Primers Patents (Class 536/24.33)
-
Patent number: 8362227Abstract: The invention provides methods to detect C. difficile in biological samples using real-time PCR. Primers and probes for the detection of C. difficile are provided by the invention. Articles of manufacture containing such primers and probes for detecting C. difficile are further provided by the invention.Type: GrantFiled: January 9, 2012Date of Patent: January 29, 2013Assignee: Mayo Foundation for Medical Education and ResearchInventors: Franklin R. Cockerill, III, Thomas F. Smith, Jon E. Rosenblatt, Lynne M. Sloan
-
Patent number: 8361708Abstract: The present invention relates to an isolated novel virus causing Severe Acute Respiratory Syndrome (SARS) in humans (“hSARS virus”). The hSARS virus is identified to be morphologically and phylogenetically similar to known member of Coronaviridae. The present invention provides the complete genomic sequence of the hSARS virus. Furthermore, the invention provides the nucleic acids and peptides encoded by and/or derived from the hSARS virus and their use in diagnostic methods and therapeutic methods, including vaccines. In addition, the invention provides chimeric or recombinant viruses encoded by said nucleotide sequences and antibodies immunospecific to the polypeptides encoded by the nucleotide sequences.Type: GrantFiled: February 10, 2010Date of Patent: January 29, 2013Assignee: Versitech LimitedInventors: Joseph S. M. Peiris, Kwok Yung Yuen, Lit Man Poon, Yi Guan, Kwok Hung Chan, John M. Nicholls, Frederick C. Leung
-
Patent number: 8361710Abstract: The present invention provides novel methods and compositions for the diagnosis, prognosis and treatment of lung cancer. The invention also provides methods of identifying anti-lung cancer agents.Type: GrantFiled: March 30, 2011Date of Patent: January 29, 2013Assignees: The Ohio State University Research Foundation, The United States of America as represented by the Department of Health and Human ServicesInventors: Carlo M. Croce, Nozomu Yanaihara, Curtis C. Harris
-
Publication number: 20130022624Abstract: The present invention discloses humanized anti-epidermal growth factor receptor antibodies, which have favorable binding activity (the binding affinity being 5.09×10?10 mol/L) and are able to inhibit the growth and migration of tumor cells. The present invention also discloses the preparation methods and uses of the antibodies.Type: ApplicationFiled: December 30, 2009Publication date: January 24, 2013Applicant: Guangzhou Recomgen Biotech Co., Ltd.Inventors: David Weaver, Michael Rynkiewicz, Cheng Cao, Yanwen Jin
-
Publication number: 20130022975Abstract: An atherosclerotic disease such as myocardial infarction or angina pectoris is detected by analyzing a single nucleotide polymorphism on human chromosome 5p15.3, and by associating results of the analysis with the risk of the onset thereof. Examples of the single nucleotide polymorphism on human chromosome 5p15.3 include a nucleotide corresponding to the nucleotide at position 61 in the nucleotide sequence of SEQ ID NO: 1, SEQ ID NO: 2, or SEQ ID NO: 3, and a polymorphism at a nucleotide which is in linkage disequilibrium with the above nucleotide.Type: ApplicationFiled: February 18, 2011Publication date: January 24, 2013Applicant: RIKENInventors: Kouichi Ozaki, Toshihiro Tanaka, Yusuke Nakamura
-
Patent number: 8357493Abstract: The invention concerns the isolation of nucleotide and peptide sequences in particular for differentiating, in diagnostic terms, an immunization resulting from BCG vaccination of an infection by M. tuberculosis. Said sequences are either M. bovis BCG/M. bovis specific or M. tuberculosis specific. The invention also concerns a method for detecting said sequences, a method for detecting antibodies generated by the products expressing said sequences as well as kits for implementing said methods. Finally, the invention concerns novel vaccines.Type: GrantFiled: November 4, 2004Date of Patent: January 22, 2013Assignee: Institut PasteurInventors: Stewart Cole, Stéphen Gordon, Roland Buchrieser-Brosch, Alain Billault, Thierry Garnier
-
Publication number: 20130019334Abstract: A novel transgenic corn event designated MZDT09Y is disclosed. The invention relates to nucleic acids that are unique to event MZDT09Y and to methods of detecting the presence of event MZDT09Y based on DNA sequences of the recombinant constructs inserted into the corn genome that resulted in the MZDT09Y event and of genomic sequences flanking the insertion site. The invention further relates to corn plants comprising the transgenic genotype of event MZDT09Y and to methods for producing a corn plant by crossing a corn plant comprising the MZDT09Y genotype with itself or another corn variety. Seeds of corn plants comprising the MZDT09Y genotype are also objects of the invention.Type: ApplicationFiled: July 16, 2012Publication date: January 17, 2013Applicant: SYNGENTA PARTICIPATIONS AGInventors: Rebecca M. CADE, Kateri DUNCAN, Michael L. NUCCIO, Weining GU, YanShan JI, Chunyang FAN, Joseph DIMAIO, Paul ALTENDORF, Wenjin YU
-
Publication number: 20130017221Abstract: The present invention provides an easy and rapid method for detecting/identifying the presence or absence of specific Plasmodium parasites and four species of malaria parasites in a human specimen, an anti-malaria measure support system, and a malaria infection-prevention/treatment system, which can contribute to practical diagnosis in a malaria endemic area. According to the present invention, using a genus-specific primer set that can detect four Plasmodium parasites that infect humans at a time, and the primer sets each specific to each of four species of Plasmodium parasites (P. falciparum, P. vivax, P. malariae, and P. ovale), the presence or absence of infection with these parasites can be detected/identified easily and rapidly.Type: ApplicationFiled: June 13, 2012Publication date: January 17, 2013Applicants: OTSUKA PHARMACEUTICAL CO., LTD., EHIME UNIVERSITYInventors: Takafumi TSUBOI, Eun-Taek HAN
-
Publication number: 20130019342Abstract: The present invention relates generally to the field of molecular biology and regards various polynucleotides, polypeptides and methods that may be employed to enhance yield in transgenic plants. Specifically the transgenic plants may exhibit any one of the traits consisting of increased yield, increased tolerance to abiotic stress, increased cell growth and increased nutrient use efficiency.Type: ApplicationFiled: July 16, 2012Publication date: January 17, 2013Applicant: SYNGENTA PARTICIPATIONS AGInventors: Kateri DUNCAN, Michael L. NUCCIO, Weining GU
-
Publication number: 20130018173Abstract: Antigen-specific immunoglobulin V-regions are identified from a library of nucleic acids amplified using polymerase chain reaction using leader sequence-specific forward primers. The use of leader sequence primers allows all V-region sequences to be amplified (including those with extensive 5? end mutations) without loss of the original 5? V gene segment sequence. A second V-region library is made using a larger than conventional set of 5? V-region primers. The sequence errors introduced into the amplification products by this method are corrected using sequence information obtained in the products amplified by the V-region primers to screen the library created using the leader sequence primers. Amino acid sequence information from fragments of donor immunoglobulins can be used to assist in the identification of nucleic acids encoding the heavy and light chains of donor antibodies as well as to design primers to amplify such nucleic acids.Type: ApplicationFiled: July 12, 2012Publication date: January 17, 2013Applicant: XBIOTECH, INC.Inventor: John Simard
-
Patent number: 8354519Abstract: A novel transgenic corn event designated MIR604, is disclosed. The invention relates to DNA sequences of the recombinant constructs inserted into the corn genome and of genomic sequences flanking the insertion site that resulted in the MIR604 event. The invention further relates to assays for detecting the presence of the DNA sequences of MIR604, to corn plants and corn seeds comprising the genotype of MIR604 and to methods for producing a corn plant by crossing a corn plant comprising the MIR604 genotype with itself or another corn variety.Type: GrantFiled: January 19, 2011Date of Patent: January 15, 2013Assignee: Syngenta Participations AGInventors: Henry-York Steiner, Eric Chen, Moez Meghji
-
Patent number: 8354252Abstract: Labeled reactant compositions, and particularly labeled nucleic acid reaction compositions, that include structural components that maintain potentially damaging labeling components sufficiently distal from the reactant portion of the molecule such that damaging effects of the label group on other reaction components, such as enzymes, are reduced, minimized and/or eliminated.Type: GrantFiled: May 25, 2011Date of Patent: January 15, 2013Assignee: Pacific Biosciences of California, Inc.Inventors: Jeffrey Wegener, Jonas Korlach
-
Publication number: 20130011831Abstract: The present application describes compositions and methods useful for the rapid detection of Legionella pneumophila. The compositions include capture probes, amplification primers, primer sets and detection probes that comprise nucleic acid molecules that hybridize to L. pneumophila 23S rRNA or DNA encoding 23S rRNA target sequences. Also described are methods for detecting and/or quantifying the amount of L. pneumophila in a sample using real time PCR (rPCR) or revere transcriptase real time PCR (RT-rPCR).Type: ApplicationFiled: March 25, 2010Publication date: January 10, 2013Inventors: Jing Luo, Hong Cai, Jing Chen
-
Patent number: 8349607Abstract: Methods are disclosed for screening for the occurrence of gene silencing (e.g., post transcriptional gene silencing) in an organism. Also provided are methods for isolating silencing agents so identified.Type: GrantFiled: July 23, 2009Date of Patent: January 8, 2013Assignee: Plant Bioscience LimitedInventors: David Charles Baulcombe, Andrew John Hamilton
-
Patent number: 8349566Abstract: Provided herein are optimized methods for performing multiplexed detection of a plurality of sequence variations. Also provided are methods for performing multiplexed amplification of target nucleic acid.Type: GrantFiled: July 28, 2011Date of Patent: January 8, 2013Assignee: Sequenom, Inc.Inventors: Martin Beaulieu, Dirk Johannes Van Den Boom
-
Publication number: 20130005607Abstract: The invention relates to the field of quality testing of fresh plant-based and mushroom based products. Methods, carriers and kits for determining the quality stage are provided.Type: ApplicationFiled: September 11, 2012Publication date: January 3, 2013Applicant: Nsure Holding B.V.Inventors: Monique Francisca VAN WORDRAGEN, Rudolf Aart De Maagd, Jurriaan Johannes Mes, Peter Albert Balk
-
Patent number: 8344117Abstract: A circular DNA is provided comprising endogenous DNA common to both genetically modified wheat and non-genetically modified wheat along with one or more pieces of DNA each having a sequence present specifically in a strain of genetically modified wheat. Also provided is a method for determining a mix rate of genetically modified wheat in a test sample.Type: GrantFiled: August 25, 2011Date of Patent: January 1, 2013Assignees: Nisshin Seifun Group Inc., Incorporated Administrative Agency National Agriculture and Food Research OrganizationInventors: Akihiro Hino, Takashi Kodama, Mayu Iida, Hirohito Yamakawa, Satomi Nozaki, Katsuyuki Hayakawa
-
Patent number: 8344124Abstract: A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 2 and mutated sequences thereof.Type: GrantFiled: April 23, 2012Date of Patent: January 1, 2013Assignee: Canon Kabushiki KaishaInventors: Nobuhiro Tomatsu, Toshifumi Fukui, Nobuyoshi Shimizu, Atsushi Takayanagi
-
Patent number: 8344122Abstract: Mainly provided is a technique for directly identifying the genotype at locus Rf17 based on the specific base sequence data thereof. Also provided is a technique for artificially constructing a fertility-restored line. A method of restoring the fertility of CW-type cytoplasmic male sterile rice by inhibiting or reducing the expression of a gene comprising the base sequence represented by SEQ ID NO:2 in the above-described rice, and a method for determining the presence or absence of gene Rf17, which is a fertility restorer gene for CW-type cytoplasmic male sterility, comprising identifying a single nucleotide polymorphism (SNP) in the base at the 1812 position of the base sequence represented by SEQ ID NO:1 in the rice to be examined.Type: GrantFiled: February 21, 2009Date of Patent: January 1, 2013Assignee: Tohoku UniversityInventors: Kinya Toriyama, Sota Fujii
-
Patent number: 8344123Abstract: An embodiment of a method for detecting low frequency occurrence of one or more HIV sequence variants associated with drug resistance is described that comprises the steps of: generating cDNA species from each RNA molecule in an HIV sample population; amplifying at least one first amplicon from the cDNA species, wherein each first amplicon comprises a plurality of amplified copies and is amplified with a pair of nucleic acid primers that define a locus of the first amplicon; clonally amplifying the amplified copies of the first amplicons to produce a plurality of second amplicons wherein a plurality of the second amplicons comprise an immobilized population of substantially identical copies from one of the amplified copies of first amplicons; determining a nucleic acid sequence composition of the substantially identical copies from at least 100 of the immobilized populations in parallel on a single substrate; and detecting one or more sequence variants that occur at a frequency of 5% or less in the nucleic acType: GrantFiled: December 16, 2010Date of Patent: January 1, 2013Assignee: 454 Life Sciences CorporationInventors: Birgitte Binderup Simen, Elizabeth Patricia St. John
-
Patent number: 8343722Abstract: Disclosed is a method for the early diagnosis of colorectal carcinoma and determination of pre-cancerous lesions of the colon and rectum based on quantitation of DNA extracted from stool and amplified by PCR techniques.Type: GrantFiled: February 27, 2004Date of Patent: January 1, 2013Assignee: Istituto Oncologico Romagnolo Cooperativa Sociale a.r.l.Inventors: Daniele Calistri, Claudia Rengucci
-
Publication number: 20120329726Abstract: A genetic polymorphism and a hair shape susceptibility gene that are related to hair shape, and a method for determining the genetic susceptibility to hair shape in individual test subjects are provided. Disclosed is a hair shape susceptibility gene, which overlaps with a haplotype block in 1q21.3 region (D1S2696 to D1S2346) of human chromosome 1 and comprises a portion or the entirety of the base sequence of the haplotype block, wherein the haplotype block is determined by a linkage disequilibrium analysis conducted on a single nucleotide polymorphism (SNP) marker whose allele frequency differs statistically significantly between a group having a curly hair trait and a group having a non-curly hair trait, and consists of a base sequence set forth in any one of SEQ ID NO: 1 to NO: 5.Type: ApplicationFiled: October 5, 2010Publication date: December 27, 2012Applicant: Kao CorporationInventors: Hiroyuki Taguchi, Hiroshi Yoshida, Chie Fuse, Tadao Arinami
-
Publication number: 20120329041Abstract: Disclosed are compositions including primers and probes, which are capable of interacting with the disclosed nucleic acids, such as the nucleic acids encoding the reverse transcriptase or protease of HIV as disclosed herein. Thus, provided is an oligonucleotide comprising any one of the nucleotide sequences set for in SEQ ID NOS:1-89, and 96-104. Also provided are the oligonucleotides consisting of the nucleotides as set forth in SEQ ID NOS:1-89, and 96-104. Each of the disclosed oligonucleotides is a probe or a primer. Also provided are mixtures of primers and probes and for use in RT-PCR and primary PCR reactions disclosed herein. Provided are methods for the specific detection of several mutations in HIV. Mutations in both the reverse transcriptase and the protease of HIV can be detected using the methods described herein.Type: ApplicationFiled: September 4, 2012Publication date: December 27, 2012Applicant: Centers for Disease Control and PreventionInventors: Jeffrey A. Johnson, Walid M. Heneine
-
Publication number: 20120329055Abstract: The present invention relates to a marker composition for pregnancy diagnosis in cattle, a pregnancy diagnosis composition, and a pregnancy diagnosis method, which use a pregnancy-specific protein in cattle. The present inventors discovered an ?1G subunit protein of the T-type calcium channel expressed specifically in pregnant cows, and produced a specific antibody against this protein. Therefore, the present invention has the effect of detecting pregnancy in cows easily, quickly, and accurately early in the pregnancy.Type: ApplicationFiled: November 7, 2011Publication date: December 27, 2012Inventors: Dong Il Jin, Rong Xun Han, Hong Rye Kim
-
Publication number: 20120329059Abstract: A method for producing bacteria belonging to the genus Bifidobacterium having excellent viability even under various conditions with different environmental factors, novel bacteria belonging to the genus Bifidobacterium obtained by the method, and a method for detecting the bacteria are provided. By subculturing and storing bacteria belonging to the genus Bifidobacterium alternately in systems under conditions with different environmental factors, the bacteria belonging to the genus Bifidobacterium exhibiting excellent viability under all the conditions used for the alternate subculturing and storing can be produced.Type: ApplicationFiled: February 21, 2011Publication date: December 27, 2012Applicant: KABUSHIKI KAISHA YAKULT HONSHAInventors: Tomoyuki Sako, Mika Miura, Yasuhisa Shimakawa, Koji Miyazaki, Junji Fujimoto, Koichi Watanabe
-
Publication number: 20120329052Abstract: The present invention relates to a nucleic acid molecule comprising a 5? portion of an intestinal lactase-phlorizine hydrolase (LPH) gene contributing to or indicative of the adult-type hypolactasia. The present invention further relates to methods for testing for the presence of or predisposition to adult-type hypolactasia that are based on the analysis of an SNP contained in the above recited nucleic acid molecule. Additionally, the present invention relates to diagnostic composition and kit useful in the detection of the presence of or predisposition to adult-type hypolactasia.Type: ApplicationFiled: August 24, 2012Publication date: December 27, 2012Applicant: National Public Health InstituteInventors: Leena Peltonen, Nabil Enattah, Irma Järvelä, Timo Sahi, Erkki Savilahti, Joseph Terwilliger
-
Patent number: 8338099Abstract: The present invention relates to reference genes, primers, and probes for the normalization of gene expression analysis data from blood samples of a patient. The invention further relates to a method for the normalization of gene expression analysis data with the aid of reference genes, primers, or probes.Type: GrantFiled: February 28, 2008Date of Patent: December 25, 2012Assignee: SIRS-Lab GmbHInventors: Stefan Russwurm, Hans-Peter Saluz, Hans-Peter Deigner
-
Publication number: 20120322063Abstract: The present invention is directed to methods, reagents, kits and compositions for identifying and quantifying microRNA (miRNA) precursor expression in a biological sample. The method uses gene-specific primers and reverse transcriptase to convert the primary miRNA precursors (pri-miRNA) and pre-miRNA precursors (pre-miRNAs) to cDNA. The method also uses amplification reactions using gene specific forward and reverse primers that are targeted to the hairpin sequence of pri- and pre-microRNA precursors to detect the expression levels of both the pri- and the pre-micoRNAs. In one embodiment, the amplification reaction is a real-time PCR wherein the level of PCR amplification products produced is related to the levels of the microRNA precursors in the biological sample. In another embodiment, a probe is used to distinguish between similar isoforms of microRNA precursors.Type: ApplicationFiled: May 1, 2012Publication date: December 20, 2012Applicant: THE OHIO STATE UNIVERSITY RESEARCH FOUNDATIONInventor: Thomas D. Schmittigen
-
Publication number: 20120322051Abstract: The invention provides novel methods and compositions for amplifying portions of the HCV genome. The nucleic acid sequences set forth as SEQ ID NOS:1-64 derived from HCV cDNA and functional equivalents thereof, kits containing same, and methods employing same, are useful for the identification and characterization of HCV in biological samples.Type: ApplicationFiled: April 26, 2012Publication date: December 20, 2012Inventor: Carol Holland-Staley
-
Publication number: 20120322667Abstract: The present invention relates, in general, to perioperative bleeding and, in particular, to methods of identifying individuals at risk of perioperative bleeding. The present invention relates, in general, to perioperative renal dysfunction and, in particular, to methods of identifying individuals at risk of perioperative renal dysfunction. The present invention relates, in general, to perioperative stroke and, in particular, to methods of identifying individuals at risk of perioperative stroke.Type: ApplicationFiled: April 24, 2012Publication date: December 20, 2012Applicant: DUKE UNIVERSITYInventors: Debra A. Schwinn, Ian Welsby, Mihai V. Podgoreanu, Mark Stafford-Smith, Mark F. Newman, Hilary P. Grocott, William D. White, Richard W. Morris, Joseph P. Mathew
-
Publication number: 20120322676Abstract: Disclosed are genomic sequences for nine strains of Cronobacter spp. (C. sakazakii—696, 701, 680; C. malonaticus—507, 681; C. turicensis—564; C. muytjensii—530; C. dublinensis—582; C. genomosp1—581) and compositions, methods, and kits for detecting, identifying and distinguishing Cronobacter spp. strains from each other and from non-Cronobacter spp. strains. Some embodiments describe isolated nucleic acid compositions unique to certain Cronobacter strains as well as compositions that are specific to all Cronobacter spp. Primer and probe compositions and methods of use of primers and probes are also provided. Kits for identification of Cronobacter spp. are also described. Some embodiments relate to computer software methods for setting a control based threshold for analysis of PCR data.Type: ApplicationFiled: June 13, 2012Publication date: December 20, 2012Applicant: Life Technologies CorporationInventors: Yongmei JI, Sheung-Mei ("Rita") Shih, Lovorka Degoricija, Zhaohui Zhou, Craig Cummings, Manohar Furtado, Pius Brzoska, Angela Burrell, Harrison Leong, Xingwang Fang, Mangkey Bounpheng, Rohan Shah
-
Publication number: 20120322684Abstract: Disclosed are a method, a composition, a microarray, an antibody and a kit for diagnosis and prognosis of cancer, based on detection of deletion of the exon 3 region of G-CSF gene or levels of a mutated G-CSF protein having a deletion of an amino acid sequence corresponding to the exon 3 region, wherein the deletion of the exon 3 region of the G-CSF gene is used as a cancer biomarker.Type: ApplicationFiled: July 11, 2012Publication date: December 20, 2012Inventors: Sang Yup Lee, Nae Choon Yoo, So Young Yoo, Hyun Cheol Chung, Ki Chang Keum, Won Min Yoo, Ki Jun Jeong
-
Patent number: 8334099Abstract: The present invention provides a method for amplification of a target nucleic acid sequence or signal, wherein an amplification reaction mixture is used which contains at least one reversibly modified oligonucleotide having a non-hydroxyl group 3? end which can be converted into a hydroxyl 3? end upon exposure to a chemical and/or irradiation and/or a range of temperature. The present invention also provides a reversibly modified oligonucleotide as described above, and a nucleic acid amplification reaction mixture and kit comprising such an oligonucleotide.Type: GrantFiled: July 30, 2007Date of Patent: December 18, 2012Inventor: Wanli Bi
-
Patent number: 8334098Abstract: Nucleic acid oligonucleotide sequences are disclosed which include amplification oligomers and probe oligomers which are useful for detecting multiple types of human papillomaviruses (HPV) associated with cervical cancer. Methods for detecting multiple HPV types in biological specimens by amplifying HPV nucleic acid sequences in vitro and detecting the amplified products are disclosed.Type: GrantFiled: August 23, 2011Date of Patent: December 18, 2012Assignee: Gen-Probe IncorporatedInventors: Sylvia A. Norman, Jennifer J. Bungo, William L. Hanna, Neeraj P. Rao
-
Publication number: 20120315642Abstract: A primer and method for amplification of a target nucleic acid, the primer adapted to conform into a conformation that dissociates from a complementary strand of DNA duplex. The conformation may have a free energy with more favorable thermodynamics than a corresponding DNA duplex, such as a B-DNA duplex. The dissociation may occur during an extension step of an amplification method, such as polymerase chain reaction. The method can proceed isothermally, and the primers may include intrinsic fluorescence.Type: ApplicationFiled: February 18, 2011Publication date: December 13, 2012Applicant: THE OHIO STATE UNIVERSITYInventor: Besik Kankia
-
Patent number: 8329406Abstract: Provided is a method whereby walnut can be simply, rapidly and accurately separated and distinguished from pecan nut. The method involves a PCR method for specifically detecting nuts of the family Juglandaceae and identifying the matK sequence of pecan nut to thereby discriminate walnut and pecan nut from each other optionally using restriction enzymes.Type: GrantFiled: January 30, 2008Date of Patent: December 11, 2012Assignees: Oriental Yeast Co., Ltd., Nissin Foods Holdings Co., Ltd.Inventors: Takeo Yano, Yumiko Sakai, Kouji Uchida, Yoshiki Nakao, Kimie Ishihata, Shigeru Nakano, Toshihiro Yamada
-
Publication number: 20120308993Abstract: There is provided a method of detecting the presence of oseltamivir-resistant Influenza A virus in a sample, the method comprising detecting the presence of an amplicon, a fragment and/or a derivative thereof, wherein the amplicon, fragment and/or derivative thereof (a) is amplified by a primer (SEQ ID NO:1), a fragment, or derivative therefore and/or a primer (SEQ ID NO:2), a fragment, or derivative thereof. There are also provided primer(s) and/or probe as well as kit for the detection of the presence of Influenza A in a sample and/or in a subject.Type: ApplicationFiled: November 8, 2010Publication date: December 6, 2012Applicants: TAN TOCK SENG HOSPITAL, AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCHInventors: Masafumi Inoue, Timothy Barkham
-
Publication number: 20120309003Abstract: Disclosed are compositions, methods and kits for the specific detection of enterohemorrahagic E. coli O104:H4 from contaminated samples including clinical samples, biological samples, food samples, complex food matrices, water, beverage samples, fermentation broth samples, forensic samples, environmental samples (e.g., soil, dirt, garbage, sewage, air, or water) as well as food processing and manufacturing surfaces.Type: ApplicationFiled: June 1, 2012Publication date: December 6, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: CRAIG CUMMINGS, PIUS BRZOSKA, ALAIN RICO, YONGMEI ("ALEXIS") JI
-
Publication number: 20120309012Abstract: The present invention relates to group specific primers for direct 16S rDNA sequencing of polybacterial samples.Type: ApplicationFiled: November 12, 2010Publication date: December 6, 2012Applicant: ISENTIO ASInventor: Øyvind Kommedal
-
Publication number: 20120304940Abstract: The present invention relates to methods of genotyping for selecting an animal with a desired trait such as the level monounsaturated fats in muscle tissue, the types and/or ratio of different monounsaturated fats in muscle tissue, marbling, carcass weight, meat quality, speed of finishing, feedlot efficiency and/or consumer preference. In particular the invention relates to methods of selecting an animal with a desired trait by analysing the M-RIP, NT5M and/or TCAP genes for one or more polymorphisms.Type: ApplicationFiled: September 17, 2010Publication date: December 6, 2012Inventors: Roger Letts Dawkins, John Anthony Millman, Joseph Frederick Williamson, Patrick Robert Carnegie
-
Patent number: 8323890Abstract: There is disclosed an improved high-throughput and quantitative process for determining methylation patterns in genomic DNA samples based on amplifying modified nucleic acid, and detecting methylated nucleic acid based on amplification-dependent displacement of specifically annealed hybridization probes. Specifically, the inventive process provides for treating genomic DNA samples with sodium bisulfite to create methylation-dependent sequence differences, followed by detection with fluorescence-based quantitative PCR techniques. The process is particularly well suited for the rapid analysis of a large number of nucleic acid samples, such as those from collections of tumor tissues.Type: GrantFiled: June 2, 2009Date of Patent: December 4, 2012Assignee: The University of Southern CaliforniaInventors: Peter W. Laird, Cindy A. Carroll, Kathleen D. Danenberg
-
Patent number: 8323975Abstract: Telomere-encoding nucleic acid nanocircles, methods for their preparation, and methods for their use are disclosed. The nanocircles can be constructed containing multiple repeats of the complement of telomere repeat sequences. The telomere-encoding nanocircles are useful for extending telomeres both in vitro and in vivo, for treating macular degeneration, the effects of skin aging, liver degeneration, and cancer. The nanocircles are further useful for treating cell cultures to produce long-lived non-cancerous cell populations. This use has wide applicability in scientific research, tissue engineering, and transplantation.Type: GrantFiled: January 26, 2009Date of Patent: December 4, 2012Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventor: Eric T. Kool
-
Patent number: 8323929Abstract: The invention employs an unlabeled signal primer comprising a 5? adapter sequence for detection of variations in nucleic acid target sequences. The detection system further comprises a reporter probe, the 3? end of which hybridizes to the complement of the 5? adapter sequence of the signal primer to produce a 5? overhang. Polymerase is used to fill in the overhang and synthesize the complement of the 5? overhang of the reporter probe. Synthesis of the reporter probe complement is detected, either directly or indirectly, as an indication of the presence of the target.Type: GrantFiled: April 7, 2009Date of Patent: December 4, 2012Assignee: Becton, Dickinson and CompanyInventors: Sha-Sha Wang, Keith Thornton, James G. Nadeau, Tobin J. Hellyer
-
Patent number: 8323896Abstract: The present invention relates to diagnostic and prognostic methods to determine the likelihood of a subject who has a inflammatory disease or liver disease of developing cancer. In particular, the present invention relates to methods for identifying subjects with increased susceptibility to developing cancer, such as hepatocellular carcinoma (HCC) where the subject has an inflammatory disease, such as, but not limited to cirrhosis, by identifying a variance or polymorphism in the human EGF gene. In particular, the methods of the present invention relate to identifying subjects with increased susceptibility to developing cancer such as HCC, where the subject has an inflammatory disease, such as but not limited to cirrhosis, and the subject is identified to have a single nucleotide polymorphism 61A>G in the 5?UTR of the EGF gene.Type: GrantFiled: March 28, 2008Date of Patent: December 4, 2012Assignee: The General Hospital CorporationInventors: Kenneth K Tanabe, Bryan C Fuchs, Hiroshi Kawasaki
-
Patent number: 8324366Abstract: This invention relates to new compositions comprising at least one of a single or double stranded oligonucleotide, where said oligonucleotide has been conjugated to a lipophile and to which the conjugated oligonucleotide has been preassembled with lipoproteins. These compositions are effectively in delivering oligonucleotides to mammalian tissue where they effect gene silencing.Type: GrantFiled: April 29, 2009Date of Patent: December 4, 2012Assignees: Alnylam Pharmaceuticals, Inc., Eth ZurichInventors: Akin Akinc, Tomoko Nakayama, Muthiah Manoharan, Markus Stoffel
-
Patent number: 8324364Abstract: Compositions and methods for the rapid and sensitive detection of a carbapenemase in a sample are provided. The compositions include novel primer and probe compositions for use in detecting the presence of this enzyme in a sample, particularly using PCR methods. These primers and probe sets can be used in amplification methods (such as PCR, particularly quantitative PCR) and packaged into kits for use in amplification methods for the purpose of detecting carbapenemase in a test sample, particularly a patient sample, particularly a direct sample. Thus, in one embodiment, the present invention provides for novel oligonucleotide primers set forth in SEQ ID NOs:1, 2, 4, 5, 7, 8, 14, 15, 17, 18, and 20, and the novel oligonucleotide probe sequences set forth in SEQ ID NOs:3, 6, 9, 16, and 19. These sequences can be used in a method of detecting carbapenemase in a sample.Type: GrantFiled: May 16, 2011Date of Patent: December 4, 2012Assignee: Becton, Dickinson and CompanyInventors: Craig C. Whiteford, Charles Yu
-
Publication number: 20120304330Abstract: The invention provides specific transgenic soybean plants, plant material and seeds, characterized in that these products harbor a specific herbicide tolerance transformation event at a specific location in the soybean genome. Tools are also provided which allow rapid and unequivocal identification of the event in biological samples.Type: ApplicationFiled: November 23, 2010Publication date: November 29, 2012Applicants: M.S. TECHNOLOGIES LLC, BAYER BIOSCIENCE N.V.Inventors: Justin Thomas Mason, Leslie James Lettow, Mark Alan Eby, William H. Eby, Günter Welz, Steven Verhaeghe, Marc De Beuckeleer, Veerle Habex, Jean-Marc Ferrulo
-
Publication number: 20120301874Abstract: The present invention relates to methods for the rapid detection of the presence or absence of Staphylococcus aureus in a biological or nonbiological sample. The present invention includes methods of detection comprising performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, the present invention relates to primers, probes, and kits that are designed for the detection of Staphylococcus aureus.Type: ApplicationFiled: May 26, 2011Publication date: November 29, 2012Applicant: ROCHE MOLECULAR SYSTEMS, INC.Inventor: Jenny A. Johnson
-
Publication number: 20120302741Abstract: Blood plasma of pregnant women contains fetal and (generally >90%) maternal circulatory extracellular DNA. Most of said fetal DNA contains ?500 base pairs, said maternal DNA having a greater size. Separation of circulatory extracellular DNA of <500 base pairs results in separation of fetal from maternal DNA. A fraction of a blood plasma or serum sample of a pregnant woman containing, due to size separation (e.g. by chromatography, density gradient centrifugation or nanotechnological methods), extracellular DNA substantially comprising ?500 base pairs is useful for non-invasive detection of fetal genetic traits (including the fetal RhD gene in pregnancies at risk for HDN; fetal Y chromosome-specific sequences in pregnancies at risk for X chromosome-linked disorders; chromosomal aberrations; hereditary Mendelian genetic disorders and corresponding genetic markers; and traits decisive for paternity determination) by e.g. PCR, ligand chain reaction or probe hybridization techniques, or nucleic acid arrays.Type: ApplicationFiled: July 24, 2012Publication date: November 29, 2012Applicant: SEQUENOM, INC.Inventors: Sinuhe HAHN, Wolfgang Holzgreve, Bernhard Zimmermann, Ying Li
-
Patent number: 8318925Abstract: Small interfering RNAs (siRNAs) or small hairpin RNA (shRNAs) and compositions comprising same are provided that specifically target human cyclophilin A (CyPA) to effectively inhibit Hepatitis C (HCV) infection in a cell. Such siRNA and shRNAs may have a length of from about 19 to about 29 contiguous nucleotides corresponding to a specific region of human cyclophilin A (CyPA) cDNA of from about nucleotide 155 to about nucleotide 183 having particular potency against CyPA and HCV. Such siRNA and shRNAs may be formulated as naked compositions or as pharmaceutical compositions. DNA polynucleotides, plasmids, and viral or non-viral vectors are also provided that encode siRNA or shRNA molecules, which may be delivered directly to cells or in combination with known delivery agents, such as lipids, polymers, encapsulated lipid particles, such as liposomes. Methods for treating, managing inhibiting, preventing, etc., HCV infection using such siRNA and shRNAs and compositions comprising same are also provided.Type: GrantFiled: February 25, 2011Date of Patent: November 27, 2012Assignee: Florida State University Research FoundationInventor: Hengli Tang