Bacillus Thuringiensis Insect Toxin Patents (Class 536/23.71)
  • Patent number: 11193139
    Abstract: This disclosure relates to the field of molecular biology. Provided are novel genes that encode pesticidal proteins. These pesticidal proteins and the nucleic acid sequences that encode them are useful in preparing pesticidal formulations and in the production of transgenic pest-resistant plants. Methods to create or alter pesticidal proteins are provided for altered or enhanced pesticidal activity.
    Type: Grant
    Filed: December 18, 2019
    Date of Patent: December 7, 2021
    Assignees: Chinese Academy of Agricultural Sciences, Pioneer Hi-Bred International, Inc.
    Inventors: Jennifer Kara Barry, Thomas Chad Wolfe, Gusui Wu, Jian-Zhou Zhao, Yueming Shan, Changlong Shu, Jie Zhang
  • Patent number: 11046973
    Abstract: Compositions having pesticidal activity and methods for their use are provided. Compositions include isolated and recombinant polypeptide sequences having pesticidal activity, recombinant and synthetic nucleic acid molecules encoding the pesticidal polypeptides, DNA constructs comprising the nucleic acid molecules, vectors comprising the nucleic acid molecules, host cells comprising the vectors, and antibodies to the pesticidal polypeptides. Nucleotide sequences encoding the polypeptides provided herein can be used in DNA constructs or expression cassettes for transformation and expression in organisms of interest, including microorganisms and plants. The compositions and methods provided herein are useful for the production of organisms with enhanced pest resistance or tolerance. Transgenic plants and seeds comprising a nucleotide sequence that encodes a pesticidal protein of the invention are also provided. Such plants are resistant to insects and other pests.
    Type: Grant
    Filed: April 9, 2018
    Date of Patent: June 29, 2021
    Assignee: AgBiome, Inc.
    Inventors: Jessica Parks, Kira Bulazel Roberts, Rebecca E. Thayer
  • Patent number: 10227599
    Abstract: The present invention provides regulatory polynucleotide molecules isolated from plant proline rich protein genes and linked to a viral enhancer molecule. The invention further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds containing the regulatory polynucleotide sequences, and methods for preparing and using the same.
    Type: Grant
    Filed: July 28, 2016
    Date of Patent: March 12, 2019
    Assignee: Monsanto Technology LLC
    Inventors: Stanislaw Flasinski, Shirley Xiaoli Guo, Wei Wu
  • Patent number: 10047366
    Abstract: The present invention relates to the field of chromosome engineering. More specifically, the present invention provides methods and compositions useful for inducibly linearizing circular DNA molecules in vivo in yeast. In one embodiment, a comprises a nucleic acid encoding a selectable marker, wherein the nucleic acid encoding a selectable marker comprises an intron comprising an endonuclease recognition site flanked by telomere seed sequences.
    Type: Grant
    Filed: March 6, 2014
    Date of Patent: August 14, 2018
    Assignee: The Johns Hopkins University
    Inventors: Jef D. Boeke, Leslie Mitchell
  • Patent number: 10028510
    Abstract: DIG-17 insecticidal toxins, polynucleotides encoding such toxins, use of such toxins to control pests, and transgenic plants that produce such toxins are disclosed.
    Type: Grant
    Filed: August 20, 2015
    Date of Patent: July 24, 2018
    Assignee: Dow AgroSciences LLC
    Inventors: Justin M. Lira, Holly Jean Butler, Timothy D. Hey, Doug A. Smith, Kenneth Narva, Aaron T. Woosley
  • Patent number: 9487798
    Abstract: DIG-10 Cry toxins, polynucleotides encoding such toxins, use of such toxins to control pests, and transgenic plants that produce such toxins are disclosed.
    Type: Grant
    Filed: May 17, 2013
    Date of Patent: November 8, 2016
    Assignee: Dow AgroSciences LLC
    Inventors: Justin M. Lira, Kenneth Narva, Aaron T. Woosley, Ignacio M. Larrinua, Timothy D. Hey
  • Patent number: 9447153
    Abstract: The present invention includes domain I modifications that improve various attributes of various Coleopteran-active Cry proteins. These attributes can include improved target pest spectrum, potency, and insect resistance management. The subject modifications can affect protoxin activation and the efficiency of pore formation, which can lead to enhanced insect intoxication.
    Type: Grant
    Filed: September 17, 2012
    Date of Patent: September 20, 2016
    Assignee: Dow AgroSciences LLC
    Inventors: Timothy D. Hey, Ignacio M. Larrinua, Kenneth E. Narva
  • Patent number: 9320233
    Abstract: The invention relates to the soybean variety designated XB55B13. Provided by the invention are the seeds, plants and derivatives of the soybean variety XB55B13. Also provided by the invention are tissue cultures of the soybean variety XB55B13 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety XB55B13 with itself or another soybean variety and plants produced by such methods.
    Type: Grant
    Filed: August 28, 2014
    Date of Patent: April 26, 2016
    Assignee: Monsanto Technology LLC
    Inventor: Jesse Gilsinger
  • Patent number: 9322033
    Abstract: The present invention discloses Hemipteran insect inhibitory proteins, methods of using such proteins, nucleotide sequences encoding such proteins, methods of detecting and isolating such proteins, and their use in agricultural systems.
    Type: Grant
    Filed: April 5, 2013
    Date of Patent: April 26, 2016
    Assignee: Monsanto Technology LLC
    Inventors: James A. Baum, Artem G. Evdokimov, Farhad Moshiri, Timothy J. Rydel, Eric J. Sturman, Moritz von Rechenberg, Halong Vu, Andrew M. Wollacott, Meiying Zheng
  • Patent number: 9234208
    Abstract: DIG-13 insecticidal toxins, polynucleotides encoding such toxins, use of such toxins to control pests, and transgenic plants that produce such toxins are disclosed.
    Type: Grant
    Filed: April 11, 2011
    Date of Patent: January 12, 2016
    Assignee: Dow AgroSciences LLC
    Inventors: Justin M. Lira, Holly J. Butler, Doug A. Smith, Kenneth Narva, Aaron T. Woosley
  • Patent number: 9006520
    Abstract: DIG-3 Cry toxins, polynucleotides encoding such toxins, and transgenic plants that produce such toxins are useful to control insect pests.
    Type: Grant
    Filed: November 5, 2012
    Date of Patent: April 14, 2015
    Assignee: Dow AgroSciences LLC
    Inventors: Justin M. Lira, Holly Jean Butler, Doug A. Smith, Kenneth Narva, Thomas Meade
  • Patent number: 9000261
    Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
    Type: Grant
    Filed: January 23, 2012
    Date of Patent: April 7, 2015
    Assignee: Pioneer Hi Bred International Inc
    Inventors: Andre R. Abad, Hua Dong, Sue B. Lo, Xiaomei Shi, Thomas C. Wolfe
  • Publication number: 20150047076
    Abstract: The present invention provides novel insecticidal proteins active against a Coleopteran and/or Hemipteran species pest, which include, but are not limited to, TIC3131, TIC3400 and TIC3407 proteins. The present invention also provides a DNA construct comprising operably linked to a heterologous promoter a polynucleotide that encodes the novel insecticidal protein or an insecticidal fragment thereof. The present invention further provides transgenic plants/plant cells/plant parts expressing the insecticidal proteins, methods for detecting the presence of the polynucleotide or the protein in a biological sample, and methods of controlling a Coleopteran and/or Hemipteran species pest using the insecticidal proteins.
    Type: Application
    Filed: August 8, 2014
    Publication date: February 12, 2015
    Applicant: Monsanto Technology LLC
    Inventors: Heather M. Anderson, David J. Bowen, Catherine A. Chay, Stanislaw Flasinski, Uma R. Kesanapalli, Jason S. Milligan, Rachael N. Slightom, Yong Yin
  • Publication number: 20150020236
    Abstract: Engineered pesticidal polypeptides that are highly active against a wide range of pests and methods of making such polypeptides are disclosed. The nucleotide sequences encoding the pesticidal polypeptide can be used to transform various prokaryotic and eukaryotic organisms, which organisms can be used to produce the pesticidal polypeptides. The recombinant organisms and/or the polypeptides produced by the recombinant organisms can be used to control pests in various environments.
    Type: Application
    Filed: January 23, 2013
    Publication date: January 15, 2015
    Applicant: SYNGENTA PARTICIPATIONS AG
    Inventors: Mikyong Lee, Jeng Shong Chen, Cheryl Marie De Fontes, Jared Conville, Narendra Palekar
  • Patent number: 8933299
    Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Coleoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
    Type: Grant
    Filed: August 17, 2011
    Date of Patent: January 13, 2015
    Assignee: Pioneer Hi Bred International Inc
    Inventors: Andre R. Abad, Hua Dong, Sue B. Lo, Xiaomei Shi
  • Publication number: 20140380521
    Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2 or 3, or the nucleotide sequence set forth in SEQ ID NO:1, as well as variants and fragments thereof.
    Type: Application
    Filed: June 29, 2012
    Publication date: December 25, 2014
    Applicant: Athenix Corp.
    Inventors: Candace Poutre, Theodore Kahn, Alissa Anthony
  • Publication number: 20140373195
    Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, or the nucleotide sequence set forth in SEQ ID NO: 1 or 3, as well as variants and fragments thereof.
    Type: Application
    Filed: March 7, 2013
    Publication date: December 18, 2014
    Applicant: Athenix Corp.
    Inventors: Kimberly Sampson, Rebecca Thayer, Duane Lehtinen
  • Publication number: 20140352001
    Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
    Type: Application
    Filed: August 11, 2014
    Publication date: November 27, 2014
    Inventors: Andre Roger Abad, Hua Dong, Sue Lo, Xiaomei Shi, Thomas Chad Wolfe
  • Publication number: 20140352000
    Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
    Type: Application
    Filed: August 11, 2014
    Publication date: November 27, 2014
    Inventors: Andre Roger Abad, Hua Dong, Sue Lo, Xiaomei Shi, Thomas Chad Wolfe
  • Publication number: 20140344999
    Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed.
    Type: Application
    Filed: August 5, 2014
    Publication date: November 20, 2014
    Applicant: ATHENIX CORPORATION
    Inventors: Nadine Carozzi, Michael G. Koziel, Nicholas Duck, Nalini M. Desai, Rong Guo, Daniel John Tomso, Rebekah Deter, Tracy Hargiss
  • Patent number: 8878007
    Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
    Type: Grant
    Filed: March 7, 2012
    Date of Patent: November 4, 2014
    Assignee: Pioneer Hi Bred International Inc
    Inventors: Andre R. Abad, Hua Dong, Sue B. Lo, Xiaomei Shi, Thomas C. Wolfe
  • Patent number: 8829279
    Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed.
    Type: Grant
    Filed: June 24, 2011
    Date of Patent: September 9, 2014
    Assignee: Athenix Corporation
    Inventors: Nadine Carozzi, Michael G. Koziel, Nicholas Duck, Nalini M. Desai, Rong Guo, Daniel John Tomso, Rebekah Deter, Tracy Hargiss
  • Patent number: 8822789
    Abstract: An inbred corn line, designated BB86, is disclosed. The invention relates to the seeds of inbred corn line BB86, to the plants and plant parts of inbred corn line BB86 and to methods for producing a corn plant, either inbred or hybrid, by crossing inbred corn line BB86 with itself or another corn line. The invention also relates to products produced from the seeds, plants, or parts thereof, of inbred corn line BB86 and/or of the hybrids produced using the inbred as a parent. The invention further relates to methods for producing a corn plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other inbred corn lines derived from inbred corn line BB86.
    Type: Grant
    Filed: March 9, 2012
    Date of Patent: September 2, 2014
    Assignees: Limagrain Europe S.A., KWS SAAT AG
    Inventor: Freeman C. Whitehead
  • Patent number: 8822762
    Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
    Type: Grant
    Filed: December 22, 2011
    Date of Patent: September 2, 2014
    Assignee: Pioneer Hi Bred International Inc
    Inventors: Lu Liu, Takashi Yamamoto, Yi Zheng
  • Publication number: 20140245491
    Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:13-30, or the nucleotide sequence set forth in SEQ ID NO:1-12, as well as variants and fragments thereof.
    Type: Application
    Filed: May 8, 2014
    Publication date: August 28, 2014
    Applicant: Athenix Corp.
    Inventors: Kimberly Sampson, Daniel Tomso
  • Patent number: 8816157
    Abstract: This invention provides modified, insecticidal Cry34 proteins with enhanced properties as compared to wild-type Cry34 proteins. The modifications to these proteins were based in part on an analysis of the three-dimensional (3D) structure of this protein and other proteins in the Cry34 class. The subject invention also includes polynucleotides that encode these modified proteins, and transgenic plants that produce these modified proteins. This invention further provides methods of controlling plant pests, including rootworms, with these modified proteins. The modified proteins of the subject invention include chimeric toxins involving exchanged segments, domains, and motifs as discussed herein. The subject invention also provides methods of modifying Cry34 proteins.
    Type: Grant
    Filed: February 8, 2013
    Date of Patent: August 26, 2014
    Assignee: Dow AgroSciences, LLC.
    Inventor: H. Ernest Schnepf
  • Patent number: 8809653
    Abstract: An inbred corn line, designated BC110, is disclosed. The invention relates to the seeds of inbred corn line BC110, to the plants and plant parts of inbred corn line BC110 and to methods for producing a corn plant, either inbred or hybrid, by crossing inbred corn line BC110 with itself or another corn line. The invention also relates to products produced from the seeds, plants, or parts thereof, of inbred corn line BC110 and/or of the hybrids produced using the inbred as a parent. The invention further relates to methods for producing a corn plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other inbred corn lines derived from inbred corn line BC110.
    Type: Grant
    Filed: March 9, 2012
    Date of Patent: August 19, 2014
    Assignees: Limagrain Europe S.A., KWS SAAT AG
    Inventor: Thomas C. Koch
  • Patent number: 8809651
    Abstract: An inbred corn line, designated BB93, is disclosed. The invention relates to the seeds of inbred corn line BB93, to the plants and plant parts of inbred corn line BB93 and to methods for producing a corn plant, either inbred or hybrid, by crossing inbred corn line BB93 with itself or another corn line. The invention also relates to products produced from the seeds, plants, or parts thereof, of inbred corn line BB93 and/or of the hybrids produced using the inbred as a parent. The invention further relates to methods for producing a corn plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other inbred corn lines derived from inbred corn line BB93.
    Type: Grant
    Filed: March 9, 2012
    Date of Patent: August 19, 2014
    Assignees: Limagrain Europe S.A., KWS Saat AG
    Inventor: Antoon Van Der Reijden
  • Patent number: 8809652
    Abstract: An inbred corn line, designated LM3, is disclosed. The invention relates to the seeds of inbred corn line LM3, to the plants and plant parts of inbred corn line LM3 and to methods for producing a corn plant, either inbred or hybrid, by crossing inbred corn line LM3 with itself or another corn line. The invention also relates to products produced from the seeds, plants, or parts thereof, of inbred corn line LM3 and/or of the hybrids produced using the inbred as a parent. The invention further relates to methods for producing a corn plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other inbred corn lines derived from inbred corn line LM3.
    Type: Grant
    Filed: March 9, 2012
    Date of Patent: August 19, 2014
    Assignees: Limagrain Europe S.A., KWS SAAT AG
    Inventor: Antoon Van Der Reijden
  • Patent number: 8802945
    Abstract: An inbred corn line, designated CB15, is disclosed. The invention relates to the seeds of inbred corn line CB15, to the plants and plant parts of inbred corn line CB15 and to methods for producing a corn plant, either inbred or hybrid, by crossing inbred corn line CB15 with itself or another corn line. The invention also relates to products produced from the seeds, plants, or parts thereof, of inbred corn line CB15 and/or of the hybrids produced using the inbred as a parent. The invention further relates to methods for producing a corn plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other inbred corn lines derived from inbred corn line CB15.
    Type: Grant
    Filed: March 9, 2012
    Date of Patent: August 12, 2014
    Assignees: Limagrain Europe S.A., KWS SAAT AG
    Inventor: Freeman C. Whitehead
  • Publication number: 20140223598
    Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:3-5, or the nucleotide sequence set forth in SEQ ID NO:2, as well as variants and fragments thereof.
    Type: Application
    Filed: July 27, 2012
    Publication date: August 7, 2014
    Applicant: Athenix Corp.
    Inventors: Kimberly S. Sampson, Duane A. Lehtinen
  • Patent number: 8796026
    Abstract: The present invention relates to the isolation and characterization of nucleotide sequences encoding novel insecticidal proteins secreted into the extracellular space from Bacillus thuringiensis and related strains. The proteins are isolated from culture supernatants of Bacillus thuringiensis and related strains and display insecticidal activity against coleopteran insects including Colorado potato beetle (Lymantria dispar) and Southern Corn Rootworm (Diabrotica undecempunctata). Insecticidal proteins encoded by nucleotide sequences that hybridize to the isolated and characterized nucleotide sequences are disclosed. Also disclosed are methods of making and using transgenic cells and plants comprising the novel nucleotide sequence of the invention.
    Type: Grant
    Filed: July 6, 2004
    Date of Patent: August 5, 2014
    Assignee: Monsanto Technology LLC
    Inventors: James A. Baum, Judith C. Donovan, William P. Donovan, James T. Engleman, Karina Krasomil-Osterfeld, John W. Pitkin, James K. Roberts
  • Patent number: 8791326
    Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions including a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules having nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, the nucleotide sequence set forth in SEQ ID NO:1, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12, as well as variants and fragments thereof.
    Type: Grant
    Filed: November 22, 2011
    Date of Patent: July 29, 2014
    Assignee: Athenix Corp.
    Inventors: Kimberly S. Sampson, Daniel J. Tomso, Volker Heinrichs
  • Publication number: 20140196175
    Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:21-32, or the nucleotide sequence set forth in SEQ ID NO: 1-20, as well as variants and fragments thereof.
    Type: Application
    Filed: March 13, 2014
    Publication date: July 10, 2014
    Applicant: Athenix Corp.
    Inventors: Kimberly Sampson, Daniel Tomso
  • Patent number: 8772577
    Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
    Type: Grant
    Filed: November 10, 2010
    Date of Patent: July 8, 2014
    Assignee: Pioneer Hi Bred International Inc
    Inventors: Andre R Abad, Hua Dong, Sue B Lo, Xiaomei Shi, Thomas Chad Wolfe
  • Patent number: 8759611
    Abstract: The present invention relates to control of pest infestation by inhibiting one or more biological functions. The invention provides methods and compositions for such control, By feeding one or more recombinant double stranded RNA molecules provided by the invention to the pest, a reduction in pest infestation is obtained through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.
    Type: Grant
    Filed: December 20, 2010
    Date of Patent: June 24, 2014
    Assignee: Monsanto Technology LLC
    Inventors: James A. Buam, Claire A. Cajacob, Pascale Feldmann, Gregory R. Heck, Irene Nooren, Geert Plaetinck, Wendy Maddelein, Ty T. Vaughn
  • Patent number: 8759619
    Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:13-30, or the nucleotide sequence set forth in SEQ ID NO:1-12, as well as variants and fragments thereof.
    Type: Grant
    Filed: February 18, 2011
    Date of Patent: June 24, 2014
    Assignee: Athenix Corp.
    Inventors: Kimberly Sampson, Daniel Tomso
  • Patent number: 8735560
    Abstract: Novel synthetic Bacillus thuringiensis crystal proteins exhibiting insect inhibitory activity is disclosed. Polynucleotides encoding the crystal protein, transgenic plants and microorganisms that contain the polynucleotides, isolated peptides derived from the crystal protein, and antibodies directed against the crystal protein are also provided. Methods of using the crystal protein and polynucleotides encoding the crystal protein to control Lepidopteran insects are also disclosed.
    Type: Grant
    Filed: March 2, 2011
    Date of Patent: May 27, 2014
    Assignee: Monsanto Technology LLC
    Inventors: Leigh H. English, Deborah G. Rucker, Victor M. Guzov
  • Patent number: 8729336
    Abstract: Embodiments of the present invention relate to insecticidal Bacillus thuringiensis Cry1 and Cry2 polypeptides. Methods for using the polypeptides and nucleic acids of embodiments of the invention to synergistically enhance resistance of plants to insect predation are encompassed in embodiments of the present invention.
    Type: Grant
    Filed: January 22, 2010
    Date of Patent: May 20, 2014
    Assignees: Pioneer Hi-Bred International, Inc, E.I. du Pont de Nemours and Company
    Inventors: John L. Flexner, Deirdre M. Kapka-Kitzman, Lisa Procyk, Bruce Stanley, Jianzhou Zhao
  • Patent number: 8697642
    Abstract: DIG-10 Cry toxins, polynucleotides encoding such toxins, use of such toxins to control pests, and transgenic plants that produce such toxins are disclosed.
    Type: Grant
    Filed: June 14, 2010
    Date of Patent: April 15, 2014
    Assignee: Dow AgroSciences, LLC.
    Inventors: Justin M. Lira, Kenneth Narva, Aaron T. Woosley, Ignacio M. Larrinua, Timothy D. Hey
  • Patent number: 8692066
    Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Coleoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
    Type: Grant
    Filed: December 8, 2010
    Date of Patent: April 8, 2014
    Assignee: Pioneer Hi Bred International Inc
    Inventors: Andre R Abad, Hua Dong, Sue B Lo, Xiaomei Shi
  • Patent number: 8692065
    Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
    Type: Grant
    Filed: December 1, 2009
    Date of Patent: April 8, 2014
    Assignee: Pioneer Hi Bred International Inc
    Inventors: Andre R. Abad, Deirdre M. Kapka-Kitzman, John P. Mathis, Thomas C. Wolfe
  • Publication number: 20140096281
    Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:4-19, or the nucleotide sequence set forth in SEQ ID NO: 1-3, as well as variants and fragments thereof.
    Type: Application
    Filed: March 29, 2012
    Publication date: April 3, 2014
    Applicant: Althenix Corp.
    Inventor: Kimberly S. Sampson
  • Patent number: 8686232
    Abstract: Nucleic acid molecules encoding novel Vip3 toxins that are highly active against a wide range of lepidopteran insect pests are disclosed. The nucleic acid molecules can be used to transform various prokaryotic and eukaryotic organisms to express the Vip3 toxins. These recombinant organisms can be used to control lepidopteran insects in various environments.
    Type: Grant
    Filed: April 21, 2008
    Date of Patent: April 1, 2014
    Assignee: Syngenta Participations AG
    Inventors: Zhicheng Shen, Gregory W. Warren, Vance Kramer, Frank Shotkoski
  • Patent number: 8686124
    Abstract: Compositions and methods for conferring pesticidal activity, including against lepidopteran pests, to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:27 and 28, or the nucleotide sequence set forth in SEQ ID NO:3, 8, 13, and 18, as well as variants and fragments thereof.
    Type: Grant
    Filed: February 18, 2011
    Date of Patent: April 1, 2014
    Assignee: Athenix Corp.
    Inventors: Kimberly Sampson, Daniel Tomso
  • Patent number: 8686233
    Abstract: The present invention provides insecticidal polypeptides related to shuffled Bacillus thuringiensis Cry1 polypeptides. Nucleic acids encoding the polypeptides of the invention are also provided. Methods for using the polypeptides and nucleic acids of the invention to enhance resistance of plants to insect predation are encompassed.
    Type: Grant
    Filed: January 24, 2012
    Date of Patent: April 1, 2014
    Assignee: Pioneer Hi Bred International Inc
    Inventors: David Cerf, Ruth Cong, Michael Freeman, Kevin McBride, Takashi Yamamoto
  • Publication number: 20140073582
    Abstract: The invention relates to nucleic acid molecules encoding insecticidal proteins of the Cry family, derived from Bacillus thuringiensis, exhibiting cytotoxic activity against cancer and/or tumor cells of humans and/or animals, but not against normal cells. The invention also provides proteins and compositions of proteins of the Cry family, derived from the bacteria Bacillus thuringiensis, that exhibit cytotoxic activity preferably against cancer cells of humans and/or animals, without affecting the normal cells. The Cry proteins of the invention do not exhibit any hemolytic activity, nor do they belong to the parasporin group. The invention also relates to methods for treating cancer and/or tumor cells of humans and/or animals, and for preventing metastasis, using insecticidal Cry proteins of Bacillus thuringiensis.
    Type: Application
    Filed: December 16, 2011
    Publication date: March 13, 2014
    Applicant: Center for Scientific Research and Higher Education at Ensenda, Baja California
    Inventors: Jorge Olmos Soto, Efraín Arias Bañuelos, Gretel Mendoza Almanza
  • Publication number: 20140037608
    Abstract: The invention relates to an insecticidal protein, its gene encoding and the uses thereof. The protein comprises: (a) a protein consisting of an amino acid sequence shown by SEQ ID NO:2; or (b) a protein derived from (a) consisting of an amino acid sequence by substitution, deletion, or addition of one or more amino acid residues of the amino acid sequences in (a), and having insecticidal activity; or (c) a protein generated by the expression of nucleic acid molecules containing a nucleotide sequence of SEQ ID NO:1; or (d) a protein generated by the expression of nucleic acid molecules containing a complementary sequence that hybridized with SEQ ID NO:1 under stringent conditions; or (e) a protein generated by the expression of nucleic acid molecules that contain nucleotide sequences isocoding with the nucleotide sequences in (d). The insecticidal protein of the invention has high expression level and strong toxicity against pests.
    Type: Application
    Filed: March 18, 2013
    Publication date: February 6, 2014
    Inventors: AIHONG ZHANG, Jie Pang, Ruiqi Niu, Lihong Niu, Na Wang, Jincun Huang, Yujie Qi
  • Publication number: 20140037609
    Abstract: The invention relates to an insecticidal protein, its gene encoding and the uses thereof. The protein comprises: (a) a protein consisting of an amino acid sequence shown by SEQ ID NO:2 or a sequence of amino acid residuses at positions 1 to 640 thereof; or (b) a protein derived from (a) by substitution, deletion, or addition of one or more amino acid residuses of the amino acid sequences in (a), and having insecticidal activity; or (c) a protein generated by the expression of nucleic acid molecules containing a sequence of amino acid residuses at the positions 1 to 1920 of SEQ ID NO:1; or (d) a protein generated by the expression of nucleic acid molecules containing a complementary sequence that hybridized with the sequence of nucleotides at the positions 1 to 1920 of SEQ ID NO:1 under stringent conditions. The protein has high expression level and strong toxicity against pests.
    Type: Application
    Filed: March 18, 2013
    Publication date: February 6, 2014
    Inventors: AIHONG ZHANG, Jie Pang, Ruiqi Niu, Lihong Niu, Chao Gao, Mengmeng Miao, Donghai Yang
  • Publication number: 20140033363
    Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2-5, or the nucleotide sequence set forth in SEQ ID NO: 1, as well as variants and fragments thereof.
    Type: Application
    Filed: March 29, 2012
    Publication date: January 30, 2014
    Applicant: ATHENIX CORP.
    Inventor: Kimberly Sampson