Bacillus Thuringiensis Insect Toxin Patents (Class 536/23.71)
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Publication number: 20120272404Abstract: The present invention belongs to an insecticide compound derived from a strain of Bacillus thuringiensis and refers to the field of controlling plant pests, particularly to the control of the boll weevil—Anthonomus grandis. More specifically, the object of the invention refers to a new gene for the new delta-endotoxin designated Cry8Ha and to the cloning and expression of the gene that encodes for the protein Cry8Ha in Escherichia coli. There is provided the nucleotide sequence and encoding protein of the new delta-endotoxin, recombinant vectors and host cells. There is also provided processes and means for recombinant production and the use of the new delta-endotoxin for application in the control of the boll weevil. Additionally, the invention also provides an optimized synthetic gene for expression in cotton plants. Using the gene described herein, it is possible to transform plants based on techniques known by specialists in the art, for the expression of the endotoxin active against the boll weevil.Type: ApplicationFiled: July 26, 2010Publication date: October 25, 2012Applicant: EMBRAPA - Empresa Brasileira de Pesquisa AgropecuariaInventors: Maria Fatima Grossi De Sa, Gustavo Ramos De Oliveira, Maria Cristina Mattar Da Silva, Thales Lima Rocha, Mariana Torquato Quezado De Magalhaes
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Patent number: 8293998Abstract: The invention relates to the soybean variety designated A1024338. Provided by the invention are the seeds, plants and derivatives of the soybean variety A1024338. Also provided by the invention are tissue cultures of the soybean variety A1024338 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety A1024338 with itself or another soybean variety and plants produced by such methods.Type: GrantFiled: July 15, 2010Date of Patent: October 23, 2012Assignee: Monsanto Technology LLCInventor: Roger Lussenden
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Patent number: 8293997Abstract: The invention relates to the soybean variety designated A1024318. Provided by the invention are the seeds, plants and derivatives of the soybean variety A1024318. Also provided by the invention are tissue cultures of the soybean variety A1024318 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety A1024318 with itself or another soybean variety and plants produced by such methods.Type: GrantFiled: July 15, 2010Date of Patent: October 23, 2012Assignee: Monsanto Technology LLCInventors: Thomas Floyd, Barbara Schweigert
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Publication number: 20120266335Abstract: The invention provides synthetic nucleic acid sequences encoding proteins of interest that are particularly adapted to express well in plants. The claimed synthetic sequences utilize plant-optimized codons roughly in the same frequency at which they are utilized, on average, in genes naturally occurring in the plant species. The invention further includes synthetic DNA sequence for herbicide tolerance, water and/or heat stress tolerance, healthy oil modifications and for transformation marker genes and selectable marker genes are used. DNA construct and transgenic plants containing the synthetic sequences are taught as are methods and compositions for using the plants in agriculture.Type: ApplicationFiled: April 16, 2012Publication date: October 18, 2012Applicant: DOW AGROSCIENCES LLCInventors: IGNACIO M. LARRINUA, DONALD J. MERLO, AVUTU S. REDDY, ARVIND KUMAR THIRUMALAISWAMYSEKHAR, AARON T. WOOSLEY
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Publication number: 20120258910Abstract: The invention pertains to novel insecticidal compounds derived from Bacillus thuringiensis strains. New proteins designated Cry2Ae, Cry2Af, and Cry2Ag, and variants thereof are provided, as well as DNA sequences encoding these proteins or their variants. Further provided are recombinant hosts expressing such proteins, particularly plant cells and plants.Type: ApplicationFiled: May 7, 2012Publication date: October 11, 2012Applicant: Bayer CropScience N.V.Inventors: Greta Arnaut, Annemie Boets, Stijn Vanneste, Jeroen Van Rie, Sara Van Houdt
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Patent number: 8283524Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.Type: GrantFiled: May 6, 2009Date of Patent: October 9, 2012Assignee: Pioneer Hi-Bred International, IncInventors: Andre R. Abad, Hua Dong, Susan B. Lo, Mark Nelson, Xiaomei Shi, Clement W. K. Waine, Takashi Yamamoto
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Publication number: 20120255071Abstract: The invention provides nucleic acids obtained from strains of Bacillus thuringiensis encoding ?-endotoxins having pesticidal activity against insect pests including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acid molecules encoding mutant pesticidal polypeptides, pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid molecule of the invention. Such compositions find use in methods for controlling plant pests.Type: ApplicationFiled: March 30, 2012Publication date: October 4, 2012Applicant: Universidad Nacional Autonoma De MéxicoInventors: Mario Soberón-Chavez, Alejandra Bravo-De La Parra, Isabel Gómez-Gómez
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Publication number: 20120255065Abstract: Provided are pesticidal, particularly insecticidal, proteins which resemble S-layer proteins, as well as variants or mutants thereof, and DNAs encoding them. Further provided are methods and means for using said DNA or protein for controlling pests, particularly plant insect pests.Type: ApplicationFiled: April 17, 2012Publication date: October 4, 2012Applicant: Universidad Nacional Autonoma de MexicoInventors: Mario SOBERON-CHAVEZ, Alejandra Bravo-De-La-Parra
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Patent number: 8269069Abstract: The present invention includes domain I modifications that improve various attributes of various Coleopteran-active Cry proteins. These attributes can include improved target pest spectrum, potency, and insect resistance management. The subject modifications can affect protoxin activation and the efficiency of pore formation, which can lead to enhanced insect intoxication.Type: GrantFiled: July 30, 2009Date of Patent: September 18, 2012Assignee: Dow AgroSciences, LLCInventors: Kenneth Narva, Ignacio M. Larrinua, Timothy D. Hey
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Publication number: 20120233726Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.Type: ApplicationFiled: March 7, 2012Publication date: September 13, 2012Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ANDRE R. ABAD, HUA DONG, SUE B. LO, XIAOMEI SHI, THOMAS C. WOLFE
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Patent number: 8252972Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.Type: GrantFiled: January 6, 2010Date of Patent: August 28, 2012Assignee: Pioneer Hi Bred International IncInventors: Andre R. Abad, Hua Dong, Deirdre M. Kapka-Kitzman, Sue B. Lo, Xiaomei Shi
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Publication number: 20120210464Abstract: Improved compositions and methods for controlling pests are disclosed. In particular, novel engineered Cry1Ba (eCry1Ba) proteins having improved toxicity to lepidopteran insect pests are provided. By substituting at least one amino acid in domain I of a Cry1Ba protein an engineered Cry1Ba protein having substantially altered insecticidal properties is designed. Further, a method of making the engineered Cry1Ba proteins and methods of using the ecry1Ba nucleic acid sequences, for example in transgenic plants to express eCry1B proteins to confer protection from insect damage are disclosed.Type: ApplicationFiled: September 27, 2010Publication date: August 16, 2012Applicant: SYNGENTA PARTICIPATIONS AGInventors: Yan Gao, Jared Conville, Jeng Shong Chen
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Publication number: 20120210462Abstract: Traditionally, the primary method for impacting insect pest populations is the application of broad-spectrum chemical insecticides. However, there is increasing concern about the environmental hazards associated with the production and use of synthetic chemical pesticides. Thus, there is substantial interest in developing alternative pesticides, including biological control of insect pests of agricultural significance using a microbial agent or another species of insect. The present invention provides compositions and methods for such biological control. Modified Cry3 pesticidal polypeptides, polynucleotides encoding such polypeptides, and methods of use are disclosed. The modified polynucleotides provided herein can be used to transform organisms and cells of hosts comprising plant, insects, and microorganisms. The expression of modified polypeptides can provide the host with improved insecticidal activity against one or more insect pathogens.Type: ApplicationFiled: February 10, 2012Publication date: August 16, 2012Applicant: Pioneer Hi-Bred International, Inc.Inventors: Ericka Bermudez, Ruth Cong, Jingtong Hou, Takashi Yamamoto
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Patent number: 8237021Abstract: The present invention relates to the field of plant pest control, particularly insect control. Provided are nucleotide sequences from Bacillus thuringiensis encoding insecticidal proteins. Further provided are methods and means for using said nucleotide sequence for controlling plant insect pests.Type: GrantFiled: June 25, 2010Date of Patent: August 7, 2012Assignee: Bayer CropScience N.V.Inventors: Greta Arnaut, Annemie Boets, Karel De Rudder, Stijn Vanneste, Jeroen Van Rie
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Publication number: 20120192310Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.Type: ApplicationFiled: January 23, 2012Publication date: July 26, 2012Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Andre R. Abad, Hua Dong, Sue B. Lo, Xiaomei Shi, Thomas C. Wolfe
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Patent number: 8222488Abstract: A method of enhancing expression of at least one desired gene product in a plant, comprises transgenically introducing a Tombusvirus p19/R43W mutant gene (SEQ. ID NO: 3) into the plant, expressing at least one desired gene product in the plant, wherein expression of said product is susceptible to RNA silencing; and co-expressing the transgenically introduced p19/R43W, to produce an amount of P19/R43W mutant protein (SEQ. ID NO: 4) sufficient to suppress silencing of expression of said desired gene product.Type: GrantFiled: February 5, 2007Date of Patent: July 17, 2012Assignee: Texas A&M University SystemInventor: Herman B. Scholthof
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Publication number: 20120156167Abstract: The isolation, cloning of an enhancin gene bel1 from Bacillus thuringiensis, and the use of an enhancin Bel1 in biological insecticides are disclosed in the present invention. A new enhancin gene bel1 is isolated from Bacillus thuringiensis subsp. kurstaki YBT-1520, and its encoded product is a new enhancin Bel1. The enhancin has very strong effect-enhancing activity on the insecticidal action of insecticidal crystal protein Cry1Ac in killing Lepidoptera insects. The insecticidal activity of genetically engineered Bacillus thuringiensis BMB0187 developed on the basis of this enhancin is 5.7 times as high as that of the initial strain.Type: ApplicationFiled: November 23, 2009Publication date: June 21, 2012Applicant: HUAZHONG AGRICULTURAL UNIVERSITYInventors: Ming Sun, Ziniu Yu, Shangling Fang, Donghai Peng, Lifang Ruan, Shouwen Chen, Chunping Luo
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Patent number: 8198513Abstract: The invention pertains to novel insecticidal compounds derived from Bacillus thuringiensis strains. New proteins designated Cry1Jd, Cry9Fa, and Cry1Bf, and insecticidal fragments thereof are provided, as well as DNA sequences encoding these proteins or their insecticidal fragments. Further provided are recombinant hosts expressing such proteins, particularly plant cells and plants.Type: GrantFiled: June 1, 2011Date of Patent: June 12, 2012Assignee: Bayer CropScience N.V.Inventors: Annemie Boets, Nicole Damme, Eva Mathieu, Stijn Vanneste, Jeroen Van Rie
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Publication number: 20120117690Abstract: The present invention provides insecticidal polypeptides related to shuffled Bacillus thuringiensis Cry1 polypeptides. Nucleic acids encoding the polypeptides of the invention are also provided. Methods for using the polypeptides and nucleic acids of the invention to enhance resistance of plants to insect predation are encompassed.Type: ApplicationFiled: January 24, 2012Publication date: May 10, 2012Applicant: Pioneer Hi-Bred International, Inc.Inventors: David Cerf, Ruth Cong, Michael Freeman, Kevin McBride, Takashi Yamamoto
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Patent number: 8173872Abstract: The invention pertains to novel insecticidal compounds derived from Bacillus thuringiensis strains. New proteins designated. Cry2Ae, Cry2Af, and Cry2Ag, and variants thereof are provided, as well as DNA sequences encoding these proteins or their variants. Further provided are recombinant hosts expressing such proteins, particularly plant cells and plants.Type: GrantFiled: September 23, 2010Date of Patent: May 8, 2012Assignee: Bayer CropScience NVInventors: Greta Arnaut, Annemie {acute over (B)}oets, Stijn Vanneste, Jeroen Van Rie, Sara Van Houdt
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Patent number: 8173871Abstract: Provided are pesticidal, particularly insecticidal, proteins which resemble S- layer proteins, as well as variants or mutants thereof, and DNAs encoding them. Further provided are methods and means for using said DNA or protein for controlling pests, particularly plant insect pests.Type: GrantFiled: July 4, 2006Date of Patent: May 8, 2012Assignee: Universidad Nacional Autonoma de MexicoInventors: Mario Soberon-Chavez, Alejandra Bravo-De-La-Parra
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Publication number: 20120110706Abstract: The subject invention concerns plants protected from nematode feeding damage and improved versions of Cry proteins. The subject invention also concerns improved versions of Cry21A proteins. Synthetic genes encoding these modified proteins are also part of the subject invention. Another embodiment of the subject invention includes plants transformed with the genes of the subject invention. In yet another embodiment the subject invention concerns Bt proteins for in-plant protection against crop damage by root knot nematode (RKN; Meloidogyne incognita) and soybean cyst nematode (SCN; Heterodera glycines).Type: ApplicationFiled: August 25, 2009Publication date: May 3, 2012Applicant: Dow AgroSciences LLCInventors: Timothy D. Hey, Kenneth Narva, Aaron T. Woosley
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Publication number: 20120073016Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions including a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules having nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, the nucleotide sequence set forth in SEQ ID NO:1, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12, as well as variants and fragments thereof.Type: ApplicationFiled: November 22, 2011Publication date: March 22, 2012Inventors: Kimberly S. Sampson, Daniel J. Tomso, Volker Heinrichs
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Publication number: 20120066793Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:13-24 or the nucleotide sequence set forth in SEQ ID NO:1-12 and 25-44, as well as variants and fragments thereof.Type: ApplicationFiled: November 21, 2011Publication date: March 15, 2012Applicant: Athenix CorpInventors: Kimberly S. Sampson, Daniel John Tomso
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Patent number: 8129593Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.Type: GrantFiled: April 3, 2009Date of Patent: March 6, 2012Assignee: Pioneer Hi-Bred International, Inc.Inventors: Andre R. Abad, Hua Dong, Sue B. Lo, Xiaomei Shi
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Publication number: 20120047605Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.Type: ApplicationFiled: August 12, 2011Publication date: February 23, 2012Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Andre R. Abad, Hua Dong, Sue B. Lo, Xiaomei Shi, Thomas C. Wolfe
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Publication number: 20120047607Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Coleoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.Type: ApplicationFiled: August 17, 2011Publication date: February 23, 2012Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: André R. Abad, Hua Dong, Sue B. Lo, Xiaomei Shi
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Publication number: 20120047606Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.Type: ApplicationFiled: August 15, 2011Publication date: February 23, 2012Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Andre R. Abad, Hua Dong, Sue B. Lo, Xiaomei Shi, Thomas C. Wolfe
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Patent number: 8101826Abstract: The present invention discloses methods and compositions comprising a group of novel expression cassettes which provide significantly improved levels of accumulation of Coleopteran inhibitory Cry3B and Cry3B variant amino acid sequences when these are expressed in plants. The preferred embodiments of the invention provide at least up to ten fold higher levels of insect controlling protein relative to the highest levels obtained using prior compositions. In particular, transgenic maize expressing higher levels of a protein designed to exhibit increased toxicity toward Coleopteran pests deliver superior levels of insect protection and are less likely to sponsor development of populations of target insects that are resistant to the insecticidally active protein.Type: GrantFiled: March 30, 2010Date of Patent: January 24, 2012Assignee: Monsanto Technology LLCInventor: Charles P. Romano
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Patent number: 8101825Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequences shown in SEQ ID NOS:3 and 5, and the nucleotide sequences set forth in SEQ ID NOS:1, 2, and 4, as well as variants and fragments thereof.Type: GrantFiled: February 15, 2008Date of Patent: January 24, 2012Assignee: Athenix Corp.Inventors: Nadine Carozzi, Tracy Hargiss, Michael G. Koziel, Nicholas B. Duck, Brian Carr
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Patent number: 8076533Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:13-24 or the nucleotide sequence set forth in SEQ ID NO:1-12 and 25-44, as well as variants and fragments thereof.Type: GrantFiled: February 15, 2011Date of Patent: December 13, 2011Assignee: Athenix Corp.Inventors: Kimberly S. Sampson, Daniel John Tomso
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Publication number: 20110288272Abstract: Methods for making a modified Cry3A toxin are disclosed. Such methods include the insertion of a protease recognition site that is recognized by a gut protease of a target insect, such as corn rootworm, into at least one position of a Cry3A toxin so that a modified Cry3A toxin is thus designed. The coding sequence of the modified Cry3A toxin may be transformed into a host cell and the host cell grown under conditions that allow the host cell to produce the modified Cry3A toxin. The host cell may be a plant cell and the plant may be comprised in a transgenic plant. Thus, the transgenic plant may be used to produce the modified Cry3A toxin.Type: ApplicationFiled: July 25, 2011Publication date: November 24, 2011Inventors: Eric Chen, Cheryl Stacy
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Publication number: 20110289627Abstract: Methods for making a modified Cry3A toxin are disclosed. Such methods include the insertion of a protease recognition site that is recognized by a gut protease of a target insect, such as corn rootworm, into at least one position of a Cry3A toxin so that a modified Cry3A toxin is thus designed. The coding sequence of the modified Cry3A toxin may be transformed into a host cell and the host cell grown under conditions that allow the host cell to produce the modified Cry3A toxin. The host cell may be a plant cell and the plant may be comprised in a transgenic plant. Thus, the transgenic plant may be used to produce the modified Cry3A toxin.Type: ApplicationFiled: July 25, 2011Publication date: November 24, 2011Inventors: Eric Chen, Cheryl Stacy
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Publication number: 20110283417Abstract: The present invention relates to the isolation and characterization of nucleotide sequences encoding novel insecticidal proteins secreted into the extracellular space from Bacillus thuringiensis and related strains. The proteins are isolated from culture supernatants of Bacillus thuringiensis and related strains and display insecticidal activity against lepidopteran insects including European corn borer (ECB), tobacco budworm (TBW) and diamondback moth (DBM). Insecticidal proteins encoded by nucleotide sequences that hybridize under stringent conditions to the isolated and characterized nucleotide sequences are disclosed. Methods are disclosed for making and using transgenic cells and plants comprising the novel nucleotide sequence of the invention.Type: ApplicationFiled: June 29, 2011Publication date: November 17, 2011Inventors: JUDITH DONOVAN, WILLIAM DONOVAN, JAMES ENGLEMAN, THOMAS MALVAR, JOHN PITKIN
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Patent number: 8049071Abstract: The present invention provides a transgenic soybean event MON87701, and cells, seeds, and plants comprising DNA diagnostic for the soybean event. The invention also provides compositions comprising nucleotide sequences that are diagnostic for said soybean event in a biological sample, probes and primers for use in detecting nucleotide sequences that are diagnostic for the presence of said soybean event in a biological sample, and methods for detecting the presence of said soybean event nucleotide sequences in a biological sample. The invention further provides methods of growing the seeds of such soybean event into soybean plants, and methods of breeding to produce soybean plants comprising DNA diagnostic for the soybean event.Type: GrantFiled: November 6, 2008Date of Patent: November 1, 2011Assignee: Monsanto Technology LLCInventors: Ai-Guo Gao, Kathryn H. Kolacz, Ted C. MacRae, John A. Miklos, Mark S. Paradise, Frederick J. Perlak, Andrea S. Toedebusch, Leslie A. Harrison
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Publication number: 20110263488Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed.Type: ApplicationFiled: June 24, 2011Publication date: October 27, 2011Applicant: ATHENIX CORP.Inventors: Nadine Carozzi, Michael G. Koziel, Nicholas B. Duck, Nalini M. Desai, Rong Guo, Daniel John Tomso, Rebekah Deter, Tracy Hargiss
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Patent number: 8044266Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin and delta-endotoxin-associated polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin and delta-endotoxin-associated nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequences shown in SEQ ID NOS:3, 5, and 7, and the nucleotide sequences set forth in SEQ ID NO:1, 2, 4, and 6, as well as variants and fragments thereof.Type: GrantFiled: February 5, 2008Date of Patent: October 25, 2011Assignee: Athenix Corp.Inventors: Nadine Carozzi, Tracy Hargiss, Michael G. Koziel, Nicholas B. Duck, Brian Carr
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Patent number: 8034997Abstract: The present invention provides nucleotide sequences encoding an insecticidal protein exhibiting lepidopteran inhibitory activity, as well as a novel insecticidal protein referred to herein as a Cry1A.105 insecticide, transgenic plants expressing the insecticide, and methods for detecting the presence of the nucleotide sequences or the insecticide in a biological sample.Type: GrantFiled: August 30, 2006Date of Patent: October 11, 2011Assignee: Monsanto Technology LLCInventors: Natalia N. Bogdanova, David R. Corbin, Thomas M. Malvar, Frederick J. Perlak, James K. Roberts, Charles P. Romano
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Publication number: 20110239334Abstract: The subject invention concerns plants protected from nematode feeding damage and improved versions of Cry proteins. Synthetic genes encoding Cry proteins are also part of the subject invention. Another embodiment of the subject invention includes plants transformed with the genes of the subject invention. In yet another embodiment the subject invention concerns Bt proteins for in-plant protection against crop damage by root knot nematode (RKN; Meloidogyne incognita) and soybean cyst nematode (SCN; Heterodera glycines).Type: ApplicationFiled: August 25, 2009Publication date: September 29, 2011Applicant: Dow AgroSciences LLCInventors: Timothy D. Hey, Aaron T. Woosley, Kenneth E. Narva
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Publication number: 20110231963Abstract: The subject invention concerns plants protected from nematode feeding damage and improved versions of Cry proteins. The subject invention also concerns improved versions of Cry14A proteins. Synthetic genes encoding these modified proteins are also part of the subject invention. Another embodiment of the subject invention includes plants transformed with the genes of the subject invention. In yet another embodiment the subject invention concerns Bt proteins for in-plant protection against crop damage by root knot nematode (RKN; Meloidogyne incognita) and soybean cyst nematode (SCN; Heterodera glycines).Type: ApplicationFiled: August 25, 2009Publication date: September 22, 2011Applicant: Dow AgroSciences LLCInventors: Timothy D. Hey, Knneth Narva, Aaron T. Woosley
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Patent number: 8017387Abstract: Polynucleotides encoding telomerase reverse transcriptase (TERT) fusion proteins are provided, the TERT fusion proteins comprising a TERT protein, or functional variant thereof, fused to a substantial portion of the B subunit of heat labile enterotoxin (LTB). TERT variants useful in TERT-LTB fusion proteins of the invention comprise mutations that function to eliminate telomerase catalytic activity. The polynucleotides of the present invention can elicit an immune response in a mammal, which, in preferred embodiments, is stronger than the immune response elicited by a wild-type TERT. TERT expression is commonly associated with the development of human carcinomas. The present invention provides compositions and methods to elicit or enhance immunity to the protein product expressed by the TERT tumor-associated antigen, wherein aberrant TERT expression is associated with a carcinoma or its development.Type: GrantFiled: October 11, 2007Date of Patent: September 13, 2011Assignee: Istituto di Ricerche di Biologia Molecolare P. Angeletti SpAInventors: Elisa Scarselli, Carmela Mennuni, Nicola La Monica, Gennaro Ciliberto
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Publication number: 20110197314Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:13-24 or the nucleotide sequence set forth in SEQ ID NO:1-12 and 25-44, as well as variants and fragments thereof.Type: ApplicationFiled: February 15, 2011Publication date: August 11, 2011Applicant: Athenix Corp.Inventors: Kimberly S. Sampson, Daniel John Tomso
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Patent number: 7985892Abstract: This invention provides truncated Cry35 proteins that surprisingly and unexpectedly have increased pesticidal activity as compared to full-length Cry35 proteins. The subject invention also includes polynucleotides that encode these truncated proteins, transgenic plants comprising a truncated gene of the subject invention, and transgenic plants that produce these truncated proteins. This invention further provides methods of controlling plant pests, including rootworms, with these truncated proteins. The truncated Cry35 proteins of the subject invention are preferably used in combination with Cry34 proteins, which are known in the art. Various surprising advantages of the subject invention will be apparent in light of this disclosure.Type: GrantFiled: June 28, 2005Date of Patent: July 26, 2011Assignee: Dow AgroSciences LLCInventors: Yong Gao, Rod Aaron Herman, Andrew William Carr, Deborah Ann Schwedler, Xioaping Xu, George Erwin Schwab
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Publication number: 20110154524Abstract: The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-032316-8 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.Type: ApplicationFiled: December 16, 2010Publication date: June 23, 2011Applicants: PIONEER HI-BRED INTERNATIONAL, INC., E.I. DU PONT DE NEMOURS AND COMPANYInventors: Scott Diehn, Albert L. Lu, Timothy M. Nowatzki, Douglas S. Nubel, M. Alejandra Pascual, James C. Register, III, Christopher J. Scelonge, Gregory J. Young, Joshua K. Young, Cathy Xiaoyan Zhong
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Publication number: 20110154525Abstract: The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-040416-8 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.Type: ApplicationFiled: December 16, 2010Publication date: June 23, 2011Applicants: PIONEER HI-BRED INTERNATIONAL, INC., E.I. DU PONT DE NEMOURS AND COMPANYInventors: Scott Diehn, Albert L. Lu, Timothy M. Nowatzki, Douglas S. Nubel, M. Alejandra Pascual, James C. Register, III, Christopher J. Scelonge, Gregory J. Young, Joshua K. Young, Cathy Xiaoyan Zhong
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Publication number: 20110154535Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Coleoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.Type: ApplicationFiled: December 8, 2010Publication date: June 23, 2011Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Andre Roger Abad, Hua Dong, Sue B. Lo, Xiaomei Shi, Thomas C. Wolfe
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Patent number: 7964773Abstract: The present invention relates to new DNA sequences encoding an insecticidal Cry9Fa protein and insecticidal parts thereof, which are useful to protect plants from insect damage. Also included herein are micro-organisms and plants transformed with a DNA sequence encoding an insecticidal Cry9Fa protein and processes for controlling insects and to obtain a plant resistant to insects.Type: GrantFiled: April 11, 2008Date of Patent: June 21, 2011Assignee: Bayer Bioscience N.V.Inventors: Annemie Boets, Nicole Damme, Eva Mathieu, Stijn Vanneste, Jeroen Van Rie
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Patent number: 7939651Abstract: This invention provides modified, insecticidal Cry35 proteins with enhanced properties as compared to wild-type Cry35 proteins. The modifications to these proteins were based in part on analysis of the atomic coordinates and three-dimensional (3D) structure of the ˜45 kDa 149B1 protein and other proteins in the Cry35 class. The subject invention also includes polynucleotides that encode these modified proteins, and transgenic plants that produce these modified proteins. This invention further provides methods of controlling plant pests, including rootworms, with these modified proteins. The modified proteins of the subject invention include chimeric toxins involving exchanged segments, domains, and motifs as discussed herein. The subject invention also provides methods of modifying Cry35 proteins.Type: GrantFiled: March 2, 2010Date of Patent: May 10, 2011Assignee: Dow AgroSciences LLCInventors: H. Ernest Schnepf, Kenneth E. Narva, Steven L. Evans
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Patent number: 7923602Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences.Type: GrantFiled: June 14, 2007Date of Patent: April 12, 2011Assignee: Athenix Corp.Inventors: Nadine Carozzi, Nicholas Duck, Theodore Kahn, Nalini Desai
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Patent number: 7919322Abstract: The present invention provides methods, nucleic acid constructs, and kits for selectively deleting a region of a nucleic acid sequence. Specifically, it utilizes retargeting retroelements to place site-specific recombination sites at targeted locations in the nucleic acid sequence. The region between the recombination sites is then deleted using a site-specific recombination system.Type: GrantFiled: March 29, 2006Date of Patent: April 5, 2011Assignee: Sigma-Aldrich Co.Inventors: Melissa Spears, Greg Davis, Kevin Kayser