designation as either subscribers or non-subscribers.Type: ApplicationFiled: March 1, 2010Publication date: July 7, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Lincoln Muir, August Sick, Nancy Groot, Dwayne W. Dexter, Charles Robinson, John Carrino, Robert Bennett
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Publication number: 20110160094Abstract: A method of stamping of molecular patterns and/or devices based on the reversible self-assembly of molecules, particularly organic molecules is disclosed. This method is suitable for the stamping of almost any nanofabricated device and can be used to transferring a large amount of pattern information from one substrate to another at the same time.Type: ApplicationFiled: January 4, 2011Publication date: June 30, 2011Inventors: Francesco STELLACCI, Arum Amy Yu
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Publication number: 20110160072Abstract: The present invention relates generally to a nucleic acid molecule, the RNA and protein expression profiles of which are indicative of the onset, predisposition to the onset and/or progression of a large intestine neoplasm. More particularly, the present invention is directed to a nucleic acid molecule, the expression profiles of which are indicative of the onset and/or progression of a colorectal neoplasm, such as an adenoma or an adenocarcinoma. The expression profiles of the present invention are useful in a range of applications including, but not limited to, those relating to the diagnosis and/or monitoring of colorectal neoplasms, such as colorectal adenomas and adenocarcinomas. Accordingly, in a related aspect the present invention is directed to a method of screening a subject for the onset, predisposition to the onset and/or progression of a large intestine neoplasm by screening for modulation in the expression profile of said nucleic acid molecule markers.Type: ApplicationFiled: October 23, 2008Publication date: June 30, 2011Applicants: CLINICAL GENOMICS PTY. LTD., COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATIONInventors: Lawrence Charles Lapointe, Robert Dunne, Graéme P. Young, Peter Molloy, Susanne Pedersen, Glenn Southwell Brown, Lloyd Douglas Graham
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Publication number: 20110158997Abstract: The invention is generally directed to antibody variable domains or antibodies, libraries of antibody variable domains or antibodies, methods of making said antibodies and libraries, and methods of treatment comprising administering the generated antibody variable domains or antibodies. Specifically, the invention is directed to novel primer nucleotide sequences that are used to amplify all rearranged sequences of canine variable heavy (VH) and variable light (VL) immunoglobulin chains that have been used in naturally occurring antibody responses. These novel sequences contain canine framework regions and complementarity determining regions which may be used to canine-ize antibodies. Further, these sequences are useful for the identification and targeting of viral and bacterial pathogens, and tumor-associated antigens.Type: ApplicationFiled: April 8, 2009Publication date: June 30, 2011Inventor: Nicola Mason
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Publication number: 20110152126Abstract: A method for generating a variant library of DNA sequences starting from at least one DNA starting sequence and including the following steps: a) selecting at least two mutation sites in the starting sequence; b) dividing the DNA starting sequence into at least two sequence segments in such a way that at least two of these sequence segments each contain at least one of the mutation sites; c) amplifying the sequence segments by polymerase chain reaction with the aid of a total of at least five different oligonucleotides, where at (i) least one of the oligonucleotides can attach to each of the mutation sites; (ii) at least two of the oligonucleotides can attach to at least one mutation site, and (iii) mutations are introduced, via mismatch positions, into the PCR amplificates by the oligonucleotides at the mutation sites where at least two mutations are introduced at least one of the mutation sites; and d) linking the amplificates to give DNA sequences.Type: ApplicationFiled: December 3, 2010Publication date: June 23, 2011Applicant: c-LEcta GmbHInventors: Thomas GREINER-STOEFFELE, Claudi FELLER, Marc STRUHALLA
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Publication number: 20110152109Abstract: Biological sample target classification, detection and selection methods are described, together with related arrays and oligonucleotide probes.Type: ApplicationFiled: December 21, 2009Publication date: June 23, 2011Inventors: Shea N. Gardner, Crystal J. Jaing, Kevin S. McLoughlin, Thomas R. Slezak
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Publication number: 20110143418Abstract: Orthogonal ribosome orthogonal mRNA pairs are provided, as are methods for their selection involving a novel positive-negative selection approach, and methods for their use. Also provided are cellular logic circuits involving orthogonal ribosomes.Type: ApplicationFiled: December 8, 2010Publication date: June 16, 2011Inventors: OLIVER RACKHAM, Jason W. Chin
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Publication number: 20110143956Abstract: Variations in certain genomic sequences useful as genetic markers of Sudden Cardiac Death (“SCD”) or Sudden Cardiac Arrest (“SCA”) risk are described. Novel diagnostic kits, DNA microarrays, and methods employing these genetic markers are used in assessing the risk of SCD or SCA. Methods of distinguishing patients having an increased susceptibility to SCD or SCA, through use of these markers, alone or in combination with other markers, are also provided. Further, methods of detecting a polymorphism associated with SCD or SCA are taught.Type: ApplicationFiled: December 7, 2010Publication date: June 16, 2011Applicant: MEDTRONIC, INC.Inventors: Orhan Soykan, Tara Nahey, Jeffrey Lande
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Publication number: 20110136676Abstract: The invention is related to nucleic acid arrays and methods of using nucleic acid arrays.Type: ApplicationFiled: October 25, 2010Publication date: June 9, 2011Inventor: Eric C. Greene
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METHOD FOR DETERMINING A PREDISPOSITION TO BASAL CELL CARCINOMA AND FOR SCREENING TREATMENTS THEREOF
Publication number: 20110136685Abstract: The present invention relates to methods for detecting a predisposition to a basal cell carcinoma and methods for screening a treatment of a basal cell carcinoma.Type: ApplicationFiled: August 4, 2009Publication date: June 9, 2011Applicants: INSTITUT GUSTAVE ROUSSY, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, UNIVERSITE PARIS DIDEROT - PARIS 7, UNIVERSITE PARIS-SUD XIInventors: Alexandre Valin, Thierry Magnaldo -
Publication number: 20110130308Abstract: A multiplexed array and method for fabricating a multiplexed array are disclosed. The multiplexed array includes a hydrophobic barrier formed on a substrate. The hydrophobic barrier includes a plurality of wells in which microarrays are located. A liquid cover slip is positioned to seal each of the wells.Type: ApplicationFiled: December 1, 2010Publication date: June 2, 2011Inventors: John A. Luckey, Emile Nuwaysir
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Patent number: 7951753Abstract: Libraries of nucleic acids encoding chimeric binding polypeptides based on plant scaffold polypeptide sequences. Also described are methods for generating the libraries.Type: GrantFiled: February 13, 2007Date of Patent: May 31, 2011Assignee: Divergence, Inc.Inventor: Jennifer Jones
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Publication number: 20110118127Abstract: The present invention relates to a marker gene for screening a drug inducing toxicity in human and a screening method using the same. More precisely, the invention relates to a microarray on which marker genes up-or down-regulated specifically by 16 drugs inducing pulmonary toxicity, teratogenicity, nephrotoxicity, cardiotoxicity or mutation (Methotrexate, Nitrofurantoin, Amiodarone, Carbamazepine, Valproic acid, Thalidomide, Cisplatin, Gentamycin, Amphotericine, Furylfuramide, N-nitroso-N-methylurea, methylmethanesulfonate, 4-nitroquinoline-N-oxide, 2-nitrofluorene, Doxorubicin and Daunorubicin) are integrated, a kit comprising the said microarray, and a screening method of a drug inducing toxicity in human using the same.Type: ApplicationFiled: March 9, 2010Publication date: May 19, 2011Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Jae-Chun RYU, Youn Jung KIM, Mee SONG, Mi-Kyung SONG, Han Seam CHOI, Ji Hee KIM, Jina LEE
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Publication number: 20110112175Abstract: The invention provides an expression cassette comprising a DNA sequence encoding amino acids 1-99 of human preproenkephalin, a DNA sequence encoding a precursor of a carboxy-amidated peptide flanked by dibasic cleavage sites and optionally a DNA sequence encoding a marker protein (such as Enhanced Green Fluorescent Protein (GNP)) all in operable linkage and under control of a promoter. Where the encoded precursor of a carboxy-amidated peptide is an agonist for an opioid receptor, the invention further provides a method of treating neuropathic pain by administering the gene transfer vector comprising such an expression cassette to a patient.Type: ApplicationFiled: September 29, 2010Publication date: May 12, 2011Applicants: REGENTS OF THE UNIVERSITY OF MICHIGANInventors: DARREN P. WOLFE, JOSEPH C. GLORIOSO, DAVID J. FINK
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Publication number: 20110113498Abstract: Provided herein are Sirt1 polymorphic variants having a substitution at amino acid residue 107 or nucleotide 373. In certain embodiments, the Sirt1 polypeptide variants have a L107P substitution and the nucleic acid variants have a T373C substitution. Genetic and/or biochemical testing may be performed to identify whether a patient carries one of the disclosed polymorphic variants. Based on the polymorphic variant the patient carries, a medical practitioner may administer an appropriate therapy, such as a sirtuin activator.Type: ApplicationFiled: May 15, 2009Publication date: May 12, 2011Inventors: Christoph H. Westphal, Marc Donath, Peter Elliott, Michael Jirousek, Jill Milne
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Publication number: 20110105342Abstract: The invention provide methods for early detection of a reduced risk of developing cancer, which comprises detecting the absence of a series of genetic polymorphisms associated with a predisposition of developing cancer, including the polymorphisms of the genes BRCA1, BRCA2, CARD15 (NOD2), CHEK2, CDKN2A (P16), CYP1B1, FGFR2 (KGFR2), MAP3K1 (MEKK1), p53 (TP53), TNRC9, XPD (ERCC2) and the genetic marker Rs6983267, in a biological sample from the analyzed subject, wherein the absence of the genetic polymorphisms is indicative of significantly decreased risk of developing, at least, breast cancer.Type: ApplicationFiled: March 13, 2009Publication date: May 5, 2011Inventors: Jan Lubinski, Tadeusz Debniak, Grzegorz Kurzawski, Anna Jakubowska, Krzystof Medrek, Cezary Cybulski, Jacek Gronwald, Tomasz Huzarski, Bohdan Gorski, Tomasz Byrski, Joanna Matyjasik, Pablo Serrano Fernandez
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Publication number: 20110098189Abstract: The present invention relates generally to nucleic acid molecules in respect of which changes to the DNA or to the RNA or protein expression profiles are indicative of the onset, predisposition to the onset and/or progression of a neoplasm. More particularly, the present invention is directed to nucleic acid molecules in respect of which changes to the DNA or to the RNA or protein expression profiles are indicative of the onset and/or progression of a large intestine neoplasm, such as an adenoma or an adenocarcinoma. The DNA or the expression profiles of the present invention are useful in a range of applications including, but not limited to, those relating to the diagnosis and/or monitoring of colorectal neoplasms, such as colorectal adenocarcinomas.Type: ApplicationFiled: October 23, 2008Publication date: April 28, 2011Applicants: CLINICAL GENOMICS PTY. LTD.Inventors: Lawrence C. Lapointe, Robert Dunne, Graeme P. Young, Trevor John Lockett, William J. Wilson, Peter Laurence Molloy
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METHODS FOR PRODUCING SUBSTANTIALLY HOMOGENEOUS HYBRID OR COMPLEX N-GLYCANS IN METHYLOTROPHIC YEASTS
Publication number: 20110092374Abstract: The present invention provides methods for effectively and efficiently converting methylotrophic yeast's heterogeneous high mannose-type N-glycosylation to mammalian-type N-glycosylation by disruption of an endogenous glycosyltransferase gene (OCH1) and step-wise introduction of heterologous glycosidase and glycosyltransferase activities. Each engineering step includes a number of stages: transformation with an appropriate vector, cultivation of a number of transformants, performance of sugar analysis and heterologous protein expression analysis, and selection of a desirable clone. The selected clone is then subjected to the next engineering step.Type: ApplicationFiled: October 18, 2010Publication date: April 21, 2011Applicants: VIB, VZW, UNIVERSITEIT GENT, RESEARCH CORPORATION TECHNOLOGIES, INC.Inventors: Nico Callewaert, David A. Wiersma -
ASSESSMENT OF EFFECT OF AN AGENT ON A HUMAN BIOLOGICAL CONDITION USING RODENT GENE EXPRESSION PANELS
Publication number: 20110092390Abstract: Rodent gene expression data, in particular, gene expression profiles, are created and used to predict the efficacy of therapeutic agents on human biological conditions. Gene Profile data sets are derived from rodent subject samples and include quantitative, substantially repeatable measures of a distinct amount of RNA or protein constituent(s) in a signature panel selected such that measurement of the constituent(s) enables measurement of a biological condition of interest in both human and rodent subjects. Such profile data sets may be used to predict the therapeutic efficacy of a therapeutic agent in humans.Type: ApplicationFiled: July 29, 2010Publication date: April 21, 2011Applicant: Source Precision Medicine, Inc.Inventors: Michael Bevilacqua, Victor Tryon, John Cheronis, Danute Bankaitis-Davis, Kathleen Storm, Karl Wassmann -
Publication number: 20110086772Abstract: Multiplex (+/?) stranded analyses, such as array comparative genomic hybridization (aCGH), are provided for detecting chromosomal rearrangements associated with cancer and other diseases. For example, an illustrative multiplex array for CGH includes discrete plus (+) strand and minus (?) strand DNA probes, complementary to each other but separable on the CGH array. The minus (?) strand DNA probes recover diagnostic information lost to conventional microarrays, since many genes transcribe from the minus (?) strand. In an illustrative system, patient and control DNA samples are prepared for CGH by amplification and labeling using comprehensive primers that generate both plus (+) strands and minus (?) strands of DNA in the samples. The breakpoints of a translocated chromosome may be detected on a multiplex microarray by DNA probes of one polarity, while DNA copy number changes associated with the translocation region may be detected by corresponding DNA probes of the complementary polarity.Type: ApplicationFiled: September 27, 2010Publication date: April 14, 2011Applicant: Signature Genomics Laboratories LLCInventors: Lisa McDaniel, Blake Ballif, Roger Schultz, Brice Tebbs, Bassem Bejjani, Lisa Shaffer