Patents Assigned to Danisco US Inc.
  • Compositions and methods comprising cellulase variants with reduced affinity to non-cellulosic materials
    Patent number: 9695407
    Abstract: The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having reduced binding to non-cellulosic materials. Also described are nucleic acids encoding the cellulase, compositions comprising said cellulase, methods of identifying cellulose variants and methods of using the compositions.
    Type: Grant
    Filed: March 19, 2015
    Date of Patent: July 4, 2017
    Assignee: DANISCO US INC
    Inventors: Luis Cascao-Pereira, Thijs Kaper, Bradley R. Kelemen, Amy D. Liu
  • SIMULTANEOUS SACCHARIFICATION AND CO-FERMENTATION OF GLUCOAMYLASE-EXPRESSING FUNGAL STRAINS WITH AN ETHANOLOGEN TO PRODUCE ALCOHOL FROM CORN
    Publication number: 20170183691
    Abstract: A conversion process provides using different co-cultured cell lines to express different sets of enzymes catalyzing the same process. For example, in a Simultaneous Saccharification and Co-Fermentation (SSCF) process, a starch substrate is converted to alcohol by contacting the substrate with yeast and Aspergillus niger cells. Because A. niger expresses an endogenous glucoamylase and alpha-amylase, these enzymes do not need to be added during the SSCF process.
    Type: Application
    Filed: April 6, 2015
    Publication date: June 29, 2017
    Applicant: Danisco US Inc.
    Inventors: Kathleen A. Clarkson, Matthew T. Reboli, Jacquelyn A. Huitink, Paula Johanna Maria Teunissen, Gopal K. Chotani, Jayarama K. Shetty
  • RECOMBINANT MICROORGANISMS FOR THE ENHANCED PRODUCTION OF MEVALONATE, ISOPRENE, ISOPRENOID PRECURSORS, ISOPRENOIDS, AND ACETYL-COA-DERIVED PRODUCTS
    Publication number: 20170159074
    Abstract: The invention features compositions and methods for the increased production of mevalonate, isoprene, isoprenoid precursor molecules, isoprenoids, and/or acetyl-CoA-derived products in recombinant microorganisms by engineering the microorganisms to comprise one or more acetylating proteins such that the expression and/or activity of the one or more acetylating proteins is modulated.
    Type: Application
    Filed: February 20, 2015
    Publication date: June 8, 2017
    Applicant: Danisco US Inc.
    Inventors: Marguerite A. CERVIN, Michael C. MILLER, Derek H. WELLS, Alan J. WOLFE, Madhukar S. DASIKA
  • BUTTIAUXELLA SP. PHYTASE VARIANTS
    Publication number: 20170143004
    Abstract: Provided herein are variants of Buttiauxella sp. phytases that may be used in industrial applications including methods for starch liquefaction, alcohol fermentations and for enhancing phosphate digestion in foods and animal feeds.
    Type: Application
    Filed: July 25, 2016
    Publication date: May 25, 2017
    Applicant: Danisco US Inc.
    Inventors: Oliver Kensch, Klaus Schulze Pellengahr, Birgitta Leuthner, Jayarama K. Shetty, Michael Pepsin, Soren Dalsgaard, Mai Faurschou Isaksen
  • BETA-GLUCOSIDASE I VARIANTS WITH IMPROVED PROPERTIES
    Publication number: 20170145396
    Abstract: The present disclosure is generally directed to enzymes and in particular beta-glucosidase variants. Also described are nucleic acids encoding beta-glucosidase variants, compositions comprising beta-glucosidase variants, methods of using beta-glucosidase variants, and methods of identifying additional useful beta-glucosidase variants.
    Type: Application
    Filed: August 29, 2016
    Publication date: May 25, 2017
    Applicant: DANISCO US INC.
    Inventors: Richard R. BOTT, Thijs KAPER, Bradley R. KELEMEN, Frits GOEDEGEBUUR, Ronaldus Wilhelmus Joannes HOMMES, Slavko KRALJ, Paulien KRUITHOF, Igor NIKOLAEV, Wilhelmus Antonious Hendricus VAN DER KLEY, Johannes Franciscus Thomas VAN LIESHOUT, Sander VAN STIGT THANS, Gudrun VOGTENTANZ, Mats SANDGREN
  • Small enzyme-containing granules
    Patent number: 9657284
    Abstract: The invention provides small enzyme-containing granules having an inorganic salt core and an enzyme-containing layer coated over the core, and methods for producing such granules. The majority of the enzyme granules are less than 300 ?m in diameter. The granules are suitable for incorporation into compositions such as cleaning, textile processing, and animal feed compositions.
    Type: Grant
    Filed: April 6, 2015
    Date of Patent: May 23, 2017
    Assignee: DANISCO US INC.
    Inventors: Nathaniel T. Becker, Robert Christensen, Mark Gebert, Pekka Vaha-VaHe
  • Variant CBH1.1
    Patent number: 9644192
    Abstract: Disclosed are variants of Humicola grisea CeI7A (CBH1.1), H. jecorina CBH1 variant or S. thermophilium CBH1, nucleic acids encoding the same and methods for producing the same. The variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted.
    Type: Grant
    Filed: August 20, 2014
    Date of Patent: May 9, 2017
    Assignee: Danisco US Inc., Genencor Division
    Inventors: Frits Goedegebuur, Peter Gualfetti, Colin Mitchinson, Edmund Larenas
  • Glucoamylase Variants with Altered Properties
    Publication number: 20170114336
    Abstract: The present invention relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present invention provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions, animal feed compositions and cleaning compositions. The invention also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
    Type: Application
    Filed: August 5, 2016
    Publication date: April 27, 2017
    Applicant: Danisco US Inc.
    Inventors: Wolfgang Aehle, Piet Van Solingen, Martijn Scheffers, Richard Bott
  • METHOD FOR IMPROVING YIELD OF CELLULOSE CONVERSION PROCESSES
    Publication number: 20170107548
    Abstract: The present teachings provide methods of converting cellulosic materials to soluble sugars. Methods for increasing the yield of glucose from the enzymatic saccharification of cellulosic materials is also provided. The present teachings further provide methods of increasing the yield of cellobiose from the enzymatic saccharification of cellulosic materials.
    Type: Application
    Filed: September 9, 2016
    Publication date: April 20, 2017
    Applicant: DANISCO US INC.
    Inventors: Bradley R. KELEMEN, Edmund A. LARENAS, Colin MITCHINSON
  • Enhanced protein expression in
    Patent number: 9617549
    Abstract: The present invention provides cells that have been genetically manipulated to have an altered capacity to produce expressed proteins. In particular, the present invention relates to Gram-positive microorganisms, such as Bacillus species having enhanced expression of a protein of interest, wherein one or more chromosomal genes have been inactivated, and preferably wherein one or more chromosomal genes have been deleted from the Bacillus chromosome. In some further embodiments, one or more indigenous chromosomal regions have been deleted from a corresponding wild-type Bacillus host chromosome.
    Type: Grant
    Filed: September 17, 2015
    Date of Patent: April 11, 2017
    Assignee: Danisco US Inc.
    Inventors: Eugenio Ferrari, Carole Harbison, M. Harunur Rashid, Walter Weyler
  • SERINE PROTEASES OF BACILLUS SPECIES
    Publication number: 20170096653
    Abstract: The present disclosure relates to serine proteases cloned from Bacillus spp., and variants thereof. Compositions containing the serine proteases are suitable for use in cleaning fabrics and hard surfaces, as well as in a variety of industrial applications.
    Type: Application
    Filed: March 20, 2015
    Publication date: April 6, 2017
    Applicant: DANISCO US INC.
    Inventors: Marc KOLKMAN, Anja HEMMINGSEN KELLETT-SMITH, Rie MEJLDAL, Richard R. BOTT, Lilia Maria BABE
  • METHOD FOR REDUCING VISCOSITY IN SACCHARIFICATION PROCESS
    Publication number: 20170096651
    Abstract: The present invention relates to compositions that can be used in hydrolyzing biomass such as compositions comprising a polypeptide having glycosyl hydrolase family 61/endoglucanase activity, methods for hydrolyzing biomass material, and methods for reducing viscosity of biomass mixture using a composition comprising a polypeptide having glycosyl hydrolase family 61/endoglucanase activity.
    Type: Application
    Filed: August 6, 2015
    Publication date: April 6, 2017
    Applicant: DANISCO US INC.
    Inventors: Colin MITCHINSON, Mian LI, Bradley R. KELEMEN, Suzanne E. LANTZ, Keith D. WING, William D. HITZ
  • ALTERED HOST CELL PATHWAY FOR IMPROVED ETHANOL PRODUCTION
    Publication number: 20170088861
    Abstract: A recombinant yeast cell, fermentation compositions, and methods of use thereof are provided. The recombinant yeast cell includes at least one heterologous nucleic acid encoding one or more polypeptide having phosphoketolase activity; phosphotransacetylase activity; and/or acetylating acetaldehyde dehydrogenase activity, wherein the cell does not include a heterologous modified xylose reductase gene, and wherein the cell is capable of increased biochemical end product production in a fermentation process when compared to a parent yeast cell.
    Type: Application
    Filed: March 19, 2015
    Publication date: March 30, 2017
    Applicant: Danisco US Inc.
    Inventors: Miasnikov Andrei, Jeffrey W. Munos
  • Filamentous fungi having an altered viscosity phenotype
    Patent number: 9593341
    Abstract: Described are compositions and methods relating to variant filamentous fungi having altered growth characteristics. Such variants are well-suited for growth in submerged cultures, e.g., for the large-scale production of enzymes and other proteins for commercial applications.
    Type: Grant
    Filed: April 20, 2012
    Date of Patent: March 14, 2017
    Assignee: DANISCO US INC.
    Inventors: Elizabeth A. Bodie, Robert James Pratt, II
  • Proteases with modified pro regions
    Patent number: 9593320
    Abstract: The present invention provides methods and compositions for the production of mature proteases in bacterial host cells. The compositions include modified polynucleotides that encode modified proteases, which have at least one mutation in the pro region; the modified serine proteases encoded by the modified polynucleotides; expression cassettes, DNA constructs, and vectors comprising the modified polynucleotides that encode the modified proteases; and the bacterial host cells transformed with the vectors of the invention. The methods include methods for enhancing the production of mature proteases in bacterial host cells e.g. Bacillus sp. host cells. The produced proteases find use in the industrial production of enzymes, suitable for use in various industries, including but not limited to the cleaning, animal feed and textile processing industry.
    Type: Grant
    Filed: July 31, 2015
    Date of Patent: March 14, 2017
    Assignee: DANISCO US INC.
    Inventors: Eugenio Ferrari, Carol Fioresi, Anita van Kimmenade
  • Filamentous fungi having an altered viscosity phenotype
    Patent number: 9587242
    Abstract: Described are compositions and methods relating to variant filamentous fungi having altered growth characteristics. Such variants are well-suited for growth in submerged cultures, e.g., for the large-scale production of enzymes and other proteins for commercial applications.
    Type: Grant
    Filed: April 20, 2012
    Date of Patent: March 7, 2017
    Assignee: DANISCO US INC.
    Inventors: Elizabeth A. Bodie, Robert James Pratt, II
  • ALPHA AMYLASE VARIANTS DERIVED FROM THE ALPHA AMYLASE OF CYTOPHAGA SP. AMYLASE (CSPAMY2)
    Publication number: 20170037387
    Abstract: Disclosed are compositions and methods relating to variant alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.
    Type: Application
    Filed: October 7, 2016
    Publication date: February 9, 2017
    Applicant: Danisco US Inc.
    Inventors: Luis G. Cascao-Pereira, David A. Estell, Marc Kolkman
  • USE AND PRODUCTION OF STORAGE-STABLE NEUTRAL METALLOPROTEASE
    Publication number: 20170022490
    Abstract: The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved storage stability. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions comprising neutral metalloprotease(s) obtained from Bacillus sp. In some more particularly preferred embodiments, the neutral metalloprotease is obtained from B. amyloliquefaciens. In still further preferred embodiments, the neutral metalloprotease is a variant of the B. amyloliquefaciens neutral metalloprotease. In yet additional embodiments, the neutral metalloprotease is a homolog of the the B. amyloliquefaciens neutral metalloprotease. The present invention finds particular use in applications including, but not limited to cleaning, bleaching and disinfecting.
    Type: Application
    Filed: April 8, 2016
    Publication date: January 26, 2017
    Applicant: Danisco US Inc.
    Inventors: Andrew Shaw, Louise Wallace, David A. Estell, Ronaldus W.J. Hommes, Sang-Kyu Lee, Hiroshi Oh, Eugene S. Sadlowski
  • Variant Maltohexaose-Forming Alpha-Amylase Variants
    Publication number: 20170009219
    Abstract: Disclosed are compositions and methods relating to variant maltohexaose-forming alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.
    Type: Application
    Filed: May 20, 2016
    Publication date: January 12, 2017
    Applicant: Danisco US Inc.
    Inventors: Luis G. Cascao-Pereira, David A. Estell, Marc Kolkman, Harm Mulder
  • STRATEGY FOR SUCROSE REDUCTION AND GENERATION OF INSOLUBLE FIBER IN JUICES
    Publication number: 20170006902
    Abstract: The present teachings provide a method of making a lower calorie, higher insoluble fiber beverage comprising; treating a sucrose-containing beverage with a glucosyltransferase to convert sucrose to alpha (1-3) glucan to make the lower calorie, higher insoluble fiber beverage. Additional methods, as well as compositions, are provided.
    Type: Application
    Filed: February 4, 2015
    Publication date: January 12, 2017
    Applicant: Danisco US Inc.
    Inventor: Adam L. Garske