Abstract: A fusion capsid protein comprising a plant virus capsid protein fused to an antigenic polypeptide is used as a molecule for presentation of that polypeptide to the immune system of an animal such as a human. The plant virus capsid protein is that of an alfalfa mosaic virus (AlMV) or ilarvirus.

Abstract: The present invention is directed to a DNA construct formed from a fusion gene which includes a trait DNA molecule and a silencer DNA molecule. The trait DNA molecule has a length that is insufficient to impart a desired trait to plants transformed with the trait DNA molecule. The silencer DNA molecule is operatively coupled to the trait DNA molecule with the trait and silencer DNA molecules collectively having sufficient length to impart the trait to plants transformed with the DNA construct. Expression systems, host cells, plants, and plant seeds containing the DNA construct are disclosed. The present invention is also directed to imparting multiple traits to a plant.

Abstract: A novel method of over expressing genes in plants is provided. This method is based on the RNA amplification properties of plus strand RNA viruses of plants. A chimeric multicistronic gene is constructed containing a plant promoter, viral replication origins, a viral movement protein gene, and one or more foreign genes under control of viral subgenomic promoters. Plants containing one or more of these recombinant RNA transcripts are inoculated with helper virus. In the presence of helper virus recombinant transcripts are replicated producing high levels of foreign gene RNA.

Abstract: Methods and mechanisms for regulation of macromolecular transport between cells in plasmodesmatal communication with one another are disclosed. Nuclear acids encoding tobacco mosaic virus movement protein (TMV-MP) in wild type and mutant forms is shown and used to affect plant size, carbon metabolism and biomass partitioning in transgeric plants.

Abstract: The present invention relates to a method for conferring herbicide, pest, and disease resistance in plant hosts. Specifically, the present invention employs transient viral expression vectors to express proteins or enzymes conferring resistance in plant hosts. In addition, a library of nucleotide sequence variants in a sense or antisene orientation may be used to determine the targets of an herbicide or pathogen and to screen suitable viral nucleic acids for herbicide, pest, and disease resistance.

Abstract: A gene amplification system based on plant viral genetic elements dramatically increases foreign protein production in plants. A safer and more economical production system for vaccines and antibodies in recombinant plants grown using agricultural practice is described. The high-level expression system uses the replicative process of a plant mastrevirus, exemplified by bean yellow dwarf virus (BeYDV). The expression system is preferably inducible to avoid interference with plant growth and development. Developmental cues, such as fruit ripening, are employed to trigger expression of the foreign protein using a tissue-specific promoter. A single, stably integrated expression cassette for foreign protein is replicated extrachromosomally in ripening fruit, forming hundreds of transcriptionally competent copies. Preferred plant hosts include tomato as a model system and soybean for production of large quantities of protein at high total protein levels.

Abstract: The present invention provides a chimeric recombinant DNA molecule comprising: a plurality of DNA sequences, each of which comprises a plant-functional promoter linked to a coding region, which encodes a virus-associated coat protein, wherein said DNA sequences are preferably linked in-tandem so that they are expressed in virus-susceptible plant cells transformed with said recombinant DNA molecule to impart resistance to said viruses; as well as methods for transforming plants with the chimeric constructs and for selecting plants which express at least one of said DNA sequences imparting viral resistance.

Abstract: The present invention relates to isolated Raspberry Bushy Dwarf Virus (RBDV) nucleic acid sequences which encode RBDV coat and movement proteins or polypeptides and mutant or modified forms thereof. The invention further relates to heterologous nucleic acid constructs, vectors, transformation methods, plant cells and plants comprising such RBDV-encoding nucleic acids and methods for inducing resistance to RBDV by transforming plants with a nucleic acid construct comprising RBDV protein or polypeptide-encoding nucleic acid sequences.

Abstract: Disclosed are nucleic acid vectors which comprise: (a) a transfer nucleotide sequence comprising (i) a plant active promoter, operably linked to (ii) a recombinant tobacco rattle virus (TRV) cDNA (preferably derived from TRV RNA2) which includes at least cis acting elements permitting replication of the cDNA; a subgenomic promoter operably linked to a sequence encoding a TRV coat protein; and a heterologous nucleotide sequence which is foreign to the virus;(b) border sequences which permit the transfer of the transfer nucleotide sequence into a plant genome. Such vectors may be used as expression vectors or for achieving viral induced gene silencing (VIGS) of a target gene, wherein the heterologous nucleotide sequence is a targeting sequence which corresponding to that gene. Example vectors include pTV00 and vectors which are derived from PTV00 and have the characteristics thereof. Also disclosed are associated processes, methods, viruses or viral particle, kits, host cells and plant tissues.

Abstract: CP genes of CMV strains V27, V33, V34, and A35 (CMV-V27, CMV-V33, CMV-V34, and CMV-A35 respectively) are provided.

Abstract: The present invention provides a chimeric recombinant DNA molecule comprising: a plurality of DNA sequences, each of which comprises a plant-functional promoter linked to a coding region, which encodes a virus-associated coat protein, wherein said DNA sequences are preferably linked in tandem so that they are expressed in virus-susceptible plant cells transformed with said recombinant DNA molecule to impart resistance to said viruses; as well as methods for transforming plants with the chimeric recombinant DNA molecule and for selecting plants which express at least one of said DNA sequences imparting viral resistance.

Abstract: The nucleotide sequences for tomato spotted wilt virus (TSWV) nucleocapsid is described, and transgenic plants containing the nucleocapsid nucicotide sequence from a TSWV isolate is shown to provide resistance in the transgenic plant to Tospoviruses from different serogroups. In addition, transgenic plants containing the nucleocapsid nucleotide sequence from a lettuce isolate of TSWV were produced and shown to provide (in plants producing small amounts of the nucleocapsid protein) resistance in the transgenic plant to both homologous and closely related viral isolates whereas plants producing larger amounts of the nucleocapsid protein possessed moderate levels of protection against both the homologous isolate and isolates of distantly related Impatiens necrotic spot virus (INSV).

Abstract: The invention relates to methods and compositions for site-specific recombinase-mediated mobilization of viral replicons from T-DNA. The methods comprise Agrobacterium-mediated transfer of T-DNA to a plant cell, wherein the T-DNA contains a viral replicon flanked by directly repeated target sites for a site-specific recombinase and optionally a DNA of interest linked to the viral replicon. The DNA of interest may also contain a non-identical target site for the recombinase. An expression cassette for the site-specific recombinase is present on the T-DNA or the plant genome, or is transiently introduced into the plant cell. Expression of the site-specific recombinase in the plant cell results in excision of the viral replicon and the associated DNA of interest. The viral replicon and DNA of interest are then replicated to high copy number in the host plant cell.

Abstract: The present invention concerns a method for inducing resistance to a virus comprising a TGB3 sequence with the proviso that it is not the potato virus X, into a plant cell or plant, comprising the following steps: preparing a nucleic acid construct comprising a nucleic acid sequence corresponding to at least 70% of the nucleic acid sequence of TGB3 of said virus or its corresponding cDNA, being operably linked to one or more regulatory sequence(s) active in a plant, transforming a plant cell with the nucleic acid construct, and possibly regenerating a transgenic plant from the transformed plant cell. The present invention is also related to the plant obtained.

Abstract: The invention involves production of transgenic plants containing DNA encoding AC1/C1 wildtype and mutant sequences that negatively interfere in trans with geminiviral replication during infection. The transgenic plants produced by the invention are resistant to viral infection.

Abstract: Disclosed are methods for producing transgenic grapevines with resistance to a plant pathogen, the method includes: transforming a plant cell of the genus Vitis with a nucleic acid which expresses a lytic peptide, where the expression of the lytic peptide provides resistance to a plant pathogen.

Abstract: The present invention is directed to transcription regulators and transcription regulator-like sequences of nanovirus origin. As used in the specification, the nanovirus group is consdiered to include subterranean clover stunt virus (SCSV), cocnut foliar decay virus (CFDV), banana bunchy top virus (BBTV), milk vetch dwarf cirus (MDV), and faba bean necrotic yellow virus (FBNYV). The transcription regulators and transcription regulator-like sequences of the instant invention are useful in genetic engineering of plants and in particular leguminous plants such as to facilitate or control expression of foreign genes. The transcription regulators and transcription regulator-like sequences of the present invention are also useful in facilitating different levels of expression in different plant tissue types.

Abstract: Transgenic plants transformed with a tomato aspermy virus 2b gene or active fragment thereof exhibit resistance to diseases caused by infectious pathogens, such as viruses. Expression of the 2b gene causes activation of hypersensitive response and expression of pathogenicity-related proteins in plants that are incapable of such a response to certain pathogens. Transformation of a wide variety of plants with expression vectors in which the tomato aspermy virus 2b gene is operably linked to a plant-active promoter renders the plant resistant to pathogenic infection.

Abstract: Procedure for the production of transgenic seedlings starting from genetically transformed buds, the said seedlings belonging to the species Cucumus melo and containing at least one gene introduced through the intermediary of Agrobacterium tumefaciens, characterized by the culture in two successive stages of genetically transformed buds, the first of these steps taking place in a plant cell culture medium containing a cytokinin and more particularly 6-benzyl aminopurine (BAP), and the second, which is performed when the buds have attained a height of about at least 3 mm, taking place in a plant cell culture medium containing as macro-elements: KH2PO4 about 50 to about 100 mgL−1 MgSO4 about 75 to about 300 mgL−1 CaCl2.2H2O about 500 to about 2500 mgL−1 KNO3 about 750 to about 1200 mgL−1 NH4NO3 about 150 to about 200 mgL−1.

Abstract: Nucleotide sequences produced by mutation (also known as mutant nucleotide sequences) of C1 nucleotide sequences present in a pathogenic geminivirus genome in plants with one or more mutations capable of producing a dominant negative phenotype for the replication of the pathogenic virus, its diffusion in a plant, or its spread from one plant to another, especially through vectors such as insects, the mutant nucleotide sequences being capable of fully or partially inhibiting the replication and/or diffusion and/or spread of the pathogenic virus for producing phytopathogenic geminivirus resistant or tolerant transgenic plants.

Abstract: Coat protein genes of cucumber mosaic virus strains V27, V33, V34 and A35 (CMV V27, CMV V33, CMV V34, and CMV A35 respectively) are provided.

Abstract: Impatiens is a major ornamental bedding and potted plant, and is an important component of the U.S. floral industry. Susceptibility to insect pests and diseases caused by pathogens remains a problem for Impatiens production, even under greenhouse conditions. While chemical treatment can control certain insect pests and disease pathogens, such treatment can also have an adverse effect upon Impatiens. The methods described herein provide a means to genetically engineer transgenic Impatiens that express macromolecules capable of protecting the plant against the insects and pathogens. The production of transgenic plants can also be used to enhance the commercial value of Impatiens by controlling or enhancing native Impatiens characteristics.

Abstract: A genetic construct comprising a trans-dominant inhibitor of geminiviral replication is disclosed. When expressed in a plant cell, this inhibitor is able to dramatically reduce replication of geminivirus. Genetic constructs that include sequences containing portion of the ac3 gene in addition to the trans-dominant inhibitor exhibit increased efficiency and broadened specificity of inhibition of geminiviral replication. A method of protecting plants against geminiviral infections is also disclosed.

Abstract: An isolated and purified DNA molecule comprising a sugarcane bacilliform virus promoter and expression cassettes comprising said promoter are provided. Also provided is a method of using a sugarcane bacilliform virus promoter to express proteins, RNA transcripts, or mixtures thereof, in transgenic plants.

Abstract: A method for producing genetically transgenic plants exhibiting resistance to a geminivirus such as tomato yellow leaf curl virus. In another aspect, the invention embraces chimeric plant genes, cassettes containing the chimeric plant genes, heterologous DNA segments containing the cassettes, and genetically transgenic cells and differentiated plants which exhibit resistance to geminiviruses. In yet another aspect, the invention embraces bacterial cells and plant transformation vectors comprising a heterologous DNA segment encoding for resistance to geminiviruses.

Abstract: Plant transformation vectors comprising a polynucleotide effective to render resisitance or tolerance to infection by a tospovirus, and a microbiological process for making virus tolerant or resistant plants are provided herein.

Abstract: The instant invention relates to the novel soybean cultivar designated CX392RR. Provided by the invention are the seeds, plants and derivatives of the soybean cultivar CX392RR. Also provided by the invention are tissue cultures of the soybean cultivar CX392RR and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean cultivar CX392RR with itself or another soybean variety, as well as the plants produced by such methods.

Abstract: A fusion capsid protein comprising a plant virus capsid protein fused to an antigenic polypeptide is used as a molecule for presentation of that polypeptide to the immune system of an animal such as a human. The plant virus capsid protein is that of an alfalfa mosaic virus (AlMV) or ilarvirus.

Abstract: The present invention provides methods and compositions for inhibiting virus infection in susceptible monocotyledonous plants. The methods and compositions involve the production of translationally altered forms of messenger RNA sequence derived from the inhibited virus. The invention further provides structural and organizational information for the genome of strain B of maize dwarf mosaic virus. Methods for inhibiting MDMV-B infection are taught. These methods include the generation of transformed plants containing chimeric genes capable of expressing either MDMV-B proteins or translationally altered forms of messenger RNA sequences produced by MDMV-B.

Abstract: The present invention relates to a novel method of inserting viral DNA, which optionally may contain cargo-DNA, into plants or viable parts thereof, but preferably into plants of the monocotyledon class, and most preferably into plants of the family Gramineae, using suitable transfer microorganisms. Further comprised by the invention are recombinant DNA, plasmid and vector molecules suitably adapted to the specific conditions of the process according to the invention and the transgenic plant products obtainable in accordance with the said process.

Abstract: A method for increasing virus resistance to a transgenic plant comprising a coat protein gene from a cucumber mosaic virus is provided.

Abstract: An isolated DNA sequence which codes for a luteo replicase gene is disclosed herein. A method for providing resistance to infection by a virus by expressing a replicase gene in plants is also disclosed, as are transgenic plants containing the replicase gene.

Abstract: NIb replicase gene of papaya ringspot virus replicase strain FLA.83 W is provided.

Abstract: The present invention relates to a coat protein gene of papaya ringspot virus strain FLA83 W. This coat protein gene can be used to prepare plants which are resistant to papaya ringspot virus.

Abstract: The invention provides a method for imparting resistance in animals to viruses, and in plants to viruses and viroids, that express double-stranded RNA-like structures (dsRNAs). This method enables the binding of pathogenic dsRNAs during the infection process by expression of dsRNA-binding protein in transgenic animal and plant hosts, thus interrupting the infection cycle and inhibiting disease. The presence of a dsRNA-binding protein in a transgenic host renders the transgenic host resistant to the phenotypic symptoms of viral infection and/or decreased pathogen replication. Accordingly, the present invention provides a genetically engineered animal and plant, stably transformed to express a dsRNA-binding protein, such that the transgenic host displays resistance to virus and/or viroid challenge.

Abstract: An isolated DNA sequence which codes for a potyvirus replicase gene is disclosed herein. A method for providing resistance to infection by a virus by expressing a replicase gene in plants is also disclosed. Transgenic potato plants and tubers containing the replicase gene are also disclosed.

Abstract: The present invention relates to novel, recombinant deoxyribonucleic acids (DNA), to vectors and host organisms which contain them, and to transgenic plants which contain the recombinant DNA and possess an elevated resistance toward pernicious organisms and plant diseases, where the recombinant deoxyribonucleic acids (DNA) are characterized in that they consist of a combination of the following components, or components possessing a DNA which in each case has the same effect, or contain these constituents:(a) a double-stranded cDNA fragment which is derived from the RNA of the plum pox virus (PPV) ("fragment A"), and(b) a double-stranded cDNA fragment which is derived from the S RNA of the tomato spotted wilt virus (TSWV) ("fragment B"), it being possible for these combinations also to possess, in addition to further DNA fragments which may optionally be present, a promoter which is active in plant cells.

Abstract: Provided are transgenic plants with resistance to viral infection polynucleotides and methods for conferring such resistance, and methods for producing the transgenic plants. Plants transformed with a nucleotide sequence encoding a P1 protein of potato virus Y result in plants having increased resistance to infection by potato virus Y.

Abstract: The present invention relates to a method of preparation of transgenic plants resistant to viral infections by introducing into the genoma of a host plant an antisense gene construct constituted by: the domain F of the subgenomic promoter of a viral RNA; a leader sequence of a viral ORF, downstream from said subgenomic promoter; the gene encoding a viral coat protein, downstream from said leader sequence; and the 3'-terminal region of a viral RNA, downstream from said gene. The present invention also relates to a recombinant vector comprising a promoter functional in a host plant, and, operably linked to this promoter, the antisense gene construct of the present invention.

Abstract: The present invention is directed to methods for the genetic transformation of pineapple plant tissue with Agrobacterium. The present invention also provides for the regeneration of intact pineapple plants from the transformed tissue.

Abstract: The present invention describes a method for the induction of resistance in a plant host to a RNA or DNA virus pathogenic to the plant which comprises isolating a fragment of viral RNA or DNA associated with the replicase portion of the virus genome, specifically a portion that does not involve a read-through portion of the gene, and integrating a DNA copy of the isolated fragment or a portion thereof into the genome of a recipient plant in such a manner that the plant becomes transformed with the inserted fragment.