Abstract: The present invention relates in general to bacterial cells having a genetic alteration that results in increased expression of a protein of interest and methods of making and using such cells. Aspects of the present invention include Gram positive microorganisms, such as Bacillus species, having a genetic alteration that reduces the expression of a gene in the pdh operon and results in enhanced expression of a protein of interest.
Type:
Application
Filed:
December 5, 2014
Publication date:
January 31, 2019
Applicant:
DANISCO US INC.
Inventors:
Cristina BONGIORNI, Brian F. SCHMIDT, Anita VAN KIMMENADE
Abstract: The present invention relates to compositions that can be used in hydrolyzing biomass such as compositions comprising a polypeptide having glycosyl hydrolase family 61/endoglucanase activity, methods for hydrolyzing biomass material, and methods for reducing viscosity of biomass mixture using a composition comprising a polypeptide having glycosyl hydrolase family 61/endoglucanase activity.
Type:
Grant
Filed:
August 6, 2015
Date of Patent:
January 29, 2019
Assignee:
DANISCO US INC.
Inventors:
Colin Mitchinson, Mian Li, Bradley R. Kelemen, Suzanne E. Lantz, Keith D. Wing, William D. Hitz
Abstract: The present invention relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present invention provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions, animal feed compositions and cleaning compositions. The invention also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
Type:
Application
Filed:
March 28, 2018
Publication date:
January 3, 2019
Applicant:
Danisco US Inc.
Inventors:
Wolfgang Aehle, Piet Van Solingen, Martijn Scheffers, Richard Bott
Abstract: Disclosed are glycosyl hydrolase enzyme variants, particularly variants of certain oxidoreductases of glycosyl hydrolase family 61. Nucleic acids encoding the glycosyl hydrolyase variants, compositions including the glycosyl hydrolase variants, methods of producing the variants, and methods of using the variants are also described.
Type:
Grant
Filed:
July 24, 2014
Date of Patent:
January 1, 2019
Assignee:
DANISCO US INC
Inventors:
Maria Foukaraki, Ronaldus Wilhelmus Hommes, Thijs Kaper, Bradley R. Kelemen, Slavko Kralj, Suzanne E. Lantz, Igor Nikolaev, Wilhelmus Van Der Kley, Johan Franciscus Thomas Van Lieshout, Sander Van Stigt Thans
Abstract: The present disclosure is generally related to modified Gram-positive bacterial cells producing increased amounts of one or more protein(s) of interest. Certain embodiments of the instant disclosure are therefore directed to modified Gram-positive bacterial cells expressing an increased amount of a POI relative to unmodified (i.e., parental) Gram-positive bacterial cells, wherein the modified (i.e., daughter) bacterial cells comprise a modification which increases rasP gene expression. In certain other embodiments, the disclosure pertains to methods of modifying bacterial cells such that the modified (daughter) cells produce an increased level of a protein of interest. In other embodiments, the disclosure pertains to a protein of interest produced by fermenting a modified bacterial cell of the instant disclosure. Certain other embodiments of the disclosure are directed to one or more proteinaceous compositions comprising one or more protein(s) of interest thus made.
Type:
Application
Filed:
December 21, 2016
Publication date:
December 27, 2018
Applicant:
Danisco US Inc.
Inventors:
Christina Bongiorni, Jolanda Neef, Brian F. Schmidt, Anita Van Kimmenade, Jan Maarten Van Dijl
Abstract: The present disclosure is directed, in a first aspect, to the use of inverting beta-xylosidase enzymes to reduce byproduct formation and increase the yield of fermentation products, as well as, in a second aspect, to the use of retaining beta-xylosidase enzymes to improve production of alkyl-beta-xylopyranoside compounds, in a simultaneous saccharification and fermentation reactions.
Type:
Grant
Filed:
December 17, 2010
Date of Patent:
November 27, 2018
Assignee:
DANISCO US INC.
Inventors:
Christina Gutierrez, Colin Mitchinson, Tom T. Huang, Bruce A. Diner, Paul Joseph Fagan, William D. Hitz
Abstract: The invention features compositions and methods for the increased production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in microorganisms by engineering a microorganism for increased carbon flux towards mevalonate production in the following enzymatic pathways: (a) citrate synthase, (b) phosphotransacetylase, (c) acetate kinase, (d) lactate dehydrogenase, (e) malic enzyme, and (f) pyruvate dehydrogenase such that one of more of the enzyme activity is modulated. In addition, production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids can be further enhanced by the heterologous expression of the mvaE and mvaS genes (such as, but not limited to, mvaE and mvaS genes from the organisms Listeria grayi DSM 20601, Enterococcus faecium, Enterococcus gallinarum EG2, and Enterococcus casseliflavus).
Type:
Grant
Filed:
July 22, 2015
Date of Patent:
November 27, 2018
Assignees:
Danisco US Inc., The Goodyear Tire & Rubber Company
Inventors:
Zachary Q. Beck, Michael C. Miller, Caroline M. Peres, Yuliya A. Primak, Jeff P. Pucci, Derek H. Wells
Abstract: The invention provides for methods for the production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in cells via the heterologous expression of phosphoketolase enzymes.
Type:
Grant
Filed:
September 28, 2016
Date of Patent:
October 30, 2018
Assignees:
Danisco US Inc., The Goodyear Tire & Rubber Company
Inventors:
Zachary Q. Beck, Caroline M. Peres, Dmitrii V. Vaviline, Andrew C. Eliot
Abstract: The present invention provides methods for protein engineering and serine protease variants produced there from. Specifically, the present invention provides serine protease variants having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides compositions comprising these serine protease variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these serine protease variants.
Type:
Grant
Filed:
July 21, 2014
Date of Patent:
October 9, 2018
Assignee:
DANISCO US INC.
Inventors:
Wolfgang Aehle, Luis G. Cascao-Pereira, David A. Estell, Frits Goedegebuur, James T. Kellis, Jr., Ayrookaran J. Poulose, Brian F. Schmidt
Abstract: The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.
Type:
Application
Filed:
January 17, 2018
Publication date:
September 27, 2018
Applicants:
Danisco US Inc., The Goodyear Tire & Rubber Company
Inventors:
Marguerite A. CERVIN, Gopal K. CHOTANI, Frank J. FEHER, Richard LA DUCA, Joseph C. MCAULIFFE, Andrei MIASNIKOV, Caroline M. PERES, Aaron S. PUHALA, Karl J. SANFORD, Fernando VALLE, Gregory M. WHITED
Abstract: Disclosed are glycosyl hydrolase enzyme variants, particularly variants of certain oxidoreductases of glycosyl hydrolase family 61. Nucleic acids encoding the glycosyl hydrolyase variants, compositions including the glycosyl hydrolase variants, methods of producing the variants, and methods of using the variants are also described.
Type:
Grant
Filed:
July 24, 2014
Date of Patent:
September 25, 2018
Assignee:
DANISCO US INC.
Inventors:
Maria Foukaraki, Ronaldus Wilhelmus Hommes, Thijs Kaper, Bradley R. Kelemen, Slavko Kralj, Suzanne E. Lantz, Igor Nikolaev, Wilhelmus Van Der Kley, Johannes Franciscus Thomas Van Lieshout, Sander Van Stigt Thans
Abstract: Provided are cells having an increased protein production characterized in that said cell comprises modified SUMOylation, a process for producing such a cell or expression system and the use of such a cell in producing a protein of interest.
Type:
Grant
Filed:
February 22, 2017
Date of Patent:
September 11, 2018
Assignee:
DANISCO US INC
Inventors:
Jurian Alexander Bronkhof, Jean-Paul Meijnen, Carola Bianca Michielse, Ismail Atalay Van Der Burgt, Jacob Visser, Johannes Heinrich Visser
Abstract: The invention provides a culture plate made from a polymer incorporating a culture component releasable into culture media in the well, methods of culturing a microorganisms in the culture plate, and a methods of making the culture plate.
Type:
Grant
Filed:
September 20, 2013
Date of Patent:
July 24, 2018
Assignee:
DANISCO US INC.
Inventors:
Jimmy Chan, Rachel E. Muir, William Throndset
Abstract: The present invention provides lipolytic enzyme variants. Specifically, the present invention provides lipolytic enzyme variants having one or more modifications as compared to a parent lipolytic enzyme having at least one improved property. In addition, the present invention provides compositions comprising a lipolytic enzyme variant of the invention. The present invention also provides methods of cleaning using compositions comprising a lipolytic enzyme variant of the invention.
Type:
Grant
Filed:
October 11, 2013
Date of Patent:
July 17, 2018
Assignee:
DANISCO US INC.
Inventors:
Thomas P. Graycar, Sina Pricelius, Ayrookaran J. Poulose, David A. Estell
Abstract: Provided herein are variants of Buttiauxella sp. phytases that may be used in industrial applications including methods for starch liquefaction, alcohol fermentations and for enhancing phosphate digestion in foods and animal feeds.
Type:
Grant
Filed:
June 8, 2017
Date of Patent:
June 19, 2018
Assignee:
DANISCO US INC
Inventors:
Oliver Kensch, Klaus Schulze Pellengahr, Birgitta Leuthner, Jayarama K Shetty, Michael J. Pepsin, Soeren Dalsgaard, Mai Faurschou Isaksen
Abstract: Disclosed are compositions and methods relating to variant alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.
Type:
Application
Filed:
February 2, 2017
Publication date:
June 14, 2018
Applicant:
Danisco US Inc.
Inventors:
William Cuevas, Vivek Sharma, David E. Wildes, Sang-kyu Lee, Dina Finan
Abstract: The present invention provides methods and compositions for the production of mature proteases in bacterial host cells. The compositions include polynucleotides encoding serine protease sequences with modified or heterologous propeptide regions; polypeptides comprising serine proteases with modified or heterologous propeptide regions; expression cassettes, DNA constructs, vectors, and chromosomes comprising such polynucleotides; and bacterial host cells comprising such polynucleotides. The methods include methods for enhancing the production of mature proteases in bacterial host cells (e.g. Bacillus sp. host cells). The produced proteases find use in the industrial production of enzymes, suitable for use in various industries, including but not limited to the cleaning, animal feed and textile processing industry.
Type:
Application
Filed:
June 17, 2016
Publication date:
June 7, 2018
Applicant:
DANISCO US INC.
Inventors:
Lilia Maria BABE, Carol Marie FIORESI, Frits GOEDEGEBUUR, Harm MULDER
Abstract: Described herein is a composition useful for inducing expression of genes whose expression is under control of an inducible promoter sequence and methods for the compositions preparation and use.
Type:
Application
Filed:
July 7, 2016
Publication date:
May 31, 2018
Applicant:
DANISCO US INC.
Inventors:
Timothy C. DODGE, Ilkka Ilari KRUUS, Colin MITCHINSON, Timo Tapio VILJAVA
Abstract: The present invention provides methods and compositions comprising at least one thermolysin-like neutral protease enzyme with improved storage stability and/or catalytic activity. In some embodiments, the thermolysin finds use in cleaning and other applications comprising detergent. In some particularly preferred embodiments, the present invention provides methods and compositions comprising thermolysin formulated and/or engineered to resist detergent-induced inactivation.
Type:
Grant
Filed:
July 8, 2015
Date of Patent:
May 22, 2018
Assignee:
DANISCO US INC.
Inventors:
David A. Estell, Ronaldus W. J. Hommes, Amy D. Liu, Andrew Shaw