Abstract: The present disclosure provides methods, recombinant DNA molecules, recombinant host cells containing the DNA molecules, and transgenic plant cells, plant tissue and plants which contain and express at least one antisense or interference RNA specific for a thiamine biosynthetic coding sequence or a thiamine binding protein or a thiamine-degrading protein, wherein the RNA or thiamine binding protein is expressed under the regulatory control of a transcription regulatory sequence which directs expression in male and/or female reproductive tissue. These transgenic plants are conditionally sterile; i.e., they are fertile only in the presence of exogenous thiamine. Such plants are especially appropriate for use in the seed industry or in the environment, for example, for use in revegetation of contaminated soils or phytoremediation, especially when those transgenic plants also contain and express one or more chimeric genes which confer resistance to contaminants.

Abstract: The invention provides methods for decreasing lignin content and improving lignin profiles. Also provided are the plants prepared by the methods of the invention. Such plants may exhibit improved digestibility relative to prior plants.

Abstract: The present invention concerns a method of genetic modification of a TGB-3 wild type viral sequence for reducing or suppressing the possible deleterious effects of the agronomic properties of a transformed plant or plant cell by said TGB-3 viral sequence. The invention further relates to genetically modified TGB-3 viral sequences suitable to induce gene silencing. In particular hairpin constructs based on such sequences proved highly efficient to induce a PTGS mechanism and degradation of the whole of RNA2 thereby. When plants are transformed accordingly the spread of the virus in the plant is significantly reduced or blocked.

Abstract: A switchable photosystem-II designer algae for photobiological hydrogen production. The designer transgenic algae includes at least two transgenes for enhanced photobiological H2 production wherein a first transgene serves as a genetic switch that can controls photosystem II (PSII) oxygen evolution and a second transgene encodes for creation of free proton channels in the algal photosynthetic membrane. In one embodiment, the algae includes a DNA construct having polymerase chain reaction forward primer (302), a inducible promoter (304), a PSII-iRNA sequence (306), a terminator (308), and a PCR reverse primer (310). In other embodiments, the PSII-iRNA sequence (306) is replaced with a CF1-iRNA sequence (312), a streptomycin-production gene (314), a targeting sequence (316) followed by a proton-channel producing gene (318), or a PSII-producing gene (320). In one embodiment, a photo-bioreactor and gas-product separation and utilization system produce photobiological H2 from the switchable PSII designer alga.

Abstract: A process of producing a transgenic multi-cellular plant or animal organism expressing a trait of interest and having a controlled distribution of said trait to progeny, wherein said process comprises hybridising a first multi-cellular organism or a cell thereof having a first heterologous DNA sequence comprising a first fragment of a nucleotide sequence encoding said trait of interest and a second multi-cellular organism or a cell thereof having a second heterologous DNA sequence comprising a second fragment of the nucleotide sequence encoding said trait of interest, whereby said first and said second heterologous sequences are designed such that said trait of interest arises due to RNA trans-splicing after said hybridation.

Abstract: The present invention relates to plant cells and plants, which are genetically modified, wherein the genetic modification leads to the reduction of the activity of a Class 3 vegetable branching enzyme in comparison with corresponding wild type plant cells or wild type plants that have not been genetically modified. Furthermore, the present invention relates to means and methods for the manufacture of such plant cells and plants. Plant cells and plants of this type synthesise a modified starch. The present invention therefore also relates to the starch synthesised by the plant cells and plants according to the invention as well as to methods for the manufacture of the starch and to the manufacture of starch derivatives of this starch. Furthermore, the present invention relates to nucleic acids coding a Class 3 branching enzyme, vectors, host cells, plant cells and plants containing such nucleic acid molecules.

Abstract: A nucleic acid which encodes a peroxisomal fatty acid transporter, uses thereof and a method of genetic manipulation of peroxisomal fatty acid transport and/or Metabolism. The nucleic acid and its products are especially for use in regulation of peroxisomal fatty acid transport in plant and in controlling the spectrum of fatty acids which can be utilised by the plant.

Abstract: The present invention concerns double stranded RNA compositions and transgenic plants capable of inhibiting expression of essential genes in parasitic nematodes, and methods associated therewith. Specifically, the invention relates to the use of RNA interference to inhibit expression of a target essential parasitic nematode gene selected from the group consisting of a parasitic nematode cytosolic chaperonin gene, a parasitic nematode gene encoding heat shock protein-90, a parasitic nematode gene homologous to the C. elegans Y65B4BR.5a gene, and a parasitic nematode gene homologous to a C. elegans pat-10 gene, and relates to the generation of plants that have increased tolerance to parasitic nematodes.

Abstract: A process is provided of introducing an RNA into a living cell to inhibit gene expression of a target gene in that cell. The process may be practiced ex vivo or in vivo. The RNA has a region with double-stranded structure. Inhibition is sequence-specific in that the nucleotide sequences of the duplex region of the RNA and of a portion of the target gene are identical. The present invention is distinguished from prior art interference in gene expression by antisense or triple-strand methods.

Abstract: The present invention discloses transgenic plants having an altered level of NAP protein compared to that of a non-transgenic plant, where the transgenic plants display an altered leaf senescence phenotype relative to a non-transgenic plant, as well as mutant plants comprising an inactivated NAP gene, where mutant plants display a delayed leaf senescence phenotype compared to that of a non-mutant plant. The present invention also discloses methods for delaying leaf senescence in a plant, as well as methods of making a mutant plant having a decreased level of NAP protein compared to that of a non-mutant plant, where the mutant plant displays a delayed leaf senescence phenotype relative to a non-mutant plant. Methods for causing precocious leaf senescence or promoting leaf senescence in a plant are also disclosed. Also disclosed are methods of identifying a candidate plant suitable for breeding that displays a delayed leaf senescence and/or enhanced yield phenotype.

Abstract: The present invention is directed to an isolated starch, a transgenic plant or plant part producing the starch, flour and a thickened foodstuff prepared from a grain capable of producing such isolated starch. The present invention is directed to a method for altering starch amylose composition of a cereal grain.

Abstract: The invention provides Bean pod mottle virus (BPMV) vectors useful for expression of heterologous proteins in plants such as soybean. The BPMV vectors are also useful for virus-induced gene silencing. The invention also provides methods for expressing a heterologous polypeptide in a plant such as soybean. The invention additionally provides methods for virus-induced gene silencing, particularly in a soybean plant, which can be used to determine the function of a gene of interest.

Abstract: This invention relates to newly identified polynucleotides and polypeptides in the phytic acid biosynthetic pathway, variants and derivatives of same; methods for making the polynucleotides, polypeptides, variants, derivatives and antagonists. In particular the invention relates to polynucleotides and polypeptides of the inositol polyphosphate kinase gene family. In particular this invention relates to using the newly identified polynucleotides and polypeptides to modulate phytic acid biosynthesis in such a way as to decrease phytate and/or increase non-phytate phosphorous in plants.

Abstract: Maize plants with reduced gene silencing are disclosed.

Abstract: In one aspect, the invention provides novel tetraploid Brassica plants having no more than two expressible FAD2 coding sequences, capable of producing canola quality oils. Other aspects of the invention provide new variants of the FAD2 enzyme, comprising BjFAD2-b and BjFAD2-a, as well as nucleic acid sequences encoding such peptides. Other aspects of the invention include nucleic acid sequences upstream from the BjFAD2-b or BjFAD2-a ORFs. Other aspects of the invention include transgenic plants and plant pads. Vectors capable of transforming plant cells are provided, comprising the nucleic acids of the invention, including FAD2 coding sequences. Corresponding methods are provided for obtaining the transgenic plants of the invention. Methods are provided for using the plants of the invention, including selected plants and transgenic plants, to obtain plant products.

Abstract: DNA encoding a plant quinolate phosphoribosyl transferase (QPRTase) enzyme, and constructs comprising such DNA are provided. Methods of altering quinolate phosphoribosyl transferase expression are provided.

Abstract: The present invention is in the field of plant genetics and provides recombinant nucleic acid molecules, constructs, and other agents associated with the coordinate manipulation of multiple genes in the fatty acid synthesis pathway. In particular, the agents of the present invention are associated with the simultaneous enhanced expression of certain genes in the fatty acid synthesis pathway and suppressed expression of certain other genes in the same pathway. Also provided are plants incorporating such agents, and in particular plants incorporating such constructs where the plants exhibit altered seed oil compositions.

Abstract: Methods for altering levels in plants of one or more phenolic compounds that are intermediates or final products of the plant phenylpropanoid pathway are provided. One method comprises transforming a plant cell with an expression construct comprising a nucleic acid which encodes a transactivator protein comprising the myb domain of the maize “ZmMyb-IF35” protein and an activation domain. Another method comprises transforming a plant cell with an expression construct comprising a transgene which encodes an antisense ZmMyb-IF35 RNA. The present invention also relates to expression constructs and vectors used in the present methods, transformed plant cells and transgenic plants prepared according to the present methods, and the seeds of such transgenic plants.

Abstract: Methods and kits for determining the specificity of siRNAs for their targets are provided. Also provided is a method for performing genetic analysis of the target protein or gene using different versions of a synthetic gene to complement the phenotype induced by RNAi-mediated silencing of the target protein and/or gene of interest. Finally, a method for treating genetic disorders associated with production of mutated proteins is also disclosed.

Abstract: The present disclosure concerns methods and compositions relating to UXS polypeptides and/or nucleic acids encoding UXS polypeptides. In certain claims, the methods and compositions are of use to improve digestibility and/or ease of grain processing. Such improvements relate to a modulation in arabinoxylan and/or hemicellulose content in transgenic plants. Such plants can, for example, comprise one or more nucleic acid sequences that inhibit expression of one or more UDP-Xylose Synthase (UXS) genes.

Abstract: Expressed Sequence Tags (ESTs) isolated from lily are disclosed. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The disclosed ESTs have utility in the development of new strategies for understanding critical plant developmental and metabolic pathways. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. Sequence homology analyses using the ESTs provided in the present invention, will result in more efficient gene screening for desirable agronomic traits. An expanding database of these select pieces of the plant genomics puzzle will quickly expand the knowledge necessary for subsequent functional validation, a key limitation in current plant biotechnology efforts.

Abstract: Nucleic acid molecules are described encoding a starch granule-bound protein as well as methods and recombinant DNA molecules for the production of transgenic plant cells and plants synthesizing a modified starch with modified viscosity properties and a modified phosphate content. Moreover, the plant cells and plants resulting from those methods as well as the starch obtainable therefrom are described.

Abstract: Rice phyC mutants were isolated using a mutant panel isolation method. When the mutants were grown under long-day photoperiodic conditions, it was found that they flowered (exposed their panicles (heads)) about one week earlier than the control rice. The results indicate that suppression of PHYC gene expression can promote plant flowering under long-day conditions. Utilization of the PHYC gene for promoting plant flowering will contribute substantially to breed improvement, for example, by facilitating the creation of useful agricultural crops and decorative plants that have a new characteristic adaptable for other cultivation areas and times. The rice phyC mutants described herein, which promote flowering under long-day conditions, will be highly prized as a new early-harvest rice cultivar.

Abstract: The present invention is in the field of plant genetics and provides recombinant nucleic acid molecules, constructs, and other agents associated with the coordinate manipulation of multiple genes in the fatty acid synthesis pathway. In particular, the agents of the present invention are associated with the simultaneous enhanced expression of certain genes in the fatty acid synthesis pathway and suppressed expression of certain other genes in the same pathway. Also provided are plants incorporating such agents, and in particular plants incorporating such constructs where the plants exhibit altered seed oil compositions.

Abstract: Various embodiments are directed to transgenic plants, including transgenic tobacco plants and derivative seeds, genetically modified to impede the transport of Cadmium (Cd) from the root system to aerial portions of transgenic plants by reducing the expression levels of HMA-related transporters. Various embodiments are directed to transgenic tobacco plants genetically modified to stably express a RNAi construct encoding RNAi polynucleotides that enable the degradation of endogenous NtHMA RNA variants. Reduced expression of NtHMA transporters in transgenic plants results in substantially reduced content of Cadmium (Cd) in the leaf lamina. Various consumable products that are substantially free or substantially reduced in Cd content can be produced by incorporating leaves derived from transgenic tobacco plants modified to reduce the expression of NtHMA transporters.

Abstract: The present invention provides methods and compositions directed to the production of polypeptides in plant protein storage tissue. Methods of the invention include methods for producing polypeptides in protein storage tissues by decreasing the level of protease activity in the plant protein storage tissue. Compositions of the invention include plants that are genetically modified such that the activity of one or more proteases is reduced or eliminated, transformed seeds obtained from these genetically modified plants, and sequences encoding a novel member of the VPE cysteine protease family found in seed, ?-VPE. These methods and compositions find utility in altering the nutritional or other qualities of seed, and in producing useful proteins including pharmacological or industrial proteins.

Abstract: The invention relates to transgenic expression cassettes for expressing two nucleic acid sequences in a plant cell comprising at least one regulatory sequence selected from the group consisting of a) the promoter shown in SEQ ID NO: 1 or 2, b) functional equivalents of the promoter shown in SEQ ID NO: 1 or 2 which have an identity of at least 80% to the sequence shown in SEQ ID NO: 1 or 2 and which have substantially the same promoter activity as the promoter shown in SEQ ID NO: 1 or 2, c) functional equivalents of the promoter shown in SEQ ID NO: 1 or 2 which comprise at least 25 consecutive nucleotides of the sequences shown in SEQ ID NO: 1 or 2 and which have substantially the same promoter activity as the promoter shown in SEQ ID NO: 1 or 2, and d) functionally equivalent fragments of sequences a) or b) or c), which have at least 25 consecutive nucleotides of said sequences a) or b) or c) and have substantially the same promoter activity as the promoter shown in SEQ ID NO: 1 or 2, where said regulatory

Abstract: This invention relates to methods for knock-down of a target gene in plants, particularly efficient and specific methods for knock-down of a target gene in plants. This invention also relates to methods for silencing endogenous plant genes or plant pathogen genes. It further relates to nucleic acid constructs (DNA, RNA) which comprise a nucleic acid sequence that corresponds to a target gene or fragment thereof flanked by two complementary sites to an smRNA, e.g., a miRNA (one complementary site is on either side of the nucleic acid sequence), resulting in, for example the configuration: complementary site—nucleic acid sequence that corresponds to a target gene—complementary site.

Abstract: The invention provides genetically engineered, preselected DNA sequences and methods of using them to alter the nutritional content of plant seed. Methods of the invention are directed to increasing the weight percent of at least one amino acid essential to the diet of animals, or increasing the starch content, of a plant. One such method involves stably transforming a cell of a plant with an a preselected DNA sequence encoding an RNA molecule substantially identical or complementary to a messenger RNA (mRNA) encoding a plant seed storage protein, preferably a seed storage protein which is deficient in at least one amino acid essential to the diet of animals. An alternative method employs stably transforming cells with at least two preselected DNA sequences, one of which encodes an RNA molecule substantially identical or complementary to a messenger RNA (mRNA) encoding a plant seed storage protein, and the other preselected DNA molecule which encodes a preselected polypeptide.

Abstract: The promoter of a soybean translation elongation factor EF1 alpha and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in a tissue-independent or constitutive manner in plants are described.

Abstract: The present invention relates to a method for identifying and isolating native plant nucleic acid sequences that may function as T-DNAs or T-DNA border-like sequences, effecting the transfer of one polynucleotide into another polynucleotide. The present invention also provides a modified tuber, such as a genetically modified mature tuber, that comprises at least one trait that is not exhibited by a non-modified tuber of the same species.

Abstract: The present invention is directed to a DNA construct which includes a modified DNA molecule with a nucleotide sequence which is at least 80%, but less than 100%, homologous to two or more desired trait DNA molecules and which imparts the desired trait to plants transformed with the DNA construct. Each of the desired trait DNA molecules relative to the modified nucleic acid molecule have nucleotide sequence similarity values which differ by no more than 3 percentage points. The DNA construct may further include either a silencer or a plurality of modified DNA molecules. The present invention also relates to host cells, plant cells, transgenic plants, and transgenic plant seeds containing such DNA constructs.

Abstract: The present application provides Brassica INDEHISCENT1 (BIND) sequences.

Abstract: Barley having a reduced level of SBEIIa activity produces grain having a high relative amylose content. The barley might additionally have reduced levels of SBEIIb activity. The barley grain of this invention can be of a non-shrunken phenotype despite a lesion in the amylopectin synthesis pathway.

Abstract: The invention relates to transgenic plants and plant cells comprising a reduced expression of invertase inhibitors. The modification of the expression of the invertase inhibitors is achieved by introducing a cDNA sequence in an antisense orientation with respect to a promoter. The expression of the antisense DNA sequence is under the regulation of either the CaMV35S promoter or a tissue specific promoter.

Abstract: This invention is directed to a monopartite RNA viral vector comprising modified tobravirus RNA-1 comprising an inserted foreign RNA sequence. This invention is also directed to a bipartite RNA viral vector derived from a tobravirus, wherein the vector comprises one or more foreign RNA sequences. The invention is directed to a method of silencing one or more endogenous plant host genes and a method of simultaneously silencing a plant host gene and expressing a foreign gene in a plant host. Such methods comprise infecting a plant host with a bipartite vector comprising modified tobravirus RNA-1 and RNA-2. The invention is further directed to a method of compiling a plant functional gene profile, a method of changing the phenotype or biochemistry of a plant host, and a method of determining the presence of a trait in a plant host, using a monopartite or bipartite viral vector derived from a tobravirus.

Abstract: The invention provides improved methods and means for introducing inhibitory RNA into plant cells. The invention also provides kits comprising viral RNA vectors derived from satellite viruses and corresponding helper viruses for the introduction of inhibitory RNA into plant cells and plants. Further, the invention comprises methods for obtaining an enhanced or improved gene-silencing phenotype.

Abstract: The invention provides novel methods and compositions for modulating gene function in plants. In particular, the invention provides methods and compositions that allow efficient induction of virus-induced gene silencing in plants. The invention is significant in that it allows high throughput analysis of gene function in plants.

Abstract: The present invention relates to methods for generating resistance against Cucumber Green Mottle Mosaic Virus (CGMMV) in plants, in particular in plants susceptible to infection by CGMMV, such as Cucurbitaceae species, including melon, cucumber, watermelon and bottlegourd. The methods are based on the use of genetic constructs that induce post-transcriptional gene silencing and/or use a nucleotide sequence that encodes a defective variant of the replicase of CGMMV.

Abstract: DNA constructs comprising a first DNA segment that corresponds to at least a portion of a gene in the monolignol biosynthetic pathway, a spacer DNA segment, and a second DNA segment that is complementary to the first DNA segment can be used to reduce or modulate the lignin content in plants. In some embodiments, DNA constructs comprise at least a portion of a gene for 4CL, C3H, CCR, C4H or CCoAOMT. Vascular-preferred and constitutive promoters can be used to drive expression of the constructs.

Abstract: A method of increasing the content of one or more transgene-coded proteins or peptides in a plant is described. The increase is an effect of a decrease in the concentration of an ATP/ADP transporter in the plant. The method depends on transformation with and expression of a cDNA encoding an ATP/ADP transporter operably linked in antisense orientation to a promoter active in the plant.

Abstract: The present invention provides DNA and RNA designed on the basis of the sequence of an enzyme gene for repressing the expression of the enzyme gene generating a lachrymatory factor from a precursor of the lachrymatory factor, and also a vector for introducing DNA for repressing the expression of the gene of the lachrymatory factor-producing enzyme into a vegetable.

Abstract: Nucleic acid molecules are described encoding a starch granule-bound protein from rice as well as methods and recombinant DNA molecules for the production of transgenic plant cells and plants synthesizing a modified starch. Moreover, the plant cells and plants resulting from those methods as well as the starch obtainable therefrom are described.

Abstract: This invention provides the genes for indeterminate gametophyte (ig1), mutants of ig1, homologs of ig1, and orthologs of ig1, as well as the proteins encoded by these genes. This invention also provides compositions and methods which utilize these genes and proteins. Such methods include the creation of transgenic plants with antisense or expression constructs which comprise a nucleotide sequence derived from ig1.

Abstract: Rice sucrose synthase 3 regulatory regions suitable for directing expression of a heterologous nucleic acid are described, as well as nucleic acid constructs that include these regulatory regions. Also disclosed are transgenic plants that contain such constructs and methods of producing such transgenic plants.

Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.

Abstract: DNA encoding a plant quinolate phosphoribosyl transferase (QPRTase) enzyme, and constructs comprising such DNA are provided. Methods of altering quinolate phosphoribosyl transferase expression are provided.

Abstract: The present invention relates to compositions and methods for providing RNA Interference (RNAi) vectors comprising maize lignin biosynthesis enzymes for altering lignin content of plants. Specifically, plants comprising RNAi maize lignin vectors for reducing or altering lignin content are provided for reducing pretreatment costs of biofuel production. Additionally, RNAi maize lignin vectors are provided for altering cellulose production in plants for reducing pretreatment costs of plant biomass processing by increasing amounts of fermentable sugars.

Abstract: Compositions and methods for reducing the level of nornicotine and N?-nitrosonornicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.

Abstract: The present invention provides new miRNAs in rice. The nucleic acids of the invention can be used to control gene expression in plants.