Labelled (e.g., Tagged With Radioactive Tracer, Fluorescent Marker, Intercalator, Etc.) Patents (Class 536/26.6)
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Patent number: 7863058Abstract: Disclosed are methods of attaching biologically active compounds to a solid surface, comprising modifying the solid surface using triazine chloride and attaching the biologically active compound to the triazine moiety.Type: GrantFiled: February 2, 2009Date of Patent: January 4, 2011Assignee: Illumina, Inc.Inventors: Chanfeng Zhao, Igor Kozlov, Galina Steinberg-Tatman
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Patent number: 7858320Abstract: A method for labeling biological molecules and synthetic molecules that contain a geminal diol, such as RNA, carbohydrates or glycoproteins, using a periodate sequestering agent is provided. Compositions and kits for use in the labeling method are also provided.Type: GrantFiled: October 31, 2008Date of Patent: December 28, 2010Assignee: BioVentures, Inc.Inventors: Elliott P. Dawson, Kristie E. Womble
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Patent number: 7851159Abstract: It is intended to provide a method for detecting a target nucleic acid with a specific base sequence existing in a sample mixture with high specificity and sensitivity by utilizing the formation of a hybrid with a complementary strand as a detection principle, and a nucleic acid for the detection.Type: GrantFiled: November 17, 2006Date of Patent: December 14, 2010Assignee: Japan Science and Technology AgencyInventors: Kenzo Fujimoto, Yoshinaga Yoshimura, Shinzi Ogasawara
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Patent number: 7851155Abstract: This invention pertains to the field of combination oligomers, including the block synthesis of combination oligomers in the absence of a template, as well as related methods, kits, libraries and other compositions.Type: GrantFiled: December 26, 2006Date of Patent: December 14, 2010Assignee: Boston Probes, Inc.Inventors: James M. Coull, Mark J. Fiandaca, Mark D. Kristjanson, Jens J. Hyldig-Nielsen, Theresa S. Creasey
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Publication number: 20100311178Abstract: The invention provides a nucleotide or nucleoside having a base attached to a detectable label via a cleavable linker, characterised in that the cleavable linker contains a moiety selected from the group comprising: Formula (I) (wherein X is selected from the group comprising O, S, NH and NQ wherein Q is a C1-10 substituted or unsubstituted alkyl group, Y is selected from the group comprising O, S, NH and N(allyl). T is hydrogen or a C1-10 substituted or unsubstituted alkyl group and * indicates where the moiety is connected to the remainder of the nucleotide or nucleoside).Type: ApplicationFiled: June 21, 2010Publication date: December 9, 2010Inventors: Xiaohai Liu, John Milton, Silke Ruediger
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Patent number: 7847075Abstract: Within oligonucleotides, 2-azapurine and especially 2-azaadenine bases form specifically base pairs with guanine. This base pair is of analogous stability as an adenine-thymine but less stable than a guanine-cytosine base pair. Therefore, the incorporation of 2-azaadenine residues into oligonucleotides instead of cytosine leads specifically to hybridization complexes with nucleic acids with homogenous stability. This is useful for the adaptation of the stabilities of different oligonucleotide sequences in all kinds of hybridization techniques, for example in oligomer chip technology.Type: GrantFiled: August 13, 2009Date of Patent: December 7, 2010Assignee: Roche Molecular Systems, Inc.Inventors: Frank Seela, Helmut Rosemeyer, Enno Schweinberger, Dieter Heindl, Frank Bergmann
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Patent number: 7847076Abstract: An oligonucleotide probe is disclosed, the probe including an oligonucleotide, a fluorescer molecule attached to a first end of the oligonucleotide and a quencher molecule attached to the opposite end of the oligonucleotide. The probe is rendered impervious to digestion by the 5??3? exonuclease activity of a polymerase and the 5??3? extension of by a polymerase. The invention also includes methods for performing combined PCR amplification and hybridization probing, one such method including the steps of contacting a target nucleic acid sequence with PCR reagents and an oligonucleotide probe as described above, and subjecting these reagents to thermal cycling. One preferred refinement of the above method further includes the addition of a strand displacer to facilitate amplification.Type: GrantFiled: August 18, 2008Date of Patent: December 7, 2010Assignee: Life Technologies CorporationInventor: Paul E. Mayrand
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Patent number: 7842811Abstract: Cyanine dye compounds having a negatively charged substituent that are nucleic acid stains, particularly for fluorescent staining of DNA, including compounds having the formula wherein W forms one or two fused 5- or 6-membered aromatic rings, ? has a value of 0 or 1, n has a value of 0, or 1, X is O, S, or Se, and D is a pyridinium, or quinolinium moiety, provided that the compound has at least one negatively charged substituent.Type: GrantFiled: January 12, 2010Date of Patent: November 30, 2010Assignee: Life Technologies CorporationInventors: Jason Dallwig, David Hagen, Shih-Jung Huang, Gerald Thomas, Stephen Yue
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Publication number: 20100292457Abstract: The invention provides novel dye-labeled ribonucleotide analogs and methods for synthesizing those analogs. The compounds of the invention are especially useful for DNA sequencing by the polymerase chain reaction.Type: ApplicationFiled: January 25, 2010Publication date: November 18, 2010Applicant: Applied Biosystems, LLCInventors: Peter Virgil Fisher, Paolo Vatta, Shaheer H. Khan
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Publication number: 20100290990Abstract: The present invention relates to a method for preparing a 2-fluoropurine marked with the radioisotope 18F comprising a fluorination step for a 2-nitropurine derivative. The present invention comprises a 2-fluoropurine derivative marked with the radioisotope 18F which can be obtained by or during a method according to the invention and its various uses.Type: ApplicationFiled: December 23, 2008Publication date: November 18, 2010Inventors: Louisa Barre, Patrice Marchand
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Patent number: 7833756Abstract: Dibenzorhodamine compounds having the structure are disclosed, including nitrogen- and aryl-substituted forms thereof. In addition, two intermediates useful for synthesizing such compounds are disclosed, a first intermediate having the structure including nitrogen- and aryl-substituted forms thereof, and a second intermediate having the structure including nitrogen- and aryl-substituted forms thereof, wherein substituents at positions C-14 to C18 taken separately are selected from the group consisting of hydrogen, chlorine, fluorine, lower alkyl, carboxylic acid, sulfonic acid, —CH2OH, alkoxy, phenoxy, linking group, and substituted forms thereof. The invention further includes energy transfer dyes comprising the dibenzorhodamine compounds, nucleosides labeled with the dibenzorhodamine compounds, and nucleic acid analysis methods employing the dibenzorhodamine compounds.Type: GrantFiled: July 8, 2008Date of Patent: November 16, 2010Assignee: Applied Biosystems, LLCInventors: Scott C. Benson, Joe Y. L. Lam, Steven Michael Menchen
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Publication number: 20100286386Abstract: Nucleotide analogues comprising a reactive hydrazide function (formula II) used as initial synthons for preparing compounds (formula I) capable of inducing mutations or capable of inhibiting a DNA polymerase or a kinase provided. Nucleic acids comprising the nucleotide and nucleoside analogues are also provided. Methods of using the compounds are also provided.Type: ApplicationFiled: November 14, 2007Publication date: November 11, 2010Inventors: Philippe Marliere, Sylvie Pochet
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Patent number: 7824863Abstract: In one aspect of the invention, a method is provided for end-labeling RNA (total RNA, mRNA, cRNA or fragmented RNA). In one aspect of the present invention, T4 RNA ligase is used to attach a 3?-labeled AMP or CMP donor to an RNA acceptor molecule. In another embodiment, a pyrophosphate molecule 3?-AppN-3?-linker-detectable moiety is used as donor molecule.Type: GrantFiled: October 22, 2008Date of Patent: November 2, 2010Assignee: Affymetrix, Inc.Inventors: Kyle B. Cole, Vivi Truong, Glenn H. McGall, Anthony D. Barone
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Patent number: 7824887Abstract: Disclosed herein a reversible terminator nucleotides and methods of use.Type: GrantFiled: June 26, 2009Date of Patent: November 2, 2010Assignee: Applied Biosystems LLCInventors: Linda Lee, Gerald Zon
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Patent number: 7825237Abstract: Novel linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye are provided. These linkers facilitate the efficient transfer of energy between a donor and acceptor dye in an energy transfer dye. One of these linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye has the general structure R21Z1C(O)R22R28 where R21 is a C1-5 alkyl attached to the donor dye, C(O) is a carbonyl group, Z1 is either NH, sulfur or oxygen, R22 is a substituent which includes an alkene, diene, alkyne, a five and six membered ring having at least one unsaturated bond or a fused ring structure which is attached to the carbonyl carbon, and R28 includes a functional group which attaches the linker to the acceptor dye.Type: GrantFiled: September 4, 2009Date of Patent: November 2, 2010Assignee: Applied Biosystems, LLCInventors: Linda Lee, Sandra Spurgeon, Barnett Rosenblum
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Patent number: 7820391Abstract: A method of detecting a target nucleic acid.Type: GrantFiled: November 6, 2008Date of Patent: October 26, 2010Assignee: Osmetech Molecular DiagnosticsInventor: Tao Chunlin
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Patent number: 7816503Abstract: The invention is directed to modified guanine-containing nucleosides and nucleotides and uses thereof. More specifically, the invention relates to modified fluorescently labelled guanine-containing nucleosides and nucleotides which exhibit enhanced fluorophore intensity by virtue of reduced quenching effects.Type: GrantFiled: September 21, 2009Date of Patent: October 19, 2010Assignee: Illumina Cambridge LimitedInventors: John Milton, Xiaohai Liu
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Patent number: 7816501Abstract: This invention is directed to compositions comprising a linked acceptor moiety.Type: GrantFiled: June 30, 2003Date of Patent: October 19, 2010Assignee: Boston Probes, Inc.Inventors: James M. Coull, Brian D. Gildea, Jens J. Hyldig-Nielsen
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Patent number: 7811755Abstract: The invention relates to methods, articles and compositions for the detection and analysis of biomolecules in a sample. Provided assays include those determining the presence of a target biomolecule in a sample or its relative amount, or the assays may be quantitative or semi-quantitative. The methods can be performed in an array format on a substrate.Type: GrantFiled: January 10, 2006Date of Patent: October 12, 2010Assignee: The Regents of the University of CaliforniaInventors: Guillermo C. Bazan, Bin Liu
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Patent number: 7803934Abstract: The present invention provides massively parallel oligonucleotide synthesis and purification for applications that utilize large collections of defined high-fidelity oligonucleotides (e.g., from about 101 to about 105 different sequences, generally between 25-160 bases in length).Type: GrantFiled: January 28, 2009Date of Patent: September 28, 2010Assignee: Affymetrix, Inc.Inventors: Glenn H. McGall, Robert G. Kuimelis
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Patent number: 7803943Abstract: Methods of using dyes and associated technology are provided. A dye, such as a monomeric dye or a dimeric dye, may be used in a nucleic acid gel staining application and/or a nucleic acid detection application. Such a dye and a salt that comprises an anion that is associated with a strong acid and a cation that is associated with a strong base may be used in such an application. A dimeric dye, such as a dimeric dye capable of forming a hairpin-like structure, may be used to stain and/or detect nucleic acids via a release-on-demand mechanism. A dimeric dye having low background fluorescence in the absence of nucleic acids and high fluorescence in the presence of nucleic acids, upon binding therewith, may be used to stain and/or detect nucleic acids.Type: GrantFiled: June 15, 2009Date of Patent: September 28, 2010Assignee: Biotium, Inc.Inventors: Fei Mao, Wai-Yee Leung
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Patent number: 7803936Abstract: The invention provides a novel method of labeling oligonucleotides, with reporter moieties, including but not limited to, quenchers, fluorophores, biotin, digoxigenin, peptides and proteins. In addition, this invention provides a method of detecting hybridization of oligonucleotides. This invention also provides novel azo quenchers having the general formula shown below. The invention further provides compositions comprising labeled oligonucleotides and solid supports. The invention also provides kits comprising at least one composition of the present invention.Type: GrantFiled: November 23, 2009Date of Patent: September 28, 2010Assignee: Integrated DNA Technologies, Inc.Inventors: Andrei Laikhter, Joseph A. Walder, Mark Behlke, Mikhail Podyminogin, Yawfui Yong
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Patent number: 7799912Abstract: A bifunctional compound comprising a molecular unit (I) intercalating between nucleobases (B) of nucleic acids, an active molecular unit (AD) capable of emitting a detectable signal, and optionally a spacer unit, in which the active molecular unit (AD) is selected from amongst chemical entities having a structure such as to interact electronically with the intercalating molecular unit (I) in such a way that, during the reaction of oxidation, the reduction-oxidation potential (EI+/I) of the semicouple I+/I defined by the intercalating molecular unit (I) is lower than the reduction-oxidation potential (EB+/B) of the semicouple B+/B defined by the nucleobases (B), and in such a way that, during the reaction of reduction, the reduction-oxidation potential (EI/I?) of the semicouple I/I? defined by the intercalating molecular unit (I) is higher than the reduction-oxidation potential (EB/B?) of the semicouple B/B? defined by the nucleobases (B).Type: GrantFiled: October 19, 2007Date of Patent: September 21, 2010Assignee: STMicroelectronics S.r.l.Inventors: Sabrina Conoci, Salvatore Sortino
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Patent number: 7795042Abstract: The present invention provides novel, water-soluble, red-emitting fluorescent rhodamine dyes and red-emitting fluorescent energy-transfer dye pairs, as well as labeled conjugates comprising the same and methods for their use. The dyes, energy-transfer dye pairs and labeled conjugates are useful in a variety of aqueous-based applications, particularly in assays involving staining of cells, protein binding, and/or analysis of nucleic acids, such as hybridization assays and nucleic acid sequencing.Type: GrantFiled: March 12, 2009Date of Patent: September 14, 2010Assignee: Applied Biosystems, LLCInventors: Linda G. Lee, Ronald J. Graham, William E. Werner, Elana Swartzman, Lily Lu
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Patent number: 7795424Abstract: The invention provides a nucleotide or nucleoside having a base attached to a detectable label via a cleavable linker, characterized in that the cleavable linker contains a moiety selected from the group comprising: Formula (I) (wherein X is selected from the group comprising O, S, NH and NQ wherein Q is a C1-10 substituted or unsubstituted alkyl group, Y is selected from the group comprising O, S, NH and N(allyl). T is hydrogen or a C1-10 substituted or unsubstituted alkyl group and * indicates where the moiety is connected to the remainder of the nucleotide or nucleoside).Type: GrantFiled: July 24, 2008Date of Patent: September 14, 2010Assignee: Illumina Cambridge LimitedInventors: Xiaohai Liu, John Milton, Silke Ruediger
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Patent number: 7790869Abstract: This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3?-position of the deoxyribose.Type: GrantFiled: June 5, 2007Date of Patent: September 7, 2010Assignee: The Trustees of Columbia University in the City of New YorkInventors: Jingyue Ju, Zengmin Li, John Robert Edwards, Yasuhiro Itagaki
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Patent number: 7785796Abstract: Nucleosides and nucleotides are disclosed that are linked to detectable labels via a cleavable linker group.Type: GrantFiled: July 23, 2009Date of Patent: August 31, 2010Assignee: Illumina Cambridge LimitedInventors: Shankar Balasubramanian, Colin Barnes, Xiaohai Liu, John Milton
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Patent number: 7777013Abstract: Labeled nucleotide analogs used in place of naturally occurring nucleoside triphosphates or other analogs in template directed nucleic acid synthesis reactions and other nucleic acid reactions, and various analyses based thereon, including DNA sequencing, single base identification, hybridization assays and others.Type: GrantFiled: May 15, 2008Date of Patent: August 17, 2010Assignee: Pacific Biosciences of California, Inc.Inventors: Yue Xu, Jeffrey Wegener, Arek Bibillo
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Patent number: 7776567Abstract: Dimeric and trimeric nucleic acid dyes, and associated systems and methods are provided. Such a dye may form a hairpin-like structure that enables it to stain nucleic acids via a release-on-demand mechanism, for example. Such a dye may have low background fluorescence in the absence of nucleic acids and high fluorescence in the presence of nucleic acids, upon binding therewith, for example. A dye provided herein may be useful in a variety of applications, such as in DNA quantitation in real-time PCR, for example.Type: GrantFiled: March 16, 2006Date of Patent: August 17, 2010Assignees: Biotium, Inc., AlleLogic Biosciences CorporationInventors: Fei Mao, Wai-Yee Leung, Xing Xin
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Patent number: 7772384Abstract: Nucleosides and nucleotides are disclosed that are linked to detectable labels via a cleavable linker group.Type: GrantFiled: September 9, 2008Date of Patent: August 10, 2010Assignee: Illumina Cambridge LimitedInventors: Shankar Balasubramanian, Colin Barnes, Xiaohai Liu
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Patent number: 7771973Abstract: The invention provides modified nucleotide or nucleoside molecule comprising a purine or pyrimidine base and a ribose or deoxyribose sugar moiety having a removable 3?-OH blocking group covalently attached thereto, such that the 3? carbon atom has attached a group of the structure —O—Z wherein Z is any of —C(R?)2-O—R?, —C(R?)2-N(R?)2, —C(R?)2-N(H)R?, —C(R?)2-S—R? and —C(R?)2-F, wherein each R? is or is part of a removable protecting group; each R? is independently a hydrogen atom, an alkyl, substituted alkyl, arylalkyl, alkenyl, alkynyl, aryl, heteroaryl, heterocyclic, acyl, cyano, alkoxy, aryloxy, heteroaryloxy or amido group, or a detectable label attached through a linking group; or (R?)2 represents an alkylidene group of formula ?C(R??)2 wherein each R?? may be the same or different and is selected from the group comprising hydrogen and halogen atoms and alkyl groups; and wherein said molecule may be reacted to yield an intermediate in which each R? is exchanged for H or, where Z is —C(R?)2-F, the F is exType: GrantFiled: June 1, 2009Date of Patent: August 10, 2010Assignee: Illumina Cambridge LimitedInventors: John Milton, Xiaolin Wu, Mark Smith, Joseph Brennan, Colin Barnes, Xiaohai Liu, Silke Ruediger
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Patent number: 7767805Abstract: The invention provides nucleotide analogs and methods of using them in sequencing reactions.Type: GrantFiled: May 2, 2008Date of Patent: August 3, 2010Assignee: Helicos Biosciences CorporationInventor: Philip Richard Buzby
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Patent number: 7759061Abstract: This disclosure provides novel reversibly terminated ribonucleotides which can be used as a reagent for DNA sequencing reactions. Methods of sequencing nucleic acids using the disclosed nucleotides are also provided.Type: GrantFiled: June 2, 2005Date of Patent: July 20, 2010Assignee: TwistDx, Inc.Inventors: Olaf Piepenburg, Derek L. Stemple, Niall A. Armes
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Patent number: 7759470Abstract: The current invention restates substituted indole nucleosides as both terminal as well as internal building blocks of labeled oligonucleotide probes for the detection, analysis and quantitation of nucleic acids. The substituent comprises a linker and a detectable group or a linker and a reactive group for post synthesis coupling. These modified nucleosides grant access to a wide application area. These new substituted indole nucleosides can be used as labeling reagents for the facile preparation of e.g. optimized hybridization probes, Taqman-probes, or molecular beacon probes.Type: GrantFiled: February 16, 2007Date of Patent: July 20, 2010Assignee: Roche Diagnostics Operations, Inc.Inventors: Dieter Heindl, Frank Bergmann, Hans-Peter Josel, Dirk Kessler, Gunter Lampert, Christian Weilke
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Patent number: 7759469Abstract: The current invention restates substituted nitroindole nucleosides as both terminal as well as internal building blocks of labeled oligonucleotide probes for the detection, analysis and quantitation of nucleic acids. The substituent comprises a linker and a detectable group or a linker and a reactive group for post synthesis coupling. These modified nucleosides grant access to a wide application area. These new substituted nitroindole nucleosides can be used as labeling reagents for the facile preparation of, e.g., optimized hybridization probes, simple probes, TAQMAN-probes or molecular beacon probes.Type: GrantFiled: March 8, 2006Date of Patent: July 20, 2010Assignee: Roche Diagnostics Operations, Inc.Inventors: Dieter Heindl, Thomas Froehlich, Heribert Maerz, Monika Seller
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Publication number: 20100174058Abstract: Oligonucleotide probes containing two labels are provided and are useful in hybridization assays. The probes can also contain a minor groove binding group.Type: ApplicationFiled: January 6, 2010Publication date: July 8, 2010Inventors: Michael W. Reed, Eugeny Alexander Lukhtanov, Alexander A. Gall, Robert O. Dempcy, Nicolaas M.J. Vermeulen
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Patent number: 7745126Abstract: This invention pertains to the field of combination oligomers, including the block synthesis of combination oligomers in the absence of a template, as well as related methods, kits, libraries and other compositions.Type: GrantFiled: December 26, 2006Date of Patent: June 29, 2010Assignee: Boston Probes, Inc.Inventors: James M. Coull, Mark J. Fiandaca, Mark D. Kristjanson, Jens J. Hyldig-Nielsen, Theresa S. Creasey
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Patent number: 7745614Abstract: A universal linker structure is provided, in which a functional group and activating leaving group are placed on a tether, allowing the placement of an electrophile at the end of any nucleic acid sequence. The electrophile on the tether can react with a second nucleic acid carrying a nucleophile when the two nucleic acids are hybridized near one another, resulting in release of the leaving group, and creation of a functional change. The linker can be designed to destabilize the ligation product without slowing the rate of reaction. This lowers product inhibition, and the target DNA or RNA can become a catalyst for isothermally generating multiple signals for detection. This enhanced signal is demonstrated in solution experiments and in solid supported assays. The universal linkers of the present invention are simple and inexpensive to prepare, and can be appended to any polynucleotide in automated steps on a standard DNA synthesizer.Type: GrantFiled: September 1, 2005Date of Patent: June 29, 2010Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Eric Todd Kool, Hiroshi Abe
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Publication number: 20100155590Abstract: The invention relates to a labeled transition metal complex comprising a transition metal atom, a reactive moiety for allowing a chemical or biological entity to become attached to the transition metal atom, an inert tridentate moiety as a stabilizing bridge, and a marker. The invention also relates to a labeled chemical or biological entity comprising a chemical or biological entity which is attached to said labeled transition metal complex, to the use of said complex for creating a defined shift in the molecular mass of said entity in order to facilitate mass spectrometric analysis of said entity, to methods for rendering chemical or biological entities distinguishable by mass spectrometry as well as to methods for mass spectrometric analysis of the chemical or biological entities.Type: ApplicationFiled: December 1, 2005Publication date: June 24, 2010Applicant: Kreatch Biotechnology B.V.Inventors: Marie Lacombe, Franciscus Johannes Opdam, Eduard Gerhard Talman, Jacky Theo Maria Veuskens
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Patent number: 7741044Abstract: A method of making a set of labelled compounds by the use of a preferably particulate support, comprises dividing the support into lots, performing a different chemical reaction on each lot of the support, e.g. to couple a chemical moiety to that lot of the support, tagging a fraction of each lot of the support with a different label, and combining the said lots of the support. The steps are repeated several times, preferably to build up oligomer molecules carrying labels which identify the nature and position of a monomer unit of the oligomer, and which are releasable from the support. Preferred labels, which are releasable from the compounds by cleavage to provide charged groups for analysis by mass spectrometry, are groups of the trityl (trimethylphenyl) family. Also claimed are libraries of these labels and their use in assays and nucleic acid analysis methods.Type: GrantFiled: May 4, 2007Date of Patent: June 22, 2010Inventors: Edwin Mellor Southern, Mikhail Sergeevich Shchepinov, John Nicholas Housby, Alan Lewis Hamilton, John Kenneth Elder
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Patent number: 7728128Abstract: The present invention concerns novel dibenzo[c,h][1,5]naphthyridine of formula (I) and their use as DNA probes, as well as the methods for marking DNA using the same.Type: GrantFiled: February 15, 2007Date of Patent: June 1, 2010Assignees: Institut National de la Sante et de la Recherche Medicate (INSERM), Institut Curie, Centre National de la Recherche Scientifique (C.N.R.S.), Commissariat a l'Energie AtomiqueInventors: Andreï Popov, David Grierson, Jean-Claude Florent
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Patent number: 7727716Abstract: The present invention provides methods for determining the presence of immobilized nucleic acid employing unsymmetrical cyanine dyes that are derivatives of thiazole orange, a staining solution and select fluorogenic compounds that are characterized as being essentially non-genotoxic. The methods comprise immobilizing nucleic acid, single or double stranded DNA, RNA or a combination thereof, on a solid or semi solid support, contacting the immobilized nucleic acid with an unsymmetrical cyanine dye compound and then illuminating the immobilized nucleic acid with an appropriate wavelength whereby the presence of the nucleic acid is determined. The cyanine dye compounds are typically present in an aqueous staining solution comprising the dye compound and a tris acetate or tris borate buffer wherein the solution facilitates the contact of the dye compound and the immobilized nucleic acid.Type: GrantFiled: September 30, 2004Date of Patent: June 1, 2010Assignee: Life Technologies CorporationInventors: Matthew Paul Beaudet, W. Gregory Cox, Stephen Yue
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Patent number: 7723038Abstract: Methods are provided for amplification and monitoring of oligonucleotide amplification in which a primer has an overlap with one or more bases of a detection probe.Type: GrantFiled: December 18, 2007Date of Patent: May 25, 2010Assignee: Elitech Holding B.V.Inventors: Walt Mahoney, Nicolaas M. J. Vermeulen, Irina Afonina
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Patent number: 7718374Abstract: Methods and probes are provided for the analysis of target sequences having two or more polymorphisms wherein one of the polymorphisms is to be distinguished and another polymorphism is to be masked.Type: GrantFiled: January 28, 2008Date of Patent: May 18, 2010Assignee: Elitech Holding B.V.Inventors: Yevgeniy Belousov, Robert O. Dempcy, Sergey G. Lokhov, Alexei Vorobiev
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Patent number: 7713698Abstract: This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3?-position of the deoxyribose.Type: GrantFiled: August 20, 2007Date of Patent: May 11, 2010Assignee: The Trustees of Columbia University in the City of New YorkInventors: Jingyue Ju, Zengmin Li, John Robert Edwards, Yasuhiro Itagaki
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Patent number: 7713694Abstract: A method of enhancing the fluorescence of and/or producing an increased Stokes' shift in a fluorescent dye comprising combining the dye with a base and/or a detergent is described. The method is suitable in chemical or biochemical techniques using fluorescent dyes, particularly techniques requiring staining or labeling of organic molecules, such as electrophoresis.Type: GrantFiled: September 26, 2005Date of Patent: May 11, 2010Assignee: Fluorotechnics Pty LimitedInventors: James Alexander Mackintosh, Duncan Adam Veal, Peter Helmuth Karuso, Daniel Richard Coghlan, Hung-Yoon Choi
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Publication number: 20100092971Abstract: The present invention provides, for example, a labeling substance that allows the double helix structure of a nucleic acid to be detected effectively. The present invention provides a compound having a structure derived from mononucleoside or mononucleotide, with the structure being represented by the following formula (1), (1b), or (1c), a tautomer or stereoisomer thereof, or a salt thereof. In the above formulae, B is an atomic group having a nucleobase skeleton, E is an atomic group having a deoxyribose skeleton, a ribose skeleton, or a structure derived from either one of them, or an atomic group having a peptide structure or a peptoid structure, and Z11 and Z12 each are a hydrogen atom, a protecting group, or an atomic group that exhibits fluorescence and may be identical to or different from each other.Type: ApplicationFiled: March 6, 2008Publication date: April 15, 2010Applicant: RIKENInventors: Akimitsu Okamoto, Shuji Ikeda, Takeshi Kubota
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Publication number: 20100093990Abstract: Described are modified mononucleotides and processes for their production wherein these nucleotides contain at least once the structure P?N-Acc in which Acc is an electron acceptor or an electron acceptor substituted with a residue R, and R is any organic substituent.Type: ApplicationFiled: October 16, 2009Publication date: April 15, 2010Inventor: Dieter Heindl
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Patent number: 7687618Abstract: The present invention relates to a method for the manufacture of labeled oligonucleotide conjugates comprising the reaction of (a) an oligonucleotide having a labile protecting group bound to a terminal hydroxy group, and (b) a labeling compound, wherein said labile protecting group is partially or completely substituted by said labeling compound in a nucleophilic substitution reaction.Type: GrantFiled: October 14, 2003Date of Patent: March 30, 2010Assignee: NIMBLEGEN SYSTEMS GmbHInventors: Klaus Peter Stengele, Evgueni Kvassiouk
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Patent number: 7683164Abstract: A compound which comprises a backbone having a plurality of chiral carbon atoms, the backbone bearing a plurality of ligands each being individually bound to a chiral carbon atom of the plurality of chiral carbon atoms, the ligands including one or more pair(s) of adjacent ligands each containing a moiety selected from the group consisting of a naturally occurring nucleobase and a nucleobase binding group, wherein moieties of the one or more pair(s) are directly linked to one another via a linker chain; building blocks for synthesizing the compound; and uses of the compound, particularly in antisense therapy.Type: GrantFiled: February 19, 2008Date of Patent: March 23, 2010Assignee: Bio-Rad Laboratories Inc.Inventor: David Segev