Abstract: The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved storage stability. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions comprising neutral metalloprotease(s) obtained from Bacillus sp. In some more particularly preferred embodiments, the neutral metalloprotease is obtained from B. amyloliquefaciens. In still further preferred embodiments, the neutral metalloprotease is a variant of the B. amyloliquefaciens neutral metalloprotease. In yet additional embodiments, the neutral metalloprotease is a homolog of the the B. amyloliquefaciens neutral metalloprotease. The present invention finds particular use in applications including, but not limited to cleaning, bleaching and disinfecting.
Type:
Grant
Filed:
October 19, 2017
Date of Patent:
March 3, 2020
Assignee:
DANISCO US INC
Inventors:
Andrew Shaw, David A Estell, Eugene S Sadlowski, Hiroshi Oh, Louise Wallace, Ronaldus Wilhelmus Joannes Hommes, Sang-Kyu Lee
Abstract: The present invention relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present invention provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions, animal feed compositions and cleaning compositions. The invention also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
Type:
Grant
Filed:
March 28, 2018
Date of Patent:
February 18, 2020
Assignee:
Danisco US Inc.
Inventors:
Wolfgang Aehle, Piet Van Solingen, Martijn Scheffers, Richard Bott
Abstract: The present disclosure relates to serine proteases cloned from Bacillus gibsonii, and variants thereof. Compositions containing the serine proteases are suitable for use in cleaning fabrics and hard surfaces, as well as in a variety of industrial applications.
Type:
Grant
Filed:
December 12, 2014
Date of Patent:
January 14, 2020
Assignee:
DANISCO US INC
Inventors:
Marc Kolkman, Rie Mejldal, Frits Goedegebuur, Lilia Maria Babe, Anja Hemmingsen Kellett-Smith, Harm Mulder, Richard R. Bott, Miles Christopher Scotcher
Abstract: Disclosed are glycosyl hydrolase enzyme variants, particularly variants of certain oxidoreductases of glycosyl hydrolase family 61. Nucleic acids encoding the glycosyl hydrolyase variants, compositions including the glycosyl hydrolase variants, methods of producing the variants, and methods of using the variants are also described.
Type:
Grant
Filed:
July 24, 2014
Date of Patent:
November 19, 2019
Assignee:
DANISCO US INC
Inventors:
Nicholai Douglas, Maria Foukaraki, Ronaldus Wilhelmus Hommes, Thijs Kaper, Bradley R. Kelemen, Slavko Kralj, Suzanne E. Lantz, Jonathan K. Lassila, Igor Nikolaev, Daniel Esteban Torres-Pazmino, Wilhelmus Van Der Kley, Johannes Franciscus Thomas Van Lieshout, Sander Van Stigt Thans
Abstract: Disclosed are compositions and methods relating to variant maltohexaose-forming alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.
Type:
Grant
Filed:
May 20, 2016
Date of Patent:
October 29, 2019
Assignee:
DANISCO US INC
Inventors:
Luis G. Cascao-Pereira, David A. Estell, Marc Kolkman, Harm Mulder
Abstract: Described are methods for increasing the expression of a transgene in eukaryotic cells by reducing RNA interference (RNAi), and variant cells produce by the method. The methods and variant cells are useful, for example, for the efficient production of therapeutic and industrial polypeptides in eukaryotic cells.
Abstract: A recombinant yeast cell, fermentation compositions, and methods of use thereof are provided. The recombinant yeast cell includes at least one heterologous nucleic acid encoding one or more polypeptide having phosphoketolase activity; phosphotransacetylase activity; and/or acetylating acetaldehyde dehydrogenase activity, wherein the cell does not include a heterologous modified xylose reductase gene, and wherein the cell is capable of increased biochemical end product production in a fermentation process when compared to a parent yeast cell.
Abstract: Entomopathogenic fungal strains, compositions, and methods and compositions of producing and using the strains for reducing overall insect damage.
Type:
Application
Filed:
October 7, 2016
Publication date:
September 19, 2019
Applicant:
DANISCO US INC.
Inventors:
ANNE-SOPHIE MADELEINE ELISABETH ALDIGUIER, JACOB ANDREW LATONE, NICOLAS LEIVA, JOSHUA KLAUS MICHEL, ALEKSANDRA VIRAG
Abstract: The present invention provides a new fungal production system comprising a fungal host strain of Chrysosporium lucknowense wherein the endogenous cellulase secretion is less than 20% of the endogenous cellulase secretion of Chrysosporium lucknowense strain UV 18-25. Preferably, also the secretion of endogenous protease, endogenous ?-glucanase and endogenous cellobiohydrolase is less than 20% of the secretion of Chrysosporium lucknowense strain UV 18-25. Furthermore, fungal host strains are provided wherein several genes have been disrupted. According to another aspect of the invention a method for homologous and/or heterologous production of a pure protein with a purity of higher than 75%, comprising expressing a gene encoding said protein in the strains according to the invention have been described. Furthermore, a method for production of artificial protein mixes comprising expressing a gene encoding each of said proteins in a strain according to the invention have been disclosed.
Type:
Grant
Filed:
September 9, 2015
Date of Patent:
September 17, 2019
Assignee:
DANISCO US INC
Inventors:
Peter J. Punt, Richard Paul Burlingame, Christine M. Pynnonen, Phillip T. Olson, Jan Wery, Johannes Heinrich Visser, Mark A. Emalfarb, Jacob Visser, Jan Cornelis Verdoes
Abstract: The invention features compositions and methods for the increased production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in microorganisms via the heterologous expression of the mvaE and mvaS genes from the organisms Listeria grayi DSM 20601, Enterococcus faecium, Enterococcus gallinarum EG2, and Enterococcus casseliflavus.
Type:
Grant
Filed:
September 1, 2017
Date of Patent:
July 30, 2019
Assignees:
Danisco US Inc., The Goodyear Tire & Rubber Company
Inventors:
Zachary Q. Beck, Michael C. Miller, Caroline M. Peres, Yuliya A. Primak, Jeff P. Pucci, Derek H. Wells
Abstract: The invention provides compositions and methods for clostridial bacteria that have been engineered to produce and/or to improve efficiency of production of industrial bioproducts.
Abstract: The present invention provides lipolytic enzyme variants. Specifically, the present invention provides lipolytic enzyme variants having two, three, or more modifications as compared to a parent lipolytic enzyme and having at least one improved property. In addition, the present invention provides compositions comprising a lipolytic enzyme variant of the invention. The present invention also provides methods of cleaning using compositions comprising a lipolytic enzyme variant of the invention.
Type:
Grant
Filed:
October 24, 2017
Date of Patent:
June 25, 2019
Assignee:
DANISCO US INC
Inventors:
Thomas P. Graycar, Ayrookaran J. Poulose, David A. Estell
Abstract: The present disclosure relates to polypeptides having alpha-glucosidase activity isolated, derived or derivable from Rasamsonia or engineered polypeptides having alpha-glucosidase activity isolated, derived or derivable from Rasamsonia homologs. The present disclosure also pertains to polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, host cells and mutant cells comprising the polynucleotides. The disclosure further pertains to compositions comprising such polypeptides, methods of producing the polypeptides and compositions, as well as methods for using such polypeptides and compositions for industrial applications.
Abstract: The present invention relates in general to bacterial cells having a genetic alteration that results in increased expression of a protein of interest and methods of making and using such cells. Aspects of the present invention include Gram positive microorganisms, such as Bacillus species, having a genetic alteration that modifies activity of a protein encoded by the ykf operon and results in enhanced expression of a protein of interest.
Type:
Grant
Filed:
December 8, 2014
Date of Patent:
May 14, 2019
Assignee:
DANISCO US INC
Inventors:
Cristina Bongiorni, Rei Otsuka, Brian F. Schmidt, Anita Van Kimmenade
Abstract: The present disclosure is generally directed to glycosyl hydrolase enzymes, compositions comprising such enzymes, and methods of using the enzymes and compositions, for example for the saccharification of cellulosic and hemicellulosic materials into sugars.
Type:
Grant
Filed:
July 27, 2015
Date of Patent:
April 30, 2019
Assignee:
DANISCO US INC.
Inventors:
Benjamin Bower, Megan Yee Hsi, Thijs Kaper, Bradley R. Kelemen, Suzanne E. Lantz, Edmund A. Larenas, Colin Mitchinson, Steven Kim, William D. Hitz, Mark Emptage, Keith Dumont Wing
Abstract: Described are compositions and methods relating variant filamentous fungi having altered growth characteristics. Such variants are well-suited for growth in submerged cultures, e.g., for the large-scale production of enzymes and other proteins for commercial applications.
Type:
Grant
Filed:
August 25, 2011
Date of Patent:
April 23, 2019
Assignee:
DANISCO US INC
Inventors:
Timothy C. Dodge, Aleksandra Virag, Michael Ward
Abstract: This present invention relates to cultured recombinant cells comprising a heterologous phosphoketolase (PKL) polypeptide that are capable of increased production of acetyl coenzyme A-derived metabolites, as well as methods for producing and using the same. In some embodiments, the recombinant cells further comprise one or more mevalonate (MVA) pathway polypeptides for the production of isoprenoid precursors, isoprene and isoprenoids.
Type:
Grant
Filed:
April 10, 2014
Date of Patent:
April 2, 2019
Assignees:
DANISCO US INC., THE GOODYEAR TIRE & RUBBER COMPANY
Inventors:
Zachary Q. Beck, Jeffrey W. Munos, Derek H. Wells, Jian Yao
Abstract: A recombinant yeast cell, fermentation compositions, and methods of use thereof are provided. The recombinant yeast cell includes at least one heterologous nucleic acid encoding one or more polypeptide having phosphoketolase activity; phosphotransacetylase activity; and/or acetylating acetaldehyde dehydrogenase activity, wherein the cell does not include a heterologous modified xylose reductase gene, and wherein the cell is capable of increased biochemical end product production in a fermentation process when compared to a parent yeast cell.