Abstract: This invention describes the administration of multiple therapeutic agents with insulin in conjunction with bexarotene, ketamine, monoclonal antibodies Etanercept, IGF-1, and acetylcholine esterase inhibitors physostigmine, for treatment of Alzheimer's disease and other neurodegenerative diseases. Insulin, improves memory; also augments and amplifies the effects of the adjuvant therapeutic agents (paracrine and intracrine effects) and consequently reduces the ? amyloid, its soluble precursors, prevents damage to the neuronal skeletal network (taupathy), and blocks glutamate excitotoxicity, reduces brain inflammation, prevents apoptosis, and increases the acetylcholine levels in the neurons and synapses; by using a combination of insulin, bexarotene, ketamine, Etanercept, IGF-1, and physostigmine therapeutic agents.

Abstract: The device disclosed herein may be affixed over a transparent dressing overlying the catheter entry point, or may be used independent of such a dressing. The device may be affixed to the catheter insertion site using an adhesive or fastening band, or through other methods. Depending on context, the device may be used continuously or periodically, and may be affixed such that it is directly abutting a transparent dressing or directly adjacent to the skin surface, or it may be spaced a certain distance from the skin. If spaced from the skin, light may be directed from the light sources, either directly or indirectly through optical conduits. Preferably the light sources will be positioned such that light from the light sources overlaps at one or more areas on the skin, especially the catheter entry point.

Abstract: An object of the invention is to provide a method and apparatus for the delivery of polynucleotide vaccines into mammalian skin cells to increase T cell response and to reduce pain and discomfort due to long electric waveform application and due to muscle contractions. The method for the delivery of polynucleotide vaccines into mammalian skin cells includes the steps of: (a.) administering a polynucleotide vaccine into the skin at an administration site, (b.) applying a needle electrode to the skin in the vicinity to the administration site, and (c.) applying a sequence of at least three single, operator-controlled, independently programmed, narrow interval electrical waveforms, which have pulse intervals that are less than 100 milliseconds, to deliver the polynucleotide vaccine into the skin cells by electroporation.

Abstract: Embodiments of dermalogical cell treatment are described generally herein. Other embodiments may be described and claimed.

Abstract: This invention describes the administration of multiple therapeutic agents with insulin in conjunction with bexarotene, ketamine, monoclonal antibodies Etanercept, IGF-1, and acetylcholine esterase inhibitors physostigmine, for treatment of Alzheimer's disease and other neurodegenerative diseases. Insulin, improves memory; also augments and amplifies the effects of the adjuvant therapeutic agents (paracrine and intracrine effects) and consequently reduces the ? amyloid, its soluble precursors, prevents damage to the neuronal skeletal network (taupathy), and blocks glutamate excitotoxicity, reduces brain inflammation, prevents apoptosis, and increases the acetylcholine levels in the neurons and synapses; by using a combination of insulin, bexarotene, ketamine, Etanercept, IGF-1, and physostigmine therapeutic agents.

Abstract: A needle for an endoscope, includes: an outer sheath tube having a fluid passage provided therein; a distal end discharging portion that is accommodated in the distal end of the outer sheath tube so as to be extended therefrom and retracted thereinto, and is connected to the distal end of the fluid passage; an operating device for operating the distal end discharging portion so as to be extended from the distal end of the outer sheath tube and retracted thereinto; a fluid supplying device, that is provided in the outer sheath tube and is caused to communicate with the fluid passage, and supplies a fluid to the distal end discharging portion through the fluid passage; and a blocking device that is provided in the fluid passage, for freely opening and shutting off circulation of a fluid in line with an operation of the operating device.

Abstract: Methods for treating a patient using therapeutic spinal neuromodulation and associated devices, systems, and methods are disclosed herein. One aspect of the present technology is directed to methods including modulating nerves of one or more targeted organs proximate one or more dorsal root ganglia, stellate ganglia, vertebral ganglia, or cervical ganglia of the nerves using an intravascularly-positioned therapeutic element. One or more measurable physiological parameters corresponding to at least one condition associated with sympathetic activity in the targeted organs and/or central sympathetic activity in the patient can thereby be reduced.

Abstract: A drug-delivery device includes a body configured for attachment to a handle of an ablation device, a shaft portion defining a passageway therein, and a delivery lumen to provide for drug delivery to tissue. The body includes a proximal portion, a distal portion, and a contoured portion disposed therebetween. The contoured portion is configured for engagement with a contoured portion of the handle of the ablation device. The shaft portion includes a proximal end and a distal end. The proximal end of the shaft engages with an opening defined in the distal end of the body. The passageway of the shaft portion is configured to receive the delivery lumen slideably moveably therein. The delivery lumen includes a proximal portion and a distal portion. The drug-delivery device also includes a knob member coupled to the proximal portion of the delivery lumen.

Abstract: The perivascular leak repair system, and method of using the same, of the present invention provides a sealant reservoir 102 with a repair catheter 104 operably attached; a flow control device 106 disposed between the sealant reservoir 102 and the repair catheter 104, and the flow control device 106 responsive to a flow control signal 108; a heart phase detector 114 generating a diastole phase signal 112; an injection switch 122 generating a injection signal 120; and a flow controller 110 responsive to the diastole phase signal 112 and the injection signal 120, and generating the flow control signal 108. A method of sealing a perivascular leak comprises identifying the perivascular leak 140; inserting a repair catheter to the perivascular leak 142; injecting sealant at the perivascular leak 144; and removing the repair catheter 146. The sealant can be injected when the heart is in diastole to sweep the sealant into the perivascular leak.

Abstract: A multi-pole synchronous pulmonary artery radiofrequency ablation catheter, wherein an adjustment apparatus is arranged on a control handle; a catheter body is hollow, and a cavity is arranged therein; a lead wire, a temperature sensing wire and a pull wire are arranged in the cavity; one end of the catheter body is flexible, and the flexible end is connected to an annular ring; the other end of the catheter body is connected to the control handle; one end of the pull wire is connected to the flexible end, and the other end of the pull wire is connected to the adjustment apparatus on the control handle, the tension of the pull wire is adjusted through the adjustment apparatus to achieve radian control of the flexible end; a shape memory wire is arranged in the annular ring, one end of the shape memory wire extends to the end of the annular ring and the other end of the shape memory wire passes through the root of the annular ring and is fixed on the flexible end of the catheter body; the annular ring is provi

Abstract: An apparatus for generating cut surfaces in the cornea of an eye in order to correct ametropia is provided, said apparatus comprising a laser unit, which can focus pulsed laser radiation into the cornea and move it therein in order to generate cut surfaces, and a control unit, which controls the laser unit for generating cut surfaces such that a predetermined lenticle to be removed is separated from the surrounding corneal material in the cornea by at least one cut surface, and that at least two mutually spaced apart cut surfaces are formed as opening cuts, each extending from the lenticle to the anterior corneal surface, the position and shape of the opening cuts being selected such that the opening cuts contribute to the correction of the ametropia of the eye or do not counteract the correction of the ametropia of the eye.

Abstract: A medical microelectrode includes an elongate electrode body including a tip section, a main body section and, optionally, a coupling section. The tip section, the main body section and, optionally, the coupling section are embedded in a first electrode matrix element, which is substantially rigid, biocompatible and soluble or biodegradable in a body fluid. Additionally the microelectrode includes a dissolution retarding layer on the first electrode matrix element and/or a second electrode matrix element disposed between the first electrode matrix element and the electrode. Upon dissolution or biodegradation of the first electrode matrix element a drug comprised by the first electrode matrix element or the second electrode matrix element is released into the tissue. Also disclosed are bundles and arrays of the electrodes and their use.

Abstract: A method of performing a medical procedure is disclosed. The method includes: positioning an energy delivery device configured to deliver energy adjacent uric acid (UA) crystals having a resonance frequency; applying energy to tissue through the energy delivery device at a predetermined frequency thereby resonating the UA crystals; debulking at least a portion of the UA crystals; and removing at least a portion of the debulked UA crystals.

Abstract: Methods and systems for laser assisted delivery of therapeutic agents include preparing a site with an ultraviolet laser beam, at a wavelength appropriate for tissue ablation, such that an opening is produced in a surface of the site's tissue; and applying one or more agents to the prepared site, such that the agents penetrate the tissue through the opening to a predetermined depth.

Abstract: Systems for laser assisted delivery of therapeutic agents include an ultraviolet laser configured to produce a laser beam at a wavelength appropriate for tissue ablation; a lens configured to focus and direct the laser beam to a site, such that an opening is produced in a surface of the site's tissue; a nozzle having an aperture that emits the laser beam and that controls delivery of one or more agents to the site; and an imaging device configured to monitor a depth of penetration of the one or more agents into the site's tissue.

Abstract: Photodynamic devices for stabilizing a joint are provided. In some embodiments, a photodynamic joint spacer is provided that includes an expandable member configured for attachment to a bone having an excised region; a formable bar configured for attachment to the bone along at least a portion of the expandable member; and a light-sensitive liquid passed into the expandable member to expand the expandable member, wherein the expandable member is shaped by the formable bar to a shape resembling the excised region of the bone, and wherein the light-sensitive liquid is curable upon exposure to light energy to set the expandable member in the shape resembling the excised region to form a photodynamic joint spacer.

Abstract: Systems and methods for illumination of a vascular space and its contents using an electromagnetic energy source that supplies a plurality of wavelengths ranging from ultraviolet to infrared are shown. Illumination can be performed using multiple wavelengths, simultaneously or sequentially, and can be performed in accordance with a protocol where an initial illumination produces an effect that is at least partially reversed by a subsequent illumination. Illumination protocols can be stored on a database and accessed via a user interface displayed on the electromagnetic energy source. The database can be used to store data related to performance of system components, and such data can be used to override or modify an illumination protocol.

Abstract: A lacrimal drainage manometer includes a syringe having a syringe body and a piston. The syringe body defines a fluid cavity in fluid communication with a cannula configured for insertion into at least a portion of a lacrimal drainage system. The piston dispenses a fluid from the fluid cavity through the cannula. A pressure sensor is operably coupled to the syringe for measuring fluid pressure in the fluid cavity. A position sensor operably coupled to the syringe measures the position of the piston relative to the syringe body. A user feedback unit in electrical communication with the pressure sensor and the position sensor provides user feedback based on data from at least one of the pressure sensor and the position sensor indicative of a condition of the lacrimal drainage system.

Abstract: The current invention provides this advance in infection control via its unique application of active sterilization to a catheter or implant. While most catheters, and many implants, are passive devices, the current invention will provide an active component as a integral part of the implanted catheter or device to continuously or intermittently sterilize the exposed surfaces/areas of the device. This active sterilization may be accomplished by a variety of mechanisms, including, application of heat, RF, microwave, ultrasound, ultraviolet radiation or other energy capable of sterilizing the device or dislodging any problematic Biofilm that may form. The active sterilization may also employ the pumping of a sterilizing chemical from all attached drug reservoir, the use of electricity or freezing temperatures or any other mechanism for either inhibiting, killing or dislodging any infectious material in contact with the implant.

Abstract: Apparatus and method for ablating target tissue including a non-linear area of tissue in the left atrium of a patient. The method can include selecting an ablation apparatus having an ablator with a tissue engagement section, penetrating a chest cavity of the patient, and identifying the target tissue. The method can also include positioning the ablation apparatus adjacent to the target tissue so that the tissue engagement section can transfer ablation energy to the target tissue. The method can further include energizing the tissue engagement section with ablation energy in order to create a footprint on the non-linear area of tissue in the left atrium and to reduce an overall mass of excitable tissue in the left atrium.

Abstract: The filling status of a drug reservoir in a drug pump devices may be determined with mechanical and/or magnetic position sensor associated with a reservoir boundary.

Abstract: A catheter and methods for luminal therapy are provided wherein a catheter has an outer balloon with a multiplicity of apertures for infusing one or more therapeutic agents into a vessel wall, an intermediate balloon having a multiplicity of apertures offset from the apertures of outer balloon to serve as a baffle that reduces jetting and promotes uniform distribution of therapeutic agent exiting through the outer balloon, and an impermeable inner balloon disposed within the intermediate balloon that enables the intermediate and outer balloons to be forced into engagement with the vessel wall to dilate the vessel and disrupt plaque lining the vessel wall and to also facilitate the uniform delivery of the therapeutic agent.

Abstract: An apparatus for treating solid tumors having at least one hollow needle having at least one perforation, at least one ground electrode, a pump for delivering a chemotherapeutic agent through the perforation in the hollow needle, one or more power supplies connected to the hollow needle and the ground electrode capable of delivering a pulse of alternating current between the hollow needle and the ground electrode of at least 1000 volts and no more than 1 millisecond duration, a direct current between the hollow needle and the ground electrode between about 1 mA and 100 mA, and a second alternating current having a frequency range between about 1 MHz and 1000 MHz.

Abstract: A nerve block catheter system employs an indwelling, flexible catheter comprising a tissue lock to retain the catheter tip in pharmacologically proximity to a target nerve and optionally, a decoupler that insulates the tip of the catheter from proximal tissue movement.

Abstract: The present invention provides improved methods and apparatus for skin treatment and tissue remodeling. The apparatus includes an array of needles that penetrate the skin and serve as electrodes to deliver radio frequency current or other electrical or optical energy into the tissue being treated, causing thermal damage in controlled patterns. The damaged regions promote beneficial results such as uniform skin tightening by stimulation of wound healing and collagen growth.

Abstract: A dynamic electroporation appliance comprises an oblong isolating applicator (1) with a rounded front part (4) and with two annular electrodes (3) arranged on its front surface at the sides of an intermediate opening (2). The intermediate opening it linked to at least one duct (9) associated with a central seat for a syringe (6) for dispensing pharmacological or aesthetic products or active ingredients. The electrodes (3) are energizable to produce a dynamic electroporation effect on the treated surface.

Abstract: An intraluminal activation system for activating an inactive agent includes a catheter defining an inflation lumen and a medical device lumen. A longitudinal distal segment of the catheter defines a transparent treatment region. An inflation balloon is disposed at the distal end of the catheter and over at least a portion of the transparent treatment region. An elongate energy transmission wire is configured to transmit activation energy from a proximal end thereof to a distal end thereof. The activation system has a configuration in which the balloon is expanded, the energy transmission wire is received within the medical device lumen such that the distal end of the energy transmission wire is within the transparent treatment region, and activation energy is emitted from the energy transmission wire, through the transparent treatment region, through the balloon, and toward the inactive agent.

Abstract: A device for treating the skin comprises a handpiece assembly having a distal end and a proximal end and a tip on the distal end of the handpiece assembly. The handpiece assembly includes a fluid delivery conduit, a waste conduit and/or an energy delivery conduit. Further, the tip is configured to contact the skin. The tip comprises a peripheral lip and a plurality of needles generally positioned within the peripheral lip. The tip also comprises at least one opening in fluid communication with a fluid delivery conduit, at least one opening in fluid communication with a waste conduit and/or an energy contact point in electrical communication with an energy contact point and energy conduit in the main body portion. The plurality of needles may be movable with respect to the peripheral lip through the use of a pneumatic or other force.

Abstract: Methods and devices for diagnosing, monitoring, and/or treating tissue through an opening or port through an airway wall. A passageway is created extending from the airway to the target tissue. The passageway provides local access to the target tissue for treatment instruments and for delivering agents.

Abstract: A device and method for delivering a drug from inside a body lumen to tissue surrounding the body lumen. An endoluminal drug delivery device includes a dual-lumen catheter for housing a guidewire and a needle connectable to a drug source. The guidewire exits the catheter through an opening at the distal end, and the needle exits the catheter through an exit port in the outer wall of the catheter. A distal portion of the catheter has a single lumen and includes a taper, allowing the distal tip to act as a dilator. The device optionally includes a catheter lumen splitter and/or a handpiece assembly. A method of delivering fluid to tissue surrounding a body lumen includes inserting a guidewire into the body lumen, tracking the device over the guidewire, deploying the needle through the exit port to the tissue, delivering fluid, and retracting the needle into the catheter.

Abstract: A method and device are presented for providing patient safe light to a wound. The device includes a radiation source producing flora lethal radiation wavelengths, a radiation conduit detachably optically coupled to the radiation source, and a patch remotely located from the radiation source configured to at least partially conform to a surface contour of the wound. The patch includes a flexible panel formed of a radiation transmitting material able to withstand sterilization, including at least one surface with a disturbed surface area configured to emit radiation upon the wound.

Abstract: A procedure and kit are provided for performing an ultrasound-guided transversus abdominis plane (TAP) procedure. The patient's abdomen is scanned with an ultrasound probe to identify and mark the external oblique, internal oblique, and TAP. An introducer sheath is placed over a fluid delivery needle such that the distal end of the needle extends beyond the distal end of the sheath, the needle having echogenic properties for ultrasound imaging. The needle and sheath are ultrasonically guided into the TAP. A local anesthetic or saline/anesthetic combination is injected through the needle to create a liquid pool in the TAP. The needle is removed from the sheath while maintaining the sheath within the TAP and a catheter is subsequently advanced through the sheath and into the pooled liquid in the TAP. The sheath is withdrawn while maintaining the catheter located within the TAP.

Abstract: A device to stimulate a scalp comprising an array of stimulating elements adapted to pierce the skin of the scalp no deeper than a thickness of a dermis, wherein the stimulating elements are arranged along a circumference of at least one wheel and the wheel is adapted to roll over the scalp.

Abstract: Described are hemostasis devices useful for implantation in biopsy tracts, which device comprise highly compact, dried hemostatic sponge elements. Preferred devices also include compacted sponge matrices exhibiting high density and rigidity in combination with high volumetric expandability when wetted. Also described are methods for making and using such devices.

Abstract: Devices and methods for delivering substances to lung tissue through an extra-anatomic passage created in an airway.

Abstract: The present invention relates to an injection device comprising: —a first support (14) having a cup-shaped first contact surface (18) intented to come into contact with a first region of an outside surface of an eye, —a set of at least four injection needles (17) in fluid communication with each other and protruding from said first contact surface (18) at respective insertion points (22) so that the distance between the distal end (26) of any of said injection needles to said first contact surface is between 0.6 mm and 1.3 mm, the insertion points of the injection needles on the first contact surface being spread on said first contact surface so that the diameter (D?) of the largest circle (C?) that it is possible to include completely in the convex surface (E) defined by said insertion points on a front view of said first contact surface, without any insertion point being included in said circle, is less than 8 mm.

Abstract: The invention generally relates to a continuous anesthesia nerve conduction apparatus and method thereof, and more particularly to a method and system for use in administering a continuous flow or intermittent bolus of anesthetic agent to facilitate a continuous or prolonged nerve block. In one embodiment, the apparatus includes a sheath having a proximal end, a distal end and at least one lumen extending from the proximal end to the distal end. The sheath also includes an embedded conductive element for transmitting an electrical signal from a proximal portion of the sheath to a distal portion of the sheath. A cannula is arranged in the at least one lumen of the sheath and has a distal end protruding from a distal portion of the sheath. The cannula is electrically coupled to at least a portion of the embedded conductive element and is configured to provide nerve stimulation.

Abstract: The invention generally relates to a continuous anesthesia nerve conduction apparatus and method thereof, and more particularly to a method and system for use in administering a continuous flow or intermittent bolus of anesthetic agent to facilitate a continuous or prolonged nerve block. In one embodiment, the apparatus includes a sheath having a proximal end, a distal end and at least one lumen extending from the proximal end to the distal end. The sheath also includes an embedded conductive element for transmitting an electrical signal from a proximal portion of the sheath to a distal portion of the sheath. A cannula is arranged in the at least one lumen of the sheath and has a distal end protruding from a distal portion of the sheath. The cannula is electrically coupled to at least a portion of the embedded conductive element and is configured to provide nerve stimulation.

Abstract: A catheter includes a braid assembly having a dual-laminate coating. The braid assembly includes a plurality of braid members interwoven to provide for interstices between the braid members, each braid member having an electrically conductive element, a flexible non-electrically-conductive polymer coating that insulates the electrically conductive element and a thermoplastic bonding adhesive coating. The braid assembly is formed between an inner polymer layer and an outer polymer layer. One or more of the braid members may be coupled to an energy delivery element.

Abstract: An apparatus for treating the brain, comprises a first fluid delivery device including a distal end sized and shaped for placement at a first target site between a dura mater and a pia mater of the brain, the first fluid delivery device including a first fluid lumen extending to a first outlet port in the distal end to deliver fluids to a first target location and a first microelectrode mounted within the distal end of the first fluid delivery device for movement between an insertion position in which a first distal tip of the first microelectrode is received within the first fluid delivery device and a deployed position in which the first microelectrode extends out of the first fluid delivery device with the first distal tip thereof penetrating the pia mater to a first electrode target position in the cerebral cortex.

Abstract: Restenosis or neointimal formation may occur following angioplasty or other trauma to an artery such as by-pass surgery. This presents a major clinical problem which narrows the artery. The invention provides a balloon catheter with a particular electrode configuration. Also provided is a method whereby vascular cells in the area of the artery subjected to the trauma are subjected to irreversible electroporation which is a non-thermal, non-pharmaceutical method of applying electrical pulses to the cells so that substantially all of the cells in the area are ablated while leaving the structure of the vessel in place and substantially unharmed due to the non-thermal nature of the procedure.

Abstract: A catheter suitable for delivering an electric current to the body, in particular a catheter having electrodes that can be positioned independently of the main elongate shaft of the catheter. More particularly the catheters include a movable sleeve that incorporates the electrodes. A movable sleeve includes one or more electrodes and advances in the construction of the electrodes and related components is disclosed. Methods for positioning electrodes at a treatment site in the body for diagnostic or therapeutic applications, particularly electrical pharyngeal stimulation are also disclosed.

Abstract: A neural probe (10) is provided for in vivo communication with biological tissue, including stimulating neurons and/or recording neural electrical activity. The probe (10) may be constructed so that the immune response by the biological tissue in which the device is implanted is reduced over known implantable probes. The probe (10) can be constructed with a tip (16) that has a branched configuration, with electrodes (24) located along each of the branches (20). A biodegradable coating (18) is disposed over at least the tip (16) of the probe (10) to provide the probe (10) with sufficient integrity for insertion into the biological tissue and to degrade after insertion. The biodegradable coating (18) can have an anti-inflammatory drug or other bioagent distributed therein for localized release of the bioagent to further reduce the immune response.

Abstract: A system and method for use in iontophoretic anesthesia of a tympanic membrane are disclosed. The system generally includes an earplug and an electrode device. The earplug includes at least one sealing member for sealing the earplug in an ear canal. The sealing member includes microholes which vent fluid above a certain pressure threshold. A headset may connect the earplug to a second earplug. The method involves using the system on a human or animal subject.

Abstract: A method and an apparatus of applying carboxytherapy to a subject is disclosed. The method comprises the steps of: contacting a target body surface of the subject with a treatment device comprising a plurality of hollow needles attached to a contact surface of a housing and a CO2 source in fluid communication with at least one of the plurality of hollow needles; applying pressure to the housing such that one or more of the plurality of hollow needles penetrate an epidermis or an outermost layer of cell in the target body surface; applying a therapeutic amount of CO2 to the subject through said plurality of hollow needles; and removing the plurality of hollow needles from said target body surface. A method of applying carboxytherapy to improve skin conditions is also disclosed.

Abstract: A method for treating skin in a subject is disclosed. The method comprises the steps of administering a stem cell composition into a target body site in the subject by subcutaneous, intradermal or topical delivery, and contacting the target body site with a treatment device designed for introducing CO2 into subcutaneous tissue, wherein the CO2 and the stem cell composition are provided in an amount effective for treating a skin-related disorder or improving the condition of skin. Also disclosed is a method for generating induced pluripotent stem cells (iPSCs) in a subject.

Abstract: A glaucoma treatment system includes: a cannula body configured to be positioned in an area of Schlemm's canal; an illumination guide extending along the cannula body; at least one drug source coupled to the cannula body; a cross-linking agent source coupled to the cannula body; and an illumination source coupled to the illumination guide. The at least one drug source includes a drug that promotes outflow of aqueous humor through the trabecular meshwork and into Schlemm's canal. The cannula body delivers the drug from the at least one drug source to the area of Schlemm's canal, and in response to changes in the outflow of aqueous humor, delivers the cross-linking agent to the area of Schlemm's canal. The illumination guide delivers photo-activating light from the illumination source to the area of Schlemm's canal. The photo-activating light activates the cross-linking agent, thereby stabilizing changes in the area of Schlemm's canal.

Abstract: Balloon catheter configured to deliver a therapeutic agent upon provision of electric potential to the balloon, the catheter comprising an electrode disposed proximate the outer surface of the expandable member, a coating disposed on at least a portion of the outer surface, the coating including a therapeutic agent; and a power source in electrical communication with the electrode is described.

Abstract: An applicator for use in tissue repair is provided. The applicator includes a base configured to dispense a material and an applicator tip operably connected to the base. The applicator tip includes a body having a distal end configured to engage a tissue defect and a mold disposed about the body configured to engage tissue about the tissue defect. The mold is configured to be selectively positioned about the distal end of the body between at least a first position and a second position to deposit material to form at least a first layer and a second layer. Also provided are applicator tips, methods and kits for tissue repair.

Abstract: Electromotive delivery of macromolecules can be provided using a delivery device including a first chamber for contacting a macromolecule delivery medium to a surface of a tissue or other anatomy of a patient; and a second chamber for contacting a receiving medium to the patient. A first electrode can be disposed in the first chamber so as to not directly contact the macromolecule delivery medium. A second medium having a buffering agent can be used to keep the first electrode from coming into direct contact with the macromolecule delivery medium. A second electrode can be disposed within the receiving medium in the second chamber such that the second electrode does not directly contact the patient. An electric field can be generated using the first and second electrodes in order to cause the macromolecule delivery medium to move into the tissue of interest from the first chamber of the delivery device.