Abstract: Disclosed herein are methods and compositions for inactivating TCR and/or HLA genes, using engineered nucleases comprising at least one DNA binding domain and a cleavage domain or cleavage half-domain in conditions able to preserve cell viability. Polynucleotides encoding nucleases, vectors comprising polynucleotides encoding nucleases and cells comprising polynucleotides encoding nucleases and/or cells comprising nucleases are also provided.

Abstract: New insecticidal proteins, nucleotides, peptides, their expression in plants, methods of producing the peptides, new processes, production techniques, new peptides, new formulations, and new organisms, a process which increases the insecticidal peptide production yield from yeast expression systems. The present invention is also related and discloses selected endotoxins we call cysteine rich insecticidal peptides (CRIPS) which are peptides derived from Bacillus thuringiensis (Bt) and their genes and endotoxins in combination with toxic peptides known as Inhibitor Cystine Knot (ICK) genes and peptides as well as with other types of insecticidal peptides such as trypsin modulating oostatic factor (TMOF) peptide sequences used in various formulations and combinations; of both genes and peptides, useful for the control of insects.

Abstract: The present disclosure describes methods of obtaining and/or deriving proteins from plants of the Nicotiana species and methods for incorporation of such proteins into various products. For example, a method for obtaining a protein-enriched material from a plant of the Nicotiana species or portion thereof is provided, comprising: extracting one or more proteins from the plant material into a solvent to form a liquid protein-containing extract; separating a solid extracted plant material from the liquid protein-containing extract; clarifying the liquid protein-containing extract to form a clarified protein-containing extract and a solids fraction; and treating the clarified protein-containing extract so as to provide a protein-enriched material comprising at least about 60% protein by weight.

Abstract: A soybean cultivar designated 81171312 is disclosed. The invention relates to the seeds of soybean cultivar 81171312, to the plants of soybean cultivar 81171312, to the plant parts of soybean cultivar 81171312, and to methods for producing progeny of soybean cultivar 81171312. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar 81171312. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar 81171312, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar 81171312 with another soybean cultivar.

Abstract: Molecular markers are described for determining the presence or absence of a gene conferring resistance to tomato yellow leaf curl virus from S. chilense (Ty-1) in a host plant. Also described, are methods for producing a host plant comprising a gene conferring resistance to tomato yellow leaf curl virus from S. chilense (Ty-1), including the analysis of the presence or absence of the molecular markers. A plant, and parts thereof, obtained by such a method are also described.

Abstract: Disclosed herein are viral vectors based on modifications of the Citrus Tristeza virus useful for transfecting citrus trees for beneficial purposes. Included in the disclosure are viral vectors including one or more gene cassettes that encode heterologous polypeptides. The gene cassettes are positioned at desirable locations on the viral genome so as to enable expression while preserving functionality of the virus. Also disclosed are methods of transfecting plants and plants transfected with viral vector embodiments.

Abstract: Described herein are viral amplicon-based protein expression systems and methods useful for producing heterologous proteins, such as enzymes, by agroinfiltration. The methods involve producing an Agrobacterium with a Ti plasmid encoding a heterologous protein, infecting plant cells with the Agrobacterium, allowing expression of the heterologous protein, and recovering the heterologous protein from the plant cells. In one embodiment, the protein produced is an endoglucanase.

Abstract: The present invention provides means and methods for simple and efficient introduction of foreign genetic material into the plant cell. Particularly, the present invention combines seed priming and virus-based DNA constructs for efficient introduction of heterologous DNA into plants.

Abstract: Disclosed herein are viral vectors based on modifications of the Citrus Tristeza virus useful for transfecting citrus trees for beneficial purposes. Included in the disclosure are viral vectors including one or more gene cassettes that encode spinach defensin peptide(s). The gene cassettes are positioned at desirable locations on the viral genome so as to enable expression while preserving functionality of the virus. Also disclosed are methods of transfecting plants and plants transfected with viral vector embodiments.

Abstract: The present invention relates to the field of functional analysis of Jatropha genes on a genomic scale. More specifically, the present invention relates to a method for high-throughtput functional analysis of Jatropha curcas genes on a genomic scale using virus-induced gene silencing. The method involves use of the tobacco rattle virus (TRV).

Abstract: A soybean cultivar designated S110126 is disclosed. The invention relates to the seeds of soybean cultivar S110126, to the plants of soybean cultivar S110126, to the plant parts of soybean cultivar S110126, and to methods for producing progeny of soybean cultivar S110126. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar S110126. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar S110126, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar S110126 with another soybean cultivar.

Abstract: A method of generating genotypic variation in a genome of a plant is disclosed. The method comprising introducing into the plant at least one viral expression vector encoding at least one chimeric nuclease which comprises a DNA binding domain, a nuclease and a localization signal to a DNA-containing organelle, wherein the DNA binding domain mediates specific targeting of the nuclease to the genome of the plant, thereby generating genotypic variation in the genome of the plant.

Abstract: A method of producing a lunasin polypeptide in a plant includes expressing a fusion protein including the lunasin polypeptide in the plant and cleaving the lunasin polypeptide from the fusion protein.

Abstract: Methods of expressing a molecule of interest in a plant are disclosed. One method comprises contacting roots of the plant in a solution comprising at least one Geminivirus based expression construct so as to allow the at least one Geminivirus based expression construct to be absorbed by the roots, the expression construct comprising a polynucleotide encoding the molecule of interest, and further the expression construct being capable of systemic symptomless spread in a plant host, thereby expressing a molecule of interest in a plant. Expression constructs capable of systemic symptomless spread in a host plant are also disclosed.

Abstract: The present invention relates to nucleic acid molecules useful for conferring broad and durable resistance to grapevine fanleaf virus in plants. The invention also relates to methods of enhancing resistance to plant pathogens and plants or plant components (such as grape plants) expressing such nucleic acid molecules. In addition, the invention relates to products (e.g., foodstuffs including beverages such as wine or juice) derived from grape plants transformed with such nucleic acids.

Abstract: We have developed a versatile plant viral vector system based on Alternanthera mosaic virus (AltMV), suitable for infection by agroinfiltration or in vivo T7 transcripts from the same clone; agroinfection is enhanced by coinfiltration of a T7 RNA polymerase construct. Variants adapted for efficient protein expression, or for virus-induced gene silencing (VIGS), are based on a specific amino acid substitution (L88P) in the triple gene block 1 (TGB1) protein affecting RNA silencing suppression. A bipartite delivery system developed for AltMV delivers replicase (RdRp) functions separately from movement and encapsidation (TGB and coat protein, CP) functions by agroinfiltration, resulting in precise recombination of RdRp and TGB-CP constructs in planta. The bipartite delivery system has potential for high throughput protein expression or VIGS with the appropriate TGB1 variant, for hosts including Nicotiana benthamiana and Arabidopsis thaliana.

Abstract: A process of producing one or more than one protein of interest, comprising: (a) providing a plant or plant cells comprising a first heterologous nucleotide sequence comprising a nucleotide sequence encoding an RNA replicon, and a first inducible promoter operably linked to said nucleotide sequence encoding said RNA replicon; said RNA replicon not encoding a protein providing for cell-to-cell movement of said RNA replicon in said plant; said RNA replicon encoding a polymerase and said one or more than one protein of interest, said polymerase being adapted for replicating said RNA replicon; and (b) inducing, in said plant or plant cells of step (a), said inducible promoter, thereby producing said one or more than one protein of interest in said plant or plant cells.

Abstract: A process for replicating or for replicating and expressing a sequence of interest in a plant, comprising: (i) an RNA replicon or a precursor thereof, said RNA replicon being derived from a plus-sense single stranded RNA virus and comprising at least one sequence of interest; and (ii) a helper replicon, or a precursor thereof, wherein said helper replicon is (a) incapable of systemic movement in said plant both in the presence and in the absence of said RNA replicon (i) and (b) capable of expressing in a plant one or more proteins necessary for systemic movement of said RNA replicon (i), whereby said RNA replicon (i) is capable of replicating or replicating and expressing said sequence of interest in said plant, but unable to move systemically in said plant in the absence of said one or more proteins expressed by said helper replicon (ii).

Abstract: It is intended to provide a polynucleotide comprising a viral base sequence, the viral base sequence containing: a first base sequence encoding a viral replication protein, and a second base sequence encoding a viral movement protein, the second base sequence being located downstream of the first base sequence and having a linking site for linking with an exogenous base sequence encoding a polypeptide to be expressed, the linking site being located downstream of the second base sequence, the second base sequence being obtained by modifying with a base sequence in a native sequence derived from a virus by insertion, substitution, or addition. By using this, a vector containing a viral base sequence is constructed, and a protein is efficiently produced without worsening growth of a host cell containing the vector.

Abstract: The invention provides Bean pod mottle virus (BPMV) vectors useful for expression of heterologous proteins in plants such as soybean. The BPMV vectors are also useful for virus-induced gene silencing. The vectors of the invention include modifications of BPMV RNA1 sequences so that infection with the vectors produces only moderate symptoms. The vectors also comprise novel RNA2 vectors which specifically provide for non-translated VIGS constructs and further which do not require in frame insertion of heterologous sequences to be expressed.

Abstract: This invention comprises a combined transgene/virus vector system for the expression of heterologous proteins in plants. While exemplified with respect to the bipartite RNA plant virus, Cowpect mosaic virus (CPMV), other bipartite viral systems may be used to advantage according to the method, system and composition described in detail herein.

Abstract: Disclosed herein are viral vectors suitable for transfection into woody trees for purposes of delivering and expressing beneficial genes. Specifically exemplified herein are vectors for transfecting citrus trees. The vectors allow for the expression of useful proteins, such as those that can protect the tree from disease. Specifically exemplified herein are methods of transfecting woody trees that allow multiple applications of vectors while avoiding superinfection exclusion.

Abstract: Nucleic acid comprising or encoding an RNA replica comprising, in this order, the following segments (i) to (iii): i) a nucleic acid sequence encoding a potexvirus RNA-dependent RNA polymerase or a function-conservative variant thereof; ii) a nucleic acid sequence comprising: a) a potexvirus triple gene block or a function-conservative variant thereof and b) a sequence encoding a potexviral coat protein or a function-conservative variant thereof; or a sequence encoding a tobago viral movement protein; and iii) a heterologous nucleic acid sequence expressible from said replica in a plant or in plant tissue.

Abstract: The present disclosure relates to the development and use of Closterovirus-based vectors for the delivery of nucleotides to plants. Specifically, the present disclosure provides viral vectors based on Grapevine leafroll-associated virus-2 for the delivery and expression of genes in plants, particularly grape plants. Methods of making and using these vectors, as well as the plants transformed by these vectors, are also contemplated.

Abstract: Disclosed herein are viral vectors suitable for transfection into woody trees for purposes of delivering and expressing beneficial genes. Specifically exemplified herein are vectors for transfecting citrus trees. The vectors allow for the expression of useful proteins, such as those that can protect the tree from disease. Specifically exemplified herein are methods of transfecting woody trees that allow multiple applications of vectors while avoiding superinfection exclusion.

Abstract: The present invention relates to a plant-infectious nucleic acid molecule from Pepper mottle virus, and a viral vector, a transformed cell and a transgenic plant having it. The present invention first achieves the cloning of the infectious full-length pepper mottle virus cDNA from virus-infected pepper, which enables to perform the molecular biological studies to the infectivity of pepper mottle virus in pepper and tobacco and to provide a plant virus-based vector to highly express a useful foreign protein.

Abstract: A series of viral vectors derived from foxtail mosaic virus, sunn hemp mosaic virus and white clover mosaic virus sequences, and which are capable of expressing heterologous proteins in plants, including the tobacco, Nicotiana benthamiana, and cowpea, bean, and other legumes with the properties of unusually limited expression in the absence of gene silencing suppressors and high level expression in their presence. In a preferred embodiment, these viral vectors may be introduced via agroinoculation, and expression of the heterologous gene may be inducible with little or no leakiness in the absence of induction.

Abstract: A method of controlling a genetically-modified plant or plant cells, said method comprising the following steps: (a) providing a genetically-modified plant or plant cells, said plant or plant cells containing a heterologous nucleic add encoding a first polypeptide containing or consisting of a first fragment of a protein, (b) introducing a second polypeptide into cells of said genetically-modified plant or plant cells, said second polypeptide containing (i) a second fragment of said protein and (ii) a peptide sequence enabling the introduction of said second polypeptide into cells of said genetically-modified plant or plant cells, whereby said first fragment and said second fragment jointly generate a predetermined function of said protein only when jointly present.

Abstract: A biological control agent for plants and a method of inoculating the plants with the biological control agent in order protect against plant disease are provided. The treatment method consists of inoculating the plant with a strain of cucumber mosaic virus (CMV), referred to as the KU1 strain, having an associated viral satellite RNA of SEQ ID NO: 1. Particularly, the KU1 strain may be used to protect plants against a particular viral strain of CMV, referred to as the KU2 strain, characterized by the associated viral satellite RNA of SEQ ID NO: 2, which causes tomato necrosis. Additionally, the KU1 strain may be used to protect against potato spindle tuber viroid (PSTV), which causes tomato stunting, fusarium wilt disease in the tomato (caused by Fusarium oxysporum f. sp. Lycopersicae), and leaf spotting disease in the tomato (caused by Alternaria alternate).

Abstract: The present invention provides trans-complementation systems for expressing gene products in plants. In general, the invention provides systems including a carrier vector and a producer vector, both based on plant viruses. The producer vector is defective for at least one function needed for successful systemic infection of a plant, e.g., replication, cell-to-cell movement, or long distance movement. The carrier vector supplies the missing function in trans. Certain producer vectors lack a functional coat protein coding sequence, in which case the corresponding producer vector supplies coat protein in trans. The invention also provides novel plant viral vectors and methods of use, e.g., to produce polypeptides or active RNAs in plants.

Abstract: A process of causing a targeted integration of DNA of interest into a plant cell nuclear genome, comprising; i) providing plant cells with an amplification vector, or a precursor thereof, capable of autonomous replication in plant cells, said vector comprising; a) DNA sequence(s) encoding an origin of replication functional in plant cells, b) DNA sequence(s) necessary for site-specific and/or homologous recombination between the vector and a host nuclear DNA, and c) optionally, further DNA of interest; ii) optionally providing conditions that facilitate vector amplification and/or cell to cell movement and/or site-specific and/or homologous recombination, and iii) selecting cells having undergone recombination at a predetermined site in the plant nuclear DNA.

Abstract: A method of controlling a genetically-modified plant, comprising (a) providing a genetically-modified plant, whereby cells of said genetically-modified plant contain a heterologous nucleic acid and whereby said genetically-modified plant is inactive with regard to a cellular process of interest, (b) switching on said cellular process of interest by directly introducing a polypeptide from a cell-free composition into cells containing said heterologous nucleic acid wherein said polypeptide and said heterologous nucleic acid are mutually adapted such that said polypeptide is capable of switching on said cellular process of interest.

Abstract: According to the invention, there is provided seed and plants of the corn variety designated CV112211. The invention thus relates to the plants, seeds and tissue cultures of the variety CV112211, and to methods for producing a corn plant produced by crossing a corn plant of variety CV112211 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety CV112211 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV112211.

Abstract: The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems isolated from or derived from non-bacterial organisms, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.

Abstract: The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems isolated from or derived from non-bacterial organisms, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.

Abstract: A method of generating genotypic variation in a genome of a plant is disclosed. The method comprising introducing into the plant at least one viral expression vector encoding at least one chimeric nuclease which comprises a DNA binding domain, a nuclease and a localization signal to a DNA-containing organelle, wherein the DNA binding domain mediates specific targeting of the nuclease to the genome of the plant, thereby generating genotypic variation in the genome of the plant.

Abstract: The present invention provides an improved process for the production of recombinant peptides. In particular, the present invention provides an improved process for the production of recombinant peptides in the form of viral capsid fusion proteins which can be assembled in vivo in plant cell suspension cultures. The invention also includes plasmids, sequences, and plant cells which allow for non-infectious viral capsid fusion peptide production.

Abstract: The invention concerns a method for obtaining potyvirus resistant plants exhibiting one or several mutations in a preserved region of the eIF4E translation factor, defined by the following general sequence (I): DX1X2X3X4KSX5QX6AWGSSX7RX8X9YTFSX10VEX11FWX12X13YNNIHX14P SKLX15X16GAD wherein: —X1, X2, X3, X4, X6, X7, X8, X9, X10, X12, X13, X15, et X16 represent each a neutral amino acid; X5 and X14 represent a basic amino acid; X11 represents an acid amino acid; D, K, S, Q, A, W, G, R, Y, T, F, V, E, N, I, H, P, and L have their usual one-letter code meaning.

Abstract: The invention described herein provides a C-terminally truncated interferon having a deletion of 7, 8, or 9 amino acids and having enhanced biological activity, and the polynucleotides encoding such an interferon. Also provided are methods for producing and using such truncated interferon. In some embodiments, the truncated interferon is produced in a plant.

Abstract: The present disclosure relates to the development and use of Closterovirus-based vectors for the delivery of nucleotides to plants. Specifically, the present disclosure provides viral vectors based on Grapevine leafroll-associated virus-2 for the delivery and expression of genes in plants, particularly grape plants. Methods of making and using these vectors, as well as the plants transformed by these vectors, are also contemplated.

Abstract: The invention relates to methods and compositions for identifying maize plants that have newly conferred resistance or enhanced resistance to, or are susceptible to, a Fijivirus, particularly Mal de Río Cuarto Virus (MRCV) and/or Maize Rough Dwarf Virus (MRDV). The methods use molecular genetic markers to identify, select and/or construct resistant plants or identify and counter-select susceptible plants. Maize plants that display newly conferred resistance or enhanced resistance to a Fijivirus that are generated by the methods of the invention are also a feature of the invention.

Abstract: According to the invention, there is provided seed and plants of the hybrid corn variety designated CH939349. The invention thus relates to the plants, seeds and tissue cultures of the variety CH939349, and to methods for producing a corn plant produced by crossing a corn plant of variety CH939349 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH939349.

Abstract: The invention relates to the soybean variety designated D5231451. Provided by the invention are the seeds, plants and derivatives of the soybean variety D5231451. Also provided by the invention are tissue cultures of the soybean variety D5231451 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety D5231451 with itself or another soybean variety and plants produced by such methods.

Abstract: A method of using a tospoviral nucleic acid molecule of the sequence of nt (nucleotide) 3975-4928 in accordance with GenBank Accession No. AF133128 or a full complement thereof comprising the steps of: (a) obtaining at least one fragment made from the tospoviral nucleic acid molecule; (b) obtaining a transgene from the at least one fragment; (c) introducing the transgene into a plant to generate a transgenic plant; (d) culturing the transgenic plant; (e) selecting a transgenic plant with broad-spectrum resistance; and (f) obtaining the transgenic plant with broad-spectrum resistance.

Abstract: The present invention provides systems and methods for producing pharmaceutically active proteins in transgenic sprouted seedlings. The transgenic sprouted seedlings of the invention are grown in a contained, regulatable environment, wherein expression of a pharmaceutically active protein is controlled by an exogenously inducible promoter. The transgenic sprouted seedlings may be eaten live or preferably harvested live to preserve the maximal biological activity of the pharmaceutically active protein.

Abstract: A novel transgenic corn event designated 3272, is disclosed. The invention relates to DNA sequences of the recombinant constructs inserted into the corn genome that resulted in the 3272 event and of genomic sequences flanking the insertion sites as well as to assays for detecting the presence of the 3272 event based on these novel sequences. The invention further relates to seeds of corn plants comprising the 3272 genotype, to corn plants comprising the genotype of 3272 and to methods for producing a corn plant by crossing a corn plant comprising the 3272 genotype with itself or another corn variety.

Abstract: A novel plant virus vector and a method of gene expression into plants are provided, and the present invention relates to a plant virus vector characterized in that a part of the sequence of the 2b region of the RNA2 molecule of the Cucumber mosaic virus (CMV) is deleted; the aforementioned plant virus vector characterized in that the region from the StuI site to the stop codon of the 2b ORF of pCY2, which is an infectious clone of CMV-Y RNA2, is deleted; and a method of gene expression in plants characterized in that a foreign gene is introduced so as to be stably expressed in a plant using the genetic recombinant. This vector is useful as a plant RNA vector for causing stable expression of foreign genes.

Abstract: The invention provides novel methods of gene targeting using replication in order to increase the efficiency of targeted genetic modification in an eukaryotic organism. Included are vectors, expression cassettes, and modified cells, plants and seeds.

Abstract: The invention discloses a method for breeding transgenic plants with the high antiviral property and the application of the method. The inventive method comprises the following steps of: a. checking the frequency of codon usage in a host and determining the rare codons in the host, modifying the codons in a target gene so that some codons in the target gene are mutated into the rare synonymous codons in the host plant; b. constructing a vector containing the target gene with the codon modifications, to be used for transforming plants; c. transforming the plants with the above recombinant vector to obtain the regenerative transgenic plants; d. detecting the transformed plants, screening the transgenic plants in which gene silencing occurs in the target gene, and thereby obtaining the transgenic plants with the high antiviral property. The invention method can be used in breeding highly efficient and stable antiviral transgenic plants.

Abstract: Provided are methods of increasing the tolerance of a plant to abiotic stresses and/or increasing the biomass and/or increasing the yield of a plant by expressing within the plant an exogenous polynucleotide homologous to SEQ ID NO:13.