Labelled (e.g., Tagged With Radioactive Tracer, Fluorescent Marker, Intercalator, Etc.) Patents (Class 536/26.6)
  • Patent number: 10301349
    Abstract: The present invention provides a family of dark quenchers, termed Black Hole Quenchers (“BHQs”), that are efficient quenchers of excited state energy but which are themselves substantially non-fluorescent. Also provided are methods of using the BHQs, probes incorporating the BHQs and methods of using the probes.
    Type: Grant
    Filed: March 30, 2015
    Date of Patent: May 28, 2019
    Assignee: BIOSEARCH TECHNOLOGIES, INC.
    Inventors: Ronald M. Cook, Matt Lyttle, Daren Dick
  • Patent number: 10156572
    Abstract: Embodiments of the present disclosure provide bisbiotin ligands and related conjugates and methods. The bisbiotin ligands, combined with streptavidin, can be used in the separation, labelling, targeting, and immobilization of biomolecules.
    Type: Grant
    Filed: February 18, 2015
    Date of Patent: December 18, 2018
    Assignee: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
    Inventors: Peiming Zhang, Stuart Lindsay, Subhadip Senapati, Sudipta Biswas
  • Patent number: 9890379
    Abstract: The current invention provides for methods and medicaments that apply oligonucleotide molecules complementary only to a repetitive sequence in a human gene transcript, for the manufacture of a medicament for the diagnosis, treatment or prevention of a cis-element repeat instability associated genetic disorders in humans. The invention hence provides a method of treatment for cis-element repeat instability associated genetic disorders. The invention also pertains to modified oligonucleotides which can be applied in method of the invention to prevent the accumulation and/or translation of repeat expanded transcripts in cells.
    Type: Grant
    Filed: July 27, 2015
    Date of Patent: February 13, 2018
    Assignee: BioMarin Technologies B.V.
    Inventors: Josephus Johannes De Kimpe, Gerard Johannes Platenburg, Derick Gert Wansink
  • Patent number: 9885081
    Abstract: The present invention relates to a novel method for detection of target nucleic acid sequences on a solid phase using dual-labeled immobilized probes and its resistance to a 5? to 3? exonuclease activity of a DNA polymerase. Because the label is remained on the solid substrate by resistance to nucleases due to labeling of a base component the internal nucleotide, the present invention requires no consideration of a suitability of position of the label for remaining on the solid substrate. The present invention ensures to minimize background signal by positioning labels at a site on probes suitable to maximize quenching efficiency of the dual label system, since it permits to freely determine the position of the internal label on probes.
    Type: Grant
    Filed: October 20, 2011
    Date of Patent: February 6, 2018
    Assignee: Seegene, Inc.
    Inventor: Jong Yoon Chun
  • Patent number: 9884889
    Abstract: Provided are compounds comprising two DNA supramolecular binding molecules covalently joined by a linker group. Also provided are multisignal labeling reagents comprising (i) an oligomer of nucleotides or nucleotide analogs; (ii) a DNA supramolecular binding molecule noncovalently bound to the oligomer; and (iii) a first reactive group or a first partner of a first binding pair covalently bound to the oligomer. Additionally provided are methods of producing multisignal labeling reagents.
    Type: Grant
    Filed: September 3, 2015
    Date of Patent: February 6, 2018
    Assignee: Enzo Life Sciences, Inc.
    Inventors: Jack Coleman, Elazar Rabbani, Jannis Stavrianopoulos, Praveen Pande
  • Patent number: 9745336
    Abstract: The present teachings generally relate to fluorescent dyes, linkable forms of fluorescent dyes, energy transfer dyes, reagents labeled with fluorescent dyes and uses thereof.
    Type: Grant
    Filed: March 28, 2014
    Date of Patent: August 29, 2017
    Assignee: Applied Biosystems, LLC
    Inventors: Ronald Graham, Ruiming Zou, Krishna Upadhya, Scott Benson
  • Patent number: 9580749
    Abstract: Dimeric and trimeric nucleic acid dyes, and associated systems and methods are provided. Such a dye may form a hairpin-like structure that enables it to stain nucleic acids via a release-on-demand mechanism, for example. Such a dye may have low background fluorescence in the absence of nucleic acids and high fluorescence in the presence of nucleic acids, upon binding therewith, for example. A dye provided herein may be useful in a variety of applications, such as in DNA quantitation in real-time PCR, for example.
    Type: Grant
    Filed: August 15, 2013
    Date of Patent: February 28, 2017
    Assignee: Biotium, Inc.
    Inventors: Fei Mao, Wai-Yee Leung, Xing Xin
  • Patent number: 9464107
    Abstract: Labeled reactant compositions, and particularly labeled nucleic acid reaction compositions that include structural components including double-stranded nucleic acids. In some embodiments, the structural components maintain potentially damaging labeling components sufficiently distal from the reactant portion of the molecule such that damaging effects of the label group on other reaction components, such as enzymes, are reduced, minimized and/or eliminated.
    Type: Grant
    Filed: February 3, 2014
    Date of Patent: October 11, 2016
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Jeffrey Wegener, Jonas Korlach
  • Patent number: 9315537
    Abstract: In summary, the present invention concerns a method for multiple orthogonal labelling of oligonucleotides, preferably RNA or DNA, by simultaneously performing the inverse Diels-Alder reaction (DAinv) and the copper-catalyzed click reaction (CuAAC), wherein the method is employed in a single step by just adding the different reaction components together and incubating the aqueous reaction mixture preferably for one hour at room temperature. In detail, the reaction components are one or more N3-modified labels, a copper compound, a stabilizing ligand, a reducing agent and one or more electron-deficient label-modified dienes that are added together with an at least double-modified oligonucleotide having one more nucleotides containing one or more N3-reactive groups and one or more electron-rich dienophiles, wherein a terminal alkyne moiety is preferably used as N3-reactive group(s) and a frans-cyclooctene moiety or norbornene is preferably used as electron-rich dienophile(s), more preferably frans-cyclooctene.
    Type: Grant
    Filed: August 31, 2012
    Date of Patent: April 19, 2016
    Assignees: DEUTSCHES KREBSFORSCHUNGSZENTRUM, RUPRECHT-KARLS-UNIVERSITAT HEIDELBERG
    Inventors: Juliane Becker, Andres Jäschke, Ayan Samanta, Manfred Wiessler
  • Patent number: 9062091
    Abstract: Compositions and methods are provided for nucleotide analogs comprising protein shields for improving enzyme photostability in single molecule real time sequencing. Nucleotide analogs of the invention have a protein shield between the dye moieties and nucleotide moieties of the analog. The protein prevents the direct interaction of the dye moiety with the enzyme carrying out nucleotide synthesis preventing photodamage to the enzyme. The nucleotide analogs of the invention can have multiple dyes and multiple nucleotide moieties.
    Type: Grant
    Filed: February 14, 2013
    Date of Patent: June 23, 2015
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Keith Bjornson, Jeremiah Hanes, Erik Miller, Satwik Kamtekar, Lubomir Sebo, Louis Brogley
  • Patent number: 9017973
    Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.
    Type: Grant
    Filed: November 28, 2011
    Date of Patent: April 28, 2015
    Assignee: Intelligent BioSystems, Inc.
    Inventors: Steven Gordon, Jerzy Olejnik
  • Patent number: 9018369
    Abstract: The present invention provides a family of dark quenchers, termed Black Hole Quenchers (“BHQs”), that are efficient quenchers of excited state energy but which are themselves substantially non-fluorescent. Also provided are methods of using the BHQs, probes incorporating the BHQs and methods of using the probes.
    Type: Grant
    Filed: January 17, 2014
    Date of Patent: April 28, 2015
    Assignee: Biosearch Technologies, Inc.
    Inventors: Ronald M. Cook, Matt Lyttle, Daren Dick
  • Patent number: 8999639
    Abstract: The present invention relates to methods and compounds for detection of molecular targets, such as biological or chemical molecules, or molecular structures, in samples using a host of experimental schemes for detecting and visualizing such targets, e.g. immunohistochemistry (IHC), in situ hybridization (ISH), ELISA, Southern, Northern, and Western blotting, etc.
    Type: Grant
    Filed: April 4, 2013
    Date of Patent: April 7, 2015
    Assignee: DAKO Denmark A/S
    Inventor: Jesper Lohse
  • Patent number: 8993784
    Abstract: The present disclosure relates to new polymethine compounds and their use as fluorescent labels. The compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.
    Type: Grant
    Filed: July 7, 2014
    Date of Patent: March 31, 2015
    Assignee: Illumina Cambridge Limited
    Inventors: Nikolai Nikolaevich Romanov, Xiaohai Liu
  • Patent number: 8987432
    Abstract: To identify conserved and variable regions of the 16 S rRNA, an instant evolution experiment was performed on the entire 16 S rRNA. Analysis of these mutants identified regions that are required for function. These conserved sequences may be used as targets for pharmaceuticals that are taxonomically specific and which are refractory to the development of drug resistance.
    Type: Grant
    Filed: May 14, 2012
    Date of Patent: March 24, 2015
    Assignee: Wayne State University
    Inventor: Philip R. Cunningham
  • Publication number: 20150072345
    Abstract: Oligonucleotides with a novel sugar-phosphate backbone containing at least one 2?-arabino-fluoronucleoside and an internucleoside 3?-NH—P(—O)(OR)—O-5? linkage, where R is a positively charged counter ion or hydrogen, and methods of synthesizing and using the inventive oligonucleotides are provided. The inventive phosphoramidate 2?-arabino-fluorooligonucleotides have a high RNA binding affinity to complementary nucleic acids and are base and acid stable.
    Type: Application
    Filed: September 11, 2014
    Publication date: March 12, 2015
    Inventors: Sergei M. Gryaznov, Ronald G. Schultz
  • Publication number: 20150072894
    Abstract: The invention provides a novel class of cyanine dyes that are functionalized with sulfonic acid groups and a linker moiety that facilitates their conjugation to other species and substituent groups which increase the water-solubility, and optimize the optical properties of the dyes. Also provided are conjugates of the dyes, methods of using the dyes and their conjugates and kits including the dyes and their conjugates.
    Type: Application
    Filed: September 17, 2014
    Publication date: March 12, 2015
    Applicant: Pacific Biosciences of California, Inc.
    Inventors: Stephen YUE, Gene SHEN, Wei-Chuan (David) SUN
  • Patent number: 8969535
    Abstract: Provided are novel nucleotides, nucleoside, and their derivatives described herein, that can be used in DNA sequencing technology and other types of DNA analysis. In one embodiment, the nucleotide or nucleoside with an unprotected 3?-OH group is derivatized at the nucleobase to include a fluorescent dye attached via a linker to a photocleavable terminating group. The photocleavable-fluorescent group is designed to terminate DNA synthesis as well as be cleaved so that DNA oligomers can be sequenced efficiently in a parallel format. The design of such rapidly cleavable fluorescent groups on nucleotides and nucleosides can enhance the speed and accuracy of sequencing of large oligomers of DNA in parallel, to allow rapid whole genome sequencing, and the identification of polymorphisms and other valuable genetic information, as well as allowing further manipulation and analysis of nucleic acid molecules in their native state following cleavage of the fluorescent group.
    Type: Grant
    Filed: August 23, 2012
    Date of Patent: March 3, 2015
    Assignee: Lasergen, Inc.
    Inventors: Weidong Wu, Vladislav A. Litosh, Brian P. Stupi, Michael L. Metzker
  • Patent number: 8969004
    Abstract: The present invention provides methods for determining the presence of immobilized nucleic acid employing unsymmetrical cyanine dyes that are derivatives of thiazole orange, a staining solution and select fluorogenic compounds that are characterized as being essentially non-genotoxic. The methods comprise immobilizing nucleic acid, single or double stranded DNA, RNA or a combination thereof, on a solid or semi solid support, contacting the immobilized nucleic acid with an unsymmetrical cyanine dye compound and then illuminating the immobilized nucleic acid with an appropriate wavelength whereby the presence of the nucleic acid is determined. The cyanine dye compounds are typically present in an aqueous staining solution comprising the dye compound and a tris acetate or tris borate buffer wherein the solution facilitates the contact of the dye compound and the immobilized nucleic acid.
    Type: Grant
    Filed: May 15, 2013
    Date of Patent: March 3, 2015
    Assignee: Life Technologies Corporation
    Inventors: Matthew Beaudet, William Gregory Cox, Stephen Yue
  • Patent number: 8962820
    Abstract: The present invention relates to combinations of fluorescent dyes used in molecular biology, particularly in multiplex PCR. In particular, the present invention relates to a combination of dyes for amplification reactions, wherein at least four different dyes are used, wherein the first dye is 5-FAM or 6-FAM or a blend thereof, the second dye is selected from the group consisting of DY-530, HEX, CAL Fluor Orange 560 and ATTO 532, the third dye is selected from the group consisting of ATTO 550, DY-555 and DY-556, the fourth dye is selected from the group consisting of ROX, DY-510XL and ATTO 565, and optionally a fifth dye is selected from the group consisting of DY 632 and DY-520XL.
    Type: Grant
    Filed: November 27, 2013
    Date of Patent: February 24, 2015
    Assignee: Qiagen GmbH
    Inventors: Werner Brabetz, Cornelia Weber
  • Patent number: 8957029
    Abstract: The present invention relates to novel peptides capable of binding to action. The peptides are useful in methods for detecting actin in vitro or in living cells.
    Type: Grant
    Filed: November 27, 2008
    Date of Patent: February 17, 2015
    Assignee: Max-Planck-Gesellschaft zur Forderung der Wissenschaften E.V.
    Inventors: Roland Wedlich-Söldner, Michael Sixt, Julia Riedl, Alvaro Crevenna
  • Patent number: 8946404
    Abstract: The present invention provides a family of dark quenchers, termed Black Hole Quenchers (“BHQs”), that are efficient quenchers of excited state energy but which are themselves substantially non-fluorescent. Also provided are methods of using the BHQs, probes incorporating the BHQs and methods of using the probes.
    Type: Grant
    Filed: June 30, 2014
    Date of Patent: February 3, 2015
    Assignee: Biosearch Technologies, Inc.
    Inventors: Ronald M. Cook, Matt Lyttle, Daren Dick
  • Publication number: 20150011712
    Abstract: The present invention relates to diaminophenothiazinium derivatives of formula (I); in which R1, R2, R3, R4, R5, R6 and X? are as defined in Claim 1, and also the methods for labelling oligonucleotides using such a derivative, labeling substrates and the oligonucleotides which can be obtained by means of such methods or from such labelling substrates.
    Type: Application
    Filed: February 21, 2013
    Publication date: January 8, 2015
    Inventors: Carole Chaix, Gabriel De Crozals
  • Patent number: 8927212
    Abstract: FRET-labeled compounds are provided for use in analytical reactions. In certain embodiments, FRET-labeled nucleotide analogs are used in place of naturally occurring nucleoside triphosphates or other analogs in analytical reactions comprising nucleic acids, for example, template-directed nucleic acid synthesis, DNA sequencing, RNA sequencing, single-base identification, hybridization, binding assays, and other analytical reactions.
    Type: Grant
    Filed: March 30, 2010
    Date of Patent: January 6, 2015
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Xiangxu Kong, Gene Shen
  • Patent number: 8916345
    Abstract: A composition comprising an oligonucleotide having the structure 5?-Y1-L1-X-L2-Y2-3?. Y1 comprises a sequence of one or more DNA or RNA nucleotides, including a first nucleotide N1 having a 3? phosphate covalently linked to L1. Y2 comprises a sequence of one or more DNA or RNA nucleotides, including a second nucleotide N2 having a 5? phosphate covalently linked to L2. L1 and L2 each independently are a direct bond or a C1-C7 alkyl, alkynyl, alkenyl, heteroalkyl, substituted alkyl, aryl, heteroaryl, substituted aryl, cycloalkyl, alkylaryl, or alkoxyl group. X is R1 is a hydrogen or a C1-C8 alkyl. M is a label or ligand comprising a fused polycyclic aromatic moiety.
    Type: Grant
    Filed: March 28, 2011
    Date of Patent: December 23, 2014
    Assignee: Integrated DNA Technologies, Inc.
    Inventors: Scott Rose, Mark A. Behlke, Richard Owczarzy, Joseph A. Walder, Derek M. Thomas, Michael R. Marvin
  • Patent number: 8906614
    Abstract: Labeled reactant compositions, and particularly labeled nucleic acid reaction compositions, that include structural components that maintain potentially damaging labeling components sufficiently distal from the reactant portion of the molecule such that damaging effects of the label group on other reaction components, such as enzymes, are reduced, minimized and/or eliminated.
    Type: Grant
    Filed: November 29, 2012
    Date of Patent: December 9, 2014
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Jeffrey Wegener, Jonas Korlach
  • Patent number: 8877437
    Abstract: Methods of using dyes and associated technology are provided. A dye, such as a monomeric dye or a dimeric dye, may be used in a nucleic acid gel staining application and/or a nucleic acid detection application. A dimeric dye, such as a dimeric dye capable of forming a hairpin-like structure, may be used to stain and/or detect nucleic acids via a release-on-demand mechanism. A dimeric dye having low background fluorescence in the absence of nucleic acids and high fluorescence in the presence of nucleic acids, upon binding therewith, may be used to stain and/or detect nucleic acids. Buffers comprising a weak acid and a salt of the weak acid, such as a lithium salt, are also provided to allow for effective prestaining of nucleic acids.
    Type: Grant
    Filed: December 22, 2010
    Date of Patent: November 4, 2014
    Assignee: Biotium, Inc.
    Inventors: Fei Mao, Wai-Yee Leung, Tam Van
  • Publication number: 20140322713
    Abstract: The present disclosure relates to new polymethine compounds and their use as fluorescent labels. The compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.
    Type: Application
    Filed: July 7, 2014
    Publication date: October 30, 2014
    Applicant: Illumina Cambridge Limited
    Inventors: Nikolai Nikolaevich Romanov, Xiaohai Liu
  • Patent number: 8871921
    Abstract: Embodiments of the present disclosure relate generally to reporter compositions which are synthetic nucleotides that comprise nucleotides with a high charge mass moiety attached thereto via a linker molecule. The linker molecules can vary in length in part to enable the high charge mass moiety to extend out from a DNA polymerase complex so that polymerization may not be influenced.
    Type: Grant
    Filed: September 3, 2009
    Date of Patent: October 28, 2014
    Assignee: Quantumdx Group Ltd.
    Inventor: Jonathan O'Halloran
  • Patent number: 8846881
    Abstract: Nucleic acid compositions, methods of making and using such compositions that comprise modular functional groups that can be configured to provide desired functionality to different nucleotide types, through a swappable and preferably non-covalent linkage component. Such compositions are useful in a variety of applications including nucleic acid analyses.
    Type: Grant
    Filed: July 19, 2012
    Date of Patent: September 30, 2014
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Jonas Korlach, Jeffrey Wegener
  • Patent number: 8846883
    Abstract: Oligonucleotide chemistry is central to the advancement of core technologies such as DNA sequencing, forensic and genetic analysis and has impacted greatly on the discipline of molecular biology. Oligonucleotides and their analogues are essential tools in these areas. They are often produced by automated solid-phase phosphoramidite synthesis but it is difficult to synthesize long DNA and RNA sequences by this method. Methods are proposed for ligating oligonucleotides together, in particular the use of an azide-alkyne coupling reaction to ligate the backbones of oligonucleotides together to form longer oligonucleotides that can be synthesized using current phosphoramidite synthesis methods.
    Type: Grant
    Filed: March 1, 2012
    Date of Patent: September 30, 2014
    Assignee: University of Southhampton
    Inventors: Tom Brown, Afaf Helmy El-Sagheer
  • Patent number: 8841072
    Abstract: Methods, compositions and articles of manufacture for assaying a sample for a target polynucleotide are provided. A sample suspected of containing the target polynucleotide is contacted with a polycationic multichromophore and a sensor polynucleotide complementary to the target polynucleotide. The sensor polynucleotide comprises a signaling chromophore to receive energy from the excited multichromophore and increase emission in the presence of the target polynucleotide. The methods can be used in multiplex form. Kits comprising reagents for performing such methods are also provided.
    Type: Grant
    Filed: November 21, 2013
    Date of Patent: September 23, 2014
    Assignee: The Regents of the University of California
    Inventors: Guillermo C. Bazan, Brent S. Gaylord, Shu Wang
  • Patent number: 8822673
    Abstract: Methods and compositions are provided for detecting a primer extension product in a reaction mixture. In the subject methods, a primer extension reaction is conducted in the presence of a polymerase having 3??5? exonuclease activity and at least one FET labeled oligonucleotide probe that includes a 3??5? exonuclease resistant quencher domain. Also provided are systems and kits for practicing the subject methods. The subject invention finds use in a variety of different applications, and are particularly suited for use in high fidelity PCR based reactions, including SNP detection applications, allelic variation detection applications, and the like.
    Type: Grant
    Filed: November 12, 2010
    Date of Patent: September 2, 2014
    Assignee: Life Technologies Corporation
    Inventors: Quin Chou, Dragan Spasic
  • Patent number: 8815509
    Abstract: The inventors have developed a rapid and sensitive fluorescence-based assay to quantify dNTPs. This assay relies on the principle that incorporation of a limiting dNTP is required for primer-extension and polymerase-mediated 5-3? exonuclease hydrolysis of a quenched fluorophore-labeled probe resulting in fluorescence. The concentration of limiting dNTPs is directly proportional to the fluorescence generated. This assay has important applications in research that investigates the influence of pathological conditions or pharmacological agents on dNTP biosynthesis and regulation.
    Type: Grant
    Filed: June 14, 2012
    Date of Patent: August 26, 2014
    Assignee: University of Southern California
    Inventors: Peter M. Wilson, Robert D. Ladner
  • Patent number: 8809551
    Abstract: The present disclosure relates to new polymethine compounds and their use as fluorescent labels. The compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.
    Type: Grant
    Filed: March 8, 2013
    Date of Patent: August 19, 2014
    Assignee: Illumina Cambridge Limited
    Inventors: Nikolai Nikolaevich Romanov, Xiaohai Liu
  • Patent number: 8796432
    Abstract: This invention provides a nucleotide analogue comprising (i) a base selected from the group consisting of adenine, guanine, cytosine, thymine and uracil, (ii) a deoxyribose, (iii) an allyl moiety bound to the 3?-oxygen of the deoxyribose and (iv) a fluorophore bound to the base via an allyl linker, and methods of nucleic acid sequencing employing the nucleotide analogue.
    Type: Grant
    Filed: October 31, 2006
    Date of Patent: August 5, 2014
    Assignee: The Trustees of Columbia University in the City of New York
    Inventors: Jingyue Ju, Lanrong Bi, Dae H. Kim, Qinglin Meng
  • Publication number: 20140187434
    Abstract: Nucleic acid labeling compounds are disclosed. The compounds are synthesized by condensing a heterocyclic derivative with a cyclic group (e.g. a ribofuranose derivative). The labeling compounds are suitable for enzymatic attachment to a nucleic acid, either terminally or internally, to provide a mechanism of nucleic acid detection.
    Type: Application
    Filed: June 27, 2013
    Publication date: July 3, 2014
    Applicant: Affymetrix, Inc.
    Inventors: Glenn Hugh McGall, Anthony Dale Barone
  • Patent number: 8765369
    Abstract: The invention relates to methods and reagents for detecting minute amounts of targets having affinity for nucleic acid. The present invention more particularly relates to target detection using aggregates of cationic polymer chains and nucleic acid capture probes linked to particles, such as controllable mobility particles.
    Type: Grant
    Filed: July 11, 2008
    Date of Patent: July 1, 2014
    Assignee: National Research Council of Canada
    Inventors: Sebastien Dubus, Hoang-Anh Ho, Mario Leclerc, Denis Boudreau
  • Publication number: 20140178878
    Abstract: The present application discloses a labeled nucleotide comprising a label attached via a linker, wherein said labeled nucleotide has the formula wherein R1 is a residue with a negative net charge, preferably selected from the group consisting of a phosphate group, and a sulphate group; wherein R2, R3 and R4 are independently selected from the group consisting of H2, OH2, and O; wherein “n” is an integer between 0 and 16; wherein “a” is an integer between 1 and 10; wherein SP is absent or a spacer; wherein X is said label; and wherein Y is a nucleotide or nucleoside. Furthermore, oligonucleotides comprising a labeled nucleotide according to the present invention and the use as a primer in amplification based methods is disclosed herein.
    Type: Application
    Filed: May 4, 2012
    Publication date: June 26, 2014
    Inventors: Daniel Müller, Francesca Di Pasquale
  • Publication number: 20140171635
    Abstract: Novel Hydrazone-based Fluorescent and Pro-Fluorescent Reagents and Linkers, including conjugationally extended hydrazine compositions, fluorescent hydrazone compositions, methods of the formation of hydrazones from the reaction of conjugationally extended hydrazines with conjugationally extended carbonyls, and methods of their use in assays systems are described. Use of these conjugationally compositions for direct colorimetric and fluorometric assays wherein a chromophore or the fluorophore is incorporated into the linker that is positioned between a reactive linking moiety and a biotin molecule.
    Type: Application
    Filed: August 14, 2013
    Publication date: June 19, 2014
    Applicant: Solulink Biosciences, Inc.
    Inventors: David A. Schwartz, Leopold Mendoza
  • Patent number: 8742084
    Abstract: This invention relates to agents and conjugates that can be used to detect and isolate target components from complex mixtures such as nucleic acids from biological samples, cells from bodily fluids, and nascent proteins from translation reactions. The invention also relates to targets isolated with conjugates which may be useful as pharmaceutical agents or compositions that can be administered to humans and other mammals. The invention further relates to kits comprised of agents and conjugates that can be used for the detection of diseases, disorders and individual substances in a complex background of substances.
    Type: Grant
    Filed: September 14, 2012
    Date of Patent: June 3, 2014
    Assignee: The Trustees of Boston University
    Inventors: Kenneth J. Rothschild, Sanjay M. Sonar, Jerzy Olejnik
  • Patent number: 8716457
    Abstract: The present invention provides modified nucleobase compounds, modified nucleic acid mimetic compounds and various uses thereof. In addition, the invention provides methods for nucleobase characterisation, SNP characterisation and nucleic acid sequencing.
    Type: Grant
    Filed: September 19, 2008
    Date of Patent: May 6, 2014
    Assignee: The University Court of the University of Edinburgh
    Inventors: Mark Bradley, Juan J. Diaz-Mochon
  • Patent number: 8709721
    Abstract: Provided are a fluorescent on/off switchable compound for a gene analysis, which is highly stable and highly sensitive, and enables amplification and observation of a trace gene signal, and a labeling reagent for detection of a bio-related material, which uses the fluorescent on/off switchable compound. A compound represented by the following formula (I?): wherein X is a hydrogen atom or a carboxylic acid-protecting group, Y is a 2,4-dinitrobenzenesulfonyl group, a 2-cyano-4-nitrobenzenesulfonyl group or a 5-nitropyridin-2-ylsulfonyl group, R1, R2, R3 and R4 are each independently a hydrogen atom, a halogen atom, a hydroxyl group, an alkyl group having a carbon number of 1 to 6, an alkoxy group having a carbon number of 1 to 6, an aryl group having a carbon number of 6 to 10, or a cyano group.
    Type: Grant
    Filed: October 1, 2010
    Date of Patent: April 29, 2014
    Assignee: Riken
    Inventors: Hiroshi Abe, Yoshihiro Ito, Aya Shibata, Mika Ito
  • Patent number: 8709722
    Abstract: There is provided a method for detecting binding of a DNA-binding protein to a target recognition sequence. The method comprises mixing in a reaction buffer a first set of metal nanoparticles, a second set of metal nanoparticles and a DNA-binding protein to form a mixture, and detecting the aggregation state of the mixture of metal nanoparticles. Each set of metal nanoparticles has a conjugated double-stranded DNA molecule having a single-stranded overhang at one end. The single-stranded overhangs of each set of DNA-conjugated metal nanoparticles are complementary to each other such that annealing of the complementary overhangs results in formation of the target recognition sequence that specifically binds the DNA-binding protein. The reaction buffer comprises an ionic species in a concentration sufficient to result in aggregation of the metal nanoparticles upon annealing of the first and second single-stranded overhang.
    Type: Grant
    Filed: August 24, 2012
    Date of Patent: April 29, 2014
    Assignee: Agency for Science, Technology and Research
    Inventors: Yen Nee Tan, Xiaodi Su
  • Publication number: 20140080127
    Abstract: The present invention provides heteroaryl functionalized cyanine dyes including a reactive functional moiety, or which are conjugated to a carrier molecule.
    Type: Application
    Filed: November 20, 2013
    Publication date: March 20, 2014
    Applicant: Pacific Biosciences of California, Inc.
    Inventors: Stephen YUE, Gene Shen, Wei-Chuan Sun
  • Publication number: 20140080122
    Abstract: Systems and methods related to optical nanosensors comprising photoluminescent nanostructures are generally described. Generally, the nanosensors comprise a photoluminescent nanostructure and a polymer that interacts with the photoluminescent nanostructure. In some cases, the interaction between the polymer and the nanostructure can be non-covalent (e.g., via van der Waals interactions). The nanosensors comprising a polymer and a photoluminescent nanostructure may be particularly useful in determining the presence and/or concentration of relatively small molecules, in some embodiments. In addition, in some instances the nanosensors may be capable of determining relatively low concentrations of analytes, in some cases determining as little as a single molecule. In some embodiments, the interaction between the analyte and the nanosensor (e.g.
    Type: Application
    Filed: July 15, 2013
    Publication date: March 20, 2014
    Applicant: Massachusetts Institute of Technology
    Inventors: Michael S. Strano, Jong-Ho Kim, Jinqing Zhang, Daniel A. Heller
  • Publication number: 20140072515
    Abstract: Compounds used as labels with properties comparable to known fluorescent compounds. The compounds can be conjugated to proteins and nucleic acids for biological imaging and analysis. Synthesis of the compounds, formation and use of the conjugated compounds, and specific non-limiting examples of each are provided.
    Type: Application
    Filed: February 28, 2013
    Publication date: March 13, 2014
    Inventors: Greg Hermanson, Peter T. Czerney, Surbhi Desai, Matthias S. Wenzel, Boguslawa R. Dworecki, Frank G. Lehmann, Marie Christine Nlend
  • Patent number: 8660753
    Abstract: The present invention relates to a control method of a wheel alignment apparatus using an MDPS, which determines whether or not to cancel center alignment control due to a trouble or error is preferentially determined prior to each control step and then performs control when wheels of a vehicle having an MDPS mounted therein are aligned, such that the trouble or error is preferentially considered in the control priority, thereby increasing driver's convenience and improving safety performance for protecting the driver.
    Type: Grant
    Filed: May 2, 2013
    Date of Patent: February 25, 2014
    Assignee: Hyundai Mobis Co., Ltd.
    Inventor: Jeong Ku Kim
  • Publication number: 20140039178
    Abstract: The present invention is directed towards the synthesis of high purity deuterated sugars, deuterated phosphoramidites, deuterated nucleobases, deuterated nucleosides, deuterated oligonucleotides, and deuterated RNA's of defined sequences which can exhibit biochemically useful and biologically valuable properties, thus having potential for therapeutic uses.
    Type: Application
    Filed: July 31, 2012
    Publication date: February 6, 2014
    Inventors: Suresh C. Srivastava, Amy Yasko
  • Patent number: 8642561
    Abstract: A generic structure for the peptides of the present invention includes A-X-B-C, where C is a cargo moiety, the B portion includes basic amino acids, X is a cleavable linker sequence, and the A portion includes acidic amino acids. The intact structure is not significantly taken up by cells; however, upon extracellular cleavage of X, the B-C portion is taken up, delivering the cargo to targeted cells. Cargo may be, for example, a contrast agent for diagnostic imaging, a chemotherapeutic drug, or a radiation-sensitizer for therapy. Cleavage of X allows separation of A from B, unmasking the normal ability of the basic amino acids in B to drag cargo C into cells near the cleavage event. X is cleaved extracellularly, preferably under physiological conditions. D-amino acids are preferred for the A and B portions, to minimize immunogenicity and nonspecific cleavage by background peptidases or proteases.
    Type: Grant
    Filed: December 7, 2011
    Date of Patent: February 4, 2014
    Assignee: The Regents of the University of California
    Inventors: Tao Jiang, Roger Y. Tsien