Primers Patents (Class 536/24.33)
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Patent number: 8440396Abstract: This invention relates to the use of tumor-derived or associated extracellular ribonucleic acid (RNA) found circulating in the plasma or serum fraction of blood for the detection, monitoring, or evaluation of cancer or premalignant conditions. Specifically, this invention enables the extraction of circulating RNA from plasma or serum and utilizes nucleic acid amplification assays for the identification, detection, inference, monitoring, or evaluation of any neoplasm, benign, premalignant, or malignant, in humans or other animals, which might be associated with that RNA. Further, this invention allows the qualitative or quantitative detection of tumor-derived or associated extracellular RNA circulating in the plasma or serum of humans or animals with or without any prior knowledge of the presence of cancer or premalignant tissue.Type: GrantFiled: November 2, 2006Date of Patent: May 14, 2013Assignee: OncoMedx, Inc.Inventor: Michael S. Kopreski
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Patent number: 8440802Abstract: The present invention provides for selection of bovine animals that will display phenotypes associated with increased rates of growth. These phenotypes include hot carcass weight, average daily gain, shipping weight, end of test rib eye area, and adjusted weaning weight which is a measure of post-natal growth, based on the knowledge of their CRH, POMC and MC4R genotypes. The predictive value comes from the discovery that certain single nucleotide polymorphisms (SNPs) in these genes are linked to higher growth rate phenotypes. Specifically, the phenotypes that correlated with specific SNP's are end-of-test rib-eye area, adjusted weaning weight, average daily gain, shipping weight and hot carcass weight. The invention also provides novel kits that can be used in making the determination of these genotypes.Type: GrantFiled: July 12, 2011Date of Patent: May 14, 2013Assignee: University of SaskatchewanInventors: Fiona Buchanan, Tracy D. Thue, Dianne Winkelman-Sim
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Patent number: 8440429Abstract: An automated method for preparing and amplifying a sequence contained in a nucleic acid present in a sample, the nucleic acid being prepared in a receptacle that is part of a unit that includes a plurality of receptacles and holds a removable contact-limiting element for aspirating a fluid component of the sample from the receptacle.Type: GrantFiled: September 30, 2008Date of Patent: May 14, 2013Assignee: Gen-Probe IncorporatedInventors: Kelly G. Ammann, Ralph E. Burns, Ernest V. Hansberry, Glenn A. Horner, Cheryl A. Jakub, John E. Kling, Donald J. Nieglos, Robert E. Schneider, Robert J. Smith
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Publication number: 20130115614Abstract: A primer pair is provided capable of amplifying partial sequences of endogenous wheat DNA which are single copies and which allow wheat to be specifically detected without cross-reacting with other plants in a polymerase chain reaction. Also provided is a kit for detecting or assaying an endogenous wheat DNA sequence in a test sample by a polymerase chain reaction.Type: ApplicationFiled: December 11, 2012Publication date: May 9, 2013Inventors: Akihiro Hino, Takashi Kodama, Maya Lida, Hirohito Yamakawa, Satomi Nozaki, Katsuyuki Hayakawa
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Publication number: 20130115693Abstract: The present invention refers to a device, comprising a hollow body having at least one open end comprising at least one solid matrix binding, adsorbing, absorbing, chelating or retaining compounds which are not desired in a sample and preferably at least one barrier which is non-permeable for liquids and solids under ambience conditions, however, becomes liquid-permeable by applying an external force to said barrier, the use of such a device for isolating or purifying a biomolecule from a sample, a method for preparation of said device and a method for isolation or purification of any biomolecule using said device.Type: ApplicationFiled: July 13, 2011Publication date: May 9, 2013Inventors: Vera Holländer, Markus Müller, Karin Schulte
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Publication number: 20130112152Abstract: The present disclosure relates to single nucleotide polymorphisms and primers and probes useful for screening for, diagnosing or detecting congenital stationary night blindness or for selecting or detecting horse coat patterns.Type: ApplicationFiled: November 9, 2011Publication date: May 9, 2013Inventors: Rebecca Bellone, Sheila Archer, Claire Wade, Bruce Grahn, Lynne Sandmeyer, George Forsyth
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Publication number: 20130116149Abstract: Genetic markers associated with intellectual disability as well as compositions, methods and kits for screening for genetic markers intellectual disability, diagnosing intellectual disability and identifying individuals with a predisposition for offspring suffering from intellectual disability are provided.Type: ApplicationFiled: June 23, 2011Publication date: May 9, 2013Applicant: CENTRE FOR ADDICTION AND MENTAL HEALTHInventor: John B. Vincent
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Patent number: 8435960Abstract: The present invention provides devices and compositions for the management of infection of topical lesions, each of the devices and compositions containing protonated/acidified nucleic acids either on its surface, or integrated into the device. These modified nucleic acids are effective as bactericidal and/or bacteriostatic agents without regard to the class of bacteria, so are especially useful when diagnosis is difficult or when multiple infectious organisms are present. The antibiotic activity of nucleic acids of the invention is not dependent on either the specific sequence of the nucleic acid or the length of the nucleic acid molecule. The nucleic acids used in the invention are protonated/acidified to give a pH when dissolved in water of less than pH 7 to about 1, more preferably less than pH 4.5 to about 1, and even more preferably less than pH 2 to about 1.Type: GrantFiled: May 30, 2008Date of Patent: May 7, 2013Assignee: Lakewood-Amedex, Inc.Inventors: Roderic M. K. Dale, Steven L. Gatton, Amy Arrow
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Patent number: 8435737Abstract: Nucleic acid sequences are provided which in an embodiment provide a primer pair. The primers are capable of amplifying a nucleic acid molecule that indicates the presence of a propane-oxidizing and/or butane-oxidizing microorganism. A method is provided which employs such primers in a process that indicates the presence of such organisms. The method is useful in detecting the presence of petroleum-like products.Type: GrantFiled: June 1, 2011Date of Patent: May 7, 2013Assignee: Cal Poly CorporationInventors: Raul Cano, Christopher Kitts, Brian Jeremy Chan
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Patent number: 8436163Abstract: Antisense compositions targeted against an mRNA sequence coding for a selected protein, at a region having its 5? end from 1 to about 25 base pairs downstream of a normal splice acceptor junction in the preprocessed mRNA, are disclosed. The antisense compound is RNase-inactive, and is preferably a phosphorodiamidate-linked morpholino oligonucleotide. Such targeting is effective to inhibit natural mRNA splice processing, produce splice variant mRNAs, and inhibit normal expression of the protein.Type: GrantFiled: August 26, 2011Date of Patent: May 7, 2013Assignee: AVI BioPharma, Inc.Inventors: Patrick L. Iversen, Robert Hudziak
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Patent number: 8435732Abstract: A recombinant DNA construct, recombinant vectors and host cells comprising the dimers of DNA A and DNA B of Mungbean Yellow Mosaic India Virus (MYMIV) in a single Ti plasmid are provided herein.Type: GrantFiled: December 8, 2008Date of Patent: May 7, 2013Assignee: National Institute for Plant Genome ResearchInventors: Debasis Chattopadhyay, Rajiv Kumar Yadav, Rakesh Kumar Shukla
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Patent number: 8435740Abstract: A method for the identification of DNA sequence elements in complex and highly variable sequences is described. The method consists of identifying a short sequence element of several DNA bases (2-6 bases) at a given position in the genome simultaneously on all parental alleles. The method allows differentiating mini-haplotypes on different alleles in one analysis. The method consists of carrying out an enzymatic primer extension reaction with a combination of extension primers (pool of primers) and analyzing the products by mass spectrometry. The pool of primers is assembled in such a way that the primer extension product allows unambiguous identification of both the primer of the pool that was extended and the base that was added. The method of great utility for DNA sequences harboring many SNP's close to each other with many possible haplotypes. Such sequences are known in the Major Histocompatibility Complex (MHC).Type: GrantFiled: September 14, 2010Date of Patent: May 7, 2013Assignee: Commissariat a l'Energie Atomique et Aux Energies AlternativesInventors: Ivo Glynne Gut, Ramon Kucharzak
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Patent number: 8435743Abstract: The present invention provides a cotton plant event MON 88913 compositions and seed. Also provided are assays for detecting the presence of the cotton plant event MON 88913 based on a DNA sequence and the use of this DNA sequence as a molecular marker in a DNA detection method.Type: GrantFiled: November 4, 2011Date of Patent: May 7, 2013Assignee: Monsanto Technology LLCInventors: R. Eric Cerny, Can Duong, Jesse L. Hart, Scott A. Huber, Rachel L. Krieb, Jennifer J. Listello, Amy B. Martens, Bernard Sammons
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Publication number: 20130108642Abstract: The invention provided Mucorales CotH polypeptides and encoding nucleic acid molecules. The Mucorales CotH polypeptides and encoding nucleic acids can be advantageously used to diagnose, treat or prevent fungal conditions, in particular mucormycosis.Type: ApplicationFiled: September 14, 2012Publication date: May 2, 2013Inventors: Ashraf S. Ibrahim, Mingfu Liu, Teklegiorgis Ghebremariam, Yue Fu, John E. Edwards, Scott Filler
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Publication number: 20130109579Abstract: The methods and compositions provided herein relate to the discovery of 13 STR markers, found on the human Y chromosome, having surprisingly high mutation rates when compared with 173 other Y-STR markers known today. The set of RM-Y-STRs may overcome the current dilemma of Y-chromosome analysis in forensic applications due to their extraordinary mutation properties. Embodiments of the invention include methods for allelic determination of rapidly-mutating Y-STR markers, amplification primers for the analysis of rapidly-mutating Y-STR markers, allelic ladders for analysis of rapidly-mutating Y-STR markers, and kits for the analysis of rapidly-mutating Y-STR markers.Type: ApplicationFiled: September 13, 2012Publication date: May 2, 2013Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Rixun FANG, Manohar Furtado, Manfred Kayser, Kaye Ballantyne
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Publication number: 20130109023Abstract: The present teachings provide methods, compositions, and kits for nucleic acid amplification. In some embodiments of the present teachings, amplification reactions are performed with at least one high stability primer. In some embodiments, the present teachings provide a method comprising a high stability primer for amplification of a nucleic acid sequence in a sample comprising a target nucleic acid sequence and a PCR inhibitor.Type: ApplicationFiled: August 24, 2012Publication date: May 2, 2013Applicant: APPLIED BIOSYSTEMS LLCInventors: Julio J. Mulero, Lori K. Hennessy
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Patent number: 8431340Abstract: Methods and devices for the interfacing of microchips to various types of modules are disclosed. The technology disclosed can be used as sample preparation and analysis systems for various applications, such as DNA sequencing and genotyping, proteomics, pathogen detection, diagnostics and biodefense.Type: GrantFiled: October 23, 2009Date of Patent: April 30, 2013Assignee: IntegenX Inc.Inventors: Stevan Bogdan Jovanovich, Iuliu Ioan Blaga
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Publication number: 20130102490Abstract: The present invention provides a method for counting the absolute copy number of a nucleic acid sequence in a cell, which comprises the following steps: (i) dividing a lysate of the cell or a lysate of a sample of the cell into a plurality aliquots: (ii) providing conditions suitable for the amplification of the nucleic acid sequence in each aliquot: (iii) counting the number of aliquots in which the nucleic acid was amplified in step (ii) and directly deducing the copy number of the nucleic acid sequence in a cell. The method may be used to count chromatid copy number, for example to investigate the ploidy of a cell such as an oocyte or an embryo-derived cell.Type: ApplicationFiled: May 4, 2011Publication date: April 25, 2013Applicant: MEDICAL RESEARCH COUNCILInventors: Angelika H. Daser, Paul H. Dear
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Publication number: 20130101989Abstract: Described is a diagnostic method for predicting the response of a patient to chemovirotherapy or radiovirotherapy, comprising exposing primary tumor cells from a patient, e.g., tumor cells obtained from a brain tumor or pancreatic cancer, to (i) a parvovirus and/or (ii) a chemotherapeutic agent or radiotherapy, and determining the reduction of the expression or concentration of ISG15.Type: ApplicationFiled: April 29, 2011Publication date: April 25, 2013Applicants: DEUTSCHES KREBSFORSCHUNGSZENTRUM STIFUNG DES OEFFE, UNIVERSITAETSKLINIKUM HEIDELBERG, RUPRECHT-KARIS-UNIVERSITAET HEIDELBERGInventors: Nathalia Giese, Jens Werner, Markus Buechler, Thomas Giese, Laurent Daeffler, Celina Cziepluch, Jean Rommelaere, Zahari Raykov
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Patent number: 8426137Abstract: Compositions and methods for the detection of vancomycin-resistant pathogens using primers and/or probes to the vanA and vanB genes.Type: GrantFiled: July 5, 2011Date of Patent: April 23, 2013Assignee: Genohm Sciences Canada, Inc.Inventors: Michel G. Bergeron, Maurice Boissinot, Ann Huletsky, Christian Menard, Marc Ouellette, Francois J. Picard, Paul H. Roy
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Patent number: 8426129Abstract: The present invention relates to the typing of HLA alleles. The sequence of exon 2 and exon 3 of the alleles HLA-B*3913, HLA-B*1406 and HLA-B*51new and of exon 2 of the alleles HLA-DRB1*0820, HLA-DRB1*04new and HLA-DRB4*01new are disclosed. The present invention relates to methods for typing of the alleles HLA-B*3913, HLA-B*1406 and HLA-B*51new. The present invention further provides primers and probes to be used in the methods for typing. A diagnostic kit comprising these primers and probes is also part of the present invention.Type: GrantFiled: April 14, 2008Date of Patent: April 23, 2013Assignee: Innogenetics N.V.Inventors: Ilse De Canck, Guy Mersch, Rudi Rossau
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Publication number: 20130095485Abstract: This application provides materials and methods for the detection of the aad-12 gene and event pDAB4472-1606 in biological samples derived from plants.Type: ApplicationFiled: October 18, 2012Publication date: April 18, 2013Applicant: DOW AGROSCIENCES LLCInventor: Dow Agrosciences LLC
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Publication number: 20130095494Abstract: This invention features systems and methods for the detection of analytes, and their use in the treatment and diagnosis of disease.Type: ApplicationFiled: October 11, 2012Publication date: April 18, 2013Applicant: T2 Biosystems, Inc.Inventor: T2 Biosystems, Inc.
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Publication number: 20130095486Abstract: This application provides materials and methods for the detection of aad-12 gene events in biological samples derived from recombinant plants and a materials and methods for the detection of contaminating events in samples derived from recombinant plants.Type: ApplicationFiled: October 18, 2012Publication date: April 18, 2013Applicant: DOW AGROSCIENCES LLCInventor: Dow Agrosciences LLC
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Publication number: 20130096018Abstract: The present invention provides a method for predicting the survival rate and prognosis of esophageal carcinoma patients, which is characterized in examining the expression level of a specific gene, peptidase inhibitor 3 (PI3) or CD14 antigen (CD14) in a sample, and comparing to the average expression level of said specific gene from patients to determine the survival and prognosis status for esophageal cancer. The present invention further provides a kit for predicting the survival rate and prognosis of esophageal carcinoma patients.Type: ApplicationFiled: March 21, 2012Publication date: April 18, 2013Applicant: KAOHSIUNG MEDICAL UNIVERSITYInventors: Ming-Tsang Wu, Deng-Chyang Wu, Hung-Ju Su, Chah-Hwa Chou, Jie-Len Huang, Yu-Kuei Chen, Chun-Chieh Wu
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Publication number: 20130096021Abstract: The present disclosure relates to compositions and methods for cancer diagnosis, research and therapy, including but not limited to, cancer markers. In particular, the present disclosure relates to gene fusions as diagnostic markers and clinical targets for breast cancer.Type: ApplicationFiled: September 27, 2012Publication date: April 18, 2013Inventors: Arul M. Chinnaiyan, Chandan Kumar-Sinha, Dan Robinson, Shanker Kalyana-Sundaram
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Patent number: 8420800Abstract: It is to provide a novel head-and-neck tumor proliferation inhibitor, head-and-neck tumor metastasis inhibitor and pharmaceutical composition for treating a head-and-neck tumor. The present invention is characterized by using an inhibitory substance of a microRNA whose expression increases in a head-and-neck tumor and/or a promoting substance of a microRNA whose expression decreases in a head-and-neck tumor. Preferred examples of the microRNA whose expression increases in a head-and-neck tumor include miR-455-3p, miR-455-5p, miR-130b, miR-130b*, miR-801, miR-196a, miR-21 and miR-31. Preferred examples of the microRNA whose expression decreases in a head-and-neck tumor include miR-133b, miR-145 and miR-375.Type: GrantFiled: April 16, 2010Date of Patent: April 16, 2013Assignee: Keio UniversityInventors: Takeru Zama, Koichiro Saito, Akira Hirasawa, Koji Inagaki
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Patent number: 8420324Abstract: Methods for making cDNA molecules, for amplification of RNA by PCR and for preparation of cDNA libraries are provided. Kits for making cDNA molecules also are provided. Compositions are also provided comprising mixtures of reagents, including reverse transcriptases, buffers, cofactors and other components, suitable for immediate use in conversion of RNA into cDNA and RT PCR without dilution or addition of further components. These compositions are useful, alone or in the form of kits, for cDNA synthesis or nucleic acid amplification (e.g., by the Polymerase Chain Reaction) or for any procedure utilizing reverse transcriptases in a variety of research, medical, diagnostic, forensic and agricultural applications.Type: GrantFiled: December 8, 2009Date of Patent: April 16, 2013Assignee: Quanta BiosciencesInventors: Ayoub Rashtchian, David Schuster
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Publication number: 20130089867Abstract: The present invention is referred to two human nucleic acids that comprise sequences encoding two new isoforms of the human somatostatin receptor type 5 originated by alternative splicing, named sst5B and sst5C, with possible involvement in tumor processes. In addition, the invention is referred to oligonucleotide pairs allowing their differential detection in several tissues using the PCR technique.Type: ApplicationFiled: December 13, 2012Publication date: April 11, 2013Applicant: UNIVERSIDAD DE CORDOBAInventor: Universidad De Cordoba
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Publication number: 20130089861Abstract: The present invention relates to a method, in particular an in vitro method, for identifying certain immune cells of a mammal, comprising analysing the methylation status of at least one CpG position in the gene CCR6 and/or BLR1 or an orthologous or paralogous gene thereof, and the use of DNA-methylation analysis of the genes of the proteins CCR6 and/or BLR1 for a detection and quality assurance and control of certain immune cells. In particular, the present invention relates to analysing the methylation status of at least one CpG position in the gene CCR6 in T cells. Furthermore, the present invention relates to a kit for performing the above methods, as well as to respective uses.Type: ApplicationFiled: April 29, 2011Publication date: April 11, 2013Inventor: Sven Olek
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Publication number: 20130090263Abstract: The invention is directed to polypeptides having a fluorescent activity, e.g., an auto-fluorescent activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used as noninvasive fluorescent markers in living cells and intact organs and animals. The polypeptides of the invention can be used as, e.g., in vivo markers/tracers of gene expression and protein localization, activity indicators, fluorescent resonance energy transfer (FRET) markers, cell lineage markers/tracers, reporters of gene expression and as markers/tracers in protein-protein interactions.Type: ApplicationFiled: November 30, 2012Publication date: April 11, 2013Applicant: BP CORPORATION NORTH AMERICA INC.Inventor: BP CORPORATION NORTH AMERICA INC.
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Patent number: 8415098Abstract: Provided is a method for specifically isolating any genomic region while maintaining interaction of the genomic region with its interacting molecule(s).Type: GrantFiled: August 20, 2010Date of Patent: April 9, 2013Assignee: Osaka UniversityInventors: Hodaka Fujii, Toshitsugu Fujita
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Patent number: 8415097Abstract: The invention provides isolated nucleic acid and amino acid sequences of novel human tumor suppressors, antibodies to such tumor suppressors, methods of detecting such nucleic acids and proteins, methods of screening for modulators of tumor suppressors, and methods of diagnosing and treating tumors with such nucleic acids and proteins.Type: GrantFiled: October 11, 2011Date of Patent: April 9, 2013Assignee: The United States of America as represented by the Secretary of the Department of Health and Human ServicesInventors: Curtis C. Harris, Makoto Nagashima
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Publication number: 20130085081Abstract: Disclosed are a method of detecting the presence or absence of a single nucleotide polymorphism of a gene, for prediction of the risk of developing drug-induced lung injury, or for improving a therapeutic method, and a kit for carrying out the detection method. The detection method is characterized by comparing an ABCB1 gene in a biological sample with a wild-type ABCB1 gene to detect the presence or absence of a single nucleotide polymorphism in the ABCB1 gene in the biological sample, in particular, by determining the nucleotide at position 3751 of the CDS of the ABCB1 gene. The kit comprises an oligonucleotide probe which specifically binds to a single nucleotide polymorphism in an ABCB1 gene under selective binding conditions, or an oligonucleotide primer which amplifies a nucleic acid sequence comprising a single nucleotide polymorphism in an ABCB1 gene.Type: ApplicationFiled: June 8, 2011Publication date: April 4, 2013Applicants: National Cancer Center, Kinki University, Mitsubishi Chemical Medience CorporationInventors: Fumiaki Koizumi, Shintaro Kanda, Tomohide Tamura, Koichi Goto, Masaru Sekijima, Akira Ohide, Kazuto Nishio
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Publication number: 20130084564Abstract: Polynucleotides useful for detecting copy number variation of RNU2 sequences and methods of assessing risk of developing breast or ovarian cancer using molecular combing and/or detection or quantification of BRCA1 expression.Type: ApplicationFiled: June 1, 2012Publication date: April 4, 2013Applicants: Genomic Vision, Centre Leon Berard, Universite Claude Bernard Lyon 1, Centre National de la Recherche ScientifiqueInventors: Sylvie Mazoyer, Chloe Tessereau, Maurizio Ceppi, Kevin Cheeseman
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Patent number: 8409856Abstract: Disclosed herein are compounds, compositions and methods for modulating the expression of a Gemin Gene. Also provided are methods of target validation. Also provided are uses of disclosed compounds and compositions in the manufacture of a medicament for treatment of diseases and disorders.Type: GrantFiled: May 26, 2010Date of Patent: April 2, 2013Assignee: Isis Pharmaceuticals, Inc.Inventors: Kenneth W. Dobie, Susan M. Freier
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Patent number: 8409807Abstract: This invention features systems and methods for the detection of analytes, and their use in the treatment and diagnosis of disease.Type: GrantFiled: February 1, 2012Date of Patent: April 2, 2013Assignee: T2 Biosystems, Inc.Inventors: Lori Anne Neely, Mark John Audeh, Matthew Blanco, James Franklin Chepin, Vasiliki Demas, Rahul K. Dhanda, Thomas Jay Lowery, Jr.
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Patent number: 8409802Abstract: The invention provides, inter alia, novel probes, methods, reaction mixtures, and kits for detecting the presence or absence of a target nucleic acid sequence.Type: GrantFiled: August 9, 2010Date of Patent: April 2, 2013Assignee: Roche Molecular Systems, Inc.Inventor: Stephen G. Will
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Patent number: 8409805Abstract: Methods are provided for increasing the processivity of DNA polymerases on GC-rich templates. The methods relate to providing enhancers and biased ratios of dNTPs, and may be used in DNA amplification reactions. The methods are useful for detecting genotypes associated with GC-rich repeats, including Fragile X Syndrome.Type: GrantFiled: February 13, 2009Date of Patent: April 2, 2013Assignee: Asuragen, Inc.Inventor: Gary J. Latham
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Publication number: 20130079506Abstract: The instant disclosure describes a novel genotype and phenotype assay to elucidate and/or evaluate new potential HIV integrase inhibitors, but also currently approved and experimental compounds that target protease, reverse transcriptase, and RNaseH. This assay allows studying linked mutations and mutational patterns that occur under HAART and experimental therapies.Type: ApplicationFiled: November 5, 2012Publication date: March 28, 2013Applicant: VIRCO BVBAInventor: VIRCO BVBA
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Publication number: 20130078244Abstract: The invention provides for methods for controlling hair growth by administering a HLDGC modulating compound to a subject. The invention further provides for a method for screening compounds that bind to and modulate polypeptides encoded by HLDGC genes. The invention also provides methods of detecting the presence of or a predisposition to a hair-loss disorder in a human subject as well as methods of treating such disorders.Type: ApplicationFiled: July 2, 2012Publication date: March 28, 2013Inventors: ANGELA M. CHRISTIANO, Raphael Clynes
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Patent number: 8404463Abstract: Encoded bead multiplex assays for chromosomal gains and losses are provided that provide the benefits of complex, large template DNA sources, such as BAC DNA, as the probe material without bead networking or other assay performance problems. Reagents for assaying DNA are described herein which include a plurality of encoded particles having attached amplicons amplified from a template DNA sequence. Each individual attached amplicon includes a nucleic acid sequence identical to a random portion of the template DNA sequence, wherein the amplicons together represent substantially the entire template DNA and wherein the nucleic acid sequence identical to a random portion of the template DNA sequence of each individual amplicon is shorter than the entire template DNA.Type: GrantFiled: March 21, 2011Date of Patent: March 26, 2013Assignee: PerkinElmer Health Sciences, Inc.Inventors: Karl Edwin Adler, Jr., Mack J. Schermer
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Patent number: 8404822Abstract: A nucleic acid probe for classification of pathogenic bacterial species is capable of collectively detecting bacterial strains of the same species and differentially detecting them from other bacterial species. Any one of the base sequences of SEQ ID NO. 55 or a combination of at least two of them is used for detecting the gene of an infectious disease pathogenic bacterium.Type: GrantFiled: March 30, 2007Date of Patent: March 26, 2013Assignee: Canon Kabushiki KaishaInventors: Hideto Kuribayashi, Toshifumi Fukui
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Patent number: 8404445Abstract: The present invention features improved in vitro RNA display libraries to allow reliable expression and selection of scFv antibody molecules from expression libraries. The scFv antibody libraries of the invention contain an optimized, shortened inter-domain linker that improves expression scFv antibody expression. The scFv antibody libraries also include short nucleic acid barcodes that allow for identification of individual library clones, libraries or subsets thereof. Primers for generating, amplifying and spectratyping the scFv antibody libraries of the invention are also provided.Type: GrantFiled: September 30, 2009Date of Patent: March 26, 2013Assignee: AbbVie Inc.Inventors: Chung-Ming Hsieh, Yuliya A. Kutskova, John E. Memmott
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Patent number: 8399642Abstract: Provided herein are isolated genomic polynucleotide fragments from the from the p15 region of chromosome 11 encoding human ribosomal protein L26 (RIBO26) and methods of use.Type: GrantFiled: September 24, 2011Date of Patent: March 19, 2013Assignee: Ryogen LLCInventor: James Ryan
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Patent number: 8399639Abstract: Provided herein are isolated genomic polynucleotide fragments from the p15 arm of chromosome 11 encoding HASH2 and methods of use.Type: GrantFiled: September 21, 2011Date of Patent: March 19, 2013Assignee: Ryogen LLCInventor: James Ryan
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Patent number: 8399638Abstract: Provided herein are isolated genomic polynucleotide fragments from the p15 arm of chromosome 11 and methods of use.Type: GrantFiled: September 18, 2011Date of Patent: March 19, 2013Assignee: Ryogen LLCInventor: James Ryan
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Patent number: 8399640Abstract: Provided herein are isolated genomic polynucleotide fragments from the from the p15 region of chromosome 11 encoding human SMS3 (SMS3) and methods of use.Type: GrantFiled: September 21, 2011Date of Patent: March 19, 2013Assignee: Ryogen LLCInventor: James Ryan
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Patent number: 8399197Abstract: The invention provides compositions and methods for amplifying nucleic acid polymer sequences in a high complexity nucleic acid sample. The unique compositions of the invention include a primer set composed of a mixture of two types of primers for DNA synthesis. For extension in one direction, the primers all contain modifications that destroy their ability to serve as templates that can be copied by DNA polymerases. For extension in the opposite direction the set includes at least one primer that can serve as a template and be replicated by DNA polymerases throughout its length. The method can be carried out by mixing the nucleic acid polymer sequence of interest with the set of DNA synthesis primers in an amplification reaction mixture. The reaction mixture is then subjected to temperature cycling analogous to the temperature cycling in PCR reactions. At least one primer in the primer set hybridizes to the nucleic acid polymer.Type: GrantFiled: September 9, 2009Date of Patent: March 19, 2013Assignee: Integrated DNA Technologies, Inc.Inventors: Mark Aaron Behlke, Joseph Alan Walder, Jeffrey A. Manthey
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Patent number: 8399641Abstract: Provided herein are isolated genomic polynucleotide fragments from the from the p15 region of chromosome 11 encoding human tumor suppressing subtransferable candidate 6 and methods of use.Type: GrantFiled: September 24, 2011Date of Patent: March 19, 2013Assignee: Ryogen LLCInventor: James Ryan