Bacillus Thuringiensis Insect Toxin Patents (Class 536/23.71)
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Patent number: 7910807Abstract: Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.Type: GrantFiled: November 14, 2007Date of Patent: March 22, 2011Assignees: Pioneer Hi-Bred International, Inc., E.I. DuPont de Nemours and CompanyInventors: Andre R. Abad, Ronald D. Flannagan, Rafael Herrmann, Albert L. Lu, Billy F. McCutchen, James K. Presnail, Janet A. Rice, James F. Wong, Cao-Guo Yu
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Publication number: 20110055968Abstract: The present invention provides insecticidal polypeptides related to shuffled Bacillus thuringiensis Cry1 polypeptides. Nucleic acids encoding the polypeptides of the invention are also provided. Methods for using the polypeptides and nucleic acids of the invention to enhance resistance of plants to insect predation are encompassed.Type: ApplicationFiled: July 23, 2009Publication date: March 3, 2011Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: DAVID CERF, RUTH CONG, MICHAEL FREEMAN, KEVIN E. McBRIDE, TAKASHI YAMAMOTO
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Patent number: 7893228Abstract: Reagents, methods and kits for the purification of RNA from biological materials are provided.Type: GrantFiled: October 12, 2001Date of Patent: February 22, 2011Assignee: Qiagen North American Holdings, Inc.Inventors: Ellen M. Heath, John M. Wages, Jr.
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Publication number: 20110041213Abstract: The invention pertains to novel insecticidal compounds derived from Bacillus thuringiensis strains. New proteins designated. Cry2Ae, Cry2Af, and Cry2Ag, and variants thereof are provided, as well as DNA sequences encoding these proteins or their variants. Further provided are recombinant hosts expressing such proteins, particularly plant cells and plants.Type: ApplicationFiled: September 23, 2010Publication date: February 17, 2011Applicant: Bayer BioScience N.V.Inventors: Greta Arnaut, Annemie Boets, Stijn Vanneste, Jeroen Van Rie, Sara Van Houdt
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Patent number: 7888471Abstract: Four novel Bacillus thuringiensis strains, which are deposited at the BCCM-LMG under accession nos. LMG P-12592, LMG P-12593, LMG P-12594, and LMG P-13493, produce new crystal proteins during sporulation that are toxic to Lepidoptera, more particularly against Noctuidae such as Spodoptera spp. and Agrotis ipsilon, against Pyralidae such as Ostrinta nubilalis, and against Yponomeutidae such as Plutella xylostella, and that are encoded by a novel gene. The crystal proteins contain protoxins, which can yield a toxin as trypsin-digestion product. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains and that encodes its respective toxin, is resistant to Lepidoptera. Each strain, itself, or its crystals, crystal proteins, protoxin or toxin can be used as the active ingredient in an insecticidal composition for combatting Lepidoptera.Type: GrantFiled: March 14, 2008Date of Patent: February 15, 2011Assignee: Bayer Bioscience N.V.Inventors: Bart Lambert, Stefan Jansens, Katrien Van Audenhove, Marnix Peferoen
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Patent number: 7884199Abstract: The present invention is directed to mutants of HCV NS3/4A protease. More particularly, the present invention identifies mutant of HCV NS3/4A protease that are resistant to drug treatment.Type: GrantFiled: November 17, 2008Date of Patent: February 8, 2011Assignee: Vertex Pharmaceuticals IncorporatedInventors: Chao Lin, Kai Lin
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Publication number: 20110030096Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:28-62, or the nucleotide sequence set forth in SEQ ID NO:1-27, as well as variants and fragments thereof.Type: ApplicationFiled: July 30, 2010Publication date: February 3, 2011Applicant: Athenix Corp.Inventors: Kimberly S. Sampson, Daniel John Tomso, Rong Guo
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Patent number: 7858849Abstract: The present invention provides insecticidal polypeptides related to shuffled Bacillus thuringiensis Cry1 polypeptides. Nucleic acids encoding the polypeptides of the invention are also provided. Methods for using the polypeptides and nucleic acids of the invention to enhance resistance of plants to insect predation are encompassed.Type: GrantFiled: December 10, 2007Date of Patent: December 28, 2010Assignee: Pioneer Hi-Bred International, Inc.Inventors: David Cerf, Ruth Cong, Michael Freeman, Kevin McBride, Takashi Yamamoto
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Publication number: 20100319092Abstract: DIG-10 Cry toxins, polynucleotides encoding such toxins, use of such toxins to control pests, and transgenic plants that produce such toxins are disclosed.Type: ApplicationFiled: June 14, 2010Publication date: December 16, 2010Applicant: DOW AGROSCIENCES LLCInventors: Justin M. Lira, Kenneth Narva, Aaron T. Woosley, Ignacio M. Larrinua, Timothy D. Hey
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Publication number: 20100317569Abstract: DIG-5 Cry toxins, polynucleotides encoding such toxins, use of such toxins to control pests, and transgenic plants that produce such toxins are disclosed.Type: ApplicationFiled: June 14, 2010Publication date: December 16, 2010Applicant: DOW AGROSCIENCES LLCInventors: Justin M. Lira, Kenneth Narva, Aaron T. Woosley, Ignacio Mario Larrinua, Timothy D. Hey
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Publication number: 20100319093Abstract: DIG-11 Cry toxins, polynucleotides encoding such toxins, use of such toxins to control pests, and transgenic plants that produce such toxins are disclosed.Type: ApplicationFiled: June 14, 2010Publication date: December 16, 2010Applicant: DOW AGROSCIENCES LLCInventors: Justin M. Lira, Kenneth Narva, Aaron T. Woosley, Ignacio M. Larrinua, Timothy D. Hey
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Patent number: 7838293Abstract: The present invention discloses the isolation, cloning and use of insecticidal crystal proteins from Bacillus thuringiensis. The present invention isolates a novel insecticidal crystal protein gene cry7Ba1 from B. thuringiensis subsp. huazhongensis YBT-978 strain, and said gene encodes a novel insecticidal crystal protein Cry7Ba1, which shows insecticidal activity against Lepidopteran insects. The present invention also discloses the gene sequence of the novel insecticidal crystal protein, uses suitable expression vectors to transform micro-organisms so as to express the product Cry7Ba1 encoded by the gene and make it exert the insecticidal activity of the protein against Lepidopteran pests.Type: GrantFiled: October 17, 2006Date of Patent: November 23, 2010Assignee: Huazhong Agricultural UniversityInventors: Ming Sun, Ziniu Yu, Shouwen Chen, Zhenyu Zhang, Lifang Ruan, Suxia Guo, Jingyuan Dai, Lin Li, Ziduo Liu
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Publication number: 20100272757Abstract: The invention relates to improved methods of producing and recovering B. anthracisprotective antigen (PA), especially modified PA which is protease resistant, and to methods of using of these PAs or nucleic acids encoding these PAs for eliciting an immunogenic response in humans, including responses which provide protection against, or reduce the severity of, B. anthracis bacterial infections and which are useful to prevent and/or treat illnesses caused by B. anthracis, such as inhalation anthrax, cutaneous anthrax and gastrointestinal anthrax.Type: ApplicationFiled: June 15, 2010Publication date: October 28, 2010Inventors: Stephen H. Leppla, Yogendra Singh, Kurt Klimpel, Rachel Schneerson, John B. Robbins
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Publication number: 20100269223Abstract: DIG-3 Cry toxins, polynucleotides encoding such toxins, and transgenic plants that produce such toxins are useful to control insect pests.Type: ApplicationFiled: March 24, 2010Publication date: October 21, 2010Applicant: Dow AgroSciences LLCInventors: Justin M. Lira, Holly Jean Butler, Doug A. Smith, Kenneth Narva, Thomas Meade
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Patent number: 7812129Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.Type: GrantFiled: March 12, 2009Date of Patent: October 12, 2010Assignee: Pioneer Hi-Bred International, Inc.Inventors: Andre R. Abad, Hua Dong, Sue B. Lo, Xiaomei Shi
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Patent number: 7803925Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 15, 17, or 19, or the nucleotide sequence set forth in SEQ ID NO:1, 3, 14, 16, or 18, as well as variants and fragments thereof.Type: GrantFiled: July 1, 2008Date of Patent: September 28, 2010Assignee: Athenix Corp.Inventors: Nadine Carozzi, Michael G. Koziel, Tracy Hargiss, Nicholas B. Duck, Theodore W. Kahn
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Patent number: 7803993Abstract: Compositions and methods for protecting a plant from an insect pest are provided. In particular, novel polynucleotides and the pesticidal polypeptides they encode are provided. Methods of using the novel polynucleotides and pesticidal polypeptides of the invention to protect a plant from an insect pest are further provided. Particular embodiments of the invention provide pesticidal compositions and formulations, DNA constructs, and transformed plants, plant cells, and seeds.Type: GrantFiled: September 12, 2007Date of Patent: September 28, 2010Assignees: Pioneer Hi-Bred International, Inc., E.I. du Pont de Nemours and CompanyInventors: Andre R. Abad, Ronald D. Flannagan, Billy F. McCutchen, Cao Guo Yu
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Patent number: 7790961Abstract: The subject invention concerns new classes of pesticidally active proteins and the polynucleotide sequences that encode these proteins. In preferred embodiments, these pesticidal proteins have molecular weights of approximately 40-50 kDa and of approximately 10-15 kDa.Type: GrantFiled: June 29, 2007Date of Patent: September 7, 2010Assignee: Hycogen CorporationInventors: Kenneth E. Narva, H. Ernest Schnepf, Mark Knuth, Michael R. Pollard, Guy A. Cardineau, George E. Schwab, Tracy Ellis Michaels
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Publication number: 20100222273Abstract: This invention provides modified, insecticidal Cry35 proteins with enhanced properties as compared to wild-type Cry35 proteins. The modifications to these proteins were based in part on analysis of the atomic coordinates and three-dimensional (3D) structure of the ˜45 kDa 149B1 protein and other proteins in the Cry35 class. The subject invention also includes polynucleotides that encode these modified proteins, and transgenic plants that produce these modified proteins. This invention further provides methods of controlling plant pests, including rootworms, with these modified proteins. The modified proteins of the subject invention include chimeric toxins involving exchanged segments, domains, and motifs as discussed herein. The subject invention also provides methods of modifying Cry35 proteins.Type: ApplicationFiled: March 2, 2010Publication date: September 2, 2010Applicant: Dow AgroSciences LLCInventors: H. Ernest Schnepf, Kenneth Edwin Narva, Steven Lee Evans
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Publication number: 20100192256Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.Type: ApplicationFiled: December 1, 2009Publication date: July 29, 2010Inventors: Andre R. Abad, Deirdre M. Kapka-Kitzman, John P. Mathis, Thomas C. Wolfe
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Publication number: 20100190707Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.Type: ApplicationFiled: January 6, 2010Publication date: July 29, 2010Inventors: Andre R. Abad, Hua Dong, Deirdre M. Kapka-Kitzman, Sue B. Lo, Xiaomei Shi
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Patent number: 7763465Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.Type: GrantFiled: March 12, 2009Date of Patent: July 27, 2010Assignee: Pioneer Hi-Bred International, Inc.Inventors: Andre R. Abad, Hua Dong, Sue B. Lo, Xiaomei Shi
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Publication number: 20100186123Abstract: The present invention provides a method to obtain DNA constructs that encode 3-domain Cry toxins (also called Cry toxins, Bt toxins, or ?-endotoxins) lacking helix ?-1. These DNA constructs have been modified to encode proteins that kill insects that are resistant to the corresponding unmodified Cry toxins. The DNA constructs encoding the Modified 3-Domain Cry Toxins and the encoded Modified 3-Domain Cry Toxins are provided together with the molecular vectors and the host cell comprising said constructs and the recombinant methods to produce the Modified 3-Domain Cry Toxins. Additionally, compositions comprising the Modified 3-Domain Cry Toxins are disclosed. The resistance of the insects to unmodified Cry toxins is due to reduced toxin binding to the insects' midgut receptors. The invention further provides methods to overcome resistance in crop pests.Type: ApplicationFiled: June 8, 2007Publication date: July 22, 2010Inventors: Liliana Pardo-Lopez, Bruce Elliot Tabashnik, Mario Soberon-Chavez, Maria Alejandra Bravo-De La Parra
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Patent number: 7745700Abstract: The present invention relates to the field of plant pest control, particularly insect control. Provided are nucleotide sequences from Bacillus thuringiensis encoding insecticidal proteins. Further provided are methods and means for using said nucleotide sequence for controlling plant insect pests. It is emphasized that this abstract is provided to comply with the rules requiring an abstract that will allow a searcher or other reader to quickly ascertain the subject matter of the technical disclosure. It is submitted with the understanding that it will not be used to interpret or limit the scope or meaning of the claims.Type: GrantFiled: August 27, 2007Date of Patent: June 29, 2010Assignee: Bayer Bioscience N.V.Inventors: Greta Arnaut, Annemie Boets, Karel De Rudder, Stijn Vanneste, Jeroen Van Rie
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Patent number: 7737263Abstract: This invention provides modified, insecticidal Cry35 proteins with enhanced properties as compared to wild-type Cry35 proteins. The modifications to these proteins were based in part on analysis of the atomic coordinates and three-dimensional (3D) structure of the ˜45 kDa 149B1 protein and other proteins in the Cry35 class. The subject invention also includes polynucleotides that encode these modified proteins, and transgenic plants that produce these modified proteins. This invention further provides methods of controlling plant pests, including rootworms, with these modified proteins. The modified proteins of the subject invention include chimeric toxins involving exchanged segments, domains, and motifs as discussed herein. The subject invention also provides methods of modifying Cry35 proteins.Type: GrantFiled: November 21, 2007Date of Patent: June 15, 2010Assignee: Dow Agrosciences LLCInventors: H. Ernest Schnepf, Kenneth E. Narva, Steven L. Evans
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Patent number: 7737331Abstract: The present invention provides insecticidal polypeptides related to Bacillus Cry2 polypeptides. Nucleic acids encoding the polypeptides of the invention are also provided. Methods for using the polypeptides and nucleic acids of the invention to enhance resistance of plants to insect predation are encompassed.Type: GrantFiled: February 16, 2007Date of Patent: June 15, 2010Assignee: Pioneer Hi-Bred International, Inc.Inventors: Ericka Bermudez, Robin Emig, Kevin McBride, Takashi Yamamoto
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Patent number: 7696412Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding ?-endotoxins having pesticidal activity against pests of the order Coleoptera. The invention further provides mutagenized nucleic acids that have been modified to encode endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, expression cassettes comprising such nucleic acids, and transformed plants and seeds comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.Type: GrantFiled: April 16, 2003Date of Patent: April 13, 2010Assignee: E.I. du Pont de Nemours and CompanyInventors: Andre R. Abad, Nicholas B. Duck, Xiang Feng, Ronald D. Flannagan, Theodore W. Kahn, Lynne E. Sims
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Patent number: 7692068Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, or the nucleotide sequence set forth in SEQ ID NO:1, as well as variants thereof.Type: GrantFiled: January 25, 2007Date of Patent: April 6, 2010Assignee: Athenix CorporationInventors: Nadine Carozzi, Tracy Hargiss, Michael G. Koziel, Nicholas B. Duck, Brian Carr
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Patent number: 7674959Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NOS:2, 11 and 13, or the nucleotide sequences set forth in SEQ ID NOS:1, 10 and 12, as well as variants and fragments thereof.Type: GrantFiled: April 3, 2006Date of Patent: March 9, 2010Assignee: Athenix CorporationInventors: Nadine Carozzi, Tracy Hargiss, Michael G. Koziel, Nicholas B. Duck
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Publication number: 20100037348Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, or the nucleotide sequence set forth in SEQ ID NO:1, as well as variants thereof.Type: ApplicationFiled: January 25, 2007Publication date: February 11, 2010Applicant: Athenix CorporationInventors: Nadine Carozzi, Tracy Hargiss, Michael G. Koziel, Nicholas B. Duck, Brian Carr
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Patent number: 7655838Abstract: Novel insect inhibitory proteins are disclosed comprising two different components, both of which are required for biological activity. Various methods of linking both components together, so that a single protein provides insect inhibitory activity, are disclosed. Also disclosed are novel Bacillus thuringiensis nucleic acid sequences encoding Coleopteran-inhibitory crystal proteins, designated tIC100 (29-kDa) and tlC101 (14-kDa). Also disclosed are methods of making and using nucleic acid sequences in the development of the transgenic plant cells containing the novel nucleic acid sequences disclosed herein.Type: GrantFiled: April 27, 2007Date of Patent: February 2, 2010Assignee: Monsanto Technology LLCInventors: Victor M. Guzov, Thomas M. Malvar, James K. Roberts, Sakuntala Sivasupramaniam
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Publication number: 20100005543Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:61-121 and 133-141, or the nucleotide sequence set forth in SEQ ID NO:1-60, 124-132, and 142-283, as well as variants and fragments thereof.Type: ApplicationFiled: June 25, 2009Publication date: January 7, 2010Applicant: Athenix CorporationInventors: Kimberly S. Sampson, Daniel John Tomso, Shruti Agarwal, Brian McNulty, Chris Campbell
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Publication number: 20090313721Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.Type: ApplicationFiled: April 3, 2009Publication date: December 17, 2009Inventors: Andre R. Abad, Hua Dong, Sue B. Lo, Xiaomei Shi
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Patent number: 7629504Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding Cry9 ?-endotoxins having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, expression cassettes, and transformed plants comprising a nucleic acid of the invention.Type: GrantFiled: December 21, 2004Date of Patent: December 8, 2009Assignees: Pioneer Hi-Bred International, Inc., E. I. du Pont de Nemours and CompanyInventors: Ronald D. Flannagan, Andre R. Abad
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Patent number: 7622572Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 15, 17, or 19, or the nucleotide sequence set forth in SEQ ID NO:1, 3, 14, 16, or 18, as well as variants and fragments thereof.Type: GrantFiled: May 2, 2006Date of Patent: November 24, 2009Assignee: Athenix CorporationInventors: Nadine Carozzi, Michael G. Koziel, Tracy Hargiss, Nicholas B. Duck
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Patent number: 7615686Abstract: A novel pesticidal toxin isolated from Bacillus thuringiensis that is highly active against a wide range of lepidopteran insect pests is disclosed. The DNA encoding the pesticidal toxin can be used to transform various prokaryotic and eukaryotic organisms to express the pesticidal toxin. These recombinant organisms can be used to control lepidopteran insects in various environments.Type: GrantFiled: May 30, 2007Date of Patent: November 10, 2009Assignee: Syngenta Participations AGInventors: Paul Miles, Vance Kramer, Shen Zhicheng, Gregory Warren, Frank Shotkoski
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Patent number: 7605304Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding ?-endotoxins having pesticidal activity against pests of the order Coleoptera. The invention further provides mutagenized nucleic acids that have been modified to encode endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, expression cassettes comprising such nucleic acids, and transformed plants and seeds comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.Type: GrantFiled: October 23, 2001Date of Patent: October 20, 2009Assignee: E.I. du Pont de Nemours and CompanyInventors: Andre R. Abad, Nicholas B. Duck, Xiang Feng, Ronald D. Flannagan, Theodore W. Kahn, Lynne E. Sims
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Patent number: 7569363Abstract: Methods for making a modified Cry3A toxin are disclosed. Such methods include the insertion of a protease recognition site that is recognized by a gut protease of a target insect, such as corn rootworm, into at least one position of a Cry3A toxin so that a modified Cry3A toxin is thus designed. The coding sequence of the modified Cry3A toxin may be transformed into a host cell and the host cell grown under conditions that allow the host cell to produce the modified Cry3A toxin. The host cell may be a plant cell and the plant may be comprised in a transgenic plant. Thus, the transgenic plant may be used to produce the modified Cry3A toxin.Type: GrantFiled: August 6, 2007Date of Patent: August 4, 2009Assignee: Syngenta Participations AGInventors: Eric Chen, Cheryl Stacy
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Publication number: 20090183277Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Coleoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.Type: ApplicationFiled: December 22, 2008Publication date: July 16, 2009Inventors: Andre R. Abad, Hua Dong, Sue B. Lo, Xiaomei Shi
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Publication number: 20090175974Abstract: Disclosed is a novel cadherin peptide that enhances the toxicity of Cry proteins. A novel insecticide composition comprising an effective amount of cadherin peptide having SEQ. ID. NO:2 and an effective amount of Bacillus thuringiensis Cry protein wherein the cadherin peptide comprises a Cry3Aa toxin binding region from the full-length T.Type: ApplicationFiled: November 18, 2008Publication date: July 9, 2009Inventors: Brenda S. Oppert, Juan Luis Jurat-Fuentes, Jeffrey A. Fabrick, Cristopher Oppert
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Patent number: 7541517Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding Cry9 ?-endotoxins having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, expression cassettes, and transformed plants comprising a nucleic acid of the invention.Type: GrantFiled: June 21, 2006Date of Patent: June 2, 2009Assignees: Pioneer Hi-Bred International, Inc., E.I. du Pont de Nemours and CompanyInventors: Ronald D. Flannagan, André Abad
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Patent number: 7521235Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.Type: GrantFiled: July 20, 2007Date of Patent: April 21, 2009Assignee: Pioneer Hi-Bred International, Inc.Inventors: Andre R. Abad, Hua Dong, Sue B. Lo, Xiaomei Shi
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Patent number: 7521550Abstract: The present application relates to an insect resistant transgenic cotton event designated COT202. The application also relates to polynucleotides which are characteristic of the COT202 event, plants comprising the polynucleotides, and methods of detecting the COT202 event.Type: GrantFiled: November 9, 2004Date of Patent: April 21, 2009Assignee: Syngenta Participations AGInventors: David Vincent Negrotto, Frank Arthur Shotkoski, Wenjin Yu
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Publication number: 20090098164Abstract: The present invention concerns spore display methods. More specifically, the invention concerns display of heterologous molecules, such as peptides and polypeptides, on spores of bacilli, such as, for example, Bacillus thuringiensis (Bt) or Bacillus cereus (BC), using externally exposed spore coat proteins or fragments or variants thereof.Type: ApplicationFiled: August 13, 2008Publication date: April 16, 2009Inventors: Ramesh Bhatt, Lawrence Horowitz, Angeles Estelles
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Publication number: 20090094714Abstract: Disclosed are novel insecticidal polypeptides, and compositions comprising these polypeptides, peptide fragments thereof, and antibodies specific therefor. Also disclosed are vectors, transformed host cells, and transgenic plants that contain nucleic acid segments that encode the disclosed ?-endotoxin polypeptides. Also disclosed are methods of identifying related polypeptides and polynucleotides, methods of making and using transgenic cells comprising these polynucleotide sequences, as well as methods for controlling an insect population, such as Colorado potato beetle, southern corn rootworm and western corn rootworm, and for conferring to a plant resistance to a target insect species.Type: ApplicationFiled: October 23, 2008Publication date: April 9, 2009Inventors: Mark J. Rupar, William P. Donovan, Chih-Rei Chu, Elizabeth Pease, Yuping Tan, Annette C. Slaney, Thomas M. Malvar, James A. Baum
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Patent number: 7510878Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.Type: GrantFiled: July 20, 2007Date of Patent: March 31, 2009Assignee: Pioneer Hi-Bred International, Inc.Inventors: Andre R. Abad, Hua Dong, Sue B. Lo, Xiaomei Shi
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Patent number: 7501559Abstract: Plants made resistant to insects by transforming their nuclear genome with two or more DNA sequences, each encoding a different non-competitively binding B. thuringiensis protoxin or insecticidal part thereof, preferably the toxin thereof.Type: GrantFiled: November 9, 2006Date of Patent: March 10, 2009Assignee: Bayer BioScience N.V.Inventors: Herman Van Mellaert, Johan Botterman, Jeroen Van Rie, Henk Joos
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Patent number: 7491536Abstract: Compositions and methods for protecting a plant from an insect pest are provided. In particular, novel polynucleotides and the pesticidal polypeptides they encode are provided. Methods of using the novel polynucleotides and pesticidal polypeptides of the invention to protect a plant from an insect pest are further provided. Particular embodiments of the invention provide pesticidal compositions and formulations, DNA constructs, and transformed plants, plant cells, and seeds.Type: GrantFiled: March 29, 2007Date of Patent: February 17, 2009Assignees: Pioneer Hi-Bred International, Inc., E.I. du Pont de Nemours and CompanyInventors: Andre R. Abad, Ronald D. Flannagan, Billy F. McCutchen, Cao Guo Yu
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Patent number: 7482432Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2 or 4, or the nucleotide sequence set forth in SEQ ID NO:1 or 3, as well as variants thereof.Type: GrantFiled: June 20, 2007Date of Patent: January 27, 2009Assignee: Athenix CorporationInventors: Nadine Carozzi, Tracy Hargiss, Michael G. Koziel, Nicholas B. Duck, Brian Carr
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Patent number: 7473821Abstract: Compositions and methods for protecting a plant from an insect pest are provided. Nucleic acid molecules encoding insect protoxins or insect toxins modified to comprise at least one proteolytic activation site that is sensitive to an insect gut protease are provided. Cleavage of a modified insect protoxin at the proteolytic activation site by an insect gut protease produces an active insect toxin in the gut of the insect pest. Cleavage of a modified insect toxin of the invention at a proteolytic activation site results in the production of an active insect toxin in the insect gut that displays improved pesticidal activity relative to the insect toxin that lacks the proteolytic activation site. Methods of using the modified insect protoxin and modified insect toxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided.Type: GrantFiled: June 7, 2006Date of Patent: January 6, 2009Assignees: Pioneer Hi-Bred International, Inc., E.I. du Pont de Nemours and CompanyInventors: Andre R. Abad, Gary C. Chun, Ronald D. Flannagan, Rafael Herrmann, Albert L. Lu, Janet A. Rice, Eric J. Schepers