Encodes An Enzyme Patents (Class 536/23.2)
-
Patent number: 9029112Abstract: The present disclosure relates to engineered ketoreductase polypeptides and uses thereof for the preparation of ?-chloroalcohols from ?-chloroketones. Also provided are polynucleotides encoding the engineered ketoreductase polypeptides and host cells capable of expressing the engineered ketoreductase polypeptides.Type: GrantFiled: June 24, 2014Date of Patent: May 12, 2015Assignee: Codexis, Inc.Inventors: Yong Koy Bong, Michael Vogel, Steven J. Collier, Vesna Mitchell, Jagadeesh Mavinahalli
-
Patent number: 9029115Abstract: The pharmaceutical use of lipases related to the Thermomyces lanuginosus (Humicola lanuginosa) lipase comprising amino acids 1-269 of SEQ ID NO: 2, optionally in combination with a protease and/or an amylase. Examples of medical indications are: Treatment of digestive disorders, pancreatic exocrine insufficiency (PEI), pancreatitis, cystic fibrosis, diabetes type I, and/or diabetes type II. The lipases of the invention have, e.g., an improved digestion performance in vitro, an improved activity at a pH in the neutral range, an improved stability at low pH, and are stable against protease-degradation, and/or are stable in the presence of pepsin and bile salts. The invention also relates to methods of determining digestion performance in vitro of lipases, as well as to certain novel variants of the lipase of T. lanuginosus.Type: GrantFiled: August 16, 2012Date of Patent: May 12, 2015Assignees: Novozymes A/S, Novozymes IncInventors: Allan Svendsen, Michael Skjoet, Deborah Yaver, Lars Lehmann Hylling Christensen, Signe Eskildsen Larsen, Nina Lundin, Michael Lamsa, Peter Colin Gregory
-
Patent number: 9028813Abstract: The present invention relates to a designer or recombinant ubiquitin ligase molecule that includes a toxin binding domain that is specific for a toxin active fragment, wherein the toxin active fragment is an enzymatically active fragment of one or more toxins or toxin serotypes; and an E3-ligase domain that comprises an E3-ligase or polypeptide that facilitates E2-mediated ubiquitination of the toxin active fragment. In an embodiment, the composition further includes a delivery system that allow the designer ubiquitin ligase to enter the cell. The present invention further includes methods for treating an individual intoxicated with a toxin by administering the designer ubiquitin ligase of the present invention.Type: GrantFiled: December 26, 2012Date of Patent: May 12, 2015Assignee: Synaptic Research, LLCInventors: George A. Oyler, Yien Che Tsai
-
Patent number: 9029114Abstract: Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods for modulating or altering recombination inside or outside of a cell using isolated and/or purified polypeptides and/or nucleic acid sequences from Alicyclobacillus acidocaldarius.Type: GrantFiled: October 17, 2013Date of Patent: May 12, 2015Assignee: Battelle Energy Alliance, LLCInventors: Brady D. Lee, Deborah T. Newby, Jeffrey A. Lacey, David N. Thompson, Vicki S. Thompson, William A. Apel, Francisco F. Roberto, David W. Reed
-
Patent number: 9029087Abstract: Compositions, methods and related uses are provided relating to cleaving modified DNA. For example, a set of DNA fragments obtainable by enzymatic cleavage of a large DNA is described where at least 50% are similarly sized and have a centrally positioned modified nucleotide. In addition, an enzyme preparation is provided that includes one or more enzymes that recognize a modified nucleotide in a DNA and cleave the DNA at a site that is at a non-random distance from the modified nucleotide. The one or more enzymes are further characterized by an N-terminal conserved domain with greater than 90% amino acid sequence homology to WXD(X)10YXGD. The related uses include creating a methylome, methods of purifying DNA fragments containing a modified nucleotide and diagnostic applications.Type: GrantFiled: August 16, 2013Date of Patent: May 12, 2015Assignee: New England Biolabs, Inc.Inventors: Yu Zheng, Richard J. Roberts
-
Publication number: 20150125903Abstract: The present invention provides fungal xylanase and/or xylosidase enzymes suitable for use in saccharification reactions. The present invention provides xylanase and xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase(s) and/or xylosidase(s), as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures. In some embodiments, the xylanase and xylosidase enzyme(s) are M. thermophila enzymes.Type: ApplicationFiled: January 14, 2015Publication date: May 7, 2015Inventors: Nicholas John Agard, David Elgart, Jie Yang, Goutami Banerjee, Jeanne Bonomo Benoit, Dipnath Baidyaroy
-
Publication number: 20150125936Abstract: Provided herein are isolated laccase enzymes and nucleic acids encoding them. Also provided are mediators for laccase reactions. Also provided herein are methods for using laccases to oxidize lignins and other phenolic and aromatic compounds, such as for bio-bleaching and decolorization of wood pulp under high temperature and pH conditions to facilitate a substantial reduction in use of bleaching chemicals, as well as for treatment of fibers.Type: ApplicationFiled: January 7, 2015Publication date: May 7, 2015Inventors: JANNE SAMULI KEROVUO, SYLKE HAREMZA, OLIVER KOCH, TILO HABICHER, DAN E. ROBERTSON, GRACE DESANTIS, RYAN MCCANN, PETER LUGINBUHL
-
Publication number: 20150125438Abstract: The present invention relates to a peptide with anti-inflammatory activity, wherein the peptide comprises any one amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 161, the peptide has above 80% homology of amino acid sequence with above-mentioned sequences, or the peptide is the fragment of the above-mentioned peptides. The present invention also relates to an inflammatory composition comprising the above mentioned peptides. According to the present invention, a peptide that has at least one amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 161 has outstanding efficacy in both suppressing inflammation and in prophylactic means. Therefore, the composition comprising the peptides of this invention can be used as anti-inflammatory pharmaceutical compositions or as cosmetic compositions, in turn, treating and preventing a variety of different types of inflammatory diseases.Type: ApplicationFiled: March 5, 2013Publication date: May 7, 2015Inventors: Sang Jae Kim, Kyung Hee Kim, Kyu-Yong Lee, Seong-Ho Koh, Bum Joon Kim, Hyun-Hee Park, Sung Jin Huh, Woo Jin Lee, Hwain Jang, Jung Soon Ha
-
Publication number: 20150125924Abstract: A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.Type: ApplicationFiled: June 2, 2014Publication date: May 7, 2015Inventors: Aldis Darzins, Lance P. Encell, Dieter Klaubert, Georgyi V. Los, Mark McDougall, Keith V. Wood, Monika G. Wood, Chad Zimprich
-
Publication number: 20150128306Abstract: The invention provides a method for producing steviol glycosides. The invention provides a transformant having introduced therein the steviol glucosyltransferase and a method for producing steviol glycosides using the transformant.Type: ApplicationFiled: March 14, 2013Publication date: May 7, 2015Applicant: SUNTORY HOLDINGS LIMITEDInventor: Eiichiro Ono
-
Publication number: 20150128301Abstract: Nucleic acid molecules from cannabis has been isolated and characterized and encode polypeptides having aromatic prenyltransferase activity. Expression or over-expression of the nucleic acids alters levels of cannabinoid compounds. The polypeptides may be used in vivo or in vitro to produce cannabinoid compounds.Type: ApplicationFiled: October 9, 2014Publication date: May 7, 2015Inventors: Jonathan E. Page, Zakia Boubakir
-
Publication number: 20150125902Abstract: Provided are isolated polypeptides having glucoamylase activity, catalytic domains, and polynucleotides encoding the polypeptides, catalytic domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains.Type: ApplicationFiled: August 24, 2012Publication date: May 7, 2015Applicant: Novozymes A/SInventors: Tianqi Sun, Ming Li
-
Patent number: 9023637Abstract: Provided herein are fungi, such as Aspergillus niger, having a dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase (Alg3) gene genetic inactivation, increased expression of a loss of aflR expression A (Lae), or both. In some examples, such mutants have several phenotypes, including an increased production of citric acid relative to the parental strain. Methods of using the disclosed fungi to make citric acid are also provided, as are compositions and kits including the disclosed fungi.Type: GrantFiled: November 30, 2012Date of Patent: May 5, 2015Assignee: Battelle Memorial InstituteInventors: Ziyu Dai, Scott E. Baker
-
Patent number: 9024003Abstract: The present invention notably relates to novel recombinant telomerase reverse transcriptases, nucleic acid molecules coding them, cells comprising said nucleic acid molecule and use of these cells for the production of substance of interest.Type: GrantFiled: January 5, 2007Date of Patent: May 5, 2015Assignee: Transgene S.A.Inventors: Philippe Erbs, Jean-Marc Balloul, Marina Kapfer, Nathalie Silvestre
-
Patent number: 9023627Abstract: Systems, compounds and methods for the conversion of C1 carbon compounds to higher carbon compounds useful for the generation of commodity compounds.Type: GrantFiled: September 15, 2011Date of Patent: May 5, 2015Assignee: University of Washington through its Center for CommercializationInventors: Justin Siegel, David Baker, Amanda Lee Smith, Mary E. Lidstrom, Catherine Louw
-
Patent number: 9023629Abstract: The present invention relates to host cells transformed with a nucleic acid sequence encoding a eukaryotic xylose isomerase obtainable from an anaerobic fungus. When expressed, the sequence encoding the xylose isomerase confers to the host cell the ability to convert xylose to xylulose which may be further metabolized by the host cell. Thus, the host cell is capable of growth on xylose as carbon source. The host cell preferably is a eukaryotic microorganism such as a yeast or a filamentous fungus. The invention further relates to processes for the production of fermentation products such as ethanol, in which a host cell of the invention uses xylose for growth and for the production of the fermentation product. The invention further relates to nucleic acid sequences encoding eukaryotic xylose isomerases and xylulose kinases as obtainable from anaerobic fungi.Type: GrantFiled: November 29, 2012Date of Patent: May 5, 2015Assignee: DSM IP Assets B.V.Inventors: Hubertus Johannes Marie Op Den Camp, Harry Ramanoedj Harhangi, Christiaan Van Der Drift, Jacobus Thomas Pronk
-
Patent number: 9023608Abstract: The present invention relates to glucose dehydrogenase [NAD(P)+GDH] using nicotinamide adenine dinucleotide or nicotinamide adenine dinucleotide phosphate as a coenzyme, in which the thermal stability and/or the resistance to an organic solvent in the absence of sodium chloride are improved.Type: GrantFiled: March 30, 2012Date of Patent: May 5, 2015Assignee: Nipro CorporationInventors: Masafumi Yao, Shido Kawase, Shin-ichi Yokobori, Akihiko Yamagishi
-
Patent number: 9024114Abstract: Compositions and methods related to transgenic AHAS-inhibiting herbicide resistant soybean plants are provided. Event 127 soybean plants having a mutated AHAS coding sequence which imparts tolerance to an AHAS-inhibiting herbicide are provided. The event 127 soybean plants having the event 127 nucleic acid molecule at the identified chromosomal location may comprise genomic/transgene junctions having at least the nucleic acid sequence of SEQ ID NO: 5 and/or 6. The characterization of the genomic insertion site of the event 127 provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the event 127 soybean plants are provided.Type: GrantFiled: January 6, 2010Date of Patent: May 5, 2015Assignees: Empresa Brasileira de Pesquisa Agropecuaria—Embrapa, BASF Agrochemical Products B.V.Inventors: Dale Carlson, Francisco Jose Lima Aragao, Carlos Alberto Arrabal Arias, Luiz Louzano, Bruce M. Luzzi, Tim Malefyt, Elibio Leopoldo Rech Filho, Siyuan Tan, Adolfo Ulbrich, Tadashi Yotsumoto, Ute Linemann
-
Patent number: 9023636Abstract: The invention provides non-naturally occurring microbial organisms having a propylene pathway. The invention additionally provides methods of using such organisms to produce propylene.Type: GrantFiled: April 27, 2011Date of Patent: May 5, 2015Assignee: Genomatica, Inc.Inventors: Mark J. Burk, Anthony P. Burgard
-
Patent number: 9023632Abstract: The present invention relates to bacterial strains, capable of utilizing glycerol as a carbon source for the fermentative production of succinic acid, wherein said strains are genetically modified so that they comprise a deregulation of their endogenous pyruvate-formate-lyase enzyme activity, as well as to methods of producing organic acids, in particular succinic acid, by making use of such microorganism. The present invention also relates to the downstream processing of the produced organic acids by cation exchange chromatography.Type: GrantFiled: January 8, 2014Date of Patent: May 5, 2015Assignee: BASF SEInventors: Hartwig Schröder, Stefan Haefner, Gregory Von Abendroth, Rajan Hollmann, Aline Raddatz, Hansgeorg Ernst, Hans Gurski
-
Publication number: 20150118356Abstract: A variant polypeptide having chymosin activity, wherein the variant has an amino acid sequence which, when aligned with the chymosin comprising the sequence set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue corresponding to any of amino acids 2, 22, 40, 48, 50, 51, 53, 61, 62, 76, 88, 98, 99, 109, 112, 117, 125, 126, 135, 144, 160, 161, 163, 187, 189, 194, 200, 201, 202, 203, 221, 223, 240, 242, 244, 254, 267, 271, 273, 278, 280, 284, 289, 292, 294 or 295 said positions being defined with reference to SEQ ID NO: 2 and wherein the variant has one or more altered properties as compared with a reference polypeptide having chymosin activity. Such a variant polypeptide may be used in the preparation of a cheese.Type: ApplicationFiled: May 3, 2013Publication date: April 30, 2015Inventors: Petrus Jacobus Theodorus Dekker, René Marcel De Jong, Cornelis Marinus Muijlwijk
-
Publication number: 20150118719Abstract: The present disclosure provides engineered proline hydroxylase polypeptides for the production of hydroxylated compounds, polynucleotides encoding the engineered proline hydroxylases, host cells capable of expressing the engineered proline hydroxylases, and methods of using the engineered proline hydroxylases to prepare compounds useful in the production of active pharmaceutical agents. The present disclosure provides engineered proline hydroxylase biocatalysts, polynucleotides encoding the biocatalysts. methods of their preparation, and processes for preparing hydroxylated compounds using these engineered biocatalysts.Type: ApplicationFiled: May 7, 2013Publication date: April 30, 2015Inventors: Haibin Chen, Yong Koy Bong, Fabien L. Cabirol, Anupam Gohel Prafulchandra, Tao Li, Jeffrey C. Moore, Martina Quintanar-Audelo, Yang Hong, Steven J. Collier, Derek Smith
-
Publication number: 20150118700Abstract: This invention provides an amadoriase having high reactivity with ?-fructosyl hexapeptide (?F6P) derived from the ?-chain amino terminus of glycated hemoglobin (HbAlc), which enables satisfactory quantification of ?F6P cleaved from HbAlc. A novel amadoriase is derived from the amadoriase of the genus Coniochaeta by substitution of one or more amino acids at positions selected from the group consisting of positions 62, 63, 102, 106, 110, 113, 355, and 419. Such amadoriase enables rapid, simple, accurate, and satisfactory quantification of ?F6P cleaved from HbAlc. With the use of such amadoriase, a method for measurement of HbAlc by an enzymatic method and a kit for measurement of HbAlc that are based on the same principle as used in the conventional standard method and excellent in terms of the relevance with the standard method.Type: ApplicationFiled: April 26, 2013Publication date: April 30, 2015Inventors: Atsushi Ichiyanagi, Yosuke Masakari
-
Publication number: 20150118731Abstract: The application relates to antimicrobial agents against Gram-negative bacteria, in particular to fusion proteins composed of an enzyme having the activity of degrading the cell wall of Gram-negative bacteria and a peptide stretch fused to the enzyme at the N- or C-terminus, as well as pharmaceutical compositions comprising the same. Moreover, it relates to nucleic acid molecules encoding such a fusion protein, vectors comprising said nucleic acid molecules and host cells comprising either said nucleic acid molecules or said vectors. In addition, it relates to such a fusion protein for use as a medicament, in particular for the treatment or prevention of Gram-negative bacterial infections, as diagnostic means or as cosmetic substance. The application also relates to the treatment or prevention of Gram-negative bacterial contamination of foodstuff, of food processing equipment, of food processing plants, of surfaces coming into contact with foodstuff, of medical devices, of surfaces in hospitals and surgeries.Type: ApplicationFiled: November 7, 2014Publication date: April 30, 2015Applicants: LYSANDO AG, KATHOLIEKE UNIVERSITEIT LEUVEN, K.U. LEUVEN R&DInventors: Rob LAVIGNE, Stefan MILLER, Yves BRIERS, Guido VOLCKAERT, Maarten WALMAGH
-
Publication number: 20150118721Abstract: The invention relates to novel enzymes that provide acetylated sphingoid bases.Type: ApplicationFiled: March 13, 2013Publication date: April 30, 2015Applicant: EVONIK INDUSTRIES AGInventors: Steffen Schaffer, Mike Farwick, Heiko Andrea, Tim Koehler, Daniel Wolff, Frank ter Veld, Ansgar Poetsch, Eckhard Boles, Christoph Schorsch
-
Publication number: 20150118228Abstract: A chimeric ErbB ligand binding molecule is disclosed along with its pharmaceutically acceptable salt forms. The molecule is a protein that as part of its sequence includes the sequence of SEQ ID NOS: 1, 2, or 3. The molecule can be fused to an IgGFc and especially IgGFc containing cysteine to serine changes in the hinge region. For example, the fusion can be to IgG 1Fc DNA sequences that encode the binding molecules are also contemplated as well as vectors containing such DNA sequences and hosts that contain such vectors. Pharmaceutical compositions are contemplated that contain the binding molecule along with a pharmaceutically acceptable excipient.Type: ApplicationFiled: April 25, 2013Publication date: April 30, 2015Inventor: Jason E. Hill
-
Patent number: 9017982Abstract: Provided are non-wild-type organophosphorus acid anhydrolases that are capable of degrading (ethyl {2-[bis(propan-2-yl)amino]ethyl}sulfanyl) (methyl)phosphinate and other V-agents. Particular embodiments include an organophosphorus acid anhydrolase including an amino acid substitution at position 212.Type: GrantFiled: December 17, 2013Date of Patent: April 28, 2015Assignee: The United States of America as Represented by the Secretary of the ArmyInventors: Saumil S. Shah, Mark A. Guelta, Steven P. Harvey
-
Publication number: 20150111268Abstract: Recombinant butyraldehyde dehydrogenases (Blds) with improved production of 1,4-BDO, as well as recombinant microorganisms comprising polynucleotides encoding the recombinant Blds, and methods of producing 1,4-BDO by using the recombinant microorganisms.Type: ApplicationFiled: October 1, 2014Publication date: April 23, 2015Inventors: Jinhwan Park, Pyungcheon Lee, Jaechan Park, Youngmin Lee, Wooyong Lee, Jinwoo Park, Kwangmyung Cho, Heejin Hwang
-
Publication number: 20150110765Abstract: The present invention relates to isolated polypeptides having alpha-glucuronidase activity, catalytic domains and polynucleotides encoding the polypeptides, catalytic domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides or catalytic domains.Type: ApplicationFiled: April 22, 2013Publication date: April 23, 2015Inventors: Johan Börjesson, Anders Vikø-Nielsen, Nikolaj Spodsberg, Kristian Krogh
-
Publication number: 20150111278Abstract: The present invention relates to use of Caminibacter carbonic anhydrase in CO2 extraction, e.g., from flue gas, natural gas, biogas or ambient air. The Caminibacter carbonic anhydrases are especially well suited for these purpose due to their extreme thermostability.Type: ApplicationFiled: December 17, 2014Publication date: April 23, 2015Inventors: Martin Borchert, Paria Saunders
-
Publication number: 20150111269Abstract: Methods for the evolution of NADPH specific ketol-acid reductoisomerase enzymes to acquire NADH specificity are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to utilize NADH are described.Type: ApplicationFiled: December 23, 2014Publication date: April 23, 2015Inventors: Yougen LI, Der-Ing LIAO, Mark J. NELSON, Daniel P. OKEEFE
-
Publication number: 20150110830Abstract: Detoxified variants of the pathogenic E. coli ‘AcfD precursor’ (orf3526) have been identified that raise a substantially similar immune response in a subject as the native AcfD (orB526) protein. The detoxified variants may be further modified to have increased solubility as compared to the native AcfD (orf3526) protein.Type: ApplicationFiled: October 27, 2014Publication date: April 23, 2015Inventors: Laura SERINO, Maria Rita FONTANA, Danilo GOMES MORIEL
-
Patent number: 9012617Abstract: Dual colored fluorescent indicators of specific tyrosine kinase activity and methods of use thereof are disclosed.Type: GrantFiled: April 11, 2012Date of Patent: April 21, 2015Assignee: The Trustees of The University of PennsylvaniaInventors: Matthew Dalva, Julia X. Zhu
-
Patent number: 9012196Abstract: A substrate or coating is provided that includes a lipase with enzymatic activity toward a component of a fingerprint. Also provided is a process for facilitating the removal of fingerprints is provided wherein an inventive substrate or coating including a lipase is capable of enzymatically degrading of one or more components of the fingerprint to facilitate fingerprint removal from the substrate or said coating. Applying heat to the substrate or coating increases the rate of fingerprint removal.Type: GrantFiled: February 4, 2013Date of Patent: April 21, 2015Assignees: Toyota Motor Engineering & Manufacturing North America, Inc., Toyota Motor Corporation, Regents of the University of MinnesotaInventors: Andreas Buthe, Ping Wang, Songtao Wu, Hongfei Jia, Masahiko Ishii, Minjuan Zhang
-
Patent number: 9012186Abstract: The present disclosure provides methods for the conversion of hemicellulose into fermentable sugars using enzymes isolated from Prevotella bryantii. Hemicellulose-degrading enzymes include an endoxylanase, a ?-xylosidase, a bifunctional ?-xylosidase and ?-glucosidase, a bifunctional arabinofuranosidase and ?-xylosidase, a glucuronidase, and an acetyl xylan esterase. The enzymes can be used to release sugars present in hemicellulose for subsequent fermentation to produce value-added products such as ethanol.Type: GrantFiled: April 27, 2010Date of Patent: April 21, 2015Assignee: The Board of Trustees of the University of IllinoisInventors: Isaac K. O. Cann, Shinichi Kiyonari, Dylan Dodd, Yejun Han, Roderick I. Mackie
-
Patent number: 9012190Abstract: The invention relates generally to the field of industrial microbiology and alcohol production. More specifically, the invention relates to the use of thiamine, biosynthetic precursors of thiamine, nicotinic acid, nicotinamid, nicotinic acid riboside, nicotinamid riboside, or other biosynthetic precursors of nicotine adenine dinucleotide (NAD) to improve butanol production. Butanol production can be improved by providing sufficient amounts of thiamine, biosynthetic precursors of thiamine, nicotinic acid, nicotinamid, nicotinic acid riboside, nicotinamid riboside, or other biosynthetic precursors of nicotine adenine dinucleotide (NAD) in the production media.Type: GrantFiled: June 15, 2011Date of Patent: April 21, 2015Assignee: Butamax Advanced Biofuels LLCInventors: Michael Dauner, Mehmedalija Jahic
-
Patent number: 9012199Abstract: Recombinant carrier molecules having amino acid sequences from thermostable enzymes and methods of use for expression, recovery and delivery of foreign sequences (peptides and polypeptides) produced in different systems (bacteria, yeast, DNA, cell cultures such as mammalian, plant, insect cell cultures, protoplast and whole plants in vitro or in vivo are provided. The recombinant carrier molecule using sequences from lichenase B (Lic B) were also made and used as part of carrier protein to express, recover and deliver a variety of target polypeptides of interest.Type: GrantFiled: October 23, 2013Date of Patent: April 21, 2015Assignee: iBio, Inc.Inventors: Vidadi Yusibov, Vadim Mett, Konstantin Musiychuk
-
Patent number: 9012195Abstract: The present invention is directed to non-cytotoxic protein conjugates for inhibition or reduction of exocytic fusion in a nociceptive sensory afferent cell. The protein conjugates comprise: (i) a dynorphin Targeting Moiety (TM), wherein the TM is an agonist of a receptor present on a nociceptive sensory afferent cell, and wherein the receptor undergoes endocytosis to be incorporated into an endosome within the nociceptive sensory afferent cell; (ii) a non-cytotoxic protease or a fragment thereof, wherein the protease or protease fragment is capable of cleaving a protein of the exocytic fusion apparatus of the nociceptive sensory afferent cell; and (iii) a Translocation Domain, wherein the Translocation Domain translocates the protease or protease fragment from within the endosome, across the endosomal membrane, and into the cytosol of the nociceptive sensory afferent cell. Nucleic acid sequences encoding the protein conjugates, methods of preparing same and uses thereof are also described.Type: GrantFiled: July 17, 2013Date of Patent: April 21, 2015Assignees: Syntaxin, Ltd., Allergan, Inc.Inventors: Keith Foster, John Chaddock, Charles Penn, Kei Roger Aoki, Joseph Francis, Lance Steward
-
Patent number: 9012197Abstract: The present invention is directed to recombinant microalgal cells and their use in production of heterologous hemagglutinin-neuraminidase (HN) polypeptides, as well as compositions and uses thereof.Type: GrantFiled: December 28, 2010Date of Patent: April 21, 2015Assignees: Merial, Inc., Sanofi Vaccine Technologies, S.A.S.Inventors: Anne-Cécile V. Bayne, James Casey Lippmeier, Kirk Emil Apt, Xuan Guo, Joyce A. Pritchard
-
Publication number: 20150106971Abstract: There is disclosed a mutant, non-naturally occurring or transgenic plant cell comprising: (i) a polynucleotide comprising, consisting or consisting essentially of a sequence encoding a threonine synthase and having at least 90% sequence identity to SEQ ID NO:1, SEQ ID NO:2 or SEQ ID NO:3 or at least 87% sequence identity to SEQ ID NO:4, or SEQ ID NO:5; (ii) a polypeptide encoded by any one of said polynucleotides set forth in (i); or (iii) a polypeptide having at least 95% sequence identity to SEQ ID NO:6, SEQ ID NO:7 or SEQ ID NO:8; or (iv) a construct, vector or expression vector comprising the polynucleotide as set forth in (i).Type: ApplicationFiled: August 31, 2012Publication date: April 16, 2015Applicant: PHILIP MORRIS PRODUCTS S.A.Inventors: Lucien Bovet, Nicolas Sierro
-
Publication number: 20150106963Abstract: Novel mutations in cytochrome P450C17 (CYP17) and cytochrome b5 (CYB5) affecting 16-androstene steroid synthesis are disclosed. The novel mutations result in alterations in production of critical intermediaries in the synthesis of 16-androstene steroids. Altering the activity of these enzymes may be useful in enhancing reducing androstenone synthesis and reducing boar taint. The identification of these novel mutations also allows for the development of transgenic pigs bearing mutations in these enzymes or for genetic screening to identify pigs on the basis of their CYP17 and/or CYB5 genotype. Pigs having these mutations may be selected and bred to produce pigs that have a lower incidence of boar taint.Type: ApplicationFiled: October 13, 2014Publication date: April 16, 2015Inventor: E. James Squires
-
Publication number: 20150105315Abstract: The present invention relates to polypeptide fragments comprising an amino-terminal fragment of the PA subunit of a viral RNA-dependent RNA polymerase or variants thereof possessing endonuclease activity, wherein said PA subunit is from a virus belonging to the Orthomyxoviridae family. This invention also relates to (i) crystals of the polypeptide fragments which are suitable for structure determination of said polypeptide fragments using X-ray crystallography and (ii) computational methods using the structural coordinates of said polypeptide to screen for and design compounds that modulate, preferably inhibit the endonucleolytically active site within the polypeptide fragment. In addition, this invention relates to methods identifying compounds that bind to the PA polypeptide fragments possessing endonuclease activity and preferably inhibit said endonucleolytic activity, preferably in a high throughput setting.Type: ApplicationFiled: October 21, 2014Publication date: April 16, 2015Applicants: EUROPEAN MOLECULAR BIOLOGY LABORATORY (EMBL), UNIVERSITY JOSEPH FOURIER FILIALE, Centre National de la Recherche Scientifique - Direction de la Politique IndustrielleInventors: Denis Bouvier, Thibaut Crepin, Rob Ruigrok, Alexander Dias, Stephen Cusack
-
Publication number: 20150104437Abstract: The present invention relates to methods of the treatment and diagnosis of bone mineral density related disorders. More particularly, the present invention relates to a method of diagnosing or predicting a hone mineral density related disease, or a risk of a bone mineral density related disease, in a subject, which method comprises detecting a mutation in the TBXAS1 gene, wherein the presence of said mutation is indicative of a bone mineral density related disease or of a risk of a bone mineral density related disease. The invention also relates to a compound selected in the group consisting of a thromboxane synthase (TXAS) encoding polynucleotide, a TXAS, thromboxane A2 or an analog thereof for treating or preventing a disease associated with an increased bone mineral density (e.g., Ghosal hematodiaphyseal dysplasia syndrome).Type: ApplicationFiled: December 19, 2014Publication date: April 16, 2015Inventors: Valerie Cormier-Daire, Arnold Munnich
-
Publication number: 20150104837Abstract: The present invention relates to a novel isolated gene (polynucleotide) which encodes a protein having a biofilm formation inhibitory activity derived from Coryneform bacteria, a L-lysine-producing strain in which the polynucleotide is inactivated, and a method for producing L-lysine using the same.Type: ApplicationFiled: October 14, 2014Publication date: April 16, 2015Inventors: Un Hwan HA, Yong Jae KIM, Jung Hoon LEE, Hee Sung SHIN, Jun Ok MOON, Hyung Joon KIM, Kwang Ho LEE
-
Publication number: 20150104790Abstract: Compositions and methods are provided for improved reverse transcriptases and their uses in reverse transcription where the improvement may include increased temperature, increased salt, increased activity and/or increased dUTP tolerance.Type: ApplicationFiled: October 16, 2014Publication date: April 16, 2015Applicant: NEW ENGLAND BIOLABS, INC.Inventors: Yinhua Zhang, Thomas C. Evans, JR.
-
Publication number: 20150104433Abstract: The present invention relates to a method for preparing an expression vector encoding a tailored recombinase, which tailored recombinase is capable of recombining asymmetric target sequences within the long terminal repeat (LTR) of proviral DNA of a plurality of retrovirus strains inserted into the genome of a host cell, as well as to the obtained expression vector, cells transfected with this, expressed recombinase and pharmaceutical compositions comprising the expression vector, cells and/or recombinase. Pharmaceutical compositions are useful, e.g., in treatment and/or prevention of retrovirus infection. In particular, asymmetric target sequences present in a plurality of HIV strains are disclosed, as well as tailored recombinases capable of combining these sequences (Tre 3.0 and 4.0) and expression vectors encoding them.Type: ApplicationFiled: December 4, 2014Publication date: April 16, 2015Inventors: Joachim Hauber, Jan Chemnitz, Frank Buchholz, Janet Chusainow
-
Publication number: 20150104851Abstract: The present invention relates to novel mutants with cyclase activity and use thereof in a method for biocatalytic cyclization of terpenes, such as in particular for the production of isopulegol by cyclization of citronellal; a method for the preparation of menthol and methods for the biocatalytic conversion of further compounds with structural motifs similar to terpene.Type: ApplicationFiled: December 4, 2014Publication date: April 16, 2015Inventors: Michael Breuer, Bernhard Hauer, Dieter Jendrossek, Gabrielle Siedenburg, Juergen Pleiss, Demet Sirim, Silvia Racolta
-
Publication number: 20150104841Abstract: The present invention provides isolated gene sequences involved in xylose fermentation and related recombinant yeast which are useful in methods of enhanced biofuel production, particularly ethanol production. Methods of bioengineering recombinant yeast useful for biofuel production are also provided.Type: ApplicationFiled: June 17, 2014Publication date: April 16, 2015Inventors: Dana J. Wohlbach, Audrey P. Gasch
-
Patent number: 9006514Abstract: This invention is in the field of biotechnology, in particular, this pertains to polynucleotide sequences encoding membrane bound O-acyltransferase genes and the use of these acyltransferases for altering fatty acid profiles in oilseed plants. Methods for increasing elongation and desaturation conversion efficiencies are also disclosed.Type: GrantFiled: December 19, 2013Date of Patent: April 14, 2015Assignee: E. I. du Pont de Nemours and CompanyInventors: Howard Glenn Damude, Knut Meyer
-
Patent number: 9006408Abstract: The invention provides compounds, pharmaceutical compositions and methods for treating atherosclerosis, coronary heart disease, thrombosis, and for decreasing or prevention of accumulation of cholesterol in a subject by modifying LCAT polypeptide.Type: GrantFiled: March 4, 2014Date of Patent: April 14, 2015Assignee: Amgen Inc.Inventors: Mingyue Zhou, Wenyan Shen, Bei Shan, Margrit Schwarz, David Park Meininger, Tom Boone