Abstract: The present invention provides serine protease variants, more specifically subtilisin variants produced there from. Specifically, the present invention provides serine protease variants, more specifically subtilisin variants having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides compositions comprising these serine protease variants, more specifically subtilisin variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these serine protease variants, more specifically subtilisin variants.
Type:
Grant
Filed:
May 4, 2012
Date of Patent:
January 2, 2018
Assignee:
DANISCO US INC.
Inventors:
Neelam S. Amin, Katherine Augustyn, Joshua R. Basler, Luis G. Cascao-Pereira, Katherine D. Collier, Edward M. Concar, David A. Estell, James T. Kellis, Jr., Euan John Magennis, Alexander Pisarchik, Ayrookaran J. Poulose, Philip Frank Souter, Glenn Steven Ward, Jian Yao
Abstract: The present invention relates in general to bacterial cells having a genetic alteration that results in increased expression of a protein of interest and methods of making and using such cells. Aspects of the present invention include Grampositive microorganisms, such as members of the Bacillus genus having a genetic alteration that delays, reduces, or blocks the expression or activation of genes for sporulation, thereby resulting in enhanced expression of a protein of interest. The genetic alteration is one that reduces expression of a kinA gene, a phrA gene or a phrE gene.
Type:
Application
Filed:
December 11, 2015
Publication date:
December 28, 2017
Applicant:
DANISCO US INC.
Inventors:
Cristina BONGIORNI, Robert I. CHRISTENSEN, Brian F. SCHMIDT, Anita VAN KIMMENADE
Abstract: The present invention relates in general to bacterial cells having genetic alterations that result in increased expression of a protein of interest and methods of making and using such cells. Aspects of the present invention include altered Gram positive microorganismshaving one or more a genetic alterations that reduce the expression of a gene in the sin operon, thereby resulting in the enhanced expression of one or more proteins of interest.
Type:
Application
Filed:
December 3, 2015
Publication date:
December 21, 2017
Applicant:
DANISCO US INC.
Inventors:
Cristina BONGIORNI, Rodante GONZALES CAGUIAT, Carol Marie FIORESI, Brian F. SCHMIDT, Anita VAN KIMMENADE, Chao ZHU
Abstract: Described are compositions and methods relating to the thermostable fungal cellulase enzyme, EGV, and Trichoderma host cells having a modification comprising or consisting essentially of disruption or deletion of nucleotide(s) for expression of this cellulose, whereby EGV expression is prevented.
Type:
Grant
Filed:
August 20, 2015
Date of Patent:
November 28, 2017
Assignee:
DANISCO US INC
Inventors:
Andrei Miasnikov, Michael Schelle, Michael Ward
Abstract: Hybrid alpha-amylases are provided that share a conserved 3D structure in whole or in part with a wild-type Termamyl-like ?-amylase, e.g., a Bacillus amylase. In the hybrid, an N-terminal portion of a Termamyl-like ?-amylase is replaced with sequences from an archae ?-amylase. The sequence similarity between the two amylase sequences may be less than 60%. Conserving the wild-type 3D structure in the hybrid facilitates obtaining enzymatically active amylases. In one embodiment, one or both amylase sequences contribute residues to the B domain, resulting in particularly advantageous properties. For instance, replacement of the Ca2+ binding site in the B domain of the Termamyl-like ?-amylase with a B domain sequence of an archae ?-amylase that does not bind Ca2+ can produce a hybrid that is fully active in the absence of Ca2+.
Abstract: The present invention provides a method for producing an alcohol comprising: (a) admixing a tripeptidyl peptidase, predominantly having exopeptidase activity, with a feedstock or a fraction thereof before, during or after fermentation of said feedstock or a fraction thereof; and (b) recovering an alcohol. Also provided are uses of a tripeptidyl peptidase and by-products of alcohol production obtainable by the method of the invention.
Type:
Application
Filed:
October 23, 2015
Publication date:
October 26, 2017
Applicant:
DANISCO US INC.
Inventors:
Andrei MIASNIKOV, Maria MA, Scott D. POWER
Abstract: The invention provides small enzyme-containing granules having an inorganic salt core and an enzyme-containing layer coated over the core, and methods for producing such granules. The majority of the enzyme granules are less than 300 ?m in diameter. The granules are suitable for incorporation into compositions such as cleaning, textile processing, and animal feed compositions.
Type:
Application
Filed:
April 18, 2017
Publication date:
October 5, 2017
Applicant:
DANISCO US INC.
Inventors:
Nathaniel T. BECKER, Robert I. CHRISTENSEN, Mark S. GEBERT, Antti Pekka VAHA-VAHE
Abstract: Methods for obtaining corn oil from milled corn germ (e.g., dry milled corn germ), involving adding water, at least one acidic cellulase, at least one acidic protease, and at least one phytase to milled corn germ to obtain corn oil.
Type:
Grant
Filed:
January 13, 2015
Date of Patent:
October 3, 2017
Assignees:
The United States of America, as Represented by the Secretary of Agriculture, DANISCO US INC.
Inventors:
Robert A. Moreau, David Johnston, Kevin B. Hicks, Jayarama K. Shetty
Abstract: The invention features methods for producing isoprene from cultured cells wherein the cells in the stationary phase. The invention also provides compositions that include these cultured cells and/or increased amount of isoprene. The invention also provides for systems that include a non-flammable concentration of isoprene in the gas phase. Additionally, the invention provides isoprene compositions, such as compositions with increased amount of isoprene or increased purity.
Type:
Grant
Filed:
December 17, 2015
Date of Patent:
October 3, 2017
Assignees:
Danisco US Inc., The Goodyear Tire & Rubber Company
Inventors:
Anthony R. Calabria, Marguerite A. Cervin, Gopal K. Chotani, Richard La Duca, Joseph C. McAuliffe, Michael C. Miller, Timothy A. Sabo, Karl J. Sanford, Erin L. Spring, Gregory M. Whited
Abstract: Bacillus agaradhaerens strain WDG185 expresses an inulosucrase that efficiently synthesizes a broad range of IOS with a GF range of GF3-GF30. The isolated and/or purified inulosucrase, recombinantly engineered variants thereof, active fragments thereof, synthetic nucleic acids encoding the inulosucrase, its variants, or its active fragments, host cells comprising the synthetic nucleic acids, and compositions comprising the inulosucrase are provided. Methods of using the compositions include the manufacture of inulooligosaccharides.
Type:
Application
Filed:
December 4, 2015
Publication date:
September 21, 2017
Applicant:
DANISCO US INC.
Inventors:
Slavko KRALJ, Marc KOLKMAN, Chris LEEFLANG, Johannes G. DE NOBEL, Arjen HOEKSTRA, Veil ALKAN
Abstract: A fungal alpha-amylase is provided from Aspergillus fumigatus (AfAmyl). AfAmyl has an optimal pH of 3.5 and is operable at 30-75 degrees C., allowing the enzyme to be used in combination with a glucoamylase and an isoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AfAmyl also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DPI+DP2) compared to the products of saccharification catalyzed by an alpha-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.
Type:
Grant
Filed:
December 5, 2013
Date of Patent:
September 19, 2017
Assignee:
DANISCO US INC
Inventors:
Ling Hua, Martijn Scheffers, Marco Van Brussel, Casper Vroemen, Bo Zhang, Zhengong Zhang
Abstract: Provided are fungal host strains and recombinant DNA constructs for creation and use thereof, wherein the fungal host strains are particularly stable and useful for expressing proteins, enzymes, variants and other substances of interest in a reliable or less variable fashion.
Type:
Application
Filed:
December 1, 2015
Publication date:
September 14, 2017
Applicant:
DANISCO US INC.
Inventors:
Mark ARENTSHORST, Sharief BARENDS, Paulien KRUITHOF, Igor NIKOLAEV, Jean Paul OUEDRAOGO, Arthur F.J. RAM
Abstract: Methods and apparatus for the purification of isoprene, such as the purification of a bioisoprene composition from fermentor off-gas. The apparatus includes two columns that process the fermentor off-gas, which includes isoprene and various impurities. A solvent is added to the off-gas in the first column, and the isoprene is stripped from the solvent in the second column. Also provided is a downstream further purification process. Also provided are the resulting purified isoprene compositions.
Type:
Grant
Filed:
April 9, 2015
Date of Patent:
September 5, 2017
Assignees:
Danisco US Inc., The Goodyear Tire & Rubber Company
Inventors:
Frank J. Feher, John Kaluen Kan, Joseph C. McAuliffe, Thomas F. McCall, Lawrence J. Pickert, Christopher D. Ploetz, Stephan Rodewald, Timothy A. Sabo, Tang H. Wong
Abstract: The invention features compositions and methods for the increased production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in microorganisms via the heterologous expression of the mvaE and mvaS genes from the organisms Listeria grayi DSM 20601, Enterococcus faecium, Enterococcus gallinarum EG2, and Enterococcus casseliflavus.
Type:
Grant
Filed:
October 15, 2014
Date of Patent:
September 5, 2017
Assignees:
Danisco US Inc., The Goodyear Tire & Rubber Company
Inventors:
Zachary Q. Beck, Michael C. Miller, Caroline M. Peres, Yuliya A. Primak, Jeff P. Pucci, Derek H. Wells
Abstract: The invention features methods producing isoprene and a co-product, such as ethanol, 1,3-propanediol, or hydrogen from cultured cells. The invention also provides compositions that include these cultured cells. The invention provides compositions comprising isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen. Additionally, the invention provides methods of co-producing isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen by culturing cells under conditions suitable for co-production of isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen.
Type:
Grant
Filed:
January 6, 2014
Date of Patent:
September 5, 2017
Assignees:
Danisco US Inc., The Goodyear Tire & Rubber Company
Inventors:
Gopal K. Chotani, Joseph C. McAuliffe, Caroline M. Peres, Karl J. Sanford, Derek H. Wells
Abstract: Aspects of the present disclosure are drawn to methods of improving the expression of secreted cuproenzymes from host cells by manipulating the expression level of one or more proteins involved in copper transport in the host cell, e.g., membrane-bound copper transporting ATPases and soluble copper transporters. The present disclosure also provides compositions containing such improved host cells as well as products derived from the improved host cells that contain one or more cuproenzymes of interest.
Abstract: Described are compositions and methods relating to variant filamentous fungi having altered growth characteristics. Such variants are well-suited for growth in submerged cultures, e.g., for the large-scale production of enzymes and other proteins for commercial applications.
Type:
Grant
Filed:
April 20, 2012
Date of Patent:
August 8, 2017
Assignee:
DANISCO US INC
Inventors:
Elizabeth A. Bodie, Robert James Pratt, II
Abstract: Described are compositions and methods relating to variant filamentous fungi having altered growth characteristics. Such variants are well-suited for growth in submerged cultures, e.g., for the large-scale production of enzymes and other proteins for commercial applications.
Type:
Grant
Filed:
April 20, 2012
Date of Patent:
August 8, 2017
Assignee:
DANISCO US INC
Inventors:
Elizabeth A. Bodie, Robert James Pratt, II
Abstract: The present compositions and methods relate to a beta-mannanase from Paenibacillus macerans, polynucleotides encoding the beta-mannanase, and methods of make and/or use thereof. Formulations containing the beta-mannanase are suitable for use in hydrolyzing lignocellulosic biomass substrates, especially those comprising a measurable level of galactoglucomannan (GGM) and/or glucomannan (GM).
Abstract: The present disclosure relates to filamentous fungal host strains and recombinant DNA constructs for creation and use thereof. The filamentous fungal host strains are particularly useful for efficiently screening DNA libraries encoding recombinant proteins.
Type:
Grant
Filed:
June 3, 2011
Date of Patent:
July 11, 2017
Assignee:
DANISCO US INC.
Inventors:
Benjamin S. Bower, Thijs Kaper, Bradley R. Kelemen