Encodes An Enzyme Patents (Class 536/23.2)
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Publication number: 20120077204Abstract: Methods and compositions are provided for detecting molecular translocations, particularly protein translocations within and between sub-cellular compartments, using at least two components that exhibit a localization-dependent difference in complementation activity. In particular, alpha-complementing ?-galactosidase fragments are provided. These ?-galactosidase reporter fragments display significantly enhanced enzymatic activity when one fragment is localized in a membrane. Methods for carrying out no-wash ELISA assays based on the reporter component system are also provided.Type: ApplicationFiled: September 20, 2011Publication date: March 29, 2012Applicant: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Helen M. Blau, Thomas S. Wehrman
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Publication number: 20120077713Abstract: The present disclosure enables methods of identifying the VH and VL class pairs in the human immune repertoire, determining the VH and VL class pairs that are most prevalent and those having favorable biophysical properties. More specifically, the collections of the present disclosure comprise the most prevalent and/or preferred VH and VL class pairings with highly diversified CDRs.Type: ApplicationFiled: May 29, 2010Publication date: March 29, 2012Applicant: MORPHOSYS AGInventors: Markus Enzelberger, Stefanie Urlnger, Thomas Tiller, Josef Prassler, Tanja Herrmann
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Publication number: 20120077224Abstract: This disclosure concerns compositions and methods for improving the incorporation of unnatural amino acids (UAAs) into proteins in eukaryotic cells. It is shown herein that mutation of a prokaryotic tRNA synthetase to increase the interaction with the corresponding tRNA anticodon region results in increased UAA incorporation efficiency in mammalian cells.Type: ApplicationFiled: June 4, 2010Publication date: March 29, 2012Applicant: The Salk Institute for Biological StudiesInventor: Lei Wang
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Patent number: 8143482Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: GrantFiled: March 9, 2007Date of Patent: March 27, 2012Assignees: Novozymes, Inc., Novozymes AlsInventors: Søren Flensted Lassen, Paul Harris, Elena Vlasenko, Kristian Bertel Romer Morkeberg Krogh
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Patent number: 8143486Abstract: The subject invention relates to the identification of genes involved in the desaturation of polyunsaturated fatty acids at carbon 5 (i.e., “?5-desaturase”) and at carbon 6 (i.e., “?6-desaturase”) and to uses thereof. In particular, ?5-desaturase may be utilized, for example, in the conversion of dihomo-?-linolenic acid (DGLA) to arachidonic acid (AA) and in the conversion of 20:4n-3 to eicosapentaenoic acid (EPA). Delta-6 desaturase may be used, for example, in the conversion of linoleic (LA) to ?-linolenic acid (GLA). AA or polyunsaturated fatty acids produced therefrom may be added to pharmaceutical compositions, nutritional compositions, animal feeds, as well as other products such as cosmetics.Type: GrantFiled: September 9, 2010Date of Patent: March 27, 2012Assignee: Abbott LaboratoriesInventors: Pradip Mukerji, Tapas Das, Yung-Sheng Huang, Jennifer Thurmond, Suzette L. Pereira
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Patent number: 8143050Abstract: The invention relates to recombinant expression of a variant form of a fungal C1 strain ?-glucosidase. The invention also relates to the generation of fermentable sugars from biomass and the production of biofuels by fermentation of the sugars using genetically modified organisms expressing the ?-glucosidase variant. The invention provides methods for producing a fermentable sugar, such as glucose, from cellobiose by contacting cellobiose with a recombinant ?-glucosidase variant protein, such as a variant protein secreted by a recombinant host cell into culture medium. Methods of the invention may be used for conversion of a biomass substrate to a fermentable sugar, and ultimately to ethanol or other biofuel.Type: GrantFiled: November 24, 2010Date of Patent: March 27, 2012Assignee: Codexis, Inc.Inventors: Jie Yang, Xiyun Zhang, Attila Andor
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Patent number: 8143047Abstract: The invention relates to functional polypeptides secreted from Botryospaeria rhodina CBS 274.96.Type: GrantFiled: December 4, 2009Date of Patent: March 27, 2012Assignee: Novozymes AlsInventors: Kirk Matthew Schnorr, Lene Lange, Pernille Uldall Bolvig
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Patent number: 8143383Abstract: The subject invention relates to the identification of genes involved in the desaturation of polyunsaturated fatty acids at carbon 5 (i.e., “?5-desaturase”) and at carbon 6 (i.e., “?6-desaturase”) and to uses thereof. In particular, ?5-desaturase may be utilized, for example, in the conversion of dihomo-?-linolenic acid (DGLA) to arachidonic acid (AA) and in the conversion of 20:4n-3 to eicosapentaenoic acid (EPA). Delta-6 desaturase may be used, for example, in the conversion of linoleic (LA) to ?-linolenic acid (GLA). AA or polyunsaturated fatty acids produced therefrom may be added to pharmaceutical compositions, nutritional compositions, animal feeds, as well as other products such as cosmetics.Type: GrantFiled: September 9, 2010Date of Patent: March 27, 2012Assignee: Abbott LaboratoriesInventors: Pradip Mukerji, Tapas Das, Yung-Sheng Huang, Jennifer Thurmond, Suzette L. Pereira
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Patent number: 8143473Abstract: Transgenic soybean seed having increased total fatty acid content of at least 10% and altered fatty acid profiles when compared to the total fatty acid content of non-transgenic, null segregant soybean seed are described. DGAT genes from Yarrowia lipolytica are used to achieve the increase in seed storage lipids.Type: GrantFiled: May 23, 2008Date of Patent: March 27, 2012Assignee: E. I. du Pont de Nemours and CompanyInventors: Knut Meyer, William D. Hitz, Narendra S. Yadav, Howard G. Damude
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Patent number: 8143485Abstract: The present invention relates to plants, seeds and products derived thereof, in particular to Brassica plants, seeds products derived thereof, that have mutant sequences conferring high oleic acid profile on the seed oil. More particularly, the invention relates to mutant delta-12 fatty acid desaturase sequences, also referred to herein as FAD2 sequences, —in-such-plants which confer high oleic acid profile on the seed oil.Type: GrantFiled: March 21, 2007Date of Patent: March 27, 2012Assignee: Monsanto S.A.S.Inventors: Jean-Pierre Despeghel, Kunsheng Wu, Nelly Guguin
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Patent number: 8143036Abstract: Genetically modified microorganisms that produce itaconic acid at high yields and uses thereof.Type: GrantFiled: May 11, 2009Date of Patent: March 27, 2012Assignee: Industrial Technology Research InstituteInventors: James C. Liao, Pei-Ching Chang
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Patent number: 8143044Abstract: Provided are the expression method of influenza virus polymerase PAc-PB1N complex, the co-crystallization method of the complex and the three-dimendional structure of the crystal of PAc-PB1N complex. Also provided are the compounds binding to the influenza virus polymerase PAc and the expression method of influenza virus polymerase PAN. The three-dimensional structure of the crystal of PAc-PB1N complex can be used for screening and designing the drug for the treatment of influenza.Type: GrantFiled: February 22, 2009Date of Patent: March 27, 2012Assignee: Institute of Biophysics Chinese Academy of SciencesInventors: Yingfang Liu, Xiaojing He, Zonghao Zeng, Jie Zhou
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Patent number: 8143049Abstract: Provided are modified beta-glucosidase enzymes, derived from the Trichoderma reesei Cel3A beta-glucosidase, that exhibit improved stability at low pH, low pH and high aeration, low pH and high agitation, or low pH and elevated temperature. Also provided are genetic constructs comprising nucleotide sequences encoding for modified beta-glucosidase enzymes, methods for the production of modified beta-glucosidase enzymes from host strains and the use of the modified beta-glucosidase enzymes in the hydrolysis of cellulose.Type: GrantFiled: August 31, 2009Date of Patent: March 27, 2012Assignee: Iogen Energy CorporationInventors: Christopher Hill, James Lavigne, Martine Whissel, John J. Tomashek
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Patent number: 8143038Abstract: A thermophilic micro-organism comprising a modification that increases amylase expression and starch hydrolysis compared to wild-type, wherein the modification is insertion of a heterologous amylase gene.Type: GrantFiled: July 26, 2011Date of Patent: March 27, 2012Assignee: TMO Renewables LimitedInventors: Anthony Atkinson, Roger Cripps, Kirstin Eley, Brian Rudd, Martin Todd
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Patent number: 8143478Abstract: It is an object of the present invention to provide a means of imparting a yellowish flower color to a plant having no or little yellowish flower color or enhancing the yellowish flower color in a plant. In accordance with the present invention, a method of producing a plant having yellowish petals with the use of a peptide having transport activity to chromoplasts in petals and a fused gene formed of a gene encoding such peptide and one, or two or more genes encoding enzyme proteins involved in the carotenoid biosynthetic pathway is provided.Type: GrantFiled: November 29, 2005Date of Patent: March 27, 2012Assignee: Kirin Beer Kabushiki KaishaInventors: Naoyuki Umemoto, Toshihiro Toguri
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Publication number: 20120070876Abstract: The present invention relates to genetically modified proteins with uricolytic activity. More specifically, the invention relates to proteins comprising truncated urate oxidases and methods for producing them, including PEGylated proteins comprising truncated urate oxidases.Type: ApplicationFiled: September 7, 2011Publication date: March 22, 2012Applicant: Savient Pharmaceuticals, Inc.Inventors: Jacob Hartman, Simona Mendelovitz
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Publication number: 20120071546Abstract: The invention relates to a novel Glutamic Acid Decarboxylase (GAD). More specifically, novel DNA and protein sequences relating to GAD. Additionally, the invention discloses a novel composition and related methods for treating neurodegenerative diseases such as Parkinson's disease, Alzheimer's disease, epilepsy, and the like, using viral and non-viral delivery systems that deliver therapeutic agents to specific regions of the brain.Type: ApplicationFiled: November 29, 2011Publication date: March 22, 2012Applicant: NEUROLOGIX, INC.Inventors: Helen Fitzsimons, Ross Bland
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Publication number: 20120070881Abstract: The present invention relates to an isolated polynucleotide of the complete chromosome of Bacillus licheniformis. The present invention also relates to isolated genes of the chromosome of Bacillus licheniformis which encode biologically active substances and to nucleic acid constructs, vectors, and host cells comprising the genes as well as methods for producing biologically active substances encoded by the genes and to methods of using the isolated genes of the complete chromosome of Bacillus licheniformis.Type: ApplicationFiled: November 28, 2011Publication date: March 22, 2012Applicants: NOVOZYMES A/S, NOVOZYMES, INC.Inventors: Randy Berka, Michael Rey, Preethi Ramaiya, Jens Tonne Andersen, Michael Dolberg Rasmussen, Peter Bjarke Olsen
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Publication number: 20120073019Abstract: Compositions and methods for modulating plant development and for increasing yield in a plant are provided. The compositions include a ZM-CIPK1 sequence. Compositions of the disclosure comprise amino acid sequences and nucleotide sequences selected from SEQ ID NOS: 1 and 2 as well as variants and fragments thereof. Nucleotide sequences encoding the ZM-CIPK1 molecule are provided in DNA constructs for expression in a plant of interest are provided for modulating the level of a ZM-CIPK1 sequence in a plant or a plant part are provided. The methods comprise introducing into a plant or plant part a heterologous polynucleotide comprising a ZM-CIPK1 sequence of the disclosure. The level of the ZM-CIPK1 polypeptide can be increased or decreased. Such method can be used to increase the yield in plants; in one embodiment, the method is used to increase grain yield in cereals.Type: ApplicationFiled: September 19, 2011Publication date: March 22, 2012Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventor: Guofu Li
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Publication number: 20120070871Abstract: Highly productive D-lactic acid fermentation uses a transformant obtained by introducing into a host cell a polynucleotide encoding a polypeptide according to any one of the following (A) to (C) in such a manner that the polypeptide is expressed, which polypeptide has a D-lactate dehydrogenase activity higher than those of conventional polypeptides: (A) a polypeptide having the amino acid sequence shown in SEQ ID NO:1 or 2; (B) a polypeptide having the same amino acid sequence as shown in SEQ ID NO:1 or 2 except that one or several amino acids are substituted, deleted, inserted and/or added, which polypeptide has a D-lactate dehydrogenase activity; and (C) a polypeptide having an amino acid sequence which has a sequence identity of not less than 80% to the amino acid sequence shown in SEQ ID NO:1 or 2, which polypeptide has a D-lactate dehydrogenase activity.Type: ApplicationFiled: June 2, 2010Publication date: March 22, 2012Applicant: Toray Industries, Inc.Inventors: Kenji Sawai, Kazumi Suda, Hideki Sawai, Katsushige Yamada, Junya Yamagishi
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Publication number: 20120070863Abstract: The present invention provides a novel ?-galactoside-?2,3-sialyltransferase and a nucleic acid encoding the sialyltransferase. The present invention also provides a microorganism producing the sialyltransferase, as well as a method for producing the sialyltransferase using such a microorganism. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant ?-galactoside-?2,3-sialyltransferase. The present invention further provides a method for preparing a sialylsugar chain, which uses the sialyltransferase of the present invention.Type: ApplicationFiled: August 25, 2011Publication date: March 22, 2012Inventors: Takeshi YAMAMOTO, Hiroshi TSUKAMOTO, Yoshimitsu TAKAKURA
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Publication number: 20120070826Abstract: A novel gene (EPM2B) that is mutated in humans and dogs with Lafora's disease is described.Type: ApplicationFiled: January 14, 2011Publication date: March 22, 2012Inventors: Stephen W. Scherer, Berge A. Minassian
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Patent number: 8138392Abstract: The invention provides transgenic plants with resistance to infection by a root-infecting fungal plant pathogen such as Phymatotrichopsis omnivora. Also provided are methods of making such plants. Further provided are nucleic acid vectors for producing such a plant. Additionally, methods are provided for growing a dicotyledonous plant that is resistant to root rot disease in soil that comprises Phymatotrichopsis omnivora, or another pathogen.Type: GrantFiled: July 14, 2009Date of Patent: March 20, 2012Assignee: The Samuel Roberts Noble Foundation, Inc.Inventors: Srinivasa Rao Uppalapati, Kirankumar Mysore, Wensheng Li, Lloyd Sumner, Richard A. Dixon
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Patent number: 8137944Abstract: Variant polypeptides derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the variant polypeptides comprise an amino acid mutation at one or more positions selected from the group consisting of: 121, 161, 223, 146, 157, 158, 198, 229, 303, 306, 309, 316, 353, 26, 70, 145, 188, 272, 339, with reference to the position numbering of a Pseudomonas saccharophila exoamylase sequence shown as SEQ ID NO: 1.Type: GrantFiled: July 7, 2005Date of Patent: March 20, 2012Assignee: Danisco A/SInventors: Casper Tune Berg, Patrick M. F. Derkx, Carol Fioresi, Gijsbert Gerritse, Anja Hemmingen Kellet-Smith, Karsten Matthias Kragh, Wei Liu, Andrew Shaw, Bo Spange Sørensen, Charlotte Refdahl Thoudahl
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Patent number: 8137928Abstract: The present invention provides enhanced methods of producing soluble, active eukaryotic glycosyltransferases in prokaryotic microorganisms that have an oxidizing environment.Type: GrantFiled: March 24, 2006Date of Patent: March 20, 2012Assignee: BioGeneriXInventors: Marc F. Schwartz, Tarik Soliman
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Patent number: 8137940Abstract: The present invention relates to microorganisms genetically engineered to increase yield and/or efficiency of biomass production from a carbon source, such as e.g. glucose. Processes for generating such microorganisms are also provided by the present invention. The invention also relates to polynucleotide sequences comprising genes that encode proteins that are involved in the bioconversion of a carbon source such as e.g. glucose into biomass. The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. Also included are processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms leading to a microorganism with reduced carbon source diversion, i.e. higher yield and/or efficiency of biomass production from a carbon source such as e.g. glucose.Type: GrantFiled: September 7, 2006Date of Patent: March 20, 2012Assignee: DSM IP Assets B.V.Inventor: Masako Shinjoh
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Patent number: 8138393Abstract: Novel expression vectors and constructs encoding a chloroplast transit peptide (CTP) operably linked to a monomeric anthranilate synthase are provided. Additionally, novel polynucleotide sequences encoding monomeric anthranilate synthases are provided. Also provided are methods for increasing the levels of free tryptophan in transgenic plants containing the expression vectors and constructs.Type: GrantFiled: August 9, 2007Date of Patent: March 20, 2012Assignee: Monsanto Technology LLCInventors: Siva Manjunath, Santiago Xavier Navarro, William D. Rapp, Xiaohong Shi, Marguerite J. Varagona, Jennifer L. Winson, Guangning Ye
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Patent number: 8137950Abstract: A lactic acid bacterium (LAB) wherein an YjaE protein is essentially inactive and the LAB thereby get improved resistance to bacteriophages, a starter culture composition comprising the lactic acid bacterium and use of this starter culture manufacturing a food or feed product.Type: GrantFiled: January 6, 2006Date of Patent: March 20, 2012Assignee: Chr. Hansen A/SInventors: Birgitte Stuer-Lauridsen, Thomas Janzen
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Patent number: 8138324Abstract: The invention provides a novel system for the tunable expression of nucleic acids encoding e.g., polypeptides such as recombinant proteins in prokaryotic systems. The system is based on the ability of T7 lysozyme (T7Lys) to inhibit the activity of T7RNAP. Expression of T7Lys can be continuously adjusted as its expression is under the control of a promoter whose activity can be titrated. The invention provides a host cell capable of expressing T7 RNA polymerase, the host cell comprising a first nucleic acid having a T7 lysozyme gene or a T7 lysozyme variant gene and a tunable promoter for controlling the expression of the T7 lysozyme gene. It also provides a host cell further comprising a second nucleic acid having a T7 promoter operably linked to a nucleic acid sequence encoding a target polypeptide, whereby expression of the target polypeptide is tuned via controlling the expression of the T7 lysozyme gene.Type: GrantFiled: February 27, 2009Date of Patent: March 20, 2012Assignee: Xbrane Bioscience ABInventors: Jan Willem De Gier, Samuel Wagner
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Patent number: 8137925Abstract: The invention provides compositions and methods of use thereof for labeling peptide and proteins in vitro or in vivo. The methods described herein employ lipoic acid ligase or mutants thereof, and lipoic acid analogs recognized by lipoic acid ligase and lipoic acid ligase mutants.Type: GrantFiled: November 9, 2007Date of Patent: March 20, 2012Assignee: Massachusetts Institute of TechnologyInventors: Alice Y. Ting, Marta Fernandez Suarez, Hemanta Baruah
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Patent number: 8137974Abstract: The present invention is directed to the use of the maize Ac/Ds transposable elements in vertebrates.Type: GrantFiled: May 11, 2006Date of Patent: March 20, 2012Assignee: Temasek Life Sciences Laboratory LimitedInventors: Sergey Parinov, Alexander Emelyanov
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Patent number: 8137943Abstract: Thermostable DNA ligases with enhanced DNA binding activity and reaction activity are obtained. These modified thermostable DNA ligases having enhanced DNA binding activity compared to the wild type can be obtained by substituting a negatively charged amino acid (for example, the amino acid corresponding to the aspartic acid at position 540 of SEQ ID NO: 1) present at the N-terminal side of the C-terminal helix moiety of thermostable DNA ligases from thermophilic bacteria, hyperthermophilic bacteria, thermophilic archaea, or hyperthermophilic archaea, with a non-negatively charged amino acid (for example, alanine, serine, arginine, or lysine).Type: GrantFiled: February 23, 2009Date of Patent: March 20, 2012Assignee: Hitachi, Ltd.Inventors: Hirokazu Nishida, Maiko Tanabe
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Patent number: 8137951Abstract: The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating a Bacillus host cell under conditions suitable for production of the hyaluronic acid, wherein the Bacillus host cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6-phosphate isomerase gene.Type: GrantFiled: April 11, 2011Date of Patent: March 20, 2012Assignee: Novozymes, Inc.Inventors: Alan Sloma, Leslie Naggiar, legal representative, Regine Behr, William Widner, Maria Tang, David Sternberg, Linda Sternberg, legal representative, Stephen Brown
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Patent number: 8138321Abstract: A nucleic acid encoding an acetolactate synthase (ALS) protein that provides resistance to ALS inhibitors, e.g., sulphonylurea and imidazolinone compounds, is provided. The nucleic acid may be used as a selectable marker for expression of a protein of interest in host cells.Type: GrantFiled: September 21, 2007Date of Patent: March 20, 2012Assignee: Danisco US Inc.Inventors: Benjamin S. Bower, Nigel Dunn-Coleman, Nicholas Leiva
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Patent number: 8137961Abstract: The present invention provides nucleic acid molecules and sequences, particularly those identified and obtained from plants, that are useful for transferring and integrating one polynucleotide into another via plant transformation techniques.Type: GrantFiled: March 7, 2006Date of Patent: March 20, 2012Assignee: J.R. Simplot CompanyInventor: Caius Rommens
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Publication number: 20120064101Abstract: The present invention relates to compounds and methods useful for the detection and treatment of disorders associated with frameshift mutations in coding microsatellite regions. The compounds and methods are applicable in cancers, especially of DNA mismatch repair deficient (MMR) sporadic tumours and HNPCC associated tumours. The compounds are useful for detection of disorders and in therapy such as immuno-therapy. The diagnostic methods relate to diagnosis and prognostic assessment of disorders associated with frameshift polypeptides originating from frameshift mutations in coding microsatellite regions of genes based on the detection of immunological entities directed against said frameshift polypeptides in body fluids.Type: ApplicationFiled: October 28, 2011Publication date: March 15, 2012Inventors: Magnus Von Knebel-Doeberitz, Johannes Gebert, Michael Linnebacher, Stefan Woerner, Ruediger Ridder, Peer Bork, Yan Ping Yuan
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Publication number: 20120066781Abstract: This invention relates to phytases, polynucleotides encoding them, uses of the polynucleotides and polypeptides of the invention, as well as the production and isolation of such polynucleotides and polypeptides. In particular, the invention provides polypeptides having phytase activity under high temperature conditions, and phytases that retain activity after exposure to high temperatures. The invention further provides phytases which have increased gastric lability. The phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be formulated as foods or feeds or supplements for either to, e.g., aid in the digestion of phytate.Type: ApplicationFiled: May 20, 2010Publication date: March 15, 2012Inventors: David P. Weiner, Arne I. Solbak, JR., Ryan McCann
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Publication number: 20120064541Abstract: The present invention relates to new galactosyltransferases, nucleic acids encoding them, as well as recombinant vectors, host cells, antibodies, uses and methods relating thereto.Type: ApplicationFiled: May 28, 2010Publication date: March 15, 2012Applicants: ETH ZÜRICH, UNIVERSITÄT FÜR BODENKULTUR WIEN, UNIVERSITÄT ZÜRICHInventors: Markus Künzler, Markus Aebi, Lain Wilson, Alexander Walter Titz, Michael Hengartner, Alex Butschi
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Publication number: 20120064194Abstract: The present invention relates to enzymes and processes. In particular, there is described a host 3140 cell transformed or transfected with a nucleic acid encoding a bacterial phytase enzyme and variants thereof.Type: ApplicationFiled: July 27, 2011Publication date: March 15, 2012Inventors: Andrei Miasnikov, Vijay Kumar, Oliver Kensch, Klaus Pellengahr, Birgitta Leuthner, Ulrich Kettling, Andre Koltermann
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Publication number: 20120065100Abstract: The present invention relates to phosphodiesterase 9A (PDE9A) for use as a marker for prostate cancer, and the use of PDE9A as a marker for diagnosing, detecting, monitoring or prognosticating prostate cancer or the progression of prostate cancer. The present invention also relates to a composition for diagnosing, detecting, monitoring or prognosticating prostate cancer or the progression of prostate cancer, a corresponding method and immunoassay, a method for diagnosing, monitoring or prognosticating hormone-resistant prostate cancer vs. hormone-sensitive prostate cancer, a corresponding immunoassay, a method of data acquisition, an immunoassay for diagnosing, detecting, monitoring or prognosticating prostate cancer or the progression of prostate cancer, a method of identifying an individual for eligibility for prostate cancer therapy, an immunoassay for stratifying an individual or cohort of individuals with a prostate cancer disease, an immunoassay for stratifying an individual with prostate cancer.Type: ApplicationFiled: May 11, 2010Publication date: March 15, 2012Applicants: THE UNIVERSITY COURT OF THE UNIVERSITY OF GLASGOW, KONINKLIJKE PHILIPS ELECTRONICS N.V.Inventors: Ralf Hoffmann, Miles Douglas Houslay, David James Peter Henderson
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Publication number: 20120064059Abstract: A single chain, polypeptide fusion protein, comprising: a non-cytotoxic protease, or a fragment thereof, which protease or protease fragment is capable of cleaving a protein of the exocytic fusion apparatus of a nociceptive sensory afferent; a Targeting Moiety that is capable of binding to a Binding Site on the nociceptive sensory afferent, which Binding Site is capable of undergoing endocytosis to be incorporated into an endosome within the nociceptive sensory afferent; a protease cleavage site at which site the fusion protein is cleavable by a protease, wherein the protease cleavage site is located between the non-cytotoxic protease or fragment thereof and the Targeting Moiety; a translocation domain that is capable of translocating the protease or protease fragment from within an endosome, across the endosomal membrane and into the cytosol of the nociceptive sensory afferent. Nucleic acids encoding the fusion proteins, methods of preparing same and uses thereof are also described.Type: ApplicationFiled: September 22, 2011Publication date: March 15, 2012Applicants: ALLERGAN, INC., SYNTAXIN LTD.Inventors: Keith FOSTER, John CHADDOCK, Philip MARKS, Patrick STANCOMBE, Kei Roger AOKI, Joseph FRANCIS, Lance STEWARD
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Publication number: 20120064112Abstract: The present invention concerns an antigenomic RNA of Newcastle Disease virus (NDV) carrying one or more foreign genes inserted before NP gene, between P and M genes, and/or between HN and L genes. The invention is also directed toward a cDNA encoding a recombinant antigenomic RNA having one or more foreign genes inserted according to the invention, a cell containing the cDNA, a plasmid comprising the cDNA, a cell containing the plasmid, a cell containing the recombinant antigenomic RNA, and a recombinant NDV containing the recombinant antigenomic RNA of the invention, such as a recombinant NDV carrying one or more foreign genes recovered from transcription of the cDNA or the plasmid in a competent cell. The recombinant NDV carrying the one or more foreign genes can be used as a vaccine or vaccine vector.Type: ApplicationFiled: July 21, 2011Publication date: March 15, 2012Applicant: THE UNIVERSITY OF MARYLAND, COLLEGE PARKInventors: SIBA K. SAMAL, ZHUHUI HUANG
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Publication number: 20120064586Abstract: The present invention concerns multimeric oxidoreductase complexes which function in the enzymatic conversion of a carbon substrate, said complexes having a dehydrogenase subunit and a cytochrome C subunit. The invention further relates to polynucleotides coding for the multimeric complexes and methods of use thereof.Type: ApplicationFiled: September 9, 2011Publication date: March 15, 2012Inventors: Robert M. Caldwell, M. Harunur Rashid, Fernando Valle
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Publication number: 20120064620Abstract: The present invention refers to methods for selectively recognizing a base pair in a DNA sequence by a polypeptide, to modified polypeptides which specifically recognize one or more base pairs in a DNA sequence and, to DNA which is modified so that it can be specifically recognized by a polypeptide and to uses of the polypeptide and DNA in specific DNA targeting as well as to methods of modulating expression of target genes in a cell.Type: ApplicationFiled: January 28, 2011Publication date: March 15, 2012Inventors: Ulla Bonas, Jens Boch, Sebastian Schornack, Thomas Lahaye
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Publication number: 20120064577Abstract: Provided are mutant polymerases that comprise a deletion of at least four amino acids among the amino acids at positions corresponding to 167-174 of SEQ ID NO:1. Also provided are mutant polymerases having greater resistance to 30 mM NaCl, 7.5 mM phosphate, or 20 ?g/ml single stranded DNA than a wild-type T7 RNA polymerase having SEQ ID NO:1 or a wild-type T3 RNA polymerase having SEQ ID NO:3. Nucleic acids comprising a nucleotide sequence encoding any of the above mutant polymerases are also provided, as are vectors comprising those nucleic acids and host cells transformed with the vectors Additionally, methods of amplifying mRNA using the mutant polymerases described herein are also provided. Further, compositions comprising any of the mutant polymerases described herein, and a reagent at a concentration that is inhibitory to wild-type T7 RNA polymerase is provided.Type: ApplicationFiled: September 13, 2010Publication date: March 15, 2012Applicant: ENZO BIOCHEM, INC.Inventor: Jack Coleman
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Publication number: 20120065106Abstract: Methods for enhancing expression levels, secretion, and purification of heterologous fusion proteins in a host cell are disclosed.Type: ApplicationFiled: December 28, 2007Publication date: March 15, 2012Applicant: LIFESENSORS, INC.Inventors: Tauseef R. Butt, Tadas Panavas, Amolkumar Karwa, Raymond J. Peroutka, Jeffrey G. Marblestone
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Publication number: 20120064591Abstract: The present invention provides isolated Elo1 and Mig3 nucleic acid sequences capable of conferring increased ethanol tolerance on recombinant yeast and methods of using same in biofuel production, particularly ethanol production. Methods of bioengineering yeast using the Elo1 and, or, Mig3 nucleic acid sequences are also provided.Type: ApplicationFiled: September 14, 2011Publication date: March 15, 2012Applicant: Wisconsin Alumni Research FoundationInventors: Audrey P. Gasch, Jeffrey A. Lewis
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Publication number: 20120064629Abstract: This disclosure provides methods of designing and generating polypeptide variants that have altered properties compared to a parent polypeptide. The present disclosure provides methods of generating polypeptide variants, for example, variant isoprenoid synthases and/or variant prenyl transferases that have at least one desired property not present in the parent polypeptide. The present disclosure further provides polypeptides and polynucleotides encoding variant polypeptides, as well as vectors and host cells comprising the polynucleotides that encode the variant polypeptides. In other embodiments, the present disclosure provides methods of using the variant polypeptides to generate useful products, such as isoprenoid compounds and/or isoprenoid products.Type: ApplicationFiled: March 29, 2010Publication date: March 15, 2012Applicant: Shapphire Energy, Inc.Inventors: Michael Mendez, Craig A. Behnke, Nicole A. Heaps
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Publication number: 20120065148Abstract: The present invention relates to phosphodiesterase 4D7 (PDE4D7) for use as a marker for prostate cancer, and the use of PDE4D7 as a marker for diagnosing, detecting, monitoring or prognosticating prostate cancer or the progression of prostate cancer. The present invention also relates to a composition for diagnosing, detecting, monitoring or prognosticating prostate cancer or the progression of prostate cancer, a corresponding method and immunoassay, a method for diagnosing, monitoring or prognosticating hormone-resistant prostate cancer vs.Type: ApplicationFiled: May 11, 2010Publication date: March 15, 2012Applicants: THE UNIVERSITY COURT OF THE UNIVERSITY OF GLASGOW, KONINKLIJKE PHILIPS ELECTRONICS N.V.Inventors: Ralf Hoffmann, Miles Douglas Houslay, David James Peter Henderson
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Publication number: 20120065251Abstract: The invention provides a nucleic acid construct comprising a promoter sequence derived from microRNA-21 (miR-21) linked to a nucleic acid sequence encoding an anti-cancer agent, an example of which is a toxin. The constructs of the invention are particularly useful for treating tumors expressing miR-21.Type: ApplicationFiled: January 19, 2010Publication date: March 15, 2012Inventors: Rina Rosin-Arbesfeld, Ella Sklan, Alona Zilberberg, Naama David