Abstract: Vessel perfusion and myocardial blush are determined by analyzing fluorescence signals obtained in a static region-of-interest (ROI) in a collection of fluorescence images of myocardial tissue. The blush value is determined from the total intensity of the intensity values of image elements located within the smallest contiguous range of image intensity values containing a predefined fraction of a total measured image intensity of all image elements within the ROI. Vessel (arterial) peak intensity is determined from image elements located within the ROI that have the smallest contiguous range of highest measured image intensity values and contain a predefined fraction of a total measured image intensity of all image elements within the ROI. Cardiac function can be established by comparing the time differential between the time of peak intensity in a blood vessel and that in a region of neighboring myocardial tissue both pre and post procedure.

Abstract: An apparatus for fluorescence molecular tomography includes an exciting light source, a carrying stage, and a receiving apparatus. The carrying stage configured to carry an subject to be examined which receives the exciting light to excite fluorescence; the receiving apparatus comprising a fluorescence detector, an exciting light detector and a beam splitter, the beam splitter is configured to allow the fluorescence to exit in a first direction and the exciting light having passed through the subject to be examined to exit in a second direction, the fluorescence detector is positioned in the first direction to the beam splitter and configured to receive and transform the fluorescence into a first electrical signal. The exciting light detector is positioned in the second direction to the beam splitter and configured to receive and transform the exciting light having passed through the subject to be examined into a second electrical signal.

Abstract: The invention relates to a material represented by the following formula (I) (M?)8(M?M??)6O24(X,X?)2:M???? formula (I). Further, the invention relates to a luminescent material, and to different medical imaging and diagnostic methods of using the material. Also disclosed is a method of securely identifying an item using the material.

Abstract: The invention provides methods and compositions for determining whether a subject containing a stent immobilized in a blood vessel has asymptomatic stent thrombosis or is at risk of developing clinically symptomatic stent thrombosis. In one approach, the method involves imaging a region of the blood vessel that contains the stent using a probe that contains a fluorochrome, for example, a near-infrared fluorochrome, and a targeting moiety that binds a molecular marker indicative of the presence of asymptomatic stent thrombosis or the development of symptomatic stent thrombosis. To the extent that the subject displays one or more such markers, the probe binds to the markers and increases the local concentration of the probe in the vicinity of the stent. The imaging method identifies those patients that display a higher density of such markers in the vicinity of the stent. As a result, those patients can be monitored for, and/or treated to prevent, symptomatic stent thrombosis.

Abstract: A medical system includes at least two sensing modules that each generate an optical signal that changes as a function of a physiological parameter of a patient. The sensing modules may be coupled to a common light source and a common receiver via an optically transmissive member. At least a first sensing module that is closest to the light source along a length of the optically transmissive member may include a waveguide to split the light emitted by the light source. A first portion of the light may be directed toward the first sensing module and a second portion of the light may be directed toward a second sensing module that is placed downstream of the first sensing module in a direction substantially along the direction of light flow through the optically transmissive member and away from the light source.

Abstract: The present invention provides an information processing apparatus capable of selecting a region of cancer cells in any tissue sample image and easily and accurately counting the number of the cancer cells. The information processing apparatus is an information processing apparatus 100 including: an acquisition unit 110 for acquiring image data obtained by reading a tissue sample image 150 obtained by putting a mark 151 specifying a selected area 152 on an image obtained by immunostaining and then imaging a biological tissue; and a counting unit 120 for counting the number of cancer cells in the selected area 152 specified by the mark 151 based on the image data of the tissue sample image 150 acquired by the acquisition unit 110, wherein a diagnosis based on the tissue sample image 150 is supported.

Abstract: Methods for imaging a tumor in an animal including labeling an organ in the animal with a first detectable label detectable by both fluorescence molecular tomography (FMT) and magnetic resonance (MR) and localized substantially exclusively to the organ, whereupon the labeled organ is capable of use as a first fiducial marker in an image of the animal; administering to the animal a detectably labeled probe that localizes substantially exclusively to a region of interest in the body of the animal; after labeling the organ and administering the detectably labeled probe, imaging the animal with a FMT imager to generate an FMT image, and imaging the animal with a MR imager to generate an MR image; and aligning the FMT and MR images using the first fiducial marker as a first reference point, thereby generating an image comprising a fusion of the FMT image and the MR image.

Abstract: Disclosed is an exemplary surgical illumination system that includes a first laser configured to emit a first light beam having a first spectral range, and an illumination probe optically connectable to the first laser. The first laser may be configured as a supercontinuum laser. The surgical illumination system may include a second laser configured to emit a second light beam having a second spectral range, and a beam combiner for combing the first and second laser beams to form a third laser beam having a spectral range of the first and second lasers. The illumination probe includes a fiber optic cable for delivering at least a portion of the first light beam to a surgical site. The fiber optic cable includes a fiber optic core having a diameter of 100 microns or less.

Abstract: Vessel perfusion and myocardial blush are determined by analyzing fluorescence signals obtained in a static region-of-interest (ROI) in a collection of fluorescence images of myocardial tissue. The blush value is determined from the total intensity of the intensity values of image elements located within the smallest contiguous range of image intensity values containing a predefined fraction of a total measured image intensity of all image elements within the ROI. Vessel (arterial) peak intensity is determined from image elements located within the ROI that have the smallest contiguous range of highest measured image intensity values and contain a predefined fraction of a total measured image intensity of all image elements within the ROI. Cardiac function can be established by comparing the time differential between the time of peak intensity in a blood vessel and that in a region of neighboring myocardial tissue both pre and post procedure.

Abstract: A micro-analytical platform for performing electrophoresis-based immunoassays was developed by integrating photopolymerized cross-linked polyacrylamide gels within a microfluidic device. The microfluidic immunoassays are performed by gel electrophoretic separation and quantifying analyte concentration based upon conventional polyacrylamide gel electrophoresis (PAGE). To retain biological activity of proteins and maintain intact immune complexes, native PAGE conditions were employed. Both direct (non-competitive) and competitive immunoassay formats are demonstrated in microchips for detecting toxins and biomarkers (cytokines, c-reactive protein) in bodily fluids (serum, saliva, oral fluids). Further, a description of gradient gels fabrication is included, in an effort to describe methods we have developed for further optimization of on-chip PAGE immunoassays.

Abstract: A micro-analytical platform for performing electrophoresis-based immunoassays was developed by integrating photopolymerized cross-linked polyacrylamide gels within a microfluidic device. The microfluidic immunoassays are performed by gel electrophoretic separation and quantifying analyte concentration based upon conventional polyacrylamide gel electrophoresis (PAGE). To retain biological activity of proteins and maintain intact immune complexes, native PAGE conditions were employed. Both direct (non-competitive) and competitive immunoassay formats are demonstrated in microchips for detecting toxins and biomarkers (cytokines, c-reactive protein) in bodily fluids (serum, saliva, oral fluids). Further, a description of gradient gels fabrication is included, in an effort to describe methods we have developed for further optimization of on-chip PAGE immunoassays.

Abstract: Packaged cardiopulmonary bypass or organ perfusion system sensor subassemblies are provided. The packaged sensor subassemblies include a sensor subassembly and a package. The sensor subassembly includes a pre-calibrated pH sensor in a dry environment, a pre-calibrated PC02 sensor in a dry environment, and a pre-calibrated pC02 sensor in a wet environment, is sterile, and is disposed in the package. Also provided are methods of use of the packaged cardiopulmonary bypass or organ perfusion system sensor subassemblies.

Abstract: Vessel perfusion and myocardial blush are determined by analyzing fluorescence signals obtained in a static region-of-interest (ROI) in a collection of fluorescence images of myocardial tissue. The blush value is determined from the total intensity of the intensity values of image elements located within the smallest contiguous range of image intensity values containing a predefined fraction of a total measured image intensity of all image elements within the ROI. Vessel (arterial) peak intensity is determined from image elements located within the ROI that have the smallest contiguous range of highest measured image intensity values and contain a predefined fraction of a total measured image intensity of all image elements within the ROI. Cardiac function can be established by comparing the time differential between the time of peak intensity in a blood vessel and that in a region of neighboring myocardial tissue both pre and post procedure.

Abstract: The present invention relates, in general, to a system for analyzing tissue perfusion using the concentration of indocyanine green and a method of measuring the perfusion rate using the system and, more particularly, to a system for measuring tissue perfusion by injecting indocyanine green into a living body, detecting variation in the concentration of indocyanine green with the passage of time, and analyzing the detected variation, and a method of measuring the perfusion rate using the system. The present invention provides a method of measuring perfusion in a living body, which enables accurate measurement for respective regions in a wide range from a perfusion rate decreased to less than 10% of normal perfusion to a perfusion rate increased to greater than normal perfusion using the above-described mechanism of ICG in a living body, which cannot be conducted using the conventional technology.

Abstract: Apparatus for searching for and detecting defects on parts that are substantially inaccessible, being located behind a wall, the apparatus comprising a first endoscope for illumination in visible light and for observation, the first endoscope and pipes for feeding and spraying penetration test substances being housed together in a rod which can be passed through an orifice in the wall in order to examine a part, the apparatus further comprising a second endoscope independent of the first endoscope and the rod for illuminating in ultraviolet light and for observing the portion of the part that has been treated by the penetration test substances.

Abstract: Apparatus for searching for and detecting defects on parts that are substantially inaccessible, being located behind a wall, the apparatus comprising a first endoscope for illumination in visible light and for observation, the first endoscope and pipes for feeding and spraying penetration test substances being housed together in a rod which can be passed through an orifice in the wall in order to examine a part, the apparatus further comprising a second endoscope independent of the first endoscope and the rod for illuminating in ultraviolet light and for observing the portion of the part that has been treated by the penetration test substances.

Abstract: The invention provides methods for visualizing perfusion or lack thereof in the spermatic cord and testicle, as well as for detecting testicular trauma. A surgical forceps adapted to facilitate such visualization is also provided.

Abstract: The invention is based, in part, on the discovery that by combining certain components one can generate a tissue-like phantom that mimics any desired tissue, is simple and inexpensive to prepare, and is stable over many weeks or months. In addition, new multi-modal imaging objects (e.g., beads) can be inserted into the phantoms to mimic tissue pathologies, such as cancer, or merely to serve as calibration standards. These objects can be imaged using one, two, or more (e.g., four) different imaging modalities (e.g., x-ray computed tomography (CT), positron emission tomography (PET), single photon emission computed tomography (SPECT), and near-infrared (NIR) fluorescence) simultaneously.

Abstract: An improved diffuse optical tomography system for in vivo non-contact imaging includes an illumination source for illuminating a specimen, a spectrum source for projecting a spectrum onto the specimen, at least one sensor configured to capture the response of the specimen to the illumination and to the projection of said spectrum.

Abstract: A method of evaluating the surface of a material that has a distinguishable infrared spectrum comprising (a) positioning an infrared fiber optic probe to be in contact with a surface of the sample or material at a region of interest for detecting attenuated total reflectance or within a sufficient distance from the surface of the region for detecting reflection, (b) detecting mid- or near-infrared radiation attenuated total reflectance or reflection off of the surface of the sample or the material, (c) analyzing the infrared radiation from step (b) for at least one of peak height, peak area, frequency and chemometric parameters, and (d) actuating the removal device when a signal from the infrared fiber optic probe is between pre-selected values for at least one of peak height, peak area, frequency and chemometric parameters for the sample of the material.

Abstract: Accurate measurement of hematocrit during blood recovery or plasma sequestration processes by means of algorithms to account for the presence of air or other non-blood substances in salvaged or separated blood is described. A method of automatic start-up of the hematocrit measurement circuit and a method of determining the minimum volume of blood required to obtain an accurate measurement of the hematocrit also is described.

Abstract: A system and method for image guided instrument targeting including a robot unit coupled with an instrument, an imaging unit, and a first control unit, which is coupled with the robot unit and coupled with the imaging unit. The control unit receives the imaging data about a target and about the instrument from the imaging unit and controls the robot unit to properly orienting the instrument for insertion, based upon the imaging data.

Abstract: An in vivo imaging device and method, the device including at least one illumination source; at least one image sensor; and at least two optical systems. The optical systems have different depths of focus. A first and second image are focused onto the image sensor.

Abstract: In an emergency medicine patient, accurate measurement of change or lack thereof from non-shock, non-ischemic, non-inflammation, non-tissue injury, non-immune dysfunction conditions is important and is provided, as practical, real-time approaches for accurately characterizing a patient's condition, using Raman (3) and/or fluorescence (30) spectroscopy with a high degree of accuracy. Measurement times are on the order of seconds. High-accuracy measurement is achieved with Raman spectroscopy interrogation of tissue. Simultaneous interrogation by NADH fluorescence spectroscopy may he used. Measurements may be non-invasive to minimally invasive. Preclinical (ultra-early) states of shock can be detected (5), severity can be determined, effectiveness of various treatments can be determined.

Abstract: This invention relates to a fluorescence-mediated molecular tomographic imaging system, designed to detect near-infrared fluorescence activation in deep tissues. The system can use targeted fluorescent molecular probes or highly sensitive activatable fluorescence molecular probes. Such probes add molecular specificity and yield high fluorescence contrast, to allow early detection and molecular target assessment of diseased tissue, such as cancers, in vivo. The new tomographic imaging system enables three-dimensional localization in deep tissues and quantitation of molecular probes.

Abstract: This invention relates to a fluorescence-mediated molecular tomographic imaging system, designed to detect near-infrared fluorescence activation in deep tissues. The system can use targeted fluorescent molecular probes or highly sensitive activatable fluorescence molecular probes. Such probes add molecular specificity and yield high fluorescence contrast, to allow early detection and molecular target assessment of diseased tissue, such as cancers, in vivo. The new tomographic imaging system enables three-dimensional localization in deep tissues and quantitation of molecular probes.

Abstract: Minimally invasive methods for measuring an analyte, such as glucose, contained in tile interstitial fluid of a body are provided. The methods include the steps of. (a) providing at least one sensor particle capable of generating a detectable analyte signal in responding to the analyte concentration of the body, (b) placing the sensor particle into the skin of the body for allowing the sensor particle to be in contact with the interstitial fluid of the body to generate the detectable analyte signal, (c) detecting the generated analyte signal, and (d) determining the concentration of the analyte contained in the interstitial fluid. The sensor particles may be made to be responsive to an analyte such as glucose concentration contained in a body fluid by including a photo-induced electron transfer receptor specific for the analyte in the sensor particle.

Abstract: The invention relates to conjugates, comprising a fluorescent compound and a carrier, wherein the compound and the carrier are connected via an acidic ester or an acidic amide bond and the compound has an excitation wavelength of 630 nm or more and/or 450 nm or less. The invention also relates to the production of said conjugates and to the use thereof.

Abstract: An optically based transcutaneous blood gas sensor is provided having a reusable sensor head and a disposable sensing cap. A separate receptacle detachably connects to the sensor head and is attached to a person's skin by a layer of adhesive after a release liner is removed. The sensor head includes a sealed cavity housing one or more LEDs and one or more photodetectors. The disposable cap carries fluorescent targets which produces an amount of fluorescence as a function of blood gas concentration and the fluorescence is sensed by the photodetectors.

Abstract: A disposable high density optically readable polydeoxynucleotide array with integral fluorescence excitation and fluorescence emission channels is described. The compact array size allows integration into several types of interventional devices such as catheters, guidewires, needles, trocars and may be used intraoperatively. Highly sensitive monitoring of the metabolic and disease pathways of cells in vivo under varying chemical, genetic and environmental conditions is afforded.

Abstract: A method for using acetic acid as a signal enhancing contrast agent during fluorescence spectroscopy of normal and neoplastic tissue, particularly epithelium. In one aspect, the invention includes a method of detecting tissue abnormality in a diagnostic tissue sample in a patient, comprising the steps of obtaining a first fluorescence intensity spectrum from the diagnostic tissue sample; thereafter, applying acetic acid to the diagnostic tissue sample in sufficient concentration to alter the response of such diagnostic tissue sample to electromagnetic radiation for at least an effective period of time; during the effective period of time, obtaining a second fluorescence intensity spectrum from the diagnostic tissue sample; determining a parameter indicative of a change between the first and second fluorescence emission intensity spectra; and analyzing the determined parameter to determine a probability that the diagnostic tissue sample is normal or abnormal.

Abstract: A method for the determination of liver function, comprises the steps of:(i)introducing an effective amount of a coloured or fluorescent bile acid derivative intravenously into a patient,(ii)collecting samples of blood which has passed through the liver of the patient at timed intervals after step (i), and(iii)assessing the colour or fluorescence of bile acid derivative in each sample.