The Polynucleotide Encodes An Inhibitory Rna Molecule Patents (Class 800/285)
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Patent number: 8455716Abstract: The present invention provides gene targets, constructs and methods for the genetic control of plant disease caused by multiple plant viruses. The present invention relates to achieving a plant protective effect through the identification of target coding sequences and the use of recombinant DNA technologies for post-transcriptionally repressing or inhibiting expression of the target coding sequences of plant-parasitic viruses. Protein-expression based approaches may also be utilized to augment phenotype resistance. Thus, transcription of a single transgenic event comprising one or more plant expression cassettes can allow for broad spectrum resistance of a plant to multiple plant viral strains and species among the geminiviruses, tospoviruses, and potexviruses.Type: GrantFiled: April 20, 2010Date of Patent: June 4, 2013Assignee: Monsanto Technology LLCInventors: Shihshieh Huang, Stanislaw Flasinski, Alessandra Frizzi, Brad Gabor, Charles Hagen, John Kao, Raquel Salati
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Patent number: 8455630Abstract: The invention provides polynucleotide and polypeptide sequences isolated from P. radiata and E. grandis that are involved in wood and cell wall biosynthesis. Methods for using the sequences, along with constructs and transgenic plants, are provided also.Type: GrantFiled: September 13, 2011Date of Patent: June 4, 2013Assignee: Arborgen Inc.Inventors: Leonard N. Bloksberg, Michael J. Frost, Richard Forster, Colleen Higgins, William H. Rottmann, Kim Norris-Caneda
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Publication number: 20130125259Abstract: Double-stranded RNA (dsRNA) induces sequence-specific post-transcriptional gene silencing in many organisms by a process known as RNA interference (RNAi). Using a Drosophila in vitro system, we demonstrate that 19-23 nt short RNA fragments are the sequence-specific mediators of RNAi. The short interfering RNAs (siRNAs) are generated by an RNase III-like processing reaction from long dsRNA. Chemically synthesized siRNA duplexes with overhanging 3? ends mediate efficient target RNA cleavage in the lysate, and the cleavage site is located near the center of the region spanned by the guiding siRNA. Furthermore, we provide evidence that the direction of dsRNA processing determines whether sense or antisense target RNA can be cleaved by the produced siRNP complex.Type: ApplicationFiled: December 21, 2012Publication date: May 16, 2013Applicants: Max-Planck-Gesellschaft zur Forderung der Wissenschaften E.V., University Of Massachusetts, Whitehead Institute For Biomedical Research, Massachusetts Institute Of TechnologyInventors: Max-Planck-Gesellschaft zur Forderung der Wissenschaften E.V., Massachusetts Institute Of Technology, Whitehead Institute For Biomedical Research, University Of Massachusetts
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Publication number: 20130125260Abstract: The invention provides constructs and methods of screening for constructs useful in conferring resistance in plants to pests by gene silencing. The invention also provides pest-resistant plants transformed with the present constructs. One screening method of the invention comprises the steps of: selecting at least one pest target nucleotide sequence, producing a plurality of dsRNA test agents that target the pest target nucleotide sequence, testing and scoring the plurality of dsRNA test agents for toxicity to the pest, and producing a silencing construct based on a superior-scoring test agent.Type: ApplicationFiled: January 14, 2013Publication date: May 16, 2013Applicant: VENGANZA INC.Inventor: VENGANZA INC.
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Publication number: 20130117886Abstract: The present invention relates to isolated nucleic acid molecules and their corresponding encoded polypeptides able confer the trait of modulated plant size, vegetative growth, organ number, plant architecture, sterility or seedling lethality in plants. The present invention further relates to the use of these nucleic acid molecules and polypeptides in making transgenic plants, plant cells, plant materials or seeds of a plant having such modulated growth or phenotype characteristics that are altered with respect to wild type plants grown under similar conditions.Type: ApplicationFiled: December 20, 2012Publication date: May 9, 2013Applicant: CERES, INC.Inventor: Ceres, Inc.
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Patent number: 8436235Abstract: The present invention relates to p450 enzymes and nucleic acid sequences encoding p450 enzymes in Nicotiana, and methods of using those enzymes and nucleic acid sequences to alter plant phenotypes.Type: GrantFiled: October 11, 2010Date of Patent: May 7, 2013Assignee: U.S. Smokeless Tobacco CompanyInventor: Dongmei Xu
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Patent number: 8420890Abstract: The present invention discloses transgenic plants having an altered level of NAP protein compared to that of a non-transgenic plant, where the transgenic plants display an altered leaf senescence phenotype relative to a non-transgenic plant, as well as mutant plants comprising an inactivated NAP gene, where mutant plants display a delayed leaf senescence phenotype compared to that of a non-mutant plant. The present invention also discloses methods for delaying leaf senescence in a plant, as well as methods of making a mutant plant having a decreased level of NAP protein compared to that of a non-mutant plant, where the mutant plant displays a delayed leaf senescence phenotype relative to a non-mutant plant. Methods for causing precocious leaf senescence or promoting leaf senescence in a plant are also disclosed. Also disclosed are methods of identifying a candidate plant suitable for breeding that displays a delayed leaf senescence and/or enhanced yield phenotype.Type: GrantFiled: March 28, 2007Date of Patent: April 16, 2013Assignee: Cornell Research Foundation, Inc.Inventors: Susheng Gan, Yongfeng Guo
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Patent number: 8415526Abstract: Isolated nucleic acid fragments comprising precursor miRNA, and artificial miRNAs and their use in down-regulating gene expression are described.Type: GrantFiled: January 11, 2012Date of Patent: April 9, 2013Assignee: E. I. du Pont de Nemours and CompanyInventor: Brian McGonigle
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Patent number: 8410335Abstract: A method for producing a rose characterized by artificially suppressing the rose endogenous metabolic pathway and expressing the pansy gene coding for flavonoid 3?,5?-hydroxylase.Type: GrantFiled: August 13, 2004Date of Patent: April 2, 2013Assignee: Suntory Holdings LimitedInventors: Yoshikazu Tanaka, Yuko Fukui, Junichi Togami, Yukihisa Katsumoto, Masako Mizutani
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Patent number: 8410334Abstract: This invention provides a non-natural transgenic plant cell expressing at least one invertebrate miRNA in planta for suppression of a target gene of an invertebrate pest or of a symbiont associated with the invertebrate pest, recombinant DNA constructs for expression of at least one invertebrate miRNA in planta and methods of use thereof, a non-natural transgenic plant containing the non-natural transgenic plant cell of this invention, a non-natural transgenic plant grown from the non-natural transgenic plant cell of this invention, and non-natural transgenic seed produced by the non-natural transgenic plants, as well as commodity products produced from a non-natural transgenic plant cell, plant, or seed of this invention.Type: GrantFiled: February 19, 2008Date of Patent: April 2, 2013Assignee: Monsanto Technology LLCInventors: Edwards Allen, William P. Donovan, Gregory R. Heck, James K. Roberts, Virginia Ursin, Yuanji Zhang
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Patent number: 8410341Abstract: The gene encoding N-methylputrescine oxidase (MPO) and constructs comprising such DNA are provided, including methods of regulating MPO expression independently or with other alkaloid biosynthesis genes to modulate alkaloid production in plants and host cells. MPO genes or fragments thereof are useful for reducing pyrrolidine or tropane alkaloid production in plants, for increasing pyrrolidine or tropane alkaloid production in plants, and for producing an MPO enzyme in host cells.Type: GrantFiled: June 19, 2007Date of Patent: April 2, 2013Assignee: National Research Council of CanadaInventors: Jonathan E. Page, Enwu Liu
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Patent number: 8410337Abstract: An isolated polypeptide containing an amino acid sequence at least 70% identical to a Tubby-like protein (SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or 11), and an isolated nucleic acid encoding the polypeptide. Disclosed is an isolated nucleic acid that, under stringent conditions, hybridizes to a probe containing one of SEQ ID NOs:1-11; or its complementary sequence. Also disclosed are (1) a transformed cell or a transgenic plant containing such a nucleic acid and (2) a transformed cell or a transgenic plant having a reduced level(s) of one or more of the Tubby-like proteins. Also within the scope of the invention are methods for making the transformed cells or transgenic plants.Type: GrantFiled: February 17, 2009Date of Patent: April 2, 2013Assignee: Academia SinicaInventors: Jei-Fu Shaw, Chia-ping Lai, Po-Hong Chen
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Patent number: 8404927Abstract: This invention discloses recombinant DNA constructs that transcribe to RNA having improved resistance to a plant RNase III enzyme, and methods for their use in making transgenic plant cells, plants, seeds, and products derived therefrom. Also provided are compositions and methods for imparting to a plant resistance to a pest or pathogen of the plant.Type: GrantFiled: June 13, 2006Date of Patent: March 26, 2013Assignee: Monsanto Technology LLCInventors: Edwards Allen, Larry A. Gilbertson, Elysia K. Krieger, James K. Roberts, Jeffrey M. Staub
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Patent number: 8404929Abstract: Compositions and methods for modulating expression of gene products are provided. Compositions comprise suppression cassettes that comprise a convergent promoter pair operably linked to a silencing element that, upon expression, is capable of decreasing the expression of one or more target polynucleotides of interest. Compositions of the invention also include transformed plants, plant cells, plant tissues, and plant seeds. Methods of transformation and regeneration of plants comprising the novel constructs are provided. The methods find use in regulating gene expression, particularly genes associated with agronomic traits of interest. Further provided are promoter sequences, cells, plants, and vectors comprising these promoter sequences and methods of their use.Type: GrantFiled: September 29, 2009Date of Patent: March 26, 2013Assignee: Pioneer Hi-Bred International, Inc.Inventors: Darren B. Gruis, Criag Hastings
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Patent number: 8404928Abstract: This invention discloses recombinant DNA constructs encoding phased small RNAs useful in regulating expression of one or more genes of interest. Also disclosed by this invention are transgenic plant cells, plants, and seeds containing a recombinant DNA construct of this invention.Type: GrantFiled: August 31, 2007Date of Patent: March 26, 2013Assignee: Monsanto Technology LLCInventors: Edwards Allen, Liang Guo, Sara E. Heisel, Sergey I. Ivashuta, Yuanji Zhang
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Publication number: 20130065761Abstract: Methods are provided for increasing the resistance of plants to hypoxic or anoxic conditions. Such methods may be applied to increase the penetrance of plant roots in the growth medium or into soil. The methods according to the invention may include providing plants with a stress tolerance gene. Similar effects can be obtained by applying chemical compounds, including neonicotinoid compounds, to the plants.Type: ApplicationFiled: February 22, 2012Publication date: March 14, 2013Applicant: BAYER CROPSCIENCE N.V.Inventors: MICHAEL METZLAFF, MARC DE BLOCK
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Patent number: 8395021Abstract: Soybean ubiquitin promoters is from the soybean SUBI-3 polyubiquitin gene and processes for expressing nucleic acids of interest in transgenic plants under the control of the soybean ubiquitin promoter pare described. Higher expression levels of the nucleic acids are achieved when the promoter is included.Type: GrantFiled: May 8, 2008Date of Patent: March 12, 2013Assignee: The Ohio State University Research FoundationInventors: John J. Finer, Robert A. Bouchard
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Patent number: 8395023Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.Type: GrantFiled: July 8, 2010Date of Patent: March 12, 2013Assignee: Monsanto Technology LLCInventors: Larry A. Gilbertson, Sara E. Heisel, Shihshieh Huang, Elysia K. Krieger
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Patent number: 8383889Abstract: Various embodiments are directed to transgenic plants, including transgenic tobacco plants and derivative seeds, genetically modified to impede the transport of Cadmium (Cd) from the root system to aerial portions of transgenic plants by reducing the expression levels of HMA-related transporters. Various embodiments are directed to transgenic tobacco plants genetically modified to stably express a RNAi construct encoding RNAi polynucleotides that enable the degradation of endogenous NtHMA RNA variants. Reduced expression of NtHMA transporters in transgenic plants results in substantially reduced content of Cadmium (Cd) in the leaf lamina. Various consumable products that are substantially free or substantially reduced in Cd content can be produced by incorporating leaves derived from transgenic tobacco plants modified to reduce the expression of NtHMA transporters.Type: GrantFiled: December 12, 2008Date of Patent: February 26, 2013Assignee: Philip Morris USA Inc.Inventors: Alec J. Hayes, Chengalrayan Kudithipudi, Rutger S. van der Hoeven
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Publication number: 20130042372Abstract: Embodiments of the present invention relate generally to new bioenergy crops and methods of creating new bioenergy crops. For example, genes encoding microRNAs (miRNAs) are used to create transgenic crops. In some embodiments, over-expression of miRNA is used to produce transgenic perennials, such as trees, with altered lignin content or composition. In some embodiments, the transgenic perennials are Populus spp. In some embodiments, the miRNA is a member of the miR156 family. In some embodiments, the gene is Zea mays Cg1.Type: ApplicationFiled: August 10, 2012Publication date: February 14, 2013Inventors: Richard MEILAN, Peter Marius RUBINELLI
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Patent number: 8367895Abstract: Methods and compositions are provided which employ a silencing element that, when ingested by a pest, are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. Target polynucleotides for specific protein classes and target polynucleotides set forth in SEQ ID NOS:1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or active variants or fragments thereof are provided, wherein a decrease in expression of one or more sequences in the target pest controls the pest. Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. Plants, plant part, bacteria and host cells comprising the silencing elements or an active variant or fragment thereof are also provided.Type: GrantFiled: January 9, 2009Date of Patent: February 5, 2013Assignees: Pioneer Hi-Bred International, Inc., E.I. du Pont de Nemours and CompanyInventors: Rafael Herrmann, Michael Lassner, Albert L. Lu, Mark Nelson, James K. Presnail, Janet A. Rice
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Patent number: 8362323Abstract: The invention relates to a method of generating or increasing a pathogen resistance in plants by reducing the expression of at least one Armadillo repeat polypeptide or a functional equivalent thereof. The invention relates to novel nucleic acid sequences coding for a Hordeum vulgare Armadillo repeat (HvARM) polynucleotide and describes homologous sequences (ARM1) thereof, and to their use in methods for obtaining a pathogen resistance in plants e, and to nucleic acid constructs, expression cassettes and vectors which comprise these sequences and which are suitable for mediating a fungal resistance in plants. The invention furthermore relates to transgenic organisms, in particular plants, which are transformed with these expression cassettes or vectors, and to cultures, parts or transgenic propagation material derived therefrom.Type: GrantFiled: October 27, 2006Date of Patent: January 29, 2013Assignee: BASF Plant Science GmbHInventors: Dimitar Douchkov, Patrick Schweizer, Uwe Zierold
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Publication number: 20130022707Abstract: The present invention is directed to compositions and methods for altering the levels of seed proteins in cereal grain. The invention is directed to the alteration of seed protein levels in plant grain, resulting in grain with increased digestibility/nutrient availability, improved amino acid composition/nutritional value, increased response to feed processing, improved silage quality, and increased efficiency of wet milling.Type: ApplicationFiled: September 14, 2012Publication date: January 24, 2013Applicant: Pioneer Hi Bred International, Inc.Inventors: Rudolf Jung, Wang-Nan Hu, Robert B. Meeley, Vincent J.H. Sewalt, Ramesh Nair
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Publication number: 20130007913Abstract: A eukaryotic input circuit: computationally designed receptors, synthetic eukaryotic signal transduction pathways, and a synthetic signal sensitive promoter that allow highly specific transcriptional induction in response to an externally provided ligand is disclosed. The input circuit is able to specifically bind a targeted substance and transmit a signal to the nucleus where transcription of a gene is activated. An output circuit serves as a simple readout system of the substance detected by the input circuit. The readout circuit exemplified here is a degreening circuit which causes plants to turn white. Activation of the degreening circuit can be detected by eye, or remotely with a variety of machines (hand-held, aircraft or satellite based) and is also resettable. When linked the input circuit if operably linked to the output circuit, produces a functional plant detector.Type: ApplicationFiled: April 2, 2012Publication date: January 3, 2013Inventors: June Medford, Mauricio S. Antunes
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Patent number: 8338665Abstract: Methods of, and compositions for, assembling one or more transcription units in a genome without a linked selectable marker or other unwanted transcription unit are provided. Also provided methods of, and compositions for, assembling one or more transcription units in a genome with a reduced frequency of vector backbone.Type: GrantFiled: July 16, 2009Date of Patent: December 25, 2012Assignee: Monsanto Technology LLCInventors: Larry Gilbertson, Susan Jane Johnson
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Publication number: 20120322122Abstract: The invention provides methods for modifying lignin content and composition in plants and achieving associated benefits therefrom involving altered expression of newly discovered MYB4 transcription factors. Nucleic acid constructs for modifying MYB4 transcription factor expression are described. By over-expressing the identified MYB4 transcription factors, for example, an accompanying decrease in lignin content may be achieved. Plants are provided by the invention comprising such modifications, as are methods for their preparation and use.Type: ApplicationFiled: May 2, 2012Publication date: December 20, 2012Inventors: Hui Shen, Fang Chen, Richard A. Dixon
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Patent number: 8334430Abstract: This invention provides methods for producing a non-natural hybrid seed. Also disclosed are specific miRNAs and miRNA recognition sites useful for conferring inducible sterility on a crop plant, and recombinant DNA construct including such exogenous miRNA recognition sites.Type: GrantFiled: September 20, 2006Date of Patent: December 18, 2012Assignee: Monsanto Technology LLCInventors: Edwards M. Allen, Larry A. Gilbertson, Nancy M. Houmard, Shihshieh Huang, Sergey I. Ivashuta, James K. Roberts
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Publication number: 20120272406Abstract: The present invention relates to methods for increasing digestibility and/or crude protein of a plant by modifying lignin biosynthesis of the plant. The methods involve the manipulation of the expression level of genes in the lignin biosynthesis pathway by directly reducing the expression of genes using miRNA or using regulation of transcription factors. Expression cassettes for achieving such gene expression manipulation and transgenic plant cells and plants comprising the constructs and cassettes are also provided. Transcription regulating nucleotide sequences for use in the expression cassettes have also been developed as well as methods of using these sequences and cassettes.Type: ApplicationFiled: March 29, 2012Publication date: October 25, 2012Applicant: BASF Plant Science Company GmbHInventors: Hanping Guan, Beomseok Seo, Peifeng Ren, David Deppong, Huihua Fu
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Patent number: 8293974Abstract: A DNA polynucleotide is described herein having a modified sequence of a target gene, wherein any one type of nucleotide in the target gene sequence has been chemically modified to another type of nucleotide; and a complementary sequence of the unmodified target gene; wherein either one of the modified sequence or the complementary sequence is in a reverse orientation to the other sequence; and wherein the RNA sequence transcribed from the DNA polynucleotide forms a duplex between the modified sequence and the complementary sequence so that a long double stranded RNA (IdsRNA) duplex forms between the modified and complementary sequences with base pair mismatches where the nucleotides have been modified, the IdsRNA duplex being capable of inhibiting expression of the target gene. RNA polynucleotides and IdsRNA duplexes transcribed by the DNA polynucleotide are also described, as is a method for producing the IdsRNA duplexes. These IdsRNA duplexes can be used in gene silencing.Type: GrantFiled: January 29, 2008Date of Patent: October 23, 2012Assignee: University of WitwatersrandInventors: Patrick Arbuthnot, Marc Saul Weinberg, Marie Emma Christine Rey, Johan Harmse, Sarah Helen Taylor
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Patent number: 8293973Abstract: The present disclosure concerns methods and compositions relating to UXS polypeptides and/or nucleic acids encoding UXS polypeptides. In certain claims, the methods and compositions are of use to improve digestibility and/or ease of grain processing. Such improvements relate to a modulation in arabinoxylan and/or hemicellulose content in transgenic plants. Such plants can, for example, comprise one or more nucleic acid sequences that inhibit expression of one or more UDP-Xylose Synthase (UXS) genes.Type: GrantFiled: August 2, 2011Date of Patent: October 23, 2012Assignee: Pioneer Hi Bred International IncInventors: Xiaoming Bao, George W Singletary, Deborah J Wetterberg, Ramesh Nair, Kanwarpal S Dhugga, Matthias Liebergesell, David A Selinger
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Patent number: 8288613Abstract: The invention provides an enzyme having a lignan-hydroxylating activity, particularly an enzyme capable of catalyzing the reaction of transferring a hydroxyl group to a lignan, an enzyme capable of catalyzing the hydroxylation of piperitol to 9-hydroxylpiperitol or pinoresinol to 9-hydroxylpinoresinol. The invention provides a polypeptide having a lignan-hydroxylating activity; a polynucleotide encoding the same; a vector or transformant comprising the polynucleotide; a method for producing a polypeptide having a lignan-hydroxylating activity which comprises using the transformant; and so on. The transformant wherein the polynucleotide is expressibly introduced is useful for the hydroxylation of a lignan or for the production of a product using the same in the food sector and a variety of industry sectors.Type: GrantFiled: December 17, 2008Date of Patent: October 16, 2012Assignee: Suntory Holdings LimitedInventors: Eiichiro Ono, Asako Okada, Yuko Fukui
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Patent number: 8288614Abstract: The present invention is directed to compositions and methods for altering the levels of seed proteins in cereal grain. The invention is directed to the alteration of seed protein levels in plant grain, resulting in grain with increased digestibility/nutrient availability, improved amino acid composition/nutritional value, increased response to feed processing, improved silage quality, and increased efficiency of wet milling.Type: GrantFiled: January 8, 2010Date of Patent: October 16, 2012Assignee: Pioneer Hi Bred International, IncInventors: Rudolf Jung, Wang-Nan Hu, Robert B. Meeley, Vincent J.H. Sewalt, Ramesh Nair
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Patent number: 8278507Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: May 27, 2010Date of Patent: October 2, 2012Assignees: Novozymes, Inc., Novozymes, A/SInventors: Ye Liu, Lan Tang, Paul Harris, Wenping Wu
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Patent number: 8273950Abstract: Polynucleotides encoding polypeptides that comprise the biosynthetic pathway for phenylpropanoids and flavonoids in the coffee plant are disclosed. Also disclosed are methods for using these polynucleotides and polypeptides for the manipulation of flavor, aroma, and other features of coffee beans, as well as the manipulation resistance to pathogen, herbivore, and insect attack in the coffee plant.Type: GrantFiled: October 13, 2006Date of Patent: September 25, 2012Assignees: Nestec S. A., Cornell UniversityInventors: Maud Lepelley, Gerald Cheminade, James GĂ©rard McCarthy, Vincent Petiard, Chenwei Lin, Steven D. Tanksley
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Patent number: 8273951Abstract: Isolated nucleic acid fragments comprising precursor miRNA, and artificial miRNAs and their use in down-regulating gene expression are described.Type: GrantFiled: January 11, 2012Date of Patent: September 25, 2012Assignee: E. I. du Pont de Nemours and CompanyInventor: Brian McGonigle
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Patent number: 8273949Abstract: The present invention relates to a method for identifying and isolating native plant nucleic acid sequences that may function as T-DNAs or T-DNA border-like sequences, effecting the transfer of one polynucleotide into another polynucleotide. The present invention also provides a modified tuber, such as a genetically modified mature tuber, that comprises at least one trait that is not exhibited by a non-modified tuber of the same species.Type: GrantFiled: June 25, 2004Date of Patent: September 25, 2012Assignee: J.R. Simplot CompanyInventors: Caius Rommens, Jingsong Ye, Jaime Menendez-Humara, Hua Yan, Kathy Swords
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Publication number: 20120240290Abstract: The present invention provides compositions and methods for modulating flowering time in plants. Maize RAP2.7 nucleotide sequences are disclosed which upon overexpression cause later flowering and when inhibited cause earlier flowering. Also disclosed is a DNA sequence which acts as a regulator/enhancer of RAP2.7, termed VGT1. This sequence does not code for any known protein, but acts as either a RNAi element or a regulatory DNA or RNA element that either directly regulates expression of flowering genes such as Rap2.7 or specifically targets expression of other genes which control flowering genes such as Rap2.7. This element this can be used as a sequence—based marker to identify inbred and hybrids which have altered maturity. Methods for expressing these nucleotide sequences in a plant for modifying maturity and flowering in plants are provided as well as expression constructs, vectors, transformed cells and plants.Type: ApplicationFiled: March 27, 2012Publication date: September 20, 2012Applicant: PIONEER HI BRED INTERNATIONAL INCInventors: Dwight Tomes, Silvio Salvi, Michele Morgante, Giorgio Sponza, Edward Bruggemann, Xiaomu Niu, Bailin Li, Roberto Tuberosa
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Patent number: 8252977Abstract: Polynucleotides encoding polypeptides that comprise the biosynthetic pathway for carotenoids and apocarotenoids in the coffee plant are disclosed. Also disclosed are a promoter sequence from a coffee carotenoid gene, and methods for using these polynucleotides, polypeptides, and promoter sequences for gene regulation and the manipulation of flavor, aroma, and other features of coffee beans, as well as the manipulation of photosynthesis in the coffee plant.Type: GrantFiled: September 1, 2006Date of Patent: August 28, 2012Assignees: Nestec S. A., Cornell UniversityInventors: Steven D. Tanksley, Chenwei Lin, Andrew Simkin, James GĂ©rard McCarthy, Vincent Petiard
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Patent number: 8252974Abstract: The present invention relates to a new plant breeding process. The process improves the agronomic performance of crop plants by using genetic material that is also used in classical breeding. Instead of sexually recombining entire genomes at random, as is done in classical breeding, specific genetic elements are rearranged in vitro and inserted back into individual plant cells. Plants obtained through this new plant breeding process do not contain foreign nucleic acid but only contain nucleic acid from the plant species selected for transformation or plants that are sexually compatible with the selected plant species. Plants developed through this new plant breeding process are provided. In particular, potato plants displaying improved tuber storage and health characteristics are provided.Type: GrantFiled: May 6, 2009Date of Patent: August 28, 2012Assignee: J.R. Simplot ComanyInventor: Caius Rommens
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Patent number: 8242327Abstract: A method for efficiently producing homozygous organisms from a heterozygous non-human starting organism, comprising providing of a heterozygous starting organism; allowing the starting organism to produce haploid cells; creating homozygous organisms from the haploid cells thus obtained; and selecting the organisms having the desired set of chromosomes, wherein during production of the haploid cells no recombination occurs in order to obtain a limited number of genetically different haploid cells. Recombination can also be prevented or suppressed.Type: GrantFiled: August 23, 2002Date of Patent: August 14, 2012Assignee: Rijk Zwaan Zaadteelt En Zaadhandel B.V.Inventors: Robert Helene Ghislain Dirks, Cornelis Maria Petrus Van Dun, Kornelius Reinink, Jacobus Petrus Cornelis De Wit
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Patent number: 8237017Abstract: Naturally occurring and synthetic novel stress-related miRNAs are provided which can be used to modify the stress tolerance of plants.Type: GrantFiled: May 10, 2007Date of Patent: August 7, 2012Assignee: Bayer CropScience NVInventors: Marc Bots, Michael Metzlaff
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Patent number: 8232380Abstract: Regulatory regions suitable for directing the expression of a heterologous polynucleotide under light conditions in which the red/far red ratio is less than 1 are described, as well as nucleic acid constructs that include these regulatory regions. Also disclosed are transgenic plants that contain such constructs and methods of producing such transgenic plants.Type: GrantFiled: August 25, 2008Date of Patent: July 31, 2012Assignee: Ceres, Inc.Inventors: Shing Kwok, Amy Jo Miyamoto, Kenneth Bounds
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Patent number: 8217227Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.Type: GrantFiled: October 10, 2006Date of Patent: July 10, 2012Assignee: Monsanto Technology LLCInventors: Edwards Allen, Larry A. Gilbertson, Shihshieh Huang, Thomas Malvar, James K. Roberts
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Patent number: 8207399Abstract: The present invention relates to a method for producing syringyl lignin in gymnosperms. The production of syringyl lignin in gymnosperms is accomplished by genetically transforming a gymnosperm genome, which does not normally contain genes which code for enzymes necessary for production of syringyl lignin, with DNA which codes for enzymes found in angiosperms associated with production of syringyl lignin. The expression of the inserted DNA is mediated using host promoter regions in the gymnosperm.Type: GrantFiled: June 24, 2010Date of Patent: June 26, 2012Assignee: Arborgen Inc.Inventors: Vincent L. Chiang, Daniel T. Carraway, Richard H. Smeltzer
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Publication number: 20120157512Abstract: Methods and compositions for transforming plants to express polynucleotides capable of gene silencing gene expression in pathogens of beneficial insets such as IAPV, Nosema species and Varroa mites, and methods for using the transgenic plants for reducing infection and susceptibility of bees to Colony Collapse Disorder are provided.Type: ApplicationFiled: August 22, 2010Publication date: June 21, 2012Applicant: MONSANTO TECHNOLOGY LLCInventors: Eyal Ben-Chanoch, Nitzan Paldi, Gal Yarden
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Publication number: 20120144527Abstract: The invention provides methods and compositions useful in target sequence suppression and target sequence validation. The invention provides polynucleotide constructs useful for gene silencing, as well as cells, plants and seeds comprising the polynucleotides. The invention also provides a method for using microRNA to silence a target sequence.Type: ApplicationFiled: December 18, 2007Publication date: June 7, 2012Inventor: Milo J. Aukerman
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Patent number: 8193412Abstract: The present invention relates to a new plant breeding process. The process improves the agronomic performance of crop plants by using genetic material that is also used in classical breeding. Instead of sexually recombining entire genomes at random, as is done in classical breeding, specific genetic elements are rearranged in vitro and inserted back into individual plant cells. Plants obtained through this new plant breeding process do not contain foreign nucleic acid but only contain nucleic acid from the plant species selected for transformation or plants that are sexually compatible with the selected plant species. Plants developed through this new plant breeding process are provided. In particular, potato plants displaying improved tuber storage and health characteristics are provided.Type: GrantFiled: January 31, 2007Date of Patent: June 5, 2012Assignee: J.R. Simplot CompanyInventors: Caius Rommens, Jingsong Ye, Hua Yan
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Patent number: 8188337Abstract: The present invention relates to p450 enzymes and nucleic acid sequences encoding p450 enzymes in Nicotiana, and methods of using those enzymes and nucleic acid sequences to alter plant phenotypes.Type: GrantFiled: September 23, 2011Date of Patent: May 29, 2012Assignee: U.S. Smokeless Tobacco CompanyInventor: Dongmei Xu
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Patent number: 8188336Abstract: Rice having reduced levels of starch branching enzymes produce grain having a high relative amylose content in the endosperm. The rice grain of this invention can be of a non-shrunken phenotype despite a lesion in the amylopectin synthesis pathway and may be transgenic or nontransgenic.Type: GrantFiled: August 6, 2010Date of Patent: May 29, 2012Assignee: Commonwealth Scientific and Industrial Research OrganisationInventors: Zhongyi Li, Matthew Kennedy Morell, Sadequr Rahman
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Patent number: 8173867Abstract: The present invention provides compositions and methods for modulating flowering time in plants. Maize RAP2.7 nucleotide sequences are disclosed which upon overexpression cause later flowering and when inhibited cause earlier flowering. Also disclosed is a DNA sequence which acts as a regulator/enhancer of RAP2.7, termed VGT1. This sequence does not code for any known protein, but acts as either a RNAi element or a regulatory DNA or RNA element that either directly regulates expression of flowering genes such as Rap2.7 or specifically targets expression of other genes which control flowering genes such as Rap2.7. This element this can be used as a sequence-based marker to identify inbred and hybrids which have altered maturity. Methods for expressing these nucleotide sequences in a plant for modifying maturity and flowering in plants are provided as well as expression constructs, vectors, transformed cells and plants.Type: GrantFiled: November 24, 2008Date of Patent: May 8, 2012Assignee: Pioneer Hi Bred International IncInventors: Dwight T. Tomes, Silvio Salvi, Michele Morgante, Giorgio Sponza, Edward Bruggemann, Xiaomu Niu, Bailin Li, Roberto Tuberosa