Encodes An Enzyme Patents (Class 536/23.2)
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Publication number: 20120095086Abstract: Tyrosine phosphorylation, regulated by protein tyrosine phosphatases (PTPs) and kinases (PTKs), is important in signaling pathways underlying tumorigenesis. A mutational analysis of the tyrosine phosphatase gene superfamily in human cancers identified 83 somatic mutations in six PTPs (PTPRF, PTPRG, PTPRT, PTPN3, PTPN13, PTPN14), affecting 26% of colorectal cancers and a smaller fraction of lung, breast and gastric cancers. Fifteen mutations were nonsense, frameshift or splice site alterations predicted to result in truncated proteins lacking phosphatase activity. Five missense mutations in the most commonly altered PTP (PTPRT) were biochemically examined and found to reduce phosphatase activity. Expression of wild-type but not a mutant PTPRT in human cancer cells inhibited cell growth. These observations suggest that the tyrosine phosphatase genes are tumor suppressor genes, regulating cellular pathways that may be amenable to therapeutic intervention.Type: ApplicationFiled: October 18, 2011Publication date: April 19, 2012Applicant: THE JOHNS HOPKINS UNIVERSITYInventors: Zhenghe WANG, Victor VELCULESCU, Kenneth W. KINZLER, Bert VOGELSTEIN
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Publication number: 20120094335Abstract: The subject invention pertains to a novel purified polypeptide having laccase activity and the nucleic acid sequences encoding the polypeptide. The disclosed polypeptide works at moderately high temperatures from below 20° C. to about 70° C., both acidic and alkaline pH conditions, high salt concentrations and in the presence of organo solvents. The high stability of the enzyme enables its wide applications under even extreme conditions. The invention also provides methods of producing the laccase enzyme.Type: ApplicationFiled: May 10, 2010Publication date: April 19, 2012Applicant: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION,INC.Inventors: Julie A. Maupin-Furlow, Sivakumar Uthandi, Boutaiba Saad, Matthew A. Humbard
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Publication number: 20120096592Abstract: The present invention relates generally to the field of molecular biology and concerns a method for enhancing yield related traits by modulating expression in a plant of a nucleic acid encoding a BET1-like polypeptide. The present invention also concerns plants having modulated expression of a nucleic acid encoding this BET1-like polypeptide, which plants have enhanced yield-related traits relative to corresponding wild type plants or other control plants. The invention also provides constructs useful in the methods of the invention. The present invention relates generally to the field of molecular biology and concerns a method for improving various plant growth characteristics by modulating expression in a plant of a nucleic acid encoding a CRT (Calreticulin). The present invention also concerns plants having modulated expression of a nucleic acid encoding a Calreticulin, which plants have improved growth characteristics relative to corresponding wild type plants or other control plants.Type: ApplicationFiled: February 19, 2010Publication date: April 19, 2012Applicant: BASF Plant Science Company GmbHInventors: Yves Hatzfeld, Ana Isabel Sanz Molinero, Valerie Frankard, Christophe Reuzeau
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Publication number: 20120096600Abstract: The present invention relates to plants with a chimeric DNA molecule encoding a glyphosate tolerant EPSPS enzyme under the control of a plant constitutive promoter and a replacement histone intron 1, thereby conferring enhanced glyphosate tolerance to said plants.Type: ApplicationFiled: June 24, 2010Publication date: April 19, 2012Inventors: Rene Ruiter, Frank Meulewaeter, Chantal Vanderstraeten
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Publication number: 20120094345Abstract: The invention provides a non-naturally occurring microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (1,4-BDO) biosynthetic pathways. The pathways include exogenous nucleic acids encoding a) an ?-ketoglutarate decarboxylase; b) a 4-hydroxybutanoate dehydrogenase; c) a 4-hydroxybutyryl-CoA:acetyl-CoA transferase or a butyrate kinase and a phosphotransbutyrylase; d) an aldehyde dehydrogenase, and e) an alcohol dehydrogenase, wherein the exogenous nucleic acids are expressed in sufficient amounts to produce 1,4-butanediol (1,4-BDO). Also provide is a method for the production of 1,4-BDO. The method includes culturing the non-naturally occurring microbial organism having 4-HB and 1,4-BDO biosynthetic pathways substantially anaerobic conditions for a sufficient period of time to produce 1,4-BDO.Type: ApplicationFiled: October 31, 2011Publication date: April 19, 2012Applicant: Genomatica, Inc.Inventors: Mark J. Burk, Stephen J. Van Dien, Anthony P. Burgard, Wei Niu
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Patent number: 8158392Abstract: The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desaturase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids.Type: GrantFiled: December 5, 2011Date of Patent: April 17, 2012Assignee: Commonwealth Scientific and Industrial Research OrganisationInventors: Surinder Pal Singh, Stanley Suresh Robert, Peter David Nichols, Susan Irene Ellis Blackburn, Xue-Rong Zhou, James Robertson Petrie, Allan Graham Green
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Patent number: 8158362Abstract: Polymorphisms in the FLAP and LTA4H gene are shown by genetic association analysis to be susceptibility markers for myocardial infarction (MI) and ACS, as well as stroke and PAOD. Pathway targeting for treatment and diagnostic applications in identifying those who are at risk of developing MI, ACS, stroke or PAOD, in particular are described. The invention also provides methods of prophylaxis therapy for MI in human subjects having a race including black African ancestry by administering to the subject a composition comprising a therapeutically effective amount of MI therapeutic agent that inhibits leukotriene synthesis in vivo. The invention also provides for compositions comprising a leukotriene synthesis inhibitor and a statin and methods of using these compositions to reduce C-reactive protein in a human subject at risk of MI, ACS, stroke and/or PAOD.Type: GrantFiled: November 9, 2005Date of Patent: April 17, 2012Assignee: deCODE Genetics ehf.Inventors: Anna Helgadottir, Hákon Hákonarson, Jeffrey R. Gulcher, Mark E. Gurney
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Patent number: 8158404Abstract: The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.Type: GrantFiled: March 30, 2011Date of Patent: April 17, 2012Assignee: Gevo, Inc.Inventors: Doug Lies, Stephanie Porter-Scheinman, Julie Kelly, Catherine Asleson Dundon, Aristos Aristidou, Andrew Hawkins
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Patent number: 8158391Abstract: A substantially pure Candida host cell for the production of a ?-carboxyl-?-hydroxy fatty acid having a carbon chain length in the range from C6 to C22, a ?,?-dicarboxylic fatty acid having a carbon chain length in the range from C6 to C22, or mixtures thereof, is provided. The Candida host cell is characterized by a first genetic modification class and a second genetic modification class. The first genetic modification class comprises one or more genetic modifications that disrupt the peroxisomal ?-oxidation pathway. The second genetic modification class comprises one or more genetic modifications that collectively or individually disrupt at least one gene selected from the group consisting of a CYP52A type cytochrome P450, a fatty alcohol oxidase, and an alcohol dehydrogenase.Type: GrantFiled: May 6, 2009Date of Patent: April 17, 2012Assignee: DNA Twopointo, Inc.Inventors: Richard A. Gross, Wenhua Lu, Jon Ness, Jeremy Minshull
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Patent number: 8158390Abstract: The invention relates to a method for producing L-valine and to a suitable microorganism. The inventive method is characterized by preferably enhancing the transaminase C activity of a coryneform bacterium, especially Corynebacterium glutamicum. The organisms so modified have a yield in L-valine which is 35.8% higher than that of non-modified organisms.Type: GrantFiled: August 19, 2005Date of Patent: April 17, 2012Assignee: Forschungszentrum Julich GmbHInventors: Jan Marienhagen, Lothar Eggeling, Hermann Sahm
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Patent number: 8158394Abstract: Bi-cistronic plasmids used for the expression of formate dehydrogenase (FDH) and modified phenylalanine dehydrogenase (PDHmod) are provided.Type: GrantFiled: April 8, 2010Date of Patent: April 17, 2012Assignee: Bristol-Myers Squibb CompanyInventors: Jonathan Basch, Thomas Franceschini, Suo Win Liu, Shu-Jen Chiang
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Patent number: 8158397Abstract: The present invention provides a novel cellulase nucleic acid sequence, designated 029cel, and the corresponding 029cel amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding 029cel, recombinant 029cel proteins and methods for producing the same.Type: GrantFiled: August 18, 2009Date of Patent: April 17, 2012Assignee: Danisco US Inc.Inventors: Brian E. Jones, William D. Grant, Shaun Heaphy, Susan Grant
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Patent number: 8158382Abstract: Invention relates to novel and improved UBP1 protease mutants with a substitution at position (754), a deletion of amino-acids at position (1-54) and at least a portion of the amino-acids found at position (55-98) and their coding sequence as well as their applications and heterogonous protein expression system comprising thereof.Type: GrantFiled: January 10, 2005Date of Patent: April 17, 2012Assignee: Instytut Biotechnologii I AntybiotykowInventors: Andrzej Plucienniczak, Anna Wojtowicz, Anna Mazurkiewicz, Luiza Chojnacka
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Publication number: 20120088280Abstract: An isolated polynucleotide encoding for a polypeptide comprising an amino acid sequence set forth in SEQ ID NO: 1 with polymer synthase activity.Type: ApplicationFiled: May 5, 2010Publication date: April 12, 2012Inventors: K.Sudesh Kumar C.Kanapathi Pillai, Mohammed Razip Bin Samian, Amirul Al-Ashraf Balakrishnan Bin Abdullah, Kesivan Bhubalan
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Publication number: 20120088288Abstract: The present invention relates to methods for producing variants of a parent TY145 subtilase and of a parent BPN? subtilase and to TY145 and BPN? variants having altered properties as compared to the parent TY145/BPN? subtilase.Type: ApplicationFiled: November 28, 2011Publication date: April 12, 2012Applicant: NOVOZYMES A/SInventors: Allan Svendsen, Henriette Draborg
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Publication number: 20120088237Abstract: A restriction endonuclease is provided that has been engineered to have a cleavage specificity for a DNA recognition sequence containing a modified nucleotide. Methods for engineering enzymes to cleave DNA containing modified nucleotides at specific sequences are provided.Type: ApplicationFiled: March 4, 2010Publication date: April 12, 2012Applicant: NEW ENGLAND BIOLABS, INC.Inventors: Zhenyu Zhu, Shengxi Guan, Aine Quimby
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Publication number: 20120090044Abstract: The nucleotide and amino acid sequences of indoleamine 2,3-dioxygenase-2 (IDO2) and methods of use thereof are provided.Type: ApplicationFiled: November 4, 2011Publication date: April 12, 2012Inventors: George C. Prendergast, Richard Metz
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Publication number: 20120090052Abstract: The present invention relates generally to the field of molecular biology and concerns a method for enhancing various yield-related traits and/or plant growth characteristics in plants by modulating expression in a plant of a nucleic acid encoding a C3H-like polypeptide, or a SPATULA-like (SPT) polypeptide, or an IDI2 (Iron Deficiency Induced 2) polypeptide, or an eIF4F-like protein complex subunit, or GR-RBP (Glycine Rich-RNA Binding Protein) polypeptide. The present invention also concerns plants having modulated expression and/or activity of a nucleic acid encoding a C3H-like polypeptide, or a SPATULA-like (SPT) polypeptide, or an IDI2 (Iron Deficiency Induced 2) polypeptide, or an eIF4F-like protein complex subunit, or GR-RBP (Glycine Rich-RNA Binding Protein) polypeptide, which plants have enhanced yield-related traits and/or plant growth characteristics relative to corresponding wild type plants or other control plants. The invention also provides constructs useful in the methods of the invention.Type: ApplicationFiled: April 27, 2010Publication date: April 12, 2012Inventors: Ana Isabel Sanz Molinero, Valerie Frankard, Yves Hatzfeld
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Publication number: 20120090043Abstract: The invention concerns an isolated complex comprising an HIV or HTLV protein and a human protein. Corresponding nucleic acids, vectors, host cells, host organisms, compositions, kits, medical uses, diagnostic uses, and methods of screening agents are also contemplated. Disclosed are 212 interactions between 19 retroviral proteins and 131 human proteins.Type: ApplicationFiled: June 30, 2010Publication date: April 12, 2012Applicant: UNIVERSITE DE LIEGEInventors: Jean-Claude Twizere, Nicolas Simonis
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Publication number: 20120088281Abstract: The present invention relates to a modified thiolase protein with an improved activity, a polynucleotide encoding the modified protein, an expression vector including the polynucleotide, and a transformant, to a composition for producing a biobutanol including the thiolase with an improved activity or a cell expressing the thiolase, and to a method of producing the biobutanol.Type: ApplicationFiled: October 5, 2011Publication date: April 12, 2012Applicant: POSTECH ACADEMY-INDUSTRY FOUNDATIONInventor: Kyung Jin KIM
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Publication number: 20120090042Abstract: The present invention relates to polypeptides having phytase activity. These polypeptides have an amino acid sequence which has at least 70% identity to either of three phytases derived from the bacterium Buttiauxella, and which comprises at least one of the following amino acids at the position indicated: 119N, 120L, and/or 121E. These phytases have an improved specific activity. Additional specific amino acid substitutions are also disclosed which characterize and distinguish additional phytases of the invention having improved properties such as temperature and/or pH stability, pH activity profile, temperature activity profile, substrate profile, improved performance in animal feed in vitro or in vivo. The invention also relates to isolated polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: ApplicationFiled: December 16, 2011Publication date: April 12, 2012Applicant: NOVOZYMES A/SInventors: Carsten Sjoeholm, Soeren Flensted Lassen, Lars Kobberoe Skov, Leonardo De Maria
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Publication number: 20120087921Abstract: Novel polypeptides comprising a chemokine-binding peptide and an Fc fragment are disclosed. The polypeptides are capable of binding to certain chemokines so as to modulate their activity. These polypeptides can be used to modulate in vivo chemokine-dependent processes such as inflammation and autoimmunity, and to treat associated conditions.Type: ApplicationFiled: June 15, 2010Publication date: April 12, 2012Applicant: Biokine Therapeutics Ltd.Inventors: Michal Abraham, Orly Eizenberg, Amnon Peled
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Publication number: 20120090054Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: ApplicationFiled: December 19, 2011Publication date: April 12, 2012Applicants: Novozymes, Inc., Novozymes A/SInventors: Paul Harris, Elena Valsenko, Elizabeth Zaretsky, Marcus Sakari Kauppinnen
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Publication number: 20120088273Abstract: The invention relates to a lignocellulose active protein comprising a DUF1996 domain and proteins that additionally comprise a CBM1 domain. Also included in the invention are proteins with a new domain, characterized by the consensus sequence [GA]-[ST]-[IV]-[ILV]-W-[DS]-G-[RIFS]-F-[ND]-[DS]-X (residues 1-12 of SEQ ID NOS:6-7). The invention relates to a lignocellulose active protein comprising a DUF1996 domain and proteins that additionally comprise a CBM1 domain.Type: ApplicationFiled: March 17, 2010Publication date: April 12, 2012Applicant: Nederlandse Organisatie Voor Toegepast- Natuurwetenschappelijk Onderzoek TnoInventor: Peter Jan Punt
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Publication number: 20120090051Abstract: Plant nitrogen use efficiency in corn has been improved by transformation with a flavohemoglobin gene. Plants comprising a flavohemoglobin gene have decreased nitric oxide (NO) levels, increased biomass accumulation under a sufficient nitrogen growth condition, and increased chlorophyll content under a limiting nitrogen growth condition. Additionally, these transformed plants evidence higher levels of yield.Type: ApplicationFiled: October 4, 2010Publication date: April 12, 2012Inventors: Amarjit Basra, Mike Edgerton, Garrett J. Lee, Maolong Lu, Linda L. Lutfiyya, Wei Wu, Xiaoyun Wu
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Publication number: 20120088277Abstract: The invention relates to a variant of a parent fungal glucoamylase, which exhibits improved thermal stability and/or increased specific activity using saccharide substrates.Type: ApplicationFiled: December 19, 2011Publication date: April 12, 2012Applicant: NOVOZYMES A/SInventors: Bjarne Roenfeldt Nielsen, Allan Svendsen, Henrik Pedersen, Jesper Vind, Hanne Vang Hendriksen, Torben Peter Frandsen
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Patent number: 8153391Abstract: Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.Type: GrantFiled: August 29, 2008Date of Patent: April 10, 2012Assignee: Bunge Oils, Inc.Inventors: Christopher L. G. Dayton, Tim Hitchman, Katie Kline, Jonathan Lyon, Mark A. Wall, Nelson R. Barton
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Patent number: 8153415Abstract: The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.Type: GrantFiled: March 31, 2011Date of Patent: April 10, 2012Assignee: Gevo, Inc.Inventors: Thomas Buelter, Andrew Hawkins, Stephanie Porter-Scheinman, Peter Meinhold, Catherine Asleson Dundon, Aristos Aristidou, Jun Urano, Doug Lies, Matthew Peters, Melissa Dey, Justas Jancauskas, Julie Kelly, Ruth Berry
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Patent number: 8153413Abstract: The invention aims to provide a novel alkaline protease having peculiar properties such as high alkali activity, resistance to surfactants and calcium-dependent thermostability and exhibiting excellent performance in highly alkaline detergents, and a gene coding for the amino acid sequence thereof. There is provided an alkaline protease with such properties that an active pH range is from 5 to 13, an optimum pH is approximately 12.6, an optimum temperature is 70° C., no activity drop by heating is observed up to 65° C. at pH 10 and the optimum temperature and the thermostability are not affected by Ca2+ ions. Specifically, there is provided, for example, an alkaline protease having an amino acid sequence constituting a mature enzyme as represented by SEQ ID NO: 3 or an amino acid sequence resulting from deletion, substitution, situs inversus arrangement, addition or insertion of a part of amino acids thereof, or derived from Alkaliphillus transvaalensis.Type: GrantFiled: November 16, 2005Date of Patent: April 10, 2012Assignee: Japan Agency for Marine-Earth Science and TechnologyInventors: Jie Lu, Zhi jun Li, Si Hung Vo, Yuji Hatada, Ken Takai, Susumu Ito, Koki Horikoshi
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Patent number: 8153411Abstract: The invention relates to haloalkane dehalogenases and to polynucleotides encoding the haloalkane dehalogenases. In addition methods of designing new dehalogenases and method of use thereof are also provided. The dehalogenases have increased activity and stability at increased pH and temperature.Type: GrantFiled: January 7, 2010Date of Patent: April 10, 2012Assignee: Verenium CorporationInventors: Jay M. Short, Toby Richardson, Dan E. Robertson, Kevin Gray
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Patent number: 8153412Abstract: Variants of Bacillus sp. TS-23 strain alpha-amylases exhibit improved enzymatic performance, including increased themostability, reduced calcium dependence, increased washing/cleaning performance, and baking ability. Compositions comprising these variants are useful in methods of starch processing, starch liquefaction, fermatation, starch saccharification, cleaning, laundrying, textile desizing, baking, and biofilm removal.Type: GrantFiled: November 3, 2008Date of Patent: April 10, 2012Assignee: Danisco US Inc.Inventors: Claudine Y. Chang, Clement Choy, Melodie Estabrook, Mansi Goyal, Thomas P. Graycar, Victoria E. Huang, Brian E. Jones, Marc Kolkman, Karsten M. Kragh, Chris Leeflang, Scott D. Power, Sandra W. Ramer, Andrew Shaw, Casper Vroemen, Walter Weyler
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Publication number: 20120082976Abstract: Disclosed are a novel method of screening for insulin secretion-potentiating agents as well as means for performing such screening. The means include a DNA encoding fluorescent-labeled Epac2 comprising two different DNAs encoding two different fluorescent proteins which emit fluorescent light with wavelength differing from each other and a DNA encoding Epac2 which are fused together in-frame, and the cells transformed with the DNA. Also disclosed is a method of screening insulin secretion-potentiating agents comprising bringing a candidate compound into contact with cells transformed with the said DNA, and detecting whether the compound binds to Epac2.Type: ApplicationFiled: April 15, 2010Publication date: April 5, 2012Applicant: KOBE UNIVERSITYInventors: Susumu Seino, Changliang Zhang, Megumi Kato, Tadao Shibazaki
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Publication number: 20120082983Abstract: Compositions and methods are provided that relate to the bioremediation of chlorinated ethenes, particularly the bioremediation of vinyl chloride by Dehalococcoides-like organisms. An isolated strain of bacteria, Dehalococcoides sp. strain VS, that metabolizes vinyl chloride is provided; the genetic sequence of the enzyme responsible for vinyl chloride dehalogenation; methods of assessing the capability of endogenous organisms at an environmental site to metabolize vinyl chloride; and a method of using the strains of the invention for bioremediation.Type: ApplicationFiled: November 11, 2011Publication date: April 5, 2012Inventors: Alfred M. Spormann, Jochen A. Muller, Bettina M. Rosner, Gregory Von Abendroth, Galit Meshulam-Simon, Perry L. McCarty
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Publication number: 20120083024Abstract: The subject invention concerns polynucleotides encoding a plant 2-phenylethanol dehydrogenase enzyme. In one embodiment, the polynucleotide encodes a tomato 2-phenylethanol dehydrogenase. The subject invention also concerns polynucleotides encoding a plant phenylalanine decarboxylase enzyme. In one embodiment, the polynucleotide encodes a tomato phenylalanine decarboxylase. The subject invention also concerns 2-phenylethanol dehydrogenase polypeptides and phenylalanine decarboxylase polypeptides encoded by polynucleotides of the present invention. The subject invention also concerns methods for providing a plant with an increased flavor and aroma volatile. Plants can be transformed with one or more polynucleotide of the present invention. The subject invention also concerns these transformed plant cells, plant tissue, and plants and transgenic progeny thereof.Type: ApplicationFiled: October 1, 2004Publication date: April 5, 2012Inventors: Harry J. Klee, Denise Tieman
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Publication number: 20120083590Abstract: A polypeptide having a high fidelity DNA polymerase activity and thus being useful as a genetic engineering reagent; a gene encoding this polypeptide; a method of producing the polypeptide; and a method of amplifying a nucleic acid by using the polypeptide.Type: ApplicationFiled: September 22, 2011Publication date: April 5, 2012Applicant: Takara Bio Inc.Inventors: Yoshimi Sato, Kazue Nishiwaki, Nana Shimada, Shigekazu Hokazono, Takashi Uemori, Hiroyuki Mukai, Ikunoshin Kato
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Publication number: 20120084889Abstract: The invention provides isolated nucleic acid molecules which encodes a novel fatty acid nECR. The invention also provides recombinant expression vectors containing nECR nucleic acid molecules, host cells into which the expression vectors have been introduced, and methods for large-scale production of long chain polyunsaturated fatty acids (LCPUFAs), e.g., ARA, EPA and DHA.Type: ApplicationFiled: May 20, 2010Publication date: April 5, 2012Inventors: Johnathan A. Napier, Olga Sayanova, Frederic Beaudoin
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Publication number: 20120083013Abstract: The invention provides a polynucleotide encoding a furofuran lignan 4-O-glucosyltransferase, and others. The polynucleotide in accordance with the present invention comprises a polynucleotide consisting of the nucleotide sequence represented by SEQ ID NO: 3 or SEQ ID NO: 5, or a polynucleotide encoding a protein consisting of the amino acid sequence represented by SEQ ID NO: 4 or SEQ ID NO: 6.Type: ApplicationFiled: May 18, 2010Publication date: April 5, 2012Applicant: Suntory Holdings LimitedInventors: Eiichiro Ono, Honoo Satake, Hyun-Jung Kim
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Publication number: 20120084890Abstract: Expression cassettes causing specific regulatory control of transgene expression in plants, wherein the expression cassettes include regulatory sequences from the MADS gene family for expression of recombinant gene products in the reproductive tissue of plants for the purpose of generating abiotic stress tolerant plants.Type: ApplicationFiled: October 28, 2011Publication date: April 5, 2012Inventors: MICHAEL L. NUCCIO, L. MARK LAGRIMINI, MOEZ MEGHJI
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Patent number: 8148495Abstract: The present invention relates to methods for degrading or converting a cellulosic material and for producing a substance from a cellulosic material.Type: GrantFiled: July 16, 2009Date of Patent: April 3, 2012Assignee: Novozymes, Inc.Inventors: Paul Harris, Michael Rey, Hanshu Ding
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Patent number: 8148104Abstract: The invention relates to a xylanase originating from a Bacillus strain. This xylanase is active over a wide range of acid and basic pH. The invention also relates to new strains of microorganisms producing this xylanase and to methods for preparing this xylanase. The invention also relates to a DNA molecule and to an expression vector or an integration vector containing this DNA molecule. The invention also relates to uses of the latter and to compositions containing it.Type: GrantFiled: August 20, 2007Date of Patent: April 3, 2012Assignee: Danisco US Inc.Inventors: Eric De Buyl, Andrée Lahaye, Pierre Ledoux, René Detroz
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Patent number: 8148314Abstract: Disclosed herein is a method for stabilization of the perhydrolase activity of the CE-7 esterase in a formulation with a carboxylic acid ester that employs the addition of a buffering agent, substantially undissolved, to the mixture of the CE-7 esterase and the carboxylic acid ester. Further, disinfectant and laundry care formulations comprising the peracids produced by the processes described herein are provided.Type: GrantFiled: August 19, 2011Date of Patent: April 3, 2012Assignee: E. I. du Pont de Nemours and CompanyInventors: Robert Dicosimo, Arie Ben-Bassat, Mark S. Payne, Raymond Richard Zolandz
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Patent number: 8147842Abstract: An object of the present invention is to provide a mutant luciferase having luciferase activity with an altered emission spectrum. A specific amino acid residue(s) is substituted in a luciferase derived from Cypridina noctiluca and then the resulting mutant luciferase having luciferase activity with an emission spectrum differing from that of the wild-type luciferase is screened for.Type: GrantFiled: January 26, 2007Date of Patent: April 3, 2012Assignee: National Institute of Advanced Industrial Science and TechnologyInventors: Kosei Kawasaki, Yousuke Morita, Satoru Ohgiya, Yoshihiro Ohmiya, Yasushi Ohyama
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Patent number: 8148121Abstract: The present invention relates to ?6 desaturases, which have the ability to convert linoleic acid [“LA”; 18:2 ?-6] to ?-linolenic acid [“GLA”; 18:3 ?-6] and/or ?-linolenic acid [“ALA”; 18:3 ?-3] to stearidonic acid [“STA”; 18:4 ?-3]. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding ?6 desaturases, along with methods of making long-chain polyunsaturated fatty acids [“PUFAs”] using these ?6 desaturases in oleaginous yeast, are disclosed.Type: GrantFiled: July 30, 2009Date of Patent: April 3, 2012Assignee: E.I. du Pont de Nemours and CompanyInventors: Narendra S. Yadav, Zhixiong Xue, Quinn Qun Zhu, Hongxiang Zhang
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Patent number: 8148125Abstract: Compositions comprising truncated tryptophanyl-tRNA synthetase polypeptides useful for regulating angiogenesis, as well as nucleic acids encoding such tRNA synthetase polypeptides are described. Methods of making and using such compositions are also disclosed.Type: GrantFiled: January 17, 2011Date of Patent: April 3, 2012Assignee: The Scripps Research InstituteInventors: Paul Schimmel, Keisuke Wakasugi
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Patent number: 8148124Abstract: The invention relates to the cDNA and deduced amino acid sequence of the Coactivator Associated arginine (R) Methyltransferase protein, CARM1. A method is described for the use CARM1 to regulate gene expression in vivo. CARM1 has also been used to methylate arginine residues of histones, synthetic peptides, and other proteins. A method to use CARM1 to screen for drugs that inhibit its methyltransferase activity is also described, as is a method to screen for drugs that modulate CARM1's interactions with other proteins.Type: GrantFiled: April 17, 2007Date of Patent: April 3, 2012Assignees: The Regents of the University of California, University of Southern CaliforniaInventors: Michael R. Stallcup, Dagang Chen, Heng Hong, Dana W. Aswad
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Patent number: 8148316Abstract: Disclosed herein is a method for stabilization of the perhydrolase activity of the CE-7 esterase in a formulation with a carboxylic acid ester that employs the addition of a buffering agent, substantially undissolved, to the mixture of the CE-7 esterase and the carboxylic acid ester. Further, disinfectant and laundry care formulations comprising the peracids produced by the processes described herein are provided.Type: GrantFiled: August 19, 2011Date of Patent: April 3, 2012Assignee: E. I du Pont de Nemours and CompanyInventors: Robert DiCosimo, Arie Ben-Bassat, Mark S. Payne, Raymond Richard Zolandz
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Patent number: 8148127Abstract: The present invention relates to polypeptides having glucoamylase activity and isolated polynucleotides encoding said polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides. The invention also relates to the composition comprising a glucoamylase of the invention as well as the use such compositions for starch conversion processes, brewing, including processes for producing fermentation products or syrups.Type: GrantFiled: December 10, 2009Date of Patent: April 3, 2012Assignees: Novozymes A/S, Novozymes North America, Inc.Inventors: Hiroaki Udagawa, Sara Landvik, Michiko Ihara, Jiyin Liu, Chee Leong Soong, Eric Allain, Shiro Fukuyama
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Chemically modified mutant serine hydrolases show improved catalytic activity and chiral selectivity
Patent number: 8148128Abstract: This invention provides novel chemically modified mutant serine hydrolases that catalyze a transamidation and/or a transpeptidation and/or a transesterification reaction. The modified serine hydrolases have one or more amino acid residues in a subsite replaced with a cysteine, wherein the cysteine is modified by replacing the thiol hydrogen in the cysteine with a substituent group providing a thiol side chain comprising a moiety selected from the group consisting of a polar aromatic substituent, an alkyl amino group with a positive charge, and a glycoside. In particularly preferred embodiments, the substitutents include an oxazolidinone, a C1 to C15 alkyl amino group with a positive charge, or a glycoside.Type: GrantFiled: September 19, 2008Date of Patent: April 3, 2012Assignee: The Governing Council of the University of TorontoInventors: John Bryan Jones, Michael Dickman, Richard C. Lloyd -
Patent number: 8148119Abstract: A vitamin D3 hydroxylase is purified from Pseudonocardia autotrophica cell, and a primer is designed based on amino acid sequence obtained from hydroxylase. Subsequently, PCR is conducted using genomic DNA of Pseudonocardia autotrophica as a template to clone a gene for the vitamin D3 hydroxylase. By conducting a conversion reaction using a microorganism in which the vitamin D3 hydroxylase gene is expressed using a proper expression system, a hydroxide of vitamin D or the like (e.g., hydroxy vitamin D3) can be produced with high efficiency.Type: GrantFiled: May 18, 2007Date of Patent: April 3, 2012Assignees: Microbiopharm Japan Co., Ltd., National Institute of Advanced Industrial Science and TechnologyInventors: Yoshikazu Fujii, Tomohiro Tamura
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Patent number: 8148100Abstract: The present invention comprises a method for assaying oxygenase activity the method comprising monitoring oxygenase activity of Mina53.Type: GrantFiled: September 25, 2006Date of Patent: April 3, 2012Assignee: ISIS Innovation LimitedInventors: Christopher Joseph Schofield, Michael Arnold McDonough, Nicolas Joseph Jean Granatino