Primers Patents (Class 536/24.33)
-
Patent number: 8481292Abstract: Aspects of the present invention include methods and compositions for determining the number of individual polynucleotide molecules originating from the same genomic region of the same original sample that have been sequenced in a particular sequence analysis configuration or process. In these aspects of the invention, a degenerate base region (DBR) is attached to the starting polynucleotide molecules that are subsequently sequenced (e.g., after certain process steps are performed, e.g., amplification and/or enrichment). The number of different DBR sequences present in a sequencing run can be used to determine/estimate the number of different starting polynucleotides that have been sequenced. DBRs can be used to enhance numerous different nucleic acid sequence analysis applications, including allowing higher confidence allele call determinations in genotyping applications.Type: GrantFiled: September 20, 2011Date of Patent: July 9, 2013Assignee: Population Genetics Technologies Litd.Inventors: James Casbon, Sydney Brenner, Robert Osborne, Conrad Lichtenstein, Andreas Claas
-
Patent number: 8481707Abstract: This application describes methods and compositions for detecting and treating vimentin-associated neoplasia. Differential methylation of the vimentin nucleotide sequences has been observed in vimentin-associated neoplasia such as colon neoplasia.Type: GrantFiled: June 27, 2008Date of Patent: July 9, 2013Assignee: Case Western Reserve UniversityInventors: Sanford D. Markowitz, Wei-Dong Chen
-
Patent number: 8481700Abstract: An oligonucleotide, primer or probe comprises the nucleotide sequences of any of SEQ ID NO. 5, 6, 7, 2, 3, 4, 8, 9, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 25. The oligonucleotides are useful for the detection of the methylation status of a gene, in particular the MAGE-A3 gene. The oligonucleotides are useful in primer pairs, kits and methods for determining the methylation status of the MAGE-A3 gene and for diagnosing cancer, directing therapy and selecting subjects for treatment. The primer or probe can comprise a loop or hairpin structure and can be used in real-time methylation specific PCR.Type: GrantFiled: September 17, 2008Date of Patent: July 9, 2013Assignees: MDxHealth SA, GlaxoSmithKline Biologicals SAInventors: Ilse Vlassenbroeck, Katja Bierau
-
Patent number: 8481264Abstract: Provided are methods for sequencing a nucleic acid that include fixing a template to a surface through a template localizing moiety and sequencing the nucleic acid with a sequencing enzyme, e.g. a polymerase or exonuclease. The sequencing enzyme can optionally be exchanged with a second sequencing enzyme, which continues the sequencing of the nucleic acid. The template localizing moiety can optionally anneal with the nucleic acid and/or associate with the sequencing enzyme. Also provided are compositions comprising a nucleic acid fixed to a surface via a template localizing moiety, and a first sequencing enzyme, which can sequence the nucleic acid and optionally exchange with a second sequencing enzyme present in the composition. Compositions in which a template localizing moiety is immobilized on a surface are provided. Compositions for sequencing reactions are provided. Also provided are sequencing systems comprising reaction regions in which or near which template localizing moieties are immobilized.Type: GrantFiled: September 18, 2009Date of Patent: July 9, 2013Assignee: Pacific Biosciences of California, Inc.Inventors: Keith Bjornson, Arek Bibillo, Fred Christians, Kevin Travers, Robin Emig
-
Publication number: 20130172309Abstract: Compositions and methods for the rapid and sensitive detection of a carbapenemase in a sample are provided. The compositions include novel primer and probe compositions for use in detecting the presence of this enzyme in a sample, particularly using PCR methods. These primers and probe sets can be used in amplification methods (such as PCR, particularly quantitative PCR) and packaged into kits for use in amplification methods for the purpose of detecting carbapenemase in a test sample, particularly a patient sample, particularly a direct sample. Thus, in one embodiment, the present invention provides for novel oligonucleotide primers set forth in SEQ ID NOs:1, 2, 4, 5, 7, 8, 14, 15, 17, 18, and 20, and the novel oligonucleotide probe sequences set forth in SEQ ID NOs:3, 6, 9, 16, and 19. These sequences can be used in a method of detecting carbapenemase in a sample.Type: ApplicationFiled: December 3, 2012Publication date: July 4, 2013Applicant: BECTON, DICKINSON AND COMPANYInventor: Becton, Dickinson and Company
-
Publication number: 20130171150Abstract: The invention is directed to a Feline Picorna Virus, an isolated nucleic acid and amino acid sequences therefrom, and uses thereof.Type: ApplicationFiled: July 19, 2012Publication date: July 4, 2013Inventors: Amit Kapoor, Edward J. Dubovi, W. Ian Lipkin
-
Publication number: 20130172206Abstract: The disclosure relates to the genome-wide identification of “rearrangement hotspots”. The disclosure also relates to a microarray chip system for use in detecting genomic rearrangements and a method of manufacturing a microarray chip system useful for detecting genomic rearrangements. The disclosure also relates to methods for detecting genomic rearrangements associated with genetic diseases. The disclosure further relates to methods for using copy number variants in chromosome 2 for detecting Tourette Syndrome.Type: ApplicationFiled: December 14, 2012Publication date: July 4, 2013Inventors: Mohammed Uddin, Proton Rahman, Kathy Hodgkinson, Darren O'Reilly, Sandra Luscombe
-
Patent number: 8476417Abstract: Provided herein are isolated genomic polynucleotide fragments from the from the p15 region of chromosome 11 encoding human and tumor suppressing subtransferable candidate 4 (TSSC4) and methods of use.Type: GrantFiled: September 24, 2011Date of Patent: July 2, 2013Inventor: James Ryan
-
Patent number: 8476063Abstract: Methods and devices for the interfacing of microchips to various types of modules are disclosed. The technology disclosed can be used as sample preparation and analysis systems for various applications, such as DNA sequencing and genotyping, proteomics, pathogen detection, diagnostics and biodefense.Type: GrantFiled: June 15, 2010Date of Patent: July 2, 2013Assignee: IntegenX Inc.Inventors: Stevan Bogdan Jovanovich, Iuliu Ioan Blaga
-
Patent number: 8476044Abstract: A nucleic acid molecule can be annealed to an appropriate immobilized primer. The primer can then be extended and the molecule and the primer can be separated from one another. The extended primer can then be annealed to another immobilized primer and the other primer can be extended. Both extended primers can then be separated from one another and can be used to provided further extended primers. The process can be repeated to provide amplified, immobilized nucleic acid molecules. These can be used for many different purposes, including sequencing, screening, diagnosis, in situ nucleic acid synthesis, monitoring gene expression, nucleic acid fingerprinting, etc.Type: GrantFiled: April 16, 2008Date of Patent: July 2, 2013Assignee: Illumina, Inc.Inventors: Pascal Mayer, Laurent Farinelli, Eric H. Kawashima
-
Publication number: 20130164753Abstract: A method of screening for, diagnosing or detecting the presence of Brachyspira sp. Sask30446 and/or colitis in a subject comprising detecting a Brachyspira sp. Sask30446 polynucleotide or polypeptide in a sample of the subject, wherein detection of the Brachyspira sp. Sask30446 polynucleotide or polypeptide is indicative of the presence Brachyspira sp. Sask30446 and/or that the subject has colitis or has an increased risk of developing colitis.Type: ApplicationFiled: July 18, 2011Publication date: June 27, 2013Inventors: John Clare Samuel Harding, Janet Elizabeth Hill, Manuel Chirino, Joseph Rubin
-
Publication number: 20130164215Abstract: The present invention provides compositions and methods useful for diagnosing, treating, and preventing cancer, particularly ovarian cancer and uterine cancer, based on the discovery that the oocyte specific protein, SAS1R (Sperm Acrosomal SLL-P1 Receptor), which is a sperm protein receptor, is also expressed in various cancers, including ovarian cancer and uterine cancer. Six SAS1R variants have been previously identified, and they are encompassed by the invention. The present invention further provides antibodies useful for targeting SAS1R expressing cells and for killing such cells.Type: ApplicationFiled: August 8, 2011Publication date: June 27, 2013Applicant: University of Virginia Patent Foundation,d/b/a University of Virginia Licensing & VenturesGroupInventors: John C. Herr, Eusebio S. Pires
-
Publication number: 20130164749Abstract: The invention concerns the isolation of nucleotide and peptide sequences in particular for differentiating, in diagnostic terms, an immunisation resulting from BCG vaccination of an infection by M. tuberculosis. Said sequences are either M. bovis BCG/M. bovis specific or M. tuberculosis specific. The invention also concerns a method for detecting said sequences, a method for detecting antibodies generated by the products expressing said sequences as well as kits for implementing said methods. Finally, the invention concerns novel vaccines.Type: ApplicationFiled: December 4, 2012Publication date: June 27, 2013Inventors: Stewart COLE, Stéphen Gordon, Roland Buchrieser-Brosch, Alain Billault, Thierry Garnier
-
Publication number: 20130165334Abstract: A two part assay is disclosed that enables collection of both protein biomarker phenotype and specific HPV genotype data from within a clinically derived population of cervical epithelial cells. Presence of multiple transformation-associated protein biomarkers acts as a gating criterion for cell sorting, followed by application of a PCR protocol sensitive enough to detect and identify individual HPV types from within the cells captured during sorting. The workflow has been optimized to work with cells conventionally fixed in PreservCyt (Cytyc), and it can be performed on residual cells remaining in a stored sample after a Pap test has been performed.Type: ApplicationFiled: March 4, 2011Publication date: June 27, 2013Inventors: Andrew A. Bieberich, Lova N. Rakotomalala, Vincent J. Davisson, Joseph P. Robinson
-
Patent number: 8470533Abstract: This document provides methods and materials for assessing RNA expression. For example, methods and materials for detecting the presence, absence, or amount of target nucleic acid (e.g., target RNA or target cDNA produced from target RNA), kits for detecting the presence, absence, or amount of target nucleic acid (e.g., target RNA or target cDNA produced from target RNA), and methods for making such kits are provided.Type: GrantFiled: February 15, 2011Date of Patent: June 25, 2013Assignee: Cascade Biosystems, Inc.Inventors: Kenneth D. Smith, Nina Yazvenko, Mariya Smit
-
Publication number: 20130157886Abstract: Methods of lung cancer in a sample from a patient are provided. Methods of detecting changes in expression of one or more target RNAs associated with lung cancer are also provided. Compositions and kits are also provided.Type: ApplicationFiled: November 26, 2012Publication date: June 20, 2013Inventors: Bernard MICHOT, Olivier DELFOUR, David H. PERSING
-
Publication number: 20130160155Abstract: The present invention is in the field of plant breeding and aphid resistance. More specifically, the invention includes a method for breeding soybean plants containing quantitative trait loci that are associated with resistance to aphids, Aphis glycines. The invention further includes method for monitoring the introgression quantitative trait loci (QTL) conferring aphid resistance into elite germplasm in a breeding program.Type: ApplicationFiled: February 28, 2013Publication date: June 20, 2013Applicants: UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INC., MONSANTO TECHNOLOGY LLCInventors: MONSANTO TECHNOLOGY LLC, UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INC.
-
Publication number: 20130157873Abstract: Methods of using single nucleotide polymorphisms (SNPs), SNP haplotype block, and haplotype to predict whether or not a subject will develop necrotizing meningoencephalitis (NME) and probe sets that facilitate those methods are disclosed. In particular, the subject is a canine species.Type: ApplicationFiled: May 19, 2011Publication date: June 20, 2013Applicant: TRANSLATIONAL GENOMICS RESEARCH INSTITUTEInventors: Matthew Huentelman, Scott Schatzberg, Renee Barber
-
Patent number: 8465928Abstract: The present invention provides assays for detecting the presence of the PV-BNGT04(RT73) canola event based on the DNA sequence of the recombinant construct inserted into the canola genome and of genomic sequences flanking the insertion site.Type: GrantFiled: April 6, 2011Date of Patent: June 18, 2013Assignee: Monsanto Technology LLCInventors: Rachel Krieb, Qingyi Zeng
-
Patent number: 8466272Abstract: Provided is a probe set that is useful for identifying each allele of HLA individually, and a method of identification of an allele of HLA by the use thereof for each type. The probe set is composed of probes that cover all of the partial sequences that contain a unique base to each allele. Using this probe set HLA contained in a specimen is identified.Type: GrantFiled: May 9, 2012Date of Patent: June 18, 2013Assignee: Canon Kabushiki KaishaInventor: Mamoru Tsukada
-
Patent number: 8465922Abstract: Methods and systems for monitoring reactions by observing signals deriving from those reactions, using signal processing that allows differentiation between signals that are otherwise optically overlapping by conventional detection methods. Centroid determination is used to identify signal sources that are presenting confounding overlapping signals due to their physical proximity, and/or to identify discrete signals from different reaction centers.Type: GrantFiled: August 24, 2011Date of Patent: June 18, 2013Assignee: Pacific Biosciences of California, Inc.Inventors: John Eid, Stephen Turner
-
Patent number: 8465951Abstract: The present invention relates to the diagnostic methods for identification of the single causative agent or more than one causative agent of ocular and nervous system infections among many probable pathogens, which can cause the infection. All the pathogens affecting a discrete area of eye or nervous system generally cause same clinical manifestations or syndromes. The present invention relates to detection and discrimination of the pathogen among the set of probable pathogens in a single test without resorting to a battery of tests each being directed at detection of one pathogen. The current invention aims at the syndrome based diagnostic replacing the diagnostics based on detection of individual pathogens.Type: GrantFiled: May 27, 2008Date of Patent: June 18, 2013Assignee: Council of Scientific & Industrial ResearchInventors: Chintalagiri Mohan Rao, Ikunchala Sridhar Rao, Puppala Venkat Ramchander, Hajib Naraharirao Madhavan, Savitri Sharma, Gita Satpathy, Venkata Banda Ravi Kumar
-
Patent number: 8466274Abstract: Small interfering RNAs (siRNAs) or small hairpin RNA (shRNAs) and compositions comprising same are provided that target human cyclophilin A (CyPA) to inhibit Hepatitis C (HCV) infection. Such siRNA and shRNAs may have a length of from about 19 to about 29 contiguous nucleotides corresponding to a specific region of human cyclophilin A (CyPA) cDNA of from about nucleotide 155 to about nucleotide 183 having particular potency against CyPA and HCV. Such siRNA and shRNAs may be formulated as naked compositions or pharmaceutical compositions. DNA polynucleotides, plasmids, and viral or non-viral vectors are also provided that encode siRNA or shRNA molecules, which may be delivered directly to cells or in combination with delivery agents, such as lipids, polymers, encapsulated lipid particles, such as liposomes. Methods for treating, managing inhibiting, preventing, etc., HCV infection using such siRNA and shRNAs and compositions comprising same are also provided.Type: GrantFiled: October 2, 2012Date of Patent: June 18, 2013Assignee: Florida State University Research FoundationInventor: Hengli Tang
-
Publication number: 20130149702Abstract: The invention provides a method for rapidly detecting a pathogen in fish comprising conducting loop-mediated isothermal amplification with a specific primer set and a nucleic acid in a test sample. If at least one amplification is carried out, the test sample comprises the pathogen in fish. The invention also provides a primer set, probe and kit for detecting a pathogen in fish.Type: ApplicationFiled: December 3, 2012Publication date: June 13, 2013Applicant: NATIONAL CHENG KUNG UNIVERSITYInventor: NATIONAL CHENG KUNG UNIVERSITY
-
Publication number: 20130150426Abstract: Described herein are materials and methods for the diagnosis of idiopathic pulmonary fibrosis.Type: ApplicationFiled: November 21, 2012Publication date: June 13, 2013Applicant: INTERMUNE, INC.Inventor: INTERMUNE, INC.
-
Publication number: 20130149711Abstract: The present invention relates to new BARD1 isoforms specific to lung cancer and colorectal cancer, a method for detecting thereof and a method for treating and/or preventing lung cancer and colorectal cancer.Type: ApplicationFiled: August 17, 2011Publication date: June 13, 2013Applicants: HOPITAUX UNIVERSITAIRES DE GENEVE, UNIVERSITE DE GENEVEInventors: Irmgard Irminger-Finger, Yong-Qiang Zhang
-
Patent number: 8460934Abstract: The present teachings relate to improved methods, kits, and reaction mixtures for amplifying nucleic acids. In some embodiments a novel direct buffer formulation is provided which allows for the direct amplification of the nucleic acids in a crude sample with minimal sample purification.Type: GrantFiled: April 3, 2012Date of Patent: June 11, 2013Assignee: Applied Biosystems, LLCInventors: Chien-Wei Chang, Lori Hennessy, Dennis Wang
-
Patent number: 8460924Abstract: The present invention relates to nucleic acid molecule compositions comprising MiniVectors™ encoding a nucleic acid sequence and methods of gene therapy using MiniVectors encoding a nucleic acid sequence.Type: GrantFiled: October 15, 2010Date of Patent: June 11, 2013Assignee: Baylor College of MedicineInventors: E. Lynn Zechiedrich, Jonathan Fogg, Daniel James Catanese, Jr., Erol Bakkalbasi, Brian E. Gilbert
-
Publication number: 20130143219Abstract: The present invention is directed to methods and compositions for amplifying nucleic acids. Included in the present invention are methods and compositions that amplify nucleic acids with high yield with the formation of unstable target extension products, preferably with minimal or no introduction of allelic bias. Also included in the present invention are high yield, instability primers for use in amplification methods, as multiplexed amplification methods.Type: ApplicationFiled: January 28, 2011Publication date: June 6, 2013Applicant: Medical College of Wisconsin Inc.Inventors: Aoy Tomita Mitchell, Michael Mitchell, Mats Hidestrand
-
Publication number: 20130143203Abstract: The present invention provides compositions, kits and methods for rapid identification and quantification of adventitious contaminant viruses by molecular mass and base composition analysis.Type: ApplicationFiled: May 21, 2012Publication date: June 6, 2013Applicant: IBIS BIOSCIENCES, INC.Inventor: Rangarajan Sampath
-
Patent number: 8455193Abstract: Compositions and methods for nucleic acid sequencing include template constructs that comprise double stranded portions in a partially or completely contiguous constructs, to provide for redundant sequence determination through one or both of sequencing sense and antisense strands, and iteratively sequencing the entire construct multiple times. Additional sequence components are also optionally included within such template constructs. Methods are also provided for the use and preparation of these constructs as well as sequencing compositions for their application.Type: GrantFiled: February 23, 2012Date of Patent: June 4, 2013Assignee: Pacific Biosciences of California, Inc.Inventors: Kevin Travers, Geoff Otto, Stephen Turner, Cheryl Heiner, Congcong Ma
-
Publication number: 20130137669Abstract: The invention relates to a method for determining the responsiveness of a Dirofilaria spp. nematode to a macrocyclic lactone, by determining the genotype of the nematode at a position in a P-glycoprotein gene of the nematode corresponding to position 1 1, and optionally to position 618, in SEQ ID NO: 1, wherein the genotype GG at a position corresponding to position 1 1, or at positions corresponding to positions 1 1 and 618, in SEQ ID NO: 1 indicates that the nematode is likely resistant to the macrocyclic lactone. The invention also relates to methods for selecting a treatment to treat an animal infected with macrocyclic lactone resistant Dirofilaria spp nematode, and treating the animal; or for selecting a prophylactic to prevent an animal from becoming infected with a macrocyclic lactone resistant Dirofilaria spp nematode, and providing the prophylactic to the animal. The invention further relates to an isolated nucleic acid having at least 80% sequence identity to SEQ ID NO: 1.Type: ApplicationFiled: March 30, 2011Publication date: May 30, 2013Inventors: Roger Prichard, Catherine Bourguinat, Timothy Geary, Rudolf Schenker
-
Publication number: 20130137589Abstract: The methods and compositions provided herein relate to the discovery of 13 STR markers, found on the human Y chromosome, having surprisingly high mutation rates when compared with 173 other Y-STR markers known today. The set of RM-Y-STRs may overcome the current dilemma of Y-chromosome analysis in forensic applications due to their extraordinary mutation properties. Embodiments of the invention include methods for allelic determination of rapidly-mutating Y-STR markers, amplification primers for the analysis of rapidly-mutating Y-STR markers, allelic ladders for analysis of rapidly-mutating Y-STR markers, and kits for the analysis of rapidly-mutating Y-STR markers.Type: ApplicationFiled: September 10, 2010Publication date: May 30, 2013Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Rixun FANG, Manohar FURTADO, Manfred KAYSER, Kaye BALLANTYNE
-
Patent number: 8450059Abstract: Provided are quantitative PCR-based compositions and methods for the diagnosis of invasive pulmonary aspergillosis (IPA) in a patient sample, such as bronchoalveolar lavage (BAL) fluid. The methods presented herein involve isolating a patient sample, optionally extracting DNA from the sample, carrying out a quantitative PCR (qPCR) reaction on the sample to generate an amplicon that includes a region of an Aspergillus spp. ribosomal RNA (rRNA) gene, and detecting the PCR amplicon. The present disclosure also provides primers and primer sets for specifically detecting an Aspergillus spp. fungal pathogen in the presence of human ribosomal DNA (rDNA).Type: GrantFiled: April 27, 2010Date of Patent: May 28, 2013Assignee: Fred Hutchinson Cancer Research CenterInventors: David N. Fredricks, Prasanna D. Khot, Daisy L. Ko
-
Patent number: 8450290Abstract: The invention provides the combination use of antisense oligomers targeting androgen receptor mRNA and androgen receptor binding inhibitors that reduce androgen receptor activity for the treatment of androgen receptor related medical disorders, such as cancers, particularly prostate cancers and breast cancers.Type: GrantFiled: November 12, 2010Date of Patent: May 28, 2013Assignees: Enzon Pharmaceuticals, Inc., Santaris Pharma A/SInventors: Jesper Worm, Yixian Zhang
-
Publication number: 20130130239Abstract: The present invention relates to novel tools for detecting Xanthomonas axonopodis pv. allii, in particular the molecular detection of specific polynucleotide sequences of said strain.Type: ApplicationFiled: October 21, 2010Publication date: May 23, 2013Inventors: Isabelle Robene, Olivier Pruvost, Delphine Legrand
-
Publication number: 20130130235Abstract: The present disclosure gives description of a method used for the detection and quantification of dengue viral infection caused by dengue virus using nucleic acids isolated from blood, plasma or serum samples by employing Oligonucleotide probes. The method employed here for detection is by Real time PCR. The instant disclosure also provides for primers, probes, PCR Reaction mixture and kit thereof.Type: ApplicationFiled: July 15, 2011Publication date: May 23, 2013Applicant: BIGTEC PRIVATE LIMITEDInventors: Manjula Jagannath, Manoj Mulakkapurath Narayanan, Chandrasekhar Bhaskaran Nair, Pillarisetti Venkata Subbarao
-
Publication number: 20130129736Abstract: The present invention relates to a method for the in vitro determination of the presence of or a predisposition of a patient to the development of cancer. In the method according to the invention, the presence of a marker is determined in a biological sample of the patient, said marker being selected from a) the amino acid sequence SEQ ID Nr. 2 from the sequence protocol that is provided, or b) a nucleic acid that encodes the amino acid sequence with the SEQ ID Nr. 2. The invention further relates to the amino acid and the encoding nucleic acid and to the use thereof in diagnostics and for molecular therapeutic approaches.Type: ApplicationFiled: January 29, 2013Publication date: May 23, 2013Applicant: Eberhard-Karls-Universit.t Tubingen Universit. tsklinikumInventor: Eberhard-Karls-Universit.t Tubingen Universit.tsklinikum
-
Publication number: 20130130249Abstract: A method for producing a nucleic acid molecule from a template nucleic acid sequence and a linking unit attached to a primer, which method comprises a step of contacting the template nucleic acid sequence with a nucleic acid polymerase under conditions which allow the nucleic acid polymerase to produce the nucleic acid molecule from the primer based on the template nucleic acid sequence, wherein the linking unit is attached to a target site in the template nucleic acid sequence with a covalent linkage.Type: ApplicationFiled: November 16, 2012Publication date: May 23, 2013Applicant: Vilnius UniversityInventor: Vilnius University
-
Patent number: 8445661Abstract: Disclosed is an isolated strain of a previously unknown Staphylococcus, Staphylococcus pseudolugdunensis. Also disclosed are the sequences of the S. pseudolugdunensis tuf gene and 16s rRNA and methods for distinguishing S. pseudolugdunensis from other staphylococcal species.Type: GrantFiled: November 9, 2007Date of Patent: May 21, 2013Assignees: Qiagen Sciences, LLC, Vanderbilt UniversityInventors: Yi-Wei Tang, Charles W. Stratton, Jian Han
-
Patent number: 8445206Abstract: The present disclosure relates to a set of at least 100 single-stranded oligonucleotide probes directed against (or complementary to) portions of a genomic target sequence of interest. The present disclosure also relates to a method of detecting a genomic target sequence of interest using the set of oligonucleotide probes and a method of generating the set of oligonucleotide probes. Further, the present disclosure relates to a kit comprising the set of oligonucleotide probes and at least one further component.Type: GrantFiled: January 30, 2012Date of Patent: May 21, 2013Assignee: Roche Diagnostics Operations, Inc.Inventors: Frank Bergmann, Walter Eberle, Thomas Fischer, Herbert von der Eltz
-
Patent number: 8445454Abstract: Compositions and methods are provided for the treatment of an ischemic cardiovascular condition by providing a patient with a novel non-viral minicircle DNA vector comprising polynucleotide sequences that potentiate HIF-1 activity, including RNAi or antisense agents selective for proteins involved in HIF1 inactivation.Type: GrantFiled: October 22, 2010Date of Patent: May 21, 2013Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Joseph Wu, Mark A. Kay, Mei Huang, Zhi-Ying Chen
-
Patent number: 8445664Abstract: This invention provides novel processes for amplifying nucleic acid sequences of interest, including linear and non-linear amplification. In linear amplification, a single initial primer or nucleic acid construct is utilized to carry out the amplification process. In non-linear amplification, a first initial primer or nucleic acid construct is employed with a subsequent initial primer or nucleic acid construct. In other non-linear amplification processes provided by this invention, a first initial primer or nucleic acid construct is deployed with a second initial primer or nucleic acid construct to amplify the specific nucleic acid sequence of interest and its complement that are provided. A singular primer or a singular nucleic acid construct capable of non-linear amplification can also be used to carry out non-linear amplification in accordance with this invention.Type: GrantFiled: October 20, 2005Date of Patent: May 21, 2013Assignee: Enzo Diagnostics, Inc.Inventors: Elazar Rabbani, Jannis Stavrianopoulos, James J. Donegan, Jack Coleman, Marleen Walner
-
Patent number: 8445201Abstract: A method, device and system for hybridizing a target oligonucleotide to at least one array comprising a plurality of mixing beads are provided. A target solution is mixed by agitating the mixing beads while the target oligonucleotides are hybridizing to the complementary probes on the array. In another embodiment, a permeable barrier contains the mixing beads, thereby preventing them from contacting the array surface.Type: GrantFiled: July 30, 2010Date of Patent: May 21, 2013Assignee: Affymetrix, Inc.Inventors: Bellon Laurent, Martin J. Goldberg, Robert J. Lipshutz, Kaliyur Narasimhan
-
Publication number: 20130122493Abstract: The present invention relates to methods, kits, probes, and systems for distinguishing between nucleotide variants that are close in proximity on a gene. The methods, kits, probes, and systems can include the use of a small amplicon assay in combination with two unlabeled probes in a high resolution thermal melting analysis of a biological sample containing a locus of interest in order to discern between disease-causing and benign variants that are close in proximity on a gene within the biological sample. The present invention also relates to method of detecting a disease in a patient based on the patient's genotype by determining whether the patient has a disease-causing variant at a locus of interest. The signature melt curves produced by the unlabeled probe tests can be analyzed using HRMA software to distinguish between disease-causing and benign variants that are close in proximity on a gene within the biological sample.Type: ApplicationFiled: November 16, 2011Publication date: May 16, 2013Applicant: CANON U.S. LIFE SCIENCES, INC.Inventors: LING XU, RENEE HOWELL
-
Publication number: 20130123119Abstract: Disclosed are novel primers for use in the molecular detection of food-threat agents and food-borne pathogens. The primers may be used in combination for the rapid, high-throughput screening PCR-based techniques to simultaneously detect multiple food safety biothreat agents. The multiplex-detection methods have improved sensitivity and specificity for the detection of multiple high-impact food-borne pathogens simultaneously. Real-time PCR assaying techniques using such primers include microarrays and multiplex single-tube arrays, the latter optionally simultaneously with TaqMan probes.Type: ApplicationFiled: November 15, 2011Publication date: May 16, 2013Applicant: TUSKEGEE UNIVERSITYInventors: Woubit Salah ABDELA, Temesgen SAMUEL-TULORE, Teshome YEHUALAESHET
-
Publication number: 20130122505Abstract: The present teachings describe compositions, methods and kits for detection of one or multiple microorganism contaminants in samples. Some embodiments relate to detecting one or more microorganisms producing virulence factors such as a shiga toxin (stx) or an eae. In some embodiments, compositions, methods and kits can detect and identify individual strains and serotypes of shiga toxin producing microorganisms. Some embodiments describe compositions, methods and kits for detecting STEC microbes. Workflows for multiple microbe detection and identification are also described.Type: ApplicationFiled: August 24, 2012Publication date: May 16, 2013Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Robert TEBBS, Craig Cummings, Pius Brzoska, Sharon Matheny, Catherine O'Connell, Manohar Furtado, Rixun Fang, Olga Petrauskene, Sueh-Ning Liew, Michael Schumaker
-
Publication number: 20130125253Abstract: The invention provides specific transgenic cotton plants, plant material and seeds, characterized in that these products harbor a specific transformation event at a specific location in the cotton genome. Tools are also provided which allow rapid and unequivocal identification of the event in biological samples.Type: ApplicationFiled: October 23, 2012Publication date: May 16, 2013Applicant: Bayer CropScience N.V.Inventors: Linda TROLINDER, Sofie Moens, Veerle Habex, Dimitri Paelinck, Evelien Berghman
-
Patent number: 8440811Abstract: The present invention provides a ligand-nucleic acid nanostructure that promotes cell-cell interaction. Specially, the invention provides a ligand-nucleic acid nanostructure for treating tumor in a mammal. The methods of using and making the composition comprising a ligand-nucleic acid nanostructure are also provided.Type: GrantFiled: October 2, 2009Date of Patent: May 14, 2013Assignee: Arizona Board of Regents, a body corporate acting for and on Behalf of Arizona State UniversityInventors: Yung Chang, Hao Yan
-
Patent number: 8440808Abstract: RpoB gene sequences of various species of Acinetobacter bacteria, and a method of detection by molecular identification of various species of Acinetobacter bacteria using rpoB gene sequences.Type: GrantFiled: March 16, 2006Date of Patent: May 14, 2013Assignees: Universite d'Aix-Marseille, Centre National de la Recherche Scientifique (CNRS)Inventors: Bernard La Scola, Didier Raoult