The Polynucleotide Encodes An Inhibitory Rna Molecule Patents (Class 800/285)
  • Patent number: 10415050
    Abstract: The present invention relates to a mutant, non-naturally occurring or transgenic tobacco plant cell comprising: (i) a polynucleotide comprising, consisting or consisting essentially of a sequence encoding a functional nicotine N-demethylase and having at least 95% sequence identity to SEQ ID NO:2; (ii) a polypeptide encoded by the polynucleotide set forth in (i); (iii) a polypeptide comprising, consisting or consisting essentially of a sequence encoding a nicotine N-demethylase and having at least 95% sequence identity to SEQ ID NO:3; or (iv) a construct, vector or expression vector comprising the isolated polynucleotide set forth in (i), and wherein the expression or activity of said nicotine demethylase is reduced as compared to a control tobacco plant cell in which the expression or activity of said nicotine demethylase has not been reduced.
    Type: Grant
    Filed: May 7, 2015
    Date of Patent: September 17, 2019
    Assignee: Philip Morris Products S.A.
    Inventors: Verena Liedschulte, Simon Goepfert, Lucien Bovet, Nicolas Sierro
  • Patent number: 10233462
    Abstract: The present invention discloses gene targets, constructs and methods for the genetic control of plant disease caused by nematodes of the genus Meloidogyne (root knot nematodes). The present invention relates to achieving a plant protective effect through the identification of target coding sequences and the use of recombinant DNA technologies for post-transcriptionally repressing or inhibiting expression of the target coding sequences in the cells of plant-parasitic nematodes. The disclosed gene targets show significant conservation at the nucleotide level between orthologs from different Meloidogyne species, facilitating genus-wide targeting by RNA interference.
    Type: Grant
    Filed: March 7, 2017
    Date of Patent: March 19, 2019
    Assignee: Monsanto Technology LLC
    Inventors: John D. Bradley, Brandi J. Chiapelli, Bingli Gao, James P. McCarter, Michelle L. Gasper, Deryck Jeremy Williams
  • Patent number: 10072270
    Abstract: The present invention provides nucleic acids encoding transcription factors and methods of using these nucleic acids to modulate nicotine production in plants and to produce plants having modulated nicotine production.
    Type: Grant
    Filed: January 23, 2017
    Date of Patent: September 11, 2018
    Assignee: NORTH CAROLINA STATE UNIVERSITY
    Inventors: Rongda Qu, Bingwu Wang
  • Patent number: 9896698
    Abstract: The present invention presents methods to alter the genetic composition of crop plants susceptible to nematode infection to improve tolerance to the same. Methods and compositions for modulating key pathways involved in the syncytial event of nematode infection and for preventing the cascade of differential gene expression caused by the same as disclosed. Applicants have found that the microRNA miR396 acts as a master switch of syncytial gene expression changes in plants after infection, and further that miR396 and certain growth regulating transcription factors (GRF) are connected through feedback interaction in syncytium initiation and maintenance.
    Type: Grant
    Filed: July 8, 2015
    Date of Patent: February 20, 2018
    Assignee: Iowa State University Research Foundation, Inc.
    Inventors: Thomas J. Baum, Tarek Hewezi
  • Patent number: 9777313
    Abstract: The present invention provides a silver nanocluster probe which comprises a silver nanoparticle binding region and a specific nucleotide sequence region that specifically binds to a target polynucleotide, wherein the silver nanocluster probe is configured such that it will emit detectable light when silver nanoparticles bind to the silver nanoparticle binding region to form a silver nanocluster, but light emission from the silver nanocluster probe will decrease or decay when the target polynucleotide binds to the specific nucleotide sequence region. According to the present invention, either the presence of a target polynucleotide in a sample or a mutation in the target polynucleotide can be detected in a rapid and convenient manner by determining whether light emission decreases or decays when the target polynucleotide binds to the specific nucleotide sequence region of the silver nanocluster probe that emits detectable light.
    Type: Grant
    Filed: August 22, 2012
    Date of Patent: October 3, 2017
    Assignee: SEOULIN BIOSCIENCE CO., LTD.
    Inventors: Seong Wook Yang, Tom Vosch
  • Patent number: 9777288
    Abstract: Disclosed herein are methods of controlling insect pests, in particular Leptinotarsa spp. which infest crop plants, and methods of providing plants resistant to such pests. Also disclosed are polynucleotides and recombinant DNA molecules and constructs useful in such methods, insecticidal compositions such as topical sprays containing insecticidal double-stranded RNAs, and solanaceous plants with improved resistance to infestation by Leptinotarsa spp. Further disclosed are methods of selecting target genes for RNAi-mediated silencing and control of Leptinotarsa spp.
    Type: Grant
    Filed: July 18, 2014
    Date of Patent: October 3, 2017
    Assignee: MONSANTO TECHNOLOGY LLC
    Inventors: Jodi Lynn Beattie, Michael John Crawford, Brian Donovan Eads, Lex Evan Flagel, Mahak Kapoor, Christina Marie Taylor
  • Patent number: 9708619
    Abstract: The present invention is in the field of genetics, especially plant genetics, and provides agents capable of controlling gene expression. More specifically the inventions relates to methods for engineering ta-siRNA primary transcripts in order to target gene-of-interest (GOI) and control their expression. The invention further provides for a method for modulating transgenic expression by said engineered ta-siRNAs.
    Type: Grant
    Filed: September 20, 2006
    Date of Patent: July 18, 2017
    Assignee: BASF Plant Science GmbH
    Inventors: Peifeng Ren, Hee-Sook Song, Yuwen Wang, John McMillan
  • Patent number: 9701974
    Abstract: Methods of, and compositions for, assembling one or more transcription units in a genome without a linked selectable marker or other unwanted transcription unit are provided. Also provided methods of, and compositions for, assembling one or more transcription units in a genome with a reduced frequency of vector backbone.
    Type: Grant
    Filed: October 22, 2014
    Date of Patent: July 11, 2017
    Assignee: Monsanto Technology LLC
    Inventors: Larry A. Gilbertson, Susan J. Johnson
  • Patent number: 9657293
    Abstract: Disclosed are double stranded RNA molecules that are toxic to coleopteran insects. In particular, dsRNA molecules that capable of interfering with pest IAP genes and that are toxic to the target pest are provided. Further, methods of making and using the interfering RNA, for example in transgenic plants to confer protection from insect damage are disclosed.
    Type: Grant
    Filed: June 19, 2013
    Date of Patent: May 23, 2017
    Assignee: Sygenta Participations AG
    Inventors: Kevin Donohue, Renshui Liu, Jeng Shong Chen
  • Patent number: 9580722
    Abstract: The present invention provides nucleic acids encoding transcription factors and methods of using these nucleic acids to modulate nicotine production in plants and to produce plants having modulated nicotine production.
    Type: Grant
    Filed: February 27, 2014
    Date of Patent: February 28, 2017
    Assignee: North Carolina State University
    Inventors: Rongda Qu, Bingwu Wang
  • Patent number: 9523089
    Abstract: The present invention relates to nucleic acids and nucleic acid fragments encoding amino acid sequences for flavonoid biosynthetic enzymes in plants, and the use thereof for the modification of flavonoid biosynthesis in plants. More particularly, the flavonoid biosynthetic enzyme is selected from the group consisting of chalcone isomerase (CHI), chalcone synthase (CHS), chalcone reductase (CHR), dihydroflavonol 4-reductase (DFR), leucoanthocyanidin reductase (LCR), flavonoid 3?,5? hydrolase (F3?5?H), flavanone 3-hydroxylase (F3H), flavonoid 3?-hydroxylase (F3?H), phenylalanine ammonia-olyase (PAL) and vestitone reductase (VR), and functionally active fragments and variants thereof.
    Type: Grant
    Filed: September 13, 2013
    Date of Patent: December 20, 2016
    Assignees: Agriculture Victoria Services Pty LTD, AgResearch Limited
    Inventors: German Spangenberg, Timothy Ivor Sawbridge, Eng-Kok Ong, Michael Emmerling
  • Patent number: 9416396
    Abstract: A biological process indicator is provided for validating a treatment process in which the amount or activity of a contaminant in a sample is reduced. The indicator comprises a thermostable kinase covalently linked to a biological component, with the proviso that the biological component is not an antibody. Methods of preparing the indicator, and methods of using the indicator, are also provided.
    Type: Grant
    Filed: February 18, 2009
    Date of Patent: August 16, 2016
    Assignee: The Secretary of State for Health
    Inventors: J. Mark Sutton, J. Richard Hesp, Michael Ungurs
  • Patent number: 9388409
    Abstract: The invention relates to identifying and evaluating target coding sequences for control of plant parasitic nematodes by inhibiting one or more biological functions, and their use. The invention provides methods and compositions for identification of such sequences and for the control of a plant-parasitic nematode population. By feeding one or more recombinant double stranded RNA molecules provided by the invention to the nematode, a reduction in disease may be obtained through suppression of nematode gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and the plant cells and plants obtained thereby.
    Type: Grant
    Filed: August 27, 2013
    Date of Patent: July 12, 2016
    Assignee: Monsanto Technology LLC
    Inventors: Andrey A. Boukharov, Zijin Du, Liang Guo, Michelle C. Hresko, David K. Kovalic, Zhaolong Li, Maolong Lu, James P. McCarter, Nancy M. Miller, Mark Vaudin, Deryck J. Williams, Wei Wu
  • Patent number: 9322030
    Abstract: The present invention features tobacco nicotine demethylase nucleic acid and amino acid sequences, tobacco plants and plant components containing such sequences, including tobacco plants and plant components having reduced expression or altered enzymatic activity of nicotine demethylase, methods of use of nicotine demethylase sequences to create plants having altered levels of nornicotine or N?-nitrosonornicotine (“NNN”) or both relative to a control plant, as well as tobacco articles having reduced levels of nornicotine or NNN.
    Type: Grant
    Filed: November 22, 2013
    Date of Patent: April 26, 2016
    Assignee: U.S. Smokeless Tobacco Company LLC
    Inventor: Dongmei Xu
  • Patent number: 9309512
    Abstract: This invention discloses recombinant DNA constructs encoding phased small RNAs useful in regulating expression of one or more genes of interest. Also disclosed by this invention are transgenic plant cells, plants, and seeds containing a recombinant DNA construct of this invention.
    Type: Grant
    Filed: February 25, 2013
    Date of Patent: April 12, 2016
    Assignee: Monsanto Technology LLC
    Inventors: Edwards Allen, Liang Guo, Sara E. Heisel, Sergey I. Ivashuta, Yuanji Zhang
  • Patent number: 9212370
    Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
    Type: Grant
    Filed: March 18, 2010
    Date of Patent: December 15, 2015
    Assignee: Monsanto Technology LLC
    Inventors: Edwards M. Allen, Larry A. Gilbertson, Sara E. Heisel, Shihshieh Huang, Elysia K. Krieger, Thomas M. Malvar
  • Patent number: 9145562
    Abstract: A variegated plant comprising a nucleic acid operatively linked to a regulatory region, the nucleic acid disrupts the expression of Cpn21. The nucleic acid is typically an antisense Cpn21 and the regulatory region may be an inducible regulatory region, a tissue specific regulatory region, or a developmental regulatory region.
    Type: Grant
    Filed: November 20, 2009
    Date of Patent: September 29, 2015
    Assignee: ALBERTA INNOVATES—TECHNOLOGY FUTURES
    Inventors: Margarita Velcheva, John Vidmar, Jurgen Quandt
  • Patent number: 9057049
    Abstract: The aromatic compound 2-acetyl-1-pyrroline-is the major potent flavor component of all aromatic rice and other plants. This present invention provides transgenic plants in which 2-acetyl-1-pyrroline is synthesized at a level greater than in naturally occurring non-aromatic varieties. The transgenic plants have reduced expression of the Os2AP gene and protein, resulting in an aromatic phenotype.
    Type: Grant
    Filed: December 6, 2010
    Date of Patent: June 16, 2015
    Assignees: Kasetsart University, National Science and Technology Development Agency
    Inventors: Apichart Vanavichit, Somvong Tragoonrung, Theerayut Toojinda, Samart Wanchana, Wintai Kamolsukyunyong, Siwaret Arikit
  • Patent number: 9044019
    Abstract: Compositions and methods for modulating flowering, sugar metabolism and stress response in plants are provided.
    Type: Grant
    Filed: May 24, 2011
    Date of Patent: June 2, 2015
    Assignee: RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY
    Inventors: Joachim Messing, Martin Calviño, Rémy Bruggmann
  • Patent number: 9040775
    Abstract: The present invention relates to methodology and constructs for modifying plant architecture and enhancing plant biomass and/or sucrose yield.
    Type: Grant
    Filed: March 8, 2012
    Date of Patent: May 26, 2015
    Assignee: Monsanto Do Brasil LTDA.
    Inventors: Paula Gonçalves De Araujo, Jesus Aparecido Ferro, Marcos Alegria, Ricardo Augusto Dante
  • Patent number: 9040774
    Abstract: This invention provides recombinant DNA constructs and methods for manipulating expression of a target gene that is regulated by a small RNA, by interfering with the binding of the small RNA to its target gene. More specifically, this invention discloses recombinant DNA constructs encoding cleavage blockers, 5-modified cleavage blockers, and translational inhibitors useful for modulating expression of a target gene and methods for their use. Further disclosed are miRNA targets useful for designing recombinant DNA constructs including miRNA-unresponsive transgenes, miRNA decoys, cleavage blockers, 5-modified cleavage blockers, and translational inhibitors, as well as methods for their use, and transgenic eukaryotic cells and organisms containing such constructs.
    Type: Grant
    Filed: July 1, 2009
    Date of Patent: May 26, 2015
    Assignee: Monsanto Technology LLC
    Inventors: Sergey I. Ivashuta, Barbara E. Wiggins, Yuanji Zhang
  • Publication number: 20150135364
    Abstract: The invention pertains to a method for generating a heat-resistant plant, including the step of introducing a polynucleotide, having a first sequence portion for transcriptionally driving a second sequence portion that is operably linked to a second sequence portion encoding for a miRNA156 family member into a plant cell, wherein the first sequence portion is heat-stress inducible.
    Type: Application
    Filed: November 6, 2014
    Publication date: May 14, 2015
    Inventor: Isabel Bäurle
  • Patent number: 9029636
    Abstract: This disclosure provides purified nucleic acids and polypeptides. Also provided are transgenic plants, seeds, and plant cells containing DNA for expression of the proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants, methods of making such plants and methods of making agricultural commodity including seeds and hybrid seeds from such plants.
    Type: Grant
    Filed: February 2, 2009
    Date of Patent: May 12, 2015
    Assignee: Monsanto Technology LLC
    Inventors: Wei Wu, Jack Tabaska, David Kovalic, Bo-Xing Qiu, Liang Guo
  • Publication number: 20150121570
    Abstract: The present invention relates to a composition for improving a tolerance to drought and oxidative stress of a plant, a plant transformed with the composition, a method for preparing a plant transformed with the composition, a method for improving a tolerance to a drought and oxidative stress and a composition and method for a germination rate of a plant. The nucleotide sequences of the present invention are involved in the drought and oxidative stress-tolerance and germination capacity, therefore may be effectively used for cultivating the plants with enhanced tolerance against abiotic stress or novel function of rapid growing or bolting.
    Type: Application
    Filed: April 22, 2014
    Publication date: April 30, 2015
    Applicant: Industry-Academic Cooperation Foundation, Yonsei University
    Inventors: Woo Taek KIM, Eun Yu KIM, Moon Yong RYU, Yoon A CHOI
  • Publication number: 20150121569
    Abstract: Process for detecting and identifying micropeptides (miPEPs) encoded by a nucleotide sequence contained in the sequence of the primary transcript of a microRNA and use thereof for modulating gene expression.
    Type: Application
    Filed: July 1, 2014
    Publication date: April 30, 2015
    Applicants: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, UNITERSITE PAUL SABATIER TOULOUSE III
    Inventors: Jean-Philippe COMBIER, Dominique LAURESSERGUES, Guillaume BECARD, Francois PAYRE, Serge PLAZA, Jerome CAVAILLE, Jerome CAVAILLE
  • Publication number: 20150121571
    Abstract: The present invention relates to a composition for improving a tolerance to a salt stress or a drought stress of a plant, and a method for preparing transformed plant exhibiting improved tolerance to salt stress or drought stress. The nucleotide sequences of the present invention are involved in salt or drought stress-tolerance, therefore may be effectively used for cultivating the plants with novel functional features which are less affected by climates and environments of the cultivated areas.
    Type: Application
    Filed: July 10, 2014
    Publication date: April 30, 2015
    Inventors: Woo Taek KIM, Jae Hwan HWANG, Dong Hye SEO
  • Patent number: 9018448
    Abstract: The present invention pertains to a novel and extensive analytical platform for selecting genes with a possible commercial phenotype from a large group of candidate genes identified using tools in bioinformatics, data from EST sequencing and DNA array. An aspect of the invention provides a method of producing a transgenic plant having an increased growth compared to its wild type. The method comprises altering in the plant the level of a gene product of at least one gene specifically expressed during different phases of wood formation. Further aspects of the invention provide a plant cell or plant progeny of a transgenic plant comprising a recombinant polynucleotide according to the invention. Other aspects pertain a DNA construct comprising a nucleotide sequence of the invention and a plant cell or plant progeny comprising the DNA construct.
    Type: Grant
    Filed: December 4, 2007
    Date of Patent: April 28, 2015
    Assignee: SweTree Technologies AB
    Inventors: Magnus Hertzberg, Göran Sandberg, Jarmo Schrader, Carl David Jonsén
  • Patent number: 9018002
    Abstract: A method to generate siRNAs in vivo is described, as are constructs and compositions useful in the method. The method does not depend on the use of DNA or synthetic constructs that contain inverted duplications or dual promoters so as to form perfect or largely double-stranded RNA. Rather, the method depends on constructs that yield single-stranded RNA transcripts, and exploits endogenous or in vivo-produced miRNAs or siRNAs to initiate production of siRNAs. The miRNAs or siRNAs guide cleavage of the transcript and set the register for production of siRNAs (usually 21 nucleotides in length) encoded adjacent to the initiation cleavage site within the construct. The method results in specific formation of siRNAs of predictable size and register (phase) relative to the initiation cleavage site. The method can be used to produce specific siRNAs in vivo for inactivation or suppression of one or more target genes or other entities, such as pathogens.
    Type: Grant
    Filed: March 14, 2013
    Date of Patent: April 28, 2015
    Assignee: State of Oregon acting by and through the State Board of Higher Education on behalf of Oregon State University
    Inventors: James C. Carrington, Edwards Allen
  • Patent number: 9012722
    Abstract: This disclosure concerns nucleic acid molecules and methods of use thereof for control of coleopteran pests through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in coleopteran pests. The disclosure also concerns methods for making transgenic plants that express nucleic acid molecules useful for the control of coleopteran pests, and the plant cells and plants obtained thereby.
    Type: Grant
    Filed: December 30, 2011
    Date of Patent: April 21, 2015
    Assignee: Dow AgroSciences LLC
    Inventors: Kenneth Narva, Huarong Li, Chaoxian Geng, Ignacio M. Larrinua, Monica B. Olson, Navin Elango
  • Patent number: 9012723
    Abstract: This disclosure provides purified nucleic acids and polypeptides. Also provided are transgenic plants, seeds, and plant cells containing DNA for expression of the proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants, methods of making such plants and methods of making agricultural commodity including seeds and hybrid seeds from such plants.
    Type: Grant
    Filed: January 13, 2010
    Date of Patent: April 21, 2015
    Assignee: Monsanto Technology LLC
    Inventors: Liang Guo, David K. Kovalic, Bo-Xing Qiu, Jack E. Tabaska, Wei Wu
  • Patent number: 9000264
    Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
    Type: Grant
    Filed: July 8, 2010
    Date of Patent: April 7, 2015
    Assignee: Monsanto Technology LLC
    Inventors: Edwards M. Allen, Larry A. Gilbertson, Nancy M. Houmard, Shihshieh Huang, Elysia K. Krieger, Thomas M. Malvar
  • Publication number: 20150096078
    Abstract: Disclosed herein are viral vectors based on modifications of the Citrus Tristeza virus useful for transfecting citrus trees for beneficial purposes. Included in the disclosure are viral vectors including one or more gene cassettes that encode heterologous polypeptide s. The gene cassettes are positioned at desirable locations on the viral genome so as to enable expression while preserving functionality of the virus. Also disclosed are methods of transfecting plants and plants transfected with viral vector embodiments.
    Type: Application
    Filed: May 5, 2014
    Publication date: April 2, 2015
    Inventors: William O. Dawson, Svetlana Folimonova, Choaa Amine El Mohtar, Siddarame Gowda, Subhas Hajeri
  • Patent number: 8993842
    Abstract: Methods and means for producing xylan structures in plants having a non-native saccharide moiety substitution side chain component, plants and plant cells comprising modified xylan structures, methods of identifying mutant plants comprising xylan structures in plants having a non-native saccharide moiety substitution pattern side chain component, uses thereof, and isolated xylan structures and uses thereof.
    Type: Grant
    Filed: September 17, 2008
    Date of Patent: March 31, 2015
    Assignee: Cambridge Enterprise Limited
    Inventors: Paul Dupree, Godfrey Preston Miles
  • Patent number: 8987552
    Abstract: Nucleic acid molecules and methods of use thereof for control of coleopteran pests through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in coleopteran pests are disclosed. Methods for making transgenic plants that express nucleic acid molecules useful for the control of coleopteran pests, and the plant cells and plants obtained thereby are also disclosed.
    Type: Grant
    Filed: December 30, 2011
    Date of Patent: March 24, 2015
    Assignee: Dow AgroSciences, LLC.
    Inventors: Kenneth Narva, Huarong Li, Chaoxian Geng, Ignacio M. Larrinua, Monica B. Olson, Navin Elango
  • Patent number: 8987554
    Abstract: A method is disclosed for reducing the level of gossypol in cottonseed. The method generally includes selectively inducing RNA gene silencing in the seed of a transgenic cotton plant, to interfere with expression of the ?-cadinene synthase gene or the ?-cadinene-8-hydroxylase gene in the seed of the cotton plant without substantially affecting expression of that gene in the foliage, floral parts, and roots of the plant. The transgenic cotton plant comprises at least one of a ?-cadinene synthase gene trigger sequence and/or a ?-cadinene-8-hydroxylase gene trigger sequence operably linked to one or more a seed-specific promoter gene sequences, and the trigger sequence(s) is/are able to induce RNA gene silencing when expressed in cottonseed of the plant.
    Type: Grant
    Filed: May 17, 2011
    Date of Patent: March 24, 2015
    Assignee: The Texas A&M University System
    Inventors: Keerti S. Rathore, Ganesan Sunilkumar, LeAnne M. Campbell
  • Patent number: 8987556
    Abstract: The present invention provides isolated polynucleotide sequences encoding ?-D-N-acetylhexosaminidase. The present invention further provides DNA construct comprising the polynucleotide sequence coding for ?-D-N-acetylhexosaminidase in sense or antisense orientation, RNAi construct, recombinant vector comprising the construct and host cells comprising the recombinant vector disclosed in the present invention. The present invention further provides transgenic plant, plant cell, transgenic progeny and seeds expressing the polynucleotide with reduced ?-D-N-acetylhexosaminidase protein accumulation, having enhanced fruit shelf life.
    Type: Grant
    Filed: July 9, 2009
    Date of Patent: March 24, 2015
    Assignee: National Institute of Plant Genome Research
    Inventors: Asis Datta, Subhra Chakraborty, Niranjan Chakraborty, Vijaykumar Meli Siddesh, Sumit Ghosh
  • Patent number: 8987553
    Abstract: The invention provides methods for improving plant yield, particularly under nitrogen limiting conditions. According to the invention, applicants have discovered that modulating ACC synthase activity in plants improves yield of plants, even when grown under low nitrogen conditions. The same plants, while demonstrating improved yield over non-modified plants, exhibited no deleterious effects under normal nitrogen conditions. The invention further provides methods using recombinant expression cassettes, host cells and transgenic plants.
    Type: Grant
    Filed: April 14, 2010
    Date of Patent: March 24, 2015
    Assignee: Pioneer Hi Bred International Inc
    Inventors: Nicholas J. Bate, Sarah T. Collinson, Jeffrey E. Habben, Honor Renee Lafitte, Kellie Reimann
  • Patent number: 8987555
    Abstract: Two genes, A622 and NBB1, can be influenced to achieve a decrease of nicotinic alkaloid levels in plants. In particular, suppression of one or both of A622 and NBB1 may be used to decrease nicotine in tobacco plants.
    Type: Grant
    Filed: July 23, 2014
    Date of Patent: March 24, 2015
    Assignee: 22nd Century Limited, LLC
    Inventors: Takashi Hashimoto, Akira Kato
  • Publication number: 20150082490
    Abstract: The present invention relates to the field of RNA-mediated gene silencing in insect species. The present invention is based, in part, on the inventors' sequencing of genes from eucalyptus invasive species Gb pest, Glycaspis brimblecombei. In certain aspects, the invention provides Gb nucleic acids, derivatives thereof and the use of such nucleic acids and derivatives as Gb control agents.
    Type: Application
    Filed: April 22, 2013
    Publication date: March 19, 2015
    Inventors: Dror Avisar, Daniel Siegel, Ziv Shani
  • Patent number: 8981181
    Abstract: Methods and compositions useful in target sequence suppression and target sequence validation are described. Polynucleotide constructs useful for gene silencing, as well as cells, plants and seeds comprising the polynucleotides and a method for using microRNAs to silence a target sequence are also described.
    Type: Grant
    Filed: December 8, 2010
    Date of Patent: March 17, 2015
    Assignee: E. I. du Pont de Nemours and Company
    Inventors: Milo Aukerman, Hajime Sakai, James Tisdall, Jeanne M. Wilson
  • Patent number: 8975470
    Abstract: The present invention provides means and methods for simple and efficient introduction of foreign genetic material into the plant cell. Particularly, the present invention combines seed priming and virus-based DNA constructs for efficient introduction of heterologous DNA into plants.
    Type: Grant
    Filed: June 30, 2010
    Date of Patent: March 10, 2015
    Assignee: Yissum Research Development Company of the Hebrew University of Jerusalem Ltd.
    Inventors: Ilan Sela, Haim David Rabinowitch, Ofer Gover
  • Patent number: 8975471
    Abstract: The invention provides methods and compositions useful in target sequence suppression, target sequence validation and target sequence down regulation. The invention provides polynucleotide constructs useful for gene silencing or RNA down regulation, as well as cells, plants and seeds comprising the polynucleotides. The invention also provides a method for using microRNA to silence a target sequence or to down regulate RNA.
    Type: Grant
    Filed: October 12, 2005
    Date of Patent: March 10, 2015
    Assignee: The Rockefeller University
    Inventors: Jose L. Reyes Taboada, Xiuren Zhang, Takashi Soyano, Nam-Hai Chua, Qi-Wen Niu, Shih-Shun Lin
  • Publication number: 20150067913
    Abstract: Pairs of plants are provided in which complementing constructs result in suppression of a parental phenotype in the progeny. Methods to generate and maintain such plants and methods of use of said plants, are provided, including use of parental plants to produce sterile plants for hybrid seed production. Also provided are methods for maintaining a homozygous recessive condition and for repressing transmission of transgenes.
    Type: Application
    Filed: November 17, 2014
    Publication date: March 5, 2015
    Inventors: Tim Fox, Howard P Hershey, Yongzhong Wu, Andrew Cigan, Erica Unger-Wallace
  • Patent number: 8962918
    Abstract: The present invention provides isolated polynucleotide sequences encoding ?-mannosidase. The present invention further provides DNA constructs comprising the polynucleotide sequence coding for ?-mannosidase in sense or anti-sense orientation, RNAi constructs, recombinant vectors comprising the constructs, and host cells comprising the recombinant vector. The present invention further provides transgenic plants, plant cells, transgenic progeny and seeds expressing the polynucleotide with reduced ?-mannosidase protein accumulation, having enhanced fruit shelf life.
    Type: Grant
    Filed: July 9, 2009
    Date of Patent: February 24, 2015
    Assignee: National Institute of Plant Genome Research
    Inventors: Asis Datta, Subhra Chakraborty, Niranjan Chakraborty, Sumit Ghosh, Vijaykumar Siddesh Meli
  • Publication number: 20150047066
    Abstract: By a QTL analysis and so forth using 4-HPPD inhibitor-susceptible rice and 4-HPPD inhibitor-resistant rice, a hypothetical gene (HIS1 gene) of an iron/ascorbate-dependent oxidoreductase gene located on a short arm of chromosome 2 of rice has been identified as a 4-HPPD inhibitor-resistance gene. Further, it has also been revealed that a homologous gene (HSL1 gene) of the HIS1 gene is located on chromosome 6 of rice. Furthermore, it has been found out that utilizations of these genes make it possible to efficiently produce a plant having increased resistance or susceptibility to a 4-HPPD inhibitor and to efficiently determine whether a plant has resistance or susceptibility to a 4-HPPD inhibitor.
    Type: Application
    Filed: December 26, 2011
    Publication date: February 12, 2015
    Applicants: TOYAMA PREFECTURE, SDS BIOTECH K.K., Incorporated Adminstrative Agency National agriculture and Food Research Organization
    Inventors: Hiroshi Kato, Hideo Maeda, Yoshihiro Sunohara, Ikuo Ando, Masahiro Oshima, Motoshige Kawata, Hitoshi Yoshida, Sakiko Hirose, Makiko Kawagishi, Yojiro Taniguchi, Kazumasa Murata, Hiroaki Maeda, Yuji Yamada, Keisuke Sekino, Akihiko Yamazaki
  • Patent number: 8946511
    Abstract: This invention discloses novel microRNAs and their precursors, and recombinant DNA constructs including such novel miRNAs, miRNA precursors, miRNA promoters, and miRNA recognition sites corresponding to the miRNAs. Included are novel miRNA and miRNA precursors that exhibit nutrient-responsive expression. Also disclosed are miRNA decoy sequences. Further provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention and methods of controlling gene expression using recombinant DNA constructs of this invention.
    Type: Grant
    Filed: October 12, 2007
    Date of Patent: February 3, 2015
    Assignee: Monsanto Technology LLC
    Inventors: Edwards Allen, Barry S. Goldman, Liang Guo, Sara E. Heisel, Shihshieh Huang, Sergey I. Ivashuta, David K. Kovalik, Elysia K. Krieger, James K. Roberts, Yuanji Zhang
  • Patent number: 8946510
    Abstract: The present invention is directed to controlling pest infestation by inhibiting one or more biological functions in an invertebrate pest. The invention discloses methods and compositions for use in controlling pest infestation by feeding one or more different recombinant double stranded RNA molecules to the pest in order to achieve a reduction in pest infestation through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.
    Type: Grant
    Filed: April 8, 2005
    Date of Patent: February 3, 2015
    Assignee: Monsanto Technology LLC
    Inventors: James A. Baum, Larry A. Gilbertson, David K. Kovalic, Thomas J. LaRosa, Maolong Lu, Tichafa R. I. Munyikwa, James K. Roberts, Wei Wu, Bei Zhang
  • Patent number: 8937217
    Abstract: Isolated nucleic acid fragments comprising precursor miRNAs, and artificial miRNAs and their use in down-regulating gene expression are described.
    Type: Grant
    Filed: December 16, 2008
    Date of Patent: January 20, 2015
    Assignee: E. I. du Pont de Nemours and Company
    Inventor: Brian McGonigle
  • Publication number: 20150020237
    Abstract: The present invention relates to a method for preparing fertility-lowered plant. The present invention provides an RNA interference vector and a method for obtaining a transgenic plant by introducing said RNA interference vector into a target plant; said transgenic plant is the following 1) or 2): 1) Sterile transgenic plant or 2) The fertility of said transgenic plant is lower than said target plant; the present invention also provides a method for cultivating a target plant to a sterile mutant or fertility-lowered mutant by sodium azide mutagenesis. The experiments of the present invention proved that the present invention provides various methods for preparing sterile lines or fertility-lowered lines; including RNA interference or TILLING (Targeting Induced Local Lesions IN Genomes) technology selection; sterile lines prepared by the methods of the present invention establish the basis of rice heterosis and crossbreeding.
    Type: Application
    Filed: November 14, 2012
    Publication date: January 15, 2015
    Applicant: Institute of Botany of the Chinese Academy of Sciences
    Inventors: Xiaoquan Qi, Zheyong Xue, Yingchun Zhang, Xia Xu, Dan Liu
  • Publication number: 20150013706
    Abstract: Two genes, A622 and NBB1, can be influenced to achieve a decrease of nicotinic alkaloid levels in plants. In particular, suppression of one or both of A622 and NBB1 may be used to decrease nicotine in tobacco plants.
    Type: Application
    Filed: July 28, 2014
    Publication date: January 15, 2015
    Inventors: Takashi HASHIMOTO, Akira Kato